CN114561293A - Method for purifying cefradine wastewater based on microalgae culture - Google Patents
Method for purifying cefradine wastewater based on microalgae culture Download PDFInfo
- Publication number
- CN114561293A CN114561293A CN202111600388.5A CN202111600388A CN114561293A CN 114561293 A CN114561293 A CN 114561293A CN 202111600388 A CN202111600388 A CN 202111600388A CN 114561293 A CN114561293 A CN 114561293A
- Authority
- CN
- China
- Prior art keywords
- microalgae
- wastewater
- purifying
- cefradine
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002351 wastewater Substances 0.000 title claims abstract description 35
- 229960002588 cefradine Drugs 0.000 title claims abstract description 34
- RDLPVSKMFDYCOR-UEKVPHQBSA-N cephradine Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CCC=CC1 RDLPVSKMFDYCOR-UEKVPHQBSA-N 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000003115 biocidal effect Effects 0.000 claims abstract description 16
- 230000003698 anagen phase Effects 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 23
- 241000195661 Scenedesmus quadricauda Species 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 6
- 239000002028 Biomass Substances 0.000 abstract description 9
- 108010052832 Cytochromes Proteins 0.000 abstract description 2
- 102000018832 Cytochromes Human genes 0.000 abstract description 2
- 239000003344 environmental pollutant Substances 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 230000029553 photosynthesis Effects 0.000 abstract description 2
- 238000010672 photosynthesis Methods 0.000 abstract description 2
- 231100000719 pollutant Toxicity 0.000 abstract description 2
- 150000003839 salts Chemical class 0.000 abstract description 2
- 238000004065 wastewater treatment Methods 0.000 abstract description 2
- ZZORFUFYDOWNEF-UHFFFAOYSA-N sulfadimethoxine Chemical compound COC1=NC(OC)=CC(NS(=O)(=O)C=2C=CC(N)=CC=2)=N1 ZZORFUFYDOWNEF-UHFFFAOYSA-N 0.000 description 26
- 229960000973 sulfadimethoxine Drugs 0.000 description 26
- 241000195663 Scenedesmus Species 0.000 description 16
- 239000003242 anti bacterial agent Substances 0.000 description 15
- 229940088710 antibiotic agent Drugs 0.000 description 15
- 241000195493 Cryptophyta Species 0.000 description 11
- 235000021466 carotenoid Nutrition 0.000 description 11
- 150000001747 carotenoids Chemical class 0.000 description 11
- 229930002875 chlorophyll Natural products 0.000 description 10
- 235000019804 chlorophyll Nutrition 0.000 description 10
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 229930186147 Cephalosporin Natural products 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 229940124587 cephalosporin Drugs 0.000 description 5
- 150000001780 cephalosporins Chemical class 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 150000002960 penicillins Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to the field of wastewater treatment, and particularly relates to a method for purifying cefradine wastewater based on microalgae culture, which comprises the following steps: 1) pre-culturing functional microalgae to reach logarithmic growth phase; 2) and inoculating the functional microalgae in the logarithmic growth phase in the step 1) into the wastewater containing the cefradine antibiotic to purify the antibiotic wastewater. The invention purifies the high-concentration antibiotic wastewater by utilizing the microalgae, does not need to add nutrient salt, and purifies pollutants by utilizing the photosynthesis of the microalgae to obtain the biomass of the microalgae and the cytochrome.
Description
Technical Field
The invention belongs to the field of wastewater treatment, and particularly relates to a method for purifying cefradine wastewater based on microalgae culture.
Background
The cephalosporin has high efficiency, low toxicity and wide clinical application, has the advantages of wide antibacterial spectrum, strong antibacterial effect, penicillinase resistance, rare allergic reaction compared with penicillins and the like, can not only destroy the cell wall of bacteria, but also sterilize in the propagation period of the bacteria, and has almost no toxicity to organisms, thereby being widely applied clinically.
