CN101703013A - Biological engineering method of squids - Google Patents
Biological engineering method of squids Download PDFInfo
- Publication number
- CN101703013A CN101703013A CN200910154218A CN200910154218A CN101703013A CN 101703013 A CN101703013 A CN 101703013A CN 200910154218 A CN200910154218 A CN 200910154218A CN 200910154218 A CN200910154218 A CN 200910154218A CN 101703013 A CN101703013 A CN 101703013A
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- China
- Prior art keywords
- spermatophore
- biological engineering
- squids
- engineering method
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000238366 Cephalopoda Species 0.000 title claims abstract description 21
- 238000012407 engineering method Methods 0.000 title claims abstract description 16
- 239000013535 sea water Substances 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 241000238371 Sepiidae Species 0.000 claims description 24
- 241001663378 Sepiella maindroni Species 0.000 claims description 16
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 12
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 7
- 229960005323 phenoxyethanol Drugs 0.000 claims description 7
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 6
- 239000005770 Eugenol Substances 0.000 claims description 6
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 6
- 229960002217 eugenol Drugs 0.000 claims description 6
- 159000000000 sodium salts Chemical class 0.000 claims description 6
- 239000007943 implant Substances 0.000 claims description 5
- 238000005273 aeration Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims 1
- 230000009027 insemination Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 206010067130 Spastic diplegia Diseases 0.000 abstract 1
- 206010008129 cerebral palsy Diseases 0.000 abstract 1
- 230000012447 hatching Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 241001441745 Sepia esculenta Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a biological engineering method of squids, which comprises the steps of selecting a masculine adult squid, pinching off spermatophore thereof, and immersing the spermatophore in low-temperature seawater containing adapting reagent; putting a feminine squid in a container filled with low-temperature seawater for temporary culture for 5 to 30 minutes; charging CO2 into the low-temperature seawater; taking the feminine squid out, and implanting the spermatophore into a mantle of the feminine squid; putting the feminine squid after insemination back to the container filled with low-temperature water, charging CO2 into the seawater and boosting pressure; reducing pressure to normal, and then increasing the temperature of water gradually. The spermatophore is immersed in the low-temperature seawater containing the adapting reagent, and after insemination, failures caused by pinch-off of the spermatophore by a tentacle of a female parent due to inadaptation to the spermatophore can be effectively reduced. The biological engineering method of squids is applicable to biological engineering operation of homologous or heterogenetic squids, has the advantages of high hatching rate, high survival rate, little disease, easy culture, strong disease resistance, rapid growth and the like, and the biological engineering method has easy implementation and high success rate.
Description
Technical field
The present invention relates to a kind of siphonopods manual method, relate in particular to biological engineering method of squids.
Background technology
Sepiella maindroni once was one of the four big fishery in China fishing ground, and was stronger to the adaptability of temperature, in China coastal distribution very wide, be the very high traditional fishery kind of a kind of economic worth.Because overfishing and ecological degeneration since the seventies in 20th century, decay successively occurred.Last century, the eighties had the Primary Study to Sepiella maindroni resource and propagation such as report Li Xing a word used in person's names, and abroad had the biology of merchant cuttlefish and the report of breed aspect.In carrying out the domesticating and cultivating process of wild Sepiella maindroni, the technical staff finds that to the three generations, wild cuttlefish begins to adapt to the artificial domestication environment at domesticating and cultivating two, thereby can reach the purpose of large-scale production.Yet second or the cuttlefish of the third generation begin to occur the phenomenon that germplasms such as individuality reduces, premunition difference are degenerated.Especially the three generations is later propagates cuttlefish artificially, and its individual one can only reach half of wild individuality, has had a strong impact on market value.Therefore, press for and improve the quality of culturing cuttlefish, to be fit to industrial aquaculture.
Summary of the invention
The objective of the invention is to proterties, provide a kind of biological engineering method of squids, and this method is easy to implement, success rate is high with biological engineering's operation that can be applicable to of the same race or variety classes cuttlefish at the improvement cuttlefish.
Biological engineering method of squids, it has following steps:
1. choose ripe male cuttlefish, win spermatophore, and spermatophore is immersed in contains in the low temperature seawater that adapts to reagent;
2. choose female cuttlefish, put into the container that low temperature seawater is housed and supported 5 minutes to 30 minutes temporarily; Be filled with CO in the low temperature seawater
2
3. will 2. handle the female cuttlefish in back through step and take out, and will implant in the outer embrane of female cuttlefish through the spermatophore that 1. step is handled;
4. the female Sepiella maindroni after will inseminating is put back in the container of low temperature seawater CO
2Inflation, and supercharging;
5. air pressure is reduced to normal pressure, water temperature then progressively raises.
For optimizing technique scheme, the measure that the present invention taked also comprises:
Step 4. in, CO
2Aeration quantity be that 300mL/ (minL) is to 400mL/ (minL).
Adapt to sodium salt or 2-Phenoxyethanol that reagent contains eugenol;
The concentration of the sodium salt of eugenol in water body is 40mg/L to 100mg/L; The concentration of 2-Phenoxyethanol in water body is 100mL/L to 500mL/L;
The water temperature of low temperature seawater is 1 ℃ to 7 ℃.
Step 4. in, the pressure of supercharging is 1.1 atmospheric pressure to 1.5 atmospheric pressure, supercharging mode adopts the gradient supercharging.
The mode of the water temperature that progressively raises is for heating up continuously or gradient increased temperature; Temperature difference between the gradient of gradient increased temperature is less than 7 ℃.
Compared with prior art, the invention has the advantages that in the artificial breeding process of cuttlefish, to have adopted and win male cuttlefish spermatophore and originate as sperm, implant female cuttlefish and carry out breeding production operation, and in conjunction with crymoanesthesia, can effectively reduce the stress reaction of female cuttlefish, reduced the difficulty of operation. and, spermatophore is immersed in and contains in the low temperature seawater that adapts to reagent, after the insemination operation, can reduce female cuttlefish to a certain extent owing to the incompatibility of spermatophore is taken the situation that spermatophore causes operation failure with tentacle. in water body, charge into CO
2, the effect that can play the anesthesia of assisted cryogenic seawater also has the effect of certain inhibition bacterium, prevents bacterial infection.This method is particularly useful for biological engineering's operation of variety classes cuttlefish, adopt the resulting hybrid seedlings of this method, owing to have parent's different advantages concurrently, have incubation rate height, survival rate height, disease few, be easy to culture, advantage such as premunition is strong, growth is fast, be particularly useful for the crossover operation of variety classes cuttlefish.And the good shape of the seed of using this method to produce is fit to industrial aquaculture very much.
Embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment 1: as male parent, operate as female parent by female Sepiella maindroni with male golden cuttlefish for present embodiment.Biological engineering's method, it has following steps:
1. choose ripe male golden cuttlefish, win spermatophore, and spermatophore is immersed in contains in the low temperature seawater that adapts to reagent; The water temperature of low temperature seawater is 6 ℃.Adapting to reagent is the sodium salt of eugenol; The concentration of the sodium salt of eugenol in water body is 80mg/L.This operation is to make the spermatophore surface infiltration adapt to reagent, when treating follow-up insemination operation, adapts to reagent and can prevent that spermatophore from coming off, improving the success rate of insemination operation.
2. female Sepiella maindroni is put into the container that low temperature seawater is housed and was supported 15 minutes temporarily; Be filled with CO in the low temperature seawater
2Mainly be to make female Sepiella maindroni enter resting state, breathe this moment and slow down, do not move about that cuttlefish stress behavior hinder operation in the time of preventing to be fertilized operation.
3. will 2. handle the female Sepiella maindroni in back through step and take out, and will implant in the outer embrane of female Sepiella maindroni through the spermatophore that 1. step is handled;
4. the female Sepiella maindroni after will inseminating is put back in the container of low temperature seawater CO
2Inflation, and supercharging; CO
2Aeration quantity be 400mL/ (minL).The pressure of supercharging is 1.2 atmospheric pressure, and supercharging mode adopts the gradient supercharging.CO
2Play and suppress bacterium and anesthetic effect, supercharging can strengthen CO
2Dissolving, make female cuttlefish adapt to the spermatophore of implanting, improve the success rate of insemination operation.
5. air pressure is reduced to normal pressure, water temperature then progressively raises.The mode of the water temperature that progressively raises promptly adopts Electric heating that water temperature is raise gradually for heating up continuously.Behind the EO, female Sepiella maindroni put into breed the pond and raise and be provided with the ovum hapteron and wait for that it lays eggs.
Embodiment 2: biological engineering method of squids, and it has following steps:
1. choose ripe male golden cuttlefish, win spermatophore, and spermatophore is immersed in contains in the low temperature seawater that adapts to reagent; The water temperature of low temperature seawater is 7 ℃.Adapting to reagent is the 2-Phenoxyethanol; The concentration of 2-Phenoxyethanol in water body is 500mL/L, and promptly 2-Phenoxyethanol and water 1 to 1 mix.
2. female Sepiella maindroni is put into the container that low temperature seawater is housed and was supported 10 minutes temporarily; Be filled with CO in the low temperature seawater
2
3. will 2. handle the female Sepiella maindroni in back through step and take out, and will implant in the outer embrane of female Sepiella maindroni through the spermatophore that 1. step is handled;
4. the female Sepiella maindroni after will inseminating is put back in the container of low temperature seawater, CO
2Inflation, and supercharging; CO
2Aeration quantity be 400mL/ (minL).The pressure of supercharging is 1.1 atmospheric pressure, and supercharging mode adopts the gradient supercharging, adopts 3 uniform gradients to carry out the gradient supercharging, and pressurization time was controlled at about 20 minutes.
5. air pressure is reduced to normal pressure, water temperature then progressively raises. and the mode of the water temperature that progressively raises is a gradient increased temperature; Temperature difference between the gradient of gradient increased temperature is 2 ℃.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.
Claims (7)
1. biological engineering method of squids is characterized in that having following steps:
1. choose ripe male cuttlefish, win spermatophore, and spermatophore is immersed in contains in the low temperature seawater that adapts to reagent;
2. choose female cuttlefish, put into the container that low temperature seawater is housed and supported 5 minutes to 30 minutes temporarily; Be filled with CO in the low temperature seawater
2
3. will 2. handle the female cuttlefish in back through step and take out, and will implant in the outer embrane of female cuttlefish through the spermatophore that 1. step is handled;
4. the female Sepiella maindroni after will inseminating is put back in the container of described low temperature seawater CO
2Inflation, and supercharging;
5. air pressure is reduced to normal pressure, water temperature then progressively raises.
2. biological engineering method of squids according to claim 1 is characterized in that: step 4. in, CO
2Aeration quantity be that 300mL/ (minL) is to 400mL/ (minL).
3. biological engineering method of squids according to claim 2 is characterized in that: described adaptation reagent contains the sodium salt or the 2-Phenoxyethanol of eugenol.
4. biological engineering method of squids according to claim 3 is characterized in that: the concentration of the sodium salt of described eugenol in water body is 40mg/L to 100mg/L; The concentration of described 2-Phenoxyethanol in water body is 100mL/L to 500mL/L.
5. according to claim 3 or 4 described biological engineering method of squids, it is characterized in that: the water temperature of described low temperature seawater is 1 ℃ to 7 ℃.
6. biological engineering method of squids according to claim 5 is characterized in that: step 4. in, the pressure of described supercharging is 1.1 atmospheric pressure to 1.5 atmospheric pressure, supercharging mode adopts the gradient supercharging.
7. biological engineering method of squids according to claim 6 is characterized in that: the mode of the described water temperature that progressively raises is for heating up continuously or gradient increased temperature; Temperature difference between the gradient of gradient increased temperature is less than 7 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101542181A CN101703013B (en) | 2009-11-17 | 2009-11-17 | Biological engineering method of squids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101542181A CN101703013B (en) | 2009-11-17 | 2009-11-17 | Biological engineering method of squids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101703013A true CN101703013A (en) | 2010-05-12 |
| CN101703013B CN101703013B (en) | 2012-01-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101542181A Expired - Fee Related CN101703013B (en) | 2009-11-17 | 2009-11-17 | Biological engineering method of squids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101703013B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919180A (en) * | 2012-11-21 | 2013-02-13 | 浙江海洋学院 | Artificial breeding method of sepia esculenta |
| CN103222435A (en) * | 2013-04-28 | 2013-07-31 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103478053A (en) * | 2013-10-15 | 2014-01-01 | 苏州市相城区盛胡特种养殖专业合作社 | Method for efficiently culturing Sepiella maindroni de Rochebrune by using nano silicon hydroxyapatite |
| CN107897051A (en) * | 2017-12-14 | 2018-04-13 | 浙江海洋大学 | A kind of monosex cultivation method for improving cuttlefish and forming rate |
-
2009
- 2009-11-17 CN CN2009101542181A patent/CN101703013B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919180A (en) * | 2012-11-21 | 2013-02-13 | 浙江海洋学院 | Artificial breeding method of sepia esculenta |
| CN102919180B (en) * | 2012-11-21 | 2014-06-04 | 浙江海洋学院 | Artificial breeding method of sepia esculenta |
| CN103222435A (en) * | 2013-04-28 | 2013-07-31 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103222435B (en) * | 2013-04-28 | 2014-07-16 | 中国水产科学研究院黄海水产研究所 | Breeding method for sepia esculenta of quick-growing strain |
| CN103478053A (en) * | 2013-10-15 | 2014-01-01 | 苏州市相城区盛胡特种养殖专业合作社 | Method for efficiently culturing Sepiella maindroni de Rochebrune by using nano silicon hydroxyapatite |
| CN107897051A (en) * | 2017-12-14 | 2018-04-13 | 浙江海洋大学 | A kind of monosex cultivation method for improving cuttlefish and forming rate |
| CN107897051B (en) * | 2017-12-14 | 2020-03-24 | 浙江海洋大学 | Unisexual culture method for improving cuttlefish culture rate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101703013B (en) | 2012-01-11 |
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Effective date of registration: 20160411 Address after: Lincheng Sports Road 316021 Zhejiang city of Zhoushan province No. 20 Branch Chong Park A9 building 4 floor Patentee after: Zhejiang Dahaiyang Science and Technology Co., Ltd. Address before: The sea of Dinghai District in Zhejiang province Zhoushan City Road 316000 No. 18 Patentee before: Zhejiang Ocean University |
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Granted publication date: 20120111 Termination date: 20181117 |