CN107897051A - A kind of monosex cultivation method for improving cuttlefish and forming rate - Google Patents
A kind of monosex cultivation method for improving cuttlefish and forming rate Download PDFInfo
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- CN107897051A CN107897051A CN201711337533.9A CN201711337533A CN107897051A CN 107897051 A CN107897051 A CN 107897051A CN 201711337533 A CN201711337533 A CN 201711337533A CN 107897051 A CN107897051 A CN 107897051A
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- 229960001699 ofloxacin Drugs 0.000 claims description 15
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- BJIOGJUNALELMI-UHFFFAOYSA-N isoeugenol Chemical class COC1=CC(C=CC)=CC=C1O BJIOGJUNALELMI-UHFFFAOYSA-N 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- YFGHCGITMMYXAQ-UHFFFAOYSA-N 2-[(diphenylmethyl)sulfinyl]acetamide Chemical compound C=1C=CC=CC=1C(S(=O)CC(=O)N)C1=CC=CC=C1 YFGHCGITMMYXAQ-UHFFFAOYSA-N 0.000 claims description 7
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- 235000013372 meat Nutrition 0.000 description 3
- UIOFUWFRIANQPC-JKIFEVAISA-N Floxacillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C1=C(C)ON=C1C1=C(F)C=CC=C1Cl UIOFUWFRIANQPC-JKIFEVAISA-N 0.000 description 2
- BJIOGJUNALELMI-ONEGZZNKSA-N Isoeugenol Natural products COC1=CC(\C=C\C)=CC=C1O BJIOGJUNALELMI-ONEGZZNKSA-N 0.000 description 2
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- 239000013589 supplement Substances 0.000 description 2
- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
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- 108010054814 DNA Gyrase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001663395 Sepiella Species 0.000 description 1
- 241000472272 Sepiida Species 0.000 description 1
- 241000472271 Sepiolida Species 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
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- 229960002819 diprophylline Drugs 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- KSCFJBIXMNOVSH-UHFFFAOYSA-N dyphylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1N(CC(O)CO)C=N2 KSCFJBIXMNOVSH-UHFFFAOYSA-N 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of monosex cultivation method for improving cuttlefish and forming rate, specifically comprise the following steps：Select the cuttlefish of sexual gland early development；By cuttlefish deep anaesthesia；Anesthesia cuttlefish is taken out, gently pushes cuttlefish belly mantle cavity aside, electric light ear spoon or the similar end luminescent probe of self-control are inserted into outer embrane from the outside of belly, it is seen that bronzing spawn tangled gland is then female, it is seen that milky seminal vesicle device is then male；Cuttlefish after sorting is first rinsed with clear water, is then soaked with seawater, then cuttlefish mantle cavity is rinsed repeatedly with water pipe or tap, promotees its recovery；The cuttlefish of recovery is put back in clean seawater, normally cultivated.Have the beneficial effect that：Spouse's contention can be greatly reduced in monosex cultivation method breeding process of the present invention, fight and kill and devour problem, improve the survival rate in cuttlefish cultivation sexual maturing period；The cultural method can reduce the energy expenditure in reproductive process, the nutriment of absorption is mainly deposited in muscle, improve its yield per unit area.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly, to a kind of monosex cultivation side improved cuttlefish and form rate
Method.
Background technology
Sepiella maindroni（Sepiella maindroni）Belong to Mollusca Mollusca, Cephalopoda
Cephalopoda, Dibranchia Dibranchia, Sepiida Sepioidea, Sepiidae Sepiidae, sepiella maindroni de Rochebrune category
Sepiella.Sepiella maindroni is a kind of high protein, low fat, the more comprehensive marine product of nutrition.Its economic value is very
Height, its edible portion account for overall 92%.Its meat is pure white, full of nutrition, containing good protein and enriches micro member
Element, delicious flavour is often edible clever to prolong intelligence, anti-aging, improve hematopoiesis and immune function, to preventing cardiovascular disease
Disease, anaemia and tumour have certain effect.But with to spawning colony surround and seize and Swing net is to the damage of young cuttlefish
Evil, the increasing of catching intensity, prevents cuttlefish colony from obtaining effective supplement, along with the pollution and destruction of natural environment, from
Late 1980s start the drastically decline for Sepiella maindroni resource occur, and difficult formation is interrogated in fishing.Sepiella maindroni is
Just it is able to the important siphonopods species of propagation in scale and aquaculture exploitation recent years.
