KR101481766B1 - Soil conditioner containing microorganism - Google Patents

Abstract

The present invention relates to a microorganism soil conditional for improving crop culturing soil, which contains a microorganism preparation containing 5-15 wt% of a carbon source, 2-10 wt% of a nitrogen source, 0.5-3 wt% of an organic acid, 20-30 wt% of a microorganism culturing fluid, and the remaining amount of water; and a carrier in which a microorganism which is contained in the microorganism preparation is fixed. The soil conditional can reduce salt stress of a soil.

Description

{Soil conditioner containing microorganism}

The present invention relates to a microorganism-containing soil conditioner for improving soil over-fertilization due to excessive fertilization and continuous cultivation of crops.

The rate of cultivation of various vegetables in Korea is 39.3% of the total area of the field, and it is highly dependent on the fact that it occupies 46% of total vegetable consumption. Facility cultivation is continuously cultivated in the same package, and nutrients are excessively accumulated.

In addition, most cultivated land uses excess fertilizer to increase crop yield per unit area, so that excess nutrients are present in the soil even if it is not cultivated. In particular, organic farming using mainly organic fertilizer is difficult to control the amount of fertilizer supplied compared with the method using a single chemical fertilizer, so that some components that are not used by the plant remain in the soil often causing salt disorder.

Salinity disorder is a phenomenon that salt is excessively accumulated in the soil to inhibit the growth of crops and reduce the yield. It causes inhibition of nutrient absorption of plants or uneven nutrient absorption phenomenon. As a result, the germination of the crop is slowed down, and physiological phenomena such as immature, death, growth and growth inhibition, leaf blight and inhibition of root development appear.

On the other hand, there are many places where crops are cultivated by using paddy soils in a large part of the soil cultivation or the cultivation of the facilities. In such cases, there is a problem caused by excessive humidification, salt leaching due to poor drainage, Is more likely to appear.

Measures against these salt obstacles can be made through correct fertilization, turn-over, abandonment or desalination, but it is difficult because of the economic burden. In addition, when pests such as nematodes are generated in the soil and subjected to fresh water treatment or fumigation treatment, useful soil microorganisms as well as pathogens are also killed or the vitality is greatly reduced.

In order to solve the above-mentioned problems, the present invention provides a microorganism-containing soil remediation agent capable of reducing the concentration of excessively accumulated salts in a crop cultivation soil, thereby reducing damage to plants due to salt obstruction.

In order to solve the above-mentioned problems, the present invention provides a microbial soil conditioner capable of reducing the salt concentration of the soil and thereby reducing the damage of the crop by increasing the vitality of the soil microbes and adsorbing and separating the excess salts do.

The present invention relates to a microorganism preparation comprising 5 to 15% by weight of a carbon source, 2 to 10% by weight of a nitrogen source, 0.5 to 3% by weight of an organic acid, 20 to 30% by weight of a microorganism culture solution, And a carrier to which the microorganisms contained in the microbial agent are fixed. The present invention also provides a microbial soil conditioner for improving crop cultivation soil.

According to one embodiment of the present invention, the carrier may include organic and inorganic materials in a weight ratio of 60 to 70: 30 to 40.

According to another embodiment of the present invention, the carrier may be composed of calcium silicate, rice bran and main anchoring.

According to another embodiment of the present invention, the microbial agent may be supported on the carrier in an amount of 8 to 20% by weight based on the total weight of the microbial soil conditioner.

According to another embodiment of the present invention, the carbon source is preferably molasses, dextrin, or a mixture thereof.

According to another embodiment of the present invention, it is preferable that the nitrogen source is whey protein, soybean protein, or a mixture thereof.

According to another embodiment of the present invention, it is preferable that the organic acid is at least one selected from the group consisting of acetic acid, citric acid, lactic acid, and humic acid.

According to another embodiment of the present invention, the microbial culture solution is prepared by cultivating at least one member selected from the group consisting of Bacillus subtilis, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Photosynthetic bacteria .

