CN102550885A - Feed for parent fish of Cynoglossus semilaevis - Google Patents
Feed for parent fish of Cynoglossus semilaevis Download PDFInfo
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- CN102550885A CN102550885A CN2012100651639A CN201210065163A CN102550885A CN 102550885 A CN102550885 A CN 102550885A CN 2012100651639 A CN2012100651639 A CN 2012100651639A CN 201210065163 A CN201210065163 A CN 201210065163A CN 102550885 A CN102550885 A CN 102550885A
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Abstract
The invention discloses a feed for parent fish of Cynoglossus semilaevis and belongs to a technology for preparing a marine fish compound feed. In the feed, the adding amount of fat is 8 percent, and the ratio of n-3 fatty acid to n-6 fatty acid is 2.81. The fat added into the feed for the parent fish of the Cynoglossus semilaevis is fish oil and soybean oil, and the content of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the fish oil is higher than 30 percent; and according to the formula, the feed for the parent fish of the Cynoglossus semilaevis comprises 70 percent of white fish meal, 6 percent of casein, 10 percent of flour, 2 percent of phospholipid, 1 percent of choline, 0.5 percent of vitamin C, 0.5 percent of multi-vitamin, 0.5 percent of complex mineral, 1.5 percent of calcium hydrophosphate, 2.5 percent of soybean oil and 5.5 percent of fish oil. A certain proportion of fish oil which is rich in the EPA and the DHA is added into the feed for the Cynoglossus semilaevis, and is matched with the soybean oil, so that the n-3 fatty acid and the n-6 fatty acid in an appropriate ratio are provided for the Cynoglossus semilaevis, the egg laying amount of the Cynoglossus semilaevis can be improved, the abnormality rate of fries can be reduced, and the lengths of 12-day fries can be increased.
Description
Technical field:
The invention belongs to the preparation technique of seawater fish mixed feed, relate to a kind of Cynoglossus semilaevis parent population feed particularly.
Background technology:
Along with the fast development of China's culture fishery, the importance that the aquatic livestock parent population is cultured work highlights.Have a lot of researchs to show that the parent population reproductive performance receives the influence of parent population feeding quality in recent years, it directly influences its gonad development, and smart archiblast amount and seed are good and bad, thus the development of restriction aquatic livestock seed work.As everyone knows, aquatic animal to grow institute energy requirement mainly come from fat, it provides essential fatty acid for the fish body, and it still is the carrier of liposoluble vitamin.Fat content can not cause the metabolic disorder of fish body completely in the feed; Protein utilization descends in the feed; Essential fatty acid in parent population breeding body fat in period, such as n-3 series highly unsaturated fatty acid and arachidonic acid for improving the irreplaceable effect of having grown of some seawater milt archiblast amounts and young postlarva.(Effects of high levels of n-3HUFA in broodstock diet on egg quality and egg fatty acid composition of Japanese flounder such as Furuita; Paralichthys olivaceus Aquaculture 210 (2002) 323-333) the research arachidonic acid is to the influence of lefteye flounder reproductive performance and offspring's quality; Find that the arachidonic acid of interpolation 0.6g/100g feed in the feed can improve the reproductive performance of lefteye flounder, the arachidonic acid that adds the 1.2g/100g feed in the feed has a negative impact to ovum and young quality.
Cynoglossus semilaevis (Cynoglossus semilaevis); Be commonly called as dragon profit, field sowthistle herb, be famous and precious seawater fish, the large-scale flatfish class in China Bohai Sea that mainly distributes, Huanghai Sea marine site; The Cynoglossus semilaevis fast growth; Meat flavour is delicious, and economic worth is very high, is a kind of comparatively desirable aquaculture fish (Li Sizhong etc. 1995).Because Cynoglossus semilaevis male quantity in natural marine site is few and sexual gland is undeveloped, so a little less than the self-reproduction ability, natural resources is very limited at present.In recent years, China's Cynoglossus semilaevis artificial breeding technology has also had significant progress, but also few about the research of Cynoglossus semilaevis parent population nutritional need.
Summary of the invention:
The technical problem that the present invention will solve is to propose a kind of Cynoglossus semilaevis parent population feed, can improve the reproductive performance of Cynoglossus semilaevis parent population, improves the quality of ovum and young postlarva.
