CN103988607A - Method for promoting germination of lithocarpus polystachyus rehd seeds - Google Patents
Method for promoting germination of lithocarpus polystachyus rehd seeds Download PDFInfo
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- CN103988607A CN103988607A CN201410220346.2A CN201410220346A CN103988607A CN 103988607 A CN103988607 A CN 103988607A CN 201410220346 A CN201410220346 A CN 201410220346A CN 103988607 A CN103988607 A CN 103988607A
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Abstract
The invention discloses a method for promoting germination of lithocarpus polystachyus rehd seeds. The method is carried out through the steps of collecting seeds, pretreating the seeds, preparing a sand bed, performing sand storage for accelerating germination and performing breeding management and protection. Specifically, the method specifically comprises the following steps: collecting mature lithocarpus polystachyus rehd seeds in October and November, performing seed selection, seed washing, sterilization and gibberellin solution seed soaking treatment, performing sand storage for accelerating germination, and performing breeding management and protection to obtain germinated lithocarpus polystachyus rehd seeds. The dormancy period of the lithocarpus polystachyus rehd seeds is broken through by using gibberellin solution, the germination period of the seeds is obviously shortened, the germination rate of the lithocarpus polystachyus rehd seeds is improved, the germination time of the lithocarpus polystachyus rehd seeds is advanced for nearly 20 days, and the germination rate is improved by over 20 percent. The method is simple in process operation and low in cost and has high economic benefits, social benefits and ecological benefits, and rapid, neat and high-efficiency germination of the lithocarpus polystachyus rehd seeds can be promoted.
Description
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method that promotes Pasania cuspidata seed germination.
Background technology
Pasania cuspidata (
lithocarpus polystachyus Rehd) have another name called sweet tea, be Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) aiphyllium, be China's Traditional Folk herbal medicine and health drink plant.Its leaf contains sweet theine, is Gao Tian, low-heat, avirulent natural sweetener.Research in recent years confirms that Pasania cuspidata leaf is also rich in 17 seed amino acids, various trace elements, the vitamin of needed by human, the compound compositions such as phloridzin, trifloroside and multiple flavonoids and polyphenol, triterpene class, there is the human free radical, anti-ageing, antiallergy of removing, hemangiectasis, regulate vascular permeability, prevent arteriosclerosis, the pharmacological action such as hypotensive, lipopenicillinase, diuresis, anti-inflammation, inhibition tumor cell.All there is certain effect for prevention of obesity disease, diabetes, cardiovascular disease, high blood pressure, arteriosclerosis, carious tooth etc.
At present, Pasania cuspidata resource is still in wild distribution, but along with growing Chinese medicine consumption demand, large-scale artificial cultivation Pasania cuspidata resource is imperative.Because Pasania cuspidata seed belongs to hardseed, in natural germination process, there is several factors to cause seed germination success rate low, the reduction of resource is not supported in the sprouting naturally of seed far away.And often only have 50% left and right without the Pasania cuspidata percentage of seedgermination of any hormone agents processing in traditional mode of production, and germinating time evening, emerge irregularly, limit the large-scale production of Pasania cuspidata seedling.
Summary of the invention
Object of the present invention is the deficiency that overcomes existing Pasania cuspidata seed sprouting technology, provides a kind of and can improve the germination rate of Pasania cuspidata seed and accelerate sprouting speed, workable, practical simplicity, the method for the promotion Pasania cuspidata seed germination that cost is low.
The present invention is achieved by the following technical solutions:
A kind of method that promotes Pasania cuspidata seed germination, comprise the operation that seed collection, seed pretreatment, husky bed preparation, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Pasania cuspidata seed, choose seeds, wash kind, implement the husky vernalization of hiding after sterilization and seed soaking, manage and protect the seed that obtains germination by breeding, main operational steps is as follows:
(1) seed collection: 10～November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed;
(2) seed pretreatment:
1. seed selection: the fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form;
2. wash kind: the Pasania cuspidata seed clear water of choosing is soaked to 40～50h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained;
3. sterilization: to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 50～70min by cleaning Pasania cuspidata seed after draining, then pulls out, dries in the shade after rinsing well with clear water;
4. seed soaking: the Gibberellins solution of the Pasania cuspidata seed after sterilization being put into 100～600mg/L is soaked 40～50h;
(3) husky bed is prepared: in husky bed, first spread the fresh clean thin river sand that 8～12cm is thick, it is that 0.5～1% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand;
(4) husky Tibetan vernalization: by the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even being spread out on husky bed, then cover clean thin river sand, the water of then fully drenching, finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film again;
(5) breeding is managed and protected: seed is husky hide during to husky bed periodically sprinkle bactericide, and according to moisture evaporation situation in sand, spray a small amount of clear water in good time, keep the suitable humidity of sand.
Preferably, the husky bed described in above step (3) is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 30～40cm.
Preferably, the described even stand thickness in above step (4) is no more than 20cm; The thickness of the described clean thin river sand of covering is 4～5cm.
Preferably, the periodically sprinkle bactericide described in above step (5) be with volumetric concentration be 1 ‰ carbendazim solutions, spray once husky bed every 12～18d; Described suitable humidity is that sand is being held humidity agglomerating, that loose one's grip and scatter.
