CN101723481A - Method for efficiently inactivating microcystis aeruginosa by irradiating electron beams - Google Patents
Method for efficiently inactivating microcystis aeruginosa by irradiating electron beams Download PDFInfo
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- CN101723481A CN101723481A CN200910199554A CN200910199554A CN101723481A CN 101723481 A CN101723481 A CN 101723481A CN 200910199554 A CN200910199554 A CN 200910199554A CN 200910199554 A CN200910199554 A CN 200910199554A CN 101723481 A CN101723481 A CN 101723481A
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Abstract
The invention relates to a method for efficiently controlling, eliminating and inactivating blue algae, particularly microcystis aeruginosa in the source water, the water in the water plant, the scenic lake water and the river water by irradiating electron beams, belonging to the field of the water pollution control, the water treatment technology and the environmental protection. The test proves that the electron beams are irradiated by an electronic irradiating device which has the electron beam irradiating energy of 1.8 MeV and the electron beam current of 1.0 mA at the room temperature of 25 DEG C and the atmospheric pressure of 1013.2 hpa, and the optimum dosage of the electron beams irradiated for eliminating or inactivating the microcystis aeruginosa is 4-5 kGy.
Description
Technical field:
The present invention relates to a kind of microcystic aeruginosa in the water body be controlled electron beam irradiation treatment process with deactivation, belong to water pollution control, water technology and field of environment protection.
Background technology
Blue-green alga bloom and toxin thereof are as the emphasis of body eutrophication pollution hazard, and in individual amount and the secretion of algae toxin, microcystic aeruginosa (Microcystis aerugiosa) occupies main ratio.Conventional water treatment processes such as the coagulation of tap water treatment process, precipitation, filtration, chlorination still can't effectively be removed the algae toxin in the water, physical method such as air supporting, membrane filtration and charcoal absorption etc. can only be transferred to another kind of medium to MCs from a kind of medium, and general chemical oxidation method such as chlorination, potassium permanganate oxidation exist formation three to cause the risk of disinfection byproduct (DBP).The plant allelopathy has better effects to control water body microcystic aeruginosa, but MC concentration is higher in water body, can reduce the photosynthetic oxygen evolution of high waterplant, and changes its photosynthetic pigments formation and enzymic activity.Seek a kind of effective method, can control and deactivation microcystic aeruginosa, the toxin that suppresses in the frustule produces and release, remedies the limitation that conventional water treatment process is removed the algae toxin.
Because pyrrole ring and phenyl ring in the algae chlorophyll are very similar, electron beam can make phenyl ring change and odorless, tasteless, be applied to pyrrole ring, can oxidation chlorophyll, and expectation is by stopping metabolism, interrupting synthetic protein and make microcystic aeruginosa death.
Summary of the invention
The objective of the invention is in order to solve traditional water treatment technology and,, propose a kind ofly to control electron beam irradiation treatment process with deactivation at microcystic aeruginosa for purifying and improving water quality removing defective and the deficiency that exists aspect harmful blue-green algae.
The present invention a kind ofly controls electron beam irradiation treatment process with deactivation to microcystic aeruginosa, it is characterized in that having following process and step:
The enlarged culturing of a, algae, the microcystic aeruginosa with obtaining in the microcystic aeruginosa pure strain buied or the water body is inoculated in " BG-11 substratum ", and inoculum size is 10%, and culture temperature is that 28 ℃, periodicity of illumination are 12:12 (h).Treat incubation growth to logarithmic phase, when range estimation nutrient solution color obviously turns green, can do laboratory sample, stand-by;
B, pour the above-mentioned algae liquid that is cultured to logarithmic phase into sterilized culture dish, under the environment that opens wide, be positioned on the electron beam irradiation device, carry out electron beam irradiation and handle.The electron beam irradiation energy is 1.8MeV, and line is 1.0mA, and a radiation dose is 1kGy.The radiation dose that adopts in the experiment is respectively 1kGy, 2kGy, 3kGy, 4kGy, 5kGy;
C, draw the growth curve behind the irradiation: take out the algae liquid of cultivating with the sterilization transfer pipet every day at one time, measures its optical density(OD) under the specific absorption wavelength and different irradiation doses and time relation curve, and the dosage range of definite effective irradiation therefrom;
D, simultaneously, finish in back 2 hours in radiation, will be fixing at one time through the frustule of radiotreatment and the normal frustule of radiating, adopt transmission microscopy to observe the variation of frustule, according to the useful range of the further clear and definite irradiation dose of destructiveness of frustule.
