CN109429966A - Simplified polygonatum kingianum seed seedling culture method with short period - Google Patents

Simplified polygonatum kingianum seed seedling culture method with short period Download PDF

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Publication number
CN109429966A
CN109429966A CN201811541331.0A CN201811541331A CN109429966A CN 109429966 A CN109429966 A CN 109429966A CN 201811541331 A CN201811541331 A CN 201811541331A CN 109429966 A CN109429966 A CN 109429966A
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China
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seed
polygonatum kingianurn
polygonatum
seedling
matrix
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Inventor
童清
杨利华
唐红燕
李倩
贾平
史富强
张朝玉
李宁华
戴林利
张建珠
赵俊波
罗罕艳
彭加萍
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PU'ER INSTITUTE OF FORESTRY SCIENCES
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention relates to the technical field of medicinal plant cultivation, in particular to a simplified polygonatum kingianum seed seedling raising method with a short period. The prior polygonatum kingianum planting technology adopts a series of complex treatment processes in order to break the dormancy stage of polygonatum kingianum seeds, and experiments prove that the process of removing fruit peels and refrigerating the seeds is omitted, the germination time of the polygonatum kingianum seeds is not influenced, and the germination rate of the seeds which are not treated is higher than that of the seeds which are removed the fruit peels and refrigerated; the invention adopts the coconut coir as the culture medium for the emergence of seedlings after the polygonatum kingianum sprouts, the emergence time is shortened, and the emergence rate is increased. The invention provides a simplified polygonatum kingianum seed seedling raising method, which achieves unexpected effects, shortens the seedling raising period and improves the germination rate and the emergence rate.

Description

A kind of simplification and period short polygonatum kingianurn seed seedling-raising method
Technical field
The present invention relates to technical field of medicinal plant cultivation, and in particular to a kind of simplification and period short polygonatum kingianurn seed is educated Seedling-growing method.
Background technique
Polygonatum kingianurn (Polygonatum kingianum Coll.et Hemsl) is Liliaceae Polygonatum herbaceous plant, root The nearly cylindrical or close beaded of shape stem, tubercle makees irregular water chestnut shape sometimes, plump.Berry is red, 1-1.5 centimetres of diameter, has 7- 12 seeds.The month at florescence 3-5, the fruiting period 9-10 month.It is recorded according to " Chinese Pharmacopoeia " version in 2015, Chinese medicine rhizoma polygonati is polygonatum kingianurn, Huang The dry rhizome of essence or polygonatum cyrtonema.Base kind one of of the polygonatum kingianurn as rhizoma polygonati, rhizome is more coarse, is rhizoma polygonati medicinal material In one of top-quality kind.Polygonatum kingianurn is distributed mainly on Vietnam, Burma in foreign countries, and China's main product is in Yunnan, Sichuan, expensive State, Guangxi etc. save, and wherein Yunnan is the genuine place of production of polygonatum kingianurn, and reserves are big, best in quality.
In recent years, as that studies polygonatum kingianurn deepens continuously, all kinds of values of polygonatum kingianurn are also constantly excavated, application Field is also constantly being expanded, especially medicine, health care, in terms of.Polygonatum kingianurn market demand is increasing, but existing Some market supplies can not fully meet market demand.Polygonatum kingianurn market is supplied mainly by wild resource, but wild Yunnan Rhizoma polygonati growth is very slow, under natural situation, sprouts from seed to the time that can excavate needs 6 years or so, and yield is extremely unstable It is fixed.Another supply source of polygonatum kingianurn is artificial growth, but artificial growth still belongs to the preliminary stage of fumbling at present, still lacks Weary scientific and efficient seedling-raising technique.
Existing polygonatum kingianurn planting technology is in order to break dormant period of polygonatum kingianurn seed, using the processing of a series of complex Process, the processing means reported include washing the extraseminal pericarp of removal by rubbing with the hands, mixing with wet sand mixing low temp stored for months, with sand Conjunction is polished etc. using machine.Above-mentioned treatment process is relatively complicated, and is easy to make polygonatum kingianurn seed during processing At damage, bud ratio is influenced.Further, many polygonatum kingianurn growers think that the extraseminal pericarp of polygonatum kingianurn sprouts seed at present Hair produces mechanical resistance, the sprouting of seed is hindered, so can all remove extraseminal pericarp before planting and be refrigerated.This Broken suspend mode processing is so that planting process is cumbersome, time-consuming a bit.
Summary of the invention
In view of the above shortcomings of the prior art, the purpose of the present invention is to provide a kind of simplification and period short polygonatum kingianurn kinds The process of removal pericarp and seed refrigeration, Yunnan is omitted in sub- method for culturing seedlings, polygonatum kingianurn seed seedling-raising method provided by the present invention The germinating time of rhizoma polygonati does not extend but germination percentage improves;The present invention is using coco bran as the cultivation base emerged after polygonatum kingianurn germination Matter, seedling-growing time shorten, and emergence rate increases.
Technical scheme is as follows:
A kind of polygonatum kingianurn seed seedling-raising method, comprising the following steps:
(1) seed is picked
Plucking time is September part, and seed source is 3~4 years raw Yunnan of growth and development stalwartness, full seed, no disease and pests harm Rhizoma polygonati plant, can standard of plucking: the extraseminal pericarp of polygonatum kingianurn becomes red by green, picking method: is adopted with tools such as secateurs Collect mature polygonatum kingianurn fruit, and deposits in ventilative container;
(2) sowing germination
The polygonatum kingianurn seed picked is not processed and can be sowed, sow ground selection: 15-26 DEG C of temperature range, Average annual rainfall 1000-1400mm, sunshine time 1990-2100h, test carry out under outdoor room temperature, make matrix with river sand, will Polygonatum kingianurn seed is spread out in sand bed, and the river sand of 1cm left and right thickness is covered on seed, keeps river sand wet;
(3) nursery is transplanted
Transplanting seedling medium used is one of coco bran, river sand, laterite or mixed-matrix, the composition of the mixed-matrix And weight ratio is river sand: coco bran=2:1 either laterite: coco bran=2:1.
Preferably, the average annual rainfall of above-mentioned polygonatum kingianurn seed seedling-raising method, selected sowing ground is 1340.9mm, Sunshine time is 2073.0h.
Preferably, above-mentioned polygonatum kingianurn seed seedling-raising method, transplanting matrix used in nursery is coco bran.
Further, above-mentioned polygonatum kingianurn seed seedling-raising method is added with biocontrol agent in coco bran matrix used.
Further, the microorganism of polygonatum kingianurn growth is beneficial in above-mentioned biocontrol agent.
The utility model has the advantages that
Polygonatum kingianurn seed seedling-raising method provided by the present invention overcomes existing polygonatum kingianurn planting process very complicated, educates The disadvantages of seedling period is long, output seedling is of poor quality, planting cost is low, can industrialized planting.
Nursery implantation methods exploratory experiment shows:
1, after planting developmental state
Under conditions of embodiment, polygonatum kingianurn the storage of seeds is sowed again after 2~3 months, and germinating time is about 90 days, is gone Except being sowed again after pericarp, germinating time is also about 90 days.Test prove, polygonatum kingianurn seed whether remove pericarp, whether Storing (storage time is within 3 months) influences less germinating time.Under normal circumstances, polygonatum kingianurn seed from be seeded into germination 3 months or so time is needed, germination process continues 1 month or so.
2, after planting germination percentage
After polygonatum kingianurn seed collection with no treatment, vernalization is directly disseminated in river sand, germination percentage highest is 79.33%;Germination test is carried out again by drying storage 2~3 months after the removal of polygonatum kingianurn seed pericarp, and germination percentage is minimum, only 34.00%.
3, emergence after transplanting
The polygonatum kingianurn seed to have germinateed is transplanted to and continues to cultivate in matrix, 3 months or so after transplanting start to emerge, The polygonatum kingianurn seed sprouting time is earliest in middle coco bran, and average seedling-growing time is 75d, and emergence is neat, and kind seedling leaf is generous, and color is deep It is green, robust growth;The latest, the seedling-growing time that is averaged is 83d to seedling-growing time, uneven emergence, but seedling early growth is more strong in laterite It is strong;River sand, river sand+coco bran, the polygonatum kingianurn seed seedling-growing time that is averaged is 78d in laterite+coco bran, but seedling is compared to its elsewhere Reason group leaf color is more shallow, and growing way is slightly weak.
4, emergence rate after transplanting
Polygonatum kingianurn seed emergence rate highest in coco bran is 98.67%, and emergence rate is minimum in laterite (control), is 87.33%.Although polygonatum kingianurn seeding ratio has reached 90.67% in river sand, seedling color is more shallow, and growing way is slightly weak.
Inventor obtains this best polygonatum kingianurn seed seedling-raising method by sowing and seedling growth test.From picking seed to out The time of seedling is 165 ± 30 days, greatly improves the seedling-growing time of existing polygonatum kingianurn implantation methods, and germination percentage and emergence Rate has extremely significant property to improve (P < 0.01) relative to existing routine techniques.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with implementation of the invention Example, technical scheme in the embodiment of the invention is clearly and completely described.Based on the embodiments of the present invention, this field Those of ordinary skill's every other embodiment obtained without making creative work, belongs to protection of the present invention Range.
Embodiment one: simplified polygonatum kingianurn seed seedling-raising method
(1) seed is picked
For the polygonatum kingianurn seed collection of the present embodiment from Puer City, acquisition time is September part.Select growth and development stalwartness, seed Full, no disease and pests harm 3~4 years raw polygonatum kingianurn plant of grain are as seed collecting maternal plant.Polygonatum kingianurn pericarp becomes red by green Picking.Mature polygonatum kingianurn fruit is acquired with tools such as secateurs, and is deposited in ventilative container.
(2) sowing germination
The present embodiment selection sowing and nursery be all Pu'er Institute of Forestry Sciences's nursery lot.
Puer City is located on the south the tropic of cancer, between 100 ° 19 '~101 ° 27 ' of east longitude, 22 ° 27 '~23 ° 06 ' of north latitude, belongs to Lower latitude plateau south subtropics monsoon climatic region has the characteristics that high temperature, rainy, and the winter, the summer, dry and wet was clearly demarcated without heat without severe cold, day According to abundance, abundant rainfall, 19.4 DEG C of average annual temperature, 25.6 DEG C of the highest temperature, 15.4 DEG C of the lowest temperature, sunshine time 2073.0h, Average annual rainfall 1340.9mm, frost-free period 315d.
The seed that step (1) is picked is without any processing, is directly used in sowing.When sowing, make matrix with river sand, by Yunnan Rhizoma polygonati seed is spread out in sand bed, and the river sand of 1cm left and right thickness is covered on seed, and routine observation germination keeps river sand wet Profit.Sowing ground is covered in outdoor, no greenhouse.
(3) nursery is transplanted
It is transplanted within after planting 90 ± 30 days, the polygonatum kingianurn seed to have germinateed in step (2) is transplanted to coco bran matrix Middle continuation nursery, nursery are covered in outdoor, no greenhouse.Other Cultivating techniques are identical as routine.
Embodiment two
The step of polygonatum kingianurn seed seedling-raising method and stated embodiment one of embodiment two, is identical, and difference is only to implement Biocontrol agent is added in coco bran matrix used in example two.The biocontrol agent title: I your benefit BMC biocontrol microorganisms;Manufacturer: lake My Er Li bioengineering Co., Ltd of north.Contain bacillus amyloliquefaciens and its generation for being grown in plant rhizosphere in the biocontrol agent Thank to product, bacillus amyloliquefaciens can produce exocellular polysaccharide, iturin isoreactivity substance, energy during growth and breeding Effective protection plant is from pathogen invasion.
Method of administration: before polygonatum kingianurn seed seedling-raising, my your benefit BMC biocontrol microorganisms is watered 800-1000 times and are uniformly sprayed on Coco bran after being sufficiently humidified so as to coco bran, then the polygonatum kingianurn seed of germination is transplanted in coco bran and carries out nursery.
More and more researches show that many beneficial microbes can protect plant from the infringement of pathogen, it is such micro- Biological such as bacillus amyloliquefaciens, bacillus subtilis, actinomyces, can be in prevention polygonatum kingianurn disease by biological control method It preserves the ecological environment while evil.
All microorganism formulations with the disoperation for protecting plant from pathogen are used equally in the present embodiment, And be not limited to the present embodiment stated one kind.
