CN105724843A - Shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens - Google Patents
Shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens Download PDFInfo
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- CN105724843A CN105724843A CN201610196961.3A CN201610196961A CN105724843A CN 105724843 A CN105724843 A CN 105724843A CN 201610196961 A CN201610196961 A CN 201610196961A CN 105724843 A CN105724843 A CN 105724843A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M21/00—Apparatus for the destruction of unwanted vegetation, e.g. weeds
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Abstract
The invention provides a shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens. The shellfish bait microalga concentrated solution provided by the invention is prepared by a method comprising the following steps: culturing shellfish bait microalgae to a later period of a logarithmic growth phase, wherein the alga cell density is 1*10<10>/L-1*10<11>/L; and carrying out vacuum filtering and uniform shaking on the shellfish bait microalgae at the later period of the logarithmic growth phase to obtain the shellfish bait microalga concentrated solution, wherein the alga cell density of the shellfish bait microalga concentrated solution is 10-20 times as much as the alga cell density of the shellfish bait microalga at the later period of the logarithmic growth phase. The shellfish bait microalga concentrated solution provided by the invention is low in production cost and a preparation method is simple and feasible; and damages to shellfish breeding by a brown tide are effectively reduced.
Description
Technical field
The invention belongs to algae cell density field, especially relate to a kind of shellfish bait micro-algae concentrated solution and
Application in pressing down food Chroococcus growth.
Background technology
In recent years, Northern Coastal Region break out with press down food Chroococcus (Aureococcus anophagefferens) be
Main brown tide causes negative effect greatly to China's shellfishery.According to National Bureau of Oceanography's Oceanic disasters
Publication is reported, within 2010, the coastal brown tide area in Hebei province reaches 3350 square kilometres, direct economic loss 2.05
Hundred million yuan.Therefore, need badly set up effective emergence treating method with reduce brown tide shellfishery etc. is caused
Significant damage.3rd country that brown tide occurs after the Shi Ji U.S. of China and South Africa.
Marine shellfish aquaculture yield in China's sea-farming is the highest, and whole economic efficiency is maximum, therefore
The healthy and sustainable development of the mariculture industry whole to China that develop in a healthy way of marine shellfish aquaculture has non-
The most important meaning.But, the reply brown tide harm the most only office to marine shellfish aquaculture of current China
It is limited to monitoring and the research of early warning technology in real time of brown tide, not yet sets up effective emergence treating method.
Press down the reason algae kind that food Chroococcus is brown tide, belong to Chrysophyta (Chrysophyta), sea Chrysophyceae
(Pelagophyceae) a kind of type algae slightly.Press down food Chroococcus and can suppress scallop, Concha Meretricis Seu Cyclinae etc. consumingly
Ingesting of common cultivated shellfish, and then suppress it to grow.In our early-stage Study, send out first
Now part common shellfish bait micro-algae, such as chlorella (Chlorella vulgaris) and Phaeodactylum
(Phaeodactylum tricornutum Bohlin) strong inhibition can press down the life of food Chroococcus in syntaxial system
Long, i.e. shellfish bait micro-algae exists significant gram come into force should pressing down food Chroococcus.
Therefore, based on common shellfish bait micro-algae to press down food Chroococcus exist significant gram come into force should, exploitation
Going out easy and simple to handle, ecological security is good, can the ecocatas-trophe of tide brown to shellfish beds after scale application
Carry out effective emergence treating method, by reducing the harm to shellfish culture of the brown tide, improve shellfish culture
Economic benefit, there is very important actual application value.
Summary of the invention
In view of this, it is contemplated that propose a kind of shellfish bait micro-algae concentrated solution and press down food gold in suppression
Application in the growth of ball algae, effectively reduces the harm to shellfish culture of the brown tide.
For reaching above-mentioned purpose, the technical scheme is that and be achieved in that:
A kind of shellfish bait micro-algae concentrated solution, this shellfish bait micro-algae concentrated solution is by the side comprised the steps
Method prepares: cultivating shellfish bait micro-algae to later stage exponential phase of growth, algae cell density is 1 × 1010Individual/
Rise-1 × 1011Individual/liter, the shellfish bait micro-algae being in later stage exponential phase of growth is carried out vacuum filtration, shakes
Even, obtain shellfish bait micro-algae concentrated solution, the algae cell density of shellfish bait micro-algae concentrated solution is for being in finger
10-20 times of the algae cell density of the shellfish bait micro-algae in number trophophase later stage.
Further, described shellfish bait micro-algae is the one in chlorella or Phaeodactylum.
