CN107484462A - A kind of pre-sowing treatment method of sealwort seed - Google Patents
A kind of pre-sowing treatment method of sealwort seed Download PDFInfo
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- CN107484462A CN107484462A CN201710878645.9A CN201710878645A CN107484462A CN 107484462 A CN107484462 A CN 107484462A CN 201710878645 A CN201710878645 A CN 201710878645A CN 107484462 A CN107484462 A CN 107484462A
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- seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention mainly relates to Chinese medicine plant technical field, discloses a kind of pre-sowing treatment method of sealwort seed, including：Immersion, freezing, enzymolysis, vernalization, sowing；Method is simple, easy to operation, seed germinating time after planting is shorten to 17 ~ 19 days, germination percentage reaches 95.4%, hence it is evident that improves the plantation efficiency of sealwort；Seed is placed in progress high temperature immersion in the copper-bath containing sodium selenite, accelerates kind of soft and soggyization, kills the disease pest that retains in seed, promotes sodium selenite and copper sulphate to penetrate into seed, reduces dormancy species activity in seed, accelerate germination；Seed is freeze-dried after immersion, dormancy species activity activity is reduced, seed is quickly received high temperature and low temperature stimulation, the extraneous high/low temperature change being after planting subjected to is simulated, improves the germinating capacity of seed, accelerate germination, improve germination percentage.
Description
Technical field
The invention mainly relates to Chinese medicine plant technical field, more particularly to a kind of pre-sowing treatment method of sealwort seed.
Background technology
Sealwort, also known as polygonatum sibiricum Redoute, yellow chicken dish, pipe dish, claw ginseng, tendrilleaf solomonseal rhizome, achickenclaw ginseng, medicinal plant, with rhizome
Be used as medicine, can anti-inflammation, antiviral, hemostasis and pain-relieving, protection cardiovascular and cerebrovascular, balance blood sugar, moisten the lung and relieve the cough, be antifatigue, antioxygen
Change, anti-aging, but sealwort mainly still carry out it is wild, artificial growth it is few, a small number of artificial growths are still through asexual numerous
Grow, easily make the trait depression of sealwort, but when sealwort carries out zoogamy using seed, could germinate within after planting 2 ~ 3 years,
It could harvest within 4 ~ 5 years, and germination percentage can only achieve 60% or so, seriously reduce the plantation efficiency of sealwort.
The content of the invention
The defects of in order to make up prior art, it is an object of the invention to provide a kind of pre-sowing treatment method of sealwort seed.
A kind of pre-sowing treatment method of sealwort seed, comprises the following steps：
（1）Immersion：Sealwort seed that is ripe, complete, newly harvesting is selected, is placed in the copper-bath that mass concentration is 0.6 ~ 0.8%
In, soaked 3 ~ 5 hours in 40 ~ 45 DEG C, accelerate kind of soft and soggyization, killed the disease pest that retains in seed, promote sodium selenite and sulfuric acid
Copper penetrates into seed, reduces dormancy species activity in seed, accelerates germination, draining, obtains immersion seed；
（2）Freezing：Immersion seed is placed in freeze drier, is freeze-dried 5 ~ 7 hours, reduces dormancy species activity activity,
Seed is quickly received high temperature and low temperature stimulation, simulate the extraneous high/low temperature change being after planting subjected to, improve the germination energy of seed
Power, accelerate germination, improve germination percentage, obtain freezing seed；
（3）Enzymolysis：Freezing seed is placed in enzymolysis liquid, digested 2 ~ 3 hours in 40 ~ 42 DEG C, the institutional framework of kind of skin is destroyed, adds
External nourishment composition immerses seed soon, there is provided the nutriment needed for germination, accelerates plumule growth, filtering, must digest kind
Son；
（4）Vernalization：Enzymolysis seed being placed in germinating box, 1 ~ 3 day, regulation temperature is 24 ~ 25 DEG C, humidity is 76 ~ 78%, dark,
4 ~ 6 days, regulation temperature was 22 ~ 23 DEG C, humidity is that 81 ~ 83%, illumination is 80 ~ 100lx, first hot and humid promotion plumule growth, then
Dim light illumination is carried out, temperature is reduced, makes plumule gradually sturdy, strengthens resistance, makes after planting fast germination, improves germination percentage,
Obtain vernalization seed；
（5）Sowing：Vernalization seed is conventionally sowed, obtains sowing seed.
The step（1）Copper-bath, also containing mass concentration be 0.03 ~ 0.05% sodium selenite.