Embodiment three: best method for treating seeds is explored
The acquisition of 1 fruit is selected with nursery
The acquisition of 1.1 fruits
For polygonatum kingianurn seed collection from Puer City, acquisition time is September part.Select growth and development stalwartness, full seed, disease-free The raw polygonatum kingianurn plant in 3~4 years of insect pest is as seed collecting maternal plant.Polygonatum kingianurn pericarp, which becomes red by green, to be picked.Use secateurs The mature polygonatum kingianurn fruit of equal tools acquisition, and deposit in ventilative container.
The selection of 1.2 nursery
Nursery is selected in Pu'er Institute of Forestry Sciences's nursery lot.
Puer City is located on the south the tropic of cancer, between 100 ° 19 '~101 ° 27 ' of east longitude, 22 ° 27 '~23 ° 06 ' of north latitude, belongs to Lower latitude plateau south subtropics monsoon climatic region has the characteristics that high temperature, rainy, and the winter, the summer, dry and wet was clearly demarcated without heat without severe cold, day According to abundance, abundant rainfall, 19.4 DEG C of average annual temperature, 25.6 DEG C of the highest temperature, 15.4 DEG C of the lowest temperature, sunshine time 2073.0h, Average annual rainfall 1340.9mm, frost-free period 315d.
Method is probed into 2 tests
The processing of 2.1 seeds
The development of polygonatum kingianurn seed embryo in harvesting is not yet mature, has certain embryo morphological dormacy (after ripening);And The extraseminal pericarp of polygonatum kingianurn is hard, and general grower thinks that hard pericarp has certain mechanical resistance to the sprouting of seed Power, the method before being sowed usually using stack retting remove extraseminal red peel.This test is intended to by Yunnan Huang The extraseminal pericarp of essence carries out different disposal, observes treatment effect, explores and improves percentage of seedgermination, accelerates the best of nursery process Processing method.
This test grouping and processing method are as follows:
1st group: the extraseminal pericarp of polygonatum kingianurn is washed by rubbing with the hands completely, be placed in 4 DEG C of wet sands store it is spare;
2nd group: the extraseminal pericarp of polygonatum kingianurn being washed by rubbing with the hands completely, is stored in room temperature wet sand spare;
3rd group: by the fresh seeds adopted back without processing, retaining extraseminal pericarp, directly broadcast in outdoor sand bed Kind carries out germination test;
4th group (control conventional treatment method): the seed adopted back being placed on shady and cool indoor stack retting 5d~8d, then by fruit Skin is washed by rubbing with the hands completely, and seed, which is spread out, to dry in the shade, and is stored spare.
2.2 germination test
1st group and the 2nd group of polygonatum kingianurn seed carries out germination test, the 3rd group, the 4th group of Yunnan Huang after storage 2~3 months Smart seed carries out germination test immediately after treatment, and test carries out under outdoor room temperature.Make matrix with river sand, by polygonatum kingianurn seed It is spread out in sand bed, the river sand of 1cm left and right thickness is covered on seed.50 seeds of each processing group, 3 repetitions of every group of setting. It keeps river sand wet during test, institutes an inquiry germination after sowing 30d, hereafter statistics is primary every other week, judges to send out The standard of bud: radicle is prominent to break in the seed coat and length is more than seeds self length.
3 results and analysis
3.1 germination
Investigation discovery, polygonatum kingianurn the storage of seeds are sowed again after 2~3 months, and after planting 92d starts to germinate the 1st group of seed, the After planting 87d starts to germinate 2 groups of seeds, and germinating time continues 35d or so;Polygonatum kingianurn seed is directly sowed without storage, and the 3rd After planting 87d starts to germinate group seed, and after planting 90d starts to germinate the 4th group of seed, and germinating time continues 32d or so.
The experimental results showed that the germinating time of four processing groups is compared to each other, there is no significant difference, change and Yan Zhi, polygonatum kingianurn seed whether remove pericarp, whether store (storage time is within 3 months) germinating time is influenced it is little. Under normal circumstances, polygonatum kingianurn seed continues 1 month or so from germination 3 months or so time of needs, germination process is seeded into.
3.2 germination percentage
In the polygonatum kingianurn percentage of seedgermination of 4 kinds of different disposal groups of after planting 4 months statistics, 1 the results are shown in Table.
The germination percentage of 1 different disposal group polygonatum kingianurn seed of table
Note: I, II, III in upper table indicates that each processing group has 3 repeating groups;Average germination percentage refers to three repeating groups Germination quantity and sowing quantity ratio average.
