CN106613274A - Seedling raising method achieved by means of aconitum kusnezoffii Reichb. seeds - Google Patents
Seedling raising method achieved by means of aconitum kusnezoffii Reichb. seeds Download PDFInfo
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- 238000012258 culturing Methods 0.000 claims abstract description 16
- 230000008569 process Effects 0.000 claims abstract description 13
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- 238000005286 illumination Methods 0.000 claims abstract description 9
- 239000010455 vermiculite Substances 0.000 claims abstract description 9
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 9
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 9
- 239000004576 sand Substances 0.000 claims description 43
- 238000007605 air drying Methods 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 4
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- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 7
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a seedling raising method achieved by means of aconitum kusnezoffii Reichb. seeds. The method comprises the following steps of 1 seed pretreating, wherein stratification treatment is conducted on the aconitum kusnezoffii Reichb. seeds, the treatment temperature ranges from minus 5 DEG C to minus 1 DEG C, and the treatment time is 40-45 days; 2 seedling raising in containers, wherein a seedling raising medium is contained in the containers, the seedling raising medium is prepared from flower cultivation soil and vermiculite and thoroughly irrigated with water, the seeds obtained after treatment in the step 1 are sown in the containers, covered with soil and cultured under illumination, seedlings emerge after culturing is conducted for 3-5 days, and sufficient sunlight is guaranteed after the seedlings emerge. The technical method for conducting breeding and seedling raising by means of the aconitum kusnezoffii Reichb. seeds is provided in order to solve the problem that wild cultivation and domestication of characteristic Mongolian medicine plant aconitum kusnezoffii Reichb. are difficult, the germination rate is increased and the seedling uniformity is improved through a precise seed preteating technique, the survival rate of the seedlings is increased by means of reasonable management and protection in a seedling period, the cost is low, the propagation technique difficulties generated in the process that the aconitum kusnezoffii Reichb. is cultivated instead of growing in the wild are solved, and rapid propagation and large-scale production are achieved.
Description
Technical field
The invention belongs to RADIX ACONITI KUSNEZIFFII planting technology field, and in particular to a kind of RADIX ACONITI KUSNEZIFFII seed seedling-raising method.
Background technology
RADIX ACONITI KUSNEZIFFII (Aconitum kusnezoffii Reichb.) is widely used in anaesthetic clinic pharmacy, without radix aconiti agrestis
The Prescription of Mongolian Medicine of head about 1/3rd will be unable to use, but at present the medicinal source of RADIX ACONITI KUSNEZIFFII relies primarily on wild resource, difficult
To meet the market demand.It is that clinical pharmacy brings great risk and alkaloid is widely different in different sources RADIX ACONITI KUSNEZIFFII.
The at present rare a small amount of research of planting technology with regard to RADIX ACONITI KUSNEZIFFII (Aconitum kusnezoffii Reichb.) and
Science report, main method is that root tuber is transplanted and sowed.Root tuber breeding is applied in small range production, dragon plaintext (radix aconiti agrestis
Cultivation technique [J]. rural economy and technology, 2003,10:032), Chen Yubao (northern rhizome of Chinese monkshood cultivation management technology [J]. it is extraordinary economical
Animals and plants, 2014,17 (6):42-43.) etc. this method is reported, in First Year 9 to October wild RADIX ACONITI KUSNEZIFFII block has been cut out
Root, selection bud head is full, healthy no disease and pests harm root tuber is sowed in November then to December, or stored to Second Year 3 to April
Sowed.Although this method is easily survived, root tuber limited amount, breeding coefficient is low, and kind after repoductive time length
Easily degenerate, can not fundamentally solve the technical problem of RADIX ACONITI KUSNEZIFFII large area production.
