CN102919180A - Artificial breeding method of sepia esculenta - Google Patents
Artificial breeding method of sepia esculenta Download PDFInfo
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- CN102919180A CN102919180A CN2012104745423A CN201210474542A CN102919180A CN 102919180 A CN102919180 A CN 102919180A CN 2012104745423 A CN2012104745423 A CN 2012104745423A CN 201210474542 A CN201210474542 A CN 201210474542A CN 102919180 A CN102919180 A CN 102919180A
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- 241001441745 Sepia esculenta Species 0.000 title claims abstract description 67
- 238000009395 breeding Methods 0.000 title abstract 3
- 235000012054 meals Nutrition 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000009278 visceral effect Effects 0.000 claims abstract description 15
- 230000012447 hatching Effects 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 235000013601 eggs Nutrition 0.000 claims description 20
- 241000238366 Cephalopoda Species 0.000 claims description 14
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 14
- 239000011707 mineral Substances 0.000 claims description 14
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 10
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 10
- 244000105624 Arachis hypogaea Species 0.000 claims description 10
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 10
- 235000018262 Arachis monticola Nutrition 0.000 claims description 10
- 241000238557 Decapoda Species 0.000 claims description 10
- 235000019733 Fish meal Nutrition 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 235000021323 fish oil Nutrition 0.000 claims description 10
- 239000004467 fishmeal Substances 0.000 claims description 10
- 235000013312 flour Nutrition 0.000 claims description 10
- 235000020232 peanut Nutrition 0.000 claims description 10
- 229940083466 soybean lecithin Drugs 0.000 claims description 10
- 229940088594 vitamin Drugs 0.000 claims description 10
- 229930003231 vitamin Natural products 0.000 claims description 10
- 235000013343 vitamin Nutrition 0.000 claims description 10
- 239000011782 vitamin Substances 0.000 claims description 10
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 10
- 239000013535 sea water Substances 0.000 claims description 9
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 6
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 230000001418 larval effect Effects 0.000 claims description 6
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 claims description 4
- 229960005069 calcium Drugs 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 210000002149 gonad Anatomy 0.000 claims description 3
- 230000029052 metamorphosis Effects 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 6
- 235000019784 crude fat Nutrition 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 3
- 241000238371 Sepiidae Species 0.000 abstract description 2
- 238000009364 mariculture Methods 0.000 abstract description 2
- 235000019750 Crude protein Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000004602 germ cell Anatomy 0.000 abstract 1
- 239000000463 material Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000707 wrist Anatomy 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000252254 Catostomidae Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- VQLYBLABXAHUDN-UHFFFAOYSA-N bis(4-fluorophenyl)-methyl-(1,2,4-triazol-1-ylmethyl)silane;methyl n-(1h-benzimidazol-2-yl)carbamate Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1.C=1C=C(F)C=CC=1[Si](C=1C=CC(F)=CC=1)(C)CN1C=NC=N1 VQLYBLABXAHUDN-UHFFFAOYSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Fodder In General (AREA)
Abstract
An artificial breeding method of sepia esculenta belongs to the technical field of mariculture. The method is characterized by comprising the following steps: 1, selection and matching of parents; 2, strengthened culturing of the parents; 3, spawning and hatching; 4, cultivation of larvae; and 5, cultivation of young sepia esculenta. The artificial breeding method of sepia esculenta is simple in step and high in operability; the feeding amount of the sepia esculenta parents is regulated and controlled manually, particularly the sepia esculenta parents are mainly fed with fly maggots, wherein the fly maggot powder has high protein content being 56%-63%, fat content being 13%, ash content being 7%, sugar content being 3.1%, crude protein content being 15% and crude fat being 5.8%, so nutritional requirement of growth of the sepia esculenta parents can be met and thus a batch of high-quality germ cells are obtained; and then the feeding amount and the like of offspring seeds of sepia esculenta are regulated and controlled manually, and especially bait contains cuttlefish visceral meal, so the growth speed of offspring seeds of sepia esculenta can be increased and the offspring seeds are stably supplied in batches.
Description
Technical field
The invention belongs to the mariculture technology field, be specifically related to a kind of golden cuttlefish artificial raise seedling method.
Background technology
Golden cuttlefish has another name called inkfish, snakeheaded fish.Medium-sized cuttlefish, the metastomium oval, long 20 centimetres of general intestines, length is 1.5 times of width.Back of the body abdomen is slightly flat, and lateral margin winds with narrow fin, disunion.Around head front end, the mouth 5 pairs of wrists are arranged, 4 pairs shorter, and each dirty upper length has 4 suckers; 1 pair of tactile wrist slightly surpasses succeeds length, and its sucker is only on the top, and is small and dense.Eye is flourishing, and the calcium carbonate endoskeleton is flourishing, and oblong, length are about 2.5 times of width, and the rear end spicule is sturdy.Ink sac is arranged, interior contain black liquor in the body.The body yellowish-brown, taking has the thin spot of brown purple and white alternate on the body, and the Xiong Tiyin back of the body has wavy striped, is in the sun golden colour lustre.China coast all has distribution, with yellow, Bohai Sea output is more.Shandong Province's Rizhao City is main distribution centre, and term is many in 8～December, and be the best fishing period November.
Summary of the invention
For the problem that prior art exists, the object of the present invention is to provide a kind of technical scheme of golden cuttlefish artificial raise seedling method,, growth rate loaded down with trivial details with golden cuttlefish offspring seed cultivation method in the solution prior art waits problem slowly.
Described golden cuttlefish artificial raise seedling method is characterized in that the method may further comprise the steps:
1) selecting and combo of parent: according to the natural propagation method of golden cuttlefish, respectively screening health, sexually matured male and female golden cuttlefish do the parent, and then the male and female golden cuttlefish carries out combo by 1～2:1;
2) parent's hot housing: with the golden cuttlefish parent in the step 1), put into nursery pond and carried out hot housing 15～30 days; It is 20～25 ℃ that the pond water temperature is cultivated in control, seawater salinity 25～30, simultaneously golden cuttlefish is carried out the stimulation by running water 10～15 minutes of 5～8m/min, feeding volume was 0.3～0.6 times of parent's weight by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding in per 2 hours;
3) spawning and hatching: with above-mentioned confirm gonad maturity through the golden cuttlefish parent after the hot housing after, move in the spawning pond and lay eggs, in the pond, put into egg apparatus, and by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding, feeding volume is 1.2～1.5 times of seedling weight, day quantity of exchanged water 100%～300%, keeping cultivating the pond water temperature is 20～25 ℃, seawater salinity 25～30, and the water-soluble oxygen amount is more than or equal to 6mg/l, behind golden cuttlefish parent natural spawning, collect fertilized egg and hatch;
4) larval culture: the golden cuttlefish young after will hatching is put into to cultivate in the pond and cultivates, wherein the condition of larval culture is: water temperature is 20～25 ℃, seawater salinity 25～30, day quantity of exchanged water 30 %～100%, soil pick-up is 1～2 time at the bottom of day pond, intensity of illumination is 500LX～1000LX, bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, feeding volume is 1.5～2.0 times of seedling weight, and metamorphosis is the young body of golden cuttlefish to the young after 30～45 days through cultivating;
5) young body is cultivated: the young body of above-mentioned golden cuttlefish is put into the indoor pond that is hung with things concealed cultivate, wherein the condition of young body cultivation is: water temperature 22-26 ° of C, salinity 30～40, day quantity of exchanged water 100 %～200 %; Intensity of illumination is 500LX～1000LX, and bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, and feeding volume is 2.0～3.0 times of seedling weight; Treat that young volume density reaches 300～1000/m
3Divide the pond to cultivate.
Described golden cuttlefish artificial raise seedling method is characterized in that described step 2) and step 3) in the percentage by weight of fly maggot, vitamin, oppossum shrimp and mineral matter be 46:5:45:4.
Described golden cuttlefish artificial raise seedling method is characterized in that fish meal in described step 4) and the step 5), dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and biphosphate weight percent calcium are 34:19:6:3:8:24:4:1.1:0.9.
Described golden cuttlefish artificial raise seedling method is characterized in that described mineral matter is by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI be that 1～2:0.8～1.2:2～4:0.5～0.8 is mixed to get by weight.
Described golden cuttlefish artificial raise seedling method is characterized in that described egg apparatus is that diameter is plastic tube, during hatching, will put in the aquarium with the egg apparatus of fertilized egg, adopts flowing water, little inflation hatching; Wherein streamflow is 50%～150%.
Golden cuttlefish artificial raise seedling method of the present invention, step is simple, workable, by the artificial regulatory golden cuttlefish parent material of throwing something and feeding, Feeding time, feeding volume, Reproduction Conditions etc., particularly fly maggot is as the main component of the material of throwing something and feeding, the fly maggot powder gross protein value is up to 56%～63%(average out to 59.5%), fat 13%, ash content 7%, carbohydrate 3.1%, thick protein 15%, crude fat 5.8%, can satisfy the nutritional need of new this growth of golden cuttlefish, can obtain the fertilized egg of a collection of high-quality, then by the artificial regulatory golden cuttlefish seed material of throwing something and feeding, Feeding time, feeding volume, growth conditions etc., particularly squid visceral meal has experiment to show mutually nibbling between the golden cuttlefish, can increase its growth rate, therefore squid visceral meal can speed the growth rate of golden cuttlefish as the composition of bait, forms stable, seed in batches.