Table 1 is not the results show that the 3rd group of polygonatum kingianurn percentage of seedgermination highest is made after polygonatum kingianurn seed collection in other words Any processing, directly disseminates vernalization in river sand, and germination percentage highest is 79.33%;It will dry in the air after the removal of polygonatum kingianurn seed pericarp Germination test is carried out again within dry-storage 2~3 months, germination percentage is minimum, and only 34.00%.
Variance analysis is carried out after the polygonatum kingianurn percentage of seedgermination of 4 processing groups is carried out arcsin transformation, the results are shown in Table 2.
The polygonatum kingianurn percentage of seedgermination variance analysis of 2 different disposal group of table
Table 2 the result shows that, 4 kinds of different disposal polygonatum kingianurn percentage of seedgermination F=43.227, P=0.000 < 0.01, difference Property is extremely significant.
For further analyze the difference between which group be it is significant, which be it is inapparent, inventor uses Multiple range test Further germination percentage is analyzed, the results are shown in Table 3.
The polygonatum kingianurn percentage of seedgermination Multiple range test of 3 different disposal group of table
Note: in upper table, lowercase reaction is 0.05 level of signifiance, and capitalization reaction is 0.01 extremely significant water It is flat.If the letter represented between two groups is not overlapped, different, then there is significant difference between two groups;The word represented between two groups Mother has repetition, then does not have significant difference between two groups.
The result of multiple comparisons shows the 3rd group of percentage of seedgermination otherness compared with the percentage of seedgermination of other 3 processing groups It is extremely significant, illustrate after polygonatum kingianurn seed collection with no treatment, directly to sow vernalization, energy in sand bed with fresh seeds Enough significantly improve percentage of seedgermination;And the processing group for removing pericarp either directly sows vernalization or cryopreservation 2~3 It is sowed again after month, there was no significant difference for germination percentage;Dry storage after seed is removed the peel, germination percentage are minimum.
The investigative test of embodiment two shows: the pericarp of polygonatum kingianurn seed is not the main original for influencing seed and sprouting Cause.Although the extraseminal pericarp of polygonatum kingianurn to the sprouting of seed there are certain mechanical resistance, polygonatum kingianurn seed it is permeable Property it is good, thus pericarp be not influence seed sprout the main reason for.The fresh seeds of harvesting are sowed directly in sand bed and are urged Bud can make the embryo of seed further reach maturity, and shorten the seed dormancy time, to improve the germination percentage of seed.
In conventional polygonatum kingianurn seed treatment, extraseminal pericarp will generally be removed, and in the mistake of pericarp removal Certain damage is inevitably caused in journey to seed, to influence the sprouting of seed.In addition, polygonatum kingianurn seed belongs to low recalcitrant Seed, therefore the dry storage or cryopreservation of either seed all will necessarily generate certain influence to the sprouting of seed.Cause This, the 3rd group is not processed the optimization process method that directly sowing is seed in polygonatum kingianurn nursery, and this method technical difficulty It is low, it is easy to operate, it is easy in seeling industry unit popularity.
Example IV: best seedling medium is explored
In common polygonatum kingianurn planting technology, after polygonatum kingianurn seed germinates in sand bed, be just transplanted in matrix into Row nursery.In order to filter out the matrix of optimum polygonatum kingianurn seed seedling-raising, embodiment three is provided with different substrates and carries out nursery examination It tests.
1 test method
Test grouping and matrix used are as shown in table 4:
Table 4 explores best seedling medium grouping and matrix used
Note: in upper table, coco bran is cocoanut husk fiber dust, is coconut by-product or waste after processing;River sand: coconut palm Chaff=2:1 indicates that the weight ratio of river sand and coco bran is 2:1;Laterite: coco bran=2:1 indicates that the weight ratio of laterite and coco bran is 2:1; Nursery is just used for after Combination matrix mixes well in proportion in table.
Same period germination and the identical chitting piece of state 50 are transplanted in every kind of matrix, and 3 repeating groups are set.It is removed between each group Matrix used is not identical, other rearing conditions are identical.Emergence is investigated after transplanting 30d, hereafter statistics is primary every other week, Emergence judgment criteria: bud is unearthed and rough leaf is unfolded.
