CN104106327A - Method for rapidly releasing dormancy of brucea javanica seeds - Google Patents
Method for rapidly releasing dormancy of brucea javanica seeds Download PDFInfo
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- CN104106327A CN104106327A CN201410292518.7A CN201410292518A CN104106327A CN 104106327 A CN104106327 A CN 104106327A CN 201410292518 A CN201410292518 A CN 201410292518A CN 104106327 A CN104106327 A CN 104106327A
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- 235000010889 Rhus javanica Nutrition 0.000 title claims abstract description 96
- 238000000034 method Methods 0.000 title claims abstract description 39
- 241001533159 Brucea javanica Species 0.000 title claims abstract 22
- 230000005059 dormancy Effects 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000004576 sand Substances 0.000 claims abstract description 29
- 238000012545 processing Methods 0.000 claims abstract description 28
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 27
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 22
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000003448 gibberellin Substances 0.000 claims abstract description 22
- 239000007864 aqueous solution Substances 0.000 claims abstract description 15
- 238000011282 treatment Methods 0.000 claims description 19
- 230000008569 process Effects 0.000 claims description 15
- 238000003860 storage Methods 0.000 claims description 13
- 241000737241 Cocos Species 0.000 claims description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 7
- 238000007598 dipping method Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 238000010129 solution processing Methods 0.000 claims description 6
- 230000035800 maturation Effects 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 2
- 239000010455 vermiculite Substances 0.000 claims description 2
- 229910052902 vermiculite Inorganic materials 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 2
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- 244000097577 Rhus javanica Species 0.000 description 76
- 230000035784 germination Effects 0.000 description 35
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 26
- 230000007226 seed germination Effects 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 7
- 239000005708 Sodium hypochlorite Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 6
- 239000008223 sterile water Substances 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
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- 108010051696 Growth Hormone Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
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- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
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- 241000158621 Brucea Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000003085 Quassia amara Species 0.000 description 1
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- 239000002956 ash Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
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- 230000032823 cell division Effects 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for rapidly releasing dormancy of brucea javanica seeds, which comprises the following steps: 1)storing ripe brucea javanica fruits for 7-14 days at low temperature of 4 DEG C; 2)immersing brucea javanica fruits for 1-5 days in warm water at 20-30 DEG C, kneading by using 5wt%-20wt% of fine sand and removing pulp; 3)processing brucea javanica seeds by concentrated sulfuric acid with concentration of 98% for 30-90 seconds, and using running water for flushing until no sulfuric acid is residual; 4)processing brucea javanica seeds by using 100-300mg/L of gibberellin (GA3) aqueous solution for 2-48 hours; and 5) sprouting the processed brucea javanica seeds. The method has the advantages of shortening of seedling growing period and tidiness of seedling, and can be widely used for seedling, breeding and cultivating of medicinal material brucea javanica seeds.
Description
Technical field
The present invention relates to the germination field of Seeds of Medicinal Plants, specifically, the present invention relates to a kind of seed dormant method of Java brucea of removing fast.
Background technology
Java brucea is the dry fruit of quassia Java brucea (Brucea javanica (L.) Merr.Rhusjavanica L.), is the raw material of the situation of selling well Chinese patent drugs such as oleum fructus bruceae soft capsule, brucea fruit oil oral latex emulsion, Java brucea fruit oil emulsion injection.In recent years, the Chinese patent drug taking Java brucea as Effect raw material is evident in efficacy, and demand increases swift and violent, makes Java brucea medicinal raw material price also year after year soaring, raw material supplying wretched insufficiency, and supply shortage is in short supply serious.To the great demand of the Java brucea as medical material, cause wild resource to destroy serious, gather wild resource and can not meet need of production, carrying out standardization production is the effective way that solves medicinal material shortage, and seedling breeding is the important component part that standardization is produced, because fruit of khosam processing maturation period is longer, kernel maturing differs, add that pulp and seed difficulty separate, seed is perishable, and seed coat is hard etc., causes the Java brucea seedling breeding cycle longer, emerge inconsistent, had a strong impact on the standardized cultivation of Java brucea.
