CN115250814B - Method suitable for germination accelerating and seedling raising of rhododendron in Henan province - Google Patents
Method suitable for germination accelerating and seedling raising of rhododendron in Henan province Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Abstract
The invention discloses a germination accelerating and seedling raising method suitable for rhododendron in Henan, which adopts a PEG-6000 solution with the content of 2.5 percent to promote the germination of seeds in advance, improves the germination rate of the seeds by more than 80 percent and is not susceptible to diseases. The seedling raising adopts a shallow tray matrix layering method, namely matrix layering only adopts two types of matrixes, a layer of pinhole preservative film or plastic film (or professional water permeable film) is paved between the two matrixes, so that nutrient and moisture loss in the second matrix can be effectively prevented, and meanwhile, when the upper matrix is slightly drought, the moisture in the lower matrix plays a role of reverse osmosis through holes in the film, so that soil moisture is increased and soil moisture is preserved for the upper matrix, the irrigation times are reduced, and the maintenance cost is reduced. The seedling raising method is easy to operate, the seedling raising trays are various in variety and flexible in size, wastes such as snack boxes can be selected for recycling, the sprinkling frequency is reduced, water is saved, the maintenance cost is low, in addition, the seedling raising is not limited by time, and the emergence rate can reach more than 90%.
Description
Technical Field
The invention belongs to the technical field of plant seedling raising, and particularly relates to a germination accelerating seedling raising method suitable for rhododendron henryi.
Background
The rhododendron is a special species newly found in the mountain land of the Funiu in Henan province, and belongs to one of the rhododendrons in the mountain; the flower is evergreen in the whole year, the branches and trunk are strong, the flowers are big and beautiful, and the flower is a precious ornamental flower and tree. Due to the change of climate environment, the distribution elevation of the rhododendron in Henan is reduced from 1500 meters to more than 1800 meters, the suitable growth range is gradually reduced, and the highest elevation of Henan is only 2413.8 meters; in addition, the rhododendron seedling has severe requirements on living environment, the natural updating of the seedling under the wild forest of the rhododendron is rare, the whole number is gradually reduced, and the living is seriously threatened. Because the original environment condition of the wild Henan azalea is severe, the wild Henan azalea needs to be protected on the ground. The survival rate of cutting seedling is low and cutting collection is difficult; and the seeds in the capsules of the rhododendron in Henan are small and more, so that the rhododendron in Henan is suitable for seed propagation. The rhododendron Henryi seed has slow germination and low germination rate. By adopting four storage modes of low-temperature wet storage, low-temperature dry storage and normal-temperature dry storage, the 4 ℃ wet storage is found to be favorable for germination of rhododendron heyneanum seeds. However, excessive water in the germination process causes insufficient oxygen, which is unfavorable for germination, and even easily infects diseases to cause seed rot, so that the water content problem in the seed germination process is to be solved.
The traditional seedling bed seedling culture and plug seedling culture tests are adopted to find that: the traditional seedbed is adopted for seedling raising, the emergence rate of the seeds is 36-67% after 40 days, the germination rate of the seeds in 4 ℃ wet storage is 67% at most, and the dry storage at room temperature is as low as 36%; the germination rate of the plug seedling seeds is 51% -73%; the seedling rate after 80 days is lower. As the rhododendron seedlings are water-loving but not water-wetting, the soil is loose and breathable, and has good water-holding and water-draining properties. The seedling bed has poor air permeability and poor drainage, so that a large number of seedlings die in the later period, and the survival rate of the seedlings after 80 days of sowing is only about 12%; plug seedling, the water retention is poor, need continuous water spray maintenance, cause the waste, once cut off water and cause seedling dead easily, the survival rate of seedling is about 20% after 80 days of seeding. The rhododendron is cool, the traditional seedling culture is sprouted in 3 months and 4 months, and the survival rate of the seedlings is greatly reduced after 7 months and 8 months of high temperature. Therefore, the traditional seedling raising method is not suitable for the rhododendron henryi, and the seedling raising of the rhododendron henryi seeds is improved.
