CN109005744A - A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin - Google Patents
A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin Download PDFInfo
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- CN109005744A CN109005744A CN201811077142.2A CN201811077142A CN109005744A CN 109005744 A CN109005744 A CN 109005744A CN 201811077142 A CN201811077142 A CN 201811077142A CN 109005744 A CN109005744 A CN 109005744A
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- seed
- cotton
- callicarpa
- gibberellin
- bodinieri levl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a kind of methods for improving Callicarpa bodinieri Levl. seed germination rate using gibberellin, and steps are as follows: a, the first dry pericarp in removal Callicarpa Pedunculata R. br. fruit outside remove clast using wet cleaning process and in order to kind；B, the obtained seed of step a is immersed in 8-15min in 0.2-0.4g/L liquor potassic permanganate under normal temperature condition, clear water rinses seed, impregnates seed with warm water later, then seed is placed in 0.0005-0.005g/L Gibberellins solution and impregnates 36-48h；C, the seed handled through step b is laid on the cotton or absorbent cotton sterilized by the liquor natrii hypochloritis that mass concentration is 5.5-6.5%, seed is placed under 20-25 DEG C of ventilated environment and carries out vernalization, cotton or absorbent cotton overdrying or overly moist are prevented, and removes mouldy seed as early as possible.Speed of germination is fast, and germination percentage is high.The relatively existing Callicarpa bodinieri Levl. seed sprout time of the present invention is advanced by 1-2 days or so, and seed germination rate is up to 93.64%.
Description
Technical field
The present invention relates to forestry nursery stock Cultivating techniques fields.It is more particularly related to which a kind of mentioned using gibberellin
The method of high Callicarpa bodinieri Levl. seed germination rate.
Background technique
Callicarpa bodinieri Levl. (Callicarpa bodinieri Levl.) is Verenaceae, Callicarpa machaka, and Bioclimatic analysis is wide,
With compared with Gao Guanhua, see the ornamental values such as fruit, and there is certain pharmaceutical value, distributing region is East China, China at home
The ground such as south.Traditional nursery of Callicarpa bodinieri Levl. mainly has two methods of seeding and seedling raising and cuttage and seedling culture, and seeding and seedling raising has sowing germination percentage
Low, the problems such as speed of germinating is slow；Although cuttage and seedling culture high survival rate, limited by breeding material quantity, it cannot a large amount of nursery.
By consulting literatures, it is found that it is less Callicarpa bodinieri Levl. seed sprouts research, and the callicarpa nudiflora seed of its subspecies sprout research compared with
It is more.Callicarpa nudiflora country's distributing region is mainly that Jiangxi, Fujian, Guangdong, Guangxi and Hainan etc. are regional, compared with Callicarpa bodinieri Levl. habitat
Latitude is lower, temperature is higher, moisture is sufficient.
An Puyuan and Sun Xiuqin directly sows Callicarpa bodinieri Levl. seed, does not carry out any processing, warm round the clock in seed germination
Degree is 13/30 DEG C and non-illuminated conditions, and germination percentage is up to 66%；Diurnal temperature is 20/30 DEG C and non-illuminated conditions, and seed is sprouted
Sending out germinating time is 9 days, germination percentage 65%.
Patent publication No. is in a kind of " method of rapid release of Callicarpa nudiflora seed dormancy " of CN102845158A to naked
Flower Callicarpa bodinieri Levl. carries out gibberellin and handles seed, and gibberellin concentration is 100-300mg/L, and seed culture temperature is 30 DEG C, and seed starts
Sprout time is 5 days, and germination rate is 81.0% ± 2.0%.
Wang Chunmei etc., to callicarpa nudiflora fresh fruit carry out clear water wash by rubbing with the hands, after removing kind of shell, dried, then 7 DEG C it is cold
After processing 3 days, the sowing of room temperature in March is impregnated 3 hours before sowing with 1500 times of carbendazim, and dry sand is mixed thoroughly, earthing 3mm, and the 8th day
Start to germinate, bud ratio 80.2%.
In existing Callicarpa bodinieri Levl. and its callicarpa nudiflora Seed Germination of subspecies, there are following several respects shortcomings for discovery:
1, seed Germination Temperature environment is higher, and the self seed time postpones；
2, Callicarpa bodinieri Levl. percentage of seedgermination is lower, and sprout time is long.
