CN112850913B - Bdellodicus bdellovi and preparation method and use method thereof - Google Patents
Bdellodicus bdellovi and preparation method and use method thereof Download PDFInfo
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- CN112850913B CN112850913B CN202011642097.8A CN202011642097A CN112850913B CN 112850913 B CN112850913 B CN 112850913B CN 202011642097 A CN202011642097 A CN 202011642097A CN 112850913 B CN112850913 B CN 112850913B
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- silicate
- bacteria
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- powder
- leech
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Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 20
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 90
- 241000894006 Bacteria Species 0.000 claims abstract description 87
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 67
- 241000545744 Hirudinea Species 0.000 claims abstract description 51
- 239000000843 powder Substances 0.000 claims abstract description 44
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 39
- 241000604931 Bdellovibrio bacteriovorus Species 0.000 claims abstract description 38
- 239000000440 bentonite Substances 0.000 claims abstract description 37
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 37
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 31
- 239000002131 composite material Substances 0.000 claims abstract description 25
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 claims abstract description 16
- 229940045872 sodium percarbonate Drugs 0.000 claims abstract description 16
- 108091005804 Peptidases Proteins 0.000 claims abstract description 15
- 239000004365 Protease Substances 0.000 claims abstract description 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims description 54
- 239000000203 mixture Substances 0.000 claims description 41
- 239000002994 raw material Substances 0.000 claims description 34
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 31
- 238000005303 weighing Methods 0.000 claims description 25
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 19
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 18
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 18
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 18
- 241001446247 uncultured actinomycete Species 0.000 claims description 18
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 17
- 239000011780 sodium chloride Substances 0.000 claims description 17
- 235000002639 sodium chloride Nutrition 0.000 claims description 17
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 16
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000012452 mother liquor Substances 0.000 claims description 14
- 239000001632 sodium acetate Substances 0.000 claims description 14
- 235000017281 sodium acetate Nutrition 0.000 claims description 14
- 241000228212 Aspergillus Species 0.000 claims description 13
- 238000013329 compounding Methods 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 238000003892 spreading Methods 0.000 claims description 10
- 230000007480 spreading Effects 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 239000006041 probiotic Substances 0.000 claims description 8
- 235000018291 probiotics Nutrition 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 6
- 241000233866 Fungi Species 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 230000000243 photosynthetic effect Effects 0.000 claims description 5
- 230000000529 probiotic effect Effects 0.000 claims description 5
- 102000035195 Peptidases Human genes 0.000 claims description 4
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical group [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 claims description 3
- YGANSGVIUGARFR-UHFFFAOYSA-N dipotassium dioxosilane oxo(oxoalumanyloxy)alumane oxygen(2-) Chemical compound [O--].[K+].[K+].O=[Si]=O.O=[Al]O[Al]=O YGANSGVIUGARFR-UHFFFAOYSA-N 0.000 claims description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 3
- 229910052622 kaolinite Inorganic materials 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229910052627 muscovite Inorganic materials 0.000 claims description 3
- 229910052656 albite Inorganic materials 0.000 claims description 2
- 229910052661 anorthite Inorganic materials 0.000 claims description 2
- GWWPLLOVYSCJIO-UHFFFAOYSA-N dialuminum;calcium;disilicate Chemical compound [Al+3].[Al+3].[Ca+2].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-] GWWPLLOVYSCJIO-UHFFFAOYSA-N 0.000 claims description 2
- 239000011022 opal Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000000454 talc Substances 0.000 claims description 2
- 229910052623 talc Inorganic materials 0.000 claims description 2
- 238000005469 granulation Methods 0.000 claims 1
- 230000003179 granulation Effects 0.000 claims 1
- 229910052652 orthoclase Inorganic materials 0.000 claims 1
- 239000010453 quartz Substances 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 12
- 239000001301 oxygen Substances 0.000 abstract description 12
- 229910052760 oxygen Inorganic materials 0.000 abstract description 12
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 16
- 235000011147 magnesium chloride Nutrition 0.000 description 11
- 239000000126 substance Substances 0.000 description 10
- 239000010802 sludge Substances 0.000 description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- 239000010410 layer Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000002504 physiological saline solution Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 5
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 5
- 235000019270 ammonium chloride Nutrition 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 5
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 108700012359 toxins Proteins 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011241 protective layer Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 241000604933 Bdellovibrio Species 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- 108010007267 Hirudins Proteins 0.000 description 1
- 102000007625 Hirudins Human genes 0.000 description 1
- 241000237903 Hirudo Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 108010055460 bivalirudin Proteins 0.000 description 1
- 229960001500 bivalirudin Drugs 0.000 description 1
- OIRCOABEOLEUMC-GEJPAHFPSA-N bivalirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 OIRCOABEOLEUMC-GEJPAHFPSA-N 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 229940006607 hirudin Drugs 0.000 description 1
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Environmental Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Botany (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bdellovibrio bacteriovorus and a preparation method and a using method thereof. The bdellovibrio bacteriovorus contains 0.4-2% of liquid bacillus, 25-40% of crude silicate, 3-5% of powder EM bacteria, 15-20% of fine silicate, 35-50% of bentonite, 1.5-4.5% of sodium percarbonate, 0.3-1.2% of protease and the balance of normal saline; liquid bacillus is filled in the coarse silicate, powder EM bacteria is filled in the fine silicate, and the coarse silicate and the fine silicate are both coated with bentonite. The preparation method comprises coating powder EM bacteria first and then coating liquid bacillus. The whole pool is directly and evenly thrown for use by a dry method. The leech bottom bacterium is a novel granular composite bottom-modifying bacterium prepared by screening high-efficiency mud-eliminating live bacterium strain compounded silicate and a quick-acting oxygen increasing agent according to the environmental characteristics of the bottom of a leech culture water body, is suitable for breeding and cultivating all leeches, and is particularly suitable for improving and repairing the micro-ecological environment at the bottom of a high-density leech culture pond.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and relates to a hirudo basidioides for leech culture, and a preparation method and a use method thereof.
