CN103518608B - Anoectochilus formosanus seedling cultivation medium and seedling cultivation method - Google Patents

Anoectochilus formosanus seedling cultivation medium and seedling cultivation method Download PDF

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CN103518608B
CN103518608B CN201310503926.8A CN201310503926A CN103518608B CN 103518608 B CN103518608 B CN 103518608B CN 201310503926 A CN201310503926 A CN 201310503926A CN 103518608 B CN103518608 B CN 103518608B
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cultivation
seedling
terminal bud
roxburgh anoectochilus
anoectochilus terminal
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CN103518608A (en
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黄永辉
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Xianyou County Sen Wen Agricultural Development Co Ltd
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Xianyou County Sen Wen Agricultural Development Co Ltd
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Abstract

The invention provides an anoectochilus formosanus seedling cultivation medium and a seedling cultivation method. The medium mainly comprises components of peanut shells and rosewood saw dust, wherein the peanut shells and the rosewood saw dust are 3-5:1 by the weight ratio, the peanut shells and the rosewood saw dust are mixed at the weight ratio 3-5:1 and are sufficiently steeped with water, anaerobic fermentation is carried out for 10-20 days, and the medium is prepared by spraying nanometer bamboo vinegar diluent which can sufficiently wet the components, wherein the nanometer bamboo vinegar diluent is obtained by diluting original fluid with water for 200-400 times. The nanometer bamboo vinegar diluent replaces carbendazim or other farm chemicals for sterilizing and preventing insect diseases in cultivation. Cultivation under forests enables the growing environment of anoectochilus formosanus to tend to be more natural, the quality of the anoectochilus formosanus is made to tend to be wild, the cultivation under the forecasts can also save the cost of traditional cultivation shed building and land cost, and the space of the forests is effectively utilized.