The step（2）Freeze-drying, temperature be -30 ~ -28 DEG C.
The step（3）Enzymolysis liquid, be made up of the enzyme preparation of following parts by weight：Cellulase 16 ~ 18, hemicellulase
11 ~ 13, lignoenzyme 8 ~ 10, lipase 3 ~ 5, pectase 3 ~ 5, all enzyme preparations are mixed, and add water to adjust to the matter of enzymolysis liquid
It is 0.03 ~ 0.05% to measure concentration.
It is an advantage of the invention that：The pre-sowing treatment method of sealwort seed provided by the invention, method is simple, easy to operation,
Seed germinating time after planting is set to shorten to 17 ~ 19 days, germination percentage reaches 95.4%, hence it is evident that improves the plantation efficiency of sealwort；Will
Seed is placed in progress high temperature immersion in the copper-bath containing sodium selenite, accelerates kind of soft and soggyization, kills retaining in seed
Disease pest, promote sodium selenite and copper sulphate to penetrate into seed, reduce dormancy species activity in seed, accelerate germination；After immersion
Seed is freeze-dried, reduces dormancy species activity activity, seed is quickly received high temperature and low temperature stimulation, simulation sowing
Change by the extraneous high/low temperature received, improve the germinating capacity of seed, accelerate germination, improve germination percentage；After freeze-drying
Seed is digested, destroys the institutional framework of kind of skin, accelerates external nourishment composition and immerses seed, there is provided needed for germination
Nutriment, accelerate plumule growth；Seed is placed in germinating box after enzymolysis, first hot and humid promotion plumule growth, then carry out weak
Light illumination, temperature is reduced, makes plumule gradually sturdy, strengthen resistance, make after planting fast germination, improve germination percentage.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of pre-sowing treatment method of sealwort seed, comprises the following steps：
（1）Immersion：Sealwort seed that is ripe, complete, newly harvesting is selected, is placed in the copper-bath that mass concentration is 0.6%,
Soaked 3 hours in 40 DEG C, quickening kind soft and soggyization, kill the disease pest that retains in seed, promote sodium selenite and copper sulphate to penetrate into kind
Son, dormancy species activity in seed is reduced, accelerate germination, draining, obtain immersion seed；Described copper-bath, also contains
There is the sodium selenite that mass concentration is 0.03%；
（2）Freezing：Immersion seed is placed in freeze drier, temperature is -30 DEG C, is freeze-dried 5 hours, reduces dormancy material
Active, seed is quickly received high temperature and low temperature stimulation, simulate the extraneous high/low temperature change being after planting subjected to, improve seed
Germinating capacity, accelerate germination, improve germination percentage, obtain freezing seed；
（3）Enzymolysis：Freezing seed is placed in enzymolysis liquid, digested 2 hours in 40 DEG C, destroys the institutional framework of kind of skin, is accelerated outer
Boundary's nutritional ingredient immerses seed, there is provided the nutriment needed for germination, accelerates plumule growth, filtering, obtains enzymolysis seed；Institute
The enzymolysis liquid stated, it is made up of the enzyme preparation of following parts by weight：Cellulase 16, hemicellulase 11, lignoenzyme 8, lipase
3rd, pectase 3, all enzyme preparations are mixed, it is 0.03% to add water to adjust to the mass concentration of enzymolysis liquid；
（4）Vernalization：Enzymolysis seed being placed in germinating box, 1 ~ 3 day, regulation temperature is 24 DEG C, humidity 76%, dark, 4 ~ 6 days,
Regulation temperature is 22 DEG C, humidity 81%, illumination 80lx, first hot and humid promotion plumule growth, then carry out dim light illumination, is dropped
Low temperature, make plumule gradually sturdy, strengthen resistance, make after planting fast germination, improve germination percentage, obtain vernalization seed；
（5）Sowing：Vernalization seed is conventionally sowed, obtains sowing seed.