Background
The leech is a traditional special medicinal aquatic animal in China, and the dried product of the leech can be used as a medicine in traditional Chinese medicine after being soaked. Along with the application and improvement of modern biotechnology, the demand of leeches is increasing year by extraction and processing of hirudin, medical clinical application, and development, research and popularization of Chinese patent medicines. Huge market demand, and wide market prospect for artificial breeding of leeches. The development of leech culture has good market prospect and economic benefit.
Leech cultivation is generally performed in a fresh water pond by using a net cage, the requirement on water quality is high, and cultivation failure can be caused once a water source is polluted or the environment at the bottom of the net cage is deteriorated. The problem that leeches are cultured in net cages in the existing fresh water ponds is troublesome in culture failure, and mainly due to the fact that harmful substances such as residual bait, excrement and the like exist at the bottom of a leech culture water body, sludge is generated at the bottom of the leech culture water body, the bottom of the leech culture water body is blackened and smelled, and the breeding rate and the survival rate of the leeches are reduced. In conclusion, the deterioration of the environment at the bottom of the net cage eventually leads to the failure of cultivation.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the bdellovibrio bacteriovorus capable of decomposing harmful substances at the bottom of the leech culture water body and effectively preventing the bottom of the water body from blackening and smelling, and the preparation method and the use method thereof.
The purpose of the invention is realized by the following technical scheme:
the invention relates to a bdellovibrio bacteriovorus which comprises the following components: 0.4-2% of liquid bacillus, 25-40% of crude silicate, 3-5% of powder EM (effective microorganisms), 15-20% of fine silicate, 35-50% of bentonite, 1.5-4.5% of sodium percarbonate, 0.3-1.2% of protease and the balance of normal saline; the liquid bacillus is filled in the crude silicate, and bentonite is coated outside the crude silicate; the powder EM bacteria are filled in the fine silicate, and bentonite is coated outside the fine silicate; the crude silicate is granular silicate with the diameter of 1-2 mm; the fine silicate is a silicate having a diameter of 0.15mm or less (100 mesh or more).
The percentages stated in the invention are mass percentages.
Further, the silicate may be orthoclase (K [ AlSi3O8 ]), albite (Na [ AlSi3O8 ]), kaolinite (Al 4[ Si4O10] [ OH ] 8), anorthite (Ca [ Al2Si2O8 ]), talc (Mg 3Si4O10[ OH ] 2), muscovite (KAl 2[ AlSi3O10] [ OH ] 2), quartz (SiO 2), opal (SiO 2. NH 2O), or the like.
Further, the liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus natto contains more than 100 hundred million live bacteria.
Furthermore, the powder EM bacteria contain rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae and aspergillus actinomycete.
Furthermore, the powder EM bacteria are prepared by the following method: mixing and compounding 10-20 parts of rhodopseudomonas palustris, 5-10 parts of lactobacillus acidophilus, 2-4 parts of brewing yeast and 1-2 parts of actinomycete aspergillus to obtain a strain for later use; pouring the obtained strain and a culture solution containing ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water into a fermentation tank according to the volume ratio of 1-10, keeping the temperature at 23-28 ℃, and carrying out anaerobic fermentation culture for 4-10 days to obtain a composite strain mother solution; and freeze-drying the obtained composite bacterium mother liquor to obtain the powder EM bacterium.
Further, in the culture fluid, ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose, distilled water = 100: 8360 (mass ratio).
The applicable temperature of the bdellovibrio bacteriovorus is 10-40 ℃, and the applicable water body pH value is 6.0-10.0.
