CN102771292B - Industrial piptanthus nepalensis f.leiocarpus seedling rearing method - Google Patents
Industrial piptanthus nepalensis f.leiocarpus seedling rearing method Download PDFInfo
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Abstract
The invention discloses an industrial piptanthus nepalensis f.leiocarpus seedling rearing method. A seed germination method provided by the invention comprises variable temperature imbibition, acid etching with 98% concentrated sulfuric acid, seed soaking in high-temperature water and constant temperature accelerating germination. The industrial seedling rearing method provided by the invention comprises a method for comprehensively regulating and managing such cultivation environment conditions as sowing and covering depth, cultivation substrate ratio, illumination, moisture and pH value. By utilizing the industrial seedling rearing technology provided by the invention, the germination rate of piptanthus nepalensis f.leiocarpus seeds can be improved by 80%, the seedling rate can be improved by over 60% and the seedling rearing time is shortened by 90 days. The method provides feasible key technology for industrial piptanthus nepalensis f.leiocarpus seedling rearing.
Description
Technical field
The present invention relates to a kind of method of light fruit greenleaf piptanthus seed factorial seedling growth.
Background technology
Light fruit greenleaf piptanthus seed (Piptanthus nepalensis f.leiocarpus), pulse family, greenleaf piptanthus seed belong to, the modification of naple yellow flowers and trees, evergreen shrubs, distribution Tibet, Yunnan, Qinghai, Nepal; The Qinghai-Tibet Platean endemic plant.This kind happy expression is waited gentle, is distributed in the sand of well-drained hillside happiness illumination, impoverishment tolerant more; Plant is crown plentiful, and is with luxuriant foliage and spreading branches in leafy profusion dark green, and the bright Huang of pattern is beautiful; Contain nutrient components such as rich in protein, fat, amino acid in branches and leaves and the seed.Flourishing because of it, the four seasons are evergreen, leaf unusual, flower amount is various, has higher ornamental value; Seed is nutritious, and the output height has potential feeding value; Simultaneous adaptation is extensive, growth is rapid, has the ecotope reparation and is worth.
But light fruit greenleaf piptanthus seed distributes remote, and domestic no artificial cultivating and growing also only has species to preserve abroad in the only a few botanical garden.
Use conventional method and carry out light fruit greenleaf piptanthus seed planting seed and grow seedlings, emergence rate less than 5% usually, and the seeding stage is long, is difficult to obtain the seedlings of a large amount of neat stalwartnesses, and reproductive efficiency is very low.Few about the research report of greenleaf piptanthus seed platymiscium aspect both at home and abroad, blank at present about the research of seed seedling-raising aspect both at home and abroad.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of method that makes light fruit greenleaf piptanthus seed (Piptanthus nepalensis f.leiocarpus) seed factorial seedling growth.
Provided by the present invention is the method for light fruit greenleaf piptanthus seed (Piptanthus nepalensis f.leiocarpus) factorial seedling growth, comprises the steps:
(1) light fruit greenleaf piptanthus seed seed normal temperature clear water was soaked 1 day, obtain the light fruit greenleaf piptanthus seed seed of imbibition; Described soak at room temperature is: it is in 15 ℃～25 ℃ the water that light fruit greenleaf piptanthus seed seed is soaked in room temperature; Will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed be for further processing;
(2) will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed carry out alternating temperature and soaked 2 days～4 days, obtain the light fruit greenleaf piptanthus seed seed of imbibition; Described alternating temperature soaks: will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed to be soaked in initial temperature be in 80 ℃～100 ℃ the water 2 days～4 days, taking out every day and changing initial temperature behind the sucting expansion seed is 80 ℃～100 ℃ water, and water temperature is dropped to below 25 ℃ naturally; Will be after step (2) be handled not the light of imbibition fruit greenleaf piptanthus seed seed be for further processing;
(3) with described after step (2) is handled not the light of imbibition fruit greenleaf piptanthus seed seed soaked 40 minutes～60 minutes with the concentrated sulfuric acid, obtain the light fruit greenleaf piptanthus seed seed of acid etching;
(4) the light fruit greenleaf piptanthus seed seed clear water of described acid etching is cleaned back high-temperature water seed soaking 1 day～3 days, obtain the light fruit greenleaf piptanthus seed seed of imbibition; The seed soaking of described high-temperature water is: be soaked in initial temperature after the light fruit greenleaf piptanthus seed seed clear water of described acid etching is cleaned and be in 80 ℃～100 ℃ the water, water temperature is dropped to below 25 ℃ naturally, and take out that to change initial temperature behind the sucting expansion seed be 80 ℃～100 ℃ water every day;