E, process by experiment, drawing microcystic aeruginosa is controlled the optimal dose of handling with the electron beam irradiation of deactivation is 4 ~ 5kGy.
Method of the present invention is its result show, adopts electron beam irradiation deactivation microcystic aeruginosa effectively.Utilize " BG-11 substratum " enlarged culturing microcystic aeruginosa, with 28 ℃, periodicity of illumination is that the constant temperature culture of 12:12 (h) can make the living environment of algae more approach the physical environment of blue-green alga bloom outburst in incubator.Studies have shown that electron beam irradiation is very effective to the degradation capability of other organic pollutants, adopting said method can be explored the removal ability of electron beam irradiation aspect biological pollutant.Present method proves that electron beam irradiation not only can be controlled and deactivation microcystic aeruginosa, and the toxin that more can suppress in the frustule produces and release, remedies the limitation of conventional water treatment process to algae and the removal of algae toxin.Method of the present invention utilizes electron beam irradiation to remove microcystic aeruginosa, and its preparation cost is cheap, and treatment effect is good, in the engineering that adopts electron beam irradiation technical finesse source water and tap water bigger application potential is arranged.
Description of drawings
The graph of relation of Fig. 1 for about different radiation dose the microcystic aeruginosa optical density(OD) being influenced among the present invention.(also being the growth rhythm graphic representation of microcystic aeruginosa)
Fig. 2 is about the graph of relation of different radiation dose to microcystic aeruginosa chlorophyll a content influence among the present invention.
Embodiment
Now with specific embodiments of the invention narrations (is example with the microcystic aeruginosa):
(1), the cultivation of microcystic aeruginosa, the microcystic aeruginosa pure strain with buying is inoculated in " BG-11 substratum ", inoculum size is 10%, cultivates in 28 ℃, periodicity of illumination are the constant incubator of 12:12 (h).Treat incubation growth to logarithmic phase, when range estimation nutrient solution color obviously turns green, can do laboratory sample, stand-by;
(2), pour the above-mentioned algae liquid that is cultured to logarithmic phase into sterilized culture dish, under the environment that opens wide, be positioned on the electron beam irradiation device, carry out electron beam irradiation and handle.The electron beam irradiation energy is 1.8MeV, and line is 1.0mA, and a radiation dose is 1kGy.The radiation dose that adopts in the experiment is respectively 1kGy, 2kGy, 3kGy, 4kGy, 5kGy;
(3), draw the growth curve behind the irradiation: take out the algae liquid of cultivating with the sterilization transfer pipet every day at one time, measures its optical density(OD) under the specific absorption wavelength and different irradiation doses and time relation curve, and the dosage range of definite effective irradiation therefrom;
(4), simultaneously, finish in back 2 hours in radiation, will be fixing at one time through the frustule of radiotreatment and the normal frustule of radiating, adopt transmission microscopy to observe the variation of frustule, according to the useful range of the further clear and definite irradiation dose of destructiveness of frustule.
(5), process by experiment, drawing microcystic aeruginosa is controlled the optimal dose of handling with the electron beam irradiation of deactivation is 4 ~ 5kGy.
Analysis and evaluation to process of the test among the embodiment or treating processes: Figure 1 shows that the optical density curve of microcystic aeruginosa under the different radiation dose, also is growth rhythm.Among the figure, the optical density(OD) of control group (CK) increases with usual dispatch.The growth level of 1kGy radiation group is lower than control group, and after radiation the 3rd day, increasing degree increased to some extent, plays the original level of basic recovery on the 6th day.Low dose radiation stimulates and impels the increase of intracellular reactive oxygen, removes the antioxidant system of active oxygen and is induced enhancing, and the growth of cell is accelerated to some extent; The radiation dose of 2-5kGy makes the decline immediately in the 1st day after radiation of its algae liquid optical density(OD), and dosage is big more, and the decline degree is also big more; The rising of the 2nd day optical density(OD) can be understood as resistivity and the adaptability of Microcystis aeruginosa self to adverse circumstance after the radiation.After of short duration rise, the optical density(OD) of 2-5kGy radiation group remains decline.Test latter stage, control group and 1kGy radiation component have not increased by 1.082 and 0.407; The optical density(OD) of 2-5kGy radiation group descends and is respectively 0.533,0.731,0.889 and 0.946, and it is about 50% also to be that the 2-3kGy group descends, and the 4-5kGy group descends about 70%; This shows, the radiation dose energy deactivation microcystic aeruginosa of 2-5kGy, wherein the 4-5kGy effect is better.