The present invention is with respect to prior art, and the advantage and the good effect that have are as follows:
1, the method for promotion Pasania cuspidata seed germination of the present invention, do not need to remove base portion seed coat, remove the not easy-operating modes such as part cotyledon through mechanical damage, only need to soak and Sand culture by the Gibberellins solution of suitable concentration, just can effectively break seed dormancy, accelerate seed germination and improve germination rate, sprout in advance nearly 20 days than the Pasania cuspidata seed without any hormone agents processing in traditional mode of production, germination rate improves more than 20%.
2, the method for promotion Pasania cuspidata seed germination of the present invention, Pasania cuspidata seed is processed and buries vernalization with fine sand through gibberellin, sprouts and shortens perdurabgility, and germination peak period is relatively concentrated, bud seedling high-quality, whole structure is good.
3, the method for promotion Pasania cuspidata seed germination of the present invention, technological operation is simple, with low cost, and handled easily is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1:
In early October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form.The Pasania cuspidata seed clear water of choosing is soaked to 40h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained.By cleaning Pasania cuspidata seed after draining, to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 50min, then pulls out, after rinsing well, dries in the shade with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization is put into 100mg/L is soaked 40h and is impelled germination with the resting stage of breaking seed; During seed soaking, get in advance husky bed ready, husky bed is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 30cm.In husky bed, first spread the fresh clean thin river sand that 8cm is thick, it is that 0.5% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even to be spread out in husky bed upper, and even stand thickness is no more than 20cm; Then cover clean thin river sand, the thickness that covers clean thin river sand is 4cm again, and the water of then fully drenching finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film.During the husky Tibetan of seed, regularly husky bed being sprayed to a volumetric concentration every 12d is 1 ‰ carbendazim solution bactericide, and according to moisture evaporation situation in sand, sprays a small amount of clear water, the suitable humidity of maintenance sand in good time; The suitable humidity of sand is that sand is being held humidity agglomerating, that loose one's grip and scatter, until seed sprouting, the germination rate of seed is 81.7%.
Embodiment 2:
Mid-October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form.The Pasania cuspidata seed clear water of choosing is soaked to 48h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained.By cleaning Pasania cuspidata seed after draining, to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 60min, then pulls out, after rinsing well, dries in the shade with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization is put into 200mg/L is soaked 48h and is impelled germination with the resting stage of breaking seed; During seed soaking, get in advance husky bed ready, husky bed is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 35cm.In husky bed, first spread the fresh clean thin river sand that 9cm is thick, it is that 1% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even to be spread out in husky bed upper, even stand thickness 10cm; Then cover clean thin river sand, the thickness that covers clean thin river sand is 4cm again, and the water of then fully drenching finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film.During the husky Tibetan of seed, regularly husky bed being sprayed to a volumetric concentration every 14d is 1 ‰ carbendazim solution bactericide, and according to moisture evaporation situation in sand, sprays a small amount of clear water, the suitable humidity of maintenance sand in good time; The suitable humidity of sand is that sand is being held humidity agglomerating, that loose one's grip and scatter, until seed sprouting, the germination rate of seed is 84.7%.
Embodiment 3:
In early November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form.The Pasania cuspidata seed clear water of choosing is soaked to 48h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained.By cleaning Pasania cuspidata seed after draining, to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 60min, then pulls out, after rinsing well, dries in the shade with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization is put into 400mg/L is soaked 48h and is impelled germination with the resting stage of breaking seed; During seed soaking, get in advance husky bed ready, husky bed is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 35cm.In husky bed, first spread the fresh clean thin river sand that 10cm is thick, it is that 1% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even to be spread out in husky bed upper, and even stand thickness is 15cm; Then cover clean thin river sand, the thickness that covers clean thin river sand is 5cm again, and the water of then fully drenching finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film.During the husky Tibetan of seed, regularly husky bed being sprayed to a volumetric concentration every 15d is 1 ‰ carbendazim solution bactericide, and according to moisture evaporation situation in sand, sprays a small amount of clear water, the suitable humidity of maintenance sand in good time; The suitable humidity of sand is that sand is being held humidity agglomerating, that loose one's grip and scatter, until seed sprouting, the germination rate of seed is 76.7%.
Embodiment 4:
In late October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form.The Pasania cuspidata seed clear water of choosing is soaked to 50h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained.By cleaning Pasania cuspidata seed after draining, to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 70min, then pulls out, after rinsing well, dries in the shade with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization is put into 600mg/L is soaked 50h and is impelled germination with the resting stage of breaking seed; During seed soaking, get in advance husky bed ready, husky bed is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 40cm.In husky bed, first spread the fresh clean thin river sand that 12cm is thick, it is that 1% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even to be spread out in husky bed upper, and even stand thickness is 20cm; Then cover clean thin river sand, the thickness that covers clean thin river sand is 5cm again, and the water of then fully drenching finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film.During the husky Tibetan of seed, regularly husky bed being sprayed to a volumetric concentration every 18d is 1 ‰ carbendazim solution bactericide, and according to moisture evaporation situation in sand, sprays a small amount of clear water, the suitable humidity of maintenance sand in good time; The suitable humidity of sand is that sand is being held humidity agglomerating, that loose one's grip and scatter, until seed sprouting, the germination rate of seed is 78.3%.