(5) light of the imbibition that described step (1), (2), (4) are obtained fruit greenleaf piptanthus seed seed carried out vernalization 3 days～8 days under temperature is 20 ℃～25 ℃, dark and moistening condition, namely obtained the light fruit greenleaf piptanthus seed seed of sprouting;
(6) light of the sprouting that described step (5) is obtained fruit greenleaf piptanthus seed seed is in the canopy indoor seeding, sowing is in the mixed-matrix of turfy soil and river sand, the volume ratio that turfy soil accounts for matrix is 50～85%, 1 centimetre of thickness of earth-fill cover, in full exposure or 33% following shading, matrix relative water content 75～90%, carry out the factorial seedling growth management of seedling under the environmental condition of pH value 6～8, when growing to 2-3 sheet true leaf, seedling carried out hardening 5-8 days, make seedling adapt to external environmental condition gradually by the air port of regulating the canopy chamber, when the outdoor day minimum air temperature stablized above 12 ℃, daily mean temperature goes out canopy and transplants when being stabilized in 20-25 ℃.
It is preceding that described method also is included in described step (1), removes impurity and empty flat, worm-eaten seed; The shady and cool drying of back placement of drying in the shade is located or places under 0～5 ℃ of refrigerated condition to preserve, and described light fruit greenleaf piptanthus seed seed is carried out threshing and clean kind processing as follows: the fruit pod of being exposed to the sun makes it breach, the taking-up seed.
Described not imbibition light fruit greenleaf piptanthus seed seed keeps the constant seed of original morphological feature for handling the back through seed soaking.
Before described step (6) sowing, utilize 800-1000 broad-spectrum germicide doubly to spray matrix it is carried out disinfection.
The invention has the beneficial effects as follows: reported first of the present invention the factorial seedling-culturing method of light fruit greenleaf piptanthus seed, creatively sum up a whole set of factorial seedling-culturing method, concrete advantage is as follows:
(1) after step (1) normal temperature seed soaking, complete ripeness or seed imbibition damaged but that do not influence vigor are not sprouted, thereby improve the service efficiency of seed;
(2) by high-temperature water seed soaking in 2～4 days, make incomplete hardseed guarantee fully imbibition under the prerequisite of seed quality, thereby improve the germination rate of seed;
(3) acid etching can be improved the imbibition rate of hardseed and be sprouted speed, shortening seed imbibition time, makes the whole germination process of seed more concentrated;
(4) constant temperature vernalization is sprouted, and improves sprouting speed, and makes seed germination rate surpass 90% at short notice, significantly shortens the time that reaches maximum germination rate, makes the seedling neat and consistent;
(5) the present invention determines planting environment conditions such as illumination, moisture and acid-base value in optimum sowing depth and cultivation medium formula, the factorial seedling growth through a large amount of tests, and sum up and be fit to light fruit greenleaf piptanthus seed and go out the optimum condition of canopy open country field planting, through above-mentioned comprehensive regulation, can obviously improve survival rate, the promotion seedling growth of seedling, what assurance was grown seedlings is neat, healthy and strong with consistent.
Utilize the present invention to promote the method for light fruit greenleaf piptanthus seed seed germination higher by 85% than direct insemination and emergence rate, planting percent is high by 70%, than sand hide after 1 month～4 months the insemination and emergence rate high about 80%, planting percent is high by 65%; The insemination and emergence time shortens 90 days (not containing the husky Tibetan time); Reasonably sowing thickness of earth-fill cover, cultivation matrix proportioning and useful planting environment factor regulation and control can ensure quality, quantity and the production efficiency of light fruit greenleaf piptanthus seed factorial seedling growth.
Description of drawings
Fig. 1 is that different water temperatures are to the influence of the fresh light fruit greenleaf piptanthus seed seed imbibition process of different store methods.
Fig. 2 is that different water temperatures are to the influence of the light fruit greenleaf piptanthus seed seed imbibition process of different store method storages after 1 year.
Fig. 3 is imbibition rate and the germination rate of 98% concentrated sulfuric acid different time acid etching light fruit greenleaf piptanthus seed seed.
