CN102613444B - Breeding method of leech juveniles, liquid feed and preparation method thereof - Google Patents
Breeding method of leech juveniles, liquid feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a liquid feed of leech juveniles and a method for breeding leech juveniles by using the liquid feed. The feed is obtained by sufficiently and uniformly mixing 200-300 ml bovine serum, 30-60 g raw eggs, 30-50 g glucose, 10-25 g soybean meal, 1-5 g calcium carbonate, 0.01-0.05 % of complex vitamin, 5-15 g blood powder and 3-7 g yeast according to a formula, adding distilled water to a mixture to 500 ml, and adjusting the pH value of the mixture to 7.0. The breeding method comprises the following steps of: absorbing the liquid feed by using a large-aperture sponge with specification of 300x250x30 mm as a carrier; and reducing the long growth period of the leeches from juveniles to youths to 20 days by using a method of enabling hatched leech juveniles to naturally suck the feed on the sponge according to the density of 1500 juveniles per juvenile cylinder, wherein the average individual weight is about 0.28 g per one juvenile and the survival rate is kept at more than 96%. By using the feeding method of the liquid feed, provided by the invention, the optimal number and qualities of commercial juveniles can be achieved.
Description
Technical field
The present invention relates to leech and propagate field artificially, relate in particular to liquid feed formula and the method for breeding of a kind of leech seedling.
Background technology
Leech is under the jurisdiction of Annelida (Annelida) on animal classification, and the general designation of Hirudinea (Hirrdinea), be commonly called as leech, leech and interior awl etc.Approximately have in the world 500 kinds at present, China has found approximately there are 10 kinds.Leech as herein described refers to eurysome golden thread leech (Whitmania pigra Whitman).Under the nature condition, usually live in freshwater lake, river, pond, irrigation canals and ditches and rice terrace.Eurysome golden thread leech is not sucked blood, and mainly take the body fluid of sucking invertebrate or parenchima as main, as spiral shell, freshwater mussel, earthworm, aquatic insect larva and planktonic organism etc., sometimes takes food the humus in the water surface or bank soil yet.Leech is the medicinal aquatic animal of the traditional special type of China, is " one of Chinese medicine that Chinese pharmacopoeia (version in 2010) is included.Its dried product is concocted the rear traditional Chinese medical science and is used as medicine, and has the effects such as treatment apoplexy, hypertension, the clear stasis of blood, amenorrhoea, traumatic injury.It is the primary raw material of dredging at present the Chinese patent drug of numerous treatment cardiovascular and cerebrovascular diseases such as blood is logical, brain blood health.Along with modern medicine, to the deepening continuously and develop of leech, discovered in recent years leech preparation has special efficacy at anticancer aspect, and therefore, its medical value improves day by day, leech one of the well sold and in short supply Chinese medicine that become international.Because of the abuse such as agricultural chemicals, chemical fertilizer and industrial or agricultural " three wastes " pollution to environment in recent years, wild natural resources falls sharply, and along with the deep development of leech medical value, its domestic and international market potential of demand is huge.
Relevant leech rearing method is a lot, comprising the artificial breeding seedling.Can commercial seedling on market sell the leech seedling and normally select approximately leech seedling about 1 monthly age (body weight 0.19-0.28g/ bar, or about the long 1.7-2.6cm of natural situation lower body).Yet, just hatched the common body weight 0.05-0.08g/ bar of leech larva out, the long 0.4-0.8cm of body.Now can't directly take food the mollusks such as spiral shell, if the seedling that will just hatch is out put into culturing pool, its death rate, up to 85-90%, badly influences leech output.So needing artificial nursing meticulously to reach commercial seedling just can put in a suitable place to breed in field, pool and pond and be raised later.The method preparation of becoming thoroughly decomposed of the pile fermentations such as the employing weeds that at present the leech starter diet has and excrement of animals; Some employing fish meal, shrimp med and fresh spiral shell or freshwater mussel stir and form after pulverizing.For immature leech seedling, starter diet prepared by said method is subalimentation on the one hand, and growth of seedling is slow; Utilization rate is low on the other hand, and because feeding method in water body is directly rendered in many employings, remaining residue tends to the polluted-water environment that rots, and causes immature leech mortality, directly has influence on output and the quality of leech finished product.Therefore, from the height that hatches leech seedling survival rate its early stage artificial feeding process, be the key point that is related to the hirudiniculture win or lose.
Therefore, for the technical problem existed in prior art, be necessary to provide liquid feed formula and the method for breeding of a kind of leech seedling of improvement, to overcome above-mentioned defect.
Summary of the invention
In view of this, the object of the present invention is to provide liquid feed and the method for breeding of the leech seedling of a kind of output that can improve the leech finished product and quality.
For achieving the above object, the invention provides following technical scheme:
The liquid feed of a kind of leech seedling, it comprises 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal, 3-7g saccharomycete and distilled water 500ml.
Preferably, in the liquid feed of above-mentioned leech seedling, described liquid feed comprises 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04% B B-complex (w/v), 10g blood meal, 5g saccharomycete and distilled water 500ml.
The collocation method of the liquid feed of a kind of leech seedling, the method is specially: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal and 3-7g saccharomycete are fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.
The method for breeding of a kind of leech seedling is characterized in that: this method for breeding right to use requires 1 described liquid feed, and this method for breeding comprises the steps:
(1) hatching of leech seedling:
A1, selective body focus on the physically fit adult kind leech of 18-22g/ bar, put into the glass cylinder of growing seedlings and lay eggs after sterilization, control in the cylinder of growing seedlings water temperature at 20 ℃, and the leech mating also pierces in cylinder a side middle product cocoon that loosens the soil;
A2, the leech rear 9-11 days of laying eggs starts to artificially collect, and gets the ovum cocoon of giving birth in 3 days and is emitted on the loose moisturizing earth surfaces of an incubator bottom, and the incubator side, with air-vent, covers 2 layers of gauze on the ovum cocoon, covers the soft earth of one deck on gauze again;
A3, incubator is concentrated and to be put into a climatic chamber, temperature is controlled at 24-26 ℃, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH;
A4,5-10 days leech seedling of process hatch out;
(2) the leech seedling raises and train
B1, the wide-aperture sponge of 300 * 250 * 30mm specification is immersed in liquid feed to 1 hour, allows sponge fully receive liquid feed;
B2, the leech seedling that will just hatch out are put into according to constant density glass that bottom is placed with sponge and grow seedlings in cylinder, and hide gauze in order to avoid the leech seedling escapes above the cylinder of growing seedlings;
B3, every day and evening allow the leech seedling suck on sponge each 2 hours at 7 at 11 respectively in the morning, and adopt water spray means to keep growing seedlings cylinder humidity;
B4, each leech suck rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with the nylon mesh screen, the leech seedling is fished for out immediately, discards salt solution, and sponge is clean standby;
B5, the leech of pulling out move to respectively in the cylinder of growing seedlings that depth of water 15cm is housed in addition, and the top of the cylinder of growing seedlings is provided with anti-escape net, and cobble is put into as base in bottom, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour;
Repeat B6, every day the feeding process of B3, change the liquid feed in sponge every day.
Preferably, in the method for breeding of above-mentioned leech seedling, in described step B2, the density of leech seedling is 1500.
Preferably, in the method for breeding of above-mentioned leech seedling, the leech seedling that described method for breeding is raised is eurysome golden thread leech.
Preferably, in the method for breeding of above-mentioned leech seedling, the described glass cylinder specification of growing seedlings is 40 * 30 * 20cm.
Preferably, in the method for breeding of above-mentioned leech seedling, the nylon mesh screen in described step B4 is 80 order nylon mesh screens.
Preferably, in the method for breeding of above-mentioned leech seedling, in described step B5, water body pH is between 6～7, and water temperature remains between 20-30 ℃, and dissolved oxygen amount is greater than 8 mg/litre.