Summary of the invention
The technical problems to be solved by the invention are as follows: how to shorten Callicarpa bodinieri Levl. seed sprout time, improve asking for its germination rate
Topic.
The technical solution of the present invention is as follows: a kind of method for improving Callicarpa bodinieri Levl. seed germination rate using gibberellin, steps are as follows:
A, dry pericarp first outside removal Callicarpa Pedunculata R. br. fruit removes clast using wet cleaning process and in order to kind, retains lower layer and sink to the bottom
Seed；
B, the obtained seed of step a is immersed in 8-15min in 0.2-0.4g/L liquor potassic permanganate under normal temperature condition, so
Seed is rinsed using clear water afterwards, seed 28-35min is impregnated with 40-50 DEG C of warm water later, seed is then placed in 0.0005-
36-48h is impregnated in 0.005g/L Gibberellins solution；
C, the seed handled through step b is laid in and is sterilized by the liquor natrii hypochloritis that mass concentration is 5.5-6.5%
Cotton or absorbent cotton on, rinse it is clean after, moisten cotton or absorbent cotton with clear water, guarantee Callicarpa bodinieri Levl. seed fraction kind crestal line always
Contact water；Seed is placed under 20-25 DEG C of ventilated environment and carries out vernalization, prevents cotton or absorbent cotton overdrying or overly moist, and to the greatest extent
It is early to remove mouldy seed.
Using method of the invention, the Callicarpa bodinieri Levl. seed treatment time is 1.5-2d or so, the 6-7 days beginning kinds after vernalization
Son shows money or valuables one carries unintentionally, and sprouting peak period is 12-16 days, germination rate 93.64%, goes mouldy and occurs almost without seed in entire pregermination procedure.
Compared with prior art, the invention has the following advantages:
1, seed Germination Temperature is lower, is suitable for early spring seeding and seedling raising condition, and a situation arises does not almost have for seed mildew
Have.
2, speed of germination is fast, and germination percentage is high.The relatively existing Callicarpa bodinieri Levl. seed sprout time of the present invention is advanced by 1-2 days or so, plants
Sub- germination rate is up to 93.64%.
Detailed description of the invention
Fig. 1 is influence of the different gibberellin concentration to Callicarpa bodinieri Levl. germination percentage.
Specific embodiment
Embodiment 1
Dry pericarp outside Callicarpa Pedunculata R. br. fruit is removed, 4 meshes is crossed and the seed after sieving is submerged in water, using wet cleaning process
The seed of heavy water and separating in order to kind etc. is obvious for floating are asked, upper layer float with tool clast and in order to kind of a removal, reservation lower layer
The seed sunk to the bottom；Configuration concentration is 0.2g/L potassium permanganate antiseptic solution, impregnates seed 8min under normal temperature condition, is rushed using clear water
Wash water of the seed to after cleaning without kermesinus subject to, later with 40 DEG C of warm water immersion seed 35min, then seed is placed in
36h is impregnated in 0.002g/L Gibberellins solution；Seed after immersion is laid in the sodium hypochlorite for being 5.5% by mass concentration
On the cotton or absorbent cotton of solution disinfection, after rinsing completely, cotton or absorbent cotton is moistened with clear water, guarantees Callicarpa bodinieri Levl. seed fraction kind
Crestal line contacts water always；Seed is placed under 20 DEG C of ventilated environments and carries out vernalization, periodically checks that cotton or absorbent cotton prevented
Situation dry or overly moist, and mouldy seed is removed as early as possible.
As a result: seed shows money or valuables one carries unintentionally the time for 7.5d, and sprouting peak period is 14-19d, germination percentage 87.28%
Embodiment 2
Dry pericarp outside Callicarpa Pedunculata R. br. fruit is removed, 4 meshes is crossed and the seed after sieving is submerged in water, using wet cleaning process
The seed of heavy water and separating in order to kind etc. is obvious for floating are asked, upper layer float with tool clast and in order to kind of a removal, reservation lower layer
The seed sunk to the bottom；Configuration concentration is 0.4g/L potassium permanganate antiseptic solution, impregnates seed 15min or so under normal temperature condition, utilizes
Clear water rinse water of the seed to after cleaning without kermesinus subject to, later with 50 DEG C of warm water immersion seed 28min, then seed is set
48h is impregnated in 0.002g/L Gibberellins solution；Seed after immersion is laid in the hypochlorous acid for being 6.5% by mass concentration
On the cotton or absorbent cotton of sodium solution disinfection, after rinsing completely, cotton or absorbent cotton is moistened with clear water, guarantees Callicarpa bodinieri Levl. seed fraction
Kind crestal line contacts water always；Seed is placed under 25 DEG C of ventilated environments and carries out vernalization, periodically checks that cotton or absorbent cotton prevent
Overdrying or situation overly moist, and mouldy seed is removed as early as possible.