Further, the algae cell density of described shellfish bait micro-algae concentrated solution is for being in exponential growth after date
10 times of the algae cell density of the shellfish bait micro-algae of phase.
The application of a kind of described shellfish bait micro-algae concentrated solution, described shellfish bait micro-algae concentrated solution exists
Prevent and treat the application in brown tide.
Further, the brown tide of described preventing and treating presses down food Chroococcus growth by suppression and realizes.
Further, described application is when the algae cell density pressing down food Chroococcus reaches 1 × 105-5×106Individual/liter
Time, described bait micro-algae concentrated solution is invested in the waters that cultivated shellfish is intensive, to press down food gold
Ball algae grows.
Further, described shellfish bait micro-algae concentrated solution is thrown in after injecting bait micro-algae concentrated solution slow release instrument
In the waters that cultivated shellfish is intensive.Described bait micro-algae concentrated solution slow release instrument refers to utility model patent
ZL201520754681.0。
Further, the volume of described bait micro-algae concentrated solution slow release instrument is 40-100 liter.
Further, the input density of described bait micro-algae concentrated solution slow release instrument is 10-25/mu.
Further, the release frequency of described bait micro-algae concentrated solution slow release instrument is 9-15 minute/time；Institute
Single release time of the bait micro-algae concentrated solution slow release instrument stated is 1-3 minute；Described bait micro-algae is dense
Contracting liquid delays the replacement frequency of instrument and is 20-40 day/time.
For reaching above-mentioned purpose, the technical scheme is that and be achieved in that:
(1) shellfish bait micro-algae of the present invention concentration cost of manufacture is low, and preparation method is simple,
Effectively reduce the harm to shellfish culture of the brown tide.
(2) shellfish bait micro-algae of the present invention be concentrated in suppression press down food Chroococcus in application cost low,
Easy and simple to handle, ecological security is good, effectively reduces the harm to shellfish culture of the brown tide after scale application,
Improve under the precondition that brown tide occurs, the economic benefit of shellfish culture；Meanwhile, the present invention can be extensive
It is applied to the emergency disposal of the brown tide in shellfish culture marine site.
Detailed description of the invention
Unless otherwise indicated, term used herein is respectively provided with containing of those skilled in the art's routine understanding
Terms more used herein, for the ease of understanding the present invention, have been carried out following definitions by justice.
All of Digital ID, such as pH, temperature, time, concentration, including scope, be all approximation
Value.It is to be understood that, although all add term " about " before all of Digital ID of the clearest and the most definite narration.
Simultaneously it will also be understood that, although the clearest and the most definite narration, reagent described herein is only example, its
Equivalent is known in the art.
The formula of the f/2 culture fluid described in the embodiment of the present invention is as follows: sodium nitrate 75 milligrams, phosphoric acid
Sodium dihydrogen 4.4 milligrams, f/2 trace element mixed solution 1 milliliter, f/2 vitamin mixed solution 1 milliliter,
998 milliliters of the membrane filtration sea water of sterilizing.
Wherein, the formula of f/2 trace element mixed solution: Zn2SO423 milligrams, CuSO4·5H2O 178
Milligram, sodium citrate 3.9 grams, sodium molybdate 7.3 milligrams, sodium ethylene diamine tetracetate 4.35 grams, chlorination
Cobalt 12 milligrams, pure water 1000 milliliters.
Wherein, the formula of f/2 vitamin mixed solution: vitamin B12 (VB12) 0.5 milligram, dimension is raw
Element B1 (VB1) 100 milligrams, biotin (VH) 0.5 milligram, pure water 1000 milliliters.
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1
A kind of shellfish bait micro-algae concentrated solution, this shellfish bait micro-algae concentrated solution is by the side comprised the steps
Method prepares: using f/2 culture fluid to cultivate described chlorella to later stage exponential phase of growth, frustule is close
Degree is 1 × 1011Individual/liter；The chlorella being in later stage exponential phase of growth is carried out vacuum filtration, shakes up,
Obtaining shellfish bait micro-algae concentrated solution, the algae cell density of described shellfish bait micro-algae concentrated solution is
1×1012Individual/liter.
The application of a kind of described shellfish bait micro-algae concentrated solution, described shellfish bait micro-algae concentrated solution exists
Prevent and treat the application in brown tide.The brown tide of described preventing and treating presses down food Chroococcus growth by suppression and realizes.
On on June 8th, 2015 to August 7, use the present invention in Tianjin Hai Sheng aquaculture company limited
Area is the emergency disposal that brown tide is carried out in the Concha Meretricis Seu Cyclinae sea area in 18 mu of 2 age.