The preparation method of the bdellovibrio bacteriovorus comprises the following steps:
s1, weighing fine silicate with the diameter of less than 0.15mm according to the formula amount, uniformly spreading the fine silicate, uniformly spraying powder EM bacteria of the formula amount on the fine silicate, and uniformly mixing the raw materials; then weighing bentonite raw material with the formula amount of 1/3-1/2, and mixing the bentonite raw material with the raw material mixture to obtain EM-coated bacteria;
s2, weighing granular crude silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading, uniformly spraying liquid bacillus with the formula amount on the crude silicate, and uniformly mixing the raw materials;
s3, uniformly mixing the mixture obtained in the S2 with the EM-coated bacteria obtained in the S1;
s4, weighing 2/3-1/2 of the formula amount of the bentonite raw material, and mixing the bentonite raw material with the mixture obtained in the S3;
s5, weighing the sodium percarbonate raw material and the protease according to the formula amount, and uniformly stirring and mixing;
and S6, mixing the mixture obtained in the step S4 and the mixture obtained in the step S5 again to obtain a mixture, then putting the mixture into a stirrer, adding physiological saline with the formula amount, fully stirring and mixing to meet the granulating requirement, and finally putting the mixture into a granulator to prepare granules.
Further, the powder EM bacteria is prepared by the following steps:
(1) Preparation of the culture solution
Taking ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water, wherein the mass ratio of the ammonia chloride to the sodium bicarbonate to the dipotassium hydrogen phosphate to the sodium acetate to the magnesium chloride to the sodium chloride to the glucose is as follows: distilled water =75: 8360 mixing, dissolving completely, and making into culture solution;
(2) Preparation of composite probiotic preparation
Taking rhodopseudomonas palustris in photosynthetic bacteria, the strain content is 30 multiplied by 10 8 cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 10 8 cfu/ml or more; the content of Saccharomyces cerevisiae in the yeast is 20 × 10 8 cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 10 6 cfu/ml or more; mixing and compounding 10-20 parts of rhodopseudomonas palustris, 5-10 parts of lactobacillus acidophilus, 2-4 parts of brewing yeast and 1-2 parts of actinomycete aspergillus to obtain a strain for later use;
(3) Preparation of composite bacterium mother liquor
Pouring the strain obtained in the step (2) and the culture solution obtained in the step (1) into a fermentation tank according to the volume ratio of 1;
(4) And (4) performing low-temperature freeze drying on the composite bacterium mother liquor obtained in the step (3) to obtain composite strain freeze-dried powder, namely powder EM bacteria.
The bdellovibrio bacteriovorus is used by the following method: the whole pool is directly and evenly thrown by a dry method.
The dosage is as follows: 30-50 g (preferably 40 g) of the bdellovibrio bacteriovorus is directly thrown every square meter and is thrown every 4-10 days; when the bottom material of the leech culture water body is seriously deteriorated, the leech bottom bacterium can be used doubly, namely 60 to 100 grams (preferably 80 grams) of the leech bottom bacterium is used per square meter.
The bdellovibrio bacteriovorus is suitable for breeding and cultivating all leeches, and is particularly suitable for improving and repairing the micro-ecological environment at the bottom of a high-density leech cultivating pond.
The invention has the beneficial effects that:
the invention relates to a leech bottom bacterium, which is a novel granular composite bottom-modified bacterium prepared by carefully screening high-efficiency live slime-eliminating bacteria aiming at the environmental characteristics of the bottom of a leech culture water body and compounding substances such as crude silicate with extremely strong ion adsorption capacity and a quick-acting oxygen increasing agent.
The bdellovibrio bacteriovorus is prepared by adopting a multilayer embedding method, namely, EM bacteria are firstly embedded (powder EM bacteria are filled in pores of fine silicate, and bentonite is coated outside), then liquid bacillus is embedded (liquid bacillus is filled in pores of crude silicate, and bentonite is coated outside), and finally sodium percarbonate and protease are coated. Wherein the EM is anaerobe, afraid of oxygen, and embedded in the innermost layer; liquid bacillus needs oxygen and is embedded in the secondary outer layer, sodium percarbonate is an oxygen increasing agent, and proteolytic enzyme is coated on the outermost layer. When the bdellovibrio bacteriovorus is put into a water body for use, firstly, the outermost layer of the oxygen increasing powder sodium percarbonate reacts with water to produce oxygen, then, the liquid bacillus (net bottom bacteria) is released to play a role, the sludge at the bottom of the water body is rapidly decomposed and converted, and after about 30 minutes, the oxygen is consumed, and the EM bacteria begin to release to play a role.
The bdellovibrio bacteriovorus is a multi-layer embedding and low-temperature extruding machine-made granular preparation, has high survival rate of live bacteria, is directly thrown and rapidly sunk, is cracked layer by layer when meeting water, has obvious effect, prevents ozone generated by an oxygen increasing agent after hydrolysis from influencing the activity of beneficial strains from the process design, obviously improves the using effect of the product and is very convenient to use.