2 results and analysis
2.1 emergence
Investigation discovery, the polygonatum kingianurn seed to have germinateed is transplanted to and continues to cultivate in matrix, is opened within 3 months or so after transplanting Begin to emerge, wherein polygonatum kingianurn seed sprouting is earliest in coco bran, and averagely seedling-growing time is 75d, and emergence rate is high, and emergence neatly, is planted Seedling leaf is generous, and color is dark green, robust growth;The latest, average seedling-growing time is 83d to seedling-growing time, and emergence rate is low, out in laterite Seedling is uneven, but seedling early growth is more healthy and strong;River sand, river sand+coco bran, polygonatum kingianurn seed is averaged seedling-growing time in laterite+coco bran For 78d, although polygonatum kingianurn seeding ratio has reached 90.67% in river sand, seedling is compared to other processing group blade face Color is more shallow, and growing way is slightly weak.
2.2 emergence rate
The polygonatum kingianurn seed to have germinateed is transplanted in 5 kinds of different matrix, emergence is investigated after 3 months, as a result It is as follows:
Polygonatum kingianurn seed sprouting situation in 5 different substrates of table
Note: I, II, III in upper table indicates that each processing group has 3 repeating groups;Average emergence rate refers to three repeating groups Dibbling number and number of emerging ratio average.
Table 5 is 98.67% the results show that polygonatum kingianurn seed emergence rate highest in coco bran, emergence rate in laterite (control) It is minimum, it is 87.33%.Make variance analysis after different substrates emergence rate data are carried out arcsin transformation, the results are shown in Table 6.
Polygonatum kingianurn seeding ratio variance analysis in 6 different substrates of table
Table 6 the result shows that, 5 kinds of different substrates polygonatum kingianurn seed seedling-raising emergence rate F=11.492, P=0.001 < 0.01, Otherness is extremely significant.Multiple range test is carried out to emergence rate, the results are shown in Table 7.
Polygonatum kingianurn seeding ratio Multiple range test in 7 different substrates of table
Note: in upper table, lowercase reaction is 0.05 level of signifiance, and capitalization reaction is 0.01 extremely significant water It is flat.If the letter represented between two groups is not overlapped, different, then there is significant difference between two groups;The word represented between two groups Mother has repetition, then does not have significant difference between two groups.
7 the result of multiple comparisons of table shows that polygonatum kingianurn seeding ratio is significantly higher than other processing groups when matrix is coco bran Emergence rate;River sand, river sand+coco bran, having a certain difference property between laterite+coco bran emergence rate, but otherness is not extremely Significantly;Polygonatum kingianurn seeding ratio is minimum in laterite.
The test result of embodiment three illustrates that polygonatum kingianurn nursery optimum substrate is coco bran.Coco bran has fabulous water conservation saturating Gas, meanwhile, coco bran is easier to obtain, and cheap, can be largely used among polygonatum kingianurn nursery stock production.
Place is not described in detail by the present invention, is the well-known technique of those skilled in the art of the present technique.Finally, it is stated that the above reality It applies example to be merely to illustrate explanation technical solution of the present invention rather than limit, modify to technical solution of the present invention or equivalent Replacement, without departing from the objective and range of technical solution of the present invention, is intended to be within the scope of the claims of the invention.

Claims (5)

1. a kind of polygonatum kingianurn seed seedling-raising method, it is characterised in that: the following steps are included:
(1) seed is picked
Plucking time is September part, and seed source is 3~4 years raw polygonatum kingianurns of growth and development stalwartness, full seed, no disease and pests harm Plant, can standard of plucking: the extraseminal pericarp of polygonatum kingianurn becomes red by green, picking method: with the acquisition of the tools such as secateurs at Ripe polygonatum kingianurn fruit, and deposit in ventilative container;
(2) sowing germination
The polygonatum kingianurn seed picked is not processed and can be sowed, and sows the selection on ground: 15-26 DEG C of temperature range, average annual Rainfall 1000-1400mm, sunshine time 1990-2100h, test carry out under outdoor room temperature, make matrix with river sand, by Yunnan Huang Smart seed is spread out in sand bed, and the river sand of 1cm left and right thickness is covered on seed, keeps river sand wet;
(3) nursery is transplanted
The polygonatum kingianurn seed to have germinateed in step (2) is transplanted in matrix and continues nursery, seedling medium used is coconut palm One of chaff, river sand, laterite or mixed-matrix, the composition and weight ratio of the mixed-matrix are river sands: coco bran=2:1 or It is laterite: coco bran=2:1.
