CN102301901B - Sequoia sempervirens seed seedling culture method - Google Patents
Sequoia sempervirens seed seedling culture method Download PDFInfo
- Publication number
- CN102301901B CN102301901B CN2011102159095A CN201110215909A CN102301901B CN 102301901 B CN102301901 B CN 102301901B CN 2011102159095 A CN2011102159095 A CN 2011102159095A CN 201110215909 A CN201110215909 A CN 201110215909A CN 102301901 B CN102301901 B CN 102301901B
- Authority
- CN
- China
- Prior art keywords
- seedling
- seed
- bactericide
- wet
- sequoia sempervirens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention provides a Sequoia sempervirens seed seedling culture method, comprising the following steps: processing seeds, accelerating germination, preparing a medium, sowing, managing in a young seedling period, preparing nutritive soil, transplanting, managing after transplantation and the like. Through carrying out the step of accelerating germination before sowing, light, temperature, moisture and air required for the germination of Sequoia sempervirens seed are provided, and seed germination percentage and neatness are improved; soilless seedling culture is adopted to effectively solve the problem of damping-off disease in seedling stage; and the proper nutritive soil and nutrient solution are reasonably applied, and diseases are effectively prevented, thereby achieving higher planting percent, and guaranteeing seedling quality.
Description
Technical field
The present invention relates to a kind of plant seed seedling-cultivating method, particularly a kind of sequoia sempervirens seed seedling-raising method belongs to the plant propagation technical field.
Background technology
Sequoia sempervirens (
Sequoia sempervirens) have another name called world tree, seashore Chinese larch, evergreen Chinese larch, California Chinese larch, belong to Coniferales Taxodiaceae autogenus plant, be the rare few survivors seeds in the world.Having characteristics such as tall and big, evergreen, long-lived, that out-put is high, growth is rapid, is that famous speed is given birth to commerical tree species and huge sawmilling raw material of industry seeds.Its tree-like grace, tree performance is grand, and the four seasons are evergreen, are suitable for isolated planting or mass-planting in lakeside, the lawn, and grandeur is good afforestation seeds.Sequoia sempervirens in Shanghai, city cultivations such as Hangzhou, Nanjing, Foochow, Kunming, Kweiyang, it is good to introduce a fine variety the ground annidation at majority, has exploitation prospect.Yet the shortage of provenance is restricting applying of sequoia sempervirens always, and therefore numerous and confused expansion of domestic many mechanisms cultivated research to its breeding; But lay particular emphasis on vegetative propagation aspects such as cuttage, tissue culture, and seedling well developed root system, robust growth, the life-span of sequoia sempervirens are long, adaptive faculty is strong, plant type is graceful, have the advantage that the clone seedling hardly matches more; But the main dependence on import of its seed; Cost an arm and a leg, the seed sowing germination rate is low, and the conventional seedbed system method is prone to damping off in seedling stage (Rhizoctonia solani) simultaneously; Lethality reaches more than 10% ~ 50%, and planting percent is low.
Summary of the invention
For solving the provenance shortage, improve the sequoia sempervirens percentage of seedgermination, reduce the incidence of disease of damping off in seedling stage; Improve problems such as planting percent, the present invention provides a kind of raising germination rate, and the seedling phase does not have damping off and takes place; Planting percent is higher, and it is fast to emerge, and growing state is good; The sequoia sempervirens seed seedling-raising method that seedling is healthy and strong is realized through following technical proposal.
