CN103636542B - A kind of Bitterling Welfare indoor artificial propagation methods - Google Patents

A kind of Bitterling Welfare indoor artificial propagation methods Download PDF

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CN103636542B
CN103636542B CN201310696520.6A CN201310696520A CN103636542B CN 103636542 B CN103636542 B CN 103636542B CN 201310696520 A CN201310696520 A CN 201310696520A CN 103636542 B CN103636542 B CN 103636542B
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water
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CN103636542A (en
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徐嘉波
施永海
谢永德
陆根海
张海明
张宗锋
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Shanghai Fisheries Research Institute Shanghai Fisheries Technical Extension Station
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SHANGHAI AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract

一种鳑鲏室内人工繁殖方法,本发明包括亲鱼的强化培育、亲鱼交配、苗种收集和苗种培育,其特征是鳑鲏亲鱼的强化培育采用经过一年池塘养殖的鳑鲏成鱼群体作为亲鱼群体,选择可以见到清新的卵巢轮廓、输卵管延长成管状的雌鱼亲鱼个体和轻压腹部会有乳白色的精液流出的雄鱼亲鱼个体,雌雄比为1:1,在亲鱼养殖池中投放河蚌,1月后拉网收集苗种分入苗种培育池进行培育,苗种培育开口饵料为过80目筛绢网的小型淡水轮虫,往后逐渐补充过60目筛绢网的轮虫、裸腹溞,鱼苗长到全长15mm,投喂过40目筛绢网的轮虫及绕足类,鱼苗全长培育至30mm,可放入池塘进行常规养殖。An indoor artificial propagation method of bream, the invention includes intensive cultivation of broodstock, mating of broodstock, seed collection and seed cultivation, and is characterized in that the intensive cultivation of broodstock of bream adopts the adult fish population of bream that has been cultured in ponds for one year as For broodstock groups, select female broodstock individuals with fresh ovary outlines, oviducts extended into a tubular shape, and male fish broodstock individuals with milky white semen flowing out when lightly pressed on the abdomen. The ratio of male to female is 1:1, and put them in the broodstock breeding pond For mussels, after January, the seeds are collected by pulling nets and divided into seed cultivation pools for cultivation. The opening bait for seed cultivation is small freshwater rotifers that pass through the 80-mesh sieve tulle, and gradually supplement the rotifers that pass through the 60-mesh sieve tulle. rotifers and daphnia, the fry grow to a total length of 15mm, and the rotifers and circlipods that have been passed through a 40-mesh tulle screen are fed. The fry grow to a total length of 30mm and can be put into ponds for conventional breeding.

Description

一种鳑鲏室内人工繁殖方法A kind of indoor artificial propagation method of bream

技术领域technical field

本发明涉及鲤科鱊亚科鳑鲏属鱼类人工繁殖技术,尤其是鳑鲏室内人工繁殖技术。The invention relates to an artificial propagation technique for fishes of the genus Cyprinus subfamily Cyprinidae, in particular to an indoor artificial propagation technique for Cyprinidae.

