CN111410290A - Nitrification construction and water body reuse method for biological floc aquaculture denitrification wastewater - Google Patents
Nitrification construction and water body reuse method for biological floc aquaculture denitrification wastewater Download PDFInfo
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- CN111410290A CN111410290A CN201910550929.4A CN201910550929A CN111410290A CN 111410290 A CN111410290 A CN 111410290A CN 201910550929 A CN201910550929 A CN 201910550929A CN 111410290 A CN111410290 A CN 111410290A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
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- C02F2101/16—Nitrogen compounds, e.g. ammonia
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Abstract
The invention belongs to the field of aquaculture, and particularly relates to a nitrification construction and water body reuse method of biological floc aquaculture denitrification wastewater, which comprises the steps of (1) nitrification construction, wherein the biological floc aquaculture wastewater subjected to denitrification treatment is kept in a stirred turbid state under the condition of dissolved oxygen concentration of more than 3 mg/L until the ammonia nitrogen concentration and the nitrite nitrogen concentration in the water body are both reduced to be less than 0.1 mg/L, so that nitrification construction is completed, (2) natural precipitation, namely, the water body subjected to nitrification construction is kept stand, so that the biological floc is naturally precipitated, and (3) water body transfer and aquaculture are carried out, wherein aquatic animals are stocked under the condition of dissolved oxygen concentration of more than 5 mg/L after the water body is separated from the biological floc precipitate.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a nitrification construction and water body reuse method of biological floc aquaculture denitrification wastewater.
Background
Environmental issues and limited natural resources place higher demands on aquaculture and water treatment technologies. In an indoor intensive aquaculture system without intense illumination, ammonia nitrogen generated in the culture process is mainly converted through two ways: 1) the microorganisms convert ammonia nitrogen into self biomass and organic nitrogen through assimilation; 2) nitrifying bacteria finally oxidize ammonia nitrogen into nitrate nitrogen through autotrophic nitrification. Research has shown that intensive aquaculture using biofloc technology (BFT) is still subject to nitrification, even under conditions where large additions of carbon source and heterotrophic assimilation predominate. Nitrifying BFT culture systems with little or no additional carbon source addition are continually reported and applied in order to fully utilize autotrophic nitrification in BFT culture systems.
In a nitrification type BFT culture system, the concentration of nitrate nitrogen is continuously and rapidly increased in the culture process; after the culture is finished, the heterotrophic denitrification treatment can be carried out on the culture wastewater by providing an anaerobic environment and adding a carbon source.
Research shows that in the biological floc culture wastewater after denitrification treatment, the relative abundance of nitrifying bacteria and the copy number of related functional genes are reduced to an extremely low level, namely the nitrification of a water body is insufficient. The denitrified biological floccule culture wastewater still contains other environmental harmful substances with certain concentration although the nitrate nitrogen concentration is lower, and if the wastewater is discharged into the external environment, the wastewater can cause harm to environmental protection and is a waste of water resources.
In order to realize the treatment and the repeated use of aquaculture wastewater, the prior art mainly treats the aquaculture wastewater through various bioreactors and water treatment equipment, and the water is treated and then pumped back to an aquaculture pond, so that a common circulating water aquaculture system is formed, and the repeated use of aquaculture water is realized. The technology and the types of the reactors are mature and rich, for example, a process flow chart of a pilot-scale system of closed cycle aquaculture recorded in the text of oxygenation effect of a gas/liquid mixing device in the closed cycle aquaculture system (journal of agricultural engineering, 2014, 30(06): 147-. However, the water treatment bioreactor, the water treatment equipment and the aquaculture water reuse process method relate to complex equipment types, high energy consumption and high management technical requirements, and the cost for constructing a set of water reuse recirculating aquaculture system is high.
Based on the technical analysis, if the water treatment function based on nitrification can be realized again on the denitrified biological floccule culture wastewater, the water body can be reused as a nitrification BFT culture system, the reuse of culture water is realized among a plurality of aquaculture periods, and the environmental protection and the water resource saving are facilitated.
Disclosure of Invention
Aiming at the denitrification-treated biological floc aquaculture wastewater, the invention provides a nitrification construction and water body reuse method of the biological floc aquaculture denitrification wastewater, which realizes the reuse of aquaculture water, environmental protection and water resource saving. The method does not need a special biological denitrification reactor, has low requirements on equipment and environment, is simple and convenient to operate, is easy to control and realize, does not need special pretreatment and/or early culture, reduces the cost and improves the benefit.
The invention adopts the technical scheme that a nitrification construction and water body reuse method of biological floc aquaculture denitrification wastewater comprises the steps of (1) nitrification construction, wherein the biological floc aquaculture wastewater subjected to denitrification treatment is kept in a stirred turbid state under the condition of dissolved oxygen concentration of more than 3 mg/L until the ammonia nitrogen concentration and the nitrite nitrogen concentration in the water body are both reduced to be less than 0.1 mg/L, so that nitrification construction is completed, (2) natural precipitation, namely, the water body subjected to nitrification construction is kept stand, so that the biological floc is naturally precipitated, and (3) water body transfer and culture, wherein aquatic animals are released under the condition of dissolved oxygen concentration of more than 5 mg/L after the water body is separated from the biological floc precipitate.
After denitrification treatment is finished, the biological floc culture wastewater for aquatic animal culture can be used for aquatic animal culture again after nitrification construction, natural precipitation and water body transfer, and the repeated recycling of the aquatic animal culture water body is realized.
In the step (1), the temperature of nitrification construction water is 20-33 ℃.
Step (1), the aeration rate of the aeration equipment per cubic meter of water body is 0.16m3More than h, preferably the aeration rate per cubic meter of water body is 0.4m3The concentration of dissolved oxygen is preferably 4 to 6 mg/L, and the concentration of dissolved oxygen is maintained at 3 mg/L or higher.
And (1) through nitrification construction, the concentration of ammonia nitrogen in the water body is reduced to be below 0.08 mg/L, and the concentration of nitrite nitrogen in the water body is reduced to be below 0.05 mg/L.
And (1) keeping a turbid state under the action of an electric stirrer with the rotating speed of 200-400 rpm.
And (1) establishing nitrification for 8-12 days, preferably 10 days.
And (2) standing the water body constructed by the nitrification for 1.5-3 hours, preferably 2 hours.
And (2) standing the water body constructed by the nitrification, naturally precipitating the biological flocs until the total suspended particulate matter concentration (TSS) in the water body is reduced to 50-150 mg/L, and obtaining the nitrified BFT culture water body with low biological floc concentration suitable for aquatic animal culture.
Step (3), the aeration rate of the aeration equipment per cubic meter of water body is 0.66m3More than h, preferably 2m of aeration per cubic meter of water body3At least one hour, so as to maintain a dissolved oxygen concentration of 5 mg/L or more, the dissolved oxygen concentration is 5 to 9 mg/L, preferably 5 to 9 mg/L7.5mg/L。
And (3) transferring the water body into another container by adopting power devices such as a water pump and the like to realize the precipitation separation of the water body and the biological flocs. The container comprises a culture pond or a container with a similar liquid containing function to the culture pond, such as a barrel, a tank or a groove.
As a water quality control technology for the suspension growth of microorganisms, the Biological Floc Technology (BFT) has the advantage of small conflict between autotrophic nitrification and heterotrophic denitrification treatment, and can realize nitrification in a water body more easily. Through the technical treatment of the invention, the water treatment function based on nitrification can be realized again on the denitrified biological floccule culture wastewater, and the denitrified wastewater can be reused as a nitrifying BFT culture system, so that the water-treated microorganisms and aquaculture animals grow in the same space, the reuse of culture water is simply realized among a plurality of aquaculture cycles, and the invention is beneficial to environmental protection and water resource saving.
Compared with the prior art, the invention has the advantages that no special water treatment equipment is used, only a simple container similar to an aquaculture pond is used, the retreatment of the denitrified wastewater can be completed under the aeration condition, the water body becomes a nitrifying BFT culture system again and is used for aquaculture again, and after the treatment by the technology of the invention, main toxic substances (ammonia nitrogen and nitrite nitrogen) harmful to the aquatic animals in the water body can be continuously controlled to be at a low concentration level below 0.1 mg/L.
Drawings
FIG. 1 is a flow chart of nitrification construction and water reuse process of denitrification wastewater of biological floc aquaculture in example 1.
FIG. 2 is a graph showing the trend of the change in the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the nitrification construction process in example 1.
FIG. 3 is a graph showing the trend of the change of the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the water during the tilapia culture process in example 1.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1
The method comprises the steps of performing tilapia freshwater aquaculture for 116 days by using a nitrification type biological floc technology, obtaining initial aquaculture wastewater after harvesting tilapia, adding 1.5kg of monohydrate glucose into each cubic meter of wastewater, performing denitrification treatment for 8 days, and obtaining denitrified biological floc aquaculture wastewater, wherein the denitrified wastewater is characterized by comprising 103.2 mg/L of total nitrogen, 4.5 mg/L of nitrate nitrogen, 0.02 m/L of nitrite nitrogen and 5.4 mg/L of ammonia nitrogen.