Disclosure of Invention
The invention aims to provide a method suitable for germination accelerating and seedling raising of rhododendron henryi, which can effectively improve the germination rate of rhododendron henryi seeds and the survival rate of seedlings.
In order to achieve the above object, the technical scheme of the present invention is summarized as follows:
a method suitable for germination accelerating and seedling raising of rhododendron in Henan, comprising the following steps:
(1) Seed treatment: drying collected wild Henan azalea capsules in the shade at a drying place, collecting seeds with full seeds and removing impurities when the capsules are naturally cracked, wrapping clean seeds in layers by wet towels, and storing the seeds in a refrigerator at a low temperature of 4 ℃ for standby, wherein the towels are kept moist all the time;
(2) Accelerating germination of seeds: uniformly spreading the rhododendron Henryi seeds treated in the step (1) in a culture dish or shallow dish with three layers of filter paper, carrying out water treatment by using a PEG-6000 solution with the content of 2.5%, setting the water potential of the solution at about-0.06 MPa, and carrying out germination acceleration by using a constant-temperature artificial climate incubator with the temperature of 20 ℃ or a variable-temperature artificial climate incubator with the temperature of 20-25 ℃, keeping the humidity at 70-80%, and replacing the filter paper every 3-4 days until radicle is broken;
(3) Shallow tray substrate layered seedling raising: selecting a shallow tray with the depth of 2-4cm, preparing two matrixes, namely a matrix I and a matrix II, uniformly spreading the matrix I on the bottom of the shallow tray, spraying purified water with the pH value of 6.0-7.0 (ferrous sulfate solution with the pH value of 1:1000 is used for adjusting the pH value), spreading a preservative film or a plastic film on the matrix I, or using a professional permeable film with the thickness of 0.1-0.2mm, wrapping the whole bottom and the peripheral tray walls of the shallow tray by the preservative film or the plastic film, and uniformly pricking small holes on the preservative film or the plastic film by using needles; uniformly spreading a layer of matrix II on the preservative film, spraying purified water, uniformly scattering the germinated seeds on the matrix II, covering a layer of thin and screened matrix II, sealing the shallow tray opening by the preservative film, and culturing in a constant-temperature artificial climate incubator at 20 ℃;
(4) Emergence and maintenance: pouring purified water once in 4-5 days, breaking the cotyledons for about 10 days to obtain the first true leaves, growing the first true leaves for 10-15 days, and when 2-4 true leaves grow in 8 months, half opening the seal in the early stage, wherein the ventilation seedling hardening time gradually increases from 1 hour per day to complete opening of the seal; and taking the shallow tray seedlings with the uncovered seals out of the artificial climate incubator for hardening off after 1 month, gradually increasing from 1 hour per day to completely adapt to the external environment, and transplanting the rhododendron seedlings for the first time when the average gas temperature of the moving average is reduced to 16-26 ℃.
Preferably, both substrates are sterilized at 120 ℃ for 20 minutes.
Preferably, the first matrix is mixed by the weight ratio of the loose soil to the turfy soil of 3:1, and the second matrix is mixed by the weight ratio of the turfy soil, the vermiculite, the loose soil and the perlite of 2:2:1:1.
Preferably, the thickness of the substrate is 1/4 of the depth of the tray, and the thickness of the substrate is 1/2 of the depth of the tray.
Preferably, the number of small holes is about 0.5 to 1/cm 2 。
Preferably, the humidity in the artificial climate incubator is kept between 75% and 85%, the illumination time is not less than 8h/d, and the illumination is 2000-3500 Lux.
Preferably, when the first true leaf grows out, the arch-shaped seal is made by binding wires according to the depth of the shallow tray, so that the seedling growth space is ensured.