背景技术Background technique

鳑鲏(Rhodeus), 隶属于鲤形目、鲤科、鱊亚科,是一种小型的杂食性野生淡水鱼类,各大水系皆有分布,尤以长江以南为多,具有特殊的繁殖习性(卵产于河蚌的鳃瓣中)。鳑鲏,杂食性,以藻类为主,食物为水草、高等植物的叶片、藻类、沉淀的有机物、浮游动物、水生昆虫和枝角类等,对于控制富营养化水体产生的藻华现象,改善河道、景观水域、养殖池塘的底质具有积极作用。此外,鳑鲏具有婚姻色,繁殖季节,雄鱼色彩异常鲜艳,腹部呈橘红色,鳍淡红色,吻部及眼眶周缘具珠星,深受中国原生鱼观赏爱好者喜爱,具有观赏价值。同时,鳑鲏亦可作为一味中药材,具有添精补髓,补三焦之火,益脾胃,起痘毒之功效。在科研和养殖生产中,因鳑鲏为小型鱼类,其可作为一些大型肉食性鱼类的饵料鱼;因其繁殖周期较短,亦可开发为动物实验鱼。 Rhodeus , belonging to Cyprinidae, Cyprinidae, and Subfamily Cyprinidae, is a small omnivorous wild freshwater fish. It is distributed in all major water systems, especially in the south of the Yangtze River. It has a special breeding Habit (eggs are laid in the gill flaps of mussels). Seaweed, omnivorous, mainly algae, the food is aquatic plants, leaves of higher plants, algae, sedimentary organic matter, zooplankton, aquatic insects and cladocera, etc. It is useful for controlling algal blooms in eutrophic water bodies and improving river channels. , landscape waters, and the bottom quality of aquaculture ponds have positive effects. In addition, the fish has a marriage color. During the breeding season, the male fish is extremely bright in color, with an orange-red abdomen, light red fins, and pearl stars on the snout and around the eye sockets. It is very popular among Chinese native fish viewing enthusiasts and has ornamental value. At the same time, squid can also be used as a traditional Chinese medicine, which has the effects of adding essence and marrow, nourishing the fire of the triple burner, benefiting the spleen and stomach, and eliminating acne poison. In scientific research and aquaculture production, because the squid is a small fish, it can be used as a bait fish for some large carnivorous fish; because of its short reproductive cycle, it can also be developed as an animal experiment fish.

近年来,水域污染、人为滥捕以及违法电、炸、毒鱼等因素严重影响了鳑鲏的自然资源量,而鳑鲏繁殖力比较弱,其特殊繁殖习性(卵产于河蚌的鳃瓣中)成为开展人工繁殖技术研究的瓶颈,阻碍了对其后续科研、生态、经济价值的开发。目前,国内尚无深入研究人工繁殖技术的报道,因此,鳑鲏人工繁殖技术成为保护鳑鲏种质资源和发展鳑鲏规模化养殖生产的关键,具有十分重要的意义。In recent years, factors such as water pollution, overfishing, illegal electricity, explosives, poisonous fish and other factors have seriously affected the natural resources of the squid, and the fecundity of the squid is relatively weak, and its special breeding habits (eggs are laid on the gill flaps of mussels) Middle) has become a bottleneck in the research of artificial reproduction technology, hindering the development of its subsequent scientific research, ecological and economic value. At present, there is no in-depth research on the artificial propagation technology in China. Therefore, the artificial propagation technology of S. chinensis has become the key to protect the germplasm resources of S. spp.

发明内容Contents of the invention

本发明的目的是提供一种鳑鲏室内集约化人工繁育技术。The purpose of the present invention is to provide a kind of indoor intensive artificial breeding technology of bream.