The leech bottom bacteria can effectively permeate to the bottom of the leech culture water body, can quickly improve the oxidation-reduction potential and dissolved oxygen of the pond bottom, and can efficiently decompose harmful substances such as residual bait, excrement and the like at the bottom of the leech culture water body, so that sludge at the bottom of the leech culture water body is quickly decomposed, converted and eliminated; meanwhile, an ammonifying bacteria protective layer can be formed at the bottom of the leech culture water body, and the bottom of the leech culture water body can be effectively prevented from blackening and smelling after the leech culture water body is regularly used.
The bdellovibrio bacteriovorus disclosed by the invention can effectively adsorb harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and algal toxin at the bottom of the pool, stabilize the pH value of a water body, supplement trace elements, improve the transparency of the water body and achieve the purposes of improving the substrate and purifying the water quality.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The bdellovibrio bacteriovorus comprises the following components: 0.4% of liquid bacillus, 40% of crude silicate, 3% of powder EM (effective microorganisms), 15% of fine silicate, 35% of bentonite, 2.5% of sodium percarbonate, 1.2% of protease and 2.9% of physiological saline; the liquid bacillus is filled in the crude silicate, and bentonite is coated outside the crude silicate; the powder EM bacteria are filled in the fine silicate, and bentonite is coated outside the fine silicate; the crude silicate is granular silicate with the diameter of 1-2 mm; the fine silicate is a silicate having a diameter of 0.15mm or less (100 mesh or more). The silicate is kaolinite (Al 4[ Si4O10] [ OH ] 8). The above percentages are mass percentages.
The liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus contains more than 100 hundred million live bacteria.
The powder EM bacteria contain rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae and actinomycete aspergillus; the powder EM is prepared by the following method: mixing and compounding 10 parts of rhodopseudomonas palustris, 5 parts of lactobacillus acidophilus, 2 parts of brewing yeast and 1 part of actinomycete aspergillus, namely compounding according to the mass ratio of 10; pouring the obtained strain and a culture solution containing ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water into a fermentation tank according to the volume ratio of 1; and freeze-drying the obtained composite bacterium mother liquor to obtain the powder EM bacterium.
The applicable temperature of the bdellovibrio bacteriovorus is 10-40 ℃, and the applicable water body pH value is 6.0-10.0.
The preparation method of the bdellovibrio bacteriovorus of the embodiment is carried out according to the following steps:
s1, weighing fine silicate with the diameter of less than 0.15mm according to the formula amount, uniformly spreading the fine silicate, uniformly spraying powder EM bacteria of the formula amount on the fine silicate, and uniformly mixing the raw materials; then weighing 1/3 of the formula amount of the bentonite raw material, and mixing the bentonite raw material with the raw material mixture to obtain EM bacteria coated;
s2, weighing granular crude silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading, uniformly spraying liquid bacillus with the formula amount on the crude silicate, and uniformly mixing the raw materials;
s3, uniformly mixing the mixture obtained in the S2 with the EM-coated bacteria obtained in the S1;
s4, weighing 2/3 of the formula amount of the bentonite raw material, and mixing the bentonite raw material with the mixture obtained in the S3;
s5, weighing sodium percarbonate raw materials and proteolytic enzyme according to the formula, and uniformly stirring and mixing;
and S6, mixing the mixture obtained in the step S4 and the mixture obtained in the step S5 again to obtain a mixture, then putting the mixture into a stirrer, adding physiological saline with the formula amount, fully stirring and mixing to meet the granulating requirement, and finally putting the mixture into a granulator to prepare granules.
The powder EM bacteria in the embodiment is prepared by the following steps:
(1) Preparation of the culture solution
0.75 g of ammonium chloride (NH 4 Cl), 1 g of sodium bicarbonate (NaHCO 3), 0.5 g of dipotassium hydrogen phosphate (K2 HPO 4), 1.75 g of sodium acetate (CH 3 COONa), 0.4 g of magnesium chloride (MgCl 2), 2 g of sodium chloride (NaCl) and 10 g of glucose (C6H 12O 6) are added with 8360 g of distilled water and fully dissolved to prepare a culture solution for later use.
(2) Preparation of composite probiotic preparation
Taking rhodopseudomonas palustris in photosynthetic bacteria, the strain content is 30 multiplied by 10 8 cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 10 8 cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 10 8 cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 10 6 cfu/ml or more; according to 10 parts of marsh red fake sheetMixing and compounding bacillus, 5 parts of lactobacillus acidophilus, 2 parts of brewing yeast and 1 part of actinomycete aspergillus to prepare a strain for later use;
(3) Preparation of composite bacterium mother liquor
Pouring the strains obtained in the step (2) and the culture solution obtained in the step (1) into a fermentation tank according to the volume ratio of 1;
(4) And (4) drying the composite bacterium mother liquor obtained in the step (3) to obtain powder EM bacteria.