By the retrieval to domestic and foreign literature and patent, about Java brucea seed sprouting and sapling multiplication retrieve 1 section of document altogether, (the Li Xianghong such as Li Xianghong, He Fan, Li Hong, Zhu Deling, Gan Ping Chun. Hainan Java brucea culture technique preliminary study. Chinese gardening digest, 2009 (1): 42-45.) Java brucea presprouting of seeds technology has been carried out to simple introduction, this result is thought: Java brucea seed need be through placing a period of time, spend resting stage, then with 50～55 DEG C of hot water seed soaking 15～20min, clean 2～3 times, soak 12～24h with clear water again, and be placed in 33～35 DEG C of environment vernalization, in vernalization process, to keep humidity, after 7～10d, seed germinates successively, reveal bud point when having seen 10%～20%, can sow, but it is irregular that Java brucea germinates, emerge and reach 1 year.
In seed germination experiment, can be divided into physical treatment and chemical treatment two classes according to the difference of its processing method, wherein physical treatment mainly comprises 3 kinds of mechanical treatments, Temperature Treatment, ultrasonic processing, chemical treatment can be divided into 5 classes such as acid treatment, alkaline treatment, oxidizer treatment, salt processing, HORMONE TREATMENT, can be divided into again 3 classes according to mechanism that seed dormancy is abolished: (1) soften seed coat, improve Permeable characteristic between embryo and external environment etc., mainly contain Temperature Treatment, salt acid treatment, sodium bicarbonate and process and 4 kinds of processing modes of hydrogen peroxide processing; (2) nutrition processing, mainly contains ash, calcium chloride processing, potassium nitrate processing, iron chloride processing and boric acid and processes 4 kinds of processing modes; (3) HORMONE TREATMENT, mainly contains growth hormone processing, basic element of cell division processing and gibberellin and processes 3 double recipe formulas.Early-stage Study find to affect Java brucea seed sprouting because have: there is after ripening in (1) fruit and seed, need be through the low temperature storage after-ripening of finishing dealing with; (2) pulp separates with seed is more difficult, easily causes seed to rot, and impact is emerged; (3) seed coat firmly but thinner; (4) seed Inner source GA content is low, need breaking dormancy, comprehensive above-mentioned phenomenon, the present invention has inquired into Java brucea seed by low temperature storage, quartz sand processing, sulfuric acid treatment and gibberellin processing and has abolished the method for dormancy and the research to seed germination thereof.
Summary of the invention
The object of this invention is to provide a kind of seed dormant method of Java brucea of removing fast.
In order to realize object of the present invention, the invention provides a kind of seed dormant method of Java brucea of removing fast, the method comprises the following steps:
Step 1): the fruit of khosam of black maturation is preserved to 7d～14d under 4 DEG C of low temperature, and preferred version is to preserve 10d under 4 DEG C of low temperature;
Step 2): make described step 1) in store fruit of khosam in 20 DEG C～30 DEG C warm water, flood 1d～5d, and rub with the fine sand of 5 % by weight～20 % by weight, to remove pulp, preferred version is to flood after 3d in 25 DEG C of warm water, rubs removal pulp with the fine sand of 10 % by weight;
Step 3): make described step 1) and 2) in Java brucea seed after treatment 98% the concentrated sulfuric acid process 30s～90s, and extremely residual without sulfuric acid with flowing water flushing, after the concentrated sulfuric acid processing 30s that preferred version is 98%, rinse to residual without sulfuric acid with flowing water;
Step 4): make described step 1), 2) and 3) in the gibberellin (GA of Java brucea seed after treatment 100～300mg/L
3) aqueous solution processing 2～48h, preferred version is the gibberellin (GA with 200mg/L
3) aqueous solution processing 6h;
Step 5): make described step 1), 2), 3) and 4) in Java brucea seed after treatment germinate.
Herein, within the scope of the invention, as used in this article, term " clean " refer to wash to chemical treatments in eluent be trace.
As used in this article, term " dipping " refers to the state of seed under liquid level.
Preferably, in described step 5) in, by described step 1) in Java brucea seed after dipping proceed in the illumination box of constant temperature or temperature match curing conditions, in the sand of vermiculite, wet sand, filter paper, sponge germinating bed or mixing 30 % by weight～40 % by weight coconut palm chaffs, germinate.
More preferably, by described step 5) in Java brucea seed after dipping proceed in the illumination box of constant temperature or temperature match curing conditions, mix in the sand of 30 % by weight～40 % by weight coconut palm chaffs and germinate.
The method of quick releasing Java brucea seed dormancy of the present invention, can efficient solution remove the various factors that hinders Java brucea seed germination, comprise that seed and fruit exist after-ripening, pulp and seed difficulty are peeled off, the mechanical obstacles that seed coat forms, break Java brucea seed dormancy, significantly improve percentage of seedgermination, germination rate can reach 85.0%, with tradition without process seedling raising manners compared with, there is the growing-seedling period, seedling neat and consistent of shortening, the advantage such as simple to operate, with low cost, can be widely used in seedling breeding and the cultivation of Java brucea medicinal material.