Monitor the frustule pressing down food Chroococcus in this Concha Meretricis Seu Cyclinae mariculture area water body on June 8th, 2015 close
Degree reaches 1.3 × 105Individual/liter time, by dense for the microalgae that described shellfish bait micro-algae concentrated solution injects 80 liters
After contracting liquid slow release instrument, it is invested in the waters that cultivated shellfish is intensive, described bait micro-algae concentrated solution slow release
The input density of instrument is 10/mu.The release frequency of described bait micro-algae concentrated solution slow release instrument is 9 points
Clock/time；Single release time of described bait micro-algae concentrated solution slow release instrument is 1 minute.Described bait
It is 30 days/time that microalgae concentrated solution delays the replacement frequency of instrument.
Use the brown tide emergency disposal that Concha Meretricis Seu Cyclinae mariculture area has been carried out 60 days by a definite date by the present invention.Then 8
The moon is randomly drawed 300 Concha Meretricis Seu Cyclinaes on the 8th and weighs.Find through measuring to analyze, use the emergent place of the present invention
The Concha Meretricis Seu Cyclinae in the sea area postponed significantly improves compared with the Concha Meretricis Seu Cyclinae fresh weight carrying out brown tide emergency disposal without the present invention, flat
All improving fresh weight is 8.17 ± 0.76 grams, i.e. fresh weight increment rate reaches 53.51 ± 4.98%, and then effectively drops
The harm to shellfish culture of the low brown tide, improves the yield of shellfish culture under the precondition that brown tide occurs.
On on June 8th, 2015 to August 7, the pressing down of Concha Meretricis Seu Cyclinae culture zone of chlorella concentrated solution is used to eat gold
Ball algae density maintains 1 × 10 always4Individual/liter below, and do not use the Concha Meretricis Seu Cyclinae of chlorella concentrated solution to cultivate
It is to maintain 2.6 × 10 during June 8 to July 17 in 2015 that sea area presses down food Chroococcus density always6
Individual/liter more than, time the highest, density is up to 1.8 × 107Individual/liter, during July 18 to August 7 always
Maintain 3.5 × 105Individual/liter more than.Being primarily due to from July 18 by pressing down food Chroococcus is main breaking out
Brown tide enter phase of decline, but there is also some.
Embodiment 2
A kind of shellfish bait micro-algae concentrated solution, this shellfish bait micro-algae concentrated solution is by the side comprised the steps
Method prepares: using f/2 culture fluid to cultivate described Phaeodactylum to later stage exponential phase of growth, algae is thin
Born of the same parents' density is 3 × 1010Individual/liter；The Phaeodactylum being in later stage exponential phase of growth is carried out vacuum filtration,
Shake up, obtain shellfish bait micro-algae concentrated solution, the algae cell density of described shellfish bait micro-algae concentrated solution
It is 3 × 1011Individual/liter.
The application of a kind of described shellfish bait micro-algae concentrated solution, described shellfish bait micro-algae concentrated solution exists
Prevent and treat the application in brown tide.The brown tide of described preventing and treating presses down food Chroococcus growth by suppression and realizes.
On on May 26th, 2015 to August 12, use the present invention in bay, Changli County, Qinhuangdao, Hebei
Scallop culture area of base is the emergency disposal that brown tide is carried out in the bay scallop culture sea area of 32 mu.
Monitor the algae pressing down food Chroococcus in this bay scallop culture sea area water body on May 26th, 2015
Cell density reaches 3.1 × 106Individual/liter time, described shellfish bait micro-algae concentrated solution is injected 100 liters
After bait micro-algae concentrated solution slow release instrument, it is lifted in the waters that cultivated shellfish is intensive, described bait micro-algae
The input density of concentrated solution slow release instrument is 15/mu.The release of described bait micro-algae concentrated solution slow release instrument
Frequency is 10 minutes/time；Single release time of described bait micro-algae concentrated solution slow release instrument is 2 minutes.
It is 20 days/time that described bait micro-algae concentrated solution delays the replacement frequency of instrument.
Use the brown tide emergency disposal that bay scallop culture district has been carried out 75 days by a definite date by the present invention.Working as
On August 12, in is randomly drawed 300 scallops and weighs.Use the bay scallop of the present invention after testing
Culture zone, scallop average soft tissue growth speed is 10.36 mg/day, and the average dry scallop shell speed of growth is
46.59 mg/day, the soft tissue growth speed being significantly higher than comparison culture zone is 1.53 mg/day, flat
All dry scallop shell speeds of growth are 5.82 mg/day.Therefore, this invention emergency disposal shellfish beds is used
Brown tide can effectively reduce the harm to shellfish culture of the brown tide, improve brown tide occur precondition under shellfish
The yield of cultivation.