Yang Guangmin et al. is with regard to the discussion of radix aconiti agrestis：Radix aconiti agrestis can break seed dormancy using autumn sowing or seedling transplanting technique
Processing method be in the test germination percentage of outdoor lamination 89 days be 80%.The technology has two：One, before the works
The textual criticism of text thinks that the base plant of radix aconiti agrestis is mainly the rhizome of Chinese monkshood (Aconitum carmichaeli Debx.) and the northern rhizome of Chinese monkshood
(Aconitum kusnezoffii Reichb.), in addition with other 14 kinds of aconitum plants the original of radix aconiti agrestis is equally accredited as
Plant, does not indicate it is any plant in text to the test that seed dormancy is released.According to Fan Yani (Northern Part of China parts
Aconitum plant Germplasm Resource Investigation and its introduce a fine variety and breed research [D]. Beijing:Beijing Forestry University, 2007.) with Zhou Lixia (crows
The resource investigation and introducing and planting research [D] of head category Activities of Some Plants. Beijing:Beijing Forestry University, research 2008), Aconitum
There is different degrees of dormancy in vegetable seeds, general to be released seed and be stopped by gibberellin seed soaking or cool stratification method
Sleep, but it is not of the same race different with the sensitivity of K cryogenic treatment to gibberellin, improve the most suitable gibberellin concentration of seed germination rate
It is also different with the optimization process time, wherein the dormancy degree of the northern rhizome of Chinese monkshood (Aconitum kusnezoffii Reichb.) seed is most
Deep, conventional treatment can not breaking dormancy.Two, this method for treating seeds can only can be grasped under the suitable environment of winter then
Make, process time is long, from environment and temporal restricted all than larger.Chen Yubao etc. chooses facing south for illumination abundance in Jilin
Bred to gentle slope, after seed is harvested in First Year late September to early October, after being soaked 5 hours with 500mg/L gibberellin
Cold stratification, sows first 1～2 week in Second Year the first tenday period of a month in May and is sowed after 25 DEG C of illumination boxs carry out vernalization process, and 8
Emerge within～12 days, emergence rate more than 94%.This method is very clear and definite, but for treatment temperature and SPT do not have
Clearly discuss, and gibberellin processes seed and increased operational sequence and gibberellin cost is higher, if grain weight is big, production cost with
Increase.
The content of the invention
The present invention is directed to problem above, it is determined that RADIX ACONITI KUSNEZIFFII (Aconitum kusnezoffii Reichb.) releases seed
The clear and definite temperature conditionss of dormancy and process time simultaneously carry out container nursery, and breeding coefficient is greatly improved, and is not in kind
The problem of degeneration, is the basic method of following RADIX ACONITI KUSNEZIFFII GAP.
The main contents of the present invention include Seeds preprocess technology, three aspects of Container nursery technique and seedling management,
The cultivation of RADIX ACONITI KUSNEZIFFII seedling is carried out by these steps, for the seedling that follow-up field-transplanting provides abundance.
The concrete technical scheme that the present invention is adopted is as follows：
A kind of RADIX ACONITI KUSNEZIFFII seed seedling-raising method, comprises the following steps：
(1) Seeds preprocess：RADIX ACONITI KUSNEZIFFII seed is carried out into Stratificated treatment, treatment temperature is -5～-1 DEG C, and process time is
40～45 days；
(2) container nursery：Load seedling medium in container, the seedling medium pours permeable by spending soil and vermiculite to constitute；
Seed after processing in step (1) is sown in container, earthing is cultivated under light illumination；Culture is emerged for 3～5 days；After emerging
Ensure sunny.
In step (1), because RADIX ACONITI KUSNEZIFFII seed has the Morphology And Physiology dormancy of deeper degree, unprocessed or processing mode
Irrational seed is not sprouted completely or germination rate is low, it is poor to sprout regularity, so as to affect emergence rate and Phase ofsprouting, institute
Suitably pre-processed with the seed demand before germination.Due to aconitum plant species it is various, each aconitum plant kind
The physiological property of son is all entirely different.The present invention is the physiological property according to RADIX ACONITI KUSNEZIFFII seed, research be improved emergence rate and
The method for culturing seedlings of the RADIX ACONITI KUSNEZIFFII seed of Phase ofsprouting.