The invention has the advantages that:
aiming at the problem of difficult germination of the rhododendron in Henan, the invention adopts the PEG-6000 solution with the content of 2.5 percent to promote the germination of the seeds in advance, the germination rate of the seeds can be improved by more than 80 percent, and the seeds are not susceptible to diseases, the discovery is obtained accidentally in the process of exploring the water demand of the germination process of the seeds, the PEG-6000 solution is generally adopted to simulate the response of the germination of the seeds to water stress, and the PEG-6000 solution can inhibit the germination of the seeds in general, but the invention discovers that the PEG-6000 solution with the content of 2.5 percent promotes the germination of the seeds in the experimental process of the aspect, therefore, in order to improve the germination rate of the rhododendron in Henan, the PEG-6000 solution with the content of 2.5 percent is adopted to treat the rhododendron in Henan, and the treatment has the technical effect of not being reserved.
The seedling raising method adopts a shallow tray matrix layering method, only adopts two types of matrixes, and has a loose and breathable matrix and a certain water-retaining effect as a bottom layer; the second substrate has certain air permeability, is used for seed germination and seedling raising, and a layer of pinhole preservative film or plastic film (or professional water permeable film) is paved between the two substrates, so that nutrient and moisture loss in the second substrate can be effectively prevented, and meanwhile, when the upper substrate is slightly drought, the moisture in the lower substrate plays a role in reverse osmosis through holes in the film, so that soil moisture is increased and preserved for the upper substrate, thereby reducing irrigation times and lowering maintenance cost. The seedling raising method is easy to operate, the seedling raising trays are various in variety and flexible in size, wastes such as snack boxes can be selected for recycling, the sprinkling frequency is reduced, water is saved, the maintenance cost is low, in addition, the seedling raising is not limited by time, and the emergence rate can reach more than 90%.
Drawings
FIG. 1 is a schematic illustration of a towel level wet hiding process;
FIG. 2 is germination of 2.5% PEG treated seeds;
FIG. 3 shows the growth of plug seedlings after 60 days and 80 days;
FIG. 4 shows the growth of a normal seedbed after 80 days;
FIG. 5 shows the growth and development of the layered seedling culture of the shallow tray substrate of the invention for 40 days;
FIG. 6 shows the growth and development of the layered seedling culture of the tray substrate of the invention for 60 days (the third cotyledon is grown);
FIG. 7 is the effect of PEG concentration on germination of Rhododendron henryi seeds;
FIG. 8 shows the seedling emergence rate of the shallow tray substrate layered seedling culture and sowing for 40 days;
FIG. 9 shows the 80-day emergence rate of the shallow tray substrate layered seedling culture and sowing of the invention.
Detailed Description
The invention will be further described with reference to specific embodiments, and advantages and features of the invention will become apparent from the description. However, unless otherwise indicated, all the specific examples described in the examples below were either conventional or were carried out under the conditions recommended by the manufacturer's instructions.
The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated. The test methods in the following examples are conventional methods unless otherwise specified. Unless otherwise indicated, all reagents and materials used are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the present invention. The preferred methods and materials described herein are presented for illustrative purposes only.
Example 1
A method suitable for germination accelerating and seedling raising of rhododendron in Henan comprises the following steps:
(1) Seed collection and treatment: the wild Henan azalea collected in the top of Henan Baiyun mountain Yuhuang in the late 10 months is taken, the capsules are brought back to the physiological laboratory of garden plants in the Henan Shangzi lake school area of the economic college of the animal industry, and are placed in a dry place for drying in the shade, and when the capsules are naturally cracked, the full seeds of the seeds are collected and the impurities are removed. The clean seeds were wrapped in layers with wet towels and kept in a refrigerator at 4 ℃ for later use, keeping the towels moist all the time (sand storage is not recommended because the seeds of rhododendron henryi are tiny) (fig. 1).