On on May 26th, 2015 to August 12, the bay scallop of shellfish bait micro-algae concentrated solution is used to support
The food Chroococcus density that presses down growing district maintains 5.6 × 10 always4Individual/liter below, and do not use shellfish bait
It is that on May 26th, 2015 is to July that the bay scallop culture sea area of microalgae concentrated solution presses down food Chroococcus density
Maintain 8.2 × 10 during 21 days always6Individual/liter more than, time the highest, density is up to 6.9 × 107Individual/liter,
Maintain 8.3 × 10 during July 22 to August 12 always5Individual/liter more than.It is primarily due to from 7
The moon 22 was that the main brown tide broken out enters phase of decline by pressing down food Chroococcus, but there is also some.
Embodiment 3
A kind of shellfish bait micro-algae concentrated solution, this shellfish bait micro-algae concentrated solution is by the side comprised the steps
Method prepares: using f/2 culture fluid to cultivate described Phaeodactylum to later stage exponential phase of growth, algae is thin
Born of the same parents' density is 6 × 1010Individual/liter；The Phaeodactylum being in later stage exponential phase of growth is carried out vacuum filtration,
Shake up, obtain shellfish bait micro-algae concentrated solution, the algae cell density of described shellfish bait micro-algae concentrated solution
It is 6 × 1011Individual/liter.
The application of a kind of described shellfish bait micro-algae concentrated solution, described shellfish bait micro-algae concentrated solution exists
Prevent and treat the application in brown tide.The brown tide of described preventing and treating presses down food Chroococcus growth by suppression and realizes.
On on May 24th, 2015 to August 23, the use present invention is big Shen Tanghai in Pests in Tianjin Binhai New Area
Territory area is the emergency disposal that brown tide is carried out in the Ruditapes philippinarum mariculture area of 66 mu.
On May 24th, 2015 monitor and this Ruditapes philippinarum mariculture area water body pressing down food Chroococcus
Algae cell density reaches 2.8 × 106Individual/liter time, described shellfish bait micro-algae concentrated solution is injected 100 liters
Bait micro-algae concentrated solution slow release instrument after, be lifted in the waters that cultivated shellfish is intensive, described bait is micro-
The input density of algae concentrated solution slow release instrument is 10/mu.Releasing of described bait micro-algae concentrated solution slow release instrument
Putting frequency is 9 minutes/time；Single release time of described bait micro-algae concentrated solution slow release instrument is 1 minute.
It is 30 days/time that described bait micro-algae concentrated solution delays the replacement frequency of instrument.
Use the brown tide emergency disposal that Ruditapes philippinarum mariculture area has been carried out 90 days by a definite date by the present invention.
Results on November 26 Ruditapes philippinarum becomes shellfish then, uses the emergency disposal of shellfish bait micro-algae concentrated solution
The Ruditapes philippinarum mariculture area per mu yield of brown tide reaches 762 kilograms, and does not relatively use shellfish bait micro-algae dense
The Ruditapes philippinarum mariculture area per mu yield of the brown tide of emergency disposal of contracting liquid is 486 kilograms, and per unit area yield improves
56.79%.
On on May 24th, 2015 to August 23, use the Ruditapes philippinarum of shellfish bait micro-algae concentrated solution
The food Chroococcus density that presses down of culture zone maintains 1.23 × 10 always5Individual/liter below, and do not use shellfish bait
Material microalgae concentrated solution Ruditapes philippinarum mariculture area press down food Chroococcus density be on May 24th, 2015 extremely
Maintain 3.7 × 10 during July 20 always6Individual/liter more than, time the highest, density is up to 2.4 × 107Individual
/ liter, maintain 4.9 × 10 during July 21 to August 23 always5Individual/liter more than.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Within the spirit of invention and principle, any modification, equivalent substitution and improvement etc. made, should be included in
Within protection scope of the present invention.
Claims (10)
1. a shellfish bait micro-algae concentrated solution, it is characterised in that: this shellfish bait micro-algae concentrated solution by
The method comprised the steps prepares: cultivate shellfish bait micro-algae to later stage exponential phase of growth, frustule
Density is 1 × 1010Individual/liters-1 × 1011Individual/liter, the shellfish bait micro-algae being in later stage exponential phase of growth is entered
Row vacuum filtration, shaking up, obtain shellfish bait micro-algae concentrated solution, the algae of shellfish bait micro-algae concentrated solution is thin
Born of the same parents' density is 10-20 times of the algae cell density of the shellfish bait micro-algae being in later stage exponential phase of growth.