Then sequentially carrying out nitrification construction and reuse on the denitrified biological floccule aquaculture wastewater according to the steps of nitrification construction, natural sedimentation, water body transfer and aquaculture shown in figure 1:
(1) construction of nitrification: using three circular water buckets with the inner diameter of 0.45m, and injecting 60 liters of denitrified biological floccule aquaculture wastewater into each circular water bucket; each round bucket is provided with an electric stirrer (the rotating speed is 240rpm), an aeration stone and a corresponding air supply pipeline, and the air flow rate for aeration of each round bucket (60 liters of water) is 0.01m3The method comprises the steps of firstly, aerating and stirring the water body for 10 days, wherein the average dissolved oxygen concentration of the water body is 4.6 mg/L, then, sequentially increasing the ammonia nitrogen concentration and the nitrite nitrogen concentration in the water body, and then, with the continuous increase of nitrate nitrogen, sequentially and respectively reducing the ammonia nitrogen concentration and the nitrite nitrogen concentration to 0.08 mg/L and 0.05 mg/L, thus completing the construction of nitrification, wherein in the construction process of nitrification, the respective concentration change trend graphs of the ammonia nitrogen, the nitrite nitrogen and the nitrate nitrogen are shown in figure 2.
(2) And (4) naturally precipitating, namely closing the aeration and stirring equipment, standing the water body after the nitrification is constructed, and precipitating for 2 hours until the TSS concentration of the upper water body is reduced to 101 mg/L, wherein the concentration is suitable for aquaculture of aquatic animals.
(3) Water body transfer: and transferring the upper water body after natural sedimentation for 2 hours to three other circular buckets with the inner diameter of 0.45m by using a water pump, thus obtaining the nitrified BFT culture water body with lower biological floc concentration.
(4) Aquaculture: each new round bucket is provided with an aeration stone and a corresponding air supply pipeline, and the aeration quantity of each round bucket (60 liters of water) is 0.04m3And then breeding 6 tails of tilapia in each round bucket, and feeding commercial compound feed according to 2 percent of the total weight of the tilapia every day for breeding, wherein the main water quality condition in the tilapia breeding process is shown in figure 3, and the tilapia growth condition is shown in figure 1.
TABLE 1 growth of Tilapia mossambica
| Initial individual average weight (g) | Average weight of end units (g) | Survival rate (%) | Coefficient of bait | Cultivation time (sky) |
| 45.1 | 49.7 | 100 | 1.4 | 7 |
Claims (4)
1. A nitrification construction and water body reuse method of biological floc aquaculture denitrification wastewater is characterized by comprising the following steps:
(1) nitrification construction, namely maintaining the stirred turbid state of the denitrified biological floc aquaculture wastewater under the condition of dissolved oxygen concentration of more than 3 mg/L until the ammonia nitrogen concentration and the nitrite nitrogen concentration in the water body are both reduced to be less than 0.1 mg/L, so that the nitrification construction is completed;
(2) and (3) natural precipitation: standing the water body which is constructed by the nitrification to ensure that the biological floccules are naturally precipitated;
(3) water transfer and culture, namely, after the water and the biological floccules are precipitated and separated, stocking aquatic animals under the condition of dissolved oxygen concentration of more than 5 mg/L.
2. The nitrification construction and water reuse method according to claim 1, wherein in step (1), the aeration amount of the aeration equipment provided per cubic meter of water is 0.16m3At least one hour, so as to maintain a dissolved oxygen concentration of 3 mg/L or higher.
3. The nitrification construction and water body reuse method according to claim 1, wherein in the step (2), the concentration of total suspended particulate matters naturally precipitated into the water body by the biological flocs is reduced to 50-150 mg/L.
4. The nitrification construction and water reuse method according to claim 1, wherein, in step (3), the aeration rate of the aeration equipment provided per cubic meter of water is 0.66m3At least one hour, so as to maintain a dissolved oxygen concentration of at least 5 mg/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115072883A (en) * | 2022-06-17 | 2022-09-20 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130256223A1 (en) * | 2010-12-02 | 2013-10-03 | Guanghao Chen | Biological wastewater treatment and reuse utilizing sulfur compounds as electron carrier to minimize sludge production |
| CN106754552A (en) * | 2017-01-20 | 2017-05-31 | 国家海洋局第三海洋研究所 | A kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130256223A1 (en) * | 2010-12-02 | 2013-10-03 | Guanghao Chen | Biological wastewater treatment and reuse utilizing sulfur compounds as electron carrier to minimize sludge production |
| CN106754552A (en) * | 2017-01-20 | 2017-05-31 | 国家海洋局第三海洋研究所 | A kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion |
Non-Patent Citations (1)
| Title |
|---|
| 刘文畅 等: "生物絮团水产养殖系统的水体后处理和重复使用研究", 《2018年中国水产学会学术年会论文摘要集》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115072883A (en) * | 2022-06-17 | 2022-09-20 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
| CN115072883B (en) * | 2022-06-17 | 2023-11-14 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
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Application publication date: 20200714 |