(2) Accelerating germination of seeds: uniformly spreading the treated Henan rhododendron seeds in a culture dish or shallow tray with three layers of filter paper, carrying out water treatment with PEG-6000 solution with the content of 2.5% (the corresponding solution water potential is-0.1 MPa), and carrying out germination in a constant-temperature artificial climate incubator with the temperature of 20 ℃ or a variable-temperature artificial climate incubator with the temperature of 20-25 ℃, keeping the humidity of 70-80%, replacing the filter paper every 3-4 days, and carrying out radicle peeling after about 2 weeks, namely, germination. Compared with the germination accelerating with clear water, the PEG-6000 solution with the concentration of 2.5 percent promotes the germination of seeds for 1 to 2 days in advance, improves the germination rate of the seeds, can reach more than 80 percent and is not easy to cause diseases. The hypocotyl continues to grow and can be planted after about one month.
(3) The shallow tray substrate layered seedling method comprises the following steps: (1) tray selection and substrate preparation: trays or other containers with a depth of 2-4cm were selected. Preparing two matrixes, wherein the first matrix is pine needle soil and turfy soil=3:1; the matrix II is turf, vermiculite, loose soil and perlite=2:2:1:1 (perlite can be reduced in amount). The substrate is sterilized at 120 ℃ for 20 minutes.
(2) Seedling time: the seedling raising in the Henan academy of economics, dragon lake school area belongs to the field seedling raising, so that the seed germination is started according to (2) in the middle ten days of 5 months, and the germination is completed for planting in about 6 ten days of the middle month.
(3) Shallow tray substrate layered seedling treatment: firstly, uniformly spreading the first matrix in the step (3) on the bottom of a shallow tray, wherein the thickness of the first matrix is about 1/4 of the depth of the shallow tray, and spraying a small amount of FeSO 4 And the pH of the purified water is 6.0-7.0, a layer of preservative film or plastic film with the thickness of 0.1-0.2mm is paved on the substrate, the preservative film is used for wrapping the bottom and the peripheral tray walls of the whole tray, then a plurality of small holes (the number of trays with the diameter of 10cm is determined according to the area size) are uniformly punched on the film by using needles, and the professional water permeable film can also be directly used. And then uniformly spreading a layer of matrix II on the preservative film, wherein the thickness of the matrix II is about 1/2 of the depth of the disc, and spraying purified water. Uniformly scattering the germinated seeds on a second substrate, covering a thin and screened second substrate, sealing the shallow tray opening by using a preservative film, placing the shallow tray opening in a constant-temperature artificial climate incubator at 20 ℃, keeping the humidity between 75% and 85%, and illuminating 2000-3500 Lux for at least 8h/d.
(4) Emergence and maintenance: the purified water is poured once in 4-5 days, the cotyledons are broken into earth and then are grown into first true leaves after about 10-15 days, and at the moment, arch seals can be made by binding wires according to the depth of the shallow tray, so that the seedling growth space is ensured. When 2-4 true leaves grow out in 8 months, the seal is partly uncovered in the early stage, and the ventilation seedling hardening time gradually increases from 1 hour per day to completely uncover the seal; and taking the shallow tray seedlings with the opened seals out of the artificial climate incubator for hardening seedlings after 1 month, and gradually increasing from 1 hour per day to completely adapt to the external environment. When the average gas temperature of the moving average is reduced to 16-26 ℃, the first time of rhododendron seedling transplanting can be carried out.
Experimental example
1 influence of different seedling raising modes on germination of rhododendron henryi seeds and growth of seedlings
1.1 plug seedling
Selecting a cave dish with proper aperture and proper hole depth from garden nursery greenhouse of Henan animal husbandry institute, placing the cave dish at a ventilation place under the sun for insolation for 24 hours, uniformly spraying 75% alcohol on the surface of the cave dish to disinfect the cave dish, washing the cave dish with clear water, and airing for later use. The matrix is a mixed matrix of turfy soil, vermiculite, pine needle soil and perlite=2:2:1:1.
1.2 seedling raising in seedbed
In a garden nursery greenhouse of the Henan academy of animal husbandry, a 0.5m 1m seedbed is made, and the matrix is a mixed matrix of turf, vermiculite, loose soil and perlite=2:2:1:1.