According to another embodiment of the present invention, the microorganism culture solution comprises Bacillus subtilis; And at least one selected from the group consisting of Lactobacillus acidophilus, Saccharomyces cerevisiae and photosynthetic bacteria are preferably mixed and cultured.

The microbial soil amendment agent according to the present invention contains a carbon source, a nitrogen source and inorganic nutrients for growth of microorganisms, and oxygen and nutrients are supplied smoothly, so that a soil environment in which microorganisms can grow well after soil inoculation can be created.

In addition, the microbial soil conditioner according to the present invention can exhibit an excellent soil improving effect even in a micro-soil, which has been difficult to treat in the conventional salt decontamination method, by using a mixed carrier of inorganic and organic.

In addition, the microbial soil amendment agent according to the present invention can supply beneficial microorganisms to the soil, can quickly regenerate the microorganisms and regenerate the soil without adversely affecting the soil and environment, thereby improving the productivity of vegetables and horticultural crops have.

In addition, the use of the microbial soil conditioner according to the present invention can minimize the number of times of soil improvement such as fresh water treatment and new soil change, so that the cultivated soil can be continuously used.

Hereinafter, the present invention will be described in detail.

The term " microbial soil conditioner "as used herein means a soil conditioner containing microorganisms.

The microbial soil conditioner for improving the crop growing soil of the present invention is a microbial soil preparation comprising 5 to 15% by weight of carbon source, 2 to 10% by weight of nitrogen source, 0.5 to 3% by weight of organic acid, 20 to 30% by weight of microbial culture solution, ; And a carrier to which the microorganism contained in the microorganism preparation is immobilized.

Soil modifiers containing microorganisms generally decrease their effectiveness over time. There is still a shortage of manufacturing techniques that can increase the effectiveness of microbial soil amendments or sustain and preserve their effects for a long time. It is difficult to guarantee the shelf life of the microorganism-containing soil preparation improver at a high concentration of 10 ^{7 to 9} cfu / g or more when storing the microorganism-containing preparation prepared in the liquid phase. There is a problem that the change greatly occurs.

In order to solve such a problem, the microbial soil amendment agent according to the present invention includes an inorganic-based carrier to which microorganisms are immobilized. When the microorganism-containing soil improving agent is formulated into a granular form using a porous inorganic material, the activity guarantee period becomes much longer than that in the case of a liquid phase.

The components of the microbial soil conditioner according to the present invention will be described below.

First, the microorganism preparation comprises 5 to 15% by weight of carbon source, 2 to 10% by weight of nitrogen source, 0.5 to 3% by weight of organic acid, 20 to 30% by weight of microorganism culture solution and remaining water. These microbial agents adsorb excess salts of the soil, supply effective microorganisms to the soil, and enhance the vitality of the soil microorganisms. In particular, when the soil is acidic with excess fertilization, it becomes insoluble easily and improves the vitality of the phosphoric acid-solubilizing bacteria which can not be absorbed well and enables the plants to use the accumulated phosphoric acid.

The carbon source is a necessary ingredient for the growth of microorganisms, and can be used quickly to enhance the vitality of the microorganism. As the carbon source, saccharides such as monosaccharides and disaccharides can be used, and molasses, dextrin, or a mixture thereof can be preferably used.

Molasses is a viscous liquid substance generated as a byproduct in the production of sugar, and the microbial vitality is rapidly increased during the microbial mixing treatment, which is highly effective.

Dextrin is a substance produced when starch is decomposed by reaction with water by the action of an acid such as hydrochloric acid, sulfuric acid, or an amylase, and when the dextrin is further hydrolyzed, it becomes a sugar or glucose. The dextrin is well soluble in cold water, and the thick aqueous solution is strong in tenacity, and in the microbial formulation according to one embodiment of the present invention, the dextrin can also act as a thickener.