2. polygonatum kingianurn seed seedling-raising method according to claim 1, it is characterised in that: the average annual drop on selected sowing ground Rainfall is 1340.9mm, sunshine time 2073.0h.
3. polygonatum kingianurn seed seedling-raising method according to claim 1, it is characterised in that: matrix used in transplanting nursery is coconut palm Chaff.
4. polygonatum kingianurn seed seedling-raising method according to claim 3, it is characterised in that: in coco bran matrix used in transplanting nursery It is sprayed with biocontrol agent.
5. polygonatum kingianurn seed seedling-raising method according to claim 4, it is characterised in that: containing beneficial in the biocontrol agent In the microorganism of polygonatum kingianurn growth.
CN201811541331.0A 2018-12-17 2018-12-17 Simplified polygonatum kingianum seed seedling culture method with short period Pending CN109429966A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109769613A (en) * 2019-03-27 2019-05-21 四川好土生物科技有限公司 A kind of polygonatum cyrtonema Industrialization of seeds and seedlings mating system
CN110521325A (en) * 2019-09-04 2019-12-03 昆明坤地农业开发有限公司 A kind of method of polygonatum kingianurn seed fast seedling growing
CN110537467A (en) * 2019-09-25 2019-12-06 贵州百灵企业集团制药股份有限公司 polygonatum kingianum seed greenhouse seedling growing method
CN112021116A (en) * 2020-09-04 2020-12-04 临沧道地中药材种植科技有限公司 Rapid breeding method of polygonatum kingianum
CN115250676A (en) * 2022-08-29 2022-11-01 重庆市合信农业科技有限公司 Seedling raising method for breaking dormancy stage of polygonatum sibiricum seeds

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106856726A (en) * 2015-12-10 2017-06-20 何开松 Sealwort is improved to emerge the propagation method of strong sprout rate and quality
CN108112434A (en) * 2017-12-12 2018-06-05 云南农业大学 A kind of pseudo-wild cultivating method of sealwort
CN108834801A (en) * 2018-06-12 2018-11-20 湖南衡岳中药饮片有限公司 A kind of method of rhizoma polygonati seed seedling-raising

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106856726A (en) * 2015-12-10 2017-06-20 何开松 Sealwort is improved to emerge the propagation method of strong sprout rate and quality
CN108112434A (en) * 2017-12-12 2018-06-05 云南农业大学 A kind of pseudo-wild cultivating method of sealwort
CN108834801A (en) * 2018-06-12 2018-11-20 湖南衡岳中药饮片有限公司 A kind of method of rhizoma polygonati seed seedling-raising

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
中国农业科学院果树研究所,柑桔研究所,郑州果树研究所编: "《中国果树栽培学》", 31 May 1987, 北京:农业出版社 *
中国医学科学院药用植物资源开发研究所云南分所编著: "《南药栽培技术 2》", 30 September 1984, 昆明:云南人民出版社 *
陈瑛主编: "《实用中药种子技术手册》", 30 June 1999 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109769613A (en) * 2019-03-27 2019-05-21 四川好土生物科技有限公司 A kind of polygonatum cyrtonema Industrialization of seeds and seedlings mating system
CN109769613B (en) * 2019-03-27 2021-07-20 四川好土生物科技有限公司 Polygonatum cyrtonema seedling industrialized breeding method
CN110521325A (en) * 2019-09-04 2019-12-03 昆明坤地农业开发有限公司 A kind of method of polygonatum kingianurn seed fast seedling growing
CN110537467A (en) * 2019-09-25 2019-12-06 贵州百灵企业集团制药股份有限公司 polygonatum kingianum seed greenhouse seedling growing method
CN112021116A (en) * 2020-09-04 2020-12-04 临沧道地中药材种植科技有限公司 Rapid breeding method of polygonatum kingianum
CN115250676A (en) * 2022-08-29 2022-11-01 重庆市合信农业科技有限公司 Seedling raising method for breaking dormancy stage of polygonatum sibiricum seeds

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