CN110182960A - A method of black and odorous water is handled using microalgae - Google Patents
A method of black and odorous water is handled using microalgae Download PDFInfo
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- CN110182960A CN110182960A CN201910533377.6A CN201910533377A CN110182960A CN 110182960 A CN110182960 A CN 110182960A CN 201910533377 A CN201910533377 A CN 201910533377A CN 110182960 A CN110182960 A CN 110182960A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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Abstract
The present invention provides a kind of methods using microalgae processing black and odorous water, belong to ecological environmental protection technical field.Microalgae is inoculated in black and odorous water by the present invention, 20~30 DEG C of 10~20d of culture.Experiment shows that microalgae (especially boat-shaped algae) can be grown using the pollutant component in black and odorous water water sample, adds nutritive salt without external；Microalgae is applied to the processing of black and odorous water, the treatment cost of black and odorous water can be reduced；Microalgae energy deep purifying water quality during absorbing the polluter in the black and odorous water of city, water conservation are not only environmentally friendly but also inexpensive.
Description
Technical field
The invention belongs to ecological environmental protection technical fields, and in particular to a kind of side using microalgae processing black and odorous water
Method.
Background technique
Water quality ecological restorative procedure is generally divided into physical method, chemical method and biological-ecological method, controls in polluted-water
Oneself obtains certain achievement to reason aspect.Physical method includes that dirt, Sediment Dredging, river oxygenation, running water circulation and clear water are cut in control source
The methods of supply and bypass processing.These methods are only used for the emergency processing of water body and the pre-treatment of Ecological Disposal, it is difficult to
The stabilization of water quality after holding processing.Chemical method mainly includes depollution of being made a return journey using chemical floc and chemical algae removing agent
Object, control algal grown, achieve the effect that purify water.But in view of chemicals environment side effect and use cost with
Continue timeliness, chemical method can only be more common in Emergency use as a kind of measure of auxiliary.
It is biological prosthetic to be used as a kind of Low investment, height with the raising of environmental requirement and the continuous development of Environmental Biotechnology
Benefit, operation water pollution Treatment process flexible to operation gradually develop and are applied in production.Wherein bio-ecological process
It mainly include water plant recovery technique and adding bacteria agent etc..Biological-ecological technology more Environmental Safety, but water plant
It will cause more after rotting after a large amount of water plant is dead in restorative procedure as cleaned up easily blocking river, plant residue not in time
Big river pollution；It is repaired by adding external strain, may there is existence between the indigenous microorganism in water body
Competition will lead to the activity and the reduction of biomass of indigenous microorganism degradation of contaminant, to influence water remediation effect.
Summary of the invention
The problem of in view of background technique, handles black smelly water using microalgae the purpose of the present invention is to provide a kind of
The method of body.
The present invention provides a kind of methods using microalgae processing black and odorous water, microalgae are inoculated in black and odorous water, 20
~30 DEG C of 10~20d of culture.
Preferably, the microalgae includes one of scenedesmus, boat-shaped algae and small ring algae or a variety of.
Preferably, the density of the microalgae inoculation is 1~3 × 105A/mL.
Preferably, the Light To Dark Ratio of the culture is 11~13:11~13.
Preferably, intensity of illumination when optical culture is 2500~3500Lux.
Preferably, the microalgae is boat-shaped algae；The density of the microalgae inoculation is 1.5~2.5 × 105A/ml；The training
Feeding Light To Dark Ratio is 11.5~12.5:11.5~12.5；Intensity of illumination when optical culture is 2800~3200Lux；Cultivation temperature
It is 23~27 DEG C, incubation time is 13~17d.
The utility model has the advantages that microalgae is inoculated in black smelly the present invention provides a kind of method using microalgae processing black and odorous water
In water body, 20~30 DEG C of 10~20d of culture.Experiment shows that microalgae (especially boat-shaped algae) can be using in black and odorous water water sample
Pollutant component is grown, and adds nutritive salt without external；Microalgae is applied to the processing of black and odorous water, black smelly water can be reduced
The treatment cost of body；Microalgae energy deep purifying water quality during absorbing the polluter in the black and odorous water of city, protection water money
Source, it is not only environmentally friendly but also inexpensive.
Detailed description of the invention
Fig. 1 is the algae density of three kinds of microalgaes 0d, 7d, 15d in black smelly water sample；
Fig. 2 is contaminants removal rate of three kinds of microalgaes in black smelly water sample；
Fig. 3 is pH, dissolved oxygen, ORP variation of three kinds of microalgaes in black smelly water sample；
Specific embodiment
The present invention provides a kind of methods using microalgae processing black and odorous water, microalgae are inoculated in black and odorous water, 20
~30 DEG C of 10~20d of culture.
Microalgae is inoculated in black and odorous water and cultivates by the present invention.
