CN111434623A - Tablet for improving culture environment of culture pond and preparation method and application thereof - Google Patents
Tablet for improving culture environment of culture pond and preparation method and application thereof Download PDFInfo
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- CN111434623A CN111434623A CN201910029200.2A CN201910029200A CN111434623A CN 111434623 A CN111434623 A CN 111434623A CN 201910029200 A CN201910029200 A CN 201910029200A CN 111434623 A CN111434623 A CN 111434623A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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Abstract
The invention belongs to the field of aquaculture, and discloses a tablet for improving a culture environment of a culture pond, and a preparation method and application thereof. The tablet comprises the following raw materials: a Bacillus subtilis solid culture and a Bacillus amyloliquefaciens solid culture. Further comprising: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder, maltodextrin and magnesium stearate. Researches show that in the process of preparing the tablet from the raw materials, the tablet can be directly tabletted without a granulating process, can quickly improve the oxidation-reduction potential and dissolved oxygen of the bottom of the tank, decompose sludge at the bottom of the tank, degrade residual baits, feces, animal and plant corpses and organic debris at the bottom of the tank, effectively adsorb harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the tank, reduce the acidity of a water body, stabilize the pH value of the water body, supplement trace elements, improve the transparency of the water body and achieve the purposes of improving the substrate and purifying the water quality.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a tablet for improving a culture environment of a culture pond, and a preparation method and application thereof.
The culture pond culture environment comprises but is not limited to: the quality of the bottom of the culture pond and the quality of the water quality of the culture pond.
The culture pond comprises a seedling culture pond and a culture pond.
Background
With the development of the breeding industry, the drug feed additives such as antibiotics, olaquindox, quinolones, heavy metal elements (such as copper, zinc and the like) and the like are widely applied in the world. The drug feed additive has obvious effects on preventing and treating diseases, promoting animal growth and the like, but the side effects caused by the drug resistance, toxicity, causing of pathogenic bacteria, causing of 'three causes' (teratogenesis, carcinogenesis and mutagenesis) and the like are gradually revealed, and in addition, farmers put a large amount of chemical fertilizers into water and discharge domestic sewage and industrial wastewater, so that nitrogen, phosphorus and heavy metals exceed the standard, the eutrophication of lakes far exceeds the bearing capacity of rivers and lakes, the rivers and lakes cannot have the opportunity of rest and health, and the aquatic ecosystem is seriously damaged. The problems of residual toxicity, environmental pollution and threat to human health are now very concerned by breeding workers and health workers in various countries around the world. With the wide application of animal microecologics in livestock raising and the like, the application of the animal microecologics in aquaculture industry draws more and more attention of people, and the good effect of the animal microecologics is proved by a large number of experiments and production practices. The microecological preparation has the advantages of small investment, large income, no residual toxicity, no drug resistance, no environmental pollution and the like, fully shows the superiority of utilizing the microecological control technology, and inevitably becomes the development direction of the aquaculture industry in the future.
Disclosure of Invention
For the reasons, the applicant finds a new tablet capable of changing the bottom quality and the water quality of a culture pond through years of research, and the tablet comprises the following raw materials: a Bacillus subtilis solid culture and a Bacillus amyloliquefaciens solid culture. Further comprising: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder, maltodextrin and magnesium stearate. Researches show that in the process of preparing the tablet from the raw materials, the tablet can be directly tabletted without a granulating process, can quickly improve the oxidation-reduction potential and dissolved oxygen of the bottom of the tank, decompose sludge at the bottom of the tank, degrade residual baits, feces, animal and plant corpses and organic debris at the bottom of the tank, effectively adsorb harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the tank, reduce the acidity of a water body, stabilize the pH value of the water body, supplement trace elements, improve the transparency of the water body and achieve the purposes of improving the substrate and purifying the water quality.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a tablet, the raw materials of which comprise: a Bacillus subtilis solid culture and a Bacillus amyloliquefaciens solid culture.
The tablet comprises the following raw materials: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder and zeolite powder.
The tablet comprises the following raw materials: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder, maltodextrin and magnesium stearate.
The tablet is prepared from the following raw materials:
4-5% of bacillus subtilis solid culture, 4-5% of bacillus amyloliquefaciens solid culture, 40-60% of anhydrous sodium sulphate, 10-20% of sodium humate, 10-19% of dolomite powder, 2-5% of zeolite powder, 6-8% of maltodextrin and 1.0% of magnesium stearate.