Embodiment 2
A kind of pre-sowing treatment method of sealwort seed, comprises the following steps：
（1）Immersion：Sealwort seed that is ripe, complete, newly harvesting is selected, is placed in the copper-bath that mass concentration is 0.7%,
Soaked 4 hours in 43 DEG C, quickening kind soft and soggyization, kill the disease pest that retains in seed, promote sodium selenite and copper sulphate to penetrate into kind
Son, dormancy species activity in seed is reduced, accelerate germination, draining, obtain immersion seed；Described copper-bath, also contains
There is the sodium selenite that mass concentration is 0.04%；
（2）Freezing：Immersion seed is placed in freeze drier, temperature is -29 DEG C, is freeze-dried 6 hours, reduces dormancy material
Active, seed is quickly received high temperature and low temperature stimulation, simulate the extraneous high/low temperature change being after planting subjected to, improve seed
Germinating capacity, accelerate germination, improve germination percentage, obtain freezing seed；
（3）Enzymolysis：Freezing seed is placed in enzymolysis liquid, digested 2.5 hours in 41 DEG C, destroys the institutional framework of kind of skin, is accelerated
External nourishment composition immerses seed, there is provided the nutriment needed for germination, accelerates plumule growth, filtering, obtains enzymolysis seed；
Described enzymolysis liquid, it is made up of the enzyme preparation of following parts by weight：Cellulase 17, hemicellulase 12, lignoenzyme 9, fat
Enzyme 4, pectase 4, all enzyme preparations are mixed, and it is 0.04% to add water to adjust to the mass concentration of enzymolysis liquid；
（4）Vernalization：Enzymolysis seed being placed in germinating box, 1 ~ 3 day, regulation temperature is 24.5 DEG C, humidity 77%, dark, 4 ~ 6
My god, regulation temperature is 22.5 DEG C, humidity 82%, illumination 80lx, first hot and humid promotion plumule growth, then carry out dim light light
According to reducing temperature, make plumule gradually sturdy, strengthen resistance, make after planting fast germination, improve germination percentage, obtain vernalization seed；
（5）Sowing：Vernalization seed is conventionally sowed, obtains sowing seed.
Embodiment 3
A kind of pre-sowing treatment method of sealwort seed, comprises the following steps：
（1）Immersion：Sealwort seed that is ripe, complete, newly harvesting is selected, is placed in the copper-bath that mass concentration is 0.8%,
Soaked 5 hours in 45 DEG C, quickening kind soft and soggyization, kill the disease pest that retains in seed, promote sodium selenite and copper sulphate to penetrate into kind
Son, dormancy species activity in seed is reduced, accelerate germination, draining, obtain immersion seed；Described copper-bath, also contains
There is the sodium selenite that mass concentration is 0.05%；
（2）Freezing：Immersion seed is placed in freeze drier, temperature is -28 DEG C, is freeze-dried 7 hours, reduces dormancy material
Active, seed is quickly received high temperature and low temperature stimulation, simulate the extraneous high/low temperature change being after planting subjected to, improve seed
Germinating capacity, accelerate germination, improve germination percentage, obtain freezing seed；
（3）Enzymolysis：Freezing seed is placed in enzymolysis liquid, digested 3 hours in 42 DEG C, destroys the institutional framework of kind of skin, is accelerated outer
Boundary's nutritional ingredient immerses seed, there is provided the nutriment needed for germination, accelerates plumule growth, filtering, obtains enzymolysis seed；Institute
The enzymolysis liquid stated, it is made up of the enzyme preparation of following parts by weight：Cellulase 18, hemicellulase 13, lignoenzyme 10, lipase
5th, pectase 5, all enzyme preparations are mixed, it is 0.05% to add water to adjust to the mass concentration of enzymolysis liquid；
（4）Vernalization：Enzymolysis seed being placed in germinating box, 1 ~ 3 day, regulation temperature is 25 DEG C, humidity 78%, dark, 4 ~ 6 days,
Regulation temperature is 23 DEG C, humidity 83%, illumination 100lx, first hot and humid promotion plumule growth, then carry out dim light illumination, is dropped
Low temperature, make plumule gradually sturdy, strengthen resistance, make after planting fast germination, improve germination percentage, obtain vernalization seed；
（5）Sowing：Vernalization seed is conventionally sowed, obtains sowing seed.
Comparative example 1
Removal step（1）In copper-bath, remaining method, with embodiment 1.
Comparative example 2
Removal step（1）In sodium selenite, remaining method, with embodiment 1.
Comparative example 3
Step（1）In 40 DEG C of immersions be changed to soak at room temperature, remaining method, with embodiment 1.
Comparative example 4
Removal step（2）, remaining method, with embodiment 1.
Comparative example 5
Removal step（3）, remaining method, with embodiment 1.
Comparative example 6
Step（4）In germination temperature be always maintained at 15 DEG C, remaining method, with embodiment 1.
Comparative example 7
Step（4）In germination temperature illumination always be 80lx, remaining method, with embodiment 1.
Comparative example 8
The traditional treatment method of existing sealwort seed.