1.3 shallow tray substrate layered seedling
The process is carried out in a garden laboratory artificial climatic chamber of Henan animal husbandry, and 3 trays with the diameter of 20cm and the depth of 3cm are used. Preparing two matrixes, wherein the first matrix is pine needle soil and turfy soil=3:1; the matrix II is turf, vermiculite, loose soil and perlite=2:2:1:1 (perlite can be reduced in amount). Both the substrate and the seal were made of plastic film with a thickness of 0.2mm.
1.4 seed treatment modes
The collected seeds are respectively preserved in 4 storage modes, namely, low-temperature wet storage, low-temperature dry storage and normal-temperature dry storage. Wrapping seeds with wet towels and storing the seeds in a refrigerator at 4 ℃ by low-temperature wet storage, and keeping the towels wet; the low-temperature dry storage is to put seeds in a breathable cotton bag and store the seeds in a refrigerator at 4 ℃; dry storage at room temperature, namely, putting seeds into a breathable cotton bag and storing in a room temperature environment; the wet towel is wrapped in a wet towel at normal temperature and stored in a normal temperature environment. Taking out the seeds after the experiment, soaking the seeds for 24 hours with warm water at 50 ℃ and cleaning the seeds with distilled water for use.
1.5 germination acceleration treatment
To further explore the moisture demand of the seed germination process, the water treatment was simulated with PEG-6000 solution: PEG-6000 content was 2.5%, 5%, 10%, 20%, control was incubated with distilled water without PEG-6000 (0%), and the statistical germination rate was shown in FIG. 7.
As can be seen from FIG. 7, the germination rate was 50-60% in the case of the culture with distilled water without PEG-6000, but the germination rate was more than 80% after the treatment with the PEG-6000 solution having a content of 2.5%, and the germination rate was suppressed as the PEG-6000 content was gradually increased, and it was found that the germination rate was improved by the treatment with the PEG-6000 solution having a content of 2.5%, as shown in FIG. 2.
1.6 seedling test design (1.5 germination-accelerating seeds are adopted in the test)
The plug seedling takes 10 holes as a group, and 10 rhododendron seeds are sown in each hole; the seedling raising method adopts slice broadcasting, each treatment is provided with 3 repeats, and each repeat requires 100 seeds stored in 4 modes. The shallow tray substrate layered seedling method was repeated with 3 trays, each tray being sown with 100 seeds (seeds as above). Sowing at 2021, 3 and 23 days, and irrigating each day with FeSO solution according to dry and wet state of culture medium 4 And the purified water with pH value of 5.5-6.0 is covered with plastic film with thickness of 0.2mm for moisturizing. Recording the number of seedlings after sowing for 40 days, and calculating the germination rate (figure 8); the number of seedlings was recorded after 80 days of sowing, and the survival rate was calculated (FIG. 9).
Fig. 3 shows the growth conditions after 60 days and 80 days of plug seedling, fig. 4 shows the growth condition after 80 days of ordinary seedling bed seedling, and fig. 5 shows the germination condition after 40 days of shallow matrix layered seedling, and compared with the conventional seedling bed seedling and plug seedling, as can be seen from fig. 8 and 9, the germination rate of the shallow matrix layered seedling of the invention is greatly improved, and the survival rate of the shallow matrix layered seedling of the invention is greatly improved, namely, the germination rate of the shallow matrix layered seedling is greatly improved, namely, the survival rate of the shallow matrix layered seedling is greatly improved.
The above-mentioned embodiments are merely preferred embodiments of the present invention, which are not intended to limit the scope of the present invention, and other embodiments can be easily made by those skilled in the art through substitution or modification according to the technical disclosure in the present specification, so that all changes and modifications made in the principle of the present invention shall be included in the scope of the present invention.