Description

Roxburgh anoectochilus terminal bud seedling cultivation matrix and seedling cultivation method
Technical field
The present invention relates to a kind of cultivation matrix of roxburgh anoectochilus terminal bud, relate to the cultivation matrix of a kind of roxburgh anoectochilus terminal bud seedling particularly.
Background technology
The wild rare medicinal plant that roxburgh anoectochilus terminal bud (Anoectochilus roxburghii) is the orchid family Anoectochilus Blume." Fujian medicine will " carries: its nature and flavor are flat, sweet, there is refrigerant blood, expelling wind and removing dampness effect, cure mainly spitting of blood, bronchitis, ephritis, cystitis, diabetes, wind arthritis, acute infantile convulsion wind, venomous snake bite etc., be among the peoplely used for children's's high fever, infantile convulsion.In recent years, roxburgh anoectochilus terminal bud, in the difficult and complicated illness such as treatment hypertension, diabetes and tumour, causes the attention of the world of medicine day by day.。Roxburgh anoectochilus terminal bud is mainly distributed in China's southeastern coast and south east asia, and its sales volume, the market price are in the trend risen year by year.Because it requires higher to growing environment, add long-term excessively the gathering of people, its natural resources is in day by day exhausted trend; in Fujian Province; roxburgh anoectochilus terminal bud has been put into Fujian Province's Endangered Medicinal Herb and has been protected, and therefore, is badly in need of the artificial cultivation technique improving roxburgh anoectochilus terminal bud.
Disclose a kind of culture technique of roxburgh anoectochilus terminal bud seedling in CN100407897C, its facility comprises the seedling disk frame of cultivating chamber and indoor ordered arrangement, seedling dish, and seedling dish is placed on seedling disk frame; Cultivating chamber can be Based Intelligent Control or shading screen canopy, and seedling disk frame is the structure of 1 to multilayer, and its specifications and standards seedling dish adapts; Cultivation matrix is hypnum moss or liver moss, and plantlet in vitro, after cleaning, sterilization, is transplanted in the matrix in seedling disk point, then rationally controls after the wet and temperature control of light, water spray, fertilising, control cultivates a period of time, results roxburgh anoectochilus terminal bud finished product.The method is adapted to the cultivation of intensive scale or scattered cultivation, and have production cost low, output is high, implements the feature that is easy to.But, cultivating in the process of roxburgh anoectochilus terminal bud according to said method, need at interval of spraying a leaf fertilizer (0.3% urea+0.1% potassium dihydrogen phosphate) when 5 ~ 7 days, at interval of watering a fertilizer (mixing of soybean leachate+0.3% is fertile) when 10 ~ 15 days, not only operate more loaded down with trivial details, and easily introduce the extraneous contamination destruction roxburgh anoectochilus terminal bud sterile environment required when transplanting in operation (for improving survival rate, roxburgh anoectochilus terminal bud seedling should be noted the sterilization of soil and the sterilization of plant itself when cultivating, usual use carbendazim or disinfecting solution of potassium permanganate), so that there is roxburgh anoectochilus terminal bud damping off, the survival rate of seedling and seedlings is caused to reduce, in addition, the method utilizes hypnum moss or liver moss as the medium of roxburgh anoectochilus terminal bud, easily causes vegetation deterioration, causes soil desertification.
Summary of the invention
The object of the present invention is to provide a kind of roxburgh anoectochilus terminal bud seedling cultivation matrix, to solve the above-mentioned problems in the prior art.The present invention, by using the cultivation matrix of new formula, makes roxburgh anoectochilus terminal bud can without the need to imposing nutritious fertilizer in a growth cycle, only needs dailyly to heat, illumination etc., and survival rate is up to more than 95%.
Another object of the present invention is to provide the cultivation method of a kind of roxburgh anoectochilus terminal bud seedling.
Technical scheme provided by the invention is as follows:
Roxburgh anoectochilus terminal bud seedling cultivation matrix, it is by the component mainly comprising following weight: peanut shell mixes with the ratio of weight ratio 3-5:1 with redwood sawdust, fully soak with water after, through the anaerobic fermentation of 10 ~ 20 days, spray preparation with the nanometer bamboo vinegar dilution being enough to wetting above-mentioned component again, described nanometer bamboo vinegar dilution is stoste dilute with water 200-400 times.
In August, 2000, the redwood standard that first, China is legal---" redwood " national standard is is formally issued and implemented, and expressly provides in standard, redwood is divided into 5 genus 8 class 33 kinds.5 genus are what to name with the genus of dendrology, and namely Pterocarpus, Dalbergia, precipice Macroptilium and ferreous mesua belong to.8 classes are then name with the trade name of timber, i.e. narra class, rosewood class, fragrant branch wood class, nigrotic acid branch class, red acid branch wood class, ebony class, striped ebony class and Door frame class.Redwood sawdust of the present invention produces dead meal--redwood sawdust when being processing furniture.Redwood sawdust of the present invention can be the redwood sawdust of multiclass mixing.Mixed proportion is not limit.Redwood sawdust of the present invention is preferably the arbitrary proportion mixture of rose wood class and Door frame class.Because its source is wider.
Another technical scheme of the present invention is:
A cultivation method for roxburgh anoectochilus terminal bud seedling, comprises the following steps:
1) seedling selected to adapt to extraneous environment, controls indoor illumination intensity at 1000 ~ 1800Lx, light application time 8 ~ 10 hours/day indoor cultivation 10-15 days, relative air humidity is 65 ~ 85%, indoor temperature, at 20-26 DEG C, keeps room ventilation, controls cultivation matrix moistening simultaneously;