本发明的技术方案包括鳑鲏亲鱼的强化培育、亲鱼交配、苗种收集和苗种培育步骤;其特征是鳑鲏亲鱼的强化培育采用经过一年池塘养殖的鳑鲏成鱼群体作为亲鱼群体,选择可以见到清新的卵巢轮廓、输卵管延长成管状的雌鱼亲鱼个体和轻压腹部会有乳白色的精液流出的雄鱼亲鱼个体,雌雄比为1:1,要求所挑选亲鱼体格健壮活力好、鳞片和鳍条完整体表光滑无伤痕;亲鱼培育池为20-25 m2室内水泥池,放养密度20-25尾/m2,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,投喂0#虾颗粒饲料,每天投喂2次,每天吸污,每周换水1次;亲鱼培育至4月初,棚内水温已升至15℃以上,在亲鱼培育池中投放河蚌,投放密度为5-10只/m2;雌雄亲鱼自然交配,鳑鲏产卵时,先寻找河蚌,借助细长输卵管插入河蚌鳃水管内,把卵产在鳃内或外套腔中,雌鱼产完卵后,雄鱼立即使其受精,受精卵在蚌体内孵化发育;待河蚌投放1月后,一经观察到亲鱼培育池中有幼鱼集群,用80目的筛绢网拉网收集,拉网操作时下纲绳离池底15-20 cm,收集的幼鱼用盆子连水带苗移入育苗池中,亲鱼返回原池;育苗池为20-25 m2室内水泥池,放养密度1000-2000尾/池,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,采用静水充气方法进行培育,水温20-28℃;育苗用水为淡水,经二级沉淀后,用200目的筛绢网过滤后注入育苗池;苗种培育开口饵料为过80目筛绢网的小型淡水轮虫,轮虫密度为1-2个/ml,上下午各投一次,培育7天后补充过60目筛绢网的轮虫、裸腹溞,增加其适口性;每天吸污清底一次,每天换水一次,换水量为60%;鱼苗长到全长15mm,投喂轮虫及绕足类的幼体,浮游动物捞取后用40目的筛绢进行过滤后投喂,上下午各投一次,每天吸污清底一次,隔天换水一次,换水量为60-70%,培育鱼苗长到全长30mm以上,可放入池塘进行常规养殖。The technical scheme of the present invention includes the steps of intensive cultivation, mating, seed collection, and seed cultivation of the broodstock of the bream; it is characterized in that the intensive cultivation of the broodstock of the bream adopts the adult fish population of the bream that has been cultivated in a pond for one year as the broodstock group, Select female broodstock individuals with fresh ovary outlines, oviducts extended into tubes, and male fish broodstock individuals with milky white semen flowing out when lightly pressed on the abdomen. The ratio of male to female is 1:1. The selected broodstock is required to be strong and vigorous, The scales and fin rays are intact and smooth without scars; the broodstock breeding pond is an indoor cement pond of 20-25 m 2 , the stocking density is 20-25 tails/m 2 , the blower is used to continuously inflate oxygen, and the density of air stones is 0.5-1/ m 2 , fed 0 # shrimp pellets, fed twice a day, sucked up dirt every day, and changed water once a week; broodstock were cultivated until early April, and the water temperature in the shed had risen to above 15°C, and they were put in the broodstock cultivation pond The stocking density of mussels is 5-10/m 2 ; male and female broodstock mate naturally. When oviducts lay eggs, they first look for mussels, insert them into the gill water pipes of mussels with the help of slender oviducts, and lay eggs in the gills or mantle cavity Among them, after the female fish laid eggs, the male fish immediately fertilized them, and the fertilized eggs hatched and developed in the mussels; after the river mussels were released for one month, as soon as juvenile fish clusters were observed in the broodstock breeding pond, 80-mesh sieves were used to screen them. Pull the net to collect. During the operation of pulling the net, the lower line rope is 15-20 cm away from the bottom of the pond. The young fish collected are moved into the nursery pond with water and seedlings, and the broodstock returns to the original pond; the nursery pond is an indoor cement pond of 20-25 m2 . The stocking density is 1000-2000 tails/pond, and the blower is used to continuously inflate and oxygenate. The density of air stones is 0.5-1/m 2 . The static water aeration method is used for cultivation, and the water temperature is 20-28°C. The water for seedling cultivation is fresh water, which has undergone secondary precipitation Finally, filter it with a 200-mesh sieve and inject it into the nursery pond; the opening bait for seedling cultivation is a small freshwater rotifer that has passed through an 80-mesh sieve, and the rotifer density is 1-2/ml. After 7 days, add rotifers and daphnia that passed through a 60-mesh sieve to increase their palatability; absorb the dirt once a day to clear the bottom, change the water once a day, and the water change rate is 60%; The larvae of worms and circlipods, the zooplankton are fished and filtered with 40-mesh sieves, and then fed, once in the morning and afternoon, once a day to absorb the dirt and clean the bottom, and change the water every other day, the water change is 60-70%, The cultivated fish fry grow to a total length of more than 30 mm, and can be put into ponds for conventional culture.