As a result: seed shows money or valuables one carries unintentionally the time for 6.5-7.5d, and sprouting peak period is 13-18d, germination percentage 92.41%
Embodiment 3
Dry pericarp outside Callicarpa Pedunculata R. br. fruit is removed, 4 meshes is crossed and the seed after sieving is submerged in water, using wet cleaning process
The seed of heavy water and separating in order to kind etc. is obvious for floating are asked, upper layer float with tool clast and in order to kind of a removal, reservation lower layer
The seed sunk to the bottom；Configuration concentration is 0.3g/L potassium permanganate antiseptic solution, impregnates seed 10min under normal temperature condition, utilizes clear water
Rinse water of the seed to after cleaning without kermesinus subject to, later with 45 DEG C of warm water immersion seed 30min, then seed is placed in
42h is impregnated in 0.002g/L Gibberellins solution；It is molten that seed after immersion is laid in the sodium hypochlorite for being 6% by mass concentration
On the cotton or absorbent cotton of liquid disinfectant, after rinsing completely, cotton or absorbent cotton is moistened with clear water, guarantees Callicarpa bodinieri Levl. seed fraction kind ridge
Line contacts water always；Seed is placed under 23 DEG C of ventilated environments and carries out vernalization, periodically checks that cotton or absorbent cotton prevent overdrying
Or situation overly moist, and mouldy seed is removed as early as possible.
As a result: seed shows money or valuables one carries unintentionally the time for 6d, and sprouting peak period is 12-16d, germination percentage 93.64%
Comparative example
In order to study influence of the gibberellin concentration to Callicarpa bodinieri Levl. seed germination rate, on the basis of embodiment 3, change gibberellin
Concentration, successively value be 0g/L, 0.0005g/L, 0.001g/L, 0.002g/L, 0.005g/L, 0.01g/L, 0.02g/L,
0.05g/L, 0.1g/L, 0.2g/L, as a result such as table 1:
Influence of the different gibberellin concentration of table 1 to Callicarpa bodinieri Levl. germination percentage
In Publication No. CN102845158A, nakedflower the conclusion of the gradient experiment of gibberellin: is handled with gibberellin aqueous solution
Callicarpa bodinieri Levl. seed can shorten the sprout time of callicarpa nudiflora seed, but not make significant difference to callicarpa nudiflora percentage of seedgermination.
The study result show that conclusion in above-mentioned patent and incorrect, when gibberellin concentration is higher
The germination percentage variation of (0.1-0.3g/L or 0.02-0.2g/L of the invention in CN102845158A), Callicarpa bodinieri Levl. is not significant, but
When gibberellin concentration is in 0.0005-0.005g/L, germination percentage is significantly improved, especially when gibberellin concentration is in 0.002g/L
When, reach 93.64%.It can be seen that the gibberellin of low concentration can significantly improve the germination percentage of Callicarpa bodinieri Levl..
Claims (2)
1. a kind of method for improving Callicarpa bodinieri Levl. seed germination rate using gibberellin, which is characterized in that steps are as follows:
A, dry pericarp first outside removal Callicarpa Pedunculata R. br. fruit removes clast using wet cleaning process and in order to kind, retains the kind that lower layer sinks to the bottom
Son；
B, the obtained seed of step a is immersed in 8-15min in 0.2-0.4g/L liquor potassic permanganate under normal temperature condition, it is then sharp
Seed is rinsed with clear water, seed 28-35min is impregnated with 40-50 DEG C of warm water later, seed is then placed in 0.0005-0.005g/
36-48h is impregnated in L Gibberellins solution；
C, the seed handled through step b is laid in the cotton sterilized by the liquor natrii hypochloritis that mass concentration is 5.5-6.5%
On cloth or absorbent cotton, after rinsing completely, cotton or absorbent cotton are moistened with clear water, guarantees that Callicarpa bodinieri Levl. seed fraction kind crestal line contacts always
Water；Seed is placed under 20-25 DEG C of ventilated environment and carries out vernalization, prevents cotton or absorbent cotton overdrying or overly moist, and go as early as possible
Except mouldy seed.