From technique scheme, can find out, the liquid feed of the leech seedling of the embodiment of the present invention and use this liquid feed to raise the method for leech seedling, can make seedling to young seedling shorten to 20 days growth period, average whose body weight is in 0.28g/ bar left and right, and survival rate remains on more than 96%, use the feeding method of liquid feed of the present invention can reach the best commodity quality and quantity with seedling.
The specific embodiment
The present invention is according to just hatching the leech seedling can only suck small aquatile or aquatic animal body fluid in natural environment, can not take food than the characteristics of mcroorganism body, through repetition test research, creates out.
The invention discloses the liquid feed of a kind of leech seedling, it comprises 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal, 3-7g saccharomycete and distilled water 500ml.
In preferred embodiment, liquid feed comprises 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04% B B-complex (w/v), 10g blood meal, 5g saccharomycete and distilled water 500ml.
The invention also discloses the collocation method of the liquid feed of a kind of leech seedling, the method is specially: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal and 3-7g saccharomycete are fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.
The invention also discloses the method for breeding of a kind of leech seedling, this method for breeding is used above-mentioned liquid feed, and this method for breeding comprises the steps:
(1) hatching of leech seedling:
A1, selective body focus on the physically fit adult kind leech of 18-22g/ bar, put into the glass cylinder of growing seedlings and lay eggs after sterilization, control in the cylinder of growing seedlings water temperature at 20 ℃, and the leech mating also pierces in cylinder a side middle product cocoon that loosens the soil;
A2, the leech rear 9-11 days of laying eggs starts to artificially collect, and gets the ovum cocoon of giving birth in 3 days and is emitted on the loose moisturizing earth surfaces of an incubator bottom, and the incubator side, with air-vent, covers 2 layers of gauze on the ovum cocoon, covers the soft earth of one deck on gauze again;
A3, incubator is concentrated and to be put into a climatic chamber, temperature is controlled at 24-26 ℃, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH;
A4,5-10 days leech seedling of process hatch out;
(2) the leech seedling raises and train
B1, the wide-aperture sponge of 300 * 250 * 30mm specification is immersed in liquid feed to 1 hour, allows sponge fully receive liquid feed;
B2, the leech seedling that will just hatch out are put into according to constant density glass that bottom is placed with sponge and grow seedlings in cylinder, and hide gauze in order to avoid the leech seedling escapes above the cylinder of growing seedlings;
B3, every day and evening allow the leech seedling suck on sponge each 2 hours at 7 at 11 respectively in the morning, and adopt water spray means to keep growing seedlings cylinder humidity;
B4, each leech suck rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with the nylon mesh screen, the leech seedling is fished for out immediately, discards salt solution, and sponge is clean standby;
B5, the leech of pulling out move to respectively in the cylinder of growing seedlings that depth of water 15cm is housed in addition, and the top of the cylinder of growing seedlings is provided with anti-escape net, and cobble is put into as base in bottom, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour;
Repeat B6, every day the feeding process of B3, change the liquid feed in sponge every day.
Further, in described step B2, the density of leech seedling is 1500.
Further, the leech seedling that described method for breeding is raised is eurysome golden thread leech.
Further, the described glass cylinder specification of growing seedlings is 40 * 30 * 20cm.
Further, the nylon mesh screen in described step B4 is 80 order nylon mesh screens.
Further, in described step B5, water body pH is between 6～7, and water temperature remains between 20-30 ℃, and dissolved oxygen amount is greater than 8 mg/litre.
The liquid feed of the disclosed leech seedling of the invention described above and use this liquid feed to raise the method for leech seedling, both be applicable to the required whole nutrition of leech seedling development, can shorten again the growth period of leech seedling.
Below will the technical scheme in the embodiment of the present invention be described in detail, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, the every other embodiment that those of ordinary skills obtain under the prerequisite of not making creative work, belong to the scope of protection of the invention.