At present the method for some existing Stratificated treatment seeds is typically placed in seed in 1～10 DEG C of temperature, but Jing
Overtesting is studied, and this Stratificated treatment mode is not effective to the dormancy for releasing RADIX ACONITI KUSNEZIFFII seed.And the invention
By lamination temperature setting at -5～-1 DEG C, effect is very notable.During Stratificated treatment of the present invention, in suitable temperature and humidity
Under so that RADIX ACONITI KUSNEZIFFII seed completes a series of physiology Aging storage, and the growth inhibitory substance content for enabling to seed declines,
And act on homogeneous so that the Phase ofsprouting of seed is high.RADIX ACONITI KUSNEZIFFII seed has Morphology And Physiology combinational dormancy, and Interior Seed
There is water-soluble and alcohol-soluble mortifier, this causes seed dormancy degree deeper, and wild RADIX ACONITI KUSNEZIFFII seed dormancy degree differs
Cause, conventional method is difficult to breaking dormancy.Under the conditions of suitable low temperature and moisture, RADIX ACONITI KUSNEZIFFII seed by complete a series of forms and
Physiology After-ripening, embryo grows to enough volumes, embryo form to be broken up completely, and mortifier hydrolysis release in seed, seed starts
Sprout.Through such process, RADIX ACONITI KUSNEZIFFII seed is not only solved because dormancy is difficult to the problem germinateed, while also solving radix aconiti agrestis
Head seed is because of the inconsistent caused germination of dormancy degree and irregular problem of emerging.By the solution of the two problems, make a living
Produce seminal propagation seedling stage in application to unify to manage and protect to provide convenience and premise.
Seed is selected：The seed that selection is harvested then, natural air drying, threshing cleaning.
In Stratificated treatment, for the selection of fine sand：The different-grain diameter grains of sand are different to seed Fertility effect, and the present invention is to fine sand
The requirement of particle diameter is less than or equal to 0.8mm.Fine sand humidity also has certain impact for Stratificated treatment seed, and the present invention is through sieve
It is 28～32% that choosing optimization obtains the humidity of fine sand, and through verification experimental verification, RADIX ACONITI KUSNEZIFFII seed can be carried out preferably under the humidity
Physiology after-ripening.The judgement holding of fine sand humidity is agglomerating, uniformly scatters after falling on the ground.The pH value of fine sand is 6.0-7.5, meets grass
The environmental requirement of aconitum seeds breaking dormancy state.
In Stratificated treatment, the mass ratio of the seed and fine sand is 1：3～4, it is preferred that the matter of the seed and fine sand
Amount ratio is 1：3.
In Stratificated treatment, spread the fine sand of 1.5～2.5cm thickness in container bottom first, be then placed in the seed mixed and
To 4～5cm of vessel port, in the fine sand for covering 3.5～4.5cm above, continue covering makes the domed projection of fine sand to fine sand, increases
Cladding thickness can reduce moisture loss, and in the container top for installing gas permeability covering (such as Nylon Bag, cloth) is added a cover again.
1～2 inspection is carried out during Stratificated treatment, after opening covering, if fine sand is dried, in surface watering can
Spray a small amount of water, it is ensured that fine sand humidity is basically unchanged；After checking out, covering is covered rapidly, put back to -5～-1 DEG C of insulating box
In.
After Stratificated treatment 40～45 days, the container that will be equipped with seed places 6～8h of defrosting in the cool, then removes covering
The fine sand that thing and surface cover, fine sand and seed are spread out, thin with a diameter of 1mm after air-drying 20～30 minutes in the cool
Sieve sifts out in seed, is sowed.
When stratification duration was more than 45 days, germination percentage will not be significantly improved, and more than 45 days, be found out
Seedling regularity is greatly lowered, and under conditions of highly dense, high yield, improves population growth regularity and have highly important meaning
Justice；If stratification duration is shorter than 40 days, the germination percentage of RADIX ACONITI KUSNEZIFFII seed is significantly reduced.Preferably, it is different to be optimal
Effect, process time be 6 weeks.
In step (2), container nursery is exactly the seedling raising mannerses that crop is cultivated with special container, and container fills nutrient and enriches
The matrix such as compost, make grow acquisition preferably nutrition and the environmental condition of seedling.
The selection of matrix is for emerging with large effect, it is contemplated that the stationarity of matrix, retentiveness, aeration, slow
Punching and the ability of offer nutrition, the present invention obtains being adapted to its solid-based according to the physiological growth characteristic screening and optimizing of RADIX ACONITI KUSNEZIFFII
Matter, the unit weight and porosity of the solid matrix, it is possible to increase the content of air and moisture.Preferably, the matrix is by Hua Tuhe
Vermiculite is according to mass ratio 1～2：1 composition, in order that emergence rate is higher, it is further preferred that described spend the native quality with vermiculite
Ratio is 1：1.The universal organic nutrient soil that soil is market sale is spent, main component is natural turf, perlite, sheep excrement, life
Organic fertilizer, coconut palm chaff etc., pH value is between 5.6～7.0, it is adaptable to various decorative flowers, foliage plant, various potted plant, vegetables
And seedling.