2) first enter seedling strain with nanometer bamboo vinegar dilution and carry out sterilization, then gold thread is placed in nanometer bamboo vinegar dilution to soak and can fills basket in 1-2 minute; Dress basket after seedling put down again tree cultivation under woods ' utilize the woods shelter from heat or light control temp. and humidity, cache-sex must be spread in case direct sunlight on trees when just putting basket, and control temperature is at 26 ± 3 DEG C, humidity is at 60%-80%, and luminosity sprays nanometer bamboo vinegar dilution and replaces agricultural chemicals smelting insect pest during 1000 to 2000lux. cultivations;
3) results roxburgh anoectochilus terminal bud finished product is cultured under these conditions.
In the preferred embodiment, described roxburgh anoectochilus terminal bud seedling is be highly the seedling of 5 ~ 7cm.Shoot survival percent within the scope of this is the highest.
In the preferred embodiment, described nanometer bamboo vinegar dilution is stoste dilute with water 200-400 times.
The present invention uses peanut shell and redwood sawdust as the raw material of roxburgh anoectochilus terminal bud seedling cultivation matrix, peanut shell food is gathered or is processed the waste material of generation, and the waste material produced when redwood sawdust is processing mahogany furniture, owing to being waste material, above-mentioned material is not only with low cost, and easily obtains.
Bamboo vinegar is in the process of making charcoal with bamboo wood, collects the gas that bamboo wood produces in pyrolytic, and this gas is cooled at normal temperatures the liquid substance obtained.Bamboo vinegar contains nearly 300 kinds of natural polymer organic compounds, has the alkaline components etc. of organic acid, alcohols, ketone, aldehydes, ester class and trace.
Different from existing cultivation matrix disinfection way, cultivation matrix of the present invention, after the anaerobic fermentation of 10 ~ 20 days, can kill the worm's ovum and aerobic bacteria that contain in base starting material substantially; In addition, because matrix disinfecting process does not use agricultural chemicals to carry out disinfection to matrix, also can reduce the residue of pesticide in cultivation matrix, and then reduce the residue of pesticide in medicinal material.
Cultivation matrix of the present invention can provide the nutrient needed for the growth of roxburgh anoectochilus terminal bud seedling, and, owing to being the mixture of peanut shell and redwood sawdust, its porosity is high, make roxburgh anoectochilus terminal bud in a growth cycle without the need to imposing any fertilizer, and substantially get rid of the interference of the damage by disease and insect caused by matrix, improve the survival rate after roxburgh anoectochilus terminal bud sprigging, reduce the incidence of roxburgh anoectochilus terminal bud damping off.
Roxburgh anoectochilus terminal bud finished product described in the present invention refers to the fresh grass that can go on the market.
Add each Plant Hormone during roxburgh anoectochilus terminal bud tissue culture, even have people also to add when planting and spray appropriate plant hormone for growth promoting effects; Meanwhile, roxburgh anoectochilus terminal bud is high to environmental requirement, and damage by disease and insect is heavier, and how when not using chemical pesticide, disease and pest control is the key technology difficult problem needing in organic production to solve.Plant hormone and the agricultural chemicals residual worry gradually becoming people in roxburgh anoectochilus terminal bud finished product.The present invention adds nontoxic pure natural nanometer bamboo vinegar in matrix, and periodically sprinkle nanometer bamboo vinegar dilution again in planting process, can promote that roxburgh anoectochilus terminal bud grows, again can prevention and elimination of disease and pests, and health environment-friendly.
Utilize the peanut shell fermentation substrate together with the nanometer bamboo vinegar of redwood sawdust pulverized, not only can obtain a kind of soil-free organic matrixes being applicable to the growth of prosperous line lotus newly, and effectively can make use of a large amount of discarded peanut shells and redwood sawdust, the modern nanometer bamboo vinegar of first Application adds in matrix and instead of plant hormone and agricultural chemicals for Promoting plant growth and pest control, improves the quality of roxburgh anoectochilus terminal bud.In cultivation, replace how clever bacterium or other agricultural chemicals to have prevention and elimination of disease and pests for sterilization.Cultivation under woods can allow roxburgh anoectochilus terminal bud be more prone to nature at growing environment, allows its quality more to wild, and cultivation under woods can save cost and the cost of land of traditional cultivation scaffolding, effectively make use of woods space,
Embodiment
Embodiment 1
Raw material sources: peanut shell is the agricultural wastes of local purchase
The discarded object of redwood sawdust for purchasing from local mahogany furniture factory, it is the mixture of multiple types redwood.
Bamboo vinegar is bought by business and is obtained.
The making of culture matrix: peanut shell crushes, mix by weight 4:1 with redwood sawdust, through water soaking 7 days, drench to allow peanut shell and redwood sawdust, build heap afterwards, carry out anaerobic fermentation 10 days with plastic film seal, then with the nanometer bamboo vinegar of dilution 200 times peanut shell and redwood sawdust piled and spray, make it by nanometer bamboo vinegar complete wetting, can basket be filled.Each indigo plant is long is 26cm, and wide is 35cm, and it is thick that the cultivation matrix in basket can lay about 3-5cm.