本发明的突出特点是:1)解决了鳑鲏室内人工繁殖技术,相对于鳑鲏需依靠河蚌完成卵黄囊仔鱼期前发育的自然繁殖模式,形成了人工可控性更强的苗种培育技术;2)室内苗种培育相对于自然繁殖成活率明显提高,受自然环境因素影响小;3)解决了人工繁殖和育苗中饵料种类、放养密度、亲鱼幼鱼分离方法等多个关键技术点,形成了操作规程,操作简便,易于接受和推广。The salient features of the present invention are: 1) Solve the indoor artificial breeding technology of scallops, compared with the natural breeding mode of scallops relying on river mussels to complete the development of yolk sac larvae before the stage, forming a seed cultivation with stronger artificial controllability Technology; 2) Compared with natural breeding, the survival rate of indoor seedling cultivation is significantly improved, and is less affected by natural environmental factors; 3) Several key technical points such as bait types, stocking density, and separation methods of broodstock juveniles in artificial breeding and seedling breeding have been solved , forming an operating procedure, which is easy to operate, easy to accept and popularize.

具体实施方式detailed description

本发明的生产技术步骤包括:鳑鲏亲鱼的强化培育、亲鱼交配、胚胎离体、人工孵化和苗种培育。The production technical steps of the invention include: intensified cultivation of the broodstock of the anchovy, mating of the broodstock, isolation of embryos, artificial hatching and seedling cultivation.

一、 亲鱼的强化培育:1. Intensive cultivation of broodstock:

采用经过一年池塘养殖的鳑鲏成鱼群体作为亲鱼群体,选择可以见到清新的卵巢轮廓、输卵管延长成管状的雌鱼亲鱼个体和轻压腹部会有乳白色的精液流出的雄鱼亲鱼个体,雌雄比为1:1,要求所挑选亲鱼体格健壮活力好、鳞片和鳍条完整体表光滑无伤痕。养殖池为20-25 m2室内水泥池,放养密度20-25尾/m2,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,投喂0#虾颗粒饲料,每天投喂2次,每天吸污,每周换水1次。The adult fish populations of scallops that have been cultured in ponds for one year are used as the broodstock population, and the female broodstock individuals with fresh ovarian outlines and oviducts extending into tubes can be seen, and the male fish broodstock individuals with milky white semen flowing out when lightly pressed on the abdomen are selected. The ratio of male to female is 1:1, and the selected broodstock is required to be strong and vigorous, and the scales and fin rays are intact and smooth without scars. The breeding pond is an indoor cement pond of 20-25 m 2 , the stocking density is 20-25 tails/m 2 , the blower is continuously inflated to increase oxygen, the air stone density is 0.5-1/m 2 , and 0 # shrimp pellet feed is fed, every day Feed twice, absorb dirt every day, and change water once a week.

二、 亲鱼交配:2. Parent fish mating:

亲鱼培育至4月初,棚内水温已升至15℃以上,在养殖池中投放河蚌,河蚌投放密度为5-10只/ m2,雌雄亲鱼自然交配,鳑鲏产卵时,先寻找河蚌,借助细长输卵管插入河蚌鳃水管内,把卵产在鳃内或外套腔中,雌鱼产完卵后,雄鱼立即使其受精,受精卵在蚌体内孵化发育。The broodstock was cultivated until the beginning of April, and the water temperature in the shed had risen to above 15°C. The mussels were put into the breeding pond at a density of 5-10 per m 2 , and the male and female broodstock mated naturally. Mussels are inserted into the gills of mussels with the help of slender oviducts, and eggs are laid in the gills or mantle cavity. After the female fish lays eggs, the male fish immediately fertilizes them, and the fertilized eggs hatch and develop in the mussels.

