CN115557627A - Holothurian culture water quality restoration agent and preparation method thereof - Google Patents
Holothurian culture water quality restoration agent and preparation method thereof Download PDFInfo
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- CN115557627A CN115557627A CN202211054839.4A CN202211054839A CN115557627A CN 115557627 A CN115557627 A CN 115557627A CN 202211054839 A CN202211054839 A CN 202211054839A CN 115557627 A CN115557627 A CN 115557627A
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- water quality
- restoration agent
- culture water
- holothurian culture
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 75
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 36
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 230000000813 microbial effect Effects 0.000 claims abstract description 3
- 238000002156 mixing Methods 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 15
- 239000002068 microbial inoculum Substances 0.000 claims description 14
- 241000193755 Bacillus cereus Species 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 239000012286 potassium permanganate Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 241000006381 Bacillus flexus Species 0.000 claims description 7
- 241000255789 Bombyx mori Species 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 241000186361 Actinobacteria <class> Species 0.000 claims description 2
- 241000589516 Pseudomonas Species 0.000 claims description 2
- 241000382353 Pupa Species 0.000 claims description 2
- 241000187395 Streptomyces microflavus Species 0.000 claims description 2
- 241000235342 Saccharomycetes Species 0.000 claims 1
- 238000009360 aquaculture Methods 0.000 abstract description 6
- 244000144974 aquaculture Species 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 241000193830 Bacillus <bacterium> Species 0.000 description 6
- 241000965254 Apostichopus japonicus Species 0.000 description 5
- 235000012054 meals Nutrition 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 206010011409 Cross infection Diseases 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 208000009132 Catalepsy Diseases 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 206010053262 Skin swelling Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010047853 Waxy flexibility Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/722—Oxidation by peroxides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a holothurian culture water quality repairing agent and a preparation method thereof in the technical field of aquaculture, the holothurian culture water quality repairing agent is composed of a microbial agent, a nutrient solution and a water treatment solution according to a mass ratio of 2-5.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a holothurian culture water restoration agent and a preparation method thereof.
Background
From the development process of the aquaculture industry in recent years, almost all aquaculture varieties undergo the evolution process from prosperity to decline, and the reasons are that besides market factors, the influence of diseases sometimes plays a leading role. Except that the period is maintained for a long time. The main disease types of the existing stichopus japonicus culture method are as follows: parasitic diseases, skin ulceration, swelling and distending disease of the peristomal area, intestinal overflow, body distortion, and catalepsy. It may be caused by infection and invasion of Stichopus japonicus (host) by microbial pathogens (bacteria, virus, mycoplasma, chlamydia, rickettsia, harmful protozoa, parasite, etc.). The causes of diseases and death of the stichopus japonicus can also be said to be manifold. The following factors are mainly included: the offshore water area is seriously polluted, and the water quality condition along the shore is changed due to the damage of the ecological environment. Data statistics shows that more than 85% of breeding diseases are caused by poor water quality and environmental conditions. The water quality problem is one of the important causes for the disease attack of the stichopus japonicus. Under the influence of rainfall factors, the fresh water ratio in the pool is increased, and the salinity is changed sharply, so that the death of the stichopus japonicus is also caused. The introduction of foreign diseases causes cross infection, and leads to serious consequences of spreading and expanding. If the foreign sea cucumber fries are purchased blindly for overwintering or cultivation, the sick fries can be introduced, thereby generating cross infection.
In recent years, most of breeding units adopt the shrimp pond seeds to carry out breeding, and the problems of low disease resistance of larvae and the like caused by serious degeneration and variation of the shrimp pond seeds and multi-generation breeding are also a reason for generating diseases and high mortality. In addition, the death rate difference between the big seedlings and the small seedlings put in the culture pond is large, the disease resistance of the big seedlings and the ecological cultured seedlings is strong, the death rate is low, and the disease resistance of the small seedlings put in the culture pond is poor, and the death rate is high. Poor management, weak measures and lack of scientific management consciousness. If the culture pond is aged without reconstruction or is not reconstructed in place, the seedling laying density exceeds the bearing capacity, and the blind laying of diseased seedlings and the like are all important reasons for generating diseases.