Example 2
The bdellovibrio bacteriovorus comprises the following components: 2% of liquid bacillus, 25% of crude silicate, 5% of powder EM (effective microorganisms), 20% of fine silicate, 40% of bentonite, 4.5% of sodium percarbonate, 0.3% of protease and 3.2% of physiological saline; the liquid bacillus is filled in the crude silicate, and bentonite is coated outside the crude silicate; the powder EM bacteria are filled in the fine silicate, and bentonite is coated outside the fine silicate; the crude silicate is granular silicate with the diameter of 1-2 mm; the fine silicate is a silicate having a diameter of 0.15mm or less (100 mesh or more). The silicate is orthoclase (K [ AlSi3O8 ]). The above percentages are mass percentages.
The liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus natto contains more than 100 hundred million live bacteria.
The powder EM bacteria contain rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae and actinomycete aspergillus; the powder EM is prepared by the following method: mixing and compounding 15 parts of rhodopseudomonas palustris, 7.5 parts of lactobacillus acidophilus, 3 parts of brewing yeast and 1.5 parts of actinomycete aspergillus to prepare a strain for later use; pouring the obtained strain and a culture solution containing ammonia chloride, sodium bicarbonate, dipotassium phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water into a fermentation tank according to the volume ratio of 1 to 10, keeping the temperature at 28 ℃, and performing anaerobic fermentation culture for 4 days to obtain a composite strain mother solution; and freeze-drying the obtained composite bacterium mother liquor to obtain the powder EM bacterium.
The applicable temperature of the bdellovibrio bacteriovorus is 10-40 ℃, and the applicable water body pH value is 6.0-10.0.
The preparation method of the bdellovibrio bacteriovorus of the embodiment is carried out according to the following steps:
s1, weighing fine silicate with the diameter of less than 0.15mm according to the formula amount, uniformly spreading the fine silicate, uniformly spraying powder EM bacteria of the formula amount on the fine silicate, and uniformly mixing the raw materials; weighing bentonite raw material which is more than 1/3 (less than 1/2) of the formula amount and mixing with the raw material mixture to obtain EM-coated bacteria;
s2, weighing granular crude silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading, uniformly spraying liquid bacillus with the formula amount on the crude silicate, and uniformly mixing the raw materials;
s3, uniformly mixing the mixture obtained in the S2 with the EM-coated bacteria obtained in the S1;
s4, weighing the rest bentonite raw material which is less than 2/3 (more than 1/2) in formula amount and mixing with the mixture obtained in the S3;
s5, weighing the sodium percarbonate raw material and the protease according to the formula amount, and uniformly stirring and mixing;
and S6, mixing the mixture obtained in the step S4 and the mixture obtained in the step S5 again to obtain a mixture, then putting the mixture into a stirrer, adding physiological saline with the formula amount, fully stirring and mixing to meet the granulating requirement, and finally putting the mixture into a granulator to prepare granules.
The powder EM bacteria in the embodiment is prepared by the following steps:
(1) Preparation of the culture solution
Mixing ammonium chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water, and adding ammonium chloride (NH) 4 Cl), sodium bicarbonate (NaHCO) 3 ) Dipotassium hydrogen phosphate (K) 2 HPO 4 ) Sodium acetate (CH) 3 COONa magnesium chloride (MgCl) 2 ) Sodium chloride (NaCl), glucose (C) 6 H 12 O 6 ) Distilled water (H) 2 O) =75: 8360 mixing, dissolving completely, and making into culture solution;
(2) Preparation of composite probiotic preparation
Taking rhodopseudomonas palustris in photosynthetic bacteria, the strain content is 30 multiplied by 10 8 cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 10 8 cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 10 8 cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 10 6 cfu/ml or more; mixing and compounding 15 parts of rhodopseudomonas palustris, 7.5 parts of lactobacillus acidophilus, 3 parts of brewing yeast and 1.5 parts of actinomycete aspergillus to obtain a strain for later use;
(3) Preparation of composite bacterium mother liquor
Pouring the strain obtained in the step (2) and the culture solution obtained in the step (1) into a fermentation tank according to the volume ratio of 1;
(4) And (4) drying the composite bacterium mother liquor obtained in the step (3) to obtain powder EM bacteria.
Example 3
The bdellovibrio bacteriovorus comprises the following components: 0.5% of liquid bacillus, 35% of crude silicate, 4% of powder EM (effective microorganisms), 17% of fine silicate, 36.5% of bentonite, 1.5% of sodium percarbonate, 0.5% of protease and 5% of normal saline; the liquid bacillus is filled in the crude silicate, and bentonite is coated outside the crude silicate; the powder EM bacteria are filled in the fine silicate, and bentonite is coated outside the fine silicate; the crude silicate is granular silicate with the diameter of 1-2 mm; the fine silicate is a silicate having a diameter of 0.15mm or less (100 mesh or more). The silicate is muscovite (KAl 2[ AlSi3O10] [ OH ] 2). The above percentages are mass percentages.
The liquid bacillus adopts liquid bacillus natto, and each ml of the liquid bacillus natto contains more than 100 hundred million live bacteria.