三、 苗种收集:3. Seed collection:

待河蚌投放1月后,一经观察到亲鱼培育池中有幼鱼集群,用80目的筛绢网拉网收集,拉网操作时下纲绳离池底15-20 cm,收集的幼鱼用盆子连水带苗移入育苗池中,亲鱼返回原池。One month after the river mussels were released, once juvenile fish clusters were observed in the broodstock breeding pond, they were collected with an 80-mesh sieve tulle net. When pulling the net, the lower line rope was 15-20 cm away from the bottom of the pond. The collected juvenile fish were collected in a basin. Even the water and the seedlings are moved into the nursery pond, and the broodstock returns to the original pond.

四、 苗种培育:4. Seedling cultivation:

育苗池为20-25 m2室内水泥池,放养密度1000-2000尾/池,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,采用静水充气方法进行培育,水温为自然水温,范围20-28℃;育苗用水为淡水,经二级沉淀后,用200目的筛绢网过滤后注入育苗池;苗种培育开口饵料为过80目筛绢网的小型淡水轮虫,轮虫密度为1-2个/ml,上下午各投一次,培育7天后补充过60目筛绢网的轮虫、裸腹溞,增加其适口性;每天吸污清底一次,每天换水一次,换水量为60%;鱼苗长到全长15mm,投喂轮虫及绕足类的幼体,浮游动物捞取后用40目的筛绢进行过滤后投喂,上下午各投一次,每天吸污清底一次,隔天换水一次,换水量为60-70%,培育鱼苗长到全长30mm以上,可放入池塘进行常规养殖。The nursery pond is an indoor cement pond of 20-25 m 2 , the stocking density is 1000-2000 tails/pond, the blower is used to inflate and oxygenate continuously, the air stone density is 0.5-1/m 2 , and the method of static water aeration is used for cultivation, and the water temperature is natural The water temperature range is 20-28°C; the seedling water is fresh water, after secondary precipitation, it is filtered with a 200-mesh silk screen and then poured into the nursery pool; the opening bait for seedling cultivation is a small freshwater rotifer that has passed through an 80-mesh screen silk screen. The density of worms is 1-2/ml, once in the morning and in the afternoon, and after 7 days of cultivation, add rotifers and daphnia that pass through a 60-mesh sieve to increase their palatability; suck the dirt and clean the bottom once a day, and change the water once a day , the water change rate is 60%; the fish fry grow to a total length of 15mm, and the larvae of rotifers and circumlopods are fed. After the zooplankton is caught, they are filtered with 40-mesh sieves and then fed. Once at the end, change the water once every other day, the water change volume is 60-70%, and the cultivated fish fry grow to a total length of more than 30mm, and can be put into the pond for conventional breeding.

Claims (1)