2. a kind of method for improving Callicarpa bodinieri Levl. seed germination rate using gibberellin according to claim 1, which is characterized in that step
It is rapid as follows:
A, dry pericarp first outside removal Callicarpa Pedunculata R. br. fruit removes clast using wet cleaning process and in order to kind, retains the kind that lower layer sinks to the bottom
Son；
B, configuration concentration is 0.3g/L potassium permanganate antiseptic solution, the seed 10min that soaking step a is obtained under normal temperature condition, so
Seed is rinsed using clear water afterwards, seed 30min is impregnated with 45 DEG C of warm water later, it is molten that seed is then placed in 0.002g/L gibberellin
42h is impregnated in liquid；
C, the seed handled through step b is laid in the cotton sterilized by the liquor natrii hypochloritis that mass concentration is 6% or taken off
On rouge cotton, after rinsing completely, cotton or absorbent cotton are moistened with clear water, guarantees that Callicarpa bodinieri Levl. seed fraction kind crestal line contacts water always；It will
Seed is placed under 23 DEG C of ventilated environments and carries out vernalization, prevents cotton or absorbent cotton overdrying or overly moist, and remove mouldy kind as early as possible
Son.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811077142.2A CN109005744B (en) | 2018-09-15 | 2018-09-15 | Method for improving germination rate of callicarpa bodinieri seeds by using gibberellin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811077142.2A CN109005744B (en) | 2018-09-15 | 2018-09-15 | Method for improving germination rate of callicarpa bodinieri seeds by using gibberellin |
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| Publication Number | Publication Date |
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| CN109005744A true CN109005744A (en) | 2018-12-18 |
| CN109005744B CN109005744B (en) | 2020-10-30 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004054363A1 (en) * | 2002-12-16 | 2004-07-01 | Council Of Scientific And Industrial Research | Use of phyllocladane diterpenoids for plant growth promotion and alleviation of growth retardant allelochemicals, and method therefor |
| CN102845158A (en) * | 2012-08-27 | 2013-01-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapid release of Callicarpa nudiflora seed dormancy |
| CN103004323A (en) * | 2013-01-16 | 2013-04-03 | 广西壮族自治区林业科学研究院 | Method for promoting germination of cinnamon seed |
| CN105265059A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus oleifolius A. camus |
| CN108029278A (en) * | 2017-12-08 | 2018-05-15 | 甘肃省治沙研究所 | A kind of method that Picea Mongolica seed germination rate is improved using gibberellin |
| CN108496796A (en) * | 2018-03-09 | 2018-09-07 | 浙江省亚热带作物研究所 | A kind of Callicarpa bodinieri Levl. aseptic seeding rapid propagation method |
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2018
- 2018-09-15 CN CN201811077142.2A patent/CN109005744B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004054363A1 (en) * | 2002-12-16 | 2004-07-01 | Council Of Scientific And Industrial Research | Use of phyllocladane diterpenoids for plant growth promotion and alleviation of growth retardant allelochemicals, and method therefor |
| CN102845158A (en) * | 2012-08-27 | 2013-01-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapid release of Callicarpa nudiflora seed dormancy |
| CN103004323A (en) * | 2013-01-16 | 2013-04-03 | 广西壮族自治区林业科学研究院 | Method for promoting germination of cinnamon seed |
| CN105265059A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus oleifolius A. camus |
| CN108029278A (en) * | 2017-12-08 | 2018-05-15 | 甘肃省治沙研究所 | A kind of method that Picea Mongolica seed germination rate is improved using gibberellin |
| CN108496796A (en) * | 2018-03-09 | 2018-09-07 | 浙江省亚热带作物研究所 | A kind of Callicarpa bodinieri Levl. aseptic seeding rapid propagation method |
Non-Patent Citations (4)
| Title |
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| CRISTINA MARIA: "Gibbrellic Acid production", 《CURRENT DEVELOPMENT IN SOLID-STATE FERMENTATION》 * |
| 中国农业百科全书编辑部编: "《中国农业百科全书（农药卷）》", 28 October 1993 * |
| 王春梅 等: "海南黎药—裸花紫珠种子育苗技术研究", 《中国农学通报》 * |
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