Shellfish bait micro-algae concentrated solution the most according to claim 1, it is characterised in that: described
Shellfish bait micro-algae is the one in chlorella or Phaeodactylum.
Shellfish bait micro-algae concentrated solution the most according to claim 1 and 2, it is characterised in that: institute
The algae cell density of the shellfish bait micro-algae concentrated solution stated is the shellfish bait being in later stage exponential phase of growth
10 times of the algae cell density of microalgae.
4. the application of the shellfish bait micro-algae concentrated solution according to any one of claim 1-3, its feature
It is: the application in preventing and treating brown tide of the described shellfish bait micro-algae concentrated solution.
The application of shellfish bait micro-algae concentrated solution the most according to claim 4, it is characterised in that:
The brown tide of described preventing and treating presses down food Chroococcus growth by suppression and realizes.
6., according to the application of the shellfish bait micro-algae concentrated solution described in claim 4 or 5, its feature exists
In: described application is when the algae cell density pressing down food Chroococcus reaches 1 × 105-5×106Individual/liter time, by institute
The bait micro-algae concentrated solution stated is invested in the waters that cultivated shellfish is intensive, raw to press down food Chroococcus
Long.
The application of shellfish bait micro-algae concentrated solution the most according to claim 6, it is characterised in that:
Described shellfish bait micro-algae concentrated solution is invested in cultivated shellfish after injecting bait micro-algae concentrated solution slow release instrument
In intensive waters.
The application of shellfish bait micro-algae concentrated solution the most according to claim 7, it is characterised in that:
The volume of described bait micro-algae concentrated solution slow release instrument is 40-100 liter.
The application of shellfish bait micro-algae concentrated solution the most according to claim 7, it is characterised in that:
The input density of described bait micro-algae concentrated solution slow release instrument is 10-25/mu.
The application of shellfish bait micro-algae concentrated solution the most according to claim 7, it is characterised in that:
The release frequency of described bait micro-algae concentrated solution slow release instrument is 9-15 minute/time；Described bait micro-algae
Single release time of concentrated solution slow release instrument is 1-3 minute；Described bait micro-algae concentrated solution delays instrument more
Change frequency and be 20-40 day/time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610196961.3A CN105724843A (en) | 2016-03-30 | 2016-03-30 | Shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610196961.3A CN105724843A (en) | 2016-03-30 | 2016-03-30 | Shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens |
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| CN105724843A true CN105724843A (en) | 2016-07-06 |
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| CN201610196961.3A Pending CN105724843A (en) | 2016-03-30 | 2016-03-30 | Shellfish bait microalga concentrated solution and application thereof to inhibition of growth of aureococcus anophagefferens |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644908A (en) * | 2019-01-11 | 2019-04-19 | 辽宁省海洋水产科学研究院 | A kind of brown damp preventing control method in scallop cage culture zone |
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| CN103897795A (en) * | 2013-03-20 | 2014-07-02 | 浙江大学 | Method for preparing different grades of biological oil by virtue of classified utilization of wet algae biomass |
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| CN204949150U (en) * | 2015-09-24 | 2016-01-13 | 天津农学院 | Little algae concentrate slowly -releasing appearance of bait |
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2016
- 2016-03-30 CN CN201610196961.3A patent/CN105724843A/en active Pending
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|---|---|---|---|---|
| CN103897795A (en) * | 2013-03-20 | 2014-07-02 | 浙江大学 | Method for preparing different grades of biological oil by virtue of classified utilization of wet algae biomass |
| CN104686587A (en) * | 2015-01-26 | 2015-06-10 | 中国海洋大学 | Natural algae inhibiting agent as well as preparation method and application thereof |
| CN204949150U (en) * | 2015-09-24 | 2016-01-13 | 天津农学院 | Little algae concentrate slowly -releasing appearance of bait |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644908A (en) * | 2019-01-11 | 2019-04-19 | 辽宁省海洋水产科学研究院 | A kind of brown damp preventing control method in scallop cage culture zone |
| CN109644908B (en) * | 2019-01-11 | 2021-06-29 | 辽宁省海洋水产科学研究院 | Method for preventing and controlling brown tide in scallop cage culture area |
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Application publication date: 20160706 |