Claims (4)
1. one kind promotes the method for Pasania cuspidata seed germination, it is characterized in that: comprise the operation that seed collection, seed pretreatment, husky bed preparation, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Pasania cuspidata seed, choose seeds, wash kind, implement the husky vernalization of hiding after sterilization and seed soaking, manage and protect the seed that obtains germination by breeding, main operational steps is as follows:
(1) seed collection: 10～November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard enforcement when bright collected seed, and obtains fresh Pasania cuspidata seed;
(2) seed pretreatment:
1. seed selection: the fresh Pasania cuspidata seed collecting is spread to indoor ventilating, without direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and remove worm channel, form not just, have damage and a too small seed of form;
2. wash kind: the Pasania cuspidata seed clear water of choosing is soaked to 40～50h, make its abundant imbibition; In clear water, fully stir again Pasania cuspidata seed, then leave standstill, remove the floater or the ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drained;
3. sterilization: to put into volumetric concentration be that 0.5% liquor potassic permanganate soaks 50～70min by cleaning Pasania cuspidata seed after draining, then pulls out, dries in the shade after rinsing well with clear water;
4. seed soaking: the Gibberellins solution of the Pasania cuspidata seed after sterilization being put into 100～600mg/L is soaked 40～50h;
(3) husky bed is prepared: in husky bed, first spread the fresh clean thin river sand that 8～12cm is thick, it is that 0.5～1% liquor potassic permanganate carries out disinfection that sowing the previous day fully sprays volumetric concentration to thin river sand;
(4) husky Tibetan vernalization: by the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, even being spread out on husky bed, then cover clean thin river sand, the water of then fully drenching, finally covers a husky surface and carries out moisturizing with being clear to translucent clean white plastic film again;
(5) breeding is managed and protected: seed is husky hide during to husky bed periodically sprinkle bactericide, and according to moisture evaporation situation in sand, spray a small amount of clear water in good time, keep the suitable humidity of sand.
2. a kind of method that promotes Pasania cuspidata seed germination according to claim 1, is characterized in that: the husky bed described in step (3) is selected at and leewardly on the sunny side, is built into fragment of brick on well-drained level land, and the height of husky bed is 30～40cm.
3. a kind of method that promotes Pasania cuspidata seed germination according to claim 1, is characterized in that: the described even stand thickness in step (4) is no more than 20cm; The thickness of the described clean thin river sand of covering is 4～5cm.
4. a kind of method that promotes Pasania cuspidata seed germination according to claim 1, is characterized in that: the periodically sprinkle bactericide described in step (5) be with volumetric concentration be 1 ‰ carbendazim solutions, spray once husky bed every 12～18d; Described suitable humidity is that sand is being held humidity agglomerating, that loose one's grip and scatter.
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Cited By (9)
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| CN105265059A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus oleifolius A. camus |
| CN105265062A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus elmerrillii |
| CN105265058A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for improving germination rate of Lithocarpus craibianus seeds |
| CN105284236A (en) * | 2015-11-04 | 2016-02-03 | 张莘蔓 | Method for promoting germination of quercus acutissima seeds |
| CN105284406A (en) * | 2015-11-30 | 2016-02-03 | 金陵科技学院 | Simple accelerating germination technology for Cornus walteri seeds |
| CN105325096A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting germination of lithocarpus amygdalifolius seeds |
| CN105325097A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting seed germination of lithocarpus calophyllus |
| CN107231911A (en) * | 2017-05-24 | 2017-10-10 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of method for improving extremely frigid zones grass vetch yield |
| CN107306533A (en) * | 2017-06-19 | 2017-11-03 | 四川森迪科技发展股份有限公司 | A kind of Idesia polycarpa method for treating seeds of high bud ratio |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265059A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus oleifolius A. camus |
| CN105265062A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus elmerrillii |
| CN105265058A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for improving germination rate of Lithocarpus craibianus seeds |
| CN105284236A (en) * | 2015-11-04 | 2016-02-03 | 张莘蔓 | Method for promoting germination of quercus acutissima seeds |
| CN105325096A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting germination of lithocarpus amygdalifolius seeds |
| CN105325097A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting seed germination of lithocarpus calophyllus |
| CN105284406A (en) * | 2015-11-30 | 2016-02-03 | 金陵科技学院 | Simple accelerating germination technology for Cornus walteri seeds |
| CN105284406B (en) * | 2015-11-30 | 2018-08-14 | 金陵科技学院 | A kind of seed simple germination method of mao of Lai seed |
| CN107231911A (en) * | 2017-05-24 | 2017-10-10 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of method for improving extremely frigid zones grass vetch yield |
| CN107306533A (en) * | 2017-06-19 | 2017-11-03 | 四川森迪科技发展股份有限公司 | A kind of Idesia polycarpa method for treating seeds of high bud ratio |
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