The waste water from the production of cephalosporin antibiotics often contains a large amount of organic substances and components of cephalosporin antibiotics. Since cephalosporin antibiotics are broad-spectrum antibiotics, they destroy the cell wall of bacteria, rendering the bacteria inactive. High-concentration cephalosporin antibiotic wastewater is difficult to effectively treat by using a traditional activated sludge method, because bacteria are target cells of the antibiotics, the growth of the bacteria can be influenced by the existence of the antibiotics, and the effect of purifying the wastewater by the bacteria is influenced. In addition, the proliferation of the microorganism under the condition of high antibiotic concentration can be induced to generate antibiotic resistance genes, and the microorganism carrying the genes can reduce the antibiotic effect and generate great harm if entering the environment.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for purifying cefradine wastewater based on microalgae culture.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for purifying cefradine wastewater based on microalgae culture comprises the following steps:
1) pre-culturing functional microalgae to reach logarithmic growth phase;
2) and inoculating the functional microalgae in the logarithmic growth phase in the step 1) into the wastewater containing the cefradine antibiotic to purify the antibiotic wastewater.
The functional microalgae is Scenedesmus quadricauda or Chlorella L166.
The concentration of the cefradine in the step 2) is 25-100 mg/L.
The conditions of the pre-culture were set as: illumination all day long, illumination intensity 4800-2And N2Mixed gas of (2), CO2The occupied volume fraction is 5%.
Compared with the prior art, the invention has the beneficial effects that:
1) the invention utilizes microalgae culture to purify artificial simulation high-concentration antibiotic wastewater, and the degradation rate of Scenedesmus quadricauda to antibiotics is more than 95% after 8 days of culture. Meanwhile, the biomass concentration of Scenedesmus quadricaudatus after 8 days can reach 111.85 +/-1.26 mg/L, the chlorophyll content reaches 20.00 +/-1.58 mg/g, and the carotenoid content reaches 7.36 +/-0.26 mg/g. After 8 days of culture, the degradation rate of chlorella L166 to antibiotics is above 85%. Meanwhile, the biomass concentration of the chlorella L1668 days can reach 138.19 +/-0.37 mg/L, the chlorophyll content reaches 18.78 +/-0.38 mg/g, and the carotenoid content reaches 6.80 +/-0.03 mg/g.
2) The invention purifies the high-concentration antibiotic wastewater by utilizing the microalgae, does not need to add nutrient salt, and purifies pollutants by utilizing the photosynthesis of the microalgae to obtain the biomass of the microalgae and the cytochrome.
3) Carotenoids produced by the microalgae can eliminate active oxygen such as triplet chlorophyll and singlet oxygen produced by the microalgae under the stress of antibiotics, and are beneficial to purification of the antibiotics. In the method, the carotenoid content produced by the microalgae under the stress of the antibiotics is obviously higher than that produced by the microalgae without the stress of the antibiotics. It is shown that this alga is very suitable for the elimination of antibiotics and can achieve a very high purification efficacy.
Drawings
FIG. 1 is a graph showing the results of the degradation rate of Scenedesmus quadricauda in example 1 to different concentrations of cephradine;
FIG. 2 is a graph showing the change in biomass concentration of Scenedesmus quadricaudatus at different concentrations of cephradine in example 1;
FIG. 3 is a graph showing the change in chlorophyll content of Scenedesmus quadricauda at different concentrations of cephradine in example 1;
FIG. 4 is a graph showing the change in carotenoid content of Scenedesmus quadricaudatus at different concentrations of cefradine in example 1.
FIG. 5 is a graph showing the results of the degradation rate of the chlorella L166 to cephradine in different concentrations in example 2;
FIG. 6 is a graph showing the change in biomass concentration of Chlorella L166 under different concentrations of cephradine in example 2;
FIG. 7 is a graph showing the change in chlorophyll content of Chlorella L166 under different concentrations of cephradine in example 2;
FIG. 8 is a graph showing the change in carotenoid content of Chlorella L166 under different concentrations of cephradine in example 2.
FIG. 9 is a graph showing the results of the degradation rates of comparative examples Chlorella L166(a) and Scenedesmus quadricaudatus (b) for Sulfadimethoxine (SDM) at different concentrations;
FIG. 10 is a graph of the biomass concentration of the comparative examples Chlorella L166(a) and Scenedesmus quadricauda (b) against different concentrations of Sulfadimethoxine (SDM);
FIG. 11 is a graph showing the change in chlorophyll content of comparative examples Chlorella L166(a) and Scenedesmus quadricaudatus (b) against Sulfadimethoxine (SDM) at different concentrations;
FIG. 12 is a graph showing the carotenoid content changes of comparative examples Chlorella L166(a) and Scenedesmus quadricaudatus (b) for different concentrations of Sulfadimethoxine (SDM).