The powder EM contains rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae and aspergillus actinomycete; the powder EM is prepared by the following method: mixing and compounding 20 parts of rhodopseudomonas palustris, 10 parts of lactobacillus acidophilus, 4 parts of brewing yeast and 2 parts of actinomycete aspergillus to prepare a strain for later use; pouring the obtained strain and a culture solution containing ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water into a fermentation tank according to the volume ratio of 1; and freeze-drying the obtained composite bacterium mother liquor to obtain the powder EM bacterium.
The applicable temperature of the bdellovibrio bacteriovorus is 10-40 ℃, and the pH value of the applicable water body is 6.0-10.0.
The preparation method of the bdellovibrio bacteriovorus of the embodiment is carried out according to the following steps:
s1, weighing fine silicate with the diameter of less than 0.15mm according to the formula amount, uniformly spreading the fine silicate, uniformly spraying powder EM bacteria of the formula amount on the fine silicate, and uniformly mixing the raw materials; then weighing 1/3 of the formula amount of the bentonite raw material, and mixing the bentonite raw material with the raw material mixture to obtain EM bacteria coated;
s2, weighing granular crude silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading, uniformly spraying liquid bacillus with the formula amount on the crude silicate, and uniformly mixing the raw materials;
s3, uniformly mixing the mixture obtained in the S2 with the EM-coated bacteria obtained in the S1;
s4, weighing 2/3 of the formula amount of the bentonite raw material, and mixing the bentonite raw material with the mixture obtained in the S3;
s5, weighing sodium percarbonate raw materials and proteolytic enzyme according to the formula, and uniformly stirring and mixing;
and S6, mixing the mixture obtained in the step S4 and the mixture obtained in the step S5 again to obtain a mixture, then putting the mixture into a stirrer, adding physiological saline with the formula amount, fully stirring and mixing to meet the granulating requirement, and finally putting the mixture into a granulator to prepare granules.
The powder EM bacteria in the embodiment is prepared by the following steps:
(1) Preparation of the culture solution
Mixing ammonium chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water, and adding ammonium chloride (NH) 4 Cl), sodium bicarbonate (NaHCO) 3 ) Dipotassium hydrogen phosphate (K) 2 HPO 4 ) Sodium acetate (CH) 3 COONa magnesium chloride (MgCl) 2 ) Sodium chloride (NaCl), glucose (C) 6 H 12 O 6 ) Distilled water (H) 2 O) =75: 8360, mixing, and dissolving completely to obtain culture solution;
(2) Preparation of composite probiotic preparation
Taking rhodopseudomonas palustris in photosynthetic bacteria, the strain content is 30 multiplied by 10 8 cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 10 8 cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 10 8 cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 10 6 cfu/ml or more; mixing and compounding 20 parts of rhodopseudomonas palustris, 10 parts of lactobacillus acidophilus, 4 parts of brewing yeast and 2 parts of actinomycete aspergillus to obtain a strain for later use;
(3) Preparation of composite bacteria mother liquor
Pouring the strain obtained in the step (2) and the culture solution obtained in the step (1) into a fermentation tank according to the volume ratio of 1;
(4) And (4) drying the composite bacterium mother liquor obtained in the step (3) to obtain powder EM bacteria.
The efficacy, main ingredients, and use notes of the hirulog products prepared in examples 1 to 3 are as follows:
[ EFFECT ] A Chinese medicinal preparation
1. And (3) increasing dissolved oxygen: the oxidation-reduction potential and dissolved oxygen at the bottom of the leech culture water body are improved, the mass propagation of aerobic probiotics is promoted, and the growth of anaerobic harmful bacteria is inhibited;
2. improving the substrate: degrading residual bait, excrement, animal and plant corpses and organic debris at the bottom of the leech culture water body;
3. purifying water quality: effectively adsorbing harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxins and the like at the bottom of the tank;
4. stabilizing the pH value of the water body, supplementing trace elements, keeping the proportion balance of probiotics and plankton in the water, and inhibiting the propagation and growth of harmful algae;
5. improving the transparency of the water body and ensuring the normal operation of the photosynthesis of the plankton in the water.
[ PROPERTIES ]
1. The product is a multi-layer embedding machine granule preparation, has high survival rate of viable bacteria, can be directly thrown, and is convenient to use.
2. The product has wide temperature range, and the living bacteria can grow and propagate at water temperature of 10-40 deg.C.
3. The product has strong adaptability and strong tolerance to chemical disinfection drugs, insecticidal drugs, antibiotics and the like.
4. The product is suitable for water body with pH of 6.0-10, and is suitable for various leech culture water bodies.
[ physicochemical Properties ] yellow-white granules.
[ Main Components ] Bacillus subtilis, nitrifying bacteria, sulfurating bacteria, protease, silicate, bentonite, sodium humate, bioflocculant, EDTA, and sodium percarbonate.
[ content of active ingredients ] each gram contains 10 × 108 probiotics.