The present invention leech seedling used adopts in the constant indoor temperature constant humidity cabinet concentrates hatching, and selective body focuses on the physically fit adult kind leech of 18-22g/ bar, puts into the glass cylinder of growing seedlings and lay eggs after sterilization, controls in the cylinder of growing seedlings water temperature at 20 ℃.The leech mating also pierces in cylinder a side middle product cocoon that loosens the soil.Leech lays eggs and starts to artificially collect in latter about 10 days.Get the ovum cocoon of giving birth in 3 days and be emitted on the loose moisturizing earth surfaces of incubator bottom, then on the ovum cocoon, cover 2 layers of gauze, cover the soft earth of the about 1cm of a layer thickness again on gauze, the incubator side is with air-vent.Incubator is concentrated and put into climatic chamber, and temperature is controlled at 25 ℃ of left and right, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH.Through one week left and right leech, hatch out, now the seedling body weight is between the 0.05-0.08g/ bar, and natural situation lower body length is between 0.4-0.8cm.The humiture of effectively controlling the leech hatching can make the leech cycle of emerging shorten, and the concentration degree of emerging is high, is convenient to next step and just incubates the seedling feeding and management.
The part that the present invention raises the liquid feed of leech seedling comprises 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal, 3-7g saccharomycete and distilled water 500ml.In the reality preparation, can be according to specifically need in aforementioned number range, selecting the composition of requirement.In preferred embodiment, liquid feed comprises 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04% B B-complex (w/v), 10g blood meal, 5g saccharomycete and distilled water 500ml.
The collocation method of the liquid feed of this leech seedling is: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05% B B-complex (w/v), 5-15g blood meal and 3-7g saccharomycete are fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.
The method for breeding that adopts the aforesaid liquid feed to raise the leech seedling comprises:
Leech seedling feeding process: sponge is immersed in the fluid openings feed to 1 hour, allows sponge fully receive the fluid openings feed.By the leech seedling that just hatches out, get respectively the varying number leech and be put on sponge, move in the cylinder of growing seedlings.And evening allow leech suck on sponge each 2 hours at 7 at 11 in the morning every day respectively, now adopts water spray means to keep growing seedlings cylinder humidity.Growing seedlings, the cylinder top hides gauze in order to avoid the leech escape.Each leech sucks rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with 80 order nylon mesh screens, leech is fished for immediately, discards salt solution, and sponge is clean standby.The leech of pulling out moves to respectively in the cylinder of growing seedlings that water is housed in addition.The grow seedlings top of cylinder is provided with anti-escape net, and cobble is put into as base in bottom.Control is grown seedlings the cylinder water temperature at 25 ± 2 ℃, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour.Repeat subsequently above-mentioned feeding process every day, observed and recorded leech upgrowth situation, reject dead leech in time, the statistics survival rate.Get at random 100 every 5 days and weigh, change fresh liquid feed in sponge every day.
Design by experiment, through statistical analysis and relatively discovery, in identical feeding and management situation, different leech Seedling densities and its leech speed of growth, survival rate all have certain difference.Finishing screen is selected and is reached the requirement of commercial leech seedling, makes seedling to young seedling (commercial seedling) shorten to 20 days growth period, and average whose body weight is in 0.28g/ bar left and right, and survival rate remains on more than 96%.
Below in conjunction with concrete test example, the present invention will be further described.
Test example one
1. material and instrument
Kind of leech (being bred by University Of Suzhou's Experiment of Zoology chamber) is through being accredited as eurysome golden thread leech (Whitmania pigra Whitman), cow's serum, raw egg, glucose, dregs of beans, calcium carbonate, B B-complex, blood meal and saccharomycete, 40 * 30 * 20cm specification glass grow seedlings large aperture sponge, nylon net cloth (standard 80 orders), electronic balance (U.S. OHAUS, the model: AR1530/C), oxygen charging pump, HS-250SC climatic chamber of cylinder, 300 * 250 * 30mm specification.