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings and preferred embodiments.
Example 1: a method for strengthening microalgae purification of wastewater containing high-concentration antibiotics by utilizing ultraviolet strengthening comprises the following steps:
1) inoculating Scenedesmus quadratus FACHB-44 into 200mL of sterilized BG-11 culture medium, placing the culture medium into a biochemical incubator for pre-culture to enable Scenedesmus quadratus to reach a logarithmic growth phase, and then taking the Scenedesmus quadratus as a working algae solution;
the conditions of the pre-culture were set as: illumination all day long, illumination intensity 4800-2And N2Mixed gas of (2), CO2The occupied volume fraction is 5%.
2) And washing and centrifuging the algae liquid obtained in the step 1) by deionized water for 3 times, inoculating the algae liquid into artificial simulated high-concentration antibiotic wastewater with different concentrations, and culturing the wastewater on a microalgae culture rack. Ensuring that the absorbance of the initially inoculated microalgae is 0.1 at the wavelength of 680nm, illuminating all day by 4800-. The preparation method of the artificial simulated high-concentration antibiotic wastewater is that a certain amount of 4g/L cephradine mother liquor is added into 200mL BG-11 culture medium to obtain the artificial simulated high-concentration antibiotic wastewater containing 25, 50 and 100mg/L cephradine.
3) Periodically collecting the algae liquid cultured in the step 2), measuring the absorbance of the algae liquid at the wavelength of 680nm, and converting the algae liquid into the dry weight of cells according to a standard curve of the dry weight and the absorbance of the scenedesmus tetracaudatum, wherein the standard curve of the dry weight and the absorbance of the scenedesmus tetracaudatum is as follows: scenedesmus tetracaudatus dry cell weight (g/L) ═ 0.446 XOD680-0.006，R2＝0.999。
4) Periodically collecting the algae solution cultured in the step 2), centrifuging and removing the supernatant to obtain algae mud, re-suspending the harvested algae mud in methanol-water solution with the same volume, storing at 4 ℃ for 16-24h, centrifuging to determine the absorbance of the supernatant at multiple wavelengths, determining the concentration of intracellular photosynthetic pigment, and comparing the concentration with the cell dry weight to obtain the normalized intracellular photosynthetic pigment content.
FIG. 1 shows that after 8 days of culture, the microalgae have purification rates of 96.89 + -0.14%, 98.32 + -0.40% and 94.45 + -0.12% for cephradine in 25, 50 and 100mg/L artificial simulated wastewater.
FIG. 2 shows that after 8 days of culture, the biomass of microalgae purified from 25, 50 and 100mg/L artificial simulated wastewater containing cephradine is 122.32 + -0.32 mg/L, 117.86 + -0.32 mg/L and 111.847 + -1.26 mg/L, respectively.
After 8 days of culture, the chlorophyll contents of the microalgae in the processes of purifying cephradine from 25mg/L, 50 mg/L and 100mg/L artificial simulated wastewater are respectively 20.00 +/-1.58 mg/g, 21.83 +/-0.45 mg/g and 22.00 +/-0.78 mg/g, which are respectively increased by 47.09%, 60.54% and 61.78% compared with a control group.
FIG. 4 shows that after 8 days of culture, the carotenoids of microalgae in the process of purifying cephradine from artificial simulated wastewater of 25mg/L, 50 mg/L and 100mg/L are respectively 7.36 + -0.26 mg/g, 7.69 + -0.20 mg/g and 7.69 + -0.50 mg/g, which are respectively improved by 22.25%, 27.68% and 27.59% compared with the control group.
BG-11 is a control group, which differs from the examples in that the medium does not require the addition of antibiotics.
Example 2: embodiment 2 is different from embodiment 1 only in that chlorella L166 is used instead of scenedesmus tetracaudatus.
FIG. 5 shows that the purification rate of chlorella L166 to cefradine waste water of 25, 50 and 100mg/L is 96.95 + -1.19%, 95.74 + -0.52% and 86.32 + -0.04% after 8 days of culture.
