CN102630612A - Cultivation method of Patinopecten yessoensis Jay with red-white flower pattern - Google Patents
Cultivation method of Patinopecten yessoensis Jay with red-white flower pattern Download PDFInfo
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- CN102630612A CN102630612A CN2012101166950A CN201210116695A CN102630612A CN 102630612 A CN102630612 A CN 102630612A CN 2012101166950 A CN2012101166950 A CN 2012101166950A CN 201210116695 A CN201210116695 A CN 201210116695A CN 102630612 A CN102630612 A CN 102630612A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a cultivation method using double-white-shell Patinopecten yessoensis Jay and ordinary purple-brown-shell Patinopecten yessoensis Jay as parent pectinid through single cross and group cross as well as the corresponding breeding methods to obtain the Patinopecten yessoensis Jay with a red-white flower pattern on the shell, the red-white-flower Patinopecten yessoensis Jay can be used for studying shellfish shell colors and is used as an ideal experimental material for research of correlated characters; and meanwhile, the red-white-flower Patinopecten yessoensis Jay is used as a base material for variety breeding of the Patinopecten yessoensis Jay. The operation method provided by the invention is simple without the introduction of foreign genes by means of complex technologies such as transgenosis and has no influence on the security of living beings and environment.
Description
Technical field
The present invention relates to a kind of breeding method of Patinopecten yessoensis, especially a kind of can be as the red breeding method of spending Patinopecten yessoensis in vain of shell look and proterties correlation research and improvement experiment kind.
Background technology
Patinopecten yessoensis (
Patinopecten yessoensisJay) belong to Mollusca (Mollusca), lamellibranchiata (Lamellibranchia), Pectenidae (Pectinidae) shellfish, belong to cold water property marine shellfish.Usually Patinopecten yessoensis is a puce shell Patinopecten yessoensis, and its shell is fan-shaped, and right shell is more outstanding, is yellow-white or milky, below being positioned at during life; Left side shell is flat slightly, and is slightly littler than right shell, is puce, is upper casing.Because the design and color of shell obtains through evolving, color, the complicated shellfish of pattern possess good and economic shape and identifiability, can be as the correlation research and the improvement experiment kind of shell look and proterties.Though Chinese patent number is 200710012477.1 patent of invention; A kind of " cultural method of double white shell Japanese scallop " disclosed; Can cultivate double white shell Japanese scallop, but the shell look still is monochromatic (white), can not demonstrate fully above-mentioned advantage with industrial value.
Summary of the invention
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, to provide a kind of and can and improve the red breeding method of spending Patinopecten yessoensis in vain of testing kind as shell look and proterties correlation research.
Technical solution of the present invention is: a kind of red breeding method of spending Patinopecten yessoensis in vain is characterized in that carrying out according to following steps:
A. double white shell Japanese scallop and puce shell Patinopecten yessoensis individuality are 16 ~ 18/m as close shellfish with density completely to choose gonad development
3Carry out temporarily supporting, full dose is changed water 1 time every day during supporting temporarily; The said condition of supporting temporarily is: in 1 ~ 3 ℃ of water temperature, store up and stablize 3 days; Day intensification is 0.3～0.4 ℃ then, stablizes when water temperature is raised to 4～5 ℃ 4 days, and day intensification is 0.3～0.5 ℃ again; Constant temperature was cultivated to gonad maturity when water temperature was raised to 6 ~ 7 ℃; Double white shell Japanese scallop and puce shell Patinopecten yessoensis are carried out hero, dam shellfish divide pool cultivatedly, 6 ~ 7 ℃ of temperature of cultivation continue to cultivate 10～12 days;
B. after close shellfish discharging, continue the constant temperature breed and carried out artificial induced spawning in 3～4 days, when treating that close shellfish is discharged in a large number, collect sperm and ovum respectively;
C. get double white shell Japanese scallop sperm and puce Patinopecten yessoensis ovum and double white shell Japanese scallop ovum respectively and puce Patinopecten yessoensis sperm carries out artificial insemination one to one, the sperm consumption is that 2～3 sperms are arranged around the ovum;
