CN103819053A - Biogas slurry and biogas purifying method by struvite precipitation coupled with microalgae cultivation - Google Patents

Biogas slurry and biogas purifying method by struvite precipitation coupled with microalgae cultivation Download PDF

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CN103819053A
CN103819053A CN201410062868.4A CN201410062868A CN103819053A CN 103819053 A CN103819053 A CN 103819053A CN 201410062868 A CN201410062868 A CN 201410062868A CN 103819053 A CN103819053 A CN 103819053A
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liquid
algae
micro
natural pond
biogas
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CN103819053B (en
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王文国
潘科
汤晓玉
祝其丽
胡启春
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Biogas Institute of Ministry of Agriculture
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Biogas Institute of Ministry of Agriculture
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Abstract

The invention discloses a biogas slurry and biogas purifying method by struvite precipitation coupled with microalgae cultivation, comprising the following steps: a, concentrations of NH4<+>, Mg<2+> and PO4<3-> in biogas slurry after anaerobic fermentation of breeding wastewater are measured; b, Mg<2+> and PO4<3-> are added into the biogas slurry to generate struvite precipitate, and the addition amount is based on the standard that removal rate of NH4<+> reaches 70%-90%; c, after struvite precipitation, solids and a liquid are separated naturally, and pH value of the liquid is adjusted within the range suitable for the growth of microalgae so as to promote cultivation of microalgae; d, microalgae is inoculated to the liquid with adjusted pH value and then desulfurized biogas is blown into the liquid for cultivation; and e, solid-liquid separation is carried out after cultivation, and microalgae is collected and utilized. By the struvite precipitation technology, the concentration of ammonia and nitrogen in the biogas slurry is reduced and transparency of the biogas slurry is promoted, thus being beneficial to the growth of microalgae. By the utilization of microalgae, CO2 in the biogas and nutrient salt such as nitrogen and phosphorus in the biogas slurry are removed so as to purify the biogas slurry and the biogas. Therefore, utilization value of biogas can be raised, and biogas engineering operating cost can be reduced.

Description

Utilize the micro-algae of struvite precipitation coupling to cultivate the method for carrying out natural pond liquid and marsh gas purifying
Technical field
The invention belongs to bioenergy and develop and field of environment protection, be specifically related to a kind ofly utilize be coupled micro-algae of struvite crystallization to cultivate the method for carrying out natural pond liquid and marsh gas purifying.
Background technology
Biogas technology is the Main Means that China's livestock and poultry farm carries out offal treatment, recycling, but the problem of environmental pollution of natural pond liquid and the low problem of biogas grade remain the subject matter of restriction biogas industry development.In the liquid of natural pond, still contain the nutrient salts such as a large amount of nitrogen phosphorus, directly discharge can cause environmental pollution, causes surface water body eutrophication, and may impact underground water.Biogas is a kind of mixed gas, except containing methane (CH 4) outside, also contain certain carbonic acid gas (CO 2), steam, nitrogen (N 2) etc. gas, wherein CO 2too high levels can affect suitability and the calorific value of biogas, CO 2removal be the important content of marsh gas purifying.
Mainly contain " also field ", " naturally processing ", " through engineering approaches processing " isotype for the disposal of natural pond liquid at present.Natural pond liquid also field needs a large amount of soils, and is difficult to solve the contradiction between continuity and the seasonality of agricultural use that natural pond liquid produces." naturally process " refers to that methods such as utilizing oxidation pond, artificial swamp, land percolation carries out natural pond liquid processing, although investment economize, less energy consumption, operational and administrative expenses is low, also needs a large amount of soils, is subject to the impact in season, and has the risk of polluted underground water." through engineering approaches processing " refers to that physics, chemistry or biological means such as utilizing aeration, stripping, ion-exchange, membrane filtration carry out processing up to standard to natural pond liquid, although take up an area few, wide adaptability, but investment is large, energy consumption is high, and running expense is high, and seldom carries out recycling.
At present for biogas CO 2the method of removing mainly contains high-pressure washing, chemical absorption, pressure-variable adsorption and membrane separation technique etc., and cost is all higher.