Fig. 4 is that temperature is to the influence of light fruit greenleaf piptanthus seed seed germination.
Fig. 5 is for using the process of emerging of the present invention and conventional method sowing light fruit greenleaf piptanthus seed seed.
Fig. 6 compares for the planting percent of using the present invention and conventional method sowing light fruit greenleaf piptanthus seed seed.
Fig. 7 is the influence of light fruit greenleaf piptanthus seed factorial seedling growth sowing depth to the process of emerging and seedling plant height.
Fig. 8 is that the batch production of light fruit greenleaf piptanthus seed is produced seedling medium to the influence of survival rate and plant height growth.
Fig. 9 is that light fruit greenleaf piptanthus seed factorial seedling growth envirment factor is to the influence of survival rate and plant height growth.
Embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Light fruit greenleaf piptanthus seed seed used among the following embodiment all picks up from Tibet, and Total Test carries out in Institute of Botany, Chinese Academy of Sciences botanical garden (No. 20, Haidian District Beijing Fragrance Hill, address Nan Xincun), and test period is from year August in January, 2009 to 2012.
The assay method of described imbibition rate is: randomly draw some light fruit greenleaf piptanthus seed seed; Normal-temperature water seed soaking in first day, change initial temperature subsequently every day and be the clear water seed soaking of 15～25 ℃, 50 ℃～60 ℃ and 80 ℃～100 ℃ of temperature, make water temperature drop to room temperature naturally, soaked seed altogether 10 days, before changing the different temperatures clear water sucting expansion seed is picked, record sucting expansion seed number every day, calculate the imbibition rate of different water temperatures with following formula:
The computational methods of hard seed rate are:
Hard seed rate (%)=1-imbibition rate
One, different water temperature seed soaking are to the influence of light fruit greenleaf piptanthus seed seed imbibition and the process of sprouting
Choose fresh seeds that normal temperature and low temperature preserves and air drying and the low temperature greenleaf piptanthus seed seed in 1 year of storage of preserving moisture, with above-mentioned imbibition rate assay method the light of different store methods and holding time fruit greenleaf piptanthus seed seed is carried out imbibition, observation imbibition process, calculate imbibition rate and hard seed rate; The results are shown in Figure 1, Fig. 2.
The result shows: adopting that the back preserves at normal temperature condition under 5 days the water temperature condition of fresh seeds at all temps nearly all can imbibition on the same day, no strong; But 10 days imbibition rate is 30% to fresh seeds under normal temperature condition, hard seed rate reaches 70% after through 5 days deepfreeze, 10 days imbibition rates of 50 ℃～60 ℃ of water seed soakings are 49%, 10 days imbibition rate of 80 ℃～100 ℃ high-temperature water imbibitions surpasses 90% (Fig. 1), illustrate that the new seed of gathering does not have strong phenomenon, deepfreeze can cause seed strong.
Seed seed imbibition process, imbibition rate and the germination rate etc. under the water temperature condition of all temps of preserving 1 year under air drying and 0 ℃～5 ℃ refrigerated conditions are obvious according to the different differences of water temperature, but it is irrelevant with store method, two kinds of store method normal temperature or 10 days imbibition rate of hot water imbibition 10d imbibition rate 30%, 80 ℃～100 ℃ high-temperature water imbibitions of equal less than reach 99.5% (Fig. 2).
Above-mentioned experimental result shows: the new seed of gathering can directly be sowed after threshing, the clean kind, and deepfreeze or long term storage all can cause seed strong; Seed soaking optimization process method be first day with normal-temperature water seed soaking 24 hours, separate sucting expansion seed subsequently every day after, change 80 ℃～100 ℃ high-temperature water seed soaking, make water temperature be down to room temperature naturally.
Two, the different acid etching times are to the influence of light fruit greenleaf piptanthus seed seed imbibition and sprouting
Be that 98% concentrated sulfuric acid soaks respectively through the light of low temperature storage fruit greenleaf piptanthus seed seed 0min (ck), 20min, 40min, 60min with mass percent concentration, measure 10 days high temperature imbibition rate of seed and 25 ℃ of constant temperature germination rates under the different disposal condition respectively,
The results are shown in Figure 3.