Chlorophyll a is that algae carries out photosynthetic basic substance, and the height of its content has reflected the photosynthesis level of algae.As Fig. 2, the chlorophyll-a concentration of control group (CK) has increased 6.173ug/L; The chlorophyll a content of 1kGy radiation group is at experiment kept stable in early stage, and the later stage increases to some extent, and when experiment finished, concentration had increased 1.645ug/L, and amplitude is much smaller than control group; The radiostimulation of 2-3kGy radiation group, making in its chlorophyll a content 2 days after radiation has a small amount of increase, keeps afterwards descending; The radiation of 4-5kGy radiation group is comparatively abominable for microcystic aeruginosa, chlorophyll a content is decline respectively in the 1st day after radiation, reduce 3.488ug/L and 3.571ug/L respectively to latter stage, weakened the photosynthesis of algae largely, reached the purpose of control algal grown.
Claims (1)
1. one kind microcystic aeruginosa controlled electron beam irradiation treatment process with deactivation, it is characterized in that having process and step:
The enlarged culturing of a, algae, the microcystic aeruginosa with obtaining in the microcystic aeruginosa pure strain buied or the water body is inoculated in " BG-11 substratum ", and inoculum size is 10%, and culture temperature is that 28 ℃, periodicity of illumination are 12:12 (h).Treat incubation growth to logarithmic phase, when range estimation nutrient solution color obviously turns green, can do laboratory sample, stand-by;
B, pour the above-mentioned algae liquid that is cultured to logarithmic phase into sterilized culture dish, under the environment that opens wide, be positioned on the electron beam irradiation device, carry out electron beam irradiation and handle.The electron beam irradiation energy is 1.8MeV, and line is 1.0mA, and a radiation dose is 1kGy.The radiation dose that adopts in the experiment is respectively 1kGy, 2kGy, 3kGy, 4kGy, 5kGy;
C, draw the growth curve behind the irradiation: take out the algae liquid of cultivating with the sterilization transfer pipet every day at one time, measures its optical density(OD) under the specific absorption wavelength and different irradiation doses and time relation curve, and the dosage range of definite effective irradiation therefrom;
D, simultaneously, finish in back 2 hours in radiation, will be fixing at one time through the frustule of radiotreatment and the normal frustule of radiating, adopt transmission microscopy to observe the variation of frustule, according to the useful range of the further clear and definite irradiation dose of destructiveness of frustule.
E, process by experiment, drawing microcystic aeruginosa is controlled the optimal dose of handling with the electron beam irradiation of deactivation is 4 ~ 5kGy.
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Cited By (4)
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| CN111718045A (en) * | 2020-07-07 | 2020-09-29 | 清华大学 | Water body algae inhibiting method |
| CN111874991A (en) * | 2020-06-20 | 2020-11-03 | 重庆大学 | Method for inactivating microcystis aeruginosa cells by ultrasonic radiation |
| CN113142279A (en) * | 2021-04-12 | 2021-07-23 | 宁波大学 | Protein liquid suitable for seafood-flavored baked food and preparation method thereof |
| WO2023142201A1 (en) * | 2022-01-29 | 2023-08-03 | 成都威斯克生物医药有限公司 | Method for industrial production of vaccine against pseudomonas aeruginosa |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101139140A (en) * | 2007-08-23 | 2008-03-12 | 复旦大学 | Aerugo microcapsule algae degradation method using animalcule |
| CN101230322A (en) * | 2007-09-11 | 2008-07-30 | 南开大学 | Novel medical stone micro-ecological repairing additive and preparation method thereof |
| CN101390526A (en) * | 2008-11-06 | 2009-03-25 | 天津农学院 | Microcystic aeruginosa Chinese herbal medicine algae-inhibition agent and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111874991A (en) * | 2020-06-20 | 2020-11-03 | 重庆大学 | Method for inactivating microcystis aeruginosa cells by ultrasonic radiation |
| CN111718045A (en) * | 2020-07-07 | 2020-09-29 | 清华大学 | Water body algae inhibiting method |
| CN111718045B (en) * | 2020-07-07 | 2021-06-15 | 清华大学 | Water body algae inhibiting method |
| CN113142279A (en) * | 2021-04-12 | 2021-07-23 | 宁波大学 | Protein liquid suitable for seafood-flavored baked food and preparation method thereof |
| CN113142279B (en) * | 2021-04-12 | 2023-08-18 | 宁波大学 | Protein liquid suitable for seafood-flavored baked food and preparation method thereof |
| WO2023142201A1 (en) * | 2022-01-29 | 2023-08-03 | 成都威斯克生物医药有限公司 | Method for industrial production of vaccine against pseudomonas aeruginosa |
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