Embodiment
Following examples are only for the present invention is described, but are not used for limiting the scope of the invention.
Illustrate:
1. the germination test (except embodiment 5) adopting in the following example is husky bed germination test method, specific as follows: the group training that diameter is 9cm is with placing the thick quartz sand through sterilizing of 3cm in blake bottle, water is drenched, unnecessary water is outwelled, taking moisturizing not ponding as degree, choose 100 Java brucea seeds and evenly put into blake bottle, and then cover the thick quartz sand through sterilizing of upper 2cm, then after being built, puts into by blake bottle microcomputerized control illumination box (LRH-250-G11; Guangdong Medical Apparatus and Instruments Factory produces), germinate according to the designed specified temp of each test example (constant temperature or alternating temperature) condition, in whole germination process, all carry out illumination; In germination process, detect 1～2 every day, and suitably supply moisture, and Java brucea seed is counted 1 time after starting to germinate every day.Finding that there is corrupt seed should sort out at any time, if mildew seed > 5% should change the quartz sand in blake bottle in time, and after seed is cleaned with distilled water, puts into blake bottle again and continues to make its germination.
2. with reference to " international seed inspection procedure " (1985), the standard (to grow 1cm as germination) whether germinate as evaluation seed " crop seeds inspection procedure " (1995) etc.
3. percentage of seedgermination refers to germination test final period, and whole seed number of germinateing within the date of regulation account for for planting experimentally sub percentage.In germination rate (%)=fixed date, seed number × 100% is planted experimentally in chitting piece/confession.The germination date in this test is 20 days from germination paper bed being put into case.
4. this experiment attempts to break by several different methods the dormancy of Java brucea seed, but only has ash, gibberellin and growth hormone can break the dormancy of Java brucea seed, and other method example hydrochloric acid processing, hydrogen peroxide processing etc. all can not be removed Java brucea seed dormancy.
Experiment material
This tests the fruit of khosam that Java brucea used (Bruceajavanica (L.) Merr.Rhusjavanica L.) seed is in October, the 2013～2013 black maturation of gathering in cities and counties such as Danzhou, Hainan Province, the Wuzhi Mountain, white sand, Haikous year December, by 6～8 object sieve, can not be for subsequent use by the fruit of 6～8 eye mesh screens, set it as experiment material.
Embodiment 1
Experimental technique
Fruit of khosam is divided into two groups, and one group of blank is preserved respectively 3d, 6d, 10d and 14d for one group under 4 DEG C of low temperature.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7, parallel four groups of each concentration, and 100 every group are placed in 30 DEG C of constant incubator full exposures and cultivate, every 10 days statistics germination rates.
The impact of table 1 low temperature storage on Java brucea seed germination
Experimental result
As shown in table 1, when Java brucea seed is during without low temperature storage, about the 20th day, start to sprout, but germination rate is lower, germination rate is lower than 5.0%; When 40d left and right starts to have one to sprout the phase of concentrating, per day germination rate can be up to 1.1%; Reach peak value at 50d, have the Java brucea seed germination of 31.0% left and right.When Java brucea seed is processed after 3～14d by 4 DEG C of low temperature storages, it sprouts the phase of concentrating and not treated Java brucea seed same period, and in 40d left and right, per day germination rate reaches 1.0% ± 2.0%; Reach peak value at 50d.The germination rate of wherein processing the fruit of khosam of 10d through 4 DEG C of low temperature storages is maximum, reach 47.0% ± 2.0%, so draw to draw a conclusion from this research: low temperature storage can significantly improve Java brucea seed germination time and germination rate, wherein 4 DEG C of low temperature storage 10d are preferred version.
Embodiment 2
Experimental technique
Fruit of khosam is divided into two groups, removes pulp (blank) for one group without fine sand, one group through flooding 1d in 20 DEG C～30 DEG C warm water, and rub removal pulp with the fine sand of 5 % by weight.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7, parallel four groups of each concentration, and 100 every group were placed in 30 DEG C of constant incubator full exposures and cultivate, every statistics germination rate on the 10th.