1.一种鳑鲏室内人工繁殖方法,包括鳑鲏亲鱼的强化培育、亲鱼交配、苗种收集和苗种培育步骤;其特征是鳑鲏亲鱼的强化培育采用经过一年池塘养殖的鳑鲏成鱼群体作为亲鱼群体,选择可以见到清新的卵巢轮廓、输卵管延长成管状的雌鱼亲鱼个体和轻压腹部会有乳白色的精液流出的雄鱼亲鱼个体,雌雄比为1:1,要求所挑选亲鱼体格健壮活力好、鳞片和鳍条完整体表光滑无伤痕;亲鱼培育池为20-25 m2室内水泥池,放养密度20-25尾/m2,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,投喂0#虾颗粒饲料,每天投喂2次,每天吸污,每周换水1次;亲鱼培育至4月初,棚内水温已升至15℃以上,在亲鱼培育池中投放河蚌,投放密度为5-10只/m2;雌雄亲鱼自然交配,鳑鲏产卵时,先寻找河蚌,借助细长输卵管插入河蚌鳃水管内,把卵产在鳃内或外套腔中,雌鱼产完卵后,雄鱼立即使其受精,受精卵在蚌体内孵化发育;待河蚌投放1月后,一经观察到亲鱼培育池中有幼鱼集群,用80目的筛绢网拉网收集,拉网操作时下纲绳离池底15-20 cm,收集的幼鱼用盆子连水带苗移入育苗池中,亲鱼返回原池;育苗池为20-25 m2室内水泥池,放养密度1000-2000尾/池,用鼓风机连续充气增氧,气石密度为0.5-1个/m2,采用静水充气方法进行培育,水温20-28℃;育苗用水为淡水,经二级沉淀后,用200目的筛绢网过滤后注入育苗池;苗种培育开口饵料为过80目筛绢网的小型淡水轮虫,轮虫密度为1-2个/ml,上下午各投一次,培育7天后补充过60目筛绢网的轮虫、裸腹溞,增加其适口性;每天吸污清底一次,每天换水一次,换水量为60%;鱼苗长到全长15mm,投喂轮虫及绕足类的幼体,浮游动物捞取后用40目的筛绢进行过滤后投喂,上下午各投一次,每天吸污清底一次,隔天换水一次,换水量为60-70%,培育鱼苗长到全长30mm以上,可放入池塘进行常规养殖。1. A method for indoor artificial propagation of bream broodstock, comprising steps of intensive cultivation of bream broodstock, mating of broodstock, seed collection and seed cultivation; it is characterized in that the intensive cultivation of bream broodstock adopts bream broodstock grown in ponds for one year As a broodstock group, select female broodstock individuals with fresh ovary outlines, oviducts extended into tubes, and male fish broodstock individuals with milky white semen flowing out when lightly pressed on the abdomen. The ratio of male to female is 1:1, and the selection is required The broodstock is strong and vigorous, with complete scales and fin rays and smooth body surface without scars; the broodstock breeding pond is an indoor cement pond of 20-25 m 2 , the stocking density is 20-25 fish/m 2 , and the blower is used to continuously inflate oxygen to increase the density of air stones. 0.5-1/m 2 , fed 0 # shrimp pellets, fed twice a day, sucked up dirt every day, and changed the water once a week; the broodstock was cultivated until the beginning of April, and the water temperature in the shed had risen to above 15°C. Put mussels in broodstock breeding ponds at a density of 5-10/m 2 ; male and female broodstock mate naturally, and when oviducts lay eggs, first look for mussels, insert them into the gills of mussels with the help of slender oviducts, and lay eggs In the gills or in the mantle cavity, after the female fish lays eggs, the male fish immediately fertilizes them, and the fertilized eggs hatch and develop in the mussels; after one month after the mussels are released, once the juvenile fish clusters are observed in the broodstock breeding pond, Use 80-mesh sieve tulle to pull the net to collect. During the operation of pulling the net, the lower line rope is 15-20 cm away from the bottom of the pond. The young fish collected are moved into the nursery pond with the water and the seedlings, and the broodstock returns to the original pond; the nursery pond is 20-25 cm. m 2 indoor cement ponds, stocking density 1000-2000 tails/pond, continuous aeration and oxygenation with blower, airstone density 0.5-1/m 2 , adopt static water aeration method for cultivation, water temperature 20-28℃; seedling water is Fresh water, after secondary sedimentation, is filtered with 200-mesh silk screen and injected into the seedling pond; the bait for seedling cultivation is small freshwater rotifers passing through 80-mesh screen silk screen, and the density of rotifers is 1-2/ml. Invest once in the afternoon, and after 7 days of cultivation, add rotifers and daphnia that passed through a 60-mesh sieve to increase their palatability; once a day to absorb dirt and clear the bottom, change the water once a day, and the water change rate is 60%; the fry grow to full size. The length is 15mm. Feed the larvae of rotifers and circumlopods. After the zooplankton are fished out, they are filtered with 40-mesh sieves and then fed. They are fed once in the morning and in the afternoon. It is 60-70%, and the cultivated fish fry grow to a total length of more than 30mm, and can be put into ponds for conventional breeding.
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