FIG. 6 shows that the biomass of chlorella L166 in purified waste water of 25, 50 and 100mg/L cephradine after 8 days of culture is 134.32 + -2.55 mg/L, 138.19 + -0.37 mg/L and 127.23 + -0.91 mg/L respectively.
FIG. 7 shows that after 8 days of culture, the chlorophyll contents of Chlorella L166 in purified cefradine wastewater of 25mg/g, 50mg/g and 100mg/L are respectively 18.78 + -0.38 mg/g, 18.97 + -0.24 mg/g and 18.06 + -0.03 mg/g, which are respectively increased by 15.86%, 17.07% and 11.44% compared with the control group.
FIG. 8 shows that after 8 days of culture, the carotenoids of chlorella L166 in the process of purifying cefradine wastewater of 25mg/L, 50 mg/L and 100mg/L are respectively 6.68 + -0.01 mg/g, 6.80 + -0.03 mg/g and 6.52 + -0.14 mg/g, which are respectively improved by 14.39%, 16.50% and 11.60% compared with the control group.
Comparative example: the comparative example only differs from the examples in that Sulfadimethoxine (SDM) is used instead of cefradine.
Chlorella L166(a) and Scenedesmus quadricauda (b) showed removal of Sulfadimethoxine (SDM) at different concentrations as shown in FIG. 9. As can be seen from the figure, after 12 days of culture, the maximum removal rate of 25mg/L sulfadimethoxine by the chlorella L166 is 13.96%, and the maximum removal rate of 100mg/L sulfadimethoxine is 13.44%. At day 6 of culture, Scenedesmus quadricauda reached the maximum removal of SDM at low (25mg/L) and high (100 mg/L) concentrations, 11.43% and 4.01%, respectively.
The growth of chlorella cells exposed to different concentrations of SDM is shown in figure 10. On day 12, exposure to 25, 50, 100mg/L SDM reduced the dry weight of Chlorella L166 by 2.02%, 7.90% and 16.94% compared to the control. After 12 days, exposure to 25, 50, 100mg/L SDM reduced the dry weight of Scenedesmus quadricaudatus 17.28%, 40.03%, 53.42% compared to the control (136.35mg/L)
The photosynthetic pigment content of the two algae varied with SDM concentration is shown in fig. 11. On day 12, when exposed to SDM at 25, 50, 100mg/L, the total chlorophyll content of Chlorella L166 is 23.33 + -1.56 mg/g, 23.87 + -1.27 mg/g, 22.15 + -1.03 mg/g. SDM decreased only 4.80% at 100 mg/L. The chlorophyll content of Scenedesmus quadricaudatus on day 12, when exposed to SDM of 25, 50, 100mg/L, was 5.54 + -0.15 mg/g, 4.66 + -0.51 mg/g, 6.26 + -1.21 mg/g, which were respectively reduced by 49.01%, 57.11%, 42.42%
FIG. 12 shows the change in carotene content of algae exposed to different concentrations of SDM as the culture time was extended. Chlorella L166 carotenoid content was 6.49 + -0.25 mg/g, 6.58 + -0.15 mg/g, 6.23 + -0.32 mg/g, respectively, with a significant 4.80% decrease in SDM at 100mg/L only, when exposed to 25, 50, 100mg/L SDM, compared to the control group (6.53mg/g) on day 12. At day 12, scenedesmus tetracaudatus carotenoid content decreased by 19.88%, 25.22%, 38.22% respectively when exposed to 25, 50, 100mg/L SDM compared to the control group (6.883mg/g), 5.51 + -0.61 mg/g, 5.14 + -0.13 mg/g, 4.25 + -1.08 mg/g respectively.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A method for purifying cefradine wastewater based on microalgae culture is characterized by comprising the following steps: 1) pre-culturing functional microalgae to reach logarithmic growth phase;
2) and inoculating the functional microalgae in the logarithmic growth phase in the step 1) into the wastewater containing the cefradine antibiotic to purify the antibiotic wastewater.
2. The method for purifying cephradine wastewater based on microalgae cultivation as claimed in claim 1, wherein the functional microalgae is Scenedesmus quadricauda or Chlorella L166.
3. The method for purifying cephradine-containing wastewater based on microalgae cultivation according to claim 1, wherein the concentration of cephradine in step 2) is 25-100 mg/L.
