CN101642065A - Attachment base processing method for improving abnormal attachment of stichoposide seeds - Google Patents
Attachment base processing method for improving abnormal attachment of stichoposide seeds Download PDFInfo
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- CN101642065A CN101642065A CN200910015995A CN200910015995A CN101642065A CN 101642065 A CN101642065 A CN 101642065A CN 200910015995 A CN200910015995 A CN 200910015995A CN 200910015995 A CN200910015995 A CN 200910015995A CN 101642065 A CN101642065 A CN 101642065A
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- China
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- adherance
- corrugated plating
- processing method
- attachment base
- diatom
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to an attachment base processing method for improving abnormal attachment of stichoposide seeds, which is characterized by comprising the following steps: selecting a polyethylene corrugated plate as an attachment base; then, processing the attachment base by a biological film containing diatom, or soaking the attachment base in the adult tissue extracting solution; and finally, cultivating stichoposide seeds. Based on the feasibility of actual seed production of stichoposide, the invention can obviously improve the abnormal attachment rate of pelagic larvae and the rateof emergence by selecting the proper attachment base and simply processing the attachment base under the condition that the production cost and the manual work are not increased greatly.
Description
Technical field
The present invention relates to a kind of adherance processing method that juvenile stichopus metamorphosis is adhered to that improves.
Background technology
Stichopus japonicus is the hat of " marine products eight delicacies ", and natural distribution is the important marine economic animal of China in the East Asia Region of China, Japan and Korea S.Over year, along with the sustainable growth of the exhausted and domestic day by day sea cucumber consumption of global sea cucumber natural resources, China's apostichopus japonicus culture presents fast development situation surplus in the of nearly 10.According to statistics, be higher than 5.6% of the national aquatic products output average annual growth same period to the average annual growth by 12.2% of 2006 annual productions in 2003.Wherein, the sea cucumber gross yield was about 7.57 ten thousand tons in 2006, and cultured area reaches 8.42 ten thousand hectares.At present, apostichopus japonicus culture has become aquaculture industry with local characteristic and new marine economy growth point on Shandong, Liaoning and other places.
The development of apostichopus japonicus culture industry has benefited from the breakthrough and the maturation of artificial breeding technique, and the ginseng kind of cultivating large area mainly obtains by artificial breeding.The fertilized egg of stichopus japonicus through blastula stage, gastrul stage, auricularia larvas after, individually be attached to the end life of dwelling by the original life metamorphosis of swimming.Adhere to period because young ecological change is bigger in metamorphosis, tend to cause young mortality, become the bottleneck that the restriction juvenile stichopus is produced.The selection of suitable adherance can improve the abnormal adhesive rate of seed in the seedling raising process effectively, improves seed production efficiency.Carried out number of research projects in seashells, the metamorphosis that discovery has many physics, chemistry and biological factor to influence the shellfish larva is adhered to.The application of these results of study aspect aquaculture can effectively be shortened the young and adhere to and the abnormal time, reduces lethality, increases the output of seedling in the seed production.But the research of relevant stichopus japonicus larva settlement and metamorphosis inducement rarely has report.
Summary of the invention
The purpose of this invention is to provide a kind of adherance processing method that juvenile stichopus metamorphosis is adhered to that improves, improve the abnormal deposit efficiency of seed effectively, thereby improve the production efficiency of seed, create more economic benefit by processing to adherance.
A kind ofly improve the adherance processing method that juvenile stichopus metamorphosis is adhered to, it is characterized in that selecting the polyethylene corrugated plating to make adherance, handle or adult tissue's extract immersion treatment with the biomembrane that is rich in diatom then, carry out juvenile stichopus at last and cultivate.
The present invention is based on the feasibility that the actual seed of stichopus japonicus produces, and increases under production cost and the artificial situation exceeding, and by choosing suitable adherance and adherance simply being handled, can obviously improve the abnormal adhesive rate of floating larvae, improves output of seedling.
Embodiment
1, select the polyethylene corrugated plating as adherance
When larvae development during to big ear larva latter stage or doliolarias, throw in respectively the polyethylene corrugated plating (long * wide: 100 * 150mm), screen cloth (long * wide: 200 * 300mm) and polyethylene film (length * wide: 40 * 300mm) as adherance.Before using above-mentioned three kinds of adherances are used sea water immersion respectively, use concentration (5-10) * 10 again
-4Liquor potassic permanganate soak, clean with clear water then, dry.After 5 days, add up the larva number that adheres on each adherance, calculate the abnormal adhesive rate of larva.