Nitrogen sources also make microorganisms available quickly and increase the number of microorganisms. As the nitrogen source, amino acids, proteins, peptides and the like can be used, and whey protein, soybean protein, or a mixture thereof can be preferably used. Whey protein contains a large amount of amino acids, especially in the soil microbial lactic acid bacteria increases the vitality. Soybean protein is very effective in promoting vitality of Bacillus subtilis.

Organic acids regulate the pH of soils acidified by excessive fertilization and convert them into cropable soil. It also inhibits the proliferation of spoilage bacteria and chemically binds salinity, thereby eliminating elements that hinder plant growth. The organic acid may be at least one selected from the group consisting of acetic acid, citric acid, lactic acid, and humic acid.

The microorganism culture solution may be one in which Bacillus subtilis, Lactobacillus acidophilus, Saccharomyces cerevisiae, Photosynthetic bacteria or a mixture of at least one selected from these are cultured and concentrated. Bacillus, preferably promoting soil entry; A microorganism selected from the group consisting of lactic acid bacteria, yeast bacteria, and photosynthetic bacteria may be mixed, cultured, and concentrated. Lactic acid bacteria, yeast bacteria, and photosynthetic bacteria neutralize excess salt and allow the nutrients to be solubilized for use by plants, so it is more preferable to mix them with Bacillus subtilis.

The microbial culture preferably has a microbial content of 1.0 x 10 < ⁸ > cfu / ml or more.

Next, the carrier for improving the soil transferring power of the microbial agent will be described.

In general, rice bran is widely used as carrier and nutrient of microbial agent. Rice cores contain many nutrients in addition to carbohydrates and proteins, and when incorporated into the soil, they become a source of nutrients necessary for the growth of crops by the degradation of microorganisms. However, when rice bran is used alone as a microorganism carrier, the microbial carrier is rapidly decomposed by the microorganism when added to the soil, and the viability of the microorganism can not be sustained for a long time. Therefore, the present invention can prevent sudden changes in the physical properties of the carrier during soil supply and stably maintain the carrier performance and the viability of the microorganism by using a mixture of rice bran, main anchovy and calcium silicate as a carrier.

Liquor residue is residual grain after alcohol production and contains nutrients as shown in Table 1 including yeast fermentation by-products. Especially high protein content, contains various minerals. In the present invention, the microorganism can be used as a constituent component of the carrier, thereby enhancing the microbial growth.

Calcium silicate is a molded article having a tobermorite structure and has a particle size of 0.15 to 0.5 mm. The calcium silicate has a porous structure, which can enhance the soil physical properties such as drainage, air permeability and water retention, as well as carrying the carrier function. Therefore, the use of calcium silicate as a carrier can promote plant growth by increasing the propagation and activity of effective microorganisms. In addition, calcium silicate is a neutral calcium fertilizer which can supply not only calcium but also silicate, without increasing the pH of the soil, thereby contributing to promotion of growth of crops, improvement of quality and increase of production, have. In addition, calcium promotes the incorporation of soil and combines with corrosive acids to conserve soil organic matter. For example, nitrogen oxalate can be prevented to prevent the loss of nitrogen fertilizer.

The carrier according to the present invention is mixed with an organic material and an inorganic material, and the composition ratio of the organic material and the inorganic material is preferably about 60 to 70:30 to 40. When the organic matter is decreased, the rate of soil activation is decreased, and when the organic matter is increased, the durability of the carrier is decreased. If the mineral is used in excess of 40%, the soil conditioner may harden hard. The carrier may be composed of 30 to 40% by weight of calcium silicate, 50 to 68% by weight of rice bran, and 2 to 10% by weight of main anchovy.

The microbial soil amendment agent according to the present invention may be loaded on the carrier in an amount of 8 to 20% by weight based on the total weight of the microbial formulation.

It is to be understood, however, that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims, Will be apparent to those of ordinary skill in the art.

Example 1: Preparation of Microbial Soil Conditioner

10 g of whey protein (Sinwon Trading Co., USA), 10 g of soybean protein (Dongwoo Co., China), 5 g of acetic acid (Samseon powder, Korea) 250 ml of a microbial culture solution (KB Bio, Korea) consisting of Bacillus subtilis (1.4 × 10 ⁸ cfu / ml) and Lactobacillus acidophilus (1.2 × 10 ⁸ cfu / ml) was uniformly mixed and then the rest was filled with distilled water To prepare a liquid microorganism preparation.

The microbial agent was mixed in a ratio of 10% by weight to a carrier mixed with calcium silicate, rice bran and main anchors in a ratio of 35: 60: 5, respectively, to prepare a microbial soil conditioner.

Example 2: Application of microbial soil amendment agent

1) Forming process

- Test area size: 1m width × 5 × 3 length (block)

2) Applied crops: Chinese cabbage

3) Contents of processing

The soil fertilizer was supplied with the fertilizer as a standard by applying the cultivation fertilizer standard of the cabbage facility. After 3 days from the supply of the fertilizer, the microbial soil conditioner prepared in Example 1 was not treated (Comparative Example), 10 kg / 10a Example 2-1) and 20 kg / 10a (Example 2-2).

Experimental Example 1: Change in soil chemical composition

The physicochemical composition of the soil after the treatment with the microbial soil amendment agent was measured according to Example 2, and the results are shown in Table 3 below.

According to Table 3 above, Examples 2-1 and 2-2 treated with the microbial soil conditioner had a pH correction effect compared to the original soil before the pretreatment and the comparative example, and the EC was considerably increased. The decrease in the available phosphorus (Av.P ₂ O ₅ ) content in nutrients was judged to be due to the fact that phosphoric acid in the soil was absorbed by the plant. On the other hand, it is considered that the abundance of the substitutional cations, Ca, Mg, and K, is higher than that before the treatment, because the integrated slow - release nutrients were decomposed in the soil. It can be said that fertilizer integrated in the soil is efficiently used because it is decomposed and produced by microorganisms.

Experimental Example 2: Growth effect

The microbial soil conditioner was treated according to Example 2, and after 30 days, the cabbage growth was measured.

According to the results in Table 4, growth of leaf length, leaf width, and leaf number increased as the throughput of microbial soil conditioner increased. The increase of all growth factors compared to the comparative examples is believed to be due to the growth of the undergrowth due to the photosynthesis and the physiological mechanisms and the increase of the live weight.

Claims (9)

A microorganism preparation comprising 5 to 15% by weight of a carbon source, 2 to 10% by weight of a nitrogen source, 0.5 to 3% by weight of an organic acid, 20 to 30% by weight of a microbial culture solution, And
A carrier on which the microorganisms contained in the microorganism preparation are immobilized,
Wherein the carrier is composed of 30 to 40% by weight of calcium silicate, 50 to 68% by weight of rice bran, and 2 to 10% by weight of main anchovy.
The method according to claim 1,
Wherein the carrier comprises an organic material and an inorganic material in a weight ratio of 60 to 70: 30 to 40, and a microbial soil conditioner for improving crop cultivation soil.
delete The method according to claim 1,
Wherein the microbial agent is supported on a carrier in an amount of 8 to 20% by weight based on the total weight of the microbial soil conditioner.
The method according to claim 1,
Wherein the carbon source is molasses, dextrin, or a mixture thereof.
The method according to claim 1,
Wherein the nitrogen source is whey protein, soybean protein, or a mixture thereof.
The method according to claim 1,
Wherein the organic acid is at least one selected from the group consisting of acetic acid, citric acid, lactic acid, and humic acid.
The method according to claim 1,
The microorganism culture solution may be cultured in a culture medium containing at least one selected from the group consisting of Bacillus subtilis, Lactobacillus acidophilus, Saccharomyces cerevisiae, and photosynthetic bacteria. A microbial soil conditioner for soil improvement of cultivated crops.
The method according to claim 1,
The microbial culture solution may include Bacillus subtilis; And
Wherein the plant is cultivated by mixing at least one member selected from the group consisting of Lactobacillus acidophilus, Saccharomyces cerevisiae and Photosynthetic bacteria. Microbial soil conditioner.