CN108450377A - It is suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk - Google Patents

It is suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk Download PDF

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Publication number
CN108450377A
CN108450377A CN201810069517.4A CN201810069517A CN108450377A CN 108450377 A CN108450377 A CN 108450377A CN 201810069517 A CN201810069517 A CN 201810069517A CN 108450377 A CN108450377 A CN 108450377A
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shi
juvenile mollusk
bait
mixing
culture
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邹杰
杨家林
聂振平
徐艳
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GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF OCEANOLOGY
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GUANGXI ZHUANG AUTONOMOUS REGION INSTITUTE OF OCEANOLOGY
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Virology (AREA)
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  • Biomedical Technology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Food Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

Shi Shi Le(Lutraria sieboldii)For the rare subtidal zone shellfish of coastal areas of southern China, aquaculture is grown rapidly, and seedling is in great demand, and the juvenile mollusk speed of growth more than 1mm shells length is fast, and bait demand is high, the problem of bait insufficient supply often occurs in seedling raising process.The invention filters out that relative individual is medium, nutrition is good and grows fast marine diatom as juvenile mollusk main bait according to Shi Shi Le juvenile mollusk such as water pipe bore from the marine microalgae for be also easy to produce aquation, that is, invent a kind of use pen tip root pipe algae (Rhizosolenia styliformis) and thin and delicate hailian seaweed(Thalathiosira subtilis)As the mixed culture and application process of Shi Shi Le juvenile mollusk main bait, it can be used as main bait or the other Mirco algal foods of collocation fed, meet the high demand of juvenile mollusk bait.

Description

It is suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk
Technical field
The present invention relates to the culture of the suitable mixing Mirco algal food for feeding Shi Shi Le juvenile mollusk and feeding methods, belong to water The bait culture of bivalve shellfish and feeding technology field during production is learned.
Background technology
Shi Shi Le(Lutraria sieboldii)For the rare subtidal zone shellfish of coastal areas of southern China, aquaculture is swift and violent Development, seedling are in great demand.During Shi Shi Le indoor raising of seedling, the young can emerge after juvenile mollusk phase and Tuo Ji, The juvenile mollusk speed of growth more than 1mm shells length is fast, and bait demand is high, and conventional Mirco algal food culture generally requires to be several times as much as nursery Water body could meet, and the lower 3 grades of cultures of normal condition need 3 ~ 4 days, often occur the problem of bait insufficient supply in seedling raising process.Together When otter clam juvenile mollusk individual it is bigger than normal, it is poor to smaller microalgae food habits.According to the bore of ingesting of Shi Shi Le juvenile mollusk oral siphon, Relative individual is medium, nutrition is good marine diatom is filtered out from the marine microalgae for be also easy to produce aquation as juvenile mollusk main bait, By screening find pen tip root pipe algae (Rhizosolenia styliformis) and thin and delicate hailian seaweed(Thalathiosira subtilis)It is suitable as the main bait of Shi Shi Le juvenile mollusk nursing, which mutually stablizes, and can meet Shi Shi Le juvenile mollusk Nutritional need, keep growth rapid, the high demand of juvenile mollusk bait can be met, as main bait or the other microalgae baits of collocation Material is fed, and a kind of feasible bait supply channel is provided for Shi Shi Le juvenile mollusk and related Veneridae juvenile mollusk cultivation.
Invention content
It is an object of the invention to provide a kind of cultures and throwing of the suitable mixing Mirco algal food for feeding Shi Shi Le juvenile mollusk The method of feeding improves survival rate, the growth that Shi Shi Le juvenile mollusk is cultivated by mixing the science culture of Mirco algal food and correctly feeding Rate overcomes the bait that tradition feeds Shi Shi Le juvenile mollusk insufficient, the slow-growing deficiency of juvenile mollusk.
To achieve the above object, this invention takes following technical proposals, it is suitble to the mixing for feeding Shi Shi Le juvenile mollusk micro- The culture of algae bait and feeding method, including following particular technique steps:
(1)Microalgae inoculated and cultured:
Pen tip root pipe algae and thin and delicate hailian seaweed are collected in the puddles of water of seashore beach, and pen tip root pipe algae and thin and delicate hailian seaweed are mixed Be incubated in open container, day intensity of illumination>3000lux is added nature seawater and sterilizes, proportion>1.010 depth of water 1m- Auxin 15g/m is added in seawater for 1.5m, 26 DEG C -32 DEG C of water temperature3With the fermentation anchovy sauce 10g/m boiled3, inoculum concentration is about for the first time The 8%-10% of culture water body is accounted for, wherein pen tip root pipe algae accounting 60%-70% uses 2%CO containing volume fraction2Air pass through Water body is cultivated, culture largely spreads cultivation to when visibility 30cm-40cm as algae continuation, and the inoculum concentration that spreads cultivation accounts for culture water body 20%-30%, culture to visibility<The pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body are obtained when 40cm;
(2)Feeding method:
Take the above-mentioned pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body to feed Shi Shi Le juvenile mollusk, in the juvenile mollusk phase and Shell length >=1mm periods start to feed, and are fed once every 6 ~ 8h, and bait rubs filter with the hands by 300 mesh mesh bags when feeding, and feed big after 3h Amount inflation forms water surface perturbation rolling, while changing 50% or more water, feeds bait water body and accounts for seedling water 5%-8%, holding feeds bait 1.5h-3h breeding water bodies are clarified after material;
The disinfection refers to the pathogen killed using chemically or physically method in water;
The auxin refer to containing 25% nitric acid ammonia, 15% magnesium sulfate, 8% dipotassium hydrogen phosphate, 17% potassium dihydrogen phosphate, 4% calcium chloride, 20% sodium metasilicate, 1% ironic citrate, 0.5% manganese sulfate and surplus are the additive packages of EDTA compositions;
It is 3 that the fermentation anchovy sauce, which refers to by the weight ratio of new iced trash fish and salt,:1 ratio, mixing spontaneous fermentation are formed from after dissolving Anchovy sauce.
The present invention of above-mentioned measure is taken, the culture of pen tip root pipe algae and thin and delicate hailian seaweed is convenient, fast growing, and algae is mutually steady It is fixed, the high nutrition demand of Shi Shi Le juvenile mollusk can be met, keep the growth of Shi Shi Le juvenile mollusk rapid, cultural method of the present invention and throwing Method and process flow science is fed, the mixing microalgae bait of cultivation is applied to actual production, the Shi Shi Le seed of cultivation is healthy and strong, resistance to Transport launches the latent husky rate in sea area up to 99% or more, is the ideal bait of the subtidal zones shellfish such as Shi Shi Le.
Specific implementation mode
Technical scheme of the present invention is described in detail below by way of specific embodiment.
Embodiment 1
(1)It is prepared by auxin:
Formula:25 kilograms of nitric acid ammonia, 15 kilograms of magnesium sulfate, 8 kilograms of dipotassium hydrogen phosphate, 17 kilograms of potassium dihydrogen phosphate, calcium chloride 4 are public Jin, 20 kilograms of sodium metasilicate, 1 kilogram of ironic citrate, 0.5 kilogram and EDTA9.5 kilograms of manganese sulfate;
It takes the raw material in above-mentioned formula to be uniformly mixed, it is spare to be prepared into auxin;
(2)It is prepared by the anchovy sauce that ferments:
90 kilograms of fresh small fish are taken, 30 kilograms of salt are added, uniformly mixed be placed in container seals, ferment under natural conditions It is spare to obtain fermentation anchovy sauce by 30d;
(3)Pen tip root pipe algae and thin and delicate hailian seaweed mixing microdisk electrode:
In August, 2016 establishes the outside scenery osculum pool, water body 300m3, October is using indoor bait workshop culture pen tip root Pipe algae and thin and delicate hailian seaweed mixing Mirco algal food, single water body 15m3, water body disinfection use 10% antiseptic solution containing sodium hypochlorite 100g/m3, chlorine residue or exposure 2d are neutralized after sterilizing 8h.Algae purifies marine microalgae pen tip root pipe algae and thin and delicate extra large chain in coastal waters Algae conservation and uses volume 0.5m according to a conventional method after purification culture3Glass fibre reinforced plastic tub carry out two level culture, condition of culture is: Day intensity of illumination>3000lux is added nature seawater and sterilizes, proportion>1.010, depth of water 1m-1.5m, 26 DEG C -32 DEG C of water temperature, sea Auxin 15g/m is added in water3With the fermentation anchovy sauce 10g/m boiled3, for the first time inoculum concentration account for about culture water body 8%-10%, Wherein pen tip root pipe algae accounting 60%-70%, uses 2%CO containing volume fraction2Air pass through and cultivate water body, culture is to visibility It largely spreads cultivation as algae continuation when 30cm-40cm, the inoculum concentration that spreads cultivation accounts for the 20%-30% of culture water body, wherein inflation uses sieve Hereby air blower and carbon dioxide tank Mixing and aeration, indoor culture 2-3d, pond culture 4-5d;It cultivates to visibility<It is obtained when 40cm To the pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body;
(4)Feeding method:
Take the above-mentioned pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body to feed Shi Shi Le juvenile mollusk, in the juvenile mollusk phase and Shell length >=1mm periods start feed, fed once every 6-8h, when feeding bait using 300 mesh sieve tulles bags rub with the hands filter, feed before add Water is changed in big inflation, feeds after 3h that largely inflation forms water surface perturbation rolling, while changing water 50%, feeds bait water body and accounts for nursery water Body 5%-8%, 1.5h-3h breeding water bodies are clarified after holding feeds bait;
The Shi Shi Le of the present embodiment cultivates water body list pond 20m3, otter clam 250-350 ten thousand is cultivated per pond, takes above-mentioned pen tip root pipe After algae and thin and delicate hailian seaweed mixing Mirco algal food water body feed Shi Shi Le juvenile mollusk, no juvenile mollusk phenomena of mortality, seed health, transport 3h launches latent husky rate intimate 100% when the intermediate rearing of sea area.

Claims (3)

1. being suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk, it is characterised in that the method packet Include following particular technique steps:
(1)Microalgae inoculated and cultured:
Pen tip root pipe algae and thin and delicate hailian seaweed are collected in the puddles of water of seashore beach, and pen tip root pipe algae and thin and delicate hailian seaweed are mixed Be incubated in open container, day intensity of illumination>3000lux is added nature seawater and sterilizes, proportion>1.010 depth of water 1m- Auxin 15g/m is added in seawater for 1.5m, 26 DEG C -32 DEG C of water temperature3With the fermentation anchovy sauce 10g/m boiled3, inoculum concentration is about for the first time The 8%-10% of culture water body is accounted for, wherein pen tip root pipe algae accounting 60%-70% uses 2%CO containing volume fraction2Air pass through Water body is cultivated, culture largely spreads cultivation to when visibility 30cm-40cm as algae continuation, and the inoculum concentration that spreads cultivation accounts for culture water body 20%-30%, culture to visibility<The pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body are obtained when 40cm;
(2)Feeding method:
Take the above-mentioned pipe algae of root containing pen tip and thin and delicate hailian seaweed mixing Mirco algal food water body to feed Shi Shi Le juvenile mollusk, in the juvenile mollusk phase and Start to feed when shell length >=1mm, be fed once every 6-8h, bait rubs filter with the hands by 300 mesh mesh bags when feeding, and feeds a large amount of after 3h Inflation forms water surface perturbation rolling, while changing 50% or more water, feeds bait water body and accounts for seedling water 5%-8%, holding feeds bait 1.5h-3h breeding water bodies are clarified afterwards.
2. being suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk, it is characterised in that the auxin Refer to containing 25% nitric acid ammonia, 15% magnesium sulfate, 8% dipotassium hydrogen phosphate, 17% potassium dihydrogen phosphate, 4% calcium chloride, 20% sodium metasilicate, 1% lemon Lemon acid iron, 0.5% manganese sulfate and surplus are EDTA, the additive being prepared.
3. being suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk, it is characterised in that the fermented fish It is 3 that juice, which refers to by the weight ratio of new iced trash fish and salt,:1 ratio, the anchovy sauce that mixing spontaneous fermentation is formed from after dissolving.
CN201810069517.4A 2018-01-24 2018-01-24 It is suitble to feed culture and the feeding method of the mixing Mirco algal food of Shi Shi Le juvenile mollusk Pending CN108450377A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109258940A (en) * 2018-10-10 2019-01-25 安徽省农业科学院水产研究所 A kind of cultural method of toad tadpoles open-mouthed bait
CN109769685A (en) * 2019-02-03 2019-05-21 福安市冠富农业科技有限公司 A kind of culture zone scene spreads cultivation algae method

Citations (2)

* Cited by examiner, † Cited by third party
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CN102077790A (en) * 2010-11-25 2011-06-01 广西壮族自治区海洋研究所 Artificially breeding method for Lutraria maximae
CN104304099A (en) * 2014-09-01 2015-01-28 广东海洋大学 Method for intermediate cultivation of juvenile lutrariasieboldii reeve in outdoor cement pond

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102077790A (en) * 2010-11-25 2011-06-01 广西壮族自治区海洋研究所 Artificially breeding method for Lutraria maximae
CN104304099A (en) * 2014-09-01 2015-01-28 广东海洋大学 Method for intermediate cultivation of juvenile lutrariasieboldii reeve in outdoor cement pond

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109258940A (en) * 2018-10-10 2019-01-25 安徽省农业科学院水产研究所 A kind of cultural method of toad tadpoles open-mouthed bait
CN109769685A (en) * 2019-02-03 2019-05-21 福安市冠富农业科技有限公司 A kind of culture zone scene spreads cultivation algae method

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Application publication date: 20180828