The present invention is to provide a kind of like this seedling-cultivating method, a kind of sequoia sempervirens seed seedling-raising method is characterized in that comprising the following steps:
1, a kind of sequoia sempervirens seed seedling-raising method is characterized in that comprising the following steps:
A. after the sequoia sempervirens seed being soaked 11～23 hours with clear water, carry out disinfection, clean, drain soaking seed by routine;
B. steps A gained seed being wrapped up with wet towel, is that 20～28 ℃, intensity of illumination are under 1500～3000Lx condition in temperature, and vernalization keeps towel moistening to seed sprouting during the vernalization;
C. the seed of step B is sowed on wet pearlite interstitial substance, perhaps sowed on the wet basis matter that perlite mixes with vermiculite, wherein the volume ratio of mixed-matrix is: Zhen Zhu Yan ︰ vermiculite=1～2 ︰ 1;
D. on the seed-bearing wet basis matter of sowing of step C, cover the wet basis matter identical with step C, the thickness that control covers wet basis matter is 0.3～0.8cm, is shading and is keeping under the matrix wet condition, cultivates seed to emerging;
E. by the seedling water spray of routine to step D; And bactericide of spray in per 3～4 days, after the week of emerging, it is drenched to spray mass concentration and be 0.05～0.1% nutrient solution to matrix; It is drenched to spray one time of nutrition liquid to matrix in every afterwards 5-7 days, grows 4～6 true leaves until seedling;
F. in sprigging to the conventional nutrition soil with step e; Water permeable; And growth is removed the condition of sheltering from heat or light after one week under the condition of sheltering from heat or light; Growing period sprays bactericide one time to seedling weekly, and watering weekly after become living and executing mass concentration is that 0.1～0.3% nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days.
The sterilization of said steps A is that to use mass concentration be that 0.1～0.3% potassium permanganate soaked seed 20～60 minutes; Perhaps using mass concentration is that 1～2% liquor natrii hypochloritis soaked seed 20～40 minutes.
The vernalization of said step B is to make chitting piece account for 5～30% of seed total amount to seed sprouting.
The thickness of identical wet basis matter is 0.3～0.8cm in the said step D covering.
The condition of sheltering from heat or light of said step D, F is: using the rate of shading is that 70～90% shading screen blocks.
Bactericide in the said step e is tpn, carbendazim, thiophanate methyl, agricultural streptomycin, each a kind of with wherein, and each bactericide is used alternatingly.
Nutrition soil in the said step F is to be mixed by laterite, perlite and the peat composed of rotten mosses volume ratio by 1 ~ 2 ︰, 1 ︰ 1.
Drench nutrition soil the previous day of sprigging to nutrition soil earlier in the said step F.
The bactericide of said step F is tpn, carbendazim, thiophanate methyl, mancozeb, each a kind of with wherein, and each bactericide is used alternatingly.
Said nutrient solution is commercial product.
The present invention has advantage and beneficial effect: vernalization in advance before the present invention sows, and provide and can satisfy the required light of sequoia sempervirens seed sprouting, temperature, wet, gas condition, improved percentage of seedgermination and regularity; Adopt soilless-cultivated seedling; Especially suitable sowing media; Efficiently solve damping off problem in seedling stage; See table 1 (different sowing medias are to the influence of damping off in seedling stage), cooperate effective prevention of rationally the using of appropriate nutrition soil and nutrient solution, disease again, can reach higher planting percent like this and guarantee the quality of seedling; For the breeding and seedling of sequoia sempervirens provides a kind of new, feasible solution, for standardization, the large-scale production that promotes the sequoia sempervirens seedling provides a new thinking; Use this method that a large amount of sequoia sempervirens high quality seedlings can be provided, and then produce huge ecological benefits and economic benefit.The seedling well developed root system of sequoia sempervirens, robust growth, the life-span is long, adaptive faculty is strong, plant type is graceful, has the advantage that the clone seedling hardly matches.
Embodiment
Below in conjunction with embodiment the present invention is done and to further describe.