The application range is suitable for the breeding and cultivation of all leeches, and is particularly suitable for improving and restoring the micro-ecological environment at the bottom of a high-density leech cultivation pond.
[ USE ] the whole pool is directly thrown evenly by a dry method.
[ dosage ] 40 g of the product is directly thrown every square meter and every 7-10 days, and can be used twice when the water body substrate of the leech culture is seriously deteriorated.
[ PACKAGE ] 5 kg/bag.
[ STORAGE ] is preserved in a cool, dry and ventilated place for 24 months.
Application examples
Application case of bdellovibrio in cleaning sludge at bottom of net cage in net cage culture leeches
Treating a water body: an application test of pond substrate and water quality improvement of the leech cage culture pond in the new village and town of the dormitory of Jiangsu Suqian city is carried out, a leech culture pond with the area of 7.5 mu is taken, the pond is in the north-south direction, and the water drainage and irrigation are convenient. A net cage with the specification of 50 meters in length, 3 meters in width and 0.9 meter in height is placed in the aquaculture pond, and the specification of the net sheet is 80 meshes.
Leech young seedlings are stocked in 1 day 6 months in 2020, the specification of the leech young seedlings put into the net cage is 50000 pieces/kg, and the stocking density is 3000 pieces/square meter. Then, the bdellovibrio bacteriovorus of the embodiment 1 is used for carrying out a cleaning treatment experiment of sludge at the bottom of the net cage, and the observation period of the experiment is 45 days. The first 10 days: the preparation is used for 1 time every 5 days, and 40 g of bdellovibrio bacteriovorus is directly thrown on water surface of each square meter; 35 days after the last treatment: the preparation is used for 1 time every 5 days, and 80 g of bdellovibrio bacteriovorus is directly thrown on water surface of each square meter (the substrate of the leech culture water body is seriously deteriorated and is used twice). During the experiment, snail baits are fed every day.
Collecting a water sample in the net cage and the bottom environment of a pond outside the net cage to perform physical and chemical index measurement when leech fries are put in the pond, and obtaining data as follows: 0.6mg/L of ammonia nitrogen, 0.05mg/L of nitrite nitrogen, 0.02mg/L of hydrogen sulfide, 8.7 of pH value and 25 cm of sludge at the bottom of the pond outside the net cage.
The sludge treatment period of the bottom of the net cage is 45 days by applying the bdellovibrio bacteriovorus. After the treatment is finished, collecting a water sample in the net cage and the bottom environment of the pond outside the net cage to perform physical and chemical index measurement, and obtaining data as follows: 0.2mg/L of ammonia nitrogen, 0.01mg/L of nitrite nitrogen, 0.01mg/L of hydrogen sulfide, 8.5 of pH value and 10 cm of sludge at the bottom of the pond outside the net cage.
The implementation case shows that the bdellovibrio bacteriovorus has obvious improvement effect on the environment of the net cage culture pond, and because the bdellovibrio bacteriovorus is solid particles, the bdellovibrio bacteriovorus has the advantages of rapid bottom settlement, effective permeation to the bottom of the net cage and strong slow release effect, sludge at the bottom in the net cage is rapidly decomposed, converted and eliminated; meanwhile, an ammonifying bacteria protective layer can be formed at the bottom of the net cage, and the bottom of the net cage can be effectively prevented from blackening and smelling after being used regularly. And harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxins and the like can be effectively adsorbed, the pH value of the water body is stabilized, trace elements are supplemented, the transparency of the water body is improved, and the purposes of improving the substrate and purifying the water quality are achieved. The problem of the troublesome that the cultivation fails because the environment at the bottom of the net cage deteriorates in the process of culturing the leeches in the net cage of the fresh water pond is solved.