The tablet is prepared from the following raw materials:
4% of bacillus subtilis solid culture, 4% of bacillus amyloliquefaciens solid culture, 50% of anhydrous sodium sulphate, 13% of sodium humate, 15% of dolomite powder, 5% of zeolite powder, 8% of maltodextrin and 1.0% of magnesium stearate.
The tablet is prepared from the following raw materials:
5% of bacillus subtilis solid culture, 4% of bacillus amyloliquefaciens solid culture, 40% of anhydrous sodium sulphate, 20% of sodium humate, 19% of dolomite powder, 5% of zeolite powder, 6% of maltodextrin and 1% of magnesium stearate.
The tablet is prepared from the following raw materials:
5% of bacillus subtilis solid culture, 5% of bacillus amyloliquefaciens solid culture, 60% of anhydrous sodium sulphate, 10% of sodium humate, 10% of dolomite powder, 2% of zeolite powder, 7% of maltodextrin and 1% of magnesium stearate.
The preparation method of the tablet comprises the following steps:
mixing Bacillus subtilis solid culture, Bacillus amyloliquefaciens solid culture, Natrii sulfas, sodium humate, dolomite powder, zeolite powder, and maltodextrin, adding magnesium stearate, mixing, and tabletting to obtain tablet.
The tablet is used for improving the quality of the bottom of a culture pond.
The tablet is used for improving the quality of pond water in a culture pond.
The raw material of the tablet of the present invention is provided by Shenyao (Xianghe) science and technology Co., Ltd, and the purchase date is 1 month and 10 days in 2017.
The viable count of the bacillus subtilis solid culture is 150 cfu/g.
The viable count of the bacillus amyloliquefaciens solid culture is 150 cfu/g.
The tablet prepared by the method is a gray tablet; the content of active ingredients is 10cfu/g of viable count. The method is suitable for all breeding ponds and culture ponds of marine and freshwater aquatic animals in south and north, and is particularly suitable for microbial improvement of the pond bottom of a multi-sludge pond.
The use method of the tablet type composite bottom modifying bacterium comprises the following steps: the whole pool is directly and evenly sprinkled by a dry method.
The dosage of the tablet type composite bottom modifying bacterium is as follows:
1. the fertilizer is used after pond cleaning and before stocking, namely water is added for 10-20 cm after 5 days of pond cleaning and disinfection, and 200g of fertilizer is used per mu according to the aging condition of pond bottom, and the fertilizer is directly and uniformly sprayed on the pond bottom.
2. The use in the breeding process: the dosage of the fertilizer is 80-200 g per mu according to the aging condition of the pond bottom.
The invention has the beneficial effects that:
the tablet type composite bottom modifying bacterium is a novel granular type composite bottom modifying bacterium which is formed by screening high-efficiency water purification viable bacteria, compounding ore powder with extremely strong adsorption capacity and indirect oxygenation and substrate modification substances. The product is in the form of tablet, has simple preparation process, high survival rate of viable bacteria, direct sprinkling, rapid bottom sinking, obvious effect and convenient use. The oxidation-reduction potential and dissolved oxygen at the bottom of the pool can be rapidly improved, sludge at the bottom of the pool can be decomposed, residual bait, excrement, animal and plant corpses and organic debris at the bottom of the pool can be degraded, ammonia nitrogen, nitrite, hydrogen sulfide, algal toxins and other harmful substances at the bottom of the pool can be effectively adsorbed, the acidity of the water body can be reduced, the pH value of the water body can be stabilized, trace elements can be supplemented, the transparency of the water body can be improved, and the purposes of improving the bottom and purifying the water quality can be achieved.
The tablet type composite bottom modifying bacterium has the following effects:
1. improving the substrate: the residual bait, the excrement, the animal and plant corpses and the organic debris at the bottom of the pool are degraded.
2. And increasing dissolved oxygen, namely increasing the oxidation-reduction potential and the dissolved oxygen at the bottom of the pool, promoting the mass propagation of aerobic probiotics and inhibiting the growth of anaerobic harmful bacteria.
3. Purifying water quality, namely effectively adsorbing harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the tank.
4. Improving the transparency of the water body and ensuring the normal operation of the photosynthesis of the plankton in the water.
5. Stabilizing the pH value of the water body, supplementing trace elements, keeping the proportion balance of probiotics and plankton in the water, and inhibiting the propagation and growth of harmful algae.