Embodiment 1
A. after the sequoia sempervirens seed being soaked 23 hours with clear water, using mass concentration is that 0.1% potassium permanganate soaked seed 60 minutes, cleans, drain well;
B. the seed after steps A being drained wraps up with wet towel, is that 28 ℃, intensity of illumination are vernalization under the 2000Lx condition in temperature, spray water to towel every day simultaneously, and to keep towel moistening, be 5% until percentage of seedgermination;
C. the seed of step B is evenly sowed on wet pearlite interstitial substance;
D. on the seed-bearing wet basis matter of sowing of step C; Cover wet pearlite interstitial substance, the thickness that control covers wet basis matter is 0.3cm, is placed on the seedbed in the greenhouse; And above the seedbed, establishing the rate of shading and be 70% sunshade net shades; Pass through matrix with the clear water spray, and it is moistening to remain matrix, cultivates seed to emerging;
E. by the seedling water spray of routine to step D, by bactericide of routine spray in per 3 days, and bactericide is tpn, carbendazim, thiophanate methyl, agricultural streptomycin; Spray a kind of each time; Alternately spray, after the week of emerging, it is drenched to spray mass concentration and be 0.1% commercial nutrient solution to matrix; Per afterwards nutrient solution to matrix that sprayed an equal in quality concentration in 7 days drenches, and grows 4～6 true leaves until seedling;
F. with the: Hong Tu ︰ Zhen Zhu Yan ︰ peat composed of rotten mosses=1.5 ︰, 1 ︰, 1 volume ratio in sprigging to the following wet nutrition soil of step e; Water permeablely, and to use the rate of shading be that 80% sunshade net shades, one week the back remove the sunshade net; Growing period sprays bactericide by routine one time to seedling weekly; Bactericide is tpn, carbendazim, thiophanate methyl, mancozeb, sprays a kind ofly at every turn, and various bactericide alternately spray; The back of become living waters weekly by routine that to execute mass concentration be that 0.3% commercial nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days.
Embodiment 2
A. after the sequoia sempervirens seed being soaked 11 hours with clear water, using mass concentration is that 2% liquor natrii hypochloritis soaked seed 20 minutes, cleans, drain well;
B. the seed after steps A being drained wraps up with wet towel, is that 20 ℃, intensity of illumination are vernalization under the 3000Lx condition in temperature, spray water to towel every day simultaneously, and is 10% to keep towel moistening until percentage of seedgermination;
C. the seed of step B is evenly sowed on the wet basis matter that perlite mixes with vermiculite, wherein the volume ratio of mixed-matrix is: Zhen Zhu Yan ︰ vermiculite=2 ︰ 1;
D. on the seed-bearing wet basis matter of sowing of step C; Cover wet pearlite interstitial substance, the thickness that control covers wet basis matter is 0.8cm, is placed on the seedbed in the greenhouse; And above the seedbed, establishing the rate of shading and be 90% sunshade net shades; Pass through matrix with the clear water spray, and it is moistening to remain matrix, cultivates seed to emerging;
E. by the seedling water spray of routine to step D, by bactericide of routine spray in per 4 days, and bactericide is tpn, carbendazim, thiophanate methyl or agricultural streptomycin; Spray a kind of each time; Alternately spray, after the week of emerging, it is drenched to spray mass concentration and be 0.05% commercial nutrient solution to matrix; Per afterwards nutrient solution to matrix that sprayed an equal in quality concentration in 5 days drenches, and grows 4～6 true leaves until seedling;
F. in sprigging to the following wet nutrition soil with step e: give birth to the Hong Tu ︰ Zhen Zhu Yan ︰ peat composed of rotten mosses=2 ︰, 1 ︰, 1 volume ratio; Water permeablely, and to use the rate of shading be that 70% sunshade net shades, one week the back remove the sunshade net; Growing period sprays bactericide by routine one time to seedling weekly; Bactericide is tpn, carbendazim, thiophanate methyl and mancozeb, sprays a kind ofly at every turn, is used alternatingly and sprays; The back of become living waters weekly by routine that to execute mass concentration be that 0.1% commercial nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days.