The result shows that in above-mentioned 3 kinds of different adherances, the abnormal adhesive rate of polyethylene corrugated plating is up to 29.3%, is polyethylene film 18.4% secondly, and minimum is screen cloth 14.7%.Use the polyethylene corrugated plating as adherance, the abnormal adhesive rate of larva is significantly higher than other two kinds of adherances (P＜0.05).
2, the biomembrane of adherance is handled
Polyethylene corrugated plating adherance is carried out 4 kinds of simple preliminary treatment respectively: 1) the polyethylene corrugated plating is cleaned and dried, in contrast; 2) the polyethylene corrugated plating of cleaning was soaked in nature seawater 4-6 days, make its surface form the common bacterial biof iotalm of one deck; 3) the polyethylene corrugated plating of cleaning was soaked 4-6 days in the seawater of inflation cultivation diatom (little Nitzschia closterium minutissima), make its surface form one deck and be rich in the biomembrane of diatom; 4) the polyethylene corrugated plating of cleaning was soaked 4-6 days in the seawater of inflation cultivation probio (EM), make its surface form one deck and be rich in the biomembrane of probio.
The result shows that with the adherance of above-mentioned 4 kinds of different disposal, the larval metamorphosis adhesive rate is followed successively by from high to low: the biomembrane that is rich in diatom is 53.5%, be rich in the biomembrane 50.7% of probio, common bacterial biof iotalm 40.2% and inanimate object film 32.4%.The abnormal adhesive rate of handling through biomembrane of adherance is significantly higher than undressed adherance (P＜0.05).The abnormal adhesive rate of biomembrane that is rich in diatom is not obvious with the abnormal adhesive rate difference of the biomembrane that is rich in probio, but both are significantly higher than common bacterial biof iotalm (P＜0.05).
3, adult tissue's extract immersion treatment of adherance
The polyethylene corrugated plating of cleaning is immersed in adult tissue's extract 1-2 days, dries use then.When preparing this adult tissue extract, the chopping of adult stichopus japonicus tissue is ground, add the 500-1000mL clear water and boiled 10-20 minute.The result shows that the polyethylene corrugated plating that uses adult tissue's extract immersion treatment is as adherance, and the abnormal adhesive rate of stichopus japonicus larva is 44.1%, apparently higher than undressed polyethylene corrugated plating (33.8%).
4, carry out the seed rearing of stichopus japonicus routinely
Adopt earlier dry in the shade, the seawater of intensification and ultraviolet irradiation carries out artificial induced spawning to the seed ginseng individuality, larva through come-up, select excellent after at 10m
3Cement pit in inflation cultivate, larval density is 100-150/liter, the water temperature of larvae cultivation is 19-21 ℃, salinity is 30-32 ‰, little inflation is cultivated.Throw something and feed in the cultivation little Nitzschia closterium minutissima (Nitzschia closterium), salt algae and Chaetoceros muelleri (Chaetoceros muelleri), one day 2-3 time; Change water 1-2 every day, changes 50% at every turn.After most of larval metamorphosis adheres to, begin the mixing bait of throwing something and feeding and disposing with dried mud and algae powder.
Above result shows that the polyethylene corrugated plating handling through the biomembrane that is rich in diatom or after adult tissue's extract soaks, can significantly improving the abnormal adhesive rate of stichopus japonicus larva, improves the emergence rate of nursery as a kind of suitable adherance.Adherance processing method of the present invention is easy, has stronger operability, is convenient to realize commercial application.
Claims (4)
1, a kind ofly improves the adherance processing method that juvenile stichopus metamorphosis is adhered to, it is characterized in that selecting the polyethylene corrugated plating to make adherance, handle or adult tissue's extract immersion treatment with the biomembrane that is rich in diatom then, carry out juvenile stichopus at last and cultivate.
2, according to the described adherance processing method of claim 1, it is characterized in that using sea water immersion before described polyethylene corrugated plating uses, use concentration (5-10) * 10 again
-4Liquor potassic permanganate soak, clean with clear water then, dry; When extremely big ear larva latter stage of stichopus japonicus larvae development or doliolarias, throw in corrugated plating, horizontal positioned.
3, according to the described adherance processing method of claim 1, it is characterized in that biomembrane that described usefulness is rich in diatom is handled is meant the polyethylene corrugated plating was soaked 4-6 days in the seawater of cultivating diatom, forms one deck and be rich in the biomembrane of diatom on the corrugated plating surface.
4, according to the described adherance processing method of claim 1, it is characterized in that described adult tissue extract immersion treatment is meant one-tenth ginseng tissue chopping grinding, add the 500-1000mL clear water and boiled 10-20 minute, get supernatant and soaked corrugated plating 1-2 days, dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910015995A CN101642065A (en) | 2009-06-10 | 2009-06-10 | Attachment base processing method for improving abnormal attachment of stichoposide seeds |
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|---|---|---|---|
| CN200910015995A CN101642065A (en) | 2009-06-10 | 2009-06-10 | Attachment base processing method for improving abnormal attachment of stichoposide seeds |
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| CN101642065A true CN101642065A (en) | 2010-02-10 |
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| CN200910015995A Pending CN101642065A (en) | 2009-06-10 | 2009-06-10 | Attachment base processing method for improving abnormal attachment of stichoposide seeds |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940173A (en) * | 2010-07-22 | 2011-01-12 | 中国海洋大学 | Method for acquiring benthonic microbe bred stichopus japonicus by using corrugated plate |
| CN102067822A (en) * | 2010-10-25 | 2011-05-25 | 山东东方海洋科技股份有限公司 | Method for stripping sea cucumber from attaching base |
| CN102524127A (en) * | 2012-01-11 | 2012-07-04 | 中国海洋大学 | Sea cucumber seed settlement substrate with settling bacterial membranes and method of producing sea cucumber seed settlement substrate with settling bacterial membranes |
| CN102870723A (en) * | 2012-11-05 | 2013-01-16 | 扬州大学 | Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum |
| CN103270986A (en) * | 2013-06-03 | 2013-09-04 | 山东省海洋水产研究所 | Application of KCl in artificial induction of attachment and metamorphosis in larval Apostichopus japonicus |
| CN104531532A (en) * | 2014-12-12 | 2015-04-22 | 中国海洋大学 | Luticola sp as well as application and cultivation method thereof |
| CN109769723A (en) * | 2018-12-26 | 2019-05-21 | 广西壮族自治区水产科学研究院 | A method of using bottom dwell food organisms film cultivation river snail |
-
2009
- 2009-06-10 CN CN200910015995A patent/CN101642065A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940173A (en) * | 2010-07-22 | 2011-01-12 | 中国海洋大学 | Method for acquiring benthonic microbe bred stichopus japonicus by using corrugated plate |
| CN102067822A (en) * | 2010-10-25 | 2011-05-25 | 山东东方海洋科技股份有限公司 | Method for stripping sea cucumber from attaching base |
| CN102524127A (en) * | 2012-01-11 | 2012-07-04 | 中国海洋大学 | Sea cucumber seed settlement substrate with settling bacterial membranes and method of producing sea cucumber seed settlement substrate with settling bacterial membranes |
| CN102870723A (en) * | 2012-11-05 | 2013-01-16 | 扬州大学 | Sea cucumber larvae attachment substratum adhered with benthic diatom membrane with high induction activity and preparation method of sea cucumber larvae attachment substratum |
| CN103270986A (en) * | 2013-06-03 | 2013-09-04 | 山东省海洋水产研究所 | Application of KCl in artificial induction of attachment and metamorphosis in larval Apostichopus japonicus |
| CN103270986B (en) * | 2013-06-03 | 2014-09-03 | 山东省海洋水产研究所 | Application of KCL in artificial induction of attachment and metamorphosis in larval Apostichopus japonicus |
| CN104531532A (en) * | 2014-12-12 | 2015-04-22 | 中国海洋大学 | Luticola sp as well as application and cultivation method thereof |
| CN104531532B (en) * | 2014-12-12 | 2017-05-10 | 中国海洋大学 | Luticola sp as well as application and cultivation method thereof |
| CN109769723A (en) * | 2018-12-26 | 2019-05-21 | 广西壮族自治区水产科学研究院 | A method of using bottom dwell food organisms film cultivation river snail |
| CN109769723B (en) * | 2018-12-26 | 2021-10-15 | 广西壮族自治区水产科学研究院 | Method for breeding river snails by adopting benthic bait biomembrane |
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Open date: 20100210 |