In the present invention, the microalgae preferably includes one of scenedesmus, boat-shaped algae and small ring algae or a variety of, more preferably
Boat-shaped algae.The present invention is not particularly limited the specific source of microalgae, and this field conventional acquisition methods are such as bought or acquired
It can.In an embodiment of the present invention, the scenedesmus is scenedesmus LX1 (Scenedesmus sp.LX1), by Tsing-Hua University's environmental
Laboratory separates acquisition in sub-nutrition environment；The small ring algae (Cyclotella sp.) and boat-shaped algae (Navicula sp.)
It is purchased from Chinese Academy of Sciences's fresh water algae library, is stored in CSI culture medium in laboratory.
In the present invention, the black and odorous water is the black and odorous water in practical river, and water quality indicator is bad V class water, gently
It is black smelly to spend black smelly or severe.
In the present invention, the density of the microalgae inoculation is preferably 1~3 × 105A/mL, more preferably 1.5~2.5 ×
105A/ml, more preferably 2 × 105A/mL.The temperature of the culture be 20~30 DEG C, preferably 23~27 DEG C, more preferably
25℃.The Light To Dark Ratio of the culture is preferably 11~13:11~13, is more orderly 11.5~12.5:11.5~12.5, more preferably
For 12:12.Intensity of illumination when optical culture is preferably 2500~3500Lux, more preferably 2800~3200Lux, more preferably
3000Lux.The time of the culture is 10~20d, more preferably 13~17d, more preferably 15d.
The present invention handles black and odorous water using microalgae, and microalgae can be black smelly by converting using black and odorous water as culture substrate
Polluter in water body is proliferated, while deep purifying water quality, water conservation.In addition, microalgae nothing in breeding
External nutritive salt need to be added, its treatment cost can be reduced.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
(1) it fetches water from Shanghai Songjiang, obtains tetra- water samples of A, B, C and D, specific water quality is shown in Table 1.
1 water sample source of table and condition of water quality
In table 1,1 and 2 represent different rivers, and there are two different sampled points respectively in every river.Four water samples are
Bad V class water, wherein two water samples of 1# are all slight black smelly, and two water samples of 2# are that severe is black smelly.
(2) scenedesmus, small ring algae and boat-shaped algae are respectively inoculated in from tetra- black and odorous water water samples of A, B, C and D respectively
Test bottle in, control algae inoculum density be 2 × 105A/mL is 12:12, intensity of illumination 3000Lux, temperature in Light To Dark Ratio
15d is cultivated under conditions of being 25 DEG C, the water after collection and purification.
(3) growth curve of microalgae in each experimental group, ammonia nitrogen, total nitrogen, total phosphorus, COD, PH, oxygen in purified water are measured
Change reduction potential and dissolved oxygen concentration.Wherein, algae density measurement method uses cell counting, that is, takes a certain amount of algae solution in whirlpool
It is mixed on oscillator, uses ascites method afterwards, counted under the microscope with blood counting chamber within every two days, measured
Algae density simultaneously draws algae growth curve.
As a result as shown in Figures 1 to 3, in which:
Fig. 1 is the algae variable density situation that three kinds of microalgaes are cultivated wherein the 0th day, the 7th day and the 15th day 15 days in water sample；
As shown in Figure 1, after three kinds of microalgaes cultivate 15 days in water sample, algae density has significant growth.Wherein, the algae density highest of scenedesmus
Up to 13.3 × 105A/mL, boat-shaped algae reach as high as 8.3 × 105A/mL, small ring algae reach as high as 7.1 × 105A/mL.Grid
The algae density of algae is highest in three kinds of microalgaes, and growing way is best, and boat-shaped algae and small ring algae are slightly lower.
Fig. 2 is that three kinds of microalgaes cultivate the pollutants removal rate after 15 days in water sample；As shown in Figure 2, three kinds of microalgaes are black
After cultivating 15 days in smelly water body, the ammonia nitrogen in water sample can be effectively removed.Wherein, the ammonia nitrogen removal frank of scenedesmus reaches as high as
79.56%, boat-shaped algae reaches as high as 100%, and small ring algae reaches as high as 100%.The ammonia nitrogen removal frank of boat-shaped algae and small ring algae is equal
It can reach 100%, but the ammonia nitrogen removal frank highest that boat-shaped algae is overall in four water samples, removal effect are best.Three kinds of microalgaes exist
After cultivating 15 days in black and odorous water, the total nitrogen in water sample can be effectively removed.Wherein, the nitrogen removal rate of scenedesmus reaches as high as
71.79%, boat-shaped algae reaches as high as 66.67%, and small ring algae reaches as high as 51.28%.The nitrogen removal rate highest of scenedesmus, boat-shaped
Algae is slightly below scenedesmus, and small ring algae is minimum.After three kinds of microalgaes cultivate 15 days in black and odorous water, can it effectively remove total in water sample
Phosphorus.Wherein, total tp removal rate of scenedesmus reaches as high as 67.11%, and boat-shaped algae reaches as high as 100%, and small ring algae reaches as high as
100%.Total tp removal rate of boat-shaped algae and small ring algae can reach 100%, but the total phosphorus that boat-shaped algae is overall in four water samples
Removal rate highest, removal effect are best.After three kinds of microalgaes cultivate 15 days in black and odorous water, can it effectively remove in water sample
COD.Wherein, the nitrogen removal rate of scenedesmus reaches as high as 75%, and boat-shaped algae reaches as high as 81.82%, and small ring algae reaches as high as
79.55%.The COD average removal rate and highest removal rate of boat-shaped algae are highest in three kinds of microalgaes, and removal effect is best.