Claims (5)
1. The bdellovibrio bacteriovorus is characterized by comprising the following components: 0.4-2% of liquid bacillus, 25-40% of crude silicate, 3-5% of powder EM (effective microorganisms), 15-20% of fine silicate, 35-50% of bentonite, 1.5-4.5% of sodium percarbonate, 0.3-1.2% of protease and the balance of normal saline; the liquid bacillus is filled in the crude silicate, and bentonite is coated outside the crude silicate; the powder EM bacteria are filled in the fine silicate, and bentonite is coated outside the fine silicate; the crude silicate is granular silicate with the diameter of 1-2 mm; the fine silicate is silicate with the diameter of less than 0.15 mm;
the bdellovibrio bacteriovorus is prepared by the following steps:
s1, weighing fine silicate with the diameter of less than 0.15mm according to the formula amount, uniformly spreading, uniformly spraying powder EM bacteria on the fine silicate according to the formula amount, and uniformly mixing to obtain a mixture; then weighing bentonite raw material with the formula amount of 1/3-1/2, and mixing the bentonite raw material with the mixture to obtain EM-coated bacteria;
s2, weighing granular crude silicate with the diameter of 1-2mm according to the formula amount, uniformly spreading, uniformly spraying liquid bacillus with the formula amount on the crude silicate, and uniformly mixing;
s3, uniformly mixing the mixture obtained in the S2 with the EM-coated bacteria obtained in the S1;
s4, weighing 2/3-1/2 formula amount of bentonite raw material and mixing with the mixture obtained in the S3;
s5, weighing sodium percarbonate raw materials and proteolytic enzyme according to the formula, and uniformly stirring and mixing;
s6, mixing the mixture obtained in the step S4 and the mixture obtained in the step S5 again to obtain a mixture, then putting the mixture into a stirrer, adding a formula amount of normal saline, fully stirring and mixing to meet the granulation requirement, finally putting the mixture into a granulator to prepare granules, and preparing to obtain the bdellovibrio bacteriovorus which is embedded with EM bacteria, embedded with liquid bacillus and coated with sodium percarbonate and protease;
the powder EM is prepared by the following method:
(1) Preparation of the culture solution
Taking ammonia chloride, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose and distilled water, wherein the weight percentages of the ammonia chloride, the sodium bicarbonate, the dipotassium hydrogen phosphate, the sodium acetate, the magnesium chloride, the sodium chloride and the glucose are as follows: distilled water =75: 8360, mixing, and dissolving completely to obtain culture solution;
(2) Preparation of composite probiotic preparation
Taking rhodopseudomonas palustris in photosynthetic bacteria, the strain content is 30 multiplied by 10 8 cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 10 8 cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 10 8 cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 10 6 cfu/ml or more; mixing and compounding 10-20 parts of rhodopseudomonas palustris, 5-10 parts of lactobacillus acidophilus, 2-4 parts of brewing yeast and 1-2 parts of actinomycete aspergillus to prepare a strain for later use;
(3) Preparation of composite bacterium mother liquor
Pouring the strain obtained in the step (2) and the culture solution obtained in the step (1) into a fermentation tank according to the volume ratio of 1;
(4) And (4) performing low-temperature freeze drying on the composite bacterium mother liquor obtained in the step (3) to obtain composite strain freeze-dried powder, namely powder EM.
2. The bdellovibrio bacteriovorus according to claim 1, wherein the silicate is orthoclase, albite, kaolinite, anorthite, talc, muscovite, quartz or opal.
3. The bdellovibrio bacteriovorus according to claim 1 or 2, wherein the liquid bacillus is liquid bacillus natto, and each ml of the bacillus bacteriovorus contains more than 100 hundred million viable bacteria.
4. A method for using the bdellovibrio bacteriovorus according to any one of claims 1 to 3, characterized in that the whole pond is directly and evenly thrown by dry method; the dosage is as follows: 30-50 g of the bdellovibrio bacteriovorus is directly thrown every square meter and is thrown once every 4-10 days; when the bottom material of the leech culture water body is seriously deteriorated, the leech culture water body is used twice, namely 60 to 100 grams of the leech bottom bacteria are used per square meter and directly thrown.
5. The method for using the bdellovibrio bacteriovorus according to claim 4, wherein 40 g of the bdellovibrio bacteriovorus is directly thrown per square meter; when the bottom material of the leech culture water body is seriously deteriorated, the leech culture water body is used in double, namely 80 grams of the leech bottom bacteria are used per square meter and directly thrown.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1702045A (en) * | 2004-05-24 | 2005-11-30 | 上海泓宝绿色水产科技发展有限公司 | Preparation of bacterium for cleaning pool bottom and method for rehabilitation of aquaculture environment |
| CN103011425A (en) * | 2012-12-27 | 2013-04-03 | 上海泓宝绿色水产科技发展有限公司 | Granular composite bottom treatment bacterium and preparation method thereof |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104671435A (en) * | 2015-02-09 | 2015-06-03 | 华南理工大学 | Environment-friendly cultivation water controlled-release oxygen-increasing agent and preparation method thereof |
| CN109430579A (en) * | 2018-12-10 | 2019-03-08 | 上海泓宝绿色水产股份有限公司 | EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1702045A (en) * | 2004-05-24 | 2005-11-30 | 上海泓宝绿色水产科技发展有限公司 | Preparation of bacterium for cleaning pool bottom and method for rehabilitation of aquaculture environment |
| CN103011425A (en) * | 2012-12-27 | 2013-04-03 | 上海泓宝绿色水产科技发展有限公司 | Granular composite bottom treatment bacterium and preparation method thereof |
| CN104232497A (en) * | 2013-06-19 | 2014-12-24 | 上海泓宝绿色水产科技发展有限公司 | EM flora preparation for improving water quality by compounding a plurality of strains and preparation method of EM flora preparation |
| CN104671435A (en) * | 2015-02-09 | 2015-06-03 | 华南理工大学 | Environment-friendly cultivation water controlled-release oxygen-increasing agent and preparation method thereof |
| CN109430579A (en) * | 2018-12-10 | 2019-03-08 | 上海泓宝绿色水产股份有限公司 | EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method |
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