CN104604951A - Method for inhibiting growth of microcystis by using falling litchi leaves - Google Patents
Method for inhibiting growth of microcystis by using falling litchi leaves Download PDFInfo
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- CN104604951A CN104604951A CN201510017911.XA CN201510017911A CN104604951A CN 104604951 A CN104604951 A CN 104604951A CN 201510017911 A CN201510017911 A CN 201510017911A CN 104604951 A CN104604951 A CN 104604951A
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Abstract
The invention discloses a method for inhibiting growth of microcystis by using falling litchi leaves. The method comprises the following steps: A, drying and rubbing washed falling litchi leaves, and soaking by using distilled water to prepare a water extract of the falling litchi leaves for standby use; B, inoculating the microcystis in a logarithmic phase into a BG-11 culture medium to prepare a microcystis culture solution; C, adding the water extract of the falling litchi leaves into the prepared microcystis culture solution, putting the liquid into an illumination incubator, and carrying out culturing in a light and dark alternate way so as to inhibit the growth of the microcystis through the water extract of the falling litchi leaves. The method is simple in operation and low in treatment cost; according to the method, wastes can be reused, control over excessive propagation of typical algae in a freshwater system is realized, and the safety of the environment of the freshwater system is ensured; the method has a broad application prospect for controlling microcystis bloom.
Description
Technical field
The present invention relates to algae control technology field, especially a kind of method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water.
Background technology
The entering of a large amount of industrial wastewater and sanitary sewage in recent years, accelerate the eutrophication process of lake and reservoir, " wawter bloom " frequently occurs, area spreads year by year, duration extension.What large-scale breakout of cyanobacteria blooms reduced water resource utilizes usefulness, as caused, water quality is corrupt, Algae toxins affect " three cause " material that the health of the mankind, some algae and metabolite thereof produce in sterilization, algae filling waterworks by food chain filter, some frustules destruction flocculation process etc.Therefore, effectively control the algae in eutrophication water, prevent the study hotspot becoming current environmental area and the forward position of wawter bloom.
20 century 70s, allelopathy is that a Plants (comprising microorganism) is by being harmful to or useful effect to Environment release chemical substance to other individual generations.Research shows, most of allelochemical not only biodegradable, and shorter than the agricultural chemicals half life period of synthesis, and more traditional pollution of herbicide is less.Allelopathic Effect in Plants is considered to a kind of novel biological algal control technology, has the features such as efficient, economic, ecological risk is little, enjoys domestic and international concern in recent years.Allelopathy removes the recent development that algae is biological algae removal technology, and plant is allelochemical by discharging chemical substance in environment, and it is the metabolite of plant, easily degrades in water body, limited to water pollution; And plant origin is extensive, can obtain different allelochemical from different plant; Allelochemical is microgram/L or following except the effective dose of algae, has high efficiency; Allelochemical is generally algal control type, non-ly kills algae type reagent, and do not destroy frustule, cellular content does not enter water body, and sludge yield is few, is beneficial to subsequent treatment.
At present, the research utilizing water plants to control wawter bloom mainly concentrates on submerged plant, emergent aquactic plant and floating plant, and makes some progress.But, relatively less to the research of terrestrial plant allelopathic control algae at present, there is not the concrete research effectively suppressing Microcystis aeruginosa for magaphanerophytes.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of the weak point of existing algae control technology, the object of the present invention is to provide and a kind ofly utilize lichee to fall leaves to suppress the method for Growth of Microcystis in Water, be intended to solve in existing algae-removing technology and there is high cost, secondary pollution, selectivity and validity difference and the problem that can cause ecological disruption.