In addition, micro-algae energy technology is a kind of newer biomass energy technology, because micro algae growth speed is fast, results short, photosynthetic utilising efficiency in period is high, unit surface production capacity rate high, generally believe at present it is very competitive biomass energy technology both at home and abroad, development potentiality is huge.For example, China Patent No. " 201310347109.8 " discloses a kind of natural pond liquid ecological purification method based on both culturing microalgae, and open day is 2013-11-20, comprises the steps: the liquid pre-treatment of (1) natural pond; (2) domestication of the micro-algae of autotrophic type is cultivated, acquisition can be in the liquid of 70%-100% natural pond the algae strain of Fast Growth; (3) preparation of seed liquor; (4) micro algae growth coupling natural pond liquid purifying method: the algae kind after domestication is inoculated in Race-way photobioreactor and cultivates after spreading cultivation, the method means that adopt semicontinuous cultural method and feed supplement feeding culture etc. to optimize, the chlorella biomass that obtains high-density growth purifies natural pond liquid simultaneously; (5) the biochemical broken wall of microalgae cell: microalgae cell is introduced to biochemical broken wall pond, throw in fresh-water fishes, obtain the algae slurry of broken wall; (6) gather microalgae cell recycle natural pond liquid.
But micro-algae in cultivating the consumption of the nutrient salts such as a large amount of nitrogen phosphorus and carbon source can bring huge running cost, reduce that to cultivate cost be an important factor that promotes algae energy development.Utilize the nutritive salt such as nitrogen phosphorus abundant in the liquid of natural pond to cultivate micro-algae and be considered to feasible, but because the ammonia nitrogen of the high density containing in the liquid of natural pond is unfavorable for micro algae growth, in the liquid of natural pond, a large amount of suspended matters causes transparency to reduce, and is unfavorable for the photosynthesis of micro-algae.With clear water, natural pond liquid being diluted is current conventional method, but the method can consume a large amount of water resourcess.
Summary of the invention
The object of the invention is to overcome the problems referred to above that prior art exists, provide a kind of and utilize the micro-algae of struvite precipitation coupling to cultivate the method for carrying out natural pond liquid and marsh gas purifying.The transparency that the present invention utilizes struvite precipitation technology to reduce ammonia nitrogen concentration in natural pond liquid, promote natural pond liquid, to be more conducive to the growth of micro-algae, utilizes micro-algae to CO in biogas 2purify natural pond liquid and biogas with the nutritive salt such as nitrogen phosphorus in the liquid of natural pond, can promote biogas utilization and be worth, reduce biogas engineering running cost.
For achieving the above object, the technical solution used in the present invention is as follows:
Utilize the micro-algae of struvite precipitation coupling to cultivate a method of carrying out natural pond liquid and marsh gas purifying, it is characterized in that, comprise the steps:
NH in natural pond liquid after a, mensuration breeding wastewater anaerobically fermenting 4 +, Mg 2+and PO 4 3-concentration;
B, in the liquid of natural pond, add Mg 2+and PO 4 3-generation struvite precipitation, add-on is with NH 4 +clearance reach 70%-90% for standard;
After c, struvite precipitation, natural separation solid and liquid, be adjusted to liquid pH value in the scope that is applicable to micro algae growth and cultivate for micro-algae;
D, in the liquid regulating after pH value, inoculate after micro-algae, the biogas passing into after desulfurization is cultivated;
After e, cultivation, carry out solid-liquid separation, micro-algae frond is collected to utilization.
In described step a, be after Anaerobic Digestion for the natural pond liquid of struvite precipitation, and not pretreated fresh natural pond liquid.
In described step a, breeding wastewater natural pond liquid after marsh-gas fermentation tank anaerobic digestion enters in equalizing tank, measures NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-concentration.
In described step b, natural pond liquid enters after agitated pool, according to the ionic concn of measuring and the biological characteristics of micro-algae algae kind, in the liquid of natural pond, adds Mg 2+and PO 4 3-, regulate pH value, then stir, make the struvite precipitation producing.
In described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator, between 9.0-10.0, then stirs 10-20min with the speed of 50-150rpm, reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L.
In described step b, after reaction finishes, open agitated pool gate, reaction solution is drained into settling tank, from equalizing tank, put into next batch natural pond liquid and enter agitated pool, process next batch natural pond liquid.
In described step b, the Mg adding 2+derive from magnesium salts MgCl 2, Mg (OH) 2, MgCO 3in one, PO 4 3-mainly come from sylvite KH 2pO 4, K 2hPO 4in one.
In described step c, the solid of generation and liquid mixture in rectangular sedimentation tank after the precipitation of 1-3 hour supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
In described step c, the described liquid pH value of cultivating for micro-algae is adjusted between 6.5-7.5.
In described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate in micro-algae incubator, culture cycle is 10-20d.
In described steps d, micro-algae of inoculation is micro-algae to processing rear natural pond liquid and biogas with tolerance, as the chlorella of Chlorophyta ( chlorella), grid algae ( scenedesmus), Botryococcus braunii ( botryococcus) etc.
Employing the invention has the advantages that:
One, the present invention is according to the NH in the liquid of natural pond 4 +, PO 3-, Mg 2+concentration and the demand of micro algae growth suitably add PO 3-, Mg 2+, make it under high pH condition, produce struvite precipitation, to reduce high NH 4 +on the impact of micro-algal biomass accumulation; And the suspended substance that the throwing out that utilizes the precipitation of struvite is removed in natural pond liquid increases natural pond liquid transparence, be conducive to micro-algal biomass accumulation; Utilize in micro algae growth process the absorption of nitrogen phosphorus is cut down to the nitrogen phosphorus in the rear supernatant liquor of struvite precipitation; Utilize in micro algae growth process CO in biogas 2fixing biogas is purified.
Two, the present invention can recycle the nitrogen phosphor resource in the liquid of natural pond greatly, and fixes the CO in biogas in purifying natural pond liquid 2to reach the object of marsh gas purifying, whole process can reduce the discharge of breeding wastewater anaerobically fermenting after stain thing, improves taste and the added value of biogas utilization, has higher economic benefit and environmental benefit.
Three, in step a of the present invention, natural pond liquid for struvite precipitation is after Anaerobic Digestion, and not pretreated fresh natural pond liquid, the advantage having is that anaerobic process can be killed the microorganism that is unfavorable for micro algae growth in breeding wastewater stoste, and remove a large amount of organism, be conducive to the growth of micro-algae; The struvite precipitation process that is directly used in not pretreated fresh natural pond liquid can reduce aerial open-assembly time of natural pond liquid, to reduce, the harmful biology of micro algae growth be entered to natural pond liquid, and can save processing cost.
Four, in step b of the present invention, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, adopt the advantage of this specified proportion to be can make the efficiency of struvite precipitation to reach maximization under this ratio condition, to remove more NH 4 +, and can make to retain in reacted liquid a certain amount of phosphorus and magnesium to meet the demand of micro algae growth; PH regulator is between 9.0-10.0, and the advantage having is the formation that this pH scope is suitable for struvite precipitation, and it is insufficient that too low meeting makes to react, and is unfavorable for NH 4 +removal, too high meeting makes NH 4 +with NH 3gas form volatilization, is unfavorable for the recovery of nitrogen, pollutes the environment again, and can make cost increase; Stir 10-20min with the speed of 50-150rpm again, the advantage having is the carrying out that this stirring velocity and time is conducive to precipitin reaction, and the too low or time, too short meeting affect sedimentation effect, and too high or long meeting increases running cost; Reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L, the advantage having is at this NH 4 +under concentration conditions, micro-algae can keep good growth conditions, and further removes NH by the absorption of micro-algae self 4 +with other pollutents, excessive concentration can produce and suppress micro algae growth, is unfavorable for the further purification to natural pond liquid and biogas.
Five, in step c of the present invention, produce solid and liquid mixture in rectangular sedimentation tank after the precipitation of 1-3 hour supernatant liquor from the other end flow into water collecting basin store, the solid of precipitation is extracted out dry rear as fertilizer with pump, the advantage having is can automatically carry out solid-liquid separation by leaving standstill of for some time after struvite precipitation, adopt and do not need the rectangular sedimentation tank of power to be conducive to save the cost of solid-liquid separation process, solid can increase added value as fertilizer.
