CN104885978A - Method for breeding by aid of one-year-old patinopecten yesoensis - Google Patents

Method for breeding by aid of one-year-old patinopecten yesoensis Download PDF

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CN104885978A
CN104885978A CN201510294317.5A CN201510294317A CN104885978A CN 104885978 A CN104885978 A CN 104885978A CN 201510294317 A CN201510294317 A CN 201510294317A CN 104885978 A CN104885978 A CN 104885978A
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shellfish
day
breeding
feed
close shellfish
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CN104885978B (en
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常亚青
庞云龙
丁君
宋坚
张伟杰
田莹
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Dalian Ocean University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a method for breeding by the aid of one-year-old patinopecten yesoensis. The method has the advantages that the one-year-old patinopecten yesoensis can be propagated, accordingly, the variety production age limit of the patinopecten yesoensis can be directly reduced, the generation reproduction can be accelerated, the variety breeding speeds can be increased, the sexual maturity time of the patinopecten yesoensis can be shortened and is one year instead of the original two years owing to operation such as parent patinopecten yesoensis indoor enhanced cultivation, parent patinopecten yesoensis maturity promotion and breeding, and technical support can be provided for searching for time-saving, labor-saving and cost-reducing processes for breeding seeds by the aid of patinopecten yesoensis.

Description

Utilize one age Patinopecten yessoensis carry out the method for breeding
Technical field
The present invention relates to a kind of breeding method of Patinopecten yessoensis, particularly a kind of utilize one age Patinopecten yessoensis carry out the method for breeding.
Background technology
Patinopecten yessoensis (Mizuhopecten yessoensis Jay)belong to Mollusca (Mollusa), lamellibranchiata (Lamellibranchia), wing subclass (Pterimorphia), pearl shell order (Pterioida), Pectenidae (Pectinidae).After introducing China from Japan the eighties, the ground such as Liaoning, Shandong is promoted again, and cultured area constantly expands, for China's mariculture industry brings huge economic benefit.Under natural conditions, Patinopecten yessoensis 2 reaches sexual maturity age, can carry out nature or artificial propagation afterwards.For animals and plants breeding, shorten breeding time, improve breeding efficiency most important, such as, crops " Nan Fan ", be exactly the weather conditions utilizing south warm, in a north generation in crop breeding material summer, move to south winter and plant a generation or two generations again, the alternately plantation of such south, the north, just can accelerate propagation generations, accelerate breed of variety speed.If the sexual maturity time of Patinopecten yessoensis therefore can be accelerated, just great economic interests can be brought for the cultivation of Patinopecten yessoensis.But also there is no a kind of method that can achieve the above object now.
Summary of the invention
The present invention is the above-mentioned deficiency in order to solve existing for prior art, propose one can allow one age Patinopecten yessoensis reach sexual maturity, and the breeding method of artificial propagation can be carried out.
Technical solution of the present invention is: a kind of utilize one age Patinopecten yessoensis carry out the method for breeding, it is characterized in that: described method is carried out according to following steps:
In October, the average temperature as continuous 7 days maritime interior waters is less than and equals 20 DEG C, from sea area hanging culture Patinopecten yessoensis in select the Patinopecten yessoensis of the long 27 ± 1mm of shell as close shellfish, carry out hanging between indoor cart growing, every layer of cage placed 6-8 only close shellfish,
The cultivation stage of parent shellfish is divided into early stage, mid-term and later stage, and the cultivation water temperature in early stage is 15-20 DEG C, and the time is 22d, and the cultivation water temperature in mid-term is 10-15 DEG C, time is 22d, and the cultivation water temperature in later stage is 4-10 DEG C, and the time is 26d, on average lower the temperature in the training period, lower the temperature every day 0.2-0.5 DEG C
In the incubation of close shellfish, according to 40-50 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 5-6ppm/d, every day to throw something and feed manufactured feed according to the 0.8-1.0% of close shellfish weight, every day to throw something and feed mixed vitamin according to the 0.005-0.01% of close shellfish weight, day throws something and feeds 4-6 time, evenly throw something and feed, and carry out twice full dose every day and change water
The close shellfish selected through above-mentioned cultivation in December carries out accelerating process, and the shell length of the close shellfish selected is 64 ± 1mm,