In order to achieve the above object, this invention takes following technical scheme:
Utilize lichee to fall leaves and suppress a method for Growth of Microcystis in Water, wherein, comprise the following steps:
A, the lichee fallen leaves after cleaning are carried out drying, rub broken process, and soak with distilled water, prepare lichee fallen leaves water-leach liquor stand-by;
B, the Microcystis aeruginosa being in exponential phase is inoculated in BG-11 medium, preparation Microcystis aeruginosa culture fluid;
C, in above-mentioned prepared Microcystis aeruginosa culture fluid, add lichee fallen leaves water-leach liquor, and be placed in illumination box and cultivate with illumination and the dark mode replaced, suppressed the growth of Microcystis aeruginosa by lichee fallen leaves water-leach liquor.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step, the mass concentration of described lichee fallen leaves water-leach liquor is 1.0-3.0 gL
-1.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in stepb, described BG-11 medium is formulated by following component: NaNO
31.5g, K
2hPO
40.04g, MgSO
47H
2o 0.075g, CaCl
27H
2o 0.036g, Na
2cO
30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution 1mL, and be settled to 1000 mL with distilled water.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, described trace element solution is formulated by following component: H
3bO
40.286g, MnCl
24H
2o 0.181g, ZnSO
40.0222g, Na
2moO
40.039g, CuSO
45H
2o 0.0079g, Co (NO
3)
26H
2o 0.00494g, and be settled to 100 mL with distilled water.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in stepb, in described Microcystis aeruginosa culture fluid, the initial density of Microcystis aeruginosa is 1.0 × 10
5-1.3 × 10
6cellmL
-1.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step C, the Light To Dark Ratio in described illumination box is 12h:12h.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step C, the cultivation temperature in described illumination box is 25-28 DEG C.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step C, the intensity of illumination in described lighting box is 30 μm of ol m
-2s
-1.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step C, the incubation time in described illumination box is 14-16d.
The described method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, wherein, in step, the concrete preparation process of described lichee fallen leaves water-leach liquor is:
S1, collect lichee fallen leaves and rinse well with water, being positioned in 40 DEG C of baking ovens to dry after 4 ~ 5 d and taking out, be cut into segment and rub that to be broken into broken fallen leaves sheet stand-by;
S2, being soaked in distilled water by rubbing broken broken blade, being positioned in climatic cabinate with after sealed membrane sealing, and placing 4 ~ 5 d under dark surrounds, through miillpore filter filtration under diminished pressure, obtain lichee fallen leaves water-leach liquor.
Beneficial effect: the present invention passes through with lichee fallen leaves as raw material, prepare lichee fallen leaves water-leach liquor, and water-leach liquor of being fallen leaves by lichee is rendered in pending Microcystis aeruginosa medium, to suppress Growth of Microcystis in Water, described utilize lichee to fall leaves to suppress the method selectivity of Growth of Microcystis in Water and validity strong, and it can not cause secondary pollution.
Accompanying drawing explanation
Fig. 1 is the method flow diagram utilizing lichee fallen leaves to suppress Growth of Microcystis in Water of the present invention.
Embodiment
The invention provides a kind of method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, for making object of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
As shown in Figure 1, the invention provides a kind of method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water, it specifically comprises the following steps:
S100, the lichee fallen leaves after cleaning are carried out drying, rub broken process, and soak with distilled water, prepare lichee fallen leaves water-leach liquor stand-by;
S200, the Microcystis aeruginosa being in exponential phase is inoculated in BG-11 medium, preparation Microcystis aeruginosa culture fluid;
S300, in above-mentioned prepared Microcystis aeruginosa culture fluid, add lichee fallen leaves water-leach liquor, and be placed in illumination box and cultivate with illumination and the dark mode replaced, suppressed the growth of Microcystis aeruginosa by lichee fallen leaves water-leach liquor.
Lichee is southern china a kind of aiphyllium plant distributed more widely, have cultivated area wide, cultivate the features such as extensive, the life-span is long, the fallen leaves of lichee described in the present invention can select the fallen leaves of litchi, and it has that material source is wide, quantity is large, Financial cost is low and has the advantages such as twice laid concurrently.Adopt lichee fallen leaves water-leach liquor effectively can suppress the growth of Microcystis aeruginosa, only do not need the lichee fallen leaves water-leach liquor dropping into very low concentrations can suppress the growth of Microcystis aeruginosa, and without the need to dropping into other chemical reagent, thus the sludge quantity caused after avoiding other chemical reagent of interpolation increases and treating capacity increases, set up the water ecology adjustment and control system based on lichee fallen leaves water-leach liquor algal control mechanism, there is potential practical value.