2. method
2.1 experimental design
2.2 the hatching of leech seedling: selective body focuses on the physically fit adult kind leech of 18-22g/ bar.Put into the glass cylinder of growing seedlings and lay eggs after 2 ‰ potassium permanganate sterilizations, control in the cylinder of growing seedlings water temperature at 20 ℃.The leech mating also pierces in cylinder a side middle product cocoon that loosens the soil.Leech lays eggs and starts to artificially collect in latter about 10 days.Get the ovum cocoon of giving birth in 3 days and be emitted on the loose moisturizing earth surfaces of incubator bottom in batches, then on the ovum cocoon, cover 2 layers of gauze, cover the soft earth of the about 1cm of a layer thickness again on gauze, the incubator side is with air-vent.Finally incubator is concentrated and put into climatic chamber, temperature is controlled at 25 ℃ of left and right, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH.Through left and right leech hatching in a week.Now leech seedling body weight is between the 0.05-0.08g/ bar, and natural situation lower body length is between 0.4-0.8cm.
2.3 leech seedling fluid openings feed preparation: 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04% B B-complex (w/v), 10g blood meal and 5g saccharomycete.Mentioned component is fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.According to above-mentioned formula rate according to the experiment actual needs, matching while using.
2.4 the leech seedling is fed: breeding experiment carries out University Of Suzhou's Experiment of Zoology is indoor.The wide-aperture sponge of 300 * 250 * 30mm specification is immersed in the fluid openings feed to 1 hour, allows sponge fully receive liquid feed.By the leech seedling that just hatches out, according to 500,1000,1500 and 2000 of density arrange four levels be put into respectively the bottom be placed with in the cylinder of growing seedlings of sponge.And evening allow leech suck on sponge each 2 hours at 7 at 11 in the morning every day respectively, now adopts water spray means to keep growing seedlings cylinder humidity.Hide gauze in order to avoid leech escapes above the cylinder of growing seedlings.Every group of 3 repetitions of seedling.Each leech sucks rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with 80 order nylon mesh screens, leech is fished for immediately, discards salt solution, and sponge is clean standby.The leech of pulling out moves to respectively in the cylinder of growing seedlings that depth of water 15cm is housed in addition.The grow seedlings top of cylinder is provided with anti-escape net, and cobble is put into as base in bottom.Control is grown seedlings the cylinder water temperature 25 ℃ of left and right, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour.Repeat subsequently above-mentioned feeding process every day, observed and recorded leech upgrowth situation, reject dead leech in time.
2.5 got at random respectively 100 every 5 days from each test group, weigh, the statistics survival rate.Change fresh liquid feed in sponge every day.
2.6 statistical method
Adopt SPSS 19.0 software kits to use minimum significantly range method (LSR, Least significant ranges) to carry out the statistical comparison analysis to leech density and body weight, finally be combined into motility rate and leech seedling body weight is carried out analysis-by-synthesis.
3 results and analysis
3.1 different leech seedling stocking densities are on its growth body weight impact
Table 1, different leech density are on its body weight impact
Different leech Density on Growth body weight impacts are comparatively obvious as can be seen from Table 1.Under certain density, through 20 days employing liquid feeds, to feed, the leech growth of seedling is very fast.Except density be 2000 average whose body weights of experimental group 0.16g, all the other are respectively organized average whose body weight and all reach the 0.28g/ bar, and through the check significant difference.
3.2 the impact of different leech seedling stocking densities on its survival rate
The impact of the different leech seedling of table 2 stocking density on its survival rate
Different leech seedling stocking densities have considerable influence to its survival rate as can be seen from Table 2.In identical raising situation, starting in 10 days, its survival rate increases and reduces to some extent along with density, and when wherein density is 2000, survival rate is down to 82%, and with other each group, all there were significant differences; In in the end 10 days, the leech survival rate can reach more than 99%, and this explanation leech seedling grows gradually, and its resistance against diseases increases.Therefore in leech seedling initial stage feeding process, when density surpasses 2000, because leech causes survival rate to descend to flood competition.
3.4 comprehensive, pass judgment on
From the experimental data of table 1 and table 2, can find out, under the identical condition of feeding, the leech speed of growth reduces along with the increase of density, when density is 2000, its early stage leech seedling percent significantly descends, and individual average weight minimum, with other, respectively organizes significant difference.Therefore, consider the composite factors such as economic benefit, should select leech density is 1500, uses the feeding method of aforesaid liquid starter diet can reach the quality and quantity that best commodity are used seedling.