The siphonopods such as Sepiella maindroni, i.e., can be because of hero when i.e. by sexal maturity in the breeding process all there are a general character
Property between contention spouse problem occur it is fierce fight and kill and devour, preresearch estimates, it is this take the lead and kill and devour caused by loss and dead
Die the 60-70% for accounting for the whole forming stage death rate.Sometimes in one night the death rate with regard to 10-20% can be reached, this to cuttlefish cultivate and
Breed the influence of bringing on a disaster property of conservation.Monosex cultivation is to solve the problems, such as this good method, during monosex cultivation
There is no spouse's contention and problem of fighting, and is greatly improved the survival rate that cuttlefish cultivates sexual maturing period, but for a long time due to crow
Crafty male and female individual is difficult to distinguish in terms of shape, and batch isolating female from male culture is difficult to realize, and the meeting in male and female live body assorting room
Cuttlefish occurs stress ink-jet and mortality.Therefore there is an urgent need to establish a set of ripe monosex cultivation method.
The content of the invention
It is an object of the invention to provide a kind of cuttlefish batch male and female method for separating and operational procedure, monosex cultivation is established
Technology, greatly reduces and fights and kill and devour rate between cuttlefish sexual development period, the raising cuttlefish for improving the cultivation survival rate of cuttlefish is supported
Into the monosex cultivation method of rate.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical solution taken is：
It is a kind of to improve cuttlefish and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish recover,
Monosex cultivation, specifically comprises the following steps：
Sorting opportunity：The cuttlefish of sexual gland early development is selected, cuttlefish age size is the key factor of successful implementation monosex cultivation
One of, if the age is too small, sexual gland not yet develops the distinguishable degree of naked eyes, if the age is too big, is not easy to a point pond early and cultivates,
Aquaculture cost is increased, and manually-operated difficulty increased；
Cuttlefish is anaesthetized：Cuttlefish is put into the seawater of ethanol containing 1-3%, 17-18g/L magnesium chlorides and 3-6mg/L isoeugenols and is soaked
80-100s, makes cuttlefish deep anaesthesia, which can quickly deepen Sepiella maindroni general anesthesia, make Sepiella maindroni fast
Entering Anesthesia induction fastly, anesthesia maintenance time length reduces irritability, good condition has been supplied for the destruction of optic nerve, this
Outer isoeugenol and its metabolin can quickly be discharged from blood and tissue, and poisonous or mutation-ure will not be produced in body
Matter；
Method for separating：Anesthesia cuttlefish to be taken out, gently pushes cuttlefish belly mantle cavity aside, ovary and spermary are respectively positioned on the rear end of mantle cavity,
Electric light ear spoon or the similar end luminescent probe of self-control are inserted into outer embrane from the outside of belly, it is seen that bronzing spawn tangled gland is then female, is seen
It is then male to milky seminal vesicle device, cuttlefish male and female individual difference unobvious, and in shape changeable for body colour and figure
Mollusk, single accuracy rate of being distinguished from shape is not high, but when sexual gland germinates, spawn tangled gland and seminal vesicle device are then most typical
The distinguishing characteristic of male and female individual, the sorting be not required special instruments and equipment or reagent, once-through operation can complete sex abnormality and
Pond is divided to cultivate, identification accuracy rate may be up to 100%, can sort male and female cuttlefish in batches；
Cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, then uses modafinil containing 1-2ug/L and 0.2-0.4ug/L
Seawater immersion 10-20min, the during which oxygen in water >=12mg/L of Ofloxacin, then with water pipe or tap repeatedly
Cuttlefish mantle cavity is rinsed, promotees its recovery, the dextrorotation Ofloxacin containing 0.28-0.38%, Ofloxacin lead to wherein in Ofloxacin
Cross the activity for inhibiting bacteria DNA gyrases, prevent DNA of bacteria synthesis and duplication and cause bacterial death, there is broad-spectrum antiseptic
Effect, can avoid influence of the bacterium to cuttlefish wound, improve the survival rate of cuttlefish after sorting, and then improve supporting for monosex cultivation
Into rate, and dextrorotation Ofloxacin can be engaged with modafinil, can increase glutamine synthelase, promote the solution of nerve cell
Malicious function and energetic supersession activity and work, and then stimulate nervous centralis, enable anesthesia cuttlefish rapid recovery, reduce anesthesia
Injury to body, while promote the elimination of isoeugenol, improve the survival rate and safety in utilization of cuttlefish；
Monosex cultivation method：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater, after 4-6h
Feed frost small fish or shrimp is normally cultivated, feed the 8-12% that weight is cuttlefish gross weight, timing daily, fixed point are fed
2-4 times, spouse's contention can be greatly reduced in breeding process, fight and kill and devour problem, improve surviving for cuttlefish cultivation sexual maturing period
Rate, while the energy expenditure in reproductive process can be reduced, the nutriment of absorption is mainly deposited in muscle, increase edible portion
Point, meat and value are improved, improves its yield per unit area, aquaculture cost, increase culture benefit is reduced, peomotes cuttlefish
The fast development of aquaculture.
Compared with prior art, the advantage of the invention is that：It can subtract significantly in monosex cultivation method breeding process of the present invention
Few spouse's contention, fight and kill and devour problem, improves the survival rate in cuttlefish cultivation sexual maturing period；The cultural method can reduce reproduction
Energy expenditure in journey, makes the nutriment of absorption mainly be deposited in muscle, increases edible part, improves meat and value,
Improve its yield per unit area；Present invention anesthetic can make Sepiella maindroni promptly enter Anesthesia induction, anesthesia dimension
Time length is held, reduces irritability, can quickly be discharged from blood and tissue, poisonous or mutation-ure will not be produced in body
Matter；Special instruments and equipment or reagent is not required in method for separating of the present invention, and once-through operation can complete sex abnormality and a point pond is cultivated,
Identification accuracy rate may be up to 100%, can sort male and female cuttlefish in batches；Method for resuscitation of the present invention can enable anesthesia cuttlefish quickly revive
Wake up, reduce injury of the anesthesia to body, while promote the elimination of different anesthetic, improve the survival rate and safety in utilization of cuttlefish.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
It is a kind of to improve cuttlefish and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish recover,
Monosex cultivation, specifically comprises the following steps：
1）Sorting opportunity：Select the cuttlefish of sexual gland early development；
2）Cuttlefish is anaesthetized：Cuttlefish is put into the seawater containing 1% ethanol, 18g/L magnesium chlorides and 3mg/L isoeugenols and soaks 100s,
Make cuttlefish deep anaesthesia；
3）Method for separating：Anesthesia cuttlefish is taken out, cuttlefish belly mantle cavity is gently pushed aside, after ovary and spermary are respectively positioned on mantle cavity
End, outer embrane is inserted into by electric light ear spoon or the similar end luminescent probe of self-control from the outside of belly, it is seen that and bronzing spawn tangled gland is then female,
It is then male to see milky seminal vesicle device；
4）Cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, then uses modafinil containing 1ug/L and 0.4ug/L oxygen fluorine
Seawater the immersion 10min, during which oxygen in water >=12mg/L, then cuttlefish is rinsed repeatedly with water pipe or tap of Sha Xing
Mantle cavity, promotees its recovery, 0.38% dextrorotation Ofloxacin is contained wherein in Ofloxacin；
5）Monosex cultivation method：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater, after 4h
Feed frost small fish or shrimp is normally cultivated, feed the 8-12% that weight is cuttlefish gross weight, timing daily, fixed point are fed
2-4 times, until cuttlefish sexual maturing period, cuttlefish forms rate as 98.5%.
Embodiment 2：
It is a kind of to improve cuttlefish and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish recover,
Monosex cultivation, specifically comprises the following steps：
1）Sorting opportunity：Select the cuttlefish of sexual gland early development；
2）Cuttlefish is anaesthetized：Cuttlefish is put into the seawater containing 3% ethanol, 17g/L magnesium chlorides and 6mg/L isoeugenols and soaks 80s,
Make cuttlefish deep anaesthesia；
3）Method for separating：Anesthesia cuttlefish is taken out, cuttlefish belly mantle cavity is gently pushed aside, after ovary and spermary are respectively positioned on mantle cavity
End, outer embrane is inserted into by electric light ear spoon or the similar end luminescent probe of self-control from the outside of belly, it is seen that and bronzing spawn tangled gland is then female,
It is then male to see milky seminal vesicle device；
4）Cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, then uses modafinil containing 2ug/L and 0.2ug/L oxygen fluorine
Seawater the immersion 20min, during which oxygen in water >=12mg/L, then cuttlefish is rinsed repeatedly with water pipe or tap of Sha Xing
Mantle cavity, promotees its recovery, 0.28% dextrorotation Ofloxacin is contained wherein in Ofloxacin；
5）Monosex cultivation method：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater, 6h
After feed frost small fish or shrimp normally cultivated, feed weight be cuttlefish gross weight 8-12%, daily timing, fixed point throwing
Feed 2-4 times, until cuttlefish sexual maturing period, cuttlefish forms rate as 98.7%.
Embodiment 3：
It is a kind of to improve cuttlefish and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish recover,
Monosex cultivation, specifically comprises the following steps：
1）Sorting opportunity：Select the cuttlefish of sexual gland early development；
2）Cuttlefish is anaesthetized：Cuttlefish is put into the seawater containing 2% ethanol, 17.5g/L magnesium chlorides and 5mg/L isoeugenols and is soaked
90s, makes cuttlefish deep anaesthesia；
3）Method for separating：Anesthesia cuttlefish is taken out, cuttlefish belly mantle cavity is gently pushed aside, after ovary and spermary are respectively positioned on mantle cavity
End, outer embrane is inserted into by electric light ear spoon or the similar end luminescent probe of self-control from the outside of belly, it is seen that and bronzing spawn tangled gland is then female,
It is then male to see milky seminal vesicle device；
4）Cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, then uses modafinil containing 1.5ug/L and 0.3ug/L oxygen
Seawater the immersion 15min, during which oxygen in water >=12mg/L, then crow is rinsed repeatedly with water pipe or tap of Flucloxacillin
Crafty mantle cavity, promotees its recovery, 0.33% dextrorotation Ofloxacin is contained wherein in Ofloxacin；
5）Monosex cultivation method：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater, after 5h
Feed frost small fish or shrimp is normally cultivated, feed the 8-12% that weight is cuttlefish gross weight, timing daily, fixed point are fed
2-4 times, until cuttlefish sexual maturing period, cuttlefish forms rate as 99.3%.
Embodiment 4：
It is a kind of to improve cuttlefish and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish recover,
Monosex cultivation, specifically comprises the following steps：
1）Sorting opportunity：Select the cuttlefish of sexual gland early development；
2）Cuttlefish is anaesthetized：Cuttlefish is put into the dihydroxy containing 2% ethanol, 17.5g/L magnesium chlorides, 5mg/L isoeugenols and 0.02mg/L
90s is soaked in the seawater of third theophylline, makes cuttlefish deep anaesthesia, the addition of diprophylline can improve the cuttlefish in anesthesia
Respiration, assist anesthetic to reduce the irritability of cuttlefish, avoid generation stress ink-jet and mortality, drop to greatest extent
Low injury of the anesthesia to cuttlefish, while reduce the feeling of pain of cuttlefish so that cuttlefish recovery will not have excessive hot-tempered because of pain
Dynamic, that improves monosex cultivation forms rate；
3）Method for separating：Anesthesia cuttlefish is taken out, cuttlefish belly mantle cavity is gently pushed aside, after ovary and spermary are respectively positioned on mantle cavity
End, outer embrane is inserted into by electric light ear spoon or the similar end luminescent probe of self-control from the outside of belly, it is seen that and bronzing spawn tangled gland is then female,
It is then male to see milky seminal vesicle device；
4）Cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, then uses modafinil containing 1.5ug/L and 0.3ug/L oxygen
Seawater the immersion 15min, during which oxygen in water >=12mg/L, then crow is rinsed repeatedly with water pipe or tap of Flucloxacillin
Crafty mantle cavity, promotees its recovery, 0.33% dextrorotation Ofloxacin is contained wherein in Ofloxacin.
5）Monosex cultivation method：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater,
Frost small fish is fed after 5h or shrimp is normally cultivated, feeds the 8-12% that weight is cuttlefish gross weight, timing daily, fixed point
Feed 2-4 times, until cuttlefish sexual maturing period, cuttlefish forms rate as 99.8%.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (8)
- Improve cuttlefish 1. a kind of and form the monosex cultivation method of rate, including sorting opportunity, cuttlefish anesthesia, method for separating, cuttlefish are multiple Soviet Union, monosex cultivation, it is characterised in that：The cuttlefish recovers：Cuttlefish after sorting is first rinsed with clear water, is then soaked with seawater Bubble, is during which largely inflated, then rinses cuttlefish mantle cavity repeatedly with water pipe or tap, promotees its recovery.
- A kind of 2. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The cuttlefish Modafinil containing 1-2ug/L and 0.2-0.4ug/L Ofloxacins in seawater in recovery, soak 10-20min.
- A kind of 3. monosex cultivation method for improving cuttlefish and forming rate according to claim 2, it is characterised in that：The cuttlefish Dextrorotation Ofloxacin containing 0.28-0.38% in Ofloxacin in recovery.
- A kind of 4. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The cuttlefish Oxygen in water >=12mg/L during seawater immersion in recovery.
- A kind of 5. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The sorting Opportunity is：Select the cuttlefish of sexual gland early development.
- A kind of 6. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The cuttlefish Anaesthetize and be：Cuttlefish is put into the seawater of ethanol containing 1-3%, 17-18g/L magnesium chlorides and 3-6mg/L isoeugenols and soaks 80- 100s, makes cuttlefish deep anaesthesia.
- A kind of 7. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The sorting Method is：Anesthesia cuttlefish is taken out, gently pushes cuttlefish belly mantle cavity aside, ovary and spermary are respectively positioned on the rear end of mantle cavity, by electric light Ear spoon or the similar end luminescent probe of self-control are inserted into outer embrane from the outside of belly, it is seen that bronzing spawn tangled gland is then female, it is seen that milky white The seminal vesicle device of color is then male.
- A kind of 8. monosex cultivation method for improving cuttlefish and forming rate according to claim 1, it is characterised in that：The unisexuality Cultural method is：The cuttlefish of recovery is put back in clean seawater, 20-30/m of cultivation density2Underwater, feeds ice after 4-6h Freeze small fish or shrimp is normally cultivated, feed the 8-12% that weight is cuttlefish gross weight, timing daily, fixed point are fed 2-4 times.
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