Whether table 2 removes the impact of pulp on Java brucea seed germination
Experimental result
As shown in table 2, when fruit of khosam is during without Transformatin, about the 10th day, start to sprout, but germination rate is lower, germination rate is lower than 5.0%; Reach peak value at 50d, have the Java brucea seed germination of 27.0% left and right.Fruit of khosam is removed after pulp, reach peak value at 50d, germination rate reaches 37.0% ± 2.0%, so draw to draw a conclusion from this research: fruit of khosam is after fine sand is removed pulp, significantly improve its germination rate, for the preferred version in Java brucea seed treatment, but its on sprout time without impact.
Embodiment 3
Experimental technique
Java brucea seed is divided into 6 groups, and wherein the 1st group is blank; The 2nd group for flooding 1d in 20 DEG C～30 DEG C warm water of fruit of khosam, and rub with the fine sand of 5 % by weight, to remove pulp; The 3rd group for flooding 3d in 20 DEG C～30 DEG C warm water of fruit of khosam, and rub with the fine sand of 5 % by weight, to remove pulp; The 4th group for flooding 3d in 20 DEG C～30 DEG C warm water of fruit of khosam, and rub with the fine sand of 10 % by weight, to remove pulp; The 5th group for flooding 3d in 20 DEG C～30 DEG C warm water of fruit of khosam, and rub with the fine sand of 20 % by weight, to remove pulp; The 6th group for flooding 5d in 20 DEG C～30 DEG C warm water of fruit of khosam, and rub with the fine sand of 20 % by weight, to remove pulp.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7, parallel four groups of each concentration, and 100 every group are placed in 30 DEG C of constant incubator full exposures and cultivate, and add up germination rate and mould variability after 50 days.
The impact of the different pulp removing methods of table 3 on Java brucea seed germination
Experimental result
As shown in table 3, with compared with removal pulp, remove pulp and significantly improved Java brucea percentage of seedgermination, wherein the 4th group, the 5th group and the 6th group are because having the germination rate significantly improving, reduce mould variability, be preferred version, wherein the 4th group process in because needing the warm water processing time short, fine sand usage amount is few, is scheme most preferably.
Embodiment 4
Experimental technique
Java brucea seed is divided into two groups, and one group is in 20 DEG C～30 DEG C warm water, to flood 3d, and rubs with the fine sand of 10 % by weight, removes the Java brucea seed (blank) of pulp; One group is in 20 DEG C～30 DEG C warm water, to flood 3d, and rubs with the fine sand of 10 % by weight, removes the Java brucea seed of pulp and processes respectively 30s, 60s and 90s with 98% the concentrated sulfuric acid again.Rinse pH value=7 through flowing water, then sterilize 15 minutes with 1 % by weight aqueous sodium hypochlorite solution, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group are placed in 30 DEG C of constant incubator full exposures cultivations, after 50 days, add up germination rate, mould variability and lethality.
Table 4 concentrated sulfuric acid is processed the impact on Java brucea seed germination
Experimental result
As shown in table 4, in the time that Java brucea seed is processed without the concentrated sulfuric acid, percentage of seedgermination is 47.0% ± 3.0%, but its mould variability is higher, be 53.0% ± 2.0%, process 30s～90s with the concentrated sulfuric acid, significantly reduce the mould variability of seed, but its seed mortality also increases to 37.0% ± 1.0% from 10.0% ± 1.0%, it is that thinner one deck layer of wood is relevant with the pericarp of Java brucea seed by inference, so draw to draw a conclusion from this research: concentrated sulfuric acid processing can significantly reduce Java brucea percentage of seedgermination, reduce mould variability, but because its seed coat is thinner, the preferred version of concentrated sulfuric acid processing is 98% concentrated sulfuric acid processing 30s.
Embodiment 5
Experimental technique
Java brucea seed is divided into two groups, one group of blank, one group of gibberellin (GA with variable concentrations
3) aqueous solution dipping 24h, then to sterilize 15 minutes with 1 % by weight aqueous sodium hypochlorite solution, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group were placed in 30 DEG C of constant incubator full exposures cultivations, every statistics germination rate on the 10th.
Table 5 gibberellin (GA
3) impact of processing on Java brucea seed germination
Experimental result
As shown in table 5, in the time that Java brucea seed is processed without the gibberellin aqueous solution, about the 20th day, start to sprout, but germination rate is lower, germination rate is lower than 5.0%; When 30-40d left and right starts to have one to sprout the phase of concentrating, per day germination rate can be up to 2.8%; Reach peak value at 50d, have the Java brucea seed germination of 47.0% left and right.Process after 24h when the gibberellin aqueous solution with 100～300mg/L, it just starts to sprout in 10d, since 20d, just start fast-germination, reach peak value at 50d, seed germination rate is 85.0% ± 3.0%, so draw to draw a conclusion from this research: with gibberellin (GA
3) aqueous solution processing Java brucea seed, not only can shorten the sprout time of Java brucea seed, can also improve to a certain extent the germination rate of Java brucea seed, consider the consumption of gibberellin, the gibberellin aqueous solution that most preferably scheme is 200mg/L is processed 24h.
Embodiment 6
Experimental technique
Java brucea seed is divided into two groups, one group of blank, one group of gibberellin (GA with 200mg/L
3) aqueous solution dipping 2h, 6h, 24h and 48h, then to sterilize 15 minutes with 1 % by weight aqueous sodium hypochlorite solution, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group were placed in 30 DEG C of constant incubator full exposures cultivations, every statistics germination rate on the 10th.
Table 6
Experimental result
As shown in table 6, in the time that Java brucea seed is processed without the gibberellin aqueous solution, after 50d, germination rate is 47.0% ± 3.0%, when the gibberellin aqueous solution with 200mg/L is processed after 2-48h, it just starts to sprout in 10d, since 20d, just start fast-germination, reach peak value at 50d, seed germination rate is 85.0% ± 3.0%, so draw to draw a conclusion from this research: with the gibberellin (GA of 200mg/L
3) aqueous solution processing Java brucea seed 2-48h, not only can shorten the sprout time of Java brucea seed, the germination rate that can also improve to a certain extent Java brucea seed, considers the processing time, and the gibberellin aqueous solution that most preferably scheme is 200mg/L is processed 6h.
Embodiment 7
Experimental technique
Java brucea seed is divided into 3 groups, the 1st group, directly Java brucea seed is directly seeded in the cultivation matrix of the farmyard manure, 30 % by weight～40 % by weight coconut palm chaffs, 15 % by weight～20 % by weight vermiculites or fine sand and the 30 % by weight～40 % by weight black earth that are mixed with 15 % by weight～20 % by weight and become thoroughly decomposed; The 2nd group, will be through low temperature storage 10d, in 20 DEG C～30 DEG C warm water, flood 3d, and rub with the fine sand of 10 % by weight, to remove pulp, and then process 30s with 98% the concentrated sulfuric acid and remove residual pulp, 200mg/L gibberellin is processed the Java brucea seed of 24h, is seeded in the cultivation matrix of the farmyard manure, 30 % by weight～40 % by weight coconut palm chaffs, 15 % by weight～20 % by weight vermiculites or fine sand and the 30 % by weight～40 % by weight black earth that are mixed with 15 % by weight～20 % by weight and become thoroughly decomposed; The 3rd group, will be through low temperature storage 10d, in 20 DEG C～30 DEG C warm water, flood 3d, and rub with the fine sand of 10 % by weight, to remove pulp, and then process 30s with 98% the concentrated sulfuric acid and remove residual pulp, 200mg/L gibberellin is processed the Java brucea seed of 6h, is seeded in the cultivation matrix of the farmyard manure, 30 % by weight～40 % by weight coconut palm chaffs, 15 % by weight～20 % by weight vermiculites or fine sand and the 30 % by weight～40 % by weight black earth that are mixed with 15 % by weight～20 % by weight and become thoroughly decomposed; Parallel 4 groups of each processing, was added up emergence rate after 50 days by 100 every group.
Experimental result
In cultivation is processed the 1st group, after 30 days, there is a small amount of Java brucea to emerge, at sowing 30d-40d, to emerge more, 50d is emerged successively, and the cycle is long, and inconsistent; In cultivation is processed the 2nd group, after sowing 20 days, there is Java brucea seed germination, in 40d, percentage of seedgermination can reach 85.0% ± 3.0%, but its seedling is very thin, a little less than growth, is unfavorable for final-period management; In cultivation is processed the 3rd group, after sowing 20 days, have Java brucea seed germination, in 40d, percentage of seedgermination can reach 85.0% ± 3.0%, seedling early growth stalwartness, be applicable to extensive seedling breeding, compared with not treated Java brucea seed, through processing method of the present invention, can improve Java brucea percentage of seedgermination 20%～40%, shorten seed germinating time 15～20d.
Claims (7)
1. remove fast the seed dormant method of Java brucea, the method comprises the following steps:
Step 1): the fruit of khosam of black maturation is preserved to 7d～14d under 4 DEG C of low temperature;
Step 2): make described step 1) in the fruit of khosam of storage in 20 DEG C～30 DEG C warm water, flood 1d～5d, and rub with the fine sand of 5 % by weight～20 % by weight, to remove pulp;
Step 3): make described step 1) and 2) in Java brucea seed after treatment 98% the concentrated sulfuric acid process 30s～90s, and rinse to residual without sulfuric acid with flowing water;
Step 4): make described step 1), 2) and 3) in the gibberellin (GA of Java brucea seed after treatment 100～300mg/L
3) aqueous solution processing 2～48h;
Step 5): make described step 1), 2), 3) and 4) in Java brucea seed after treatment germinate.
2. the seed dormant method of quick releasing Java brucea according to claim 1, is characterized in that: in described step 1) in, the fruit of khosam of black maturation is stored to 10d under 4 DEG C of low temperature.
3. the seed dormant method of quick releasing Java brucea according to claim 1, it is characterized in that: making described step 2) in, fruit of khosam through low temperature storage is flooded after 3d in 25 DEG C of warm water, rub removal pulp with the fine sand of 10 % by weight.
4. the seed dormant method of quick releasing Java brucea according to claim 1, is characterized in that: in described step 3) in, will after processing 30s, rinse to residual without sulfuric acid with flowing water preliminary Java brucea seed 98% the concentrated sulfuric acid of removing pulp.
5. the seed dormant method of quick releasing Java brucea according to claim 1, it is characterized in that: in described step 4) in, will remove the gibberellin (GA of 200mg/L for the Java brucea seed of remaining pulp through low temperature storage, fine sand removal pulp, the concentrated sulfuric acid
3) aqueous solution processing 6h.
6. according to the seed dormant method of quick releasing Java brucea described in any one in claim 1 to 5, it is characterized in that: in described step 5) in, by described step 1), 2), 3) and 4) in the Java brucea seed processed proceed in the illumination box of constant temperature or temperature match curing conditions, in the sand of vermiculite, wet sand, filter paper, sponge germinating bed or mixing 30 % by weight～40 % by weight coconut palm chaffs, germinate.
7. the seed dormant method of quick releasing Java brucea according to claim 6, it is characterized in that: by described step 5) in Java brucea seed after dipping proceed in the illumination box of constant temperature or temperature match curing conditions, mix in the sand of 30 % by weight～40 % by weight coconut palm chaffs and germinate.
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Cited By (5)
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| CN104541661A (en) * | 2014-12-25 | 2015-04-29 | 肇庆学院 | Germination accelerating and seedlings growing method of bauhinia championii seed peace beans |
| CN105284534A (en) * | 2015-11-03 | 2016-02-03 | 大新县生产力促进中心 | Planting method for rucea javanica |
| CN107820776A (en) * | 2017-11-14 | 2018-03-23 | 广西壮族自治区药用植物园 | The method for promoting Java brucea seed to sprout |
| CN108243657A (en) * | 2018-02-23 | 2018-07-06 | 广西师范学院 | The method of Rapid reversal mountain fourth seed dormancy |
| CN109588226A (en) * | 2019-01-25 | 2019-04-09 | 河北省农林科学院石家庄果树研究所 | A method of improving strawberry hybrid seed germination percentage and Phase ofsprouting |
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| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
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| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104541661A (en) * | 2014-12-25 | 2015-04-29 | 肇庆学院 | Germination accelerating and seedlings growing method of bauhinia championii seed peace beans |
| CN104541661B (en) * | 2014-12-25 | 2016-06-29 | 肇庆学院 | The accelerating germination of Caulis Bauhihiae Championii seed safety bean and method for culturing seedlings |
| CN105284534A (en) * | 2015-11-03 | 2016-02-03 | 大新县生产力促进中心 | Planting method for rucea javanica |
| CN107820776A (en) * | 2017-11-14 | 2018-03-23 | 广西壮族自治区药用植物园 | The method for promoting Java brucea seed to sprout |
| CN108243657A (en) * | 2018-02-23 | 2018-07-06 | 广西师范学院 | The method of Rapid reversal mountain fourth seed dormancy |
| CN109588226A (en) * | 2019-01-25 | 2019-04-09 | 河北省农林科学院石家庄果树研究所 | A method of improving strawberry hybrid seed germination percentage and Phase ofsprouting |
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| CN104106327B (en) | 2016-08-17 |
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