Indoor growing: the seedling selected, height of seedling is at 5-7 centimetre, indoor cultivation 10 to 15 days to adapt to extraneous environment, first enter seedling strain with nanometer bamboo vinegar 300 times of dilutions and carry out sterilization, then gold thread is placed in nanometer bamboo vinegar 300 times of dilutions to soak and can fills basket in 1-2 minute.The planting density of roxburgh anoectochilus terminal bud is that each basket pot trains 130, control indoor temperature and remain on 20-26 degree, indoor illumination intensity is at 1000 ~ 1800Lx, light application time 8 ~ 10 hours/day, relative air humidity is 65 ~ 85%, keep room ventilation, periodically sprinkle nanometer bamboo vinegar 300 times of dilutions control cultivation matrix simultaneously, keep moistening;
Cultivation under woods: the seedling after dress basket puts down tree cultivation under woods again, utilize the woods shelter from heat or light control temp. and humidity, cache-sex must be spread in case direct sunlight on trees when just putting basket, and control temperature is at 26 ± 3 DEG C, humidity is at 60%-80%, luminosity is at 1000.Lux to 2000.Lux, and the nanometer bamboo vinegar dilution spraying 250 times during cultivation replaces agricultural chemicals (as how clever bacterium) smelting insect pest.
In underwood planting after 4 months, results roxburgh anoectochilus terminal bud finished product, the fresh careless 2.1g of every strain average weight, average height is 15-20cm.Its survival rate is more than 95%.
Embodiment 2
Raw material sources: peanut shell is the agricultural wastes of local purchase
The discarded object of redwood sawdust for purchasing from local mahogany furniture factory, it is the mixture of multiple types redwood.
Bamboo vinegar is bought by business and is obtained.
The making of culture matrix: peanut shell crushes and mixes by weight 4:1 with aforementioned redwood sawdust, through water soaking 7 days, drench to allow peanut shell and redwood sawdust, build heap afterwards, anaerobic fermentation is carried out 20 days with plastic film seal, with the nanometer bamboo vinegar of dilution 300 times peanut shell and redwood sawdust piled again and spray, make it by nanometer bamboo vinegar complete wetting, can basket be filled.Each indigo plant is long is 28cm, and wide is 45cm, and it is thick that the cultivation matrix in basket can lay about 3-5cm.
Indoor growing: the seedling selected, height of seedling is at 5-7 centimetre, indoor cultivation 10 to 15 days to adapt to extraneous environment, first enter seedling strain with nanometer bamboo vinegar 300 times of dilutions and carry out sterilization, then gold thread is placed in nanometer bamboo vinegar 300 times of dilutions to soak and can fills basket in 1-2 minute.The planting density of roxburgh anoectochilus terminal bud is that each basket pot trains 130, control indoor temperature and remain on 20-26 degree, indoor illumination intensity is at 1000 ~ 1800Lx, light application time 8 ~ 10 hours/day, relative air humidity is 65 ~ 85%, keep room ventilation, periodically sprinkle nanometer bamboo vinegar 300 times of dilutions control cultivation matrix simultaneously, keep moistening;
Cultivation under woods: the seedling after dress basket puts down tree cultivation under woods again, utilize the woods shelter from heat or light control temp. and humidity, cache-sex must be spread in case direct sunlight on trees when just putting basket, and control temperature is at 26 ± 3 DEG C, humidity is at 60%-80%, luminosity is 1000 to 2000.Lux, and the nanometer bamboo vinegar dilution spraying 250 times during cultivation replaces agricultural chemicals smelting insect pest.
In underwood planting after 4 months, results roxburgh anoectochilus terminal bud finished product, the fresh careless 2g of every strain average weight, average height is 15-20cm.Its survival rate is 96%.
Embodiment 3
Raw material sources: peanut shell is the agricultural wastes of local purchase
The discarded object of redwood sawdust for purchasing from local mahogany furniture factory, is mainly the mixture of rose wood class and Door frame class redwood.
Bamboo vinegar is bought by business and is obtained.
The making of culture matrix: peanut hull meal is broken to (coarse crushing), mix by weight 4:1 with sawdust, through water soaking 7 days, drench to allow peanut shell and redwood sawdust, build heap afterwards, carry out anaerobic fermentation 15 days with plastic film seal, then with the nanometer bamboo vinegar of dilution 250 times peanut shell and redwood sawdust piled and spray, make it by nanometer bamboo vinegar complete wetting, can basket be filled.Each basket is long is 28cm, and wide is 45cm, and it is thick that the cultivation matrix in basket can lay about 3-5cm.
Indoor growing: the seedling selected, height of seedling is at 5-7 centimetre, indoor cultivation 10 to 15 days to adapt to extraneous environment, first enter seedling strain with nanometer bamboo vinegar 300 times of dilutions and carry out sterilization, then gold thread is placed in nanometer bamboo vinegar 300 times of dilutions to soak and can fills basket in 1-2 minute.The planting density of roxburgh anoectochilus terminal bud is that each basket pot trains 125, control indoor temperature and remain on 20-26 degree, indoor illumination intensity is at 1000 ~ 1800Lx, light application time 8 ~ 10 hours/day, relative air humidity is 65 ~ 85%, keep room ventilation, periodically sprinkle nanometer bamboo vinegar 300 times of dilutions control cultivation matrix simultaneously, keep moistening;
Cultivation under woods: the seedling after dress basket puts down tree cultivation under woods again, utilize the woods shelter from heat or light control temp. and humidity, cache-sex must be spread in case direct sunlight on trees when just putting basket, and control temperature is at 26 ± 3 DEG C, humidity is at 60%-80%, luminosity is 1000 to 2000.Lux, and the nanometer bamboo vinegar dilution spraying 250 times during cultivation replaces agricultural chemicals smelting insect pest.
In underwood planting after 4 months, results roxburgh anoectochilus terminal bud finished product, the fresh careless 2.05g of every strain average weight, average height is 15-20cm.Its survival rate is 95%.