The treatment effect of embodiment and comparative example sealwort method for treating seeds：
In Anhui Province, 11000, the sealwort seed of mature and plump is chosen, 11 groups is randomly divided into, every group 1000, uses respectively real
The processing of the method progress sealwort seed of example and comparative example is applied, is conventionally sowed after processing, other management methods
Identical, record each group sealwort seed starts germinate unearthed time and germination percentage, embodiment and comparative example sealwort seed treatment side
The treatment effect of method is shown in Table 1.
Table 1：The treatment effect of embodiment and comparative example sealwort method for treating seeds
| Project | Germinate unearthed time/(d) | Germination percentage/(%) |
| Embodiment 1 | 18 | 95.1 |
| Embodiment 2 | 17 | 95.4 |
| Embodiment 3 | 19 | 94.8 |
| Comparative example 1 | 37 | 90.3 |
| Comparative example 2 | 44 | 89.7 |
| Comparative example 3 | 33 | 91.2 |
| Comparative example 4 | 58 | 84.6 |
| Comparative example 5 | 49 | 86.3 |
| Comparative example 6 | 43 | 88.6 |
| Comparative example 7 | 28 | 92.2 |
| Comparative example 8 | 827 | 64.1 |
Show from the result of table 1, the pre-sowing treatment method of the sealwort seed of embodiment, germination unearthed time is substantially compared with comparative example
Short, germination percentage is substantially high compared with comparative example, illustrates the pre-sowing treatment method of sealwort seed provided by the invention and has processing well
Effect.
Claims (4)
- A kind of 1. pre-sowing treatment method of sealwort seed, it is characterised in that comprise the following steps：（1）Immersion：Sealwort seed that is ripe, complete, newly harvesting is selected, is placed in the copper-bath that mass concentration is 0.6 ~ 0.8% In, soaked 3 ~ 5 hours in 40 ~ 45 DEG C, draining, obtain immersion seed；（2）Freezing：Immersion seed is placed in freeze drier, is freeze-dried 5 ~ 7 hours, obtains freezing seed；（3）Enzymolysis：Freezing seed is placed in enzymolysis liquid, digested 2 ~ 3 hours in 40 ~ 42 DEG C, filtering, obtains enzymolysis seed；（4）Vernalization：Enzymolysis seed being placed in germinating box, 1 ~ 3 day, regulation temperature is 24 ~ 25 DEG C, humidity is 76 ~ 78%, dark, 4 ~ 6 days, regulation temperature was 22 ~ 23 DEG C, humidity is that 81 ~ 83%, illumination is 80 ~ 100lx, obtains vernalization seed；（5）Sowing：Vernalization seed is conventionally sowed, obtains sowing seed.
- 2. the pre-sowing treatment method of sealwort seed according to claim 1, it is characterised in that the step（1）Copper sulphate Solution, also containing the sodium selenite that mass concentration is 0.03 ~ 0.05%.
- 3. the pre-sowing treatment method of sealwort seed according to claim 1, it is characterised in that the step（2）Freezing do Dry, temperature is -30 ~ -28 DEG C.
- 4. the pre-sowing treatment method of sealwort seed according to claim 1, it is characterised in that the step（3）Enzymolysis liquid, It is made up of the enzyme preparation of following parts by weight：Cellulase 16 ~ 18, hemicellulase 11 ~ 13, lignoenzyme 8 ~ 10, lipase 3 ~ 5th, pectase 3 ~ 5, all enzyme preparations are mixed, it is 0.03 ~ 0.05% to add water to adjust to the mass concentration of enzymolysis liquid.
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| CN201710878645.9A CN107484462A (en) | 2017-09-26 | 2017-09-26 | A kind of pre-sowing treatment method of sealwort seed |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511313A (en) * | 2019-01-15 | 2019-03-26 | 安徽省科学技术研究院 | A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method |
| CN112335371A (en) * | 2020-12-09 | 2021-02-09 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of polygonatum sibiricum seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
-
2017
- 2017-09-26 CN CN201710878645.9A patent/CN107484462A/en not_active Withdrawn
Non-Patent Citations (2)
| Title |
|---|
| 梁建萍: "《植物学》", 28 February 2014, 中国农业出版社 * |
| 段碧华，李琳，冯彩云: "《林下特色农业实用技术》", 31 March 2013, 中国农业科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511313A (en) * | 2019-01-15 | 2019-03-26 | 安徽省科学技术研究院 | A kind of presprouting of seeds agent of polygonatum cyrtonema and its germination accelerating method |
| CN112335371A (en) * | 2020-12-09 | 2021-02-09 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of polygonatum sibiricum seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
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