Six, in step c of the present invention, the described liquid pH value of cultivating for micro-algae is adjusted between 6.5-7.5, and the advantage having is that micro-algae has good growth conditions in this pH interval, the too high or too low growth that is unfavorable for micro-algae.
Seven, in steps d of the present invention, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, in micro-algae incubator, inoculating the biogas passing into after desulfurization after micro-algae airtight incubator cultivates, culture cycle is 10-20d, the advantage having is that the growth meeting of micro-algae in this time interval proceeds to the stage of stable development from logarithmic phase, continue to cultivate and be unfavorable for obtaining and the decontamination effect improving of water quality of micro-algal biomass, and can make running cost increase.
Eight,, in described steps d, micro-algae of inoculation is micro-algae to processing rear natural pond liquid and biogas with tolerance, as the chlorella of Chlorophyta ( chlorella), grid algae ( scenedesmus), Botryococcus braunii ( botryococcus) etc., the advantage having is still to contain a certain amount of ammonia nitrogen in the natural pond liquid after struvite precipitation process, and can make salinity increase, in biogas after desulfurization, still contain part sulphur, can affect the growth of most micro-algae algae kinds, select the algae kind of tolerance to be conducive to obtain best micro algae growth state, better purify natural pond liquid and biogas, obtain higher added value.
Nine, in step b of the present invention, natural pond liquid enters after agitated pool, according to the ionic concn of measuring and the biological characteristics of micro-algae algae kind, in the liquid of natural pond, adds Mg 2+and PO 4 3-, regulate pH value, then stir, the struvite producing is precipitated, the advantage having is to add Mg 2+and PO 4 3-make the NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-mole number reaches suitable ratio, improves deposition efficiency, to remove more NH 4 +, meet the growth demand of micro-algae.
Accompanying drawing explanation
Fig. 1 is schematic flow sheet of the present invention.
Embodiment
Embodiment 1
Utilize the micro-algae of struvite precipitation coupling to cultivate a method of carrying out natural pond liquid and marsh gas purifying, mainly comprise following step:
(1) measure NH in the natural pond liquid after breeding wastewater anaerobically fermenting 4 +, Mg 2+and PO 4 3-concentration;
(2) in agitated pool, add suitable Mg according to the ionic concn of measuring and the biological characteristics of micro-algae algae kind 2+and PO 4 3-, add-on is with NH 4 +clearance reach 70%-90% for standard, regulate pH value, stir, make the struvite precipitation that produces complete;
(3) after step (2) struvite precipitin reaction finishes, solid and liquid mixture flow into settling tank, make solid and liquid natural separation, and solid is for slow release fertilizer; Liquid flows into water collecting basin from settling tank and stores, and pH value is injected in micro-algae incubator and cultivates for micro-algae after being adjusted in the scope that is applicable to micro algae growth;
(4) in micro-algae incubator, inoculating the biogas passing into after desulfurization after micro-algae airtight incubator cultivates.Give vent to anger after dehydration for combustion power generation, life energy etc.;
(5) by carrying out solid-liquid separation after cultivation for some time, micro-algae frond is collected, frond is for the extraction of biofuel, and liquid is back to plant or discharge.
The natural pond liquid precipitating for struvite is for to flow out from anaerobic digestion device, not pretreated fresh natural pond liquid, directly producing by reaction the suspended substance that throwing out that magnesium ammonium phosphate (struvite) precipitates and standing separation process remove in the liquid of natural pond enhances the transparency, and reduce the ammonia-nitrogen content in the liquid of natural pond, be beneficial to the growth of micro-algae.
The Mg adding described in step (2) 2+derive from magnesium salts MgCl 2, Mg (OH) 2, MgCO 3in one, PO 4 3-mainly come from sylvite KH 2pO 4, K 2hPO 4in one, make NH 4 +, Mg 2+and PO 4 3-ratio reach 1:1.2:1, pH regulator is between 9.0-10.0, reaction finishes NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L.Micro algae growth needs nitrogen, phosphorus, these three kinds of elements of magnesium, and in the liquid stoste of natural pond, ammonia-nitrogen content exceeds micro-algae tolerance, needs struvite precipitin reaction to remove, but NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-the precipitin reaction that ratio produces can not make NH 4 +be cut in suitable scope, so need to add Mg 2+and PO 4 3-, Mg 2+and PO 4 3-add-on is with NH 4 +clearance reach 70%-90% for standard, now the N in liquid, P, Mg can satisfy the demand.
The stirring reaction time in step (2) is 10-20min, and stirring velocity is 50-150rpm.
The liquid pH value of cultivating for micro-algae described in step (3) is adjusted between 6.5-7.5.
Described in step (4), be the micro-algae that has certain tolerance to processing rear natural pond liquid and biogas for purifying micro-algae of natural pond liquid and biogas, as the chlorella of Chlorophyta ( chlorella), grid algae ( scenedesmus), Botryococcus braunii ( botryococcus) etc.
Micro-algae incubator in step (3) and (4) is airtight ascending manner photoreactor.
Embodiment 2
As shown in Fig. 1, according to technical scheme provided by the invention, utilize be coupled micro-algae of struvite crystallization to cultivate the method for carrying out natural pond liquid and marsh gas purifying, mainly comprise following processing step:
Step 101: breeding wastewater natural pond liquid after marsh-gas fermentation tank anaerobic digestion enters in equalizing tank, and measures NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-concentration.
In this step, the effect of equalizing tank is mainly to regulate natural pond liquid to enter the amount of agitated pool, and natural pond liquid residence time in equalizing tank is shorter.
Step 102: add a certain amount of Mg according to the ionic concn of measuring and the biological characteristics of selected micro-algae algae kind 2+and PO 4 3-make struvite precipitation, add-on is with NH 4 +clearance reach 70%-90% for standard, N, P, Mg content after reaction in liquid are applicable to micro algae growth, and regulate pH value between 9.0-10.0, speed with 50-150rpm stirs 10-20min, after finishing, reaction opens agitated pool gate, reaction solution is drained into settling tank, from equalizing tank, puts into next batch natural pond liquid and enter agitated pool, process next batch natural pond liquid.
The reaction occurring in step 102 is as follows:
Mg 2+?+?NH 4 +?+?PO 4 3-?+?6H 2O?→?MgNH 4PO 4·6H 2O↓
The Mg adding in this step 2+from MgCl 2, Mg (OH) 2, MgCO 3in one, PO 4 3-come from sylvite KH 2pO 4, K 2hPO 4in one, utilize NaOH to regulate pH value.
Step 103: the solid that step 102 produces and liquid mixture in rectangular sedimentation tank after the precipitation of 1-3 hour supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
Step 104: be injected in ascending manner photoreactor after the natural pond after treatment liquid in water collecting basin is regulated to pH value, inoculate micro-algae algae kind, pass into through the biogas of desulfurization after airtight and carry out micro-algae cultivation.
In this step micro-algae algae kind used be Chlorophyta chlorella ( chlorella), grid algae ( scenedesmus), Botryococcus braunii ( botryococcus) in one.PH is transferred between 6.5-7.5.
Step 105: the biogas through micro algae culturing device processing is used for life, producting energy, generating etc. through dehydration afterfire.
Step 106: cultivate the micro-algae that reaches harvesting time in photoreactor after solid-liquid separation, frond is for the extraction of biofuel, micro-algae albumen.
Micro-algae culture cycle in this step is 10-20d.
Embodiment 3
The present embodiment natural pond used liquid is Wastewater from Pig Farm is greater than 1 month anaerobically fermenting natural pond liquid through the residence time, utilizes National Standard Method to measure the NH in the liquid of natural pond 4 +concentration is 522mg/L, Mg 2+concentration is 29mg/L, PO 4 3-concentration is 101mg/L, and natural pond liquid transparency is 6cm.In the agitated pool of 100L, add 37.5g KH 2pO 4, MgCl 26H 2o 68.2g, stirring and dissolving, with NaOH adjusting pH to 9.5, stirs with the stirring velocity of 100rpm the valve of opening bottom being arranged in after 10min and makes reaction solution flow into the standing 2h of settling tank, supernatant liquor is put into water collecting basin, more than the transparency of natural pond liquid reaches 30cm.With after HCl adjusting pH to 7.0, the natural pond liquid in water collecting basin is transferred in ascending manner photoreactor, inoculate a chlorella of this laboratory screening, the biogas after airtight rear access desulfurization, biogas air inlet CO 2concentration 36-40%, CH 4concentration 58-63%.CO after discolour silica gel dehydration gives vent to anger 2concentration is 5-12%, CH 4concentration is 80-85%.Continue to cultivate after 10 days, the chlorella of inoculation reaches harvesting time, and liquid and frond are taken out, and adopts centrifugal mode to collect the extraction of frond for grease, and in remaining liq, ammonia nitrogen is less than 50mg/L, TP and is less than 2mg/L and reaches livestock breeding wastewater emission standard.
Embodiment 4
Utilize the micro-algae of struvite precipitation coupling to cultivate a method of carrying out natural pond liquid and marsh gas purifying, comprise the steps:
NH in natural pond liquid after a, mensuration breeding wastewater anaerobically fermenting 4 +, Mg 2+and PO 4 3-concentration;
B, in the liquid of natural pond, add Mg 2+and PO 4 3-generation struvite precipitation, add-on is with NH 4 +clearance reach 70%-90% for standard;
After c, struvite precipitation, natural separation solid and liquid, be adjusted to liquid pH value in the scope that is applicable to micro algae growth and cultivate for micro-algae;
D, in the liquid regulating after pH value, inoculate after micro-algae, the biogas passing into after desulfurization is cultivated;
After e, cultivation, carry out solid-liquid separation, micro-algae frond is collected to utilization.
In described step a, be after Anaerobic Digestion for the natural pond liquid of struvite precipitation, and not pretreated fresh natural pond liquid.
In described step a, breeding wastewater natural pond liquid after marsh-gas fermentation tank anaerobic digestion enters in equalizing tank, measures NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-concentration.
In described step b, natural pond liquid enters after agitated pool, according to the ionic concn of measuring and the biological characteristics of micro-algae algae kind, in the liquid of natural pond, adds Mg 2+and PO 4 3-, regulate pH value, then stir, make the struvite precipitation producing.
In described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator, between 9.0-10.0, then stirs 10-20min with the speed of 50-150rpm, reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L.
In described step b, after reaction finishes, open agitated pool gate, reaction solution is drained into settling tank, from equalizing tank, put into next batch natural pond liquid and enter agitated pool, process next batch natural pond liquid.
In described step b, the Mg adding 2+derive from magnesium salts MgCl 2, Mg (OH) 2, MgCO 3in one, PO 4 3-mainly come from sylvite KH 2pO 4, K 2hPO 4in one.
In described step c, the solid of generation and liquid mixture in rectangular sedimentation tank after the precipitation of 1-3 hour supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
In described step c, the described liquid pH value of cultivating for micro-algae is adjusted between 6.5-7.5.
In described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate in micro-algae incubator, culture cycle is 10-20d.
In described steps d, micro-algae of inoculation is micro-algae to processing rear natural pond liquid and biogas with tolerance, as the chlorella of Chlorophyta ( chlorella), grid algae ( scenedesmus), Botryococcus braunii ( botryococcus) etc.
Embodiment 5
The present embodiment and above-described embodiment are basic identical, and the key distinction is:
In described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator to 9.0, then stir 10min with the speed of 50rpm, reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L.
In described step c, the solid of generation and liquid mixture in rectangular sedimentation tank after the precipitation of 1 hour supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
In described step c, the described liquid pH value of cultivating for micro-algae is adjusted in 6.5.
In described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate in micro-algae incubator, culture cycle is 10d.
Embodiment 6
The present embodiment and above-described embodiment are basic identical, and the key distinction is:
In described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator to 10.0, then stir 20min with the speed of 150rpm, reaction finishes the NH in rear supernatant liquor 4 +concentration is controlled at 90mg/L.
In described step c, the solid of generation and liquid mixture in rectangular sedimentation tank after the precipitation of 3 hours supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
In described step c, the described liquid pH value of cultivating for micro-algae is adjusted to 7.5.
In described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate in micro-algae incubator, culture cycle is 20d.
Embodiment 7
The present embodiment and above-described embodiment are basic identical, and the key distinction is:
In described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator to 9.5, then stir 15min with the speed of 100rpm, reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 60mg/L.
In described step c, the solid of generation and liquid mixture in rectangular sedimentation tank after the precipitation of 2 hours supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
In described step c, the described liquid pH value of cultivating for micro-algae is adjusted to 7.0.
In described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate in micro-algae incubator, culture cycle is 15d.
Above specific embodiments of the invention are described, it will be appreciated that, the present invention is not limited to above-mentioned specific implementations, and those skilled in the art can make various distortion or modification within the scope of the claims, and this does not affect flesh and blood of the present invention.

Claims (10)

1. utilize the micro-algae of struvite precipitation coupling to cultivate a method of carrying out natural pond liquid and marsh gas purifying, it is characterized in that, comprise the steps:
NH in natural pond liquid after a, mensuration breeding wastewater anaerobically fermenting 4 +, Mg 2+and PO 4 3-concentration;
B, in the liquid of natural pond, add Mg 2+and PO 4 3-generation struvite precipitation, add-on is with NH 4 +clearance reach 70%-90% for standard;
After c, struvite precipitation, natural separation solid and liquid, be adjusted to liquid pH value in the scope that is applicable to micro algae growth and cultivate for micro-algae;
D, in the liquid regulating after pH value, inoculate after micro-algae, the biogas passing into after desulfurization is cultivated;
After e, cultivation, carry out solid-liquid separation, micro-algae frond is collected to utilization.
2. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 1, it is characterized in that: in described step a, natural pond liquid for struvite precipitation is after Anaerobic Digestion, and not pretreated fresh natural pond liquid.
3. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 1 and 2, is characterized in that: in described step a, breeding wastewater natural pond liquid after marsh-gas fermentation tank anaerobic digestion enters in equalizing tank, measures NH in the liquid of natural pond 4 +, Mg 2+and PO 4 3-concentration.
4. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 3, it is characterized in that: in described step b, natural pond liquid enters after agitated pool, according to the ionic concn of measuring and the biological characteristics of micro-algae algae kind, in the liquid of natural pond, adds Mg 2+and PO 4 3-, regulate pH value, then stir, make the struvite precipitation producing.
5. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 4, is characterized in that: in described step b, in the liquid of natural pond, add Mg 2+and PO 4 3-, make NH 4 +, Mg 2+and PO 4 3-molar ratio reach 1:1.2:1, pH regulator, between 9.0-10.0, then stirs 10-20min with the speed of 50-150rpm, reaction finishes the NH in rear supernatant liquor 4 +within concentration is controlled at 100mg/L.
6. cultivate the method for carrying out natural pond liquid and marsh gas purifying according to the micro-algae of struvite precipitation coupling that utilizes described in claim 4 or 5, it is characterized in that: in described step b, after finishing, reaction opens agitated pool gate, reaction solution is drained into settling tank, from equalizing tank, put into next batch natural pond liquid and enter agitated pool, process next batch natural pond liquid.
7. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 6, is characterized in that: in described step b, and the Mg adding 2+derive from magnesium salts MgCl 2, Mg (OH) 2, MgCO 3in one, PO 4 3-mainly come from sylvite KH 2pO 4, K 2hPO 4in one.
8. cultivate the method for carrying out natural pond liquid and marsh gas purifying according to the micro-algae of struvite precipitation coupling that utilizes described in claim 1,2,4,5 or 7, it is characterized in that: in described step c, the solid producing and liquid mixture in rectangular sedimentation tank after the precipitation of 1-3 hour supernatant liquor flow into water collecting basin from the other end and store, for the solid of precipitation pump extract out dry after as fertilizer.
9. the method for utilizing the micro-algae cultivation of struvite precipitation coupling to carry out natural pond liquid and marsh gas purifying according to claim 8, is characterized in that: in described step c, the described liquid pH value of cultivating for micro-algae is adjusted between 6.5-7.5.
10. cultivate the method for carrying out natural pond liquid and marsh gas purifying according to the micro-algae of struvite precipitation coupling that utilizes described in claim 1,2,4,5,7 or 9, it is characterized in that: in described steps d, regulate the liquid after pH value to be injected into micro-algae incubator from water collecting basin, in micro-algae incubator, inoculate the biogas passing into after desulfurization after micro-algae airtight incubator and cultivate, culture cycle is 10-20d.
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