CN107820776A - The method for promoting Java brucea seed to sprout - Google Patents
The method for promoting Java brucea seed to sprout Download PDFInfo
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- CN107820776A CN107820776A CN201711123769.2A CN201711123769A CN107820776A CN 107820776 A CN107820776 A CN 107820776A CN 201711123769 A CN201711123769 A CN 201711123769A CN 107820776 A CN107820776 A CN 107820776A
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- seed
- java brucea
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Promote the method that Java brucea seed is sprouted comprising following steps the invention discloses a kind of：Step 1, Java brucea seed is taken, put it in thimerosal, placed 9~10 minutes, taken out seed and be put into the trisodium phosphate solution that mass concentration is 11~21%, place 9~10 minutes, take out seed and cleaned with water, obtain seed I；Step 2, the seed I is soaked in the mixed solution containing kinetin and gibberellin 36~40 hours, obtain seed II, gained seed II is cultivated 30~35 days and sprouted, wherein, concentration of the kinetin in mixed solution is 30~35mg/L, and concentration of the gibberellin in mixed solution is 100~300mg/L.This method effectively raises the breeding potential of seed, reduces the utilization to wild resource.
Description
Technical field
The present invention relates to plant seed germination technical field, more particularly to a kind of method for promoting Java brucea seed to sprout.
Background technology
Java brucea, also known as crow courage, crow courage, bitter fibert etc., it is Simarubaceae, brucea plant evergreen shrubs or little Qiao
Wood, it is distributed in the provinces and regions such as Fujian, Taiwan, Guangdong, Guangxi, Hainan and Yunnan；Java brucea has many application values, especially
It is in medical applications, there is the effects such as heat-clearing and damp-drying drug, removing toxic substances desinsection, corrosion, while Java brucea has antitumor, anti-alimentary tract
The pharmacological actions such as ulcer, reducing blood lipid, anti-inflammation.At present, the research both at home and abroad to Java brucea focuses primarily upon constituent analysis, medicine
Reason effect, tissue culture propagation etc., and seminal propagation has no report.Java brucea is evergreen shrubs or dungarunga, is born in height above sea level
In 950-1000m lime mountain sparse woods.It is medicinal mainly based on wild resource at present, and the blindness of wild resource is gathered, gesture
The destruction of ecological environment and in imminent danger, and the difference of ecological environment of wild resource must be caused, makes to introduce a fine variety and tames highly difficult.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least following by the excellent of explanation
Point.
It is a further object of the invention to provide a kind of method for promoting Java brucea seed to sprout, seed is effectively increased
Germination rate.
Adopted the following technical scheme that to achieve the above object with some other purposes, the present invention：
It is a kind of to promote the method that Java brucea seed is sprouted comprising following steps：
Step 1, Java brucea seed is taken, put it in thimerosal, placed 9~10 minutes, it is dense that taking-up seed is put into quality
Spend in the trisodium phosphate solution for 11~21%, place 9~10 minutes, take out seed and cleaned with water, obtain seed I；
Step 2, the seed I is soaked in the mixed solution containing kinetin and gibberellin 36~40 hours, obtained
Seed II, by gained seed II cultivate 30~35 days i.e. sprout, wherein, concentration of the kinetin in mixed solution be 30~
35mg/L, concentration of the gibberellin in mixed solution are 100~300mg/L.
Preferably, in the described method for promoting the sprouting of Java brucea seed, in step 1, the Java brucea seed is existed
Low temperature wet storage 3-7 weeks is carried out under conditions of 5-7 DEG C of temperature, relative humidity 20-30%.
Preferably, in the described method for promoting the sprouting of Java brucea seed, in step 1, the thimerosal includes quality
The ethanol solution that the Eusol or mass concentration that concentration is 0.6~0.7% are 80~90%.
Preferably, in the described method for promoting the sprouting of Java brucea seed, the thimerosal is including mass concentration
0.5% Eusol or mass concentration is 85% ethanol solution.
Preferably, in the described method for promoting the sprouting of Java brucea seed, in step 1, Java brucea seed is taken, is put
Into thimerosal, place 10 minutes, take out seed and be put into the trisodium phosphate solution that mass concentration is 15%, place 10 minutes,
Take out seed and cleaned with water, obtain seed I.
Preferably, in the described method for promoting the sprouting of Java brucea seed, mixed solution also contains described in step 2：
Nucleotides and compound sodium nitrophenolate, wherein, concentration of the nucleotides in mixed solution is 30-40mg/L, compound sodium nitrophenolate in mixed solution
In concentration be 5-6mg/L.
Preferably, in the described method for promoting the sprouting of Java brucea seed, in step 2, the seed I is soaked in and contained
Have in the mixed solution of kinetin and gibberellin 38 hours, obtain seed II, gained seed II is cultivated 31 days and sprouted, its
In, concentration of the kinetin in mixed solution is 30mg/L, and concentration of the gibberellin in mixed solution is 240mg/L.
Preferably, in the described method for promoting the sprouting of Java brucea seed, in step 2, condition of culture is that temperature is 20
~32 DEG C, relative air humidity is 75~85%.
Preferably, in the described method for promoting the sprouting of Java brucea seed, condition of culture is that temperature is 30 DEG C, air phase
It is 83% to humidity.
The present invention comprises at least following beneficial effect：The present invention is by providing a kind of side for promoting Java brucea seed to sprout
Method, the mixed solution for the gibberellin for being 100~300mg/L the kinetin and mass concentration that mass concentration is 30~35mg/L,
Java brucea seed is soaked, the breeding potential of seed has been brought up to more than 80%, reduces the utilization to wild resource, quick increase
Its breeding coefficient, while generation the problems such as avoid decline, variation, be advantageous to keep the merit of seedling, improve kind
The security that seedling uses, technical support is provided to resources of medicinal plant regeneration in short supply in imminent danger and sustainable utilization.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
The present invention is elaborated below, to make those of ordinary skill in the art can be real according to this after referring to this specification
Apply.
It is a kind of to promote the method that Java brucea seed is sprouted comprising following steps：Step 1, Java brucea seed is taken, will
It is put into thimerosal, is placed 9~10 minutes, is taken out seed and is put into the trisodium phosphate solution that mass concentration is 11~21%,
Place 9~10 minutes, take out seed and cleaned with water, obtain seed I；Step 2, the seed I is soaked in containing kinetin and
36~40 hours in the mixed solution of gibberellin, seed II is obtained, gained seed II is cultivated 30~35 days and sprouted, wherein,
Concentration of the kinetin in mixed solution is 30~35mg/L, concentration of the gibberellin in mixed solution is 100~
300mg/L, the breeding potential of seed has been brought up to more than 80%, reduce the utilization to wild resource, it is numerous quickly to add its
The generation for the problems such as growing coefficient, while avoiding decline, variation, be advantageous to keep the merit of seedling, improve seedling and use
Security, provide technical support to resources of medicinal plant in short supply regeneration in imminent danger and sustainable utilization.
In the described method for promoting Java brucea seed to sprout, in step 1, by the Java brucea seed 5-7 DEG C of temperature,
Low temperature wet storage 3-7 weeks is carried out under conditions of relative humidity 20-30%.
Described promotes in the method that Java brucea seed is sprouted, and in step 1, it is 0.6 that the thimerosal, which includes mass concentration,
~0.7% Eusol or mass concentration is 80~90% ethanol solution.
Described promotes in the method that Java brucea seed is sprouted, and the thimerosal includes the bleaching that mass concentration is 0.5%
Powder solution or the ethanol solution that mass concentration is 85%.
Described promotes in the method that Java brucea seed is sprouted, and in step 1, takes Java brucea seed, puts it to thimerosal
In, place 10 minutes, take out seed and be put into the trisodium phosphate solution that mass concentration is 15%, place 10 minutes, take out seed
And cleaned with water, obtain seed I.
Described promotes in the method that Java brucea seed is sprouted, and mixed solution also contains described in step 2：Nucleotides and multiple
Nitre phenol sodium, wherein, concentration of the nucleotides in mixed solution is 30-40mg/L, concentration of the compound sodium nitrophenolate in mixed solution is
5-6mg/L。
Described promotes in the method that Java brucea seed is sprouted, and in step 2, the seed I is soaked in containing kinetin
With 38 hours in the mixed solution of gibberellin, obtain seed II, by gained seed II cultivate 31 days i.e. sprout, wherein, it is described swash
Concentration of the therbligs in mixed solution is 30mg/L, and concentration of the gibberellin in mixed solution is 240mg/L.
Described promotes in the method that Java brucea seed is sprouted, and in step 2, condition of culture is that temperature is 20~32 DEG C, empty
Gas relative humidity is 75~85%.
Described promotes in the method that Java brucea seed is sprouted, and condition of culture is that temperature is 30 DEG C, and relative air humidity is
83%.
Described promotes in the method that Java brucea seed is sprouted, and chooses the not damaged, full seed, matter of black ripening fruits
Ground is uniform, does not carry the seed progress rudiment culture of worm's ovum.
Embodiment 1
1st, stage of ripeness fruit is harvested, pericarp is peelled off, washes away pulp, not damaged, full seed, matter are therefrom chosen after drying in the shade
Ground is uniform, does not carry 100 Java brucea seeds of disease pest ovum.
2nd, Java brucea seed is put into the Eusol that mass concentration is 0.6%, placed 10 minutes, taken out seed and put
In the trisodium phosphate solution for being 11% to mass concentration, place 9 minutes, take out seed and cleaned 3 times with water, obtain seed I；
3rd, by the seed I, it is put into the mixed solution containing kinetin and gibberellin, wherein kinetin is in mixed solution
In concentration be 30mg/L, concentration of the gibberellin in mixed solution be 100mg/L, place 36 hours, take out seed then exist
Temperature be 20 DEG C, relative humidity be 75% under conditions of cultivate 30 days first group obtain 80 germination seeds.
Embodiment 2
1st, stage of ripeness fruit is harvested, pericarp is peelled off, washes away pulp, not damaged, full seed, matter are therefrom chosen after drying in the shade
Ground is uniform, does not carry 100 Java brucea seeds of disease pest ovum.
2nd, Java brucea seed is put into the ethanol solution that mass concentration is 85%, placed 10 minutes, taken out seed and be put into
Mass concentration is in 18% trisodium phosphate solution, is placed 10 minutes, takes out seed and is cleaned 4 times with water, obtains seed I.
3rd, by the seed I, it is put into the mixed solution containing kinetin and gibberellin, wherein kinetin is in mixed solution
In concentration be 32mg/L, concentration of the gibberellin in mixed solution be 180mg/L, place 38 hours, take out seed then exist
Temperature be 25 DEG C, relative humidity be 80% under conditions of cultivate 32 days first group obtain 83 germination seeds.
Embodiment 3
1st, stage of ripeness fruit is harvested, pericarp is peelled off, washes away pulp, not damaged, full seed, matter are therefrom chosen after drying in the shade
Ground is uniform, does not carry 100 Java brucea seeds of disease pest ovum.
2nd, it is 5 DEG C in temperature by Java brucea seed, under conditions of relative humidity is 20%, low temperature wet storage 5 weeks.
3rd, the Java brucea seed after low temperature wet storage is put into the ethanol solution that mass concentration is 90%, placed 10 minutes,
Take out seed to be put into the trisodium phosphate solution that mass concentration is 21%, place 10 minutes, take out seed and cleaned 4 times with water, obtained
To seed I.
4th, by the seed I, it is put into the mixed solution containing kinetin and gibberellin, wherein kinetin is in mixed solution
In concentration be 35mg/L, concentration of the gibberellin in mixed solution be 300mg/L, place 40 hours, take out seed then exist
Temperature be 32 DEG C, relative humidity be 85% under conditions of cultivate 35 days first group obtain 86 germination seeds.
Comparative example 1
1st, stage of ripeness fruit is harvested, pericarp is peelled off, washes away pulp, not damaged, full seed, matter are therefrom chosen after drying in the shade
Ground is uniform, does not carry 100 Java brucea seeds of disease pest ovum.
2nd, it is 5 DEG C in temperature by Java brucea seed, under conditions of relative humidity is 20%, low temperature wet storage 5 weeks.
3rd, the Java brucea seed after low temperature wet storage is put into the ethanol solution that mass concentration is 90%, placed 10 minutes,
Take out seed to be put into the trisodium phosphate solution that mass concentration is 21%, place 10 minutes, take out seed and cleaned 4 times with water, obtained
To seed I.
4th, by the seed I, it is put into distilled water, places 40 hours, then it is 32 DEG C in temperature to take out seed, relatively wet
Spend and obtain 3 germination seeds to cultivate 35 days under conditions of 85%.
Comparative example 2
1st, stage of ripeness fruit is harvested, pericarp is peelled off, washes away pulp, not damaged, full seed, matter are therefrom chosen after drying in the shade
Ground is uniform, does not carry 100 Java brucea seeds of disease pest ovum.
2nd, it is 5 DEG C in temperature by Java brucea seed, under conditions of relative humidity is 20%, low temperature wet storage 5 weeks.
3rd, the Java brucea seed after low temperature wet storage is put into the ethanol solution that mass concentration is 90%, placed 10 minutes,
Take out seed to be put into the trisodium phosphate solution that mass concentration is 21%, place 10 minutes, take out seed and cleaned 4 times with water, obtained
To seed I.
4th, by the seed I, it is put into the Gibberellins solution that concentration is 80-120mg/ml, places 40 hours, take out seed
Then it is 32 DEG C in temperature, relative humidity is cultivated 35 days under conditions of being 85% and obtains 13 germination seeds.
The seed of table 1 sprouts the table of comparisons
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | |
| Seed amount | 100 | 100 | 100 | 100 | 100 |
| Sprout quantity | 80 | 83 | 86 | 3 | 13 |
| Germination rate | 80% | 83% | 86% | 3% | 13% |
As shown in table 1, the seed germination rate of embodiment 1, embodiment 2 and embodiment 3 is up to more than 80%, and comparative example 1
Seed germination rate with comparative example 2 is only 3% and 13%, illustrates that this method makes the breeding potential of seed bring up to more than 80%,
Its breeding coefficient is quickly added, reduces the utilization to wild resource, resources of medicinal plant in short supply in imminent danger is regenerated and continued
Using providing technical support.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details.
Claims (9)
1. a kind of promote the method that Java brucea seed is sprouted comprising following steps：
Step 1, Java brucea seed is taken, put it in thimerosal, placed 9~10 minutes, taking-up seed is put into mass concentration and is
In 11~21% trisodium phosphate solution, place 9~10 minutes, take out seed and cleaned with water, obtain seed I；
Step 2, the seed I is soaked in the mixed solution containing kinetin and gibberellin 36~40 hours, obtains seed
II, by gained seed II cultivate 30~35 days i.e. sprout, wherein, concentration of the kinetin in mixed solution be 30~
35mg/L, concentration of the gibberellin in mixed solution are 100~300mg/L.
2. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, in step 1, by the Java brucea seed
Low temperature wet storage 3-7 weeks is carried out under conditions of 5-7 DEG C of temperature, relative humidity 20-30%.
3. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, in step 1, the thimerosal includes matter
Measure the Eusol that concentration is 0.6~0.7% or the ethanol solution that mass concentration is 80~90%.
4. the method for promoting Java brucea seed to sprout as claimed in claim 2, wherein, the thimerosal is including mass concentration
0.5% Eusol or mass concentration is 85% ethanol solution.
5. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, in step 1, Java brucea seed is taken, will
It is put into thimerosal, is placed 10 minutes, is taken out seed and is put into the trisodium phosphate solution that mass concentration is 15%, places 10 points
Clock, take out seed and cleaned with water, obtain seed I.
6. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, mixed solution also contains described in step 2
Have：Nucleotides and compound sodium nitrophenolate, wherein, concentration of the nucleotides in mixed solution is 30-40mg/L, compound sodium nitrophenolate is molten in mixing
Concentration in liquid is 5-6mg/L.
7. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, in step 2, the seed I is soaked
38 hours in the mixed solution containing kinetin and gibberellin, seed II is obtained, gained seed II is cultivated 31 days and sprouted,
Wherein, concentration of the kinetin in mixed solution is 30mg/L, and concentration of the gibberellin in mixed solution is 240mg/L.
8. the method for promoting Java brucea seed to sprout as claimed in claim 1, wherein, in step 2, condition of culture is that temperature is
20~32 DEG C, relative air humidity is 75~85%.
9. the method for promoting Java brucea seed to sprout as claimed in claim 7, wherein, condition of culture is that temperature is 30 DEG C, empty
Gas relative humidity is 83%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711123769.2A CN107820776A (en) | 2017-11-14 | 2017-11-14 | The method for promoting Java brucea seed to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711123769.2A CN107820776A (en) | 2017-11-14 | 2017-11-14 | The method for promoting Java brucea seed to sprout |
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| CN107820776A true CN107820776A (en) | 2018-03-23 |
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| CN201711123769.2A Pending CN107820776A (en) | 2017-11-14 | 2017-11-14 | The method for promoting Java brucea seed to sprout |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106327A (en) * | 2014-06-20 | 2014-10-22 | 姚俊华 | Method for rapidly releasing dormancy of brucea javanica seeds |
| CN105284534A (en) * | 2015-11-03 | 2016-02-03 | 大新县生产力促进中心 | Planting method for rucea javanica |
-
2017
- 2017-11-14 CN CN201711123769.2A patent/CN107820776A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106327A (en) * | 2014-06-20 | 2014-10-22 | 姚俊华 | Method for rapidly releasing dormancy of brucea javanica seeds |
| CN105284534A (en) * | 2015-11-03 | 2016-02-03 | 大新县生产力促进中心 | Planting method for rucea javanica |
Non-Patent Citations (1)
| Title |
|---|
| 陈瑛 主编: "《实用中药种子技术手册》", 30 June 1999, 人民卫生出版社 * |
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