CN102642929A - Artificial light source adjusting method utilizing chlorella to purify pig manure waste water - Google Patents

Artificial light source adjusting method utilizing chlorella to purify pig manure waste water Download PDF

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CN102642929A
CN102642929A CN2012101308862A CN201210130886A CN102642929A CN 102642929 A CN102642929 A CN 102642929A CN 2012101308862 A CN2012101308862 A CN 2012101308862A CN 201210130886 A CN201210130886 A CN 201210130886A CN 102642929 A CN102642929 A CN 102642929A
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chlorella
waste water
pig manure
illumination
intensity
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郑正
颜诚
赵永军
王东
钱俊成
李博
黄威
聂耳
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Fudan University
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Fudan University
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Abstract

The invention belongs to the technical field of environmental engineering and particularly relates to an artificial light source adjusting method utilizing chlorella to purify pig manure waste water. The artificial light source adjusting method comprises: utilizing the pig manure waste water to serve as nutritional matrix for cultivating chlorella, enabling a light-emitting diode (LED) lamp with a specific wavelength to serve as an artificial light source, performing adjustment with step-changing illumination intensity, and promoting growth of chlorella economically and efficiently so as to improve pig manure waste water purifying effect. Compared with ordinary methods utilizing fixed illumination intensity, the artificial light source adjusting method can save luminous energy massively and improves economic benefits of utilizing the chlorella to purify the pig manure waste water. The artificial light source adjusting method is good in practicability, simple and practicable.

Description

A kind of source of artificial light inflation method of utilizing chlorella to purify pig manure waste water
Technical field
The invention belongs to field of environment engineering technology, be specifically related to a kind of source of artificial light inflation method of utilizing chlorella to purify pig manure waste water.
Background technology
Along with the fast development of China's economic society, China's aquaculture is also developed rapidly.Suburbs, Shanghai in 1998 just total big-and-middle-sized livestock and poultry farm more than 1000 families; Large scale of pig farm field, Hefei ,Anhui city breeding stock reaches 150,000; Pig farm more than thousand in 1994, Chengdu, Sichuan reaches family more than 120, and having more than 20 more than ten thousand is tame, also has raising scale in the super-huge large scale of pig farm field more than 100,000.The large-scale cultivation industry has also caused serious pollution threat to environment providing abundant meat, egg, milk and goods thereof to satisfy people's living needs to the town dweller when.The industrial solid castoff of China in 2002 is merely 9.45 hundred million tons, and the feces of livestock and poultry generation has just reached 27.5 hundred million tons then, is 2.91 times of industrial solid castoff total amount.Wherein pig manure is 12.9 hundred million tons, accounts for 46.91% of feces of livestock and poultry total amount.
Mainly contain anaerobic treatment process, aerobic treatment process and aerobic-anaerobic to the waste water treatment process of culturing the place employing at present and combine these 3 kinds of treatment process.Because the complicacy of plant's wastewater property is used the rarely found of anaerobic technique merely.At present the reactor drum ubiquity of plant's waste water treatment applications that processing efficiency is low, the organism volumetric loading is little, waterpower long shortcoming residence time.Processing efficiency is low, will cause later stage aerobic treatment unit working cost to increase; The mud that aerobic treatment produces also needs further to handle and dispose; Waterpower residence time increases, and will strengthen the useful volume of reactor drum, increase equipment with, these have all influenced the economic and practical of existing treatment process greatly.
The characteristics of chlorella are to have high photosynthetic efficiency, and nutritive substance such as absorbed nitrogen and phosphorus from surrounding environment that can be a large amount of rapidly can play the effect of water quality around purifying in the process of self continuous growth and breeding.The growth of chlorella meets the Changing Pattern of standby period, logarithmic phase, stationary phase and decline phase.Its early stage in growth is less because of density, and the nutrient solution transparence is better, so can just reach growth result preferably according under the intensity than low light; Later stage in growth is bigger because of the density of algae quantity, exists the influence of covering each other between frond, so need higher intensity of illumination.At the early stage of chlorella growth and the illumination method of rational adjustment of later stage source of artificial light, not only can save a large amount of luminous energy, improve the economic benefit of this treatment process; More can avoid the growth of chlorella light inhibition phenomenon to occur, and then influence the decontamination effect improving of pig manure waste water.
Summary of the invention
The object of the present invention is to provide the high chlorella that utilizes of a kind of good purification, economic benefit to purify the source of artificial light inflation method of pig manure waste water.
The source of artificial light inflation method of utilizing chlorella to purify pig manure waste water provided by the invention, the intensity of illumination inflation method of employing stepped change can effectively be improved treatment effect and economy that chlorella purifies pig manure waste water.Concrete steps are following:
(1) fixedly culture temperature, chlorella initial inoculation amount, original ph, pig manure waste strength and Light To Dark Ratio; And intensity of illumination; The growing state of chlorella and the pollutants removal rate of pig manure waste water under the LED lamp source condition of test different wave length, and select optimum LED light look wavelength;
(2) fixedly culture temperature, chlorella initial inoculation amount, original ph, pig manure waste strength and Light To Dark Ratio; And optimum source of artificial light light-color wavelengths; The growing state of chlorella and the pollutants removal rate of pig manure waste water under the test different illumination intensity condition; Select optimum intensity of illumination scope, and adopt the intensity of illumination inflation method of stepped change;
(3) calculate under each intensity of illumination of stepped change the economic benefit that all contaminations is removed in the cow dung waste water respectively;
(4) through contrast, confirm final stepped change inflation method about light-color wavelengths and intensity of illumination.
Among the present invention, the stepped change of said intensity of illumination is the adaptive phase according to chlorella growth in time, and logarithmic phase, stationary phase and decline phase are divided.
Among the present invention, the upper limit of the intensity of illumination scope of stepped change is the intensity of illumination when producing the light inhibition, and lower limit is the intensity of illumination when producing the illumination deficiency.
The invention has the advantages that:
(1) adopts the LED lamp as source of artificial light,,, in energy efficient, can also effectively promote the growth of chlorella so can produce some specific light quality wavelength to the chlorella chloroplast(id) because of it has the spectrum band narrower than common fluorescent lamp;
The illumination method of the stepped change that (2) adopts is to go up from the time to divide according to the growth cycle of chlorella, has distinguished its metabolic characteristic at adaptive phase, logarithmic phase, stationary phase and decline phase;
The chlorella of the little algae of inoculation of (3) being adopted; The advantages such as high photosynthetic efficiency that it not only has general algae and is had; And it can also produce some specific meta-bolites with certain economic value through regulating the nutritional medium composition in metabolic process, the possibility as biodiesel is also arranged because fat content is higher himself.
Description of drawings
Fig. 1 different wave length LED light shines condition: (a) chlorella dry weight; (b) COD clearance; (c) TN clearance; (d) TP clearance.
Fig. 2 different illumination intensity condition: (a) chlorella dry weight; (b) COD clearance; (c) TN clearance; (d) TP clearance.
The pollutants removal rate economic benefit of Fig. 3 source of artificial light inflation method.
Embodiment
Through embodiment the present invention is carried out detailed explanation below.
A kind of source of artificial light inflation method of utilizing chlorella to purify pig manure waste water; The nutraceutical matrix that utilizes pig manure waste water to cultivate as chlorella; The LED lamp of some specific wavelength comes as source of artificial light; With the intensity of illumination inflation method of stepped change, the promotion chlorella growth of realizing economical and efficient is to promote the order of pig manure purification of waste water effect.
Below be concrete step:
(1) adopt chlorella ( Chlorella Vulgaris); With fixing 25 degrees centigrade of culture temperature, initial pH=6.5, chlorella rate of vaccination is 1:3, and Light To Dark Ratio is 12h:12h, under 2000Lux intensity of illumination condition; Test its dry weight and pollutant chemistry oxygen requirement (COD under white, red, blue, green, Huang and purple wavelength LED lamp source; Chemical oxygen demand), the degradation effect of total nitrogen (TN, total nitrogen) and total phosphorus (TP, total phosphorus).
The result show for chlorella under red wavelength LED light irradiation condition dry weight each stage of growth all reach the highest (Fig. 1 a), and its contaminant degradation rate also is the highest (Fig. 1 b-1d).So can select LED red wavelength light source as source of artificial light.
(2) adopt chlorella ( Chlorella Vulgaris); So that fixedly 25 degrees centigrade of culture temperature, initial pH=6.5, chlorella rate of vaccination are 1:3; Light To Dark Ratio is 12h:12h; Under 400Lux, 800Lux, 1200Lux, 1600Lux and 2000Lux intensity of illumination condition, test the degradation effect of its dry weight and COD, TN and TP under red wavelength LED lamp source.
By the variation of chlorella dry weight among Fig. 2 a, the growth that can draw chlorella can be divided into adaptive phase, logarithmic phase, stationary phase and the decline phase that corresponds respectively to 0-48h-96h-144h-240h four-stage.The degraded of analyzing pollutent is situation (Fig. 2 b-bd) over time, the source of artificial light inflation method should for: 0-48h-96h-144h three phases, each stage is adopted intensity of illumination 800Lux, 1200Lux and 1600Lux successively.
Table 1 LED lamp character
Figure 415580DEST_PATH_IMAGE001
(3), calculate 0-48h-three stages of 96h-144h and adopt the economic benefit of the source of artificial light inflation method of intensity of illumination 800Lux, 1200Lux and 1600Lux according to the data of table 1.Its method of calculation are divided by its spent electric energy with pollutants removal rate.The result is as shown in Figure 3, shows to adopt the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water of the present invention can obtain the very strong pollutant removal of economic and practical.

Claims (6)

1. source of artificial light inflation method of utilizing chlorella to purify pig manure waste water is characterized in that concrete steps are:
(1) fixedly culture temperature, chlorella initial inoculation amount, original ph, pig manure waste strength and Light To Dark Ratio; And intensity of illumination; The growing state of chlorella and the pollutants removal rate of pig manure waste water under the LED lamp source condition of test different wave length, and select optimum LED light look wavelength;
(2) fixedly culture temperature, chlorella initial inoculation amount, original ph, pig manure waste strength and Light To Dark Ratio; And optimum source of artificial light light-color wavelengths; The growing state of chlorella and the pollutants removal rate of pig manure waste water under the test different illumination intensity condition; Select optimum intensity of illumination scope, and adopt the intensity of illumination inflation method of stepped change;
(3) calculate under each intensity of illumination of stepped change the economic benefit that all contaminations is removed in the cow dung waste water respectively;
(4) through contrast, confirm final stepped change inflation method about light-color wavelengths and intensity of illumination.
2. the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water according to claim 1 is characterized in that said pollutants removal rate is meant the degradation effect of COD, total nitrogen and total phosphorus.
3. the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water according to claim 2 is characterized in that the stepped change of said intensity of illumination is confirmed according to adaptive phase, logarithmic phase, stationary phase and the decline phase of chlorella growth in time.
4. the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water according to claim 3 is characterized in that, the upper limit of the intensity of illumination scope of stepped change is the intensity of illumination when producing the light inhibition, and lower limit is the intensity of illumination when producing the illumination deficiency.
5. the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water according to claim 4 is characterized in that, the LED lamp adopts red wavelength LED lamp.
6. the source of artificial light inflation method of utilizing chlorella to purify pig manure waste water according to claim 5 is characterized in that the adaptive phase of chlorella growth, logarithmic phase, stationary phase and decline phase time corresponding section are: 0-48h; 48h-96h, 96h-144h, 144h-240h; Corresponding therewith, the mode of intensity of illumination gradient adjustment is: 0-48h, 48h-96h; 96h-144h three phases adopts intensity of illumination to be respectively 800Lux, 1200Lux and 1600Lux.
CN2012101308862A 2012-05-01 2012-05-01 Artificial light source adjusting method utilizing chlorella to purify pig manure waste water Pending CN102642929A (en)

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CN105733950A (en) * 2014-12-10 2016-07-06 中国农业大学 A method of collecting chlorella cultured in pig farm waste water
CN105733949A (en) * 2014-12-10 2016-07-06 中国农业大学 A method of rapidly flocculating chlorella in pig farm waste water
CN105985917A (en) * 2015-02-06 2016-10-05 中国农业大学 Method for improving biomass of chlorella in swine wastewater
CN113293103A (en) * 2021-06-04 2021-08-24 江南大学 Method for improving biomass of chlorella based on corn starch processing wastewater
RU2803304C1 (en) * 2022-11-21 2023-09-12 Вита Юрьевна Белоусова Method for wastewater biological treatment

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Publication number Priority date Publication date Assignee Title
CN105733950A (en) * 2014-12-10 2016-07-06 中国农业大学 A method of collecting chlorella cultured in pig farm waste water
CN105733949A (en) * 2014-12-10 2016-07-06 中国农业大学 A method of rapidly flocculating chlorella in pig farm waste water
CN105985917A (en) * 2015-02-06 2016-10-05 中国农业大学 Method for improving biomass of chlorella in swine wastewater
CN105985917B (en) * 2015-02-06 2019-12-13 中国农业大学 Method for increasing biomass of chlorella in pig-raising wastewater
CN113293103A (en) * 2021-06-04 2021-08-24 江南大学 Method for improving biomass of chlorella based on corn starch processing wastewater
RU2803304C1 (en) * 2022-11-21 2023-09-12 Вита Юрьевна Белоусова Method for wastewater biological treatment

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Application publication date: 20120822