Cultivation temperature is arranged on 14～16 DEG C, it is preferred that cultivation temperature is 15 DEG C.
The present invention adopts container nursery so that RADIX ACONITI KUSNEZIFFII seedling percent is high, seedling-slowing stage is short, root of hair is fast, growth is vigorous.
The selection of container：Container for plant growth species is a lot, and shape, size, making material are also varied, can be according to Crop Species
The different of class, growing-seedling period, nursery stock specification etc. require to be selected.The present invention selects the plastic flowerpot of diameter 12cm.
Before sowing, soil and vermiculite will be spent to be well mixed and is fitted in container, it is desirable to dress soil height be away from above container 1.5～
At 2cm, each container pour it is permeable after dry in the air one day, moisten matrix in container but do not stick wet.Selecting the principle of moisture is,
Water content after drying in the air one day is the 60% of water content, after planting not make to be produced around seed water membrane as principle.
Preferably, 3 seeds are sown in each container, then 0.4～0.6cm of earthing (preferred 0.5cm), squirts surface.
Preferably, during culture, a water is poured within 2～3 days, watering will ensure to irrigate every time.
Preferably, intensity of illumination is more than 2LX, is easy to seedling normal growth.
Ensure after emerging it is sunny refer to emerge to rough leaf grow up to during ensure sunny, ground moistening；
Hereafter seedling stage manage and protect mainly appropriate shade, not only to ensure ground moistening, also to ensure with the humid air.Because Wild plant it is stingy
Time is waterside, sylvan life, border, and air humidity is big.
Have the advantages that in above-mentioned technical proposal：
(1) present invention adopts RADIX ACONITI KUSNEZIFFII seminal propagation, breeding coefficient to be significantly larger than root tuber propagation method.
(2) present invention adopts RADIX ACONITI KUSNEZIFFII seminal propagation, and plant variability is strong, variety deterioration will not occurs because of long-term plant
Phenomenon.
(3) present invention only need to be sowed after the completion of Seed Treatment, reduce labo r usage, reduced and produced into
This.
(4) wild cultivation of the present invention for characteristic anaesthetic plant RADIX ACONITI KUSNEZIFFII (Aconitum kusnezoffii Reichb.)
The difficult problem of training domestication, has invented a kind of technical method that breeding and seedling is carried out by RADIX ACONITI KUSNEZIFFII seed, by accurate seed
Preconditioning technique improves germination percentage and Phase ofsprouting, effect is significant, is rationally managed and protected by seedling stage and heightens survival rate of seedlings, and cost
It is low, RADIX ACONITI KUSNEZIFFII is solved by the reproduction technique difficulty in wild change cultivation, realize fast-propagation, large-scale production.
(5) according to the physiological growth characteristic of RADIX ACONITI KUSNEZIFFII seed, screening and optimizing of the present invention obtain temperature in Stratificated treatment and
Time, the dormancy to breaking seed has obvious facilitation, improves the germination percentage of RADIX ACONITI KUSNEZIFFII, and germination percentage reaches 92～94%
(3 days) and emergence rate are up to more than 96%, and (5 days, germination percentage referred to that the seed base-root through lamination is dashed forward and breaks in the seed coat and reach kind
The seed of sub- length accounts for the ratio for planting experimentally son；Emergence rate refers to the seed for showing money or valuables one carries unintentionally after planting, the plant number that cotyledon bassets
Account for the ratio of total sowing number), shorten seedling-growing time；Gibberellin, auxin etc. need not be used to promote the material of growth, it is ensured that
Phase ofsprouting, and reduce cost.
Description of the drawings
Fig. 1 is the RADIX ACONITI KUSNEZIFFII nursery photo in the embodiment of the present invention 1.
Specific embodiment
With reference to embodiment, the present invention is further described.