D. fertilized egg is hatched, 40～50/ml of hatching density, 11～12 ℃ of water temperatures of hatching, every interval 1h stirs the pond once, hatch to development of fertilized ova be D type larva;
E. after D type larva all floats, go out to cultivate with 260 mesh sieve bolting silks, density 8～9/ml in cloth pond cultivates 11～12 ℃ of pond water temperatures, throws open-mouthed bait 8000-10000cell/ml chrysophyceae, day bait throwing in 3～4 times; Change water every day 2 times, change water 1/2 ~ 2/3 at every turn, every at a distance from 4 ~ 5 days the pond once cultivate to D type larva and eyespot occurs;
F. the eyespot larva is changed the pond and cultivate, cultivate 11～12 ℃ of pond water temperatures, cultivating density is 6～7/ml, and cultivating temperature is to heat up 0.4 ~ 0.6 ℃ every day, rise to 16 ℃ after constant temperature culture, other breeding condition is with the e step;
G. when 35 ~ 40% eyespot larva is abnormal juvenile mollusk, throws in adherance and supply abnormal juvenile mollusk to adhere to, during every day change water 2 times, change water more than 70% at every turn, increase the chrysophyceae daily ration, feeding quantity to 50000cell/ml, day change water 2～3 times, each 1/2 ~ 2/3;
The sea is hung in outbound when h. continuing to cultivate to high 600 ~ 640 μ m of shell, breeds in the middle of being hung on buoyant raft with the net bagged seedlings, outbound is preceding indoor water temperature is reduced to gradually consistent with ocean temperature; When juvenile mollusk shell during, assign in the 30 order mesh bags up to 0.2～0.3cm;
I. when cultivating juvenile mollusk shell height and be 0.5 ~ 0.7cm, the juvenile mollusk of same mesh bag is assigned in the different mesh bags or juvenile mollusk put into cylinder mould is cultured or bottom culture is broadcast at the end;
J. when cultivating to gonadal maturation, choose each 20 close shellfish of conduct of female, the male Patinopecten yessoensis of puce therein, carry out supporting temporarily according to a step, the b step is hastened parturition;
K. collect sperm and the ovum that the j step produced and inseminate, the sperm consumption is that 2～3 sperms are arranged around the ovum;
Repeat the step of d～i and breed cultivation, bred for 1 ~ 2 generation, promptly obtain into magenta and spend Patinopecten yessoensis in vain.
The present invention is to be close shellfish with double white shell Japanese scallop and common puce, and through single cross and flock-mate and corresponding breeding method, promptly obtaining the shell mask has the red Patinopecten yessoensis of spending pattern in vain.It is red that to spend the pattern Patinopecten yessoensis in vain all significant aspect scientific research and production.At first, can utilize it to carry out the mechanism research of shellfish shell look, pattern; Secondly, can spend Patinopecten yessoensis research shell look, pattern and economic characters correlation in vain through red, and then select the Patinopecten yessoensis new varieties (being) of actual application value; In addition, the red pattern of spending in vain can be used as the morphological markers of distinguishing different Patinopecten yessoensis colony, and to spend Patinopecten yessoensis shell colored pattern in vain rich and varied because of red, has reserve value.Method of operating of the present invention is simple, need not import foreign gene through complex technologys such as transgenosiss, and biological and Environmental security are had no influence.
Embodiment
A. double white shell Japanese scallop and puce shell Patinopecten yessoensis individuality are 16 ~ 18/m as close shellfish with density completely to choose gonad development
3Carry out temporarily supporting, full dose is changed water 1 time every day during supporting temporarily; The said condition of supporting temporarily is: in 1 ~ 3 ℃ of water temperature, store up and stablize 3 days; Day intensification is 0.3～0.4 ℃ then, stablizes when water temperature is raised to 4～5 ℃ 4 days, and day intensification is 0.3～0.5 ℃ again; Constant temperature was cultivated to gonad maturity when water temperature was raised to 6 ~ 7 ℃; Double white shell Japanese scallop and puce shell Patinopecten yessoensis are carried out hero, dam shellfish divide pool cultivatedly, 6 ~ 7 ℃ of temperature of cultivation continue to cultivate 10～12 days;
B. after close shellfish discharging, continue the constant temperature breed and carried out artificial induced spawning in 3～4 days, when treating that close shellfish is discharged in a large number, collect sperm and ovum respectively;