Claims (8)
1. A method suitable for germination accelerating and seedling raising of rhododendron in Henan, which is characterized by comprising the following steps:
seed treatment: drying collected wild Henan azalea capsules in the shade at a drying place, collecting seeds with full seeds and removing impurities when the capsules are naturally cracked, wrapping clean seeds in layers by wet towels, and storing the seeds in a refrigerator at a low temperature of 4 ℃ for standby, wherein the towels are kept moist all the time;
accelerating germination of seeds: uniformly spreading the rhododendron heyneanum seeds treated in the step (1) in a culture dish or shallow dish with three layers of filter paper, carrying out water treatment by using a PEG-6000 solution with the concentration of 2.5% until radicle is broken and out, specifically, carrying out germination acceleration by placing the rhododendron heyneanum seeds in a constant-temperature artificial climate incubator with the temperature of 20 ℃, keeping the humidity of 70% -80%, and replacing the filter paper every 3-4 days, wherein the water potential of the PEG-6000 solution is-0.1 MPa;
(3) Shallow tray substrate layered seedling raising: selecting a shallow tray with the depth of 2-4cm, preparing two matrixes, namely a matrix I and a matrix II, uniformly spreading the matrix I on the bottom of the shallow tray, spraying purified water with the pH value of 6.0-7.0, spreading a layer of preservative film or plastic film on the matrix I, wrapping the bottom and the peripheral tray walls of the whole shallow tray by the preservative film or plastic film, and uniformly puncturing small holes on the preservative film or plastic film by using needles; uniformly spreading a layer of matrix II on the preservative film, spraying purified water, uniformly scattering the germinated seeds on the matrix II, covering a layer of thin and screened matrix II, sealing the shallow tray opening by the preservative film, and culturing in a constant-temperature artificial climate incubator at 20 ℃; the first matrix is prepared by mixing the pine needle soil and the turfy soil according to the weight ratio of 3:1, and the second matrix is prepared by mixing the turfy, vermiculite, the pine needle soil and perlite according to the weight ratio of 2:2:1:1; the thickness of the first substrate laid on the shallow tray is 1/4 of the depth of the shallow tray, and the thickness of the first substrate laid on the shallow tray is 1/2 of the depth of the shallow tray;
(4) Emergence and maintenance: pouring purified water once in 4-5 days, breaking soil after 8-10 days, growing first true leaves after 10-15 days, and when 2-4 true leaves grow in 8 months, half opening the seal in the early stage, and gradually increasing the ventilation seedling hardening time from 1 hour per day to completely opening the seal; and taking the shallow tray seedlings with the uncovered seals out of the artificial climate incubator for hardening off after 1 month, gradually increasing from 1 hour per day to completely adapt to the external environment, and transplanting the rhododendron seedlings for the first time when the average gas temperature of the moving average is reduced to 16-26 ℃.
2. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the constant temperature artificial climate incubator at 20 ℃ is replaced by a variable temperature artificial climate incubator at 20-25 ℃.
3. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the two substrates are sterilized at 120 ℃ for 20 minutes.
4. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the thickness of the preservative film or plastic film is 0.1-0.2mm.
5. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the number of the small holes is 0.5-1/cm 2 。
6. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the preservative film or plastic film is replaced by a professional water permeable film.
7. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein the humidity in the artificial climate incubator is kept between 75% and 85%, the illumination time is not less than 8h/d, and the illumination is 2000-3500 Lux.
8. The method for accelerating germination and seedling raising of rhododendron in accordance with claim 1, wherein when the first true leaves are grown, arch-shaped seals are made by binding wires according to the depth of the tray, thereby ensuring the seedling growth space.
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| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
| CN110178689A (en) * | 2019-06-06 | 2019-08-30 | 贵州省农科院山茂园艺工程技术有限公司 | A kind of preparation method of base material of tissue-cultured derived plant lily hardening |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
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| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
| CN110178689A (en) * | 2019-06-06 | 2019-08-30 | 贵州省农科院山茂园艺工程技术有限公司 | A kind of preparation method of base material of tissue-cultured derived plant lily hardening |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
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