Embodiment 1
A kind of RADIX ACONITI KUSNEZIFFII seed seedling-raising method, comprises the following steps：
(1) Seeds preprocess technology
By the seed for harvesting then after natural air drying, threshing and cleaning, 30% fine sand is about according to 1 with humidity:3 ratio
Example is mixed thoroughly.Fine sand Particle size requirements：Sand of the aperture for 0.8mm dusting covers can be passed through；Fine sand humidity (30% or so) judges：Hold into
Group, uniformly scatters after falling on the ground.The pH value of fine sand is 6.0-7.5.The seed mixed is placed on into the breathables such as wooden case, flowerpot
In container, spread the damp sand of 1.5～2.5cm thickness in container bottom first, be then placed in the seed mixed and sand to vessel port 4～
At 5cm, in the damp sand for covering 3.5～4.5cm above, continuing covering makes the domed projection of damp sand, increases cladding thickness, reduces water
Dispersion is lost, and in the container top for installing gas permeability covering (such as Nylon Bag, cloth) is added a cover again.The seed for installing and container are put
Put 6 weeks in -5～-1 DEG C of insulating box, period can carry out 1～2 inspection, after opening covering, if fine sand is dried,
A small amount of water is sprayed with watering can in surface, it is ensured that fine sand humidity is substantially constant.After checking out, covering is covered rapidly, put back to insulating box
In.After 6 weeks, the container that seed is will be equipped with first is placed and thaw in the cool 6～8h, then removes what overcover and surface covered
Fine sand, damp sand and seed are spread out, after air-drying 20～30 minutes in the cool, are sifted out in seed with the dusting cover of a diameter of 1mm, enter
Row sowing.
(2) Container nursery technique
Medium of Container Seedling is to spend soil (containing agricultural gardening Development Co., Ltd purchased from Huaian grass)：Vermiculite is 1：1 soil
Matrix, container is the plastic flowerpot of diameter 12cm.Soil matrix is well mixed into loading flowerpot, it is desirable to which dress soil height is away from flowerpot
Top 1.5cm places, each flowerpot pour it is permeable after dry in the air 1 day, make basin soil moistening but glutinous wet.The seed handled well is sown into into flowerpot,
3 seeds are sown into per flowerpot, are uniformly put, then uniform earthing 0.5cm, with watering can surface earthing is squirted.Flowerpot is positioned over
On culturing rack with illumination, cultivation temperature is 15 DEG C, pours within 2～3 days a water, and watering will ensure to irrigate every time.Sowing 3～5
It is emerged, and emergence rate is up to 96%, it is desirable to nursery culture experiment room sunny (generally more than 2LX), if illumination is not enough,
Culturing rack light need to be opened after emerging 2 days carries out light filling.Emerge to rough leaf and grow up to sunny, the soil of period guarantee
Moistening；Hereafter seedling stage manage and protect mainly appropriate shade, not only to ensure ground moistening, also to ensure with the humid air.
The RADIX ACONITI KUSNEZIFFII seedling obtained using the method for culturing seedlings culture is as shown in Figure 1.
Comparative example 1
It is with the difference of embodiment 1：The seed for installing and container are placed on 6 weeks in 4 DEG C of insulating boxs, other are operated and work
Skill condition is same as Example 1.
Six weeks percentages of seedgermination of lamination are significantly reduced to 64% at 4 DEG C.
Comparative example 2
It is with the difference of embodiment 1：The seed for installing and container are placed on 2 weeks in -5～-1 DEG C of insulating box, other behaviour
Make and process conditions are same as Example 1.
2 weeks germination percentages only 36～40% are processed in -5～-1 DEG C of insulating box, the K cryogenic treatment time is inadequate, percentage of seedgermination
Significantly reduce.
Comparative example 3
It is with the difference of embodiment 1：Seedling medium be by spend soil and farmland soil according to mass ratio 1：1 mixing, other behaviour
Make and process conditions are same as Example 1.
Difference of the different seedling mediums on seedling-growing time is little, but seedling growing way (being primarily referred to as height of seedling) and survival rate of seedlings (seedling
Survival rate) between have larger difference, seedling survival rate=deposit number of live vaccine/sum × 100% of emerging.
Experimental example 1
Nursery is carried out to RADIX ACONITI KUSNEZIFFII using the method for culturing seedlings in embodiment 1, comparative example 1～3, germination percentage test is carried out.Inspection
Survey the results are shown in Table 1：
First gate time is sowing the 3rd day, and last gate time is sowing the 28th day.