The tablet type composite bottom modifying bacterium has the following characteristics:
1. the product is a mechanically pressed granule preparation, has high survival rate of viable bacteria, can be directly sprayed, and is convenient for use.
2. The product has wide temperature range, and the living bacteria can grow and propagate at water temperature of 10-40 deg.C.
3. The product is suitable for water body with pH of 6.0-10, and is suitable for various aquaculture water bodies.
4. The product has strong adaptability and strong tolerance to chemical disinfection drugs, insecticidal drugs, antibiotics and the like.
Detailed Description
The present invention will be described in further detail with reference to examples, but the present invention is not limited to these specific embodiments.
Example 1
A tablet type composite bottom modifying bacterium has the following formula (namely 1000 kg of raw materials required by the production of the composite bottom modifying bacterium): 40kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 500kg of anhydrous sodium sulphate, 130kg of sodium humate, 150kg of dolomite powder, 50kg of zeolite powder, 80kg of maltodextrin and 10kg of magnesium stearate.
The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:
(1) 40kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 500kg of anhydrous sodium sulphate, 130kg of sodium humate, 150kg of dolomite powder, 50kg of zeolite powder and 80kg of maltodextrin, and uniformly mixing.
(2) 10kg of magnesium stearate is taken.
(3) And (3) mixing and stacking the mixture obtained in the first step and the product obtained in the second step again at one position, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare tablets.
The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:
the water body to be treated is fresh water shrimp pond with 10 mu of water surface, ammonia nitrogen is 1.0 mg/L, nitrite nitrogen is 0.5mg/m L, and hydrogen sulfide is 0.2mg/m L.
The treatment period is 6 months, the product is used 1 time every 10 days in the first 3 months, and 100g of the product is used on each mu of water surface (the water depth is 1 meter); the product is applied once every 7 days in the last 3 months, and 200g of the product is applied to each mu of water surface (the water depth is 1 meter).
And after the treatment is finished, determining a water sample, wherein the ammonia nitrogen is less than 0.05 mg/L, the nitrite nitrogen is 0.05mg/m L, and the hydrogen sulfide is 0.05mg/m L.
Example 2
A tablet type composite bottom modifying bacterium has the following formula (namely 1000 kg of raw materials required by the production of the composite bottom modifying bacterium): 50kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 400kg of anhydrous sodium sulphate, 200kg of sodium humate, 190kg of dolomite powder, 50kg of zeolite powder, 60kg of maltodextrin and 10kg of magnesium stearate.
The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:
(1) 50kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 400kg of anhydrous sodium sulphate, 200kg of sodium humate, 190kg of dolomite powder, 50kg of zeolite powder and 60kg of maltodextrin, and uniformly mixing.
(2) 10kg of magnesium stearate is taken.
(3) And (3) mixing and stacking the mixture obtained in the first step and the product obtained in the second step again at one position, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare tablets.
The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:
the water body to be treated is fresh water fishpond with 25 mu of water surface, ammonia nitrogen is 1.8 mg/L, nitrite nitrogen is 0.4mg/m L, and hydrogen sulfide is 0.2mg/m L.
The treatment period is 6 months, the product is used for 1 time every 10 months in the first 3 months, and 250g of the product is used for each mu of water surface (the water depth is 1 meter); the product is applied once every 7 days in the last 3 months, and 200g of the product is applied to each mu of water surface (the water depth is 1 meter).
And after the treatment is finished, determining a water sample, wherein the ammonia nitrogen is less than 0.05 mg/L, the nitrite nitrogen is 0.01mg/m L, and the hydrogen sulfide is 0.05mg/m L.
Example 3
A tablet type composite bottom modifying bacterium has the following formula (namely 1000 kg of raw materials required by the production of the composite bottom modifying bacterium): 50kg of bacillus subtilis solid culture, 50kg of bacillus amyloliquefaciens solid culture, 600kg of anhydrous sodium sulphate, 100kg of sodium humate, 100kg of dolomite powder, 20kg of zeolite powder, 70kg of maltodextrin and 10kg of magnesium stearate.
The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:
(1) 50kg of bacillus subtilis solid culture, 50kg of bacillus amyloliquefaciens solid culture, 600kg of anhydrous sodium sulphate, 100kg of sodium humate, 100kg of dolomite powder, 20kg of zeolite powder and 70kg of maltodextrin, and uniformly mixing.
(2) 10kg of magnesium stearate is taken.
(3) And (3) mixing and stacking the mixture obtained in the first step and the product obtained in the second step again at one position, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare tablets.