Embodiment 3
A. after the sequoia sempervirens seed being soaked 18 hours with clear water, using mass concentration is that 1% liquor natrii hypochloritis soaked seed 40 minutes, cleans, drain well;
B. the seed after steps A being drained wraps up with wet towel, is that 28 ℃, intensity of illumination are vernalization under the 1500Lx condition in temperature, spray water to towel every day simultaneously, and is 30% to keep towel moistening until percentage of seedgermination;
C. the seed of step B is evenly sowed on the wet basis matter that perlite mixes with vermiculite, wherein the volume ratio of mixed-matrix is: Zhen Zhu Yan ︰ vermiculite=1 ︰ 1;
D. on the seed-bearing wet basis matter of sowing of step C; Cover wet pearlite interstitial substance, the thickness that control covers wet basis matter is 0.5cm, is placed on the seedbed in the greenhouse; And above the seedbed, establishing the rate of shading and be 80% sunshade net shades; Pass through matrix with the clear water spray, and it is moistening to remain matrix, cultivates seed to emerging;
E. by the seedling water spray of routine to step D, by bactericide of routine spray in per 4 days, and bactericide is tpn, carbendazim, thiophanate methyl or agricultural streptomycin; Spray a kind of each time; Alternately spray, after the week of emerging, it is drenched to spray mass concentration and be 0.08% commercial nutrient solution to matrix; Per afterwards nutrient solution to matrix that sprayed an equal in quality concentration in 6 days drenches, and grows 4～6 true leaves until seedling;
F. in sprigging to the following wet nutrition soil with step e: give birth to the Hong Tu ︰ Zhen Zhu Yan ︰ peat composed of rotten mosses=1.5 ︰, 1 ︰, 1 volume ratio; Water permeablely, and to use the rate of shading be that 80% sunshade net shades, one week the back remove the sunshade net; Growing period sprays bactericide by routine one time to seedling weekly; Bactericide is tpn, carbendazim, thiophanate methyl and mancozeb, sprays a kind ofly at every turn, is used alternatingly and sprays; The back of become living waters weekly by routine that to execute mass concentration be that 0.2% commercial nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days.
Embodiment 4
A. after the sequoia sempervirens seed being soaked 15 hours with clear water, using mass concentration is that 0.3% potassium permanganate soaked seed 20 minutes, cleans, drain well;
B. the seed after steps A being drained wraps up with wet towel, is that 22 ℃, intensity of illumination are that vernalization is sprayed water to towel to seed sprouting simultaneously every day under the 2500Lx condition in temperature, to keep towel moistening, reaches 22% until percentage of seedgermination;
C. the seed of step B is evenly sowed on wet pearlite interstitial substance;
D. on the seed-bearing wet basis matter of sowing of step C; Cover wet pearlite interstitial substance, the thickness that control covers wet basis matter is 0.6cm, is placed on the seedbed in the greenhouse; And above the seedbed, establishing the rate of shading and be 85% sunshade net shades; Pass through matrix with the clear water spray, and it is moistening to remain matrix, cultivates seed to emerging;
E. by the seedling water spray of routine to step D, by bactericide of routine spray in per 4 days, and bactericide is tpn, carbendazim, thiophanate methyl or agricultural streptomycin; Spray a kind of each time; Alternately spray, after the week of emerging, it is drenched to spray mass concentration and be 0.08% commercial nutrient solution to matrix; Per afterwards nutrient solution to matrix that sprayed an equal in quality concentration in 6 days drenches, and grows 4～6 true leaves until seedling;
F. in sprigging to the following wet nutrition soil with step e: give birth to the Hong Tu ︰ Zhen Zhu Yan ︰ peat composed of rotten mosses=1 ︰, 1 ︰, 1 volume ratio; Water permeablely, and to use the rate of shading be that 85% sunshade net shades, one week the back remove the sunshade net; Growing period sprays bactericide by routine one time to seedling weekly; Bactericide is tpn, carbendazim, thiophanate methyl and mancozeb, sprays a kind ofly at every turn, is used alternatingly and sprays; The back of become living waters weekly by routine that to execute mass concentration be that 0.2% commercial nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days.
The different sowing medias of table 1 are to the influence of damping off in seedling stage
Claims (7)
1. a sequoia sempervirens seed seedling-raising method is characterized in that comprising the following steps:
A. after the sequoia sempervirens seed being soaked 11～23 hours with clear water, carry out disinfection, clean, drain soaking seed by routine;
B. steps A gained seed being wrapped up with wet towel, is that 20～28 ℃, intensity of illumination are under 1500～3000Lx condition in temperature, and vernalization keeps towel moistening to seed sprouting during the vernalization;
C. the seed of step B is sowed on wet pearlite interstitial substance, perhaps sowed on the wet basis matter that perlite mixes with vermiculite, wherein the volume ratio of mixed-matrix is: Zhen Zhu Yan ︰ vermiculite=1～2 ︰ 1;
D. on the seed-bearing wet basis matter of sowing of step C, cover the wet basis matter identical with step C, the thickness that control covers wet basis matter is 0.3～0.8cm, is shading and is keeping under the matrix wet condition, cultivates seed to emerging;
E. by the seedling water spray of routine to step D; And bactericide of spray in per 3～4 days, after the week of emerging, it is drenched to spray mass concentration and be 0.05～0.1% nutrient solution to matrix; Every afterwards nutrient solution to matrix that sprayed an equal in quality concentration in 5-7 days drenches, and grows 4～6 true leaves until seedling;
F. in sprigging to the nutrition soil with step e; Water permeable; And growth is removed the condition of sheltering from heat or light after one week under the condition of sheltering from heat or light; Growing period sprays bactericide one time to seedling weekly, and watering weekly after become living and executing mass concentration is that 0.1～0.3% nutrient solution to nutrition soil is drenched, and growing promptly obtains the seedling of sequoia sempervirens after 80～100 days; Wherein nutrition soil is to be mixed by laterite, perlite and the peat composed of rotten mosses volume ratio by 1～2 ︰, 1 ︰ 1.