The result shows: the optimal acid etching method of strong light fruit greenleaf piptanthus seed seed of preserving through low temperature is 98% concentrated sulfuric acid acid etching 40 minutes～60 minutes, handles two days later that seed imbibition rate can reach more than 95%, and final germination rate surpasses 95%; The seed imbibition rate of handling without 98% concentrated sulfuric acid only is 6.56%, germination rate is 6.13%.
Three, temperature is to the influence of light fruit greenleaf piptanthus seed seed germination
Will through the light of low temperature storage fruit greenleaf piptanthus seed seed carry out acid etching of the present invention, imbibition, seed soaking handle after respectively 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ constant temperature and 15 ℃/25 ℃ and 20 ℃/30 ℃ 12 hours the daytime/carry out sprouting test under the alternating temperature condition, the results are shown in Figure 4 night.
Fig. 4 shows: the suitable sprouting temperature of light fruit greenleaf piptanthus seed is 20～30 ℃ of constant temperature, 15 ℃/25 ℃ and 30 ℃/20 ℃ alternating temperatures, and general the 2nd, 3 day begins to sprout, and germination rate all can reach maximum in 10～12 days; But it is poor that the sprouting process that alternating temperature is handled and final germination rate are obviously handled than constant temperature.20～25 ℃ of constant temperature seed germinations are the fastest, 6～8 days germination rates surpass 8 days germination rate of 90%, 20 ℃ of constant temperature seed and reach 95%, reach 14～16 days the highest germination rate time.10 ℃ and 15 ℃ of constant temperature began sprout time the 5th～6 day, but at 16～18 days germination rates all above 90%, reach maximum, illustrate that the sucting expansion seed of light fruit greenleaf piptanthus seed also can guarantee higher vigor under lower temperature condition.35 ℃ the highest germination rate only is 51.67%; 35 ℃ of temperatures involved light fruit greenleaf piptanthus seed seed germinations are described.
Therefore, with reference to above-mentioned experimental result, think when upper soll layer (in open country or the facility) daily mean temperature is lower than 25 ℃ above 10 ℃, after application the technology of the present invention is carried out strong processing, imbibition to light fruit greenleaf piptanthus seed seed, get final product direct seeding and seedling raising, the optimum season of general outdoor sowing is that spring end is to early summer.
The contrast experiment that embodiment 2, light fruit greenleaf piptanthus seed seed conventional seed planting and the present invention grow seedlings
The light fruit greenleaf piptanthus seed seed of gathering in October, 2009 is selected in this experiment for use.Conventional method threshing, the clean kind are packed into and are sealed plastic sack in the refrigeration of preserving moisture of 0 ℃～5 ℃ refrigerators, and 400 seeds are selected in every processing at random for use, divide 4 repetitions, 100 of every repetitions; The clear water seed soaking is 48 hours before husky the Tibetan, uses long-pending 0,1,2,3,4 month of wet sand bed respectively; On February 28th, 2011 was sowed in nothing is heated glass greenhouse simultaneously.The inventive method in contrast, clear water seed soaking on March 11 day, 2～March 6 March, 80～100 ℃ of high-temperature water were down to room temperature seed soaking 5 days, March 6 98% concentrated sulfuric acid acid etching 60 minutes naturally, clear water is cleaned, and is down to room temperature seed soaking 3 days naturally with 80～100 ℃ of high-temperature water again; Take out sucting expansion seed to place under 10 ℃ of cryogenic conditions and preserve every day, vernalization in 20 ℃ of constant incubators of beginning on March 4, when treating radicle growth to 2～3 centimetre, it is planted in river sand and 1: 3 the matrix container of turfy soil volume ratio, 12 little time in 20 ℃ of constant incubators/dark grow seedlings in alternately, divide March 19 to plant under the equivalent environment of above-mentioned sowing and plant seedling.Statistics was respectively handled the number of emerging in per 5 days, and the final number of seedling of statistics is calculated emergence rate and planting percent (Fig. 5, Fig. 6) after 100 days.