At present, the microbial preparation in the aquaculture water is mostly used for putting photosynthetic bacteria, but cannot become the main force for improving the micro-ecology and the water quality due to the limitation of the metabolic mechanism of the photosynthetic bacteria, and other microbes which have strong decomposition conversion and adaptability and are harmless to aquaculture organisms and human beings are selected. Most bacteria of the genus bacillus meet this requirement and are themselves common species in fish ponds. The bacillus is gram-positive, is a ubiquitous aerobic bacterium, belongs to the genus bacillus, can secrete various enzymes with strong activity, can exist in a sporophyte form in the life process, is easy to produce and store, and has wide prospects as a feed microecological additive and a water microecological regulator. However, a single bacillus has a poor effect, cannot repair a water body, cannot effectively guarantee the water body quality, and influences the growth of the sea cucumbers.
Disclosure of Invention
The invention aims to provide a holothurian culture water quality restoration agent and a preparation method thereof, and aims to solve the problems that a single bacillus provided in the background technology has poor effect, can not restore a water body, can not effectively guarantee the water body quality, and influences the growth of holothurians.
In order to achieve the purpose, the invention provides the following technical scheme: the holothurian culture water quality restoration agent is composed of a microbial inoculum, a nutrient solution and a water treatment solution in a mass ratio of (2-5).
Preferably, the microbial inoculum consists of bacillus subtilis, bacillus cereus, bacillus curvatus and special bacteria for the septic tank according to a mass ratio of 2-3.
Preferably, the special bacteria for the septic tank consist of pseudomonas, actinomycetes, yeast and streptomyces microflavus according to a mass ratio of 1-4.
Preferably, the nutrient solution consists of silkworm chrysalis meal and sodium chloride according to a mass ratio of 1.
Preferably, the water treatment liquid consists of potassium permanganate and peroxyamide according to a mass ratio of 1-2.
A preparation method of the holothurian culture water restoration agent according to any one of claims 1 to 5, comprising the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank according to a proportion under the conditions that the pH value is 7.5-7.9 and the temperature is 30-33 ℃ to prepare a microbial inoculum;
s2: adding water into pupa Bombycis powder and sodium chloride at a certain proportion, adding into a mixer, and mixing at 40-45 deg.C under normal pressure to obtain nutrient solution;
s3: adding water into potassium permanganate and peroxyamide according to a proportion, putting into a stirrer, and mixing and stirring at the temperature of 70-80 ℃ and under the pressure of 1-2MPa to prepare water treatment liquid;
s4: the prepared microbial inoculum, nutrient solution and water treatment solution are mixed according to a proportion at the temperature of 35-38 ℃ under the conditions of pH of 7-7.5 and pressure of 0.8-1.2MPa to generate the water quality repairing agent.
Compared with the prior art, the invention has the beneficial effects that: according to the holothurian culture water restoration agent and the preparation method thereof, holothurian culture water is restored through the microbial inoculum and the water treatment solution, so that the quality of the holothurian culture water is effectively improved, and the normal growth of holothurian is guaranteed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a holothurian culture water quality repairing agent and a preparation method thereof, which repairs holothurian culture water through a microbial inoculum and a water treatment solution, effectively improves the water quality of the holothurian culture water, ensures the normal growth of holothurian, the holothurian culture water quality repairing agent consists of the microbial inoculum, a nutrient solution and the water treatment solution according to a mass ratio of 2-5.
The invention also provides a preparation method of the holothurian culture water quality restoration agent, which comprises the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank together according to the mass ratio of (2-3);
s2: adding water into the silkworm chrysalis meal and the sodium chloride according to a mass ratio of 1-6, putting into a mixer, and mixing under the conditions that the temperature is 40-45 ℃ and the pressure is normal pressure to prepare a nutrient solution;
s3: adding water into potassium permanganate and peroxyamide according to a mass ratio of 1-2 to the mixture, putting the mixture into a stirrer, and mixing and stirring the mixture at the temperature of 70-80 ℃ and under the pressure of 1-2MPa to prepare water treatment solution;
s4: mixing the prepared microbial inoculum, nutrient solution and water treatment solution according to the mass ratio of 2-5.
Example 1
The preparation method of the holothurian culture water restoration agent comprises the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank together according to the mass ratio of (2);
s2: adding water into the silkworm chrysalis meal and sodium chloride according to a mass ratio of 1;
s3: adding water into potassium permanganate and peroxyamide according to a mass ratio of 1;
s4: mixing the prepared microbial inoculum, nutrient solution and water treatment solution according to the mass ratio of 2.
Example 2
The preparation method of the holothurian culture water quality repairing agent comprises the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank together according to the mass ratio of (3);
s2: adding water into the silkworm chrysalis meal and the sodium chloride according to a mass ratio of 1;
s3: adding water into potassium permanganate and peroxyamide according to the mass ratio of 2 to 1, putting the mixture into a stirrer, and mixing and stirring the mixture under the conditions that the temperature is 75 ℃ and the pressure is 1.5MPa to prepare water treatment liquid;
s4: and mixing the prepared microbial inoculum, nutrient solution and water treatment solution according to the mass ratio of 3.
Example 3
The preparation method of the holothurian culture water quality repairing agent comprises the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank together according to the mass ratio of (3);
s2: adding water into silkworm chrysalis meal and sodium chloride according to a mass ratio of 1;
s3: adding water into potassium permanganate and peroxyamide according to a mass ratio of 2;
s4: mixing the prepared microbial inoculum, nutrient solution and water treatment solution according to the mass ratio of 5.
While the invention has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the various features of the embodiments disclosed herein may be used in any combination, provided that there is no structural conflict, and the combinations are not exhaustively described in this specification merely for the sake of brevity and conservation of resources. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
Claims (6)
1. A holothurian culture water quality repairing agent is characterized in that: the holothurian culture water restoration agent is composed of a microbial inoculum, a nutrient solution and a water treatment solution in a mass ratio of 2-5.
2. The holothurian culture water quality restoration agent and the preparation method thereof according to claim 1, wherein the holothurian culture water quality restoration agent comprises the following components in percentage by weight: the microbial agent consists of bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank according to the mass ratio of (2-3).
3. The holothurian culture water quality restoration agent and the preparation method thereof according to claim 2, wherein the holothurian culture water quality restoration agent comprises the following components in percentage by weight: the special bacteria for the septic tank consist of pseudomonas, actinomycetes, saccharomycetes and streptomyces microflavus according to the mass ratio of 1.
4. The holothurian culture water quality restoration agent and the preparation method thereof according to claim 3, wherein the holothurian culture water quality restoration agent comprises the following components in percentage by weight: the nutrient solution consists of silkworm chrysalis powder and sodium chloride according to a mass ratio of 1.
5. The holothurian culture water quality restoration agent and the preparation method thereof according to claim 4, wherein the holothurian culture water quality restoration agent comprises the following components in percentage by weight: the water treatment liquid consists of potassium permanganate and peroxyamide according to a mass ratio of 1-2.
6. A preparation method of the holothurian culture water restoration agent according to any one of claims 1 to 5, characterized by comprising the following steps: the preparation method of the holothurian culture water restoration agent comprises the following steps:
s1: mixing bacillus subtilis, bacillus cereus, bacillus flexus and special bacteria for a septic tank according to a proportion under the conditions that the pH value is 7.5-7.9 and the temperature is 30-33 ℃ to prepare a microbial inoculum;
s2: adding water into pupa Bombycis powder and sodium chloride at a certain proportion, adding into a mixer, and mixing at 40-45 deg.C under normal pressure to obtain nutrient solution;
s3: adding water into potassium permanganate and peroxyamide according to a proportion, putting into a stirrer, and mixing and stirring at the temperature of 70-80 ℃ and under the pressure of 1-2MPa to prepare water treatment liquid;
s4: the prepared microbial inoculum, nutrient solution and water treatment solution are mixed according to a proportion at the temperature of 35-38 ℃ under the conditions of pH 7-7.5 and pressure of 0.8-1.2MPa to generate the water quality restoration agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211054839.4A CN115557627A (en) | 2022-08-31 | 2022-08-31 | Holothurian culture water quality restoration agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211054839.4A CN115557627A (en) | 2022-08-31 | 2022-08-31 | Holothurian culture water quality restoration agent and preparation method thereof |
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| Publication Number | Publication Date |
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| CN115557627A true CN115557627A (en) | 2023-01-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211054839.4A Pending CN115557627A (en) | 2022-08-31 | 2022-08-31 | Holothurian culture water quality restoration agent and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
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2022
- 2022-08-31 CN CN202211054839.4A patent/CN115557627A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
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