Divide pond accelerating by female shellfish and male shellfish, female Bei Chi heats up from 4 DEG C, and heat up every day 0.2-0.5 DEG C, and stablize 3-5d when 5 DEG C, 6 DEG C, 7 DEG C and 8 DEG C, stablize when rising to 9 DEG C and wait to produce, accelerating cycle 40-45d, Xiong Beichi heat up from 4 DEG C, heat up every day 0.2-0.5 DEG C, when rising to 6 DEG C, allowing the temperature of Xiong Beichi remain the low 1-2 DEG C of the temperature of more female Bei Chi, stablizing when rising to 8 DEG C and waiting to produce, impel cycle 40-45d
In accelerating process, according to 80 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 10ppm/d, to throw something and feed yolk according to the standard of 2-6ppm/d, every day to throw something and feed manufactured feed according to the 0.8-1.0% of close shellfish weight, every day to throw something and feed mixed vitamin according to the 0.005-0.01% of close shellfish weight, and day throws something and feeds 4-6 time, evenly throws something and feeds, and every layer of cage places 3-5 only close shellfish in accelerating process, and carry out twice full dose every day and change water
When the genital gland indices of the close shellfish after accelerating reaches more than 10%, brightness value > 50, the red scale value > 30 of female shellfish sexual gland color, the brightness value > 60 of male shellfish sexual gland color, during red scale value > 8, accelerating completes
Dry in the shade the female close shellfish through accelerating 1.5-2h, and male close shellfish is dried in the shade 0.5h, more female close shellfish and male close shellfish are placed in the seawater after Ultraviolet radiation respectively and hasten parturition, and obtain sperm and ovum respectively, the intensity of Ultraviolet radiation is 1.9 × 10 8mwh/L-2.4 × 10 8mwh/L,
Obtain fertilized egg after sperm ovum binding, the stage from fertilized egg to juvenile mollusk all cultivates in Cultivation container, and to seedling after Synchronous Screening, in Cultivation container, throws in adherance,
When juvenile mollusk specification reaches 800-1000 μm, proceed in pond, workshop and keep a full stand of seedings, keep a full stand of seedings time 30-40d, and spat reaches 3-5mm time-division seedling and plunges into the commercial sea, and carries out cage cultivation in sea area.
Described manufactured feed by mass fraction be the sweet potato starch of 5 parts, the sargassum thunbergii powder of the corn starch of 2 parts, the ripe soybean meal of 2 parts and 1 part mixes,
The pantothenic acid of the cobalamin of the vitamin B2 of the vitamin D containing the vitamin A of 46mg/100g, 240 μ g/100g in described mixed vitamin, the vitamin B1 of 59mg/100g, 80mg/100g, the vitamin B6 of 66mg/100g, 129 μ g/100g, the vitamin E of 867mg/100g, the nicotinic acid of 832mg/100g, the folic acid of 13.55mg/100g and 251mg/100g.
Described Synchronous Screening operates according to following steps: when the seedling when 60% reaches more than 200 μm, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container, and former Cultivation container is is normally thrown something and fed and observes, when wherein 60% seedling reach more than 200 μm time, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container.
Compared with the existing technology, tool has the following advantages in the present invention:
The breeding method of Patinopecten yessoensis disclosed in this invention, can allow and only have the Patinopecten yessoensis in an age to breed, the direct saving production of hybrid seeds time limit of Patinopecten yessoensis, epoch breeding can be accelerated, accelerate breed of variety speed, it is through the indoor hot housing of close shellfish, the operation such as incubation and nursery, the sexual maturity time of Patinopecten yessoensis is allowed to shorten to 1 year from 2 years, so just can realize the object improving Patinopecten yessoensis breeding efficiency, its appearance, save time for seeking one, saving of labor, the Patinopecten yessoensis breeding method simultaneously reduced costs provides technical support.
Embodiment
The specific embodiment of the present invention will be described below.
Utilize one age Patinopecten yessoensis carry out the method for breeding, carry out according to following steps:
In October, the water temperature of observation Patinopecten yessoensis culture zone, average temperature as continuous 7 days maritime interior waters is less than the standard equaling 20 DEG C, then from sea area hanging culture Patinopecten yessoensis in select the Patinopecten yessoensis of the long 27 ± 1mm of shell as close shellfish, moved on to indoor to carry out workshop and hang and grow, every layer of cage is placed 6-8 only close shellfish
The cultivation stage of parent shellfish is divided into early stage, mid-term and later stage, the cultivation water temperature of its mid-early stage is 15-20 DEG C, time is 22d, the cultivation water temperature in mid-term is 10-15 DEG C, and the time is 22d, and the cultivation water temperature in later stage is 4-10 DEG C, time is 26d, on average lower the temperature between whole culture period, lower the temperature every day 0.2-0.5 DEG C