Table 1
Table 2
| Object | Minute | Plant height (cm) | Launch the number of sheets |
| Embodiment 1 | Sow the 45th day | 5.9 | 1 |
| Comparative example 1 | Sow the 45th day | 5.5 | 1 |
| Comparative example 2 | Sow the 45th day | 6.1 | 1 |
| Comparative example 3 | Sow the 45th day | 4.5 | 1 |
Note：Plant height, 10 plants of averages that the expansion number of sheets is random searching.
Conclusion：By table 1 and Ke get, compared to comparative example 1～3, the method for culturing seedlings in embodiment 1 causes emerging for RADIX ACONITI KUSNEZIFFII
Rate is high, Phase ofsprouting is high, and height of seedling and seedling survival rate are significantly improved, wherein crucial factor is because embodiment 1 employs conjunction
The Stratificated treatment temperature and time of reason, and in seedling management, suitable seedling medium is employed, exactly grasp these passes
Key factor, just causes RADIX ACONITI KUSNEZIFFII nursery to have significant effect.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment
Limit, other any Spirit Essences without departing from the present invention and the change, modification, replacement made under principle, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of RADIX ACONITI KUSNEZIFFII seed seedling-raising method, is characterized in that, comprise the following steps：
（1）Seeds preprocess：RADIX ACONITI KUSNEZIFFII seed is carried out into Stratificated treatment, treatment temperature is -5 ~ -1 DEG C, and process time is 40 ~ 45
My god；
（2）Container nursery：Load seedling medium in container, the seedling medium pours permeable by spending soil and vermiculite to constitute；Will step
Suddenly（1）Seed after middle process is sown in container, and earthing is cultivated under light illumination；Culture is emerged for 3 ~ 5 days；Ensure after emerging
It is sunny.
2. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（1）In, in Stratificated treatment, the particle diameter of fine sand is less than
Equal to 0.8mm；The humidity of fine sand is 28 ~ 32%；The pH value of fine sand should be 6.0-7.5.
3. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（1）In, in Stratificated treatment, the seed and fine sand
Mass ratio be 1：3 ~ 4, it is preferred that the mass ratio of the seed and fine sand is 1：3.
4. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（1）In, in Stratificated treatment, first in container bottom
The fine sand of paving 1.5 ~ 2.5cm thickness, is then placed in the seed mixed and fine sand to 4 ~ 5cm of vessel port at, covering 3.5 above ~
The fine sand of 4.5cm, continuing covering makes the domed projection of fine sand, and increasing cladding thickness can reduce moisture loss, in the appearance for installing
Gas permeability covering is added a cover again in device top.
5. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（1）In, 1 ~ 2 inspection is carried out during Stratificated treatment,
After opening covering, if fine sand is dried, a small amount of water is sprayed with watering can on surface, it is ensured that fine sand humidity is constant；Check out
Afterwards, covering is covered rapidly, in putting back to -5 ~ -1 DEG C of insulating box.
6. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（1）In, Stratificated treatment will be equipped with planting after 40 ~ 45 days
The container of son is placed and thaw in the cool 6 ~ 8h, then removes the fine sand that overcover and surface cover, and fine sand and seed are spread out,
After air-drying 20 ~ 30 minutes in the cool, seed is isolated.
7. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（2）In, the seedling medium is by spending soil and vermiculite
According to mass ratio 1 ~ 2：1 composition, it is preferred that described to spend soil to be 1 with the mass ratio of vermiculite：1.
8. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（2）In, cultivation temperature is arranged on 14 ~ 16 DEG C, preferably
, cultivation temperature is 15 DEG C.
9. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（2）In, during culture, a water is poured within 2 ~ 3 days, every time
Watering will ensure to irrigate.
10. method for culturing seedlings as claimed in claim 1, is characterized in that：Step（2）In, intensity of illumination is more than 2LX.
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| CN111657084A (en) * | 2020-06-28 | 2020-09-15 | 国药种业有限公司 | Seedling raising method for aconitum sinomontanum seeds |
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| CN113207599A (en) * | 2021-05-06 | 2021-08-06 | 华邦建投集团股份有限公司 | Seedling raising method for leguminous plants in dry and hot valley region |
| CN113207599B (en) * | 2021-05-06 | 2023-10-27 | 华邦建投集团股份有限公司 | Seedling raising method for leguminous plants in dry-heat valley region |
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