CN107460128A - A kind of method using biogas slurry cultivating microalgae - Google Patents

A kind of method using biogas slurry cultivating microalgae Download PDF

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CN107460128A
CN107460128A CN201710681636.0A CN201710681636A CN107460128A CN 107460128 A CN107460128 A CN 107460128A CN 201710681636 A CN201710681636 A CN 201710681636A CN 107460128 A CN107460128 A CN 107460128A
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biogas slurry
microalgae
growth rate
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CN107460128B (en
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国青青
刘锐
王根荣
陈吕军
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Yangtze Delta Region Institute of Tsinghua University Zhejiang
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Yangtze Delta Region Institute of Tsinghua University Zhejiang
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention discloses a kind of method using biogas slurry cultivating microalgae, microalgae is seeded in synthetic medium, harvested when the specific growth rate of microalgae is begun to decline, then biogas slurry is added in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate 60~70%, harvested；Add biogas slurry in batches again, treat microalgae growth rate be down to first add biogas slurry before growth rate 50~60%, harvested；Third time add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate less than 50%, after being harvested terminate cultivation.The present invention proposes a kind of rational detailed method of operation that cultivating microalgae is realized using biogas slurry, improves the yield of microalgae, reduces both culturing microalgae cost, farthest improves the gain effect that both culturing microalgae is combined with other techniques.

Description

A kind of method using biogas slurry cultivating microalgae
Technical field
The present invention relates to field of microalgae cultivation and field of waste water treatment, and in particular to a kind of side using biogas slurry cultivating microalgae Method.
Background technology
Domestic live pig consumption figure accounts for the 20% of world's aggregate consumption, and huge pig-breeding market generates substantial amounts of support Pig biogas slurry.Pig biogas slurry belongs to high concentrated organic wastewater, the characteristic with " high COD, high SS, high nitrogen phosphorus ", such as without processing Surrounding water environment will seriously be polluted.On the other hand, biogas slurry caused by livestock breeding wastewater anaerobic fermentation, except containing abundant Outside the Meso- and micro-nutrients such as the macronutrients such as nitrogen, phosphorus, potassium and calcium, copper, iron, zinc, manganese, also containing abundant amino acid, B Family vitamin, various hydrolases, certain plants hormone and the material or the factor that have inhibitory action to pest and disease damage, it is a kind of precious Renewable resource, it is significant to its recycling.
Microalgae needs to consume the inorganic matter nutritive salt such as substantial amounts of nitrogen phosphorus in growth course, and contains in biogas slurry of raising pigs simultaneously Substantial amounts of nutritive salt can not be removed.Spirulina is as the outstanding person in microalgae, because of its economic value height, strong stress resistance, Yi Cai The features such as receipts the emphasis by scientists as waste water culture experiment.Have document to show to utilize biogas slurry culture spirulina, be Inorganic matter in biogas slurry is converted into the process of organic matter using solar energy, not only sewage is turned waste into wealth, is imitated in generation environment Certain economic benefit is generated while beneficial.Therefore, there are wide market prospects and actual meaning using biogas slurry cultivating microalgae Justice.
Such as Li Yubao (utilizes methane waste liquor culture spirulina, Li Yubao, Zheng Jiang, Li Zhongbao, Lu Bin etc., Agriculture of Anhui Science, 2011,39 (22):13668-13670) by adding certain density Zarrouk nutrient solutions into biogas slurry, spiral shell is obtained Culture technique of the algae in biogas slurry is revolved, and have studied clean-up effect of the spirulina to biogas slurry.And for example, Publication No. CN A kind of precipitated using guanite is disclosed in 103819053 A Chinese patent literature and couples microdisk electrode progress biogas slurry and biogas The method of purification；One kind is disclosed in the A of Publication No. CN 102392052 Chinese patent literature and utilizes biogas slurry culture autotrophic type The method of freshwater microalgae purifying biogas.
Understand, be placed on purification and the money of biogas slurry for how realizing biogas slurry more the emphasis that biogas slurry is used for microdisk electrode at present Sourceization utilizes upper.It is not directed to the specific detailed protocol for how utilizing waste water cultivating microalgae.But waste water especially biogas slurry is one The waste water of kind complicated component, also still there is the growth of many residues suppression microalgaes even across pretreatment, microalgae can not be realized Cultivate maximum gain effect.
The content of the invention
The present invention proposes a kind of rational detailed method of operation that cultivating microalgae is realized using biogas slurry, improves the production of microalgae Amount, both culturing microalgae cost is reduced, farthest improve the gain effect that both culturing microalgae is combined with other techniques.