The liquid feed of the leech seedling of the embodiment of the present invention and use this liquid feed to raise the method for leech seedling, can make seedling to young seedling shorten to 20 days growth period, average whose body weight is in 0.28g/ bar left and right, and survival rate remains on more than 96%, use the feeding method of liquid feed of the present invention can reach the best commodity quality and quantity with seedling.
Below for creator of the present invention within the concrete time experiment illustrate.
This is tested on March 5th, 2011 and starts to carry out indoor concentrated hatching in University Of Suzhou's Experiment of Zoology chamber.Selective body focuses on 300 of the physically fit adult kind leech of 18-22g/ bar.Put into the glass cylinder of growing seedlings and lay eggs after 2 ‰ potassium permanganate sterilizations, control in the cylinder of growing seedlings water temperature at 20 ℃.The leech mating also pierces in cylinder a side middle product cocoon that loosens the soil.April 1, leech started to lay eggs successively.Environment now needs peace and quiet.Leech is digged after laying eggs 10 days and collects the ovum cocoon, and attention can not the interior embryonated egg of squeeze and destroy cocoon.April 10 started to get the ovum cocoon of giving birth in 3 days and be emitted on the loose moisturizing earth surfaces of incubator bottom in batches, then on the ovum cocoon, cover 2 layers of gauze, cover the soft earth of the about 1cm of a layer thickness again on gauze, the incubator side is with air-vent.Finally incubator is concentrated and put into climatic chamber, temperature is controlled at 25 ℃ of left and right, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH.Successively obtain 570 ovum cocoons, started to hatch through one week left and right time.Collect just hatching seedling out, obtain altogether 6800 leech seedling.Through sampling Detection leech seedling body weight at the 0.059-0.078g/ bar, at the long 0.42-0.78cm of natural situation lower body.
The preparation of leech seedling liquid feed and feeding method: the preparation of leech seedling fluid openings feed, according to following formula rate preparation, is got 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04% B B-complex (w/v), 10g blood meal and 5g saccharomycete.Mentioned component is fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.Liquid feed according to actual needs, matching while using.April 8, breeding experiment carried out University Of Suzhou's Experiment of Zoology is indoor.The wide-aperture sponge of 300 * 250 * 30mm specification is immersed in the fluid openings feed to 1 hour, allows sponge fully receive liquid feed.By the leech seedling that just hatches out, be put into respectively bottom according to the density of the 1500/cylinder of growing seedlings and be placed with in the cylinder of growing seedlings of sponge.And evening allow leech suck on sponge each 2 hours at 7 at 11 in the morning every day respectively, now adopts water spray means to keep growing seedlings cylinder humidity.Hide gauze in order to avoid leech escapes above the cylinder of growing seedlings.Each leech sucks rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with 80 order nylon mesh screens, leech is fished for immediately, discards salt solution, and sponge is clean standby.The leech of pulling out moves to respectively in the cylinder of growing seedlings that depth of water 15cm is housed in addition.The grow seedlings top of cylinder is provided with anti-escape net, and cobble is put into as base in bottom.Control is grown seedlings the cylinder water temperature 25 ℃ of left and right, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour.
In seedling raising process, repeat above-mentioned feeding process every day, observed and recorded leech upgrowth situation, as find the thin out or hardening of leech seedling health color, can use 0.2% terramycin or 0.4% sulphoamidine azoles to admix liquid feed and prevent and treat enterogastritis and die back disease etc., as found, dead leech should reject in time.
Through the nursing of 20 days, detected the leech seedling percent more than 96% April 29, average whose body weight reaches the bar at 0.28 ± 0.054g/, at the long 0.44-0.75cm of natural situation lower body.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned example embodiment, and in the situation that do not deviate from spirit of the present invention or essential characteristic, can realize the present invention with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, therefore is intended to include in the present invention dropping on the implication that is equal to important document of claim and all changes in scope.Any Reference numeral in claim should be considered as limit related claim.
In addition, be to be understood that, although this specification is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should make specification as a whole, and the technical scheme in each embodiment also can, through appropriate combination, form other embodiments that it will be appreciated by those skilled in the art that.
Claims (9)
1. the liquid feed of a leech seedling, it is characterized in that: described liquid feed is comprised of 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal, 3-7g saccharomycete and distilled water 500ml.
2. the liquid feed of leech seedling according to claim 1, it is characterized in that: described liquid feed is comprised of 250ml cow's serum, 50g raw egg, 30g glucose, 20g dregs of beans, 1.5g calcium carbonate, 0.04%w/v B B-complex, 10g blood meal, 5g saccharomycete and distilled water 500ml.
3. the collocation method of the liquid feed of a leech seedling, it is characterized in that: the method is specially: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal and 3-7g saccharomycete are fully mixed to rear adding distil water to 500ml, and adjust pH is 7.0.
4. the method for breeding of a leech seedling, it is characterized in that: this method for breeding right to use requires 1 described liquid feed, and this method for breeding comprises the steps:
(1) hatching of leech seedling:
A1, selective body focus on the physically fit adult kind leech of 18-22g/ bar, put into the glass cylinder of growing seedlings and lay eggs after sterilization, control in the cylinder of growing seedlings water temperature at 20 ℃, and the leech mating also pierces in cylinder a side middle product cocoon that loosens the soil;
A2, the leech rear 9-11 days of laying eggs starts to artificially collect, and gets the ovum cocoon of giving birth in 3 days and is emitted on the loose moisturizing earth surfaces of an incubator bottom, and the incubator side, with air-vent, covers 2 layers of gauze on the ovum cocoon, covers the soft earth of one deck on gauze again;
A3, incubator is concentrated and to be put into a climatic chamber, temperature is controlled at 24-26 ℃, and the humidity of earth is controlled at 60%RH, and in case, relative air humidity remains on 80-85%RH;
A4,5-10 days leech seedling of process hatch out;
(2) the leech seedling raises and train
B1, the wide-aperture sponge of 300 * 250 * 30mm specification is immersed in liquid feed to 1 hour, allows sponge fully receive liquid feed;
B2, the leech seedling that will just hatch out are put into according to constant density glass that bottom is placed with sponge and grow seedlings in cylinder, and hide gauze in order to avoid the leech seedling escapes above the cylinder of growing seedlings;
B3, every day and evening allow the leech seedling suck on sponge each 2 hours at 7 at 11 respectively in the morning, and adopt water spray means to keep growing seedlings cylinder humidity;
B4, each leech suck rear use 30% salt solution thorn goads leech into action and all brushes down from the sponge upper punch, then with the nylon mesh screen, the leech seedling is fished for out immediately, discards salt solution, and sponge is clean standby;
B5, the leech of pulling out move to respectively in the cylinder of growing seedlings that depth of water 15cm is housed in addition, and the top of the cylinder of growing seedlings is provided with anti-escape net, and cobble is put into as base in bottom, 10 of every mornings and 8 oxygenations in evening 2 times, each 0.5 hour;
Repeat B6, every day the feeding process of B3-B5, change the liquid feed in sponge every day.
5. the method for breeding of leech seedling according to claim 4, it is characterized in that: in described step B2, the density of leech seedling is 1500.
6. the method for breeding of leech seedling according to claim 4 is characterized in that: the leech seedling that described method for breeding is raised is eurysome golden thread leech.
7. the method for breeding of leech seedling according to claim 4 is characterized in that: the described glass cylinder specification of growing seedlings is 40 * 30 * 20cm.
8. the method for breeding of leech seedling according to claim 4, it is characterized in that: the nylon mesh screen in described step B4 is 80 order nylon mesh screens.
9. the method for breeding of leech seedling according to claim 4, it is characterized in that: in described step B5, water body pH is between 6～7, and water temperature remains between 20-30 ℃, and dissolved oxygen amount is greater than 8 mg/litre.
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