The Sepiella maindroni expanding propagation method
Technical field
The invention belongs to the mariculture technology field, be specifically related to the Sepiella maindroni expanding propagation method.
Background technology
Sepiella maindroni be annual, feeding habits are violent, the rapidly ocean siphonopods of growing.Cuttlefish is distributed in coastal area of southeastern China, and Russia, Japan, Korea and Southeast Asia are coastal, and the sea area, Zhoushan is most important area.The cuttlefish meat flavour is delicious, and unique flavor is of high nutritive value, and deeply is subjected to liking of the consumer group.At present, the shortcomings such as the seed size of mass rearing is irregular, quantity is unstable, damage is large, poor operability can not form stable in batches high-quality seed production capacity.
Summary of the invention
Problem for prior art exists the object of the invention is to design the technical scheme that a kind of Sepiella maindroni expanding propagation method is provided.
Described Sepiella maindroni expanding propagation method is characterized in that comprising lower step:
1) parent's selects and combo
According to the natural propagation method of graceful formula sepiella maindroni de Rochebrune, respectively screening health, the graceful formula sepiella maindroni de Rochebrune of sexually matured male and female are done the parent, and then the graceful formula sepiella maindroni de Rochebrune of male and female carries out combo by 1~2:1;
2) parent supports temporarily
The graceful formula sepiella maindroni de Rochebrune that combo is good is supported temporarily in the indoor culture pond, it is 20~25 ℃ that the pond water temperature is cultivated in control, seawater salinity 25~30, photoperiod L:D is 4:20, simultaneously graceful formula sepiella maindroni de Rochebrune is carried out the stimulation by running water 10~15 minutes of 5~8m/min, feeding volume was 0.3~0.6 times of parent's weight by artemia larvae, vitamin, oppossum shrimp, the mineral matter feed of alternately throwing something and feeding in per 2 hours during supporting temporarily;
3) collect fertilized egg
Utilize the egg apparatus of cultivating in the pond to collect fertilized egg;
4) artificial incubation
Fertilized egg is positioned in the cultivation pond, and it is 20~25 ℃ that the pond water temperature is cultivated in control, seawater salinity 25~30, and the water-soluble oxygen amount is more than or equal to 6mg/l;
5) seedling culture
Begin the feed of throwing something and feeding in 6 hours behind the seedling incubation of membrane, the powder-material of front 5 days per 4 hours throw something and feed hairtail and Penaeus Vannmeis, feeding volume are 1.2~1.5 times of seedling weight; Threw mixed feed in per 6 hours after 5 days, feeding volume is 1.8~2.5 times of seedling weight, and described mixed feed is by artemia larvae: vitamin: oppossum shrimp: food starch: mineral matter: flavones is 2~4:0.5~0.8:2~4:1~2:0.4~0.8:0.2~0.5.
Described Sepiella maindroni expanding propagation method is characterized in that described step 2) in control to cultivate pool water level be 50~60cm.
Described Sepiella maindroni expanding propagation method is characterized in that described step 2) and the step 5) mineral by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI be that 1~2:0.8~1.2:2~4:0.5~0.8 is mixed to get by weight.
Described Sepiella maindroni expanding propagation method is characterized in that described step 2) in when water temperature is lower than 20 ℃, the induced spawning that progressively heats up, heating gradient are 0.5 ℃/day, until water temperature to 20~25 ℃.
Described Sepiella maindroni expanding propagation method is characterized in that control cultivation pool water level is 20~40cm in the described step 4).
Described Sepiella maindroni expanding propagation method is characterized in that food starch is barley starch, wheaten starch, corn starch or potato starch in the described step 5).
Above-mentioned Sepiella maindroni expanding propagation method, reasonable in design, step is simple, workable, by artificial regulatory Sepiella maindroni parent feeding volume, Feeding time, feeding volume, Reproduction Conditions etc., obtain the fertilized egg of a collection of high-quality, then by artificial regulatory Sepiella maindroni seed feeding volume, Feeding time, feeding volume, growth conditions etc., formed stable, seed in batches, by method of the present invention, fertilized egg reaches more than 95%, incubation rate reaches more than 92%, survival rate reaches more than 90%, and also has a large amount of trace elements in the Sepiella maindroni product that obtains at last, has improved nutrition.
Embodiment
Further specify the present invention below in conjunction with embodiment.
Embodiment
1) parent's selects and combo
According to the natural propagation method of graceful formula sepiella maindroni de Rochebrune, respectively screening health, the graceful formula sepiella maindroni de Rochebrune of sexually matured male and female are done the parent, and then the graceful formula sepiella maindroni de Rochebrune of male and female carries out combo by 1~2:1;
2) parent supports temporarily
The graceful formula sepiella maindroni de Rochebrune that combo is good is supported temporarily in the indoor culture pond, it is 20 that the pond water temperature is cultivated in control, 22 or 25 ℃, when water temperature is lower than 20 ℃, induced spawning progressively heats up, heating gradient is 0.5 ℃/day, control seawater salinity 25,28 or 30, it is 50 that pool water level is cultivated in control, 55 or 60cm, photoperiod L:D is 4:20, simultaneously graceful formula sepiella maindroni de Rochebrune is carried out 5,7 or the stimulation by running water 10 of 8m/min, 12 or 15 minutes, pressed artemia larvae during supporting temporarily in per 2 hours, vitamin, oppossum shrimp, the mineral matter feed of alternately throwing something and feeding, feeding volume is 0.3 of parent's weight, 0.4 or 0.6 times; Described mineral matter is by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI by weight for 1.5:1.0:3.0:0.6 is mixed to get, or by FeSO
4H
2O, CuSO
45H
2O, MgSO
4With KI by weight for 1:0.8:2:0.5 is mixed to get, or by FeSO
4H
2O, CuSO
45H
2O, MgSO
4Be mixed to get for 2:1.2:4:0.8 by weight with KI;
3) collect fertilized egg
Utilize the egg apparatus of cultivating in the pond to collect fertilized egg;
4) artificial incubation
Fertilized egg is positioned in the cultivation pond, it is 20,23 or 25 ℃ that the pond water temperature is cultivated in control, when water temperature is lower than 20 ℃, induced spawning progressively heats up, heating gradient is 0.5 ℃/day, control seawater salinity 25,28 or 30, and the water-soluble oxygen amount is more than or equal to 6mg/l, it is 20,30 or 40cm that pool water level is cultivated in control, and the control culture density is 1500/m
2
5) seedling culture
Begin the feed of throwing something and feeding in 6 hours behind the seedling incubation of membrane, the powder-material of front 5 days per 4 hours throw something and feed hairtail and Penaeus Vannmeis, feeding volume are 1.2,1.4 or 1.5 times of seedling weight; Per 6 hours throwing mixed feeds after 5 days, feeding volume is 1.8,2.2 or 2.5 times of seedling weight, described mixed feed is by artemia larvae: vitamin: oppossum shrimp: food starch: mineral matter: flavones is that 3:0.6:3:1.5:0.6:0.3 is mixed to get, or by artemia larvae: vitamin: oppossum shrimp: food starch: mineral matter: flavones is that 2:0.5:2:1:0.4:0.2 is mixed to get, or by artemia larvae: vitamin: oppossum shrimp: food starch: mineral matter: flavones is that 4:0.8:2~4:2:0.8:0.5 is mixed to get.Above-mentioned food starch is barley starch, wheaten starch, corn starch or potato starch.
Adopted concrete grammar of the present invention to 2000 of female graceful formula sepiella maindroni de Rochebrunes in 2011,1000 of male graceful formula sepiella maindroni de Rochebrunes are cultivated, mating, reproduction test, and last fertilized egg reaches 96%, and incubation rate reaches 94%, and survival rate reaches 92%.