Concrete technical scheme is as follows：
A kind of method using biogas slurry cultivating microalgae, step are as follows：
(1) microalgae is seeded in synthetic medium, harvested when the specific growth rate of microalgae is begun to decline, then Add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate 60~70%, harvested；
(2) add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate 50~ 60%, harvested；
(3) add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate 50% with Under, terminate cultivation after being harvested.
Growing state of the invention according to microalgae, microdisk electrode is divided into domestication stage, the mid-term microalgae height of microalgae at initial stage Production stage and later stage both culturing microalgae ending phase.Pretreated biogas slurry is added in batches in each stage, and accurately Control biogas slurry adds opportunity and the addition per the stage, per batch biogas slurry, farthest improve both culturing microalgae with it is other The gain effect that technique combines.
Specific growth rate μ in step (1) refers to：The increased biomass of thalline institute of unit mass per hour, formula is such as Under：
lnNt-lnN0=μ t
In formula, μ-specific growth rate, unit h-1；T- times, unit h；N0Microbial cell amount during-beginning, Nt- start to train Biological cell amount after the foster t times.
Growth rate in step (1)~(3) is absolute growth rate, when referring to utilize synthetic medium culture microalgae Average growth rate.
The domestication stage of microalgae at initial stage is also divided into pure culture stage and biogas slurry cultivation stage.Wherein, the pure culture stage is Only with synthetic medium culture microalgae, the stage that biogas slurry is cultivated is not added.Biogas slurry cultivation stage using add first biogas slurry as Start, the mark of end is that the growth rate of microalgae is down to add first before biogas slurry 60~70%.
Initial stage microalgae the domestication stage terminate after enter the mid-term microalgae high yield stage, the mid-term microalgae high yield stage terminates Mark is that the growth rate of microalgae is down to 50~60% added first before biogas slurry.
The mid-term microalgae high yield stage enters later stage both culturing microalgae ending phase after terminating, and the mark that the stage terminates is The growth rate of microalgae is down to less than 50% added first before biogas slurry.
Preferably, described synthetic medium composition is as follows：
NaCl 1g/L, CaCl20.041g/L, NaNO32.51g/L FeSO4·7H2O 0.011g/L, EDTA-Na 0.081g/L, K2SO4 1.01g/L, NaHCO316.81g/L K2HPO40.51g/L, MgSO4·7H2O 0.251g/L。
Because of the complicated component of biogas slurry, wherein the impurity containing suppression micro algae growth, therefore Biological Pretreatment need to be carried out.Institute The Biological Pretreatment stated includes aerobe reaction, is such as pre-processed using MBR reactors.
Preferably, ammonia nitrogen is less than 200mg/L in biogas slurry effluent quality after Biological Pretreatment, COD is less than 500mg/ L, to prevent biogas slurry from suppressing the growth of microalgae.
Preferably, described microalgae is selected from chlorella or spirulina.
Preferably, in step (1)~(3), described biogas slurry is independently divided into the addition of 1~4 batch, and adjacent two batch adds On the opportunity entered, start to reduce as timing node using the specific growth rate of microalgae, the biogas slurry amount added per batch fills for culture It is set effective the 10~50% of volume.
The form of ammonia nitrogen in the solution is temperature dependent with pH, and when temperature is higher and pH is more than 9.25, it is mainly with trip Form from ammonia is present, and free ammonia is poisonous to photosynthetic organism, and the concentration of the biogas slurry amount of addition and ammonia nitrogen in culturing pool is just Correlation, as preferred：
In step (1), total biogas slurry amount of addition need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 20mg/L；
In step (2), total biogas slurry amount of addition need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 40mg/L；
In step (3), total biogas slurry amount of addition need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 60mg/L.
Preferably, in step (1), the biogas slurry amount added per batch is identical；
In step (2), the biogas slurry amount added per batch is identical；
In step (3), the biogas slurry amount added per batch is identical.
Further preferably, in step (2), the biogas slurry amount added per batch is to add the 1 of biogas slurry amount in step (1) per batch ~4 times；
In step (3), the biogas slurry amount added per batch is 1~4 times that adds biogas slurry amount in step (1) per batch.
Preferably, in step (1)~(3), first harvested before adding biogas slurry every time, then carry out draining, each draining Amount is equal to each biogas slurry addition with water evaporation quantity sum.
In both culturing microalgae, after each harvesting, water sample is taken in culturing pool to detect water quality situation, and according to circumstances mend in time Fill nutritive salt.
It can be seen from experimental data, when the microalgae of culture is selected from chlorella, most preferably：
In step (1), initial stage microalgae the domestication stage, supplement a biogas slurry, the biogas slurry amount of addition is effective for culture apparatus The 20% of volume；
In step (2), in the mid-term microalgae high yield stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch fills for culture It is set effective the 40% of volume；
In step (3), later stage both culturing microalgae ending phase, biogas slurry is supplemented at twice, the biogas slurry amount added per batch is training Support the 30% of device dischargeable capacity.
When the microalgae of culture is selected from spirulina, most preferably：
In step (1), initial stage microalgae the domestication stage, supplement a biogas slurry, the biogas slurry amount of addition is total water in culturing pool The 10% of amount；
In step (2), in the mid-term microalgae high yield stage, biogas slurry is supplemented in four times, the biogas slurry amount added per batch is culturing pool The 30% of interior total Water；
In step (3), later stage both culturing microalgae ending phase, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is foster Grow 40% of total Water in pond.
Compared with prior art, the present invention has advantages below：
(1) batch addition biogas slurry is advantageous to the water stabilization of micro-algae culture pond in this method, reduces the water quality because of biogas slurry The risk being greatly reduced with Quality Down of micro algae growth caused by fluctuation.It is always the advantage group in culturing pool to make target algae kind Body, reduce contaminated risk.The continuous incubation time of microalgae is extended simultaneously, microalgae yield is improved and utilizing for waste water is imitated Rate, significantly reduce production cost.
(2) stage culture realizes the domestication of algae kind and the production of algae kind in same culturing pool in this method, reduces hardware and sets The outfit applied, valuable time cost is more saved.
(3) biogas slurry adding method and water discharge method stage in this method, it both ensure that microalgae nutrition was filled in culturing pool Foot, while mortifier can be also excluded in time, improve the speed of growth of microalgae.
Brief description of the drawings
Fig. 1~6 are respectively the growth curve of chlorella in comparative example 1~2 and embodiment 1~4；
Fig. 7~12 are respectively the growth curve of spirulina in comparative example 4 and embodiment 5~9.
Embodiment
The component and condition of culture in biogas source, synthetic medium in embodiment 1~4 and comparative example 1~2 are as follows：
Biogas slurry source：Certain livestock and poultry farm, cultivation scale are 3000 first-born pig of livestock on hand, pig manure and biogas slurry separating dry space from moist space, pig Excrement is handled through compost fermentation and is prepared into organic fertilizer, and biogas slurry is discharged after entering methane-generating pit anaerobic digestion, aerobe method processing.Biogas slurry Effluent quality：COD 257mg/L, ammonia nitrogen 86mg/L, total nitrogen 429mg/L, total phosphorus 16.7mg/L.In following examples, such as without spy Different explanation, biogas slurry refer both to the biogas slurry after Biological Pretreatment.
Synthetic medium：Culture medium is the BG11 culture mediums of improvement, and composition is as follows：NaNO30.5g/L, K2HPO4·3H2O 0.1g/L, CaCl2·2H2O 0.0025g/L, MgSO4·7H2O 0.075g/L；Citric acid 0.006g/L, ironic citrate 0.006g/L, Na2CO30.02g/L, micro- mother liquor 1mL/L.Micro- mother liquor formula (g/L):H3BO42.86g/L MnCl2﹒ 4H2O 1.81g/L, ZnSO40.222g/L, Na2MoO40.39g/L, CuSO4﹒ 5H2O0.079g/L, Co (NO3)2﹒ 6H2O 49.4g/L。
Condition of culture：The long high respectively flat Photoreactors of 50cm*20cm*40cm of the wide * of *, dischargeable capacity 25L.It is outdoor Culture, 22-35 DEG C of temperature, is continuously passed through air 12h, throughput 1.5L/min daytime.Supplement sodium carbonate regulation pH to 7-9.
Comparative example 1：
2g/L sodium carbonate regulation pH is added in biogas slurry.Using 100% biogas slurry culture chlorella, average product is 0.027g/L/d, growth curve such as Fig. 1 of chlorella.
Comparative example 2
Initial medium is synthetic medium 90% and biogas slurry 10%, average product 0.17g/L/d.9th day, the 15th My god, the 20th day respectively supplement 2.5L biogas slurries, average product 0.19g/L/d.26th day supplement 2.5L biogas slurry, average product are 0.05/L/d.The growth curve of chlorella such as Fig. 2.
Embodiment 1
Initial medium is synthetic medium.6th day supplement 2.5L biogas slurry, not draining, average product 0.17g/L/d, That is the domestication stage of microalgae at initial stage, a biogas slurry is supplemented, the biogas slurry amount of addition is the 10% of culture apparatus dischargeable capacity.11st My god, supplement 5L biogas slurries respectively within the 16th day, the 21st day, while draining 2.5L, average product 0.19g/L/d, i.e. mid-term microalgae are high In the production stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is the 20% of culture apparatus dischargeable capacity.Supplement within 25th day 10L biogas slurries, while draining 10L, average product 0.17g/L/d, i.e. later stage both culturing microalgae ending phase, a biogas slurry is supplemented, The biogas slurry amount of addition is the 40% of culture apparatus dischargeable capacity.The growth curve of chlorella such as Fig. 3.
Embodiment 2
Initial medium is synthetic medium.6th day supplement 5L biogas slurry, draining 2.5L, average product 0.4g/L/d, i.e., Initial stage microalgae the domestication stage, supplement a biogas slurry, the biogas slurry amount of addition is the 20% of culture apparatus dischargeable capacity.10th day, 10L biogas slurries, while draining 7.5L, average product 0.31g/L/d, i.e. mid-term microalgae high yield are supplemented respectively within 19th day, the 24th day In the stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is the 40% of culture apparatus dischargeable capacity.27th day supplement 15L Biogas slurry, while draining 15L, average product 0.07g/L/d, i.e. later stage both culturing microalgae ending phase, a biogas slurry is supplemented, added Biogas slurry amount be culture apparatus dischargeable capacity 60%.The growth curve of chlorella such as Fig. 4.
Embodiment 3
Initial medium is synthetic medium.6th day supplement 7.5L biogas slurry, draining 5L, average product 0.28g/L/d, That is the domestication stage of microalgae at initial stage, a biogas slurry is supplemented, the biogas slurry amount of addition is the 30% of culture apparatus dischargeable capacity.13rd My god, supplement 15L biogas slurries, while draining 12.5L, average product 0.21g/L/d, i.e. mid-term microalgae high yield rank respectively within the 20th day Section, supplements biogas slurry at twice, and the biogas slurry amount added per batch is the 60% of culture apparatus dischargeable capacity.20th day, the 25th natural gift Not Bu Chong 7.5L biogas slurries, while draining 7.5L, average product 0.22g/L/d, i.e. later stage both culturing microalgae ending phase, at twice Biogas slurry is supplemented, the biogas slurry amount added per batch is the 30% of culture apparatus dischargeable capacity.The growth curve of chlorella such as Fig. 5.
Embodiment 4
Initial medium is synthetic medium.6th day supplement 5L biogas slurry, draining 2.5L, average product 0.4g/L/d, i.e., Initial stage microalgae the domestication stage, supplement a biogas slurry, the biogas slurry amount of addition is the 20% of culture apparatus dischargeable capacity.10th day, 10L biogas slurries, while draining 7.5L, average product 0.31g/L/d, i.e. mid-term microalgae high yield are supplemented respectively within 19th day, the 24th day In the stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is the 40% of culture apparatus dischargeable capacity.20th day, the 25th day 7.5L biogas slurries, while draining 7.5L, average product 0.22g/L/d, i.e. later stage both culturing microalgae ending phase are supplemented respectively, are divided to two Secondary supplement biogas slurry, the biogas slurry amount added per batch are the 30% of culture apparatus dischargeable capacity.The growth curve of chlorella such as Fig. 6.
The component and condition of culture in biogas source, synthetic medium in embodiment 5~9 and comparative example 3~4 are as follows：
Biogas slurry source：Certain livestock and poultry farm, cultivation scale are 3000 first-born pig of livestock on hand, pig manure and biogas slurry separating dry space from moist space, pig Excrement is handled through compost fermentation and is prepared into organic fertilizer, and biogas slurry is discharged after entering methane-generating pit anaerobic digestion, aerobe method processing.Biogas slurry Effluent quality：COD 250-450mg/L, ammonia nitrogen 100-200mg/L, total nitrogen 400-800mg/L, total phosphorus 20-40mg/L.
Synthetic medium is the Zarrouk culture mediums of improvement, and composition is as follows：NaCl 1g/L, NaNO31g/L, FeSO4· 7H2O 0.08g/L, EDTA-Na 0.01g/L, K2SO4 1g/L, NaHCO310g/L, K2HPO4 0.1g/L。
Culturing pool：40 square meters (long 10 meters, wide 4 meters) elliptic chamber outer track pond, daytime is stirred using 6 impeller agitating paddles Continuously stir 12h, mean depth 10cm, 4 tons of water.Water temperature is at 25-38 DEG C.Regular replenishment sodium bicarbonate is as carbon source.
Comparative example 3
The culture of spirulina is directly used in after biogas slurry addition sodium bicarbonate 10g/L, growth of spirulina platensis is slow, all becomes within the 3rd day It is yellow dead.
Comparative example 4
Synthetic medium and 10% biogas slurry that culture medium is 90%, the 23rd day 0.8 ton of biogas slurry of supplement, discovery in the 15th day have A large amount of red pest pollutions, controlled after adding insecticide in time, average product is about 2g/m2/d.Growth curve such as Fig. 7.
Embodiment 5
Initial medium is synthetic medium, and the 7th day 0.4 ton of biogas slurry of addition, not draining, average product is about 14.2g/ m2In/d, i.e. microalgae at initial stage the domestication stage, a biogas slurry is supplemented, the biogas slurry amount of addition is total Water (i.e. culturing pool in culturing pool Dischargeable capacity) 10%.Add 0.8 ton of biogas slurry, while ton/time of draining 0.4, average product within 13rd day, the 20th day, the 24th day About 11.7g/m2/ d, i.e. in the mid-term microalgae high yield stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is in culturing pool The 20% of total Water.33rd day, the 40th day, 1.2 tons of biogas slurry is added, while ton/time of draining 1.2, average product are about 10g/ m2/ d, i.e. later stage both culturing microalgae ending phase, supplement biogas slurry at twice, and the biogas slurry amount added per batch is total Water in culturing pool 30%；Harvest 1 time respectively within 7th day, the 12nd day, the 19th day, the 23rd day, the 32nd day, the 39th day；Terminate culture within 44th day.It is raw Long curve such as Fig. 8.
Embodiment 6
Initial medium is synthetic medium, the 7th day 0.8 ton of biogas slurry of addition, 0.4 ton of draining, and average product is about 12.3g/m2In/d, i.e. microalgae at initial stage the domestication stage, a biogas slurry is supplemented, the biogas slurry amount of addition is total Water in culturing pool 20%；1.6 tons of biogas slurry is added respectively within 12nd day, the 19th day, ton/time of draining 1.2, average product is about 7.9g/m2/ d, i.e. mid-term In the microalgae high yield stage, biogas slurry is supplemented at twice, the biogas slurry amount added per batch is 40% of total Water in culturing pool；33rd day, 3.2 tons of biogas slurry is added respectively within 40th day, ton/time of draining 2.8, average product is about 4.1g/m2/ d, i.e. later stage both culturing microalgae terminate In the stage, biogas slurry is supplemented at twice, the biogas slurry amount added per batch is 80% of total Water in culturing pool.10th day, the 18th day, Harvested respectively within 31 days, the 39th day.Growth curve such as Fig. 9.
Embodiment 7
Initial medium is synthetic medium, and the 6th day 0.4 ton of biogas slurry of addition, not draining, average product is about 15g/m2/ In d, i.e. microalgae at initial stage the domestication stage, a biogas slurry is supplemented, the biogas slurry amount of addition is 10% of total Water in culturing pool；11st day 1.6 tons of biogas slurry is added, 1.2 tons of draining, average product is about 7.5g/m2/ d, i.e. mid-term microalgae high yield stage, supplement a natural pond Liquid, the biogas slurry amount of addition is 40% of total Water in culturing pool；19th day, the 31st day respectively add 2.4 tons of biogas slurry, 2 tons of draining, Average product is about 5.5g/m2/ d, i.e. later stage both culturing microalgae ending phase, supplement biogas slurry at twice, the biogas slurry added per batch Measure as 60% of total Water in culturing pool.Harvested respectively within 5th day, the 10th day, the 18th day, the 30th day.Growth curve is such as Figure 10.
Embodiment 8
Initial medium is synthetic medium, and the 6th day 0.4 ton of biogas slurry of addition, not draining, average product is about 13.6g/ m2In/d, i.e. microalgae at initial stage the domestication stage, a biogas slurry is supplemented, the biogas slurry amount of addition is 10% of total Water in culturing pool.The 0.4 ton of biogas slurry is added respectively within 12 days, the 17th day, the 21st day, not draining, average product is about 8.1g/m2/ d, i.e. mid-term microalgae are high In the production stage, biogas slurry is supplemented in three times, the biogas slurry amount added per batch is 10% of total Water in culturing pool.28th day, the 33rd day, 0.4 ton of biogas slurry is added respectively within 39th day, not draining, average product is about 5g/m2/ d, i.e. later stage both culturing microalgae ending phase, point Biogas slurry is supplemented three times, and the biogas slurry amount added per batch is 10% of total Water in culturing pool.5th day, the 11st day, the 16th day, Harvested respectively within 20 days, the 27th day, the 32nd day, the 38th day.Average product is 9.5g/m2/d.Growth curve such as Figure 11.
Embodiment 9
Initial medium is synthetic medium, and the 4th day 0.4 ton of biogas slurry of addition, not draining, average product is about 16g/m2/ In d, i.e. microalgae at initial stage the domestication stage, a biogas slurry is supplemented, the biogas slurry amount of addition is 10% of total Water in culturing pool.11st My god, add 1.2 tons of biogas slurry within the 16th day, the 21st day, the 26th day, while ton/time of draining 0.8, average product are about 11.9g/m2/ D, i.e. in the mid-term microalgae high yield stage, biogas slurry is supplemented in four times, the biogas slurry amount added per batch is 30% of total Water in culturing pool. Add 1.6 tons of biogas slurry within 33rd day, the 39th day, the 44th day, 1.6 tons of draining every time, average product about 10g/m2/ d, i.e. later stage Both culturing microalgae ending phase, supplements biogas slurry in three times, and the biogas slurry amount added per batch is 40% of total Water in culturing pool；4th My god, the 10th day, the 15th day, the 20th day, the 25th day, the 32nd day, the 38th day, the 43rd day respectively harvest 1 time；Terminate training within 49th day Support.Growth curve such as Figure 12.

Claims (8)

  1. A kind of 1. method using biogas slurry cultivating microalgae, it is characterised in that step is as follows：
    (1) microalgae is seeded in synthetic medium, harvested when the specific growth rate of microalgae is begun to decline, then in batches Secondary addition biogas slurry, treat microalgae growth rate be down to first add biogas slurry before growth rate 60~70%, harvested；
    (2) add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate 50~60%, enter Row harvesting；
    (3) add biogas slurry in batches, treat microalgae growth rate be down to first add biogas slurry before growth rate less than 50%, enter Terminate cultivation after row harvesting.
  2. 2. the method according to claim 1 using biogas slurry cultivating microalgae, it is characterised in that described biogas slurry need to pass through life Thing pre-processes, and ammonia nitrogen is less than 200mg/L in its effluent quality, and COD is less than 500mg/L.
  3. 3. the method according to claim 1 using biogas slurry cultivating microalgae, it is characterised in that described microalgae is selected from bead Algae or spirulina.
  4. 4. the method according to claim 1 using biogas slurry cultivating microalgae, it is characterised in that in step (1)~(3), institute The biogas slurry stated independently be divided into 1~4 batch addition, per batch add biogas slurry amount for culture apparatus dischargeable capacity 10~ 50%.
  5. 5. the method according to claim 5 using biogas slurry cultivating microalgae, it is characterised in that in step (1), addition it is total Biogas slurry amount need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 20mg/L；
    In step (2), total biogas slurry amount of addition need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 40mg/L；
    In step (3), total biogas slurry amount of addition need to ensure that the concentration of ammonia nitrogen in culturing pool is no more than 60mg/L.
  6. 6. the method according to claim 5 using biogas slurry cultivating microalgae, it is characterised in that in step (1), add per batch The biogas slurry amount entered is identical；
    In step (2), the biogas slurry amount added per batch is identical；
    In step (3), the biogas slurry amount added per batch is identical.
  7. 7. the method according to claim 6 using biogas slurry cultivating microalgae, it is characterised in that in step (2), add per batch The biogas slurry amount entered is add biogas slurry amount in step (1) per batch 1~4 times；
    In step (3), the biogas slurry amount added per batch is 1~4 times that adds biogas slurry amount in step (1) per batch.
  8. 8. the method according to claim 1 using biogas slurry cultivating microalgae, it is characterised in that in step (1)~(3), often First harvested before secondary addition biogas slurry, then carry out draining, each displacement adds with water evaporation quantity sum equal to each biogas slurry Amount.
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