In addition, Microcystis aeruginosa described in the present invention is the microcystic aeruginosa being in logarithmic phase, its color is bottle green, and there is the fast and feature that vitality is strong of fast growth, breeding, select the microcystic aeruginosa of logarithmic phase as experimental subjects, the suppression situation of lichee fallen leaves to Growth of Microcystis in Water can be obtained accurately and rapidly.Preferably, described Microcystis aeruginosa can also select the microcystic aeruginosa being in different growth periods, so that the growth inhibition situation of complete observation lichee fallen leaves to Microcystis aeruginosa.
By the method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water of the present invention, water-leach liquor of being fallen leaves by lichee is rendered in the BG-11 medium containing Microcystis aeruginosa, containing allelochemical in lichee fallen leaves water-leach liquor, it passes through allelopathy, effectively can suppress the growth of Microcystis aeruginosa, thus slow down Growth of Microcystis in Water speed.Specifically, the allelopathy of plant refer to plant pass through to discharge produce in chemical substance to environment to other plant or the direct or indirect illeffects of microorganism, and allelochemical is the non-nutritive substance produced in organism, other plant growth, health, behavior or group relation can be affected, allelochemical is the medium of allelopathy, mainly the secondary metabolites of plant.
In the embodiment of the present invention, the mass concentration of described lichee fallen leaves water-leach liquor is 1.0-3.0 gL
-1.Preferably, the mass concentration of described lichee fallen leaves water-leach liquor is 2.0 gL
-1.The plant defoliation leachate that the present invention only need add low concentration can suppress the growth of Microcystis aeruginosa significantly, and this is that the growth of allelochemical to Microcystis aeruginosa owing to containing in lichee fallen leaves water-leach liquor has very strong inhibitory action.
In addition, described BG-11 medium is formulated by following component: NaNO
31.5g, K
2hPO
40.04g, MgSO
47H
2o 0.075g, CaCl
27H
2o 0.036g, Na
2cO
30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution 1mL, and be settled to 1000 mL with distilled water.In the medium of this formulated, Microcystis aeruginosa can be in good growth conditions, and its time entering exponential phase is short, can grow fast.
Further, the trace element solution in described BG-11 medium is formulated by following component: H
3bO
40.286g, MnCl
24H
2o 0.181g, ZnSO
40.0222g, Na
2moO
40.039g, CuSO
45H
2o 0.0079g, Co (NO
3)
26H
2o 0.00494g, and be settled to 100 mL with distilled water.Trace element solution can provide element needed for growth for Microcystis aeruginosa, also can according to the component of actual conditions adjustment trace element and proportioning thereof, to adapt to the Growth of Microcystis in Water of different growing stages.
Preferably, in described Microcystis aeruginosa culture fluid, after inoculation, the initial density of described Microcystis aeruginosa is 1.0 × 10
5-1.3 × 10
6cellmL
-1.More preferably, after inoculation, the initial density of described Microcystis aeruginosa is about 1.0 × 10
5cellmL
-1.The growth tool of initial density to algae of Microcystis aeruginosa has a certain impact, when the initial density of Microcystis aeruginosa is excessive, nutriment in medium is restricted, the growth of Microcystis aeruginosa can be made to be suppressed, and the initial density of Microcystis aeruginosa too small time, it enters the negligible amounts of the Microcystis aeruginosa of logarithmic phase after cultivating, be unfavorable for the observation of testing.And be about 1.0 × 10 in the initial density of Microcystis aeruginosa
5cellmL
-1time, the growth of Microcystis aeruginosa is best.
Wherein, the Light To Dark Ratio in described illumination box is 12h:12h.Periodicity of illumination is the key factor affecting Growth of Microcystis in Water, the growth of Microcystis aeruginosa is all unfavorable for when unglazed photograph or 24 h light, the present invention is under Light To Dark Ratio 12h:12h, the growth rate of Microcystis aeruginosa is the fastest, employing Light To Dark Ratio is the illumination variation of all right simulating natural environment of 12h:12h in addition, and convenient observation is positioned at the upgrowth situation of the Microcystis aeruginosa under natural environment.
In preferred embodiment, the cultivation temperature in described illumination box is 25-28 DEG C.More preferably, the cultivation temperature in illumination box described in the present invention is 26 DEG C.At such a temperature, Growth of Microcystis in Water is fastest, and it enters the shortest time of exponential phase.
Further, the intensity of illumination in described lighting box is 30 μm of ol m
-2s
-1.Intensity of illumination is the important factor of Growth of Microcystis in Water, the growth of different illumination intensity to Microcystis aeruginosa has obvious difference, in certain illumination range, the photosynthesis rate of Microcystis aeruginosa increases with the increase of illumination, but illumination then may destroy too by force the photosynthetic organs of Microcystis aeruginosa, and then suppress the growth of Microcystis aeruginosa.The present invention after deliberation different illumination intensity finds the impact of the growth of Microcystis aeruginosa, and Growth of Microcystis in Water is 30 μm of ol m with carrying out photosynthetic optimum intensity of illumination
-2s
-1, it can guarantee that Microcystis aeruginosa is in best growth conditions.
In present pre-ferred embodiments, the incubation time in described illumination box is that 14-16d(d refers to number of days).Preferably, the incubation time that the present invention adopts is 15d, when arranging control group and processed group, along with the prolongation of incubation time, in control group, the growth of Microcystis aeruginosa reaches exponential phase, and Growth of Microcystis in Water is controlled in processed group, so that the Growth of Microcystis in Water situation before and after lichee fallen leaves water-leach liquor is added in contrast.
The preparation process that the present invention also provides described lichee to fall leaves water-leach liquor, it specifically comprises:
S1, collect lichee fallen leaves and rinse well with water, being positioned in 40 DEG C of baking ovens to dry after 4 ~ 5d and taking out, be cut into segment and rub that to be broken into broken blade stand-by;
S2, being soaked in distilled water by rubbing broken broken blade, being positioned in climatic cabinate with after sealed membrane sealing, and placing 4 ~ 5 d under dark surrounds, through miillpore filter filtration under diminished pressure, obtain lichee fallen leaves leachate.
The method utilizing lichee fallen leaves to suppress Growth of Microcystis in Water of the present invention, it has the following advantages compared to prior art:
1. Microcystis aeruginosa is the one common Microcystis aeruginosa algae kind that wawter bloom occurs, and proves through test, and prepared lichee fallen leaves water-leach liquor is obvious to its growth inhibitory effect, has more actual application value.
2. the lichee fallen leaves collection of water-leach liquor, preparation technology are simple, and equipment needed thereby is conventional equipment, is suitable for the application of domestic and international different water factory.
3. when incubation time reaches 15 d, compare with control group, Growth of Microcystis in Water is restricted, and growth rate is starkly lower than control group.
4. the growth of allelochemical to Microcystis aeruginosa in lichee fallen leaves water-leach liquor has stronger inhibition, and the mass concentration of lichee fallen leaves water-leach liquor is 2.0 g L
-1time (with lichee fallen leaves dry weight basis), can suppress the algae liquid of 100 mL, effect of algae restraint is obvious.
5. utilize lichee to fall leaves and suppress the growth of Microcystis aeruginosa, there is waste recycling, the feature of " turning waste into wealth ".
6., with the Measures compare of other Chemical Inhibition algal growns, only need the lichee fallen leaves water-leach liquor dropping into low concentration, its processing method is easy, and operating procedure is simple.
7. the present invention is without the need to dropping into other chemical reagent, saves the input cost of other chemical reagent, decreases the problem that sludge quantity increases and treating capacity increases caused after adding other chemical reagent.
8. lichee fallen leaves water-leach liquor is pure natural leachate, is easy degradation material, reduces the secondary pollution problem of chemical reagent.
Below in conjunction with embodiment, the present invention is further detailed.
The preparation method of the plant defoliation leachate in following embodiment is: collect lichee fallen leaves, clean with tap water, then at 40 DEG C, use oven drying 4d, becomes fragile to falling leaves, easily rub broken till; Then be cut into 2 cm segments, and it is for subsequent use to rub the blade being broken into about 0.1cm long; Get rub broken after blade 50 g be soaked in the 1L conical flask that 500 mL distilled water are housed, sealed membrane sealing is placed in 26 ± 1 DEG C of artificial constant temperature climate boxs, 4d is placed under dark surrounds, get the fallen leaves of the lichee after immersion water-leach liquor, via hole diameter is the miillpore filter filtration under diminished pressure of 0.22 μm, filter residue is removed, to reduce the impact of other microorganisms; Leachate after filtration is placed in 4 DEG C of Refrigerator stores, and obtain prepared lichee fallen leaves water-leach liquor, its concentration is 0.1 gmL
-1, color is pale red.
In various embodiments of the present invention, the microcystic aeruginosa be inoculated in medium is in exponential phase, and it is the microcystic aeruginosa liquid that inoculation starts to start to obtain for about 15 days, and color is bottle green.Microcystic aeruginosa described in the present invention is purchased from the American Type Culture Collection committee of Chinese Academy of Sciences algae kind storehouse (FACHB).
Microcystic aeruginosa relies on photosynthesis growth, and chlorophyll a is the main photosynthetic pigment of microcystic aeruginosa, can be used as a kind of index weighing photosynthesis potentiality.As the pigment of live body frustule, the change of leaf green a concentration also reflects the change of Growth of Microcystis aeruginosa in water system.
Embodiment 1
Respectively 100mL BG-11 medium is added in two 250mL conical flasks, and is labeled as No. 1 and No. 2.The 1 mL microcystic aeruginosa liquid being in the logarithmic growth later stage is inoculated in above-mentioned being equipped with in No. 1 of 100 mL BG-11 medium and No. 2 conical flasks, after inoculation, makes the initial density of microcystic aeruginosa be about 1.0 × 10
5cellmL
-1, wherein No. 1 as a control group; No. 2 as processed group.Add the lichee prepared and fall leaves water-leach liquor in medium, after adding, lichee fallen leaves water-leach liquor concentration is 2.0 gL
-1.No. 1 and No. 2 conical flasks are placed in illumination box cultivate, intensity of illumination is about 30 μm of ol m
-2s
-1, Light To Dark Ratio is 12 h:12 h, and temperature is 26 DEG C, and every day, manually timing was shaken 2 times, and incubation time is 15 d.Phytoplankton fluorescence classifying instrument equipment (PHOTO-PAM, German WLAZ company) is adopted to measure the chlorophyll-a concentration of microcystic aeruginosa.Control group and processed group arrange 3 Duplicate Samples respectively, experiment repetition 3 times.
Result shows, at the 1st d, no matter be control group or processed group, its chlorophyll a is about 30 μ gL
-1, the two is more or less the same; When the 15th d, in No. 1 control group, Growth of Microcystis aeruginosa is normal, and its chlorophyll-a concentration is about: 3999 μ gL
-1; And in No. 2 processed group, slowly, its chlorophyll-a concentration is about Growth of Microcystis aeruginosa: 858 μ gL
-1, the growth of visible microcystic aeruginosa is suppressed, and growth rate is necessarily controlled, i.e. the allelochemical of lichee fallen leaves water-leach liquor release effectively can suppress the growth of microcystic aeruginosa.
Embodiment 2
Respectively 100 mL BG-11 medium are added in five 250 mL conical flasks, and are labeled as No. a, No. b, No. c, No. d, No. e and No. f.The 1 mL microcystic aeruginosa liquid being in exponential phase is inoculated in the conical flask that 100 mL BG-11 medium are housed, after inoculation, makes the initial density of microcystic aeruginosa be about 1.0 × 10
5cellmL
-1.Add the lichee prepared and fall leaves water-leach liquor in medium, after adding, lichee fallen leaves water-leach liquor concentration in No. a, No. b, No. c, No. d, No. e and f conical flask is respectively 0.0 gL
-1, 0.4 gL
-1, 0.8 gL
-1, 1.2 gL
-1, 1.6 gL
-1, 2.0 gL
-1.Above-mentioned conical flask is placed in illumination box cultivate, intensity of illumination is about 30 μm of ol m
-2s
-1, Light To Dark Ratio is 12h:12h, and temperature is 28 DEG C, and every day manually shakes 2 times, and incubation time is 15 d.Adopt phytoplankton fluorescence classifying instrument measuring apparatus algae chlorophyll-a concentration.Often organize and 3 Duplicate Samples are set respectively, experiment repetition 3 times.
Result shows, lichee fallen leaves water-leach liquor concentration is 0.0 gL
-1no. a group Growth of Microcystis aeruginosa normal, when 15d, its chlorophyll-a concentration is about: 5303 μ gL
-1; Concentration is 0.4 gL
-1b group Growth of Microcystis aeruginosa speed do not have No. a to organize fast, the growth of microcystic aeruginosa is subject to certain inhibitory action, and when 15d, its chlorophyll-a concentration is about: 4215 μ gL
-1; Concentration is 0.8 gL
-1c group Growth of Microcystis aeruginosa speed do not have No. b to organize fast yet, the growth of microcystic aeruginosa is subject to stronger inhibitory action, and 15d chlorophyll-a concentration is about: 2985 μ gL
-1; Concentration is 1.2 gL
-1d group Growth of Microcystis aeruginosa speed do not have No. c to organize fast yet, the growth of microcystic aeruginosa is subject to more obvious inhibitory action, and when 15d, its chlorophyll-a concentration is about: 2075 μ gL
-1; Concentration is 1.6 gL
-1e group Growth of Microcystis aeruginosa speed do not have No. d to organize fast yet, the growth of microcystic aeruginosa is subject to more obvious inhibitory action, and when 15d, its chlorophyll-a concentration is about: 2409 μ gL
-1; Concentration is 2.0 gL
-1f group Growth of Microcystis aeruginosa speed the slowest, when 15d, its chlorophyll-a concentration is: 845 μ gL
-1.Visible, (2.0 gL when the lichee fallen leaves water-leach liquor of high concentration
-1), its inhibition to Growth of Microcystis aeruginosa is best.Experiment also finds, thereupon the increasing of concentration, and can increase the risk of water nutrition, therefore adopt 2.0 gL
-1best inhibition and water nutrition balance can be reached.
Embodiment 3
Respectively 100mL BG-11 medium is added in two 250 mL conical flasks, and is labeled as No. A1 and No. A2.The 1 mL microcystic aeruginosa liquid being in the logarithmic growth later stage is inoculated in No. A1 and A2 conical flask that 100 mL BG-11 medium are housed, after inoculation, makes the initial density of microcystic aeruginosa be about 1.0 × 10
5cellmL
-1.No. A1 as a control group, and No. A2, as processed group, adds the lichee prepared and fall leaves water-leach liquor in medium, and after adding, lichee fallen leaves water-leach liquor concentration is 2.0 gL
-1.No. A1 and A2 conical flask are placed in illumination box cultivate, intensity of illumination is 30 μm of ol m
-2s
-1, Light To Dark Ratio is 12h:12h, and temperature is 25 DEG C, and every day manually shakes 2 times.A1 control group and A2 processed group are sampled respectively at the 1st, 4,7,10 and 15d, adopts phytoplankton fluorescence classifying instrument measuring apparatus algae chlorophyll-a concentration.Often organize and 3 Duplicate Samples are set respectively, experiment repetition 3 times.
Result shows, along with the prolongation of incubation time, in A1 control group, the chlorophyll a of microcystic aeruginosa is in the trend significantly risen, and Growth of Microcystis in Water is normal, and the chlorophyll a of the 1st, 4,7,10 and 15 d is about respectively: 22 μ gL
-1, 42 μ gL
-1, 139 μ gL
-1, 1273 μ gL
-1, 4365 μ gL
-1; In A2 processed group, the chlorophyll a of microcystic aeruginosa is remarkable downward trend, and the growth of microcystic aeruginosa is subject to suppression in various degree.In A1 processed group, Growth of Microcystis in Water is suppressed, and the chlorophyll a of the 1st, 4,7,10 and 15 d is about respectively: 24 μ gL
-1, 16 μ gL
-1, 42 μ gL
-1, 215 μ gL
-1, 1197 μ gL
-1.Visible, adding concentration is 2.0 gL
-1lichee fallen leaves water-leach liquor time, when normal incubation time is 15 d, its chlorophyll-a concentration is about 27% of control group, effectively slow down Growth of Microcystis in Water speed.
Known by above embodiment, lichee fallen leaves water-leach liquor can effectively suppress Growth of Microcystis in Water speed, and in actual applications, the allelochemical that lichee fallen leaves water-leach liquor contains is utilized to control Microcystis aeruginosa, other any chemical reagent need not be added, it can play certain inhibitory action to the growth of Microcystis aeruginosa, for the wawter bloom controlling Microcystis aeruginosa.The present invention is simple to operate, processing cost is low and energy twice laid, achieves the control to fresh water water system typical case algae excessive propagation, ensure that the safety of fresh water water system environment, for control microcystis waterbloom, has broad application prospects.
Be understandable that, for those of ordinary skills, can be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, and all these change or replace the protection domain that all should belong to the claim appended by the present invention.
Claims (10)
1. utilize lichee to fall leaves and suppress a method for Growth of Microcystis in Water, it is characterized in that, comprise the following steps:
A, the lichee fallen leaves after cleaning are carried out drying, rub broken process, and soak with distilled water, prepare lichee fallen leaves water-leach liquor stand-by;
B, the Microcystis aeruginosa being in exponential phase is inoculated in BG-11 medium, preparation Microcystis aeruginosa culture fluid;
C, in above-mentioned prepared Microcystis aeruginosa culture fluid, add lichee fallen leaves water-leach liquor, and be placed in illumination box and cultivate with illumination and the dark mode replaced, suppressed the growth of Microcystis aeruginosa by lichee fallen leaves water-leach liquor.
2. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step, the mass concentration of described lichee fallen leaves water-leach liquor is 1.0-3.0 gL
-1.
3. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in stepb, described BG-11 medium is formulated by following component: NaNO
31.5g, K
2hPO
40.04g, MgSO
47H
2o 0.075g, CaCl
27H
2o 0.036g, Na
2cO
30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution 1mL, and be settled to 1000 mL with distilled water.
4. utilize lichee to fall leaves according to claim 3 and suppress the method for Growth of Microcystis in Water, it is characterized in that, described trace element solution is formulated by following component: H
3bO
40.286g, MnCl
24H
2o 0.181g, ZnSO
40.0222g, Na
2moO
40.039g, CuSO
45H
2o 0.0079g, Co (NO
3)
26H
2o 0.00494g, and be settled to 100 mL with distilled water.
5. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in stepb, in described Microcystis aeruginosa culture fluid, the initial density of Microcystis aeruginosa is 1.0 × 10
5-1.3 × 10
6cellmL
-1.
6. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step C, the Light To Dark Ratio in described illumination box is 12h:12h.
7. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step C, the cultivation temperature in described illumination box is 25-28 DEG C.
8. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step C, the intensity of illumination in described lighting box is 30 μm of ol m
-2s
-1.
9. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step C, the incubation time in described illumination box is 14-16d.
10. utilize lichee to fall leaves according to claim 1 and suppress the method for Growth of Microcystis in Water, it is characterized in that, in step, the concrete preparation process of described lichee fallen leaves water-leach liquor is:
S1, collect lichee fallen leaves and rinse well with water, being positioned in 40 DEG C of baking ovens to dry after 4 ~ 5 d and taking out, being rubbed that to be broken into broken blade stand-by;
S2, broken blade is soaked in distilled water, is positioned in climatic cabinate with after sealed membrane sealing, and places 4 ~ 5 d under dark surrounds, through miillpore filter filtration under diminished pressure, obtain lichee fallen leaves water-leach liquor.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136129A (en) * | 2017-06-08 | 2017-09-08 | 合肥龙滨化工科技有限公司 | A kind of plant pesticide and preparation method thereof |
| CN113603195A (en) * | 2021-07-30 | 2021-11-05 | 深圳职业技术学院 | Algae inhibitor and preparation method thereof |
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| Title |
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| 上官小亚，洪喻: "白蜡落叶酸化预处理及酸化液抑藻活性的研究", 《2013北京国际环境技术研讨会论文集》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136129A (en) * | 2017-06-08 | 2017-09-08 | 合肥龙滨化工科技有限公司 | A kind of plant pesticide and preparation method thereof |
| CN113603195A (en) * | 2021-07-30 | 2021-11-05 | 深圳职业技术学院 | Algae inhibitor and preparation method thereof |
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