Comparing result shows: long-pending 1～4 month method for treating seeds of conventional wet sand bed began to emerge in after planting 6 days～10 days, reached maximum emergence rate (Fig. 6) in 80 days～100 days, the present invention since March 1 handle to March 19 the bu seedling less than reaching maximum emergence rate in 20 days.About 15% (being up to husky the Tibetan 1 month 16.75% and 15.0%) of conventional method for treating seeds long-pending 1～4 month emergence rate of wet sand bed and planting percent, obvious emergence rate and planting percent 8.75% and 8.5% height than directly sowing processing, but the processing differences of different lamination times is not remarkable; And the highest emergence rate of the present invention planting percent 80% after 95.5%, 100 day, apparently higher than conventional method, the time that reaches maximum germination rate only is 8 days, true leaf opens 20 days time, shortens about 80 days (not containing the husky Tibetan time) than conventional method seeding and seedling raising.
The seedling of the neat and consistent of present technique sprouting is selected in the experiment of factorial seedling growth sowing depth for use, in the cultivation matrix of the uniformity of unified preparation, adopt 3 processing such as 1 centimetre of thickness of earth-fill cover, 2 centimetres, 3 centimetres to compare experiment respectively, 30 seed, 3 repetitions of sprouting the same day of every processing, the observed and recorded quantity of emerging every day, measure the seedling plant height after 30 days, calculate emergence rate and survival rate, the results are shown in Figure 7.The left figure of Fig. 7 shows in the sowing 30 days the different sowing depth situation of emerging, and 1 centimetre, 2 centimetres, 3 centimetres of sowing depth seedling-growing time first were respectively 6 days, 8 days and 18 days; The time that reaches maximum emergence rate was respectively 17 days, 26 days, 28 days, maximum emergence rate is respectively 90.83%, 77.5%, 59.17%, planting percent is respectively 90.83%, 73.33% and 59.17, seedling height is respectively 5.45 centimetres, 5.44 centimetres and 6.11 centimetres, the seedling plant height of 3 centimetres of processing of earthing is apparently higher than the shallow processing of earthing, but seedling is very weak, illustrates that the best thickness of earth-fill cover of light fruit greenleaf piptanthus seed sowing is 1 centimetre.
The factorial seedling growth cultivation experiments selects for use neatly, healthy and strong, consistent 2 years living seedling of light fruit greenleaf piptanthus seed, compare test in the acid-base value to its cultivation matrix kind, proportioning, illumination, water supply in media and irrigation water between 100 days of in April, 2010 to August, its mesostroma 1～6 represents respectively: matrix 1 perlite; Matrix 2 river sands; Matrix 3 river sands: turfy soil=3: 1; Matrix 4 river sands: turfy soil=1: 1; Matrix 5 river sands: turfy soil=1: 3; Matrix 6 turfy soils.Light 1～3 represents respectively: light 1 shading rate 67%; Light 2 shading rates 33%; Light 3 full exposures.Water 1～4 represents respectively: 25%, 50%, 75%, 100% of matrix maximum water-holding capacity.The results are shown in Figure 8, Fig. 9.Fig. 8 shows that 6 kinds of different cultivation matrix proportionings are to the influence of light fruit greenleaf piptanthus seed survival rate and plant height growth, the survival rate of matrix 4, matrix 5 and matrix 6 and plant height growth obviously are better than other 3 kinds of substrate compositions, the survival rate of its mesostroma 5 is the highest, the growth of the plant height of matrix 4 is maximum, illustrate that light fruit greenleaf piptanthus seed factorial seedling growth cultivation matrix is suitable for selecting the high mixture ratio mixed-matrix of turfy soil and sand, the accommodation of turfy soil proportioning is 50～85% of volume ratio.Fig. 9 shows that light fruit greenleaf piptanthus seed growth differences is very big under different illumination conditions, and suitable illumination amplitude is full light to 33% shading, and the survival rate of full exposure and 33% shading treatment is higher than 67% shading treatment, and plant height is 1.53 times of 67% shading treatment; Light fruit greenleaf piptanthus seed growth of seedling is very high to the requirement of moisture, control water test mesostroma maximum water-holding capacity does not also drop at 50% o'clock, seedling is namely all dead, reproducible results is identical several times, show that light fruit greenleaf piptanthus seed should keep sufficient moisture when growing seedlings, but cultivation matrix can not be in the water saturation state all the time, and it is high by 10% that the processing of maximum water-holding capacity 75% is processed into motility rate than 100%, and plant height is grown to its 1.4 times; Irrigation water acid-base value comparative trial is to be that 5,6,7,8,9 different moisture are irrigated light fruit greenleaf piptanthus seed with the pH value at every turn, the result shows that this kind is wider to the accommodation of pH value, but equal normal growth between the pH value 6～8, the pH value is that 7 o'clock survival rates are 95%, plant height is higher than other at least and handles 1.23 times, so light fruit greenleaf piptanthus seed is cultivated optimal acid-base value for neutral.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (4)