2. seedling-cultivating method according to claim 1 is characterized in that: the sterilization of said steps A is that to use mass concentration be that 0.1～0.3% potassium permanganate soaked seed 20～60 minutes; Perhaps using mass concentration is that 1～2% liquor natrii hypochloritis soaked seed 20～40 minutes.
3. seedling-cultivating method according to claim 1, the vernalization that it is characterized in that said step B are to make chitting piece account for 5～30% of seed total amount to seed sprouting.
4. seedling-cultivating method according to claim 1 is characterized in that: the condition of sheltering from heat or light of said step D, F is: using the rate of shading is that 70～90% shading screen blocks.
5. seedling-cultivating method according to claim 1 is characterized in that: the bactericide in the said step e is tpn, carbendazim, thiophanate methyl, agricultural streptomycin, each a kind of with wherein, and each bactericide is used alternatingly.
6. seedling-cultivating method according to claim 1 is characterized in that: drench nutrition soil the previous day of sprigging to nutrition soil earlier in the said step F.
7. seedling-cultivating method according to claim 1 is characterized in that: the bactericide of said step F is tpn, carbendazim, thiophanate methyl, mancozeb, each a kind of with wherein, and each bactericide is used alternatingly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102159095A CN102301901B (en) | 2011-07-29 | 2011-07-29 | Sequoia sempervirens seed seedling culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102159095A CN102301901B (en) | 2011-07-29 | 2011-07-29 | Sequoia sempervirens seed seedling culture method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102301901A CN102301901A (en) | 2012-01-04 |
| CN102301901B true CN102301901B (en) | 2012-11-14 |
Family
ID=45375906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102159095A Expired - Fee Related CN102301901B (en) | 2011-07-29 | 2011-07-29 | Sequoia sempervirens seed seedling culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102301901B (en) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385091B (en) * | 2013-06-28 | 2015-04-22 | 句容市茅山聚福园林有限公司 | Planting method for North America sweetgum |
| CN103798036B (en) * | 2014-02-08 | 2015-08-19 | 朱兰英 | The cultivation method of a kind of strawberry |
| CN103798143A (en) * | 2014-02-25 | 2014-05-21 | 云南省农业科学院花卉研究所 | Out-bottle cutting and rooting method for sequoia sempervirens tissue culture seedling |
| CN103931637B (en) * | 2014-04-25 | 2015-09-09 | 福建农林大学 | A kind of protectant improving peanut seed emergence rate |
| CN105354624B (en) * | 2015-10-08 | 2019-08-30 | 江苏绿田环保科技有限公司 | A method of the efficiency of prediction phytoremediation organic polluted soil |
| CN105453982A (en) * | 2015-12-16 | 2016-04-06 | 青岛百瑞吉生物工程有限公司 | Sowing propagation method for dawn redwoods |
| CN105613238B (en) * | 2015-12-29 | 2018-09-21 | 美尚生态景观股份有限公司 | A kind of hardening off method of sequoia sempervirens |
| CN105766522B (en) * | 2016-03-22 | 2018-12-21 | 广西生态工程职业技术学院 | A kind of processing method preventing and treating China Fir Seedling samping off |
| CN106508575A (en) * | 2016-11-10 | 2017-03-22 | 唐翔 | Keteleeria pubescens seedling raising method |
| CN106613652A (en) * | 2016-12-12 | 2017-05-10 | 广东林科种苗有限公司 | Cedar sterile sowing seedling growing method |
| CN106717292A (en) * | 2016-12-16 | 2017-05-31 | 常州市环境监测中心 | A kind of chamber tested for germination and root elongation inhibition |
| CN106665207B (en) * | 2016-12-22 | 2019-12-27 | 宣威市龙津生物科技有限责任公司 | Method for preventing occurrence of cataplexy of fleabane flower in floating seedling culture seedling stage |