Claims (6)

1. roxburgh anoectochilus terminal bud seedling cultivation matrix, it is by the component mainly comprising following weight: peanut shell mixes with the ratio of weight ratio 3-5:1 with redwood sawdust, fully soak with water after, through the anaerobic fermentation of 10 ~ 20 days, spray preparation with the nanometer bamboo vinegar dilution being enough to wetting above-mentioned component again, described nanometer bamboo vinegar dilution is stoste dilute with water 200-400 times.
2. according to the roxburgh anoectochilus terminal bud seedling cultivation matrix described in claim 1, it is characterized in that: described redwood comprises the arbitrary proportion mixture of the redwood of any kind.
3. according to the roxburgh anoectochilus terminal bud seedling cultivation matrix described in claim 1 or 2, it is characterized in that: described redwood is the arbitrary proportion mixture of rose wood class and Door frame class.
4. a cultivation method for roxburgh anoectochilus terminal bud seedling, comprises the following steps:
1) seedling selected to adapt to extraneous environment, controls indoor illumination intensity at 1000 ~ 1800Lx, light application time 8 ~ 10 hours/day indoor cultivation 10-15 days, relative air humidity is 65 ~ 85%, indoor temperature, at 20-26 DEG C, keeps room ventilation, controls cultivation matrix moistening simultaneously; Described matrix is matrix prepared by claim 1;
2) first enter seedling strain with nanometer bamboo vinegar dilution and carry out sterilization, then roxburgh anoectochilus terminal bud is placed in nanometer bamboo vinegar dilution to soak and can fills basket in 1-2 minute; Seedling after dress basket puts down tree cultivation under woods again, utilize the woods shelter from heat or light control temp. and humidity, cache-sex must be spread in case direct sunlight on trees when just putting basket, and control temperature is at 26 ± 3 DEG C, humidity is at 60%-80%, and luminosity sprays nanometer bamboo vinegar dilution and replaces agricultural chemicals smelting insect pest during 1000 to 2000lux. cultivations;
3) results roxburgh anoectochilus terminal bud finished product is cultured under these conditions.
5. the cultivation method of a kind of roxburgh anoectochilus terminal bud seedling as claimed in claim 4, is characterized in that: described roxburgh anoectochilus terminal bud seedling is be highly the seedling of 5 ~ 7cm.
6. the cultivation method of a kind of roxburgh anoectochilus terminal bud seedling as claimed in claim 4, is characterized in that: described nanometer bamboo vinegar dilution is stoste dilute with water 200-400 times.
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CN104106357B (en) * 2014-06-25 2016-01-27 东源县瑞平生态农业发展有限公司 The breeding method of a kind of gold thread maple
CN104137727B (en) * 2014-07-28 2016-03-30 厦门加晟生物科技有限公司 A kind of implantation methods of roxburgh anoectochilus terminal bud
CN104277964A (en) * 2014-10-21 2015-01-14 皖西学院 Method for preparing anoectochilus formosanus vinegar through liquid fermentation
CN105052703A (en) * 2015-08-13 2015-11-18 江苏碧云天农林科技有限公司 Neofinetia falcata cultivating method
CN105123758B (en) * 2015-09-08 2018-07-06 福州麦克纳诺生物科技有限公司 A kind of nanometer of bamboo vinegar is as the application in plant growth regulator
CN108668836A (en) * 2018-04-28 2018-10-19 福建省大山金线莲科技开发有限公司 A kind of cultivation matrix and its cultural method of the nursery of roxburgh anoectochilus terminal bud high viability
CN109644851A (en) * 2019-01-29 2019-04-19 福建农林大学 A kind of method of factory culture roxburgh anoectochilus terminal bud

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CN101213942B (en) * 2008-01-10 2012-03-07 浙江省中药研究所有限公司 Medicinal anoectochilus Formosan tissue culture one-step seedling establishment fast propogation method
CN101233808B (en) * 2008-03-07 2010-12-08 浙江省中药研究所有限公司 Medicinal anoectochilus formosan stem-cutting root-retaining regeneration planting method
CN103340068B (en) * 2013-06-06 2015-05-13 福建省农业科学院农业生物资源研究所 Undergrowth cultivation medium and cultivation method for anoectochilus formosanus

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