1. the method for light fruit greenleaf piptanthus seed (Piptanthus nepalensis f.leiocarpus) factorial seedling growth comprises the steps:
(1) light fruit greenleaf piptanthus seed seed normal temperature clear water was soaked 1 day, obtain the light fruit greenleaf piptanthus seed seed of imbibition; Described soak at room temperature is: it is in 15 ℃～25 ℃ the water that light fruit greenleaf piptanthus seed seed is soaked in room temperature; Will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed be for further processing;
(2) will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed carry out alternating temperature and soaked 2 days～4 days, obtain the light fruit greenleaf piptanthus seed seed of imbibition; Described alternating temperature soaks: will be after step (1) be handled not the light of imbibition fruit greenleaf piptanthus seed seed to be soaked in initial temperature be in 80 ℃～100 ℃ the water 2 days～4 days, taking out every day and changing initial temperature behind the sucting expansion seed is 80 ℃～100 ℃ water, and water temperature is dropped to below 25 ℃ naturally; Will be after step (2) be handled not the light of imbibition fruit greenleaf piptanthus seed seed be for further processing; (3) with described after step (2) is handled not the light of imbibition fruit greenleaf piptanthus seed seed soaked 40 minutes～60 minutes with the concentrated sulfuric acid, obtain the light fruit greenleaf piptanthus seed seed of acid etching;
(4) the light fruit greenleaf piptanthus seed seed clear water of described acid etching is cleaned back high-temperature water seed soaking 1 day～3 days, obtain the light fruit greenleaf piptanthus seed seed of imbibition; The seed soaking of described high-temperature water is: be soaked in initial temperature after the light fruit greenleaf piptanthus seed seed clear water of described acid etching is cleaned and be in 80 ℃～100 ℃ the water, water temperature is dropped to below 25 ℃ naturally, and take out that to change initial temperature behind the sucting expansion seed be 80 ℃～100 ℃ water every day;
(5) light of the imbibition that described step (1), (2), (4) are obtained fruit greenleaf piptanthus seed seed carried out vernalization 3 days～8 days under temperature is 20 ℃～25 ℃, dark and moistening condition, namely obtained the light fruit greenleaf piptanthus seed seed of sprouting;
(6) light of the sprouting that described step (5) is obtained fruit greenleaf piptanthus seed seed is in the canopy indoor seeding, sowing is in the mixed-matrix of turfy soil and river sand, the volume ratio that turfy soil accounts for matrix is 50～85%, 1 centimetre of thickness of earth-fill cover, in full exposure or 33% following shading, matrix relative water content 75～90%, carry out the factorial seedling growth management of seedling under the environmental condition of pH value 6～8, when growing to 2-3 sheet true leaf, seedling carried out hardening 5-8 days, make seedling adapt to external environmental condition gradually by the air port of regulating the canopy chamber, when the outdoor day minimum air temperature stablized above 12 ℃, daily mean temperature goes out canopy and transplants when being stabilized in 20-25 ℃.
2. method according to claim 1, it is characterized in that: described method is further comprising the steps of:
It is preceding that described method also is included in described step (1), removes impurity and empty flat, worm-eaten seed; The shady and cool drying of back placement of drying in the shade is located or places under 0～5 ℃ of refrigerated condition to preserve, and described light fruit greenleaf piptanthus seed seed is carried out threshing and clean kind processing as follows: the fruit pod of being exposed to the sun makes it breach, the taking-up seed.
3. method according to claim 1 is characterized in that: described not imbibition light fruit greenleaf piptanthus seed seed keeps the constant seed of original morphological feature for handling the back through seed soaking.
4. method according to claim 1 is characterized in that: before described step (6) sowing, utilize 800-1000 broad-spectrum germicide doubly to spray matrix it is carried out disinfection.
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| CN105144904A (en) * | 2015-07-09 | 2015-12-16 | 常州艾瑞特电子有限公司 | Method for promoting piptanthus tomentosus seed germination |
| CN108156880A (en) * | 2018-01-11 | 2018-06-15 | 四川农业大学 | A kind of method for culturing seedlings for improving greenleaf piptanthus seed seeding ratio |
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