4. The method for purifying cephradine wastewater based on microalgae cultivation as claimed in claim 1, wherein the pre-cultivation conditions are set as follows: illumination all day long, illumination intensity 4800-2And N2Mixed gas of (2), CO2The occupied volume fraction is 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111600388.5A CN114561293B (en) | 2021-12-24 | 2021-12-24 | Method for purifying cefradine wastewater based on microalgae culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111600388.5A CN114561293B (en) | 2021-12-24 | 2021-12-24 | Method for purifying cefradine wastewater based on microalgae culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114561293A true CN114561293A (en) | 2022-05-31 |
| CN114561293B CN114561293B (en) | 2023-11-28 |
Family
ID=81711951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111600388.5A Active CN114561293B (en) | 2021-12-24 | 2021-12-24 | Method for purifying cefradine wastewater based on microalgae culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114561293B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107629961A (en) * | 2017-10-16 | 2018-01-26 | 韶关学院 | A kind of method using breeding wastewater culture microalgae |
-
2021
- 2021-12-24 CN CN202111600388.5A patent/CN114561293B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107629961A (en) * | 2017-10-16 | 2018-01-26 | 韶关学院 | A kind of method using breeding wastewater culture microalgae |
Non-Patent Citations (2)
| Title |
|---|
| KE YANGA ET AL.: "An integrated view of the intimate coupling UV irradiation and algal treatment on antibiotic: Compatibility, efficiency and microbic impact assessment", JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, vol. 5, pages 4262 - 4268 * |
| 杜迎翔等, 环境科学与技术, vol. 38, no. 10 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114561293B (en) | 2023-11-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9416347B2 (en) | Method of treating bacterial contamination in a microalgae culture with pH shock | |
| CN113292164B (en) | Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio | |
| CN103937726A (en) | Alga-lysing pseudomonas aeruginosa and application thereof | |
| CN114561293B (en) | Method for purifying cefradine wastewater based on microalgae culture | |
| WO2020078433A1 (en) | Method for treatment and resource utilization of acidic organic wastewater | |
| CN1340462A (en) | Bacillus subtilis method for biologically repairing culture environment of aquatic products | |
| Ahmad et al. | Batch Phytoremediation of Aquaculture Wastewater of Silver Barramundi (Lates calcarifer) Utilizing Green Microalgae; Chlorella sp. | |
| CN101723481A (en) | Method for efficiently inactivating microcystis aeruginosa by irradiating electron beams | |
| Velichkova | Effect of different nitrogen sources on the growth of microalgae Chlorella vulgaris cultivation in aquaculture wastewater. | |
| CN1702045A (en) | Preparation of bacterium for cleaning pool bottom and method for rehabilitation of aquaculture environment | |
| WO2020083071A1 (en) | Microalgae purification culture medium and method for separation and purification of microalgae | |
| CN109133354B (en) | Algae-based ecological bacteriostasis method for wastewater treatment | |
| CN113214996A (en) | Scenedesmus as well as culture method and application thereof | |
| CN106967610B (en) | Culture method of spherical phaeocystis | |
| CN114249500A (en) | Method for purifying high-concentration antibiotic wastewater by re-culturing microalgae through ultraviolet pretreatment | |
| CN1340464A (en) | Bacillus rigens method for biologically repairing culture environment of aquatic products | |
| CN117125839B (en) | Method for removing microplastic in sewage by utilizing microalgae-fungus symbiont | |
| CN111847607B (en) | Method for removing floating plants and phytoplankton | |
| CN107469269B (en) | Method for synergistically degrading polyethylene plastic by utilizing two bacteria | |
| RU2676153C2 (en) | Method of biodegradation of malachite green (options) | |
| CN117776411A (en) | Application of MR-1 Kaschin-Schwann EPS in degradation of plasticizer | |
| Zhua et al. | Nutritional pattern-driven algal antibiotic treatment: a period-specific and carbon-dependent removal process | |
| Wang et al. | Sulfur balance in the treatment of sulfur-containing wastewater in a biotrickling reactor | |
| CN115155587A (en) | Iron-aluminum mud-based magnetic catalyst, preparation method and algae removal method | |
| CN117143737A (en) | Method for culturing double-eyebrow alga by using hot pickled mustard tuber wastewater, double-eyebrow alga and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |