CN113651432A - Microbial biofilm carrier for removing ammonia nitrogen in water body and preparation method thereof - Google Patents
Microbial biofilm carrier for removing ammonia nitrogen in water body and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/084—Polymers containing vinyl alcohol units
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention relates to a microbial biofilm carrier for removing ammonia nitrogen from a polluted water body and a preparation method thereof, belonging to the technical field of microbial biofilm carriers, wherein the microbial biofilm carrier is obtained by rapidly screening and culturing the polluted water body to obtain indigenous ammonia nitrogen for degerming, and then combining the obtained indigenous ammonia nitrogen for degerming with a hydroformylation vinylon carrier; according to the invention, indigenous ammonia nitrogen is rapidly screened from the polluted water body for degerming, and a fixed biological membrane is prepared and put into the target water body, so that the problem of ammonia nitrogen bacteria removal adaptability is solved, and meanwhile, the target microorganism is cultured and attached to the corresponding carrier to prepare the fixed biological membrane hung on the surface of the carrier, so that the loss of the target bacteria is reduced and the removal efficiency of the ammonia nitrogen in the water body is improved.
Description
Technical Field
The invention belongs to the technical field of microbial biofilm carriers, and particularly relates to a microbial biofilm carrier for removing ammonia nitrogen in a polluted water body and a preparation method thereof.
Background
Ammonia nitrogen is always used as an important index parameter for water body treatment and drainage in sewage treatment and river treatment, and is free ammonia (NH)3) Or ammonium ion (NH)4 +) The form exists. The ammonia nitrogen has certain harm effect on aquatic organisms, the excessive ammonia nitrogen can also cause the increase of the nitrite content in the water body, and when the nitrite in the water is too high, the nitrite can be combined with protein to form a strong carcinogenic substance nitrosamine. In addition, the harm of ammonia nitrogen to aquatic animals can cause slow growth of fishes and the like, loss of balance in water and even death of serious people. The growth of the algae in the water body preferentially utilizes ammonia nitrogen as a nitrogen source, the excessive ammonia nitrogen causes the algae to be inundated, and the ammonia nitrogen can be increased rapidly after the algae die, so that the deterioration of the water body is aggravated.
The ammonia nitrogen in the polluted water is mainly from the decomposition of nitrogenous organic matters in domestic sewage and environmental pollutants, and can be finally biologically oxidized into nitrate stable chemical substances under the action of microorganisms in the water environment or a sewage treatment system, so that the self-purification or standard-reaching treatment of the river is completed. When the ammonia nitrogen content in the water body exceeds the self-purification or treatment capacity of the polluted water body, human intervention and adjustment are needed. At present, aiming at the problem that ammonia nitrogen in a polluted water body exceeds the standard, besides increasing dissolved oxygen in the water body, the ammonia nitrogen removing microbial inoculum put in by an artificial person is also widely applied. Most of the ammonia nitrogen removal microbial inoculum sold on the market is a mixed preparation product of one or more fixed bacteria, but the conditions such as trace elements, a carbon source (BOD), pH, salinity and the like of different water bodies are different, and exogenous microorganisms can not adapt to a target water body necessarily, so that the released ammonia nitrogen removal microorganisms die slowly, and the long-acting treatment target is difficult to achieve. Therefore, the microorganism biofilm carrier for removing the ammonia nitrogen in the polluted water body and the preparation method thereof are provided.
Disclosure of Invention
In order to solve the problem of insufficient adaptability of exogenous microorganism bacteria to the target water environment, the invention provides a microorganism biofilm carrier for removing ammonia nitrogen in water and a preparation method thereof. The method has the advantages of easy operation, strong pertinence and simple implementation condition requirement, and can be applied to the fields of black and odorous water body treatment, sewage treatment and the like.
The invention realizes the purpose through the following technical scheme:
a preparation method of a microbial biofilm carrier for removing ammonia nitrogen in a water body comprises the steps of quickly screening and culturing a polluted water body to obtain indigenous ammonia nitrogen for degerming, and then combining the obtained indigenous ammonia nitrogen for degerming with a hydroformylation vinylon carrier to obtain the microbial biofilm carrier.
As a further optimization scheme of the invention, the specific steps of rapidly screening and culturing the polluted water body to obtain the indigenous ammonia nitrogen for degerming are as follows:
(1) sampling of the polluted water body: selecting 3-4 positions in the range of the target polluted water body as sampling points, taking water samples containing mud on the surface layer and the bottom layer of the water body, mixing the water samples on the surface layer and the water samples on the bottom layer, and storing at room temperature to obtain 3-4 independent mixed water samples;
(2) screening and enriching for removing bacteria of indigenous ammonia nitrogen: adding a solid screening enrichment medium into each mixed water sample, stirring and dissolving, testing the initial ammonia nitrogen value of the water sample, and carrying out aeration culture;
(3) and (3) detecting the ammonia nitrogen removal performance of the strain: after the aeration culture is finished, detecting ammonia nitrogen values in all groups of screening enrichment cultures, comparing the ammonia nitrogen values with original ammonia nitrogen values, and selecting the screening enrichment cultures with the highest ammonia nitrogen removal rate as target indigenous ammonia nitrogen for degerming;
(4) amplification culture: and (3) mixing the native ammonia nitrogen degerming obtained in the step (2) and the step (3) with target sewage according to the proportion of 1-5: 100, adding a solid screening enrichment medium, and carrying out aeration culture to obtain a large amount of native ammonia nitrogen degerming.
As a further optimization scheme of the invention, the specific steps of the combination of the removal of bacteria from the indigenous ammonia nitrogen and the hydroformylation vinylon fiber carrier are as follows:
and (3) introducing the obtained native ammonia nitrogen degerming bacteria and target sewage into a reaction tank according to the ratio of 1: 5-10, adding an ammonia nitrogen removing bacteria nutrient medium, adding a hydroformylation vinylon as an ammonia nitrogen removing bacteria biofilm carrier into the reaction tank, carrying out aeration culture for 2-3 days until a light yellow slimy bacteria biofilm is attached to the carrier, and thus completing preparation of the biofilm carrier.
As a further optimization scheme of the invention, the culture medium comprises the following components: 3-6 parts of sodium citrate and 0.3-0.6 part of (NH)4)2SO40.4-0.8 parts of composite trace salt.
As a further optimization scheme of the invention, the compound trace salt consists of the following substances: 4-6 parts by weight of K2HPO40.04 to 0.06 portion of FeSO4·7H2O, 2-3 parts of NaCl, 2-3 parts of MgSO4·7H2O, 0.04-0.06 parts of MnSO4·4H2O。
As a further optimization scheme of the invention, the solid screening enrichment medium further comprises 0.3-0.6 part of NH by weight4Cl。
As a further optimized scheme of the invention, the xanthan gum further comprises 2-4 parts of water-soluble starch and 0.1-0.5 part of xanthan gum by weight.
As a further optimization scheme of the invention, the hydroformylation vinylon yarn carrier consists of a PP central rope and hydroformylation vinylon yarns, wherein each PP central rope is 2-3 meters long and has a diameter of 1-2 mm, a bundle of hydroformylation vinylon yarns is fixed on each central rope every 10-15 cm, and each bundle of hydroformylation vinylon yarns is 15-20 cm long and contains 150-200 yarns of hydroformylation vinylon yarns.
The invention also provides a microbial biofilm carrier for removing ammonia nitrogen in the water body, which is prepared by the preparation method of the microbial biofilm carrier for removing ammonia nitrogen in the water body.
The invention has the beneficial effects that: according to the invention, the method comprises the steps of screening, enriching and degerming the indigenous ammonia nitrogen from the polluted water body, preparing active bacteria liquid and combining the active bacteria liquid with the hydroformylation vinylon carrier, fixing the screened indigenous ammonia nitrogen degerming through the carrier, forming a functional microbial film on the carrier, fixing the film-forming carrier on the polluted water body, and fully contacting the polluted water body.
The method screens and enriches the native ammonia nitrogen from the polluted water body to remove bacteria by selectively screening and enriching the culture medium, and the culture medium has good screening characteristics and enrichment nutrition characteristics, and is simple to operate and strong in environmental adaptability. Additional selection of enrichment Medium Using excess NH4And Cl, increasing the concentration of ammonia nitrogen in the culture water body, monitoring the concentration by a nano reagent spectrophotometry, and evaluating the ammonia nitrogen removal efficiency as the screening effect judgment of the screened indigenous ammonia nitrogen removing bacteria. Xanthan gum is added into a nutrient medium used in the preparation process of the biofilm carrier as an adhesive to promote functional strains to better biofilm on the carrier. The obtained functional strain has high nitrification efficiency and strong adaptability, is functional bacteria existing in the polluted water, is applied to the treatment of the polluted water body, does not have secondary pollution, and enhances the self-purification capability of the polluted water body.
Drawings
FIG. 1 is a schematic diagram of the strain culture of a microbial biofilm carrier for removing ammonia nitrogen from a water body according to the invention;
FIG. 2 is a schematic diagram of a hydroformylation vinylon carrier of a microorganism biofilm formation carrier for removing ammonia nitrogen in a water body.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
In order to verify the effects of the present invention, the following specific examples are provided.
Example 1
The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in water in the embodiment is as follows:
(1) sampling of the polluted water body: and 4 positions are selected as sampling points in the range of the target polluted water body, wherein the sampling points are respectively positioned at two ends of the river channel and at the river channel terminal 2. Taking water samples containing mud on the surface layer and the bottom layer of the water body by using a 1L vacuum sampling bottle at each sampling point, then mixing the water samples on the surface layer and the bottom layer, putting the mixture into a 4-5L container, and storing at room temperature to obtain 3-4 independent mixed water samples; the target polluted water body in the embodiment is a new north-region exquisite river in Changzhou city, 4 positions are selected as sampling points within 4 kilometers of the exquisite river, wherein the sampling points are respectively positioned at two ends of a river channel and at a river channel terminal 2, the new north-region exquisite river in Changzhou city is an urban river and belongs to a river channel polluted water body, the river channel is in 4 types of water quality for a long time, and the ammonia nitrogen value of the water body exceeds the standard occasionally;
(2) screening and enriching for removing bacteria of indigenous ammonia nitrogen: adding a solid screening enrichment culture medium into each mixed water sample, adding 7g/L of the solid screening enrichment culture medium into each liter of the water sample, stirring and dissolving, testing the initial ammonia nitrogen value of the water sample by using a nano reagent spectrophotometry, carrying out aeration culture, and culturing at 20-35 ℃ for 48h, wherein the dissolved oxygen concentration is 2-4 mg/L;
(3) and (3) detecting the performance of the screened strains: after the culture is finished for 48h, detecting the ammonia nitrogen value in each group of screening enrichment culture by using a nano reagent spectrophotometry, comparing the ammonia nitrogen value with the original ammonia nitrogen value, and selecting the culture group with the lowest ammonia nitrogen value as the final indigenous ammonia nitrogen for degerming;
(4) amplification culture: selecting a large-volume container, mixing the native ammonia nitrogen degerming obtained in the step (3) with target sewage (river water) according to the proportion of 5:100, adding a solid screening enrichment medium with the addition of 7g/L, carrying out aeration culture, carrying out culture at 20-35 ℃ for 48h, and obtaining a large amount of native ammonia nitrogen degerming, wherein the dissolved oxygen concentration is 2-4 mg/L;
(5) preparing a membrane-forming carrier: and (3) introducing a large amount of indigenous ammonia nitrogen degerming obtained in the step (4) and target sewage (river water) into a reaction tank according to the ratio of 1:10, simultaneously adding 10g/L of ammonia nitrogen degerming nutrient medium, and adding hydroformylation vinylon fibers as ammonia nitrogen removing bacteria biofilm carriers into the reaction tank, wherein the dosage of the carriers is 40% of the volume of the reaction tank as shown in figure 2, carrying out aeration culture for 2 days until the carriers are attached with faint yellow slimy bacteria biofilm carriers, and fixing the biofilm carriers into a target water body for removing ammonia nitrogen as shown in a right bottle in figure 1.
Wherein the screening enrichment medium in the step (2) consists of the following components: 6 parts of sodium citrate and 0.3 part of (NH)4)2SO40.4 parts of composite trace salt and 0.3 part of NH4Cl; the nutrient medium in the step (5) consists of the following components: 4 parts of water-soluble starch, 6 parts of sodium citrate and 0.4 part of (NH)4)2SO40.4 part of composite trace salt and 0.5 part of xanthan gum; the compound trace salt consists of the following components: based on the weight portion, 6 portions of K2HPO40.04 part of FeSO4·7H2O, 2 parts of NaCl, 2 parts of MgSO4·7H2O, 0.04 part of MnSO4·4H2O; the hydroformylation vinylon yarn carrier in the step (5) is composed of a PP material central rope with the length of 2 meters and the diameter of 2mm and hydroformylation vinylon yarns, wherein one bundle of hydroformylation vinylon yarns is fixed on the central rope every 15cm, and each bundle of hydroformylation vinylon yarns with the length of 15cm contains 200 yarns of hydroformylation vinylon yarns.
The film hanging carrier is composed of a PP material central rope and hydroformylation vinylon wires, each PP material central rope is 2-3 meters long and phi 1-2 mm long, a bundle of hydroformylation vinylon is fixed on the central rope every 10-15 cm, and each bundle of hydroformylation vinylon is 15-20 cm long and contains 150-200 silks of hydroformylation vinylon. In a 4-kilometer test river section of the exquisite river, the longitudinal and transverse spacing distances between the microbial biofilm carriers are 0.5-2m, and the detection indexes after treatment are as follows: after three months of treatment, peculiar smell is eliminated, black and odorous bottom mud is changed into grey white, green algae in water is basically eliminated, after 6 months, the water quality state is stable, ammonia nitrogen in water is reduced to 0.31mg/L from 7.85mg/L, total nitrogen in water is reduced to 0.54mg/L from 9.13mg/L, dissolved oxygen in water is increased to 6.2mg/L from 3.3mg/L, and the pH value is 6.5-8.0; sulfide is less than 0.2mg/L, and in six months of implementation, the water sample around the microorganism biofilm carrier shows effective viable bacteriaNumber average is more than or equal to 2 multiplied by 107The/g shows that the microbial biofilm carrier has strong adaptability in the water body and can carry out long-acting treatment on the water body.
For comparison, the commercial ammonia nitrogen removal nitrobacteria are used for preparing a microorganism biofilm carrier, the microorganism biofilm carrier is thrown into a exquisite river test river reach in the same throwing mode, the microorganism biofilm carrier shows that the number of effective viable bacteria is about 2 hundred million/mL in the early implementation stage, the number of the effective viable bacteria of a water sample around the microorganism biofilm carrier gradually decreases after the 2 nd month, and the number of the effective viable bacteria is less than 5 multiplied by 10 by the 6 th month4/g。
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (8)
1. A preparation method of a microbial biofilm carrier for removing ammonia nitrogen in a polluted water body is characterized by comprising the steps of quickly screening and culturing the polluted water body to obtain indigenous ammonia nitrogen for degerming, and then combining the obtained indigenous ammonia nitrogen for degerming with a hydroformylation vinylon fiber carrier to obtain the microbial biofilm carrier.
2. The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in water bodies according to claim 1, which is characterized in that the specific steps of rapidly screening and culturing the polluted water bodies to obtain the indigenous ammonia nitrogen for degerming are as follows:
(1) sampling of the polluted water body: selecting 3-4 positions in the range of the target polluted water body as sampling points, taking water samples containing mud on the surface layer and the bottom layer of the water body, mixing the water samples on the surface layer and the water samples on the bottom layer, and storing at room temperature to obtain 3-4 independent mixed water samples;
(2) screening and enriching for removing bacteria of indigenous ammonia nitrogen: adding a solid screening enrichment medium into each mixed water sample, stirring and dissolving, testing the initial ammonia nitrogen value of the water sample, and carrying out aeration culture;
(3) and (3) detecting the ammonia nitrogen removal performance of the strain: after the aeration culture is finished, detecting ammonia nitrogen values in all groups of screening enrichment cultures, comparing the ammonia nitrogen values with original ammonia nitrogen values, and selecting the screening enrichment cultures with the highest ammonia nitrogen removal rate as target indigenous ammonia nitrogen for degerming;
(4) amplification culture: and (3) mixing the native ammonia nitrogen degerming obtained in the step (2) and the step (3) with target sewage according to the proportion of 1-5: 100, adding a solid screening enrichment medium, and carrying out aeration culture to obtain a large amount of native ammonia nitrogen degerming.
3. The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in the water body according to claim 1, wherein the specific steps of removing bacteria of the indigenous ammonia nitrogen and combining the hydroformylation vinylon fiber carrier are as follows:
and (3) introducing the obtained native ammonia nitrogen degerming bacteria and target sewage into a reaction tank according to the ratio of 1: 5-10, adding an ammonia nitrogen removing bacteria nutrient medium, adding a hydroformylation vinylon as an ammonia nitrogen removing bacteria biofilm carrier into the reaction tank, carrying out aeration culture for 2-3 days until a light yellow slimy bacteria biofilm is attached to the carrier, and thus completing preparation of the biofilm carrier.
4. The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in water bodies according to any one of claims 2 or 3, wherein the enrichment medium comprises the following components: 3-6 parts of sodium citrate and 0.3-0.6 part of (NH)4)2SO40.4-0.8 parts of composite trace salt.
5. The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in water bodies according to claim 4, wherein the compound trace salt consists of the following components: 4-6 parts by weight of K2HPO40.04 to 0.06 portion of FeSO4·7H2O, 2-3 parts of NaCl, 2-3 parts of MgSO4·7H2O, 0.04-0.06 parts of MnSO4·4H2O。
6. The method for preparing a microorganism biofilm carrier for removing ammonia nitrogen in a water body according to claim 5, which is characterized in that the solid screening enrichment medium further comprises 0.3-0.6 part by weight of NH4Cl。
7. The preparation method of the microbial biofilm carrier for removing ammonia nitrogen in water bodies according to claim 5, wherein the ammonia nitrogen removing bacteria nutrient medium further comprises 2-4 parts by weight of water-soluble starch and 0.1-0.5 part by weight of xanthan gum.
8. A microorganism biofilm carrier for removing ammonia nitrogen in water is characterized by being prepared by the preparation method of the microorganism biofilm carrier for removing ammonia nitrogen in water according to any one of claims 1 to 3 and 5 to 7.
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2021
- 2021-09-01 CN CN202111020987.XA patent/CN113651432A/en active Pending
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| CN101343114A (en) * | 2008-08-16 | 2009-01-14 | 王光玉 | Biological denitrification method for wastewater of industrial cultivation |
| CN106676022A (en) * | 2015-11-06 | 2017-05-17 | 丹阳市尚德生物科技有限公司 | Method for preparing microbe bacteria liquid for treating black-odor riverway |
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