The present invention accomplishes by following operating technology:
A kind of Cynoglossus semilaevis parent population feed, fatty addition is 8% in the feed, the n-3/n-6 fatty acid ratio is 2.81.
The fat that adds in the described Cynoglossus semilaevis parent population feed is fish oil and soya-bean oil, and EPA and DHA content are higher than 30% in the fish oil;
The prescription of described Cynoglossus semilaevis parent population feed: white fish meal 70%, casein 6%, flour 10%, phosphatidase 12 %, choline 1%, vitamin C 0.5%, B B-complex 0.5%, composite mineral matter 0.5%, calcium monohydrogen phosphate 1.5%; Soya-bean oil 2.5%, said fish oil 5.5%;
Described B B-complex (mg/kg or g/kg feed): thiamine, 25mg; Riboflavin, 45mg; Puridoxine hydrochloride, 20mg; Cobastab
12, 0.1mg; Vitamin K
3, 10mg; Inositol, 800mg; Pantothenic acid, 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D, 5mg; Vitamin E, 120mg; Inferior powder 18.67g;
Described composite mineral matter (mg/kg or g/kg feed): sodium fluoride, 2mg; KI, 0.8mg; Cobalt chloride, 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 3000mg; Common salt, 100mg; Zeolite powder, 15.51g.
The present invention's beneficial effect compared with prior art:
1, having added fish meal and casein in the feed is protein sources, can make feed that a rational nutrition composition is arranged, can satisfy the Cynoglossus semilaevis parent population to protein and amino acid whose nutritional need.
2, the present invention adds the fish oil that certain proportion is rich in EPA and DHA in the Cynoglossus semilaevis feed; Be used with soya-bean oil,, can improve the egg laying amount of Cynoglossus semilaevis for Cynoglossus semilaevis provides appropriate proportioning n-3/n-6 aliphatic acid; Reduce the prelarva abnormal rate, improve the length of 12 age in days prelarvas.
The specific embodiment:
Be described in detail technology contents of the present invention through embodiment below.
Present embodiment mainly is the screening to different n-3/n-6 fatty acid proportions in the Cynoglossus semilaevis parent population feed; Different with soya-bean oil proportionings nursing Cynoglossus semilaevis 8 weeks of parent population with DHA fish oil of EPA are rich in utilization, observe its laying performance and offspring's quality with proof the present invention beneficial effect compared with prior art.
Respectively with the proportioning FO (fish oil 100%) (fish oil 8%) of different fish oil and soya-bean oil; FSO1 (fish oil: soya-bean oil=7: 1, fish oil 7.0%, soya-bean oil 1.0%); FSO2 (fish oil: soya-bean oil=2.2: 1, fish oil 5.5%, soya-bean oil 2.5%); FSO3 (fish oil: soya-bean oil=1: 1, fish oil 4%, soya-bean oil 4%); FSO4 (fish oil: soya-bean oil=1: 4.3, fish oil 1.5%, soya-bean oil 6.5%) processes mixed feed as fat source, and prescription uses the composition and the content of Agilent 7890A type gas chromatograph assay determination aliphatic acid like table 1.Chromatographic column is DB-FFAP (30m * 0.32mm * 0.25 a μ m) capillary chromatographic column, analyzes and adopts temperature programming control.70 ℃ of initialization 3min, the speed with 3 ℃/min rises to 220 ℃ then, keeps 33min.The temperature of detector is set to 280 ℃, 220 ℃ of sample feeding mouth temperature.(available from Supelco, LotNo.LA-98232) retention time is confirmed sample aliphatic acid kind, and the peak area normalization method carries out quantitative assay according to standard aliphatic acid.Record that the n-3/n-6 fatty acid ratio is respectively 10.40,5.21,2.81,1.71,0.87 in FO, FSO1, FSO2, FSO3, the FSO4 feed.
Table 1 test feed is formed
B B-complex (mg/kg or g/kg feed): thiamine, 25mg; Riboflavin, 45mg; Puridoxine hydrochloride, 20mg; Cobastab
12, 0.1mg; Vitamin K
3, 10mg; Inositol, 800mg; Pantothenic acid, 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D, 5mg; Vitamin E, 120mg; Inferior powder 18.67g.
Composite mineral matter (mg/kg or g/kg feed): sodium fluoride, 2mg; KI, 0.8mg; Cobalt chloride, 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 3000mg; Common salt, 100mg; Zeolite powder, 15.51g.
Process mixed feed by above prescription and feed Cynoglossus semilaevis 8 weeks of parent population, select the female parent population of 60 tails at random, the male parent population of 20 tails is divided after weighing at random in 5 cement pits, and 12 tail rauns, 4 tail milters are arranged in each cement pit.
Test fish culture pond specification is 2m * 2m * 1.8m.Cultivate depth of water 80cm, cultivating water is the sand filtration seawater.Flowing water is cultivated, and day quantity of exchanged water is 2-5 times of the cultivation water body, and salinity: 30-33 is cultivated in continuous charge, and pH7.6-8.2, dissolved oxygen remain on more than the 6mg/L, NH4
+-N content≤0.2mg/L.With gobo and the incandescent lamp regulation and control intensity of illumination and the illumination rhythm and pace of moving things, light application time is elongated gradually by 8h-12h.The experimental session water temperature remains between 19-25 ℃.Be satiated with food every day and throw something and feed twice, continued for 8 week at 6:00 and 19:00 respectively.Each organizes relative egg laying amount, come-up ovum rate and incubation rate such as the table 2 of parent population.
Table 2 is respectively organized relative egg laying amount, come-up ovum rate and the incubation rate of parent population
Annotate: with the expression significant difference (P＜0.05) that has different letters in the data in the delegation
Can find out that from last table the highest (ratio that is n-3/n-6 aliphatic acid is 2.81 to relative egg laying amount, fish oil: soya-bean oil=2.2: 1), be significantly higher than FSO3 and FSO4 group (P＜0.05) with FSO2 group; On hatch rate with FSO2 group the highest (be fish oil: soya-bean oil=2.2: 1, the ratio of n-3/n-6 aliphatic acid are 2.81), be significantly higher than FSO3 and FSO4 group (P＜0.05), do not have significant difference (P＞0.05) with FO, FSO1 group; The highest (ratio that is n-3/n-6 aliphatic acid is 2.81 to incubation rate, fish oil: soya-bean oil=2.2: 1), be significantly higher than FSO3, FSO1, FO and FSO4 group (P＜0.05) with FSO2 group.
The different n-3/n-6 fatty acid rates of table 3 are to the influence of each group experiment fish offspring quality
Annotate: with the expression significant difference (P＜0.05) that has different letters in the data in the delegation
12 days survival rate=12 age in days prelarvas survival number/prelarva sum * 100%
Prelarva abnormal rate=lopsided prelarva number/prelarva sum * 100%
Prelarva number when N begins for experiment; Hi is the dead cumulative amount of i days prelarva, and k mantissa for survival is 0 o'clock a fate.
Abnormal rate by the visible FO of table 3, FSO1, FSO2 group prelarva is minimum, does not have significant difference between three groups, significantly is lower than FSO3, FSO4 (P＜0.05); Rate of fertilization is the highest with FSO2, FSO3 group, is significantly higher than other groups (P＜0.05); The survival activity index is the highest with the FSO2 group, is significantly higher than other groups (P＜0.05); 12 age in days prelarva total lengths are the highest with the FSO2 group, are significantly higher than other groups (P＜0.05); There was no significant difference between each group of ovum footpath.
Visible by above experiment; In Cynoglossus semilaevis parent population feed, add and be rich in different with the soya-bean oil proportionings nursing of EPA Cynoglossus semilaevis parent population with DHA fish oil; The ratio of adjustment fish oil and soya-bean oil makes that the n-3/n-6 fatty acid proportion reaches at 2.81 o'clock in the feed, can effectively improve the laying performance of Cynoglossus semilaevis parent population; And survival activity index, rate of fertilization and 12 age in days prelarva total lengths significantly raise, and the prelarva abnormal rate significantly descends.
Claims (2)
1. a Cynoglossus semilaevis parent population feed is characterized in that fatty addition is 8% in the said feed, and the n-3/n-6 fatty acid ratio is 2.81.
2. a kind of Cynoglossus semilaevis parent population feed according to claim 1 is characterized in that the fat that adds in the described Cynoglossus semilaevis parent population feed is fish oil and soya-bean oil, and EPA and DHA content are higher than 30% in the fish oil;
The prescription of described Cynoglossus semilaevis parent population feed: white fish meal 70%, casein 6%, flour 10%, phosphatidase 12 %, choline 1%, vitamin C 0.5%, B B-complex 0.5%, composite mineral matter 0.5%, calcium monohydrogen phosphate 1.5%; Soya-bean oil 2.5%, said fish oil 5.5%;
The every 1kg feed of described B B-complex: thiamine, 25mg; Riboflavin, 45mg; Puridoxine hydrochloride, 20mg; Cobastab
12, 0.1mg; Vitamin K
3, 10mg; Inositol, 800mg; Pantothenic acid, 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D, 5mg; Vitamin E, 120mg; Inferior powder 18.67g;
The every 1kg feed of described composite mineral matter: sodium fluoride, 2mg; KI, 0.8mg; Cobalt chloride, 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 3000mg; Common salt, 100mg; Zeolite powder, 15.51g.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919182A (en) * | 2012-11-21 | 2013-02-13 | 浙江海洋学院 | Propagation method of sepiella maindroni |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN104798995A (en) * | 2015-05-20 | 2015-07-29 | 中国水产科学研究院黄海水产研究所 | Saladfish young fish feed |
| CN104957432A (en) * | 2015-07-23 | 2015-10-07 | 辽东学院 | Zebrias zebra larva feed |
| CN105104856A (en) * | 2015-08-28 | 2015-12-02 | 中国水产科学研究院黄海水产研究所 | Feed capable of improving reproductive capacity and increasing fertility rate of parent fishes of cynoglossus semilaevis |
| CN105249024A (en) * | 2015-11-13 | 2016-01-20 | 辽东学院 | Zebrias zebra parent fish feed |
| CN106615984A (en) * | 2015-11-03 | 2017-05-10 | 张东 | Breeding feed for Cynoglossus semilaevis Gunther parent fishes and preparation method |
| CN107258622A (en) * | 2017-07-12 | 2017-10-20 | 中国水产科学研究院黄海水产研究所 | A kind of dietary methods of regulation and control Cynoglossus semilaevis parent population sex hormone secretion |
| CN108029902A (en) * | 2018-01-04 | 2018-05-15 | 广东越群海洋生物研究开发有限公司 | A kind of turning property of tongue sole feed and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919182A (en) * | 2012-11-21 | 2013-02-13 | 浙江海洋学院 | Propagation method of sepiella maindroni |
| CN102919182B (en) * | 2012-11-21 | 2014-10-08 | 浙江海洋学院 | Propagation method of sepiella maindroni |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN104798995A (en) * | 2015-05-20 | 2015-07-29 | 中国水产科学研究院黄海水产研究所 | Saladfish young fish feed |
| CN104957432A (en) * | 2015-07-23 | 2015-10-07 | 辽东学院 | Zebrias zebra larva feed |
| CN105104856A (en) * | 2015-08-28 | 2015-12-02 | 中国水产科学研究院黄海水产研究所 | Feed capable of improving reproductive capacity and increasing fertility rate of parent fishes of cynoglossus semilaevis |
| CN105104856B (en) * | 2015-08-28 | 2018-06-26 | 中国水产科学研究院黄海水产研究所 | A kind of feed for improving Cynoglossus semilaevis fertility of parent fish and rate of fertilization |
| CN106615984A (en) * | 2015-11-03 | 2017-05-10 | 张东 | Breeding feed for Cynoglossus semilaevis Gunther parent fishes and preparation method |
| CN105249024A (en) * | 2015-11-13 | 2016-01-20 | 辽东学院 | Zebrias zebra parent fish feed |
| CN107258622A (en) * | 2017-07-12 | 2017-10-20 | 中国水产科学研究院黄海水产研究所 | A kind of dietary methods of regulation and control Cynoglossus semilaevis parent population sex hormone secretion |
| CN108029902A (en) * | 2018-01-04 | 2018-05-15 | 广东越群海洋生物研究开发有限公司 | A kind of turning property of tongue sole feed and preparation method thereof |
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| CN102550885B (en) | 2014-03-26 |
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