Fig. 3 is pH, the dissolved oxygen concentration, ORP variation after three kinds of microalgaes are cultivated 15 days in water sample；From the figure 3, it may be seen that three kinds
After microalgae cultivates 15 days in black and odorous water, water sample pH has different degrees of growth.Wherein, raw water sample pH average value is 7.10,
PH average value is 9.58 after scenedesmus processing, and pH average value is 8.06 after the processing of boat-shaped algae, and pH average value is after small ring algae processing
8.25.Water sample pH is closest neutral after the processing of boat-shaped algae.After three kinds of microalgaes cultivate 15 days in black and odorous water, water sample redox
Current potential has significant growth.Wherein, raw water sample ORP average value is -81.75, and ORP average value is 158 after scenedesmus processing, boat-shaped algae
ORP average value is 190.5 after processing, and ORP average value is 148.75 after the processing of small ring algae, and the ORP of water sample is most after the processing of boat-shaped algae
Height can reach 200.After three kinds of microalgaes cultivate 15 days in black and odorous water, water sample dissolved oxygen has significant growth.Wherein,
Raw water sample dissolved oxygen average value is 1.44mg/L, and dissolved oxygen average value is 3.46mg/L after scenedesmus processing, molten after the processing of boat-shaped algae
Solution oxygen average value is 4.76mg/L, and dissolved oxygen average value is 4.51mg/L after small ring algae processing.Water sample is molten after the processing of boat-shaped algae
Oxygen highest is solved, oxygenation effect is best.
From Fig. 1~3: the maximum algae density that boat-shaped algae grows in black and odorous water can reach 8.3 × 105A/mL, ammonia
Nitrogen removal efficiency is up to 100%, and for nitrogen removal rate up to 66.67%, total tp removal rate is reachable up to 100%, COD removal rate
81.82%, pH value 8.06, dissolved oxygen concentration is up to 200mg/L.Boat-shaped algae handles the effect of black and odorous water in three kinds of microalgaes
It is best.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of method using microalgae processing black and odorous water, which is characterized in that microalgae is inoculated in black and odorous water, 20~30
DEG C culture 10~20d.
2. the method according to claim 1, wherein the microalgae includes in scenedesmus, boat-shaped algae and small ring algae
It is one or more.
3. the method according to claim 1, wherein the density of microalgae inoculation is 1~3 × 105A/mL.
4. the method according to claim 1, wherein the Light To Dark Ratio of the culture is 11~13:11~13.
5. according to the method described in claim 4, it is characterized in that, intensity of illumination when optical culture is 2500~3500Lux.
6. method described in any one according to claim 1~5, which is characterized in that the microalgae is boat-shaped algae；The microalgae
The density of inoculation is 1.5~2.5 × 105A/ml；The Light To Dark Ratio of the culture is 11.5~12.5:11.5~12.5；Optical culture
When intensity of illumination be 2800~3200Lux；Cultivation temperature is 23~27 DEG C, and incubation time is 13~17d.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110723871A (en) * | 2019-10-29 | 2020-01-24 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
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| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
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| CN108467149A (en) * | 2018-02-06 | 2018-08-31 | 安徽科耀环保科技有限公司 | A kind of method of black and odorous water processing |
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| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
| CN104513799A (en) * | 2013-09-29 | 2015-04-15 | 重庆工商大学 | New idea of utilizing sewage to culture oil-rich algae and also purify water |
| CN105645595A (en) * | 2016-01-13 | 2016-06-08 | 新奥科技发展有限公司 | Treatment method of black foul water body |
| CN107827239A (en) * | 2017-04-21 | 2018-03-23 | 湖南大学 | It is a kind of to utilize dilute floating pulse duckweed and the in-situ remediation method of chlorella ellipsoidea Combined Treatment black and odorous water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110723871A (en) * | 2019-10-29 | 2020-01-24 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
| CN110723871B (en) * | 2019-10-29 | 2022-04-22 | 山东理工大学 | Method for treating black and odorous water by combining indigenous microbial film and microalgae |
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