In the incubation of close shellfish, according to 40-50 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 5-6ppm/d, throwing something and feeding according to the 0.8-1.0% of close shellfish weight every day, (preferentially select here by mass fraction is the sweet potato starch of 5 parts to manufactured feed, the corn starch of 2 parts, the manufactured feed that the sargassum thunbergii powder of the ripe soybean meal of 2 parts and 1 part mixes), every day (preferentially selects the vitamin A containing 46mg/100g here according to the 0.005-0.01% of close shellfish weight mixed vitamin of throwing something and feeding, the vitamin D of 240 μ g/100g, the vitamin B1 of 59mg/100g, the vitamin B2 of 80mg/100g, the vitamin B6 of 66mg/100g, the cobalamin of 129 μ g/100g, the vitamin E of 867mg/100g, the nicotinic acid of 832mg/100g, 13.55mg/100g folic acid and the mixed vitamin of pantothenic acid of 251mg/100g), the number of times of throwing something and feeding day of above-mentioned all feeds is all 4-6 time, evenly throw something and feed, and carry out twice full dose every day and change water,
Select in December the close shellfish cultivated through above-mentioned steps and carry out accelerating process, the shell length of the close shellfish selected is 64 ± 1mm,
Pond accelerating is divided by female shellfish and male shellfish, female Bei Chi heats up from 4 DEG C, heat up every day 0.2-0.5 DEG C, and at 5 DEG C, 6 DEG C, 7 DEG C and 8 DEG C time stablize 3-5d, stablize when rising to 9 DEG C and wait to produce, the whole accelerating cycle is 40-45d, Xiong Beichi heats up from 4 DEG C, heat up every day 0.2-0.5 DEG C, when rising to 6 DEG C, the temperature of Xiong Beichi is allowed to remain the low 1-2 DEG C of the temperature of more female Bei Chi, that is after 6 DEG C, the temperature of Xiong Beichi can change according to the variations in temperature of female Bei Chi, stablize when the temperature of Damxung Bei Chi rises to 8 DEG C and wait to produce, whole impels cycle 40-45d,
In accelerating process, according to 80 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 10ppm/d, to throw something and feed yolk according to the standard of 2-6ppm/d, every day to throw something and feed manufactured feed according to the 0.8-1.0% of close shellfish weight, and every day to throw something and feed mixed vitamin according to the 0.005-0.01% of close shellfish weight, and the number of times of throwing something and feeding day of above-mentioned all feeds is all 4-6 time, evenly throw something and feed, and in accelerating process, every layer of cage is placed 3-5 only close shellfish, carry out twice full dose every day and change water
When the genital gland indices of the close shellfish after accelerating reaches more than 10%, brightness value > 50, the red scale value > 30 of female shellfish sexual gland color, the brightness value > 60 of male shellfish sexual gland color, during red scale value > 8, explanation accelerating completes, now close Becquerel produces sperm or ovum
Dry in the shade the female close shellfish through accelerating 1.5-2h, and male close shellfish is dried in the shade 0.5h, more female close shellfish and male close shellfish are placed in the seawater after Ultraviolet radiation respectively and hasten parturition, and the intensity of Ultraviolet radiation is 1.9 × 10 8mwh/L-2.4 × 10 8mwh/L, the female close shellfish after process of hastening parturition can produce ovum, and male close shellfish can produce sperm,
Fertilized egg is obtained after sperm ovum binding, stage from fertilized egg to juvenile mollusk all cultivates in Cultivation container, after Synchronous Screening, adherance is thrown in Cultivation container, the juvenile mollusk attachment of Gong hatching, here Synchronous Screening operates according to following steps: when the seedling when 60% reaches more than 200 μm, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container, and former Cultivation container is is normally thrown something and fed and observes, when wherein 60% seedling reach more than 200 μm time, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container,
When the juvenile mollusk specification in Cultivation container reaches 800-1000 μm, proceed in pond, workshop and keep a full stand of seedings, keep a full stand of seedings time 30-40d, and keeping a full stand of seedings here refers to and load in specification 60 object polyethylene mesh bag by juvenile mollusk, each mesh bag puts 2 seedling curtains, often go here and there 10-12 mesh bag, and below mesh bag pendant stone, with reduce mesh bag swing, period of keeping a full stand of seedings throws something and feeds mixed feed, comprise fork limit chrysophyceae, diatom, flat algae etc., and change water day 1 time
Spat reaches 3-5mm time-division seedling and plunges into the commercial sea, and carries out cage cultivation in sea area.

Claims (4)

1. utilize one age Patinopecten yessoensis carry out the method for breeding, it is characterized in that: described method is carried out according to following steps:
In October, the average temperature as continuous 7 days maritime interior waters is less than and equals 20 DEG C, from sea area hanging culture Patinopecten yessoensis in select the Patinopecten yessoensis of the long 27 ± 1mm of shell as close shellfish, carry out hanging between indoor cart growing, every layer of cage placed 6-8 only close shellfish,
The cultivation stage of parent shellfish is divided into early stage, mid-term and later stage, and the cultivation water temperature in early stage is 15-20 DEG C, and the time is 22d, and the cultivation water temperature in mid-term is 10-15 DEG C, time is 22d, and the cultivation water temperature in later stage is 4-10 DEG C, and the time is 26d, on average lower the temperature in the training period, lower the temperature every day 0.2-0.5 DEG C
In the incubation of close shellfish, according to 40-50 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 5-6ppm/d, every day to throw something and feed manufactured feed according to the 0.8-1.0% of close shellfish weight, every day to throw something and feed mixed vitamin according to the 0.005-0.01% of close shellfish weight, day throws something and feeds 4-6 time, evenly throw something and feed, and carry out twice full dose every day and change water
The close shellfish selected through above-mentioned cultivation in December carries out accelerating process, and the shell length of the close shellfish selected is 64 ± 1mm,
Divide pond accelerating by female shellfish and male shellfish, female Bei Chi heats up from 4 DEG C, and heat up every day 0.2-0.5 DEG C, and stablize 3-5d when 5 DEG C, 6 DEG C, 7 DEG C and 8 DEG C, stablize when rising to 9 DEG C and wait to produce, accelerating cycle 40-45d, Xiong Beichi heat up from 4 DEG C, heat up every day 0.2-0.5 DEG C, when rising to 6 DEG C, allowing the temperature of Xiong Beichi remain the low 1-2 DEG C of the temperature of more female Bei Chi, stablizing when rising to 8 DEG C and waiting to produce, impel cycle 40-45d
In accelerating process, according to 80 × 10 4the standard of cell/d is thrown something and fed Nitzschia closterium minutissima, to throw something and feed ocean rhodotorula according to the standard of 10ppm/d, to throw something and feed yolk according to the standard of 2-6ppm/d, every day to throw something and feed manufactured feed according to the 0.8-1.0% of close shellfish weight, every day to throw something and feed mixed vitamin according to the 0.005-0.01% of close shellfish weight, and day throws something and feeds 4-6 time, evenly throws something and feeds, and every layer of cage places 3-5 only close shellfish in accelerating process, and carry out twice full dose every day and change water
When the genital gland indices of the close shellfish after accelerating reaches more than 10%, brightness value > 50, the red scale value > 30 of female shellfish sexual gland color, the brightness value > 60 of male shellfish sexual gland color, during red scale value > 8, accelerating completes
Dry in the shade the female close shellfish through accelerating 1.5-2h, and male close shellfish is dried in the shade 0.5h, more female close shellfish and male close shellfish are placed in the seawater after Ultraviolet radiation respectively and hasten parturition, and obtain sperm and ovum respectively, the intensity of Ultraviolet radiation is 1.9 × 10 8mwh/L-2.4 × 10 8mwh/L,
Obtain fertilized egg after sperm ovum binding, the stage from fertilized egg to juvenile mollusk all cultivates in Cultivation container, and to seedling after Synchronous Screening, in Cultivation container, throws in adherance,
When juvenile mollusk specification reaches 800-1000 μm, proceed in pond, workshop and keep a full stand of seedings, keep a full stand of seedings time 30-40d, and spat reaches 3-5mm time-division seedling and plunges into the commercial sea, and carries out cage cultivation in sea area.
2. according to claim 1 utilize one age Patinopecten yessoensis carry out the method for breeding, it is characterized in that: described manufactured feed by mass fraction be the sweet potato starch of 5 parts, the sargassum thunbergii powder of the corn starch of 2 parts, the ripe soybean meal of 2 parts and 1 part mixes.
3. according to claim 1 utilize one age Patinopecten yessoensis carry out the method for breeding, it is characterized in that: the pantothenic acid of the vitamin E of the vitamin B6 of the vitamin B1 of the vitamin A containing 46mg/100g in described mixed vitamin, the vitamin D of 240 μ g/100g, 59mg/100g, the vitamin B2 of 80mg/100g, 66mg/100g, the cobalamin of 129 μ g/100g, 867mg/100g, the nicotinic acid of 832mg/100g, the folic acid of 13.55mg/100g and 251mg/100g.
4. according to claim 1 utilize one age Patinopecten yessoensis carry out the method for breeding, it is characterized in that: described Synchronous Screening operates according to following steps: when the seedling when 60% reaches more than 200 μm, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container, and former Cultivation container is is normally thrown something and fed and observes, when wherein 60% seedling reach more than 200 μm time, utilize 200 mesh sieve thin,tough silk upper, 300 mesh sieve thin,tough silk under mode to sieve breeding water body, the seedling that 200 order silk cover filterings obtain is moved in new Cultivation container, the seedling that 300 order silk cover filterings obtain moves into former Cultivation container.
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CN106035166A (en) * 2016-06-20 2016-10-26 中国科学院南海海洋研究所 Physical method capable of effectively avoiding self-fertilization of Tridacna sp
CN106614171A (en) * 2015-11-02 2017-05-10 射阳县朱平水产苗种有限公司 High-efficiency river crab seedling growing integrated cultivation method
CN106719182A (en) * 2016-12-02 2017-05-31 獐子岛集团股份有限公司 Patinopecten yessoensis seabed shackle culture method and device
CN111557258A (en) * 2020-06-17 2020-08-21 中国海洋大学 Artificial temperature accumulation and ripening method for bay scallop breeding shells
CN113287552A (en) * 2021-06-29 2021-08-24 广西海洋研究所有限责任公司 Method for synchronously preserving wild pinctada martensii

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CN103168734A (en) * 2013-04-15 2013-06-26 蓬莱市天保海水养殖有限公司 Parent patinopecten yessoensis culturing and hatching method
CN104605171A (en) * 2013-11-04 2015-05-13 中国科学院海洋研究所 Method for using artificial alternative bait for promoting maturity of parent bay scallop

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US20080229437A1 (en) * 2006-02-28 2008-09-18 Lindell Scott R Intergeneric bivalve shellfish hybrid and method for producing
CN101103709A (en) * 2007-08-16 2008-01-16 大连水产学院 Method for breeding double white shell Japanese scallop
CN102100199A (en) * 2010-12-14 2011-06-22 大连獐子岛渔业集团股份有限公司 Method for culturing one-year patinopecten yesoensis
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CN103168734A (en) * 2013-04-15 2013-06-26 蓬莱市天保海水养殖有限公司 Parent patinopecten yessoensis culturing and hatching method
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Publication number Priority date Publication date Assignee Title
CN106614171A (en) * 2015-11-02 2017-05-10 射阳县朱平水产苗种有限公司 High-efficiency river crab seedling growing integrated cultivation method
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CN106035166A (en) * 2016-06-20 2016-10-26 中国科学院南海海洋研究所 Physical method capable of effectively avoiding self-fertilization of Tridacna sp
CN106719182A (en) * 2016-12-02 2017-05-31 獐子岛集团股份有限公司 Patinopecten yessoensis seabed shackle culture method and device
CN111557258A (en) * 2020-06-17 2020-08-21 中国海洋大学 Artificial temperature accumulation and ripening method for bay scallop breeding shells
CN113287552A (en) * 2021-06-29 2021-08-24 广西海洋研究所有限责任公司 Method for synchronously preserving wild pinctada martensii

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