C. get double white shell Japanese scallop sperm and puce Patinopecten yessoensis ovum and double white shell Japanese scallop ovum respectively and puce Patinopecten yessoensis sperm carries out artificial insemination one to one, the sperm consumption is that 2～3 sperms are arranged around the ovum;
D. fertilized egg is hatched, 40～50/ml of hatching density, 11～12 ℃ of water temperatures of hatching, every interval 1h stirs the pond once, hatch to development of fertilized ova be D type larva;
E. after D type larva all floats, go out to cultivate with 260 mesh sieve bolting silks, density 8～9/ml in cloth pond cultivates 11～12 ℃ of pond water temperatures, throws open-mouthed bait 8000-10000cell/ml chrysophyceae, day bait throwing in 3～4 times; Change water every day 2 times, change water 1/2 ~ 2/3 at every turn, every at a distance from 4 ~ 5 days the pond once cultivate to D type larva and eyespot occurs;
F. the eyespot larva is changed the pond and cultivate, cultivate 11～12 ℃ of pond water temperatures, cultivating density is 6～7/ml, and cultivating temperature is to heat up 0.4 ~ 0.6 ℃ every day, rise to 16 ℃ after constant temperature culture, other breeding condition is with the e step;
G. when 35 ~ 40% eyespot larva is abnormal juvenile mollusk, throws in adherance and supply abnormal juvenile mollusk to adhere to, during every day change water 2 times, change water more than 70% at every turn, increase the chrysophyceae daily ration, feeding quantity to 50000cell/ml, day change water 2～3 times, each 1/2 ~ 2/3;
The sea is hung in outbound when h. continuing to cultivate to high 600 ~ 640 μ m of shell, breeds in the middle of being hung on buoyant raft with the net bagged seedlings, outbound is preceding indoor water temperature is reduced to gradually consistent with ocean temperature; When juvenile mollusk shell during, assign in the 30 order mesh bags up to 0.2～0.3cm;
I. when cultivating juvenile mollusk shell height and be 0.5 ~ 0.7cm, the juvenile mollusk of same mesh bag is assigned in the different mesh bags or juvenile mollusk put into cylinder mould is cultured or bottom culture is broadcast at the end;
J. when cultivating to gonadal maturation, choose each 20 close shellfish of conduct of female, the male Patinopecten yessoensis of puce therein, carry out supporting temporarily according to a step, the b step is hastened parturition;
K. collect sperm and the ovum that the j step produced and inseminate, the sperm consumption is that 2～3 sperms are arranged around the ovum;
Repeat the step of d～i and breed cultivation, bred for 1 ~ 2 generation, promptly obtain a certain proportion of one-tenth magenta and spend Patinopecten yessoensis in vain.
Claims (1)
1. red breeding method of spending Patinopecten yessoensis in vain is characterized in that carrying out according to following steps:
A. double white shell Japanese scallop and puce shell Patinopecten yessoensis individuality are 16 ~ 18/m as close shellfish with density completely to choose gonad development
3Carry out temporarily supporting, full dose is changed water 1 time every day during supporting temporarily; The said condition of supporting temporarily is: in 1 ~ 3 ℃ of water temperature, store up and stablize 3 days; Day intensification is 0.3～0.4 ℃ then, stablizes when water temperature is raised to 4～5 ℃ 4 days, and day intensification is 0.3～0.5 ℃ again; Constant temperature was cultivated to gonad maturity when water temperature was raised to 6 ~ 7 ℃; Double white shell Japanese scallop and puce shell Patinopecten yessoensis are carried out hero, dam shellfish divide pool cultivatedly, 6 ~ 7 ℃ of temperature of cultivation continue to cultivate 10～12 days;
B. after close shellfish discharging, continue the constant temperature breed and carried out artificial induced spawning in 3～4 days, when treating that close shellfish is discharged in a large number, collect sperm and ovum respectively;
C. get double white shell Japanese scallop sperm and puce Patinopecten yessoensis ovum and double white shell Japanese scallop ovum respectively and puce Patinopecten yessoensis sperm carries out artificial insemination one to one, the sperm consumption is that 2～3 sperms are arranged around the ovum;
D. fertilized egg is hatched, 40～50/ml of hatching density, 11～12 ℃ of water temperatures of hatching, every interval 1h stirs the pond once, hatch to development of fertilized ova be D type larva;
E. after D type larva all floats, go out to cultivate with 260 mesh sieve bolting silks, density 8～9/ml in cloth pond cultivates 11～12 ℃ of pond water temperatures, throws open-mouthed bait 8000-10000cell/ml chrysophyceae, day bait throwing in 3～4 times; Change water every day 2 times, change water 1/2 ~ 2/3 at every turn, every at a distance from 4 ~ 5 days the pond once cultivate to D type larva and eyespot occurs;
F. the eyespot larva is changed the pond and cultivate, cultivate 11～12 ℃ of pond water temperatures, cultivating density is 6～7/ml, and cultivating temperature is to heat up 0.4 ~ 0.6 ℃ every day, rise to 16 ℃ after constant temperature culture, other breeding condition is with the e step;
G. when 35 ~ 40% eyespot larva is abnormal juvenile mollusk, throws in adherance and supply abnormal juvenile mollusk to adhere to, during every day change water 2 times, change water more than 70% at every turn, increase the chrysophyceae daily ration, feeding quantity to 50000cell/ml, day change water 2～3 times, each 1/2 ~ 2/3;
The sea is hung in outbound when h. continuing to cultivate to high 600 ~ 640 μ m of shell, breeds in the middle of being hung on buoyant raft with the net bagged seedlings, outbound is preceding indoor water temperature is reduced to gradually consistent with ocean temperature; When juvenile mollusk shell during, assign in the 30 order mesh bags up to 0.2～0.3cm;
I. when cultivating juvenile mollusk shell height and be 0.5 ~ 0.7cm, the juvenile mollusk of same mesh bag is assigned in the different mesh bags or juvenile mollusk put into cylinder mould is cultured or bottom culture is broadcast at the end;
J. when cultivating to gonadal maturation, choose each 20 close shellfish of conduct of female, the male Patinopecten yessoensis of puce therein, carry out supporting temporarily according to a step, the b step is hastened parturition;
K. collect sperm and the ovum that the j step produced and inseminate, the sperm consumption is that 2～3 sperms are arranged around the ovum;
Repeat the step of d～i and breed cultivation, bred for 1 ~ 2 generation, promptly obtain into magenta and spend Patinopecten yessoensis in vain.
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| CN2012101166950A CN102630612A (en) | 2012-04-20 | 2012-04-20 | Cultivation method of Patinopecten yessoensis Jay with red-white flower pattern |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168734A (en) * | 2013-04-15 | 2013-06-26 | 蓬莱市天保海水养殖有限公司 | Parent patinopecten yessoensis culturing and hatching method |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN104885978A (en) * | 2015-06-02 | 2015-09-09 | 大连海洋大学 | Method for breeding by aid of one-year-old patinopecten yesoensis |
| CN107148928A (en) * | 2017-05-19 | 2017-09-12 | 中国海洋大学 | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids |
| CN108849658A (en) * | 2018-06-11 | 2018-11-23 | 广东海洋大学 | A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate |
| CN109287533A (en) * | 2018-09-17 | 2019-02-01 | 大连海洋大学 | Scallop hybrid seed method for scaled breeding and equipment |
| CN110419473A (en) * | 2019-09-18 | 2019-11-08 | 大连明成水产食品有限公司 | A kind of Patinopecten yessoensis method for culturing seedlings |
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| CN102308770A (en) * | 2011-08-05 | 2012-01-11 | 青岛农业大学 | Breeding method for new marine backcrossed scallop variety |
| CN102318571A (en) * | 2011-08-05 | 2012-01-18 | 青岛农业大学 | The breeding method of the purple scallop hybrid new varieties in sea |
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| JP2001161206A (en) * | 1999-12-13 | 2001-06-19 | Kerama Shinju:Kk | Method for producing pinctada fucata martensii of japan growth |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168734A (en) * | 2013-04-15 | 2013-06-26 | 蓬莱市天保海水养殖有限公司 | Parent patinopecten yessoensis culturing and hatching method |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN103416336B (en) * | 2013-08-16 | 2014-09-17 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN104885978A (en) * | 2015-06-02 | 2015-09-09 | 大连海洋大学 | Method for breeding by aid of one-year-old patinopecten yesoensis |
| CN104885978B (en) * | 2015-06-02 | 2017-04-19 | 大连海洋大学 | Method for breeding by aid of one-year-old patinopecten yesoensis |
| CN107148928A (en) * | 2017-05-19 | 2017-09-12 | 中国海洋大学 | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids |
| CN107148928B (en) * | 2017-05-19 | 2020-07-24 | 中国海洋大学 | Method for cultivating hybrid variety of patinopecten yessoensis and wind vane scallop |
| CN108849658A (en) * | 2018-06-11 | 2018-11-23 | 广东海洋大学 | A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate |
| CN109287533A (en) * | 2018-09-17 | 2019-02-01 | 大连海洋大学 | Scallop hybrid seed method for scaled breeding and equipment |
| CN109287533B (en) * | 2018-09-17 | 2024-02-27 | 大连海洋大学 | Large-scale breeding method and equipment for hybrid scallop offspring seeds |
| CN110419473A (en) * | 2019-09-18 | 2019-11-08 | 大连明成水产食品有限公司 | A kind of Patinopecten yessoensis method for culturing seedlings |
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Application publication date: 20120815 |