The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:
the water body to be treated is a seawater shrimp pond with the surface of 80 mu of water, the ammonia nitrogen is 1.4 mg/L, the nitrite nitrogen is 0.5mg/m L, and the hydrogen sulfide is 0.25mg/m L.
The treatment period is 6 months, the product is used 1 time every 10 days in the first 3 months, and 100g of the product is used on each mu of water surface (the water depth is 1 meter); the product is applied every 7 days for 3 months later, and 200g per mu of water surface (water depth 1 m)
And after the treatment is finished, determining a water sample, wherein the ammonia nitrogen is less than 0.1 mg/L, the nitrite nitrogen is 0.01mg/m L, and the hydrogen sulfide is 0.02mg/m L.
The above embodiment shows that the tablet of the invention has obvious improvement effect on aquaculture water environment, and the tablet has strong slow release effect, can treat different harmful substances in different water depths, so that the high-efficiency effect is achieved, and the treatment effect is good.
Claims (10)
1. A tablet characterized by: the raw materials of the tablet comprise: a Bacillus subtilis solid culture and a Bacillus amyloliquefaciens solid culture.
2. A tablet according to claim 1, characterised in that the tablet material comprises: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder and zeolite powder.
3. A tablet according to claim 1, characterised in that the tablet material comprises: bacillus subtilis solid culture, bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder, maltodextrin and magnesium stearate.
4. A tablet according to claim 3, characterised in that it is prepared from the following raw materials:
4-5% of bacillus subtilis solid culture, 4-5% of bacillus amyloliquefaciens solid culture, 40-60% of anhydrous sodium sulphate, 10-20% of sodium humate, 10-19% of dolomite powder, 2-5% of zeolite powder, 6-8% of maltodextrin and 1.0% of magnesium stearate.
5. A tablet according to claim 3, characterised in that it is prepared from the following raw materials:
4% of bacillus subtilis solid culture, 4% of bacillus amyloliquefaciens solid culture, 50% of anhydrous sodium sulphate, 13% of sodium humate, 15% of dolomite powder, 5% of zeolite powder, 8% of maltodextrin and 1.0% of magnesium stearate.
6. A tablet according to claim 3, characterised in that it is prepared from the following raw materials:
5% of bacillus subtilis solid culture, 4% of bacillus amyloliquefaciens solid culture, 40% of anhydrous sodium sulphate, 20% of sodium humate, 19% of dolomite powder, 5% of zeolite powder, 6% of maltodextrin and 1% of magnesium stearate.
7. A tablet according to claim 3, characterised in that it is prepared from the following raw materials:
5% of bacillus subtilis solid culture, 5% of bacillus amyloliquefaciens solid culture, 60% of anhydrous sodium sulphate, 10% of sodium humate, 10% of dolomite powder, 2% of zeolite powder, 7% of maltodextrin and 1% of magnesium stearate.
8. A tablet according to any one of claims 1 to 7, characterised in that it is prepared by a process comprising:
mixing Bacillus subtilis solid culture, Bacillus amyloliquefaciens solid culture, Natrii sulfas, sodium humate, dolomite powder, zeolite powder, and maltodextrin, adding magnesium stearate, mixing, and tabletting to obtain tablet.
9. A tablet according to any one of claims 1 to 7, characterised in that it is used for improving the bottom quality of a culture pond.
10. A tablet according to any one of claims 1 to 7, characterised in that it is used for improving the pond water quality of a culture pond.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910029200.2A CN111434623A (en) | 2019-01-12 | 2019-01-12 | Tablet for improving culture environment of culture pond and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910029200.2A CN111434623A (en) | 2019-01-12 | 2019-01-12 | Tablet for improving culture environment of culture pond and preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113754218A (en) * | 2021-10-09 | 2021-12-07 | 广州金水动物保健品有限公司 | Tablet of composite bottom modifying bacteria and preparation method thereof |
| CN113998788A (en) * | 2020-12-25 | 2022-02-01 | 江门市澳保生物科技有限公司 | Pond bottom material modifier and preparation method and application thereof |
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| CN113998788A (en) * | 2020-12-25 | 2022-02-01 | 江门市澳保生物科技有限公司 | Pond bottom material modifier and preparation method and application thereof |
| CN113754218A (en) * | 2021-10-09 | 2021-12-07 | 广州金水动物保健品有限公司 | Tablet of composite bottom modifying bacteria and preparation method thereof |
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Application publication date: 20200721 |