Embodiment
Further specify the present invention below in conjunction with embodiment.
Embodiment
The golden cuttlefish artificial raise seedling method, the method may further comprise the steps:
1) selecting and combo of parent: according to the natural propagation method of golden cuttlefish, respectively screening health, sexually matured male and female golden cuttlefish do the parent, and then the male and female golden cuttlefish carries out combo by 1～2:1;
2) parent's hot housing: with the golden cuttlefish parent in the step 1), put into nursery pond and carried out hot housing 15～30 days; It is 20～25 ℃ that the pond water temperature is cultivated in control, seawater salinity 25～30, simultaneously golden cuttlefish is carried out the stimulation by running water 10～15 minutes of 5～8m/min, feeding volume was 0.3～0.6 times of parent's weight by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding in per 2 hours; The percentage by weight of fly maggot, vitamin, oppossum shrimp and mineral matter is 46:5:45:4, and mineral matter is by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI be that 1～2:0.8～1.2:2～4:0.5～0.8 is mixed to get by weight;
3) spawning and hatching: with above-mentioned confirm gonad maturity through the golden cuttlefish parent after the hot housing after, move in the spawning pond and lay eggs, in the pond, put into egg apparatus, egg apparatus is plastic tube, during hatching, will put in the aquarium with the egg apparatus of fertilized egg, adopt flowing water, little inflation hatching; Wherein streamflow is 50%～150%, and by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding, feeding volume is 1.2～1.5 times of seedling weight, and the percentage by weight of fly maggot, vitamin, oppossum shrimp and mineral matter is 46:5:45:4, and mineral matter is by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI be that 1～2:0.8～1.2:2～4:0.5～0.8 is mixed to get by weight, day quantity of exchanged water 100%～300%, keeping cultivating the pond water temperature is 20～25 ℃, seawater salinity 25～30, the water-soluble oxygen amount, is collected fertilized egg and is hatched behind golden cuttlefish parent natural spawning more than or equal to 6mg/l;
4) larval culture: the golden cuttlefish young after will hatching is put into to cultivate in the pond and cultivates, wherein the condition of larval culture is: water temperature is 20～25 ℃, seawater salinity 25～30, day quantity of exchanged water 30 %～100%, soil pick-up is 1～2 time at the bottom of day pond, intensity of illumination is 500LX～1000LX, bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, feeding volume is 1.5～2.0 times of seedling weight, fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and biphosphate weight percent calcium are 34:19:6:3:8:24:4:1.1:0.9, and metamorphosis is the young body of golden cuttlefish to the young after 30～45 days through cultivating;
5) young body is cultivated: the young body of above-mentioned golden cuttlefish is put into the indoor pond that is hung with things concealed cultivate, wherein the condition of young body cultivation is: water temperature 22-26 ° of C, salinity 30～40, day quantity of exchanged water 100 %～200 %; Intensity of illumination is 500LX～1000LX, bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, feeding volume is 2.0～3.0 times of seedling weight, and fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and biphosphate weight percent calcium are 34:19:6:3:8:24:4:1.1:0.9; Treat that young volume density reaches 300～1000/m
3Divide the pond to cultivate.
Golden cuttlefish artificial raise seedling method of the present invention, step is simple, workable, by artificial regulatory golden cuttlefish parent throw something and feed material, Feeding time, feeding volume, Reproduction Conditions etc., particularly fly maggot is as the main component of the material of throwing something and feeding, the fly maggot powder gross protein value is up to 56%～63%(average out to 59.5%), fat 13%, ash content 7%, carbohydrate 3.1%, thick protein 15%, crude fat 5.8%, can satisfy the nutritional need of new this growth of golden cuttlefish, can obtain the fertilized egg of a collection of high-quality; Then by artificial regulatory golden cuttlefish seed throw something and feed material, Feeding time, feeding volume, growth conditions etc., squid visceral meal particularly, there is experiment to show, mutually nibbling between the golden cuttlefish, can increase its growth rate, therefore squid visceral meal can speed the growth rate of golden cuttlefish as the composition of bait, forms seed stable, in batches.
Claims (5)
1. golden cuttlefish artificial raise seedling method is characterized in that the method may further comprise the steps:
1) selecting and combo of parent: according to the natural propagation method of golden cuttlefish, respectively screening health, sexually matured male and female golden cuttlefish do the parent, and then the male and female golden cuttlefish carries out combo by 1～2:1;
2) parent's hot housing: with the golden cuttlefish parent in the step 1), put into nursery pond and carried out hot housing 15～30 days; It is 20～25 ℃ that the pond water temperature is cultivated in control, seawater salinity 25～30, simultaneously golden cuttlefish is carried out the stimulation by running water 10～15 minutes of 5～8m/min, feeding volume was 0.3～0.6 times of parent's weight by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding in per 2 hours;
3) spawning and hatching: with above-mentioned confirm gonad maturity through the golden cuttlefish parent after the hot housing after, move in the spawning pond and lay eggs, in the pond, put into egg apparatus, and by fly maggot, vitamin, oppossum shrimp and the mineral matter feed of alternately throwing something and feeding, feeding volume is 1.2～1.5 times of seedling weight, day quantity of exchanged water 100%～300%, keeping cultivating the pond water temperature is 20～25 ℃, seawater salinity 25～30, and the water-soluble oxygen amount is more than or equal to 6mg/l, behind golden cuttlefish parent natural spawning, collect fertilized egg and hatch;
4) larval culture: the golden cuttlefish young after will hatching is put into to cultivate in the pond and cultivates, wherein the condition of larval culture is: water temperature is 20～25 ℃, seawater salinity 25～30, day quantity of exchanged water 30 %～100%, soil pick-up is 1～2 time at the bottom of day pond, intensity of illumination is 500LX～1000LX, bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, feeding volume is 1.5～2.0 times of seedling weight, and metamorphosis is the young body of golden cuttlefish to the young after 30～45 days through cultivating;
5) young body is cultivated: the young body of above-mentioned golden cuttlefish is put into the indoor pond that is hung with things concealed cultivate, wherein the condition of young body cultivation is: water temperature 22-26 ° of C, salinity 30～40, day quantity of exchanged water 100 %～200 %; Intensity of illumination is 500LX～1000LX, and bait is fish meal, dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and calcium dihydrogen phosphate, and feeding volume is 2.0～3.0 times of seedling weight; Treat that young volume density reaches 300～1000/m
3Divide the pond to cultivate.
2. golden cuttlefish artificial raise seedling method according to claim 1 is characterized in that described step 2) and step 3) in the percentage by weight of fly maggot, vitamin, oppossum shrimp and mineral matter be 46:5:45:4.
3. golden cuttlefish artificial raise seedling method according to claim 1 is characterized in that fish meal in described step 4) and the step 5), dregs of beans, yeast, squid visceral meal, peanut meal, Strong flour, fish oil, soybean lecithin and biphosphate weight percent calcium are 34:19:6:3:8:24:4:1.1:0.9.
4. golden cuttlefish artificial raise seedling method according to claim 1 and 2 is characterized in that described mineral matter is by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI be that 1～2:0.8～1.2:2～4:0.5～0.8 is mixed to get by weight.
5. golden cuttlefish artificial raise seedling method according to claim 1 is characterized in that described egg apparatus is that diameter is plastic tube, during hatching, will put in the aquarium with the egg apparatus of fertilized egg, adopts flowing water, little inflation hatching; Wherein streamflow is 50%～150%.
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Cited By (5)
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| CN103222435A (en) * | 2013-04-28 | 2013-07-31 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103548721A (en) * | 2013-10-17 | 2014-02-05 | 宁波大学 | Method for cultivating large-size offspring seeds of pharaoh cuttlefish |
| CN108935257A (en) * | 2018-09-22 | 2018-12-07 | 淮海工学院 | A kind of shrimp ponds net cage combined breeding method |
| CN109362610A (en) * | 2018-03-22 | 2019-02-22 | 中国水产科学研究院黄海水产研究所 | Method for cultivating fry of sepia reiliana |
| CN109757406A (en) * | 2019-02-27 | 2019-05-17 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Golden cuttlefish seed enrichment procedure |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222435A (en) * | 2013-04-28 | 2013-07-31 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103222435B (en) * | 2013-04-28 | 2014-07-16 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103548721A (en) * | 2013-10-17 | 2014-02-05 | 宁波大学 | Method for cultivating large-size offspring seeds of pharaoh cuttlefish |
| CN109362610A (en) * | 2018-03-22 | 2019-02-22 | 中国水产科学研究院黄海水产研究所 | Method for cultivating fry of sepia reiliana |
| CN109362610B (en) * | 2018-03-22 | 2021-06-25 | 中国水产科学研究院黄海水产研究所 | Method for cultivating fry of sepia reiliana |
| CN108935257A (en) * | 2018-09-22 | 2018-12-07 | 淮海工学院 | A kind of shrimp ponds net cage combined breeding method |
| CN109757406A (en) * | 2019-02-27 | 2019-05-17 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Golden cuttlefish seed enrichment procedure |
| CN109757406B (en) * | 2019-02-27 | 2021-11-05 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Sepia esculenta fry proliferation method |
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