| CN106900470A (en) * | 2017-04-25 | 2017-06-30 | 安庆师范大学 | Larix chinensis nursery and method of forestation |
| CN108940565A (en) * | 2018-06-24 | 2018-12-07 | 合肥扬扬农业科技有限公司 | A kind of seed production conveying vibrating screen impurity-removing method |
| CN109479568B (en) * | 2018-11-27 | 2021-01-15 | 湖北民族学院 | Planting tray and method for germination and seedling strengthening of metasequoia seeds |
| CN114711127B (en) * | 2022-03-21 | 2024-04-09 | 中国林业科学研究院高原林业研究所 | Seedling raising method for pinus massoniana |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451267A (en) * | 2003-05-17 | 2003-10-29 | 重庆远大生态农业发展有限公司 | Quick Taxus mandia propagating method |
-
2011
- 2011-07-29 CN CN2011102159095A patent/CN102301901B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451267A (en) * | 2003-05-17 | 2003-10-29 | 重庆远大生态农业发展有限公司 | Quick Taxus mandia propagating method |
Non-Patent Citations (2)
| Title |
|---|
| 李福秀 等.北美红杉不同个体间插穗生根能力研究.《林业调查规划》.2006,第31卷(第3期), * |
| 邵金平 等.北美红杉种子育苗试验.《云南林业科技》.2002,(第4期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102301901A (en) | 2012-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102301901B (en) | Sequoia sempervirens seed seedling culture method | |
| CN102119594B (en) | Method for promoting rhododendron parvifolium seed germination | |
| CN103081608B (en) | Germination processing method for horseradish seeds | |
| CN102599026B (en) | Tilia miqueliana seedling raising method | |
| CN103828717A (en) | Rapid propagating and seedling culturing method by virtue of Bletilla tubers | |
| CN103704130B (en) | A kind of method of Chunlan and the nursery of hybrid cymbidium crossbreed | |
| CN105325272A (en) | Lagerstroemia indica cutting breeding method | |
| CN103385083A (en) | Method for cultivating and strengthening eggplant seedlings in spring greenhouse | |
| CN103283599B (en) | Method for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province | |
| CN102090329B (en) | Method for transplanting Damascus rose tissue culture seedling | |
| CN103749137A (en) | Cutting seedling method for malus toringoides | |
| CN107980635A (en) | A kind of Tissue-cultured apple seedling two step method for transplanting of high-survival rate | |
| CN101715711A (en) | Method for growing pollution-free water vegetable | |
| CN103858637A (en) | Method of recycling and cultivating pachira macrocarpa | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN103875330A (en) | Method for rice cold-proof rice seedling raising | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN104770173B (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN102523875B (en) | Cuttage propagation method for Rhododendron ovatum (Lindl.) Planch. ex Maxim. | |
| CN106613079B (en) | production method of pinellia ternata seed stems | |
| CN104542284A (en) | Tissue culture rapid propagation method for rhododendron irroratum | |
| CN102934582A (en) | Pest and disease prevention and control management in germination period of hot peppers | |
| CN103098632A (en) | Pod pepper planting method | |
| CN104885932A (en) | Tissue culture and rapid propagation method for rhododendron moulmainense | |
| CN105494097A (en) | In-vitro rapid propagation technology of viburnum sargentii koehne |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120104 Assignee: Yunnan Jichuang Horticultural Technology Co., Ltd. Assignor: Flower Research Institute(FRI) of Yunnan Contract record no.: 2015530000049 Denomination of invention: Sequoia sempervirens seed seedling culture method Granted publication date: 20121114 License type: Exclusive License Record date: 20150519 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20200729 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |