CN101002547A - Method for promoting food intaking of Banhuashe Soleidae fish - Google Patents
Method for promoting food intaking of Banhuashe Soleidae fish Download PDFInfo
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- CN101002547A CN101002547A CNA2007100131421A CN200710013142A CN101002547A CN 101002547 A CN101002547 A CN 101002547A CN A2007100131421 A CNA2007100131421 A CN A2007100131421A CN 200710013142 A CN200710013142 A CN 200710013142A CN 101002547 A CN101002547 A CN 101002547A
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- feed
- cynoglossus semilaevis
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- time
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
A method for promoting the appetite of half-smooth tongue-sole includes such steps as mixing betaine and composite amino acid with bait, lighting by 0.5-30 LUX the time for feeding bait is 6:00, 18:00 and 22:00, and making the vibration frequency for feeding forage to be 0.1-5 Hz.
Description
Technical field:
The invention belongs to marine fish culture, is in the breeding production condition, promotes the method for food intaking of Banhuashe Soleidae fish.
Background technology:
Cynoglossus semilaevis (Cynoglossus semilaevis G ü nter), be commonly called as field sowthistle herb, sole order, sole rice, belong to Pleuronectiformes Pleuronectiformes, Cynoglossidae Cynoglossidae, natural distribution is coastal in China, with the Bohai Sea, the Huanghai Sea is many, is the large-scale famous and precious fish that dwell at the end.
China is in the later stage eighties 20th century, and Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science has carried out basic research (Jiang Yanwei, 1988,1993) such as Cynoglossus semilaevis age and growth, breeding habit and the ecology of laying eggs, early development.On the research basis in the past, from 2002, Huanghai Sea aquatic products research institute bore " research of Cynoglossus semilaevis genital regulating and artificial breeding technology " project, realized that the scale seed of Cynoglossus semilaevis produces (Liu Xuezhou, 2006).After the seed technological break-through, show urgent the correlative study day of cynoglossus semilaevis cultivation pattern, only make a breakthrough, the breed of this kind can be formed an industry in the technology of forming.According to the observation, propagating artificially under the condition, the feeding behaviour of Cynoglossus semilaevis is very special, be to crawl the end of based on to ingest, the feeding behaviour that this is special, it raises very difficult than other jumping up property fish that ingest, and shows as that ingestion ability is low, phenomenons such as poor growth and lethality height, and this has become the bottleneck of cynoglossus semilaevis cultivation industrialized development.How to promote ingesting of Cynoglossus semilaevis, the breed level that improves Cynoglossus semilaevis is most important, does not also have relevant report in this respect at present.
Summary of the invention:
The objective of the invention is by the taking of the short measure of ingesting of Cynoglossus semilaevis, improve and propagate ingesting of Cynoglossus semilaevis under the condition artificially, save feed, improve growth rate.
The present invention makes by following technical scheme: its method comprises the determining of time and the determining of the vibration frequency of the feed of throwing something and feeding of the feed of determining, throw something and feed of the determining of bait, intensity of illumination.
1, add bait in feed, adding bait is betain and compound amino acid;
2, the intensity of illumination is 0.5-30LUX;
3, throw something and feed time of feed is 6:00,18:00,22:00;
4, providing the vibration frequency of feed is 0.1-5Hz.
The present invention and prior art contrast are characterized in:
Culture Cynoglossus semilaevis and promote the method for ingesting by adopting, 1, improved the food intaking of Banhuashe Soleidae fish rate, the grazing rate behind the feed of throwing something and feeding in 10 minutes is more than 95%, compares with common cultural method, the grazing rate of common breeding feed only is 50%; 2, saved manufactured feed, improved growth rate, growth rate can improve more than 20%; 3, this method is simple, cost is low, easy to use.
Embodiment:
Below by embodiment the technology of the present invention method is described in detail:
This research has carried out promoting the research of the method for ingesting on Laizhou, Shandong and other places, and this method is applied to the large-scale cultivation experiment, and detailed process is as follows:
1, promote the method ingest:
This method comprises the determining of time and the determining of the vibration frequency of the feed of throwing something and feeding of the feed of determining, throw something and feed of the determining of bait, intensity of illumination.
(1), determining of bait:
In the Cynoglossus semilaevis experimental tank, add different baits, first set reaction time and the duration of the reaction of gamma camera record Cynoglossus semilaevis to various baits is set.
Cynoglossus semilaevis sees Table 1 to the experimental result of different bait reactions
Table 1 Cynoglossus semilaevis is to the reaction of different baits
| Bait | The first set reaction time (s) | Duration of the reaction (min) | The extent of reaction |
| L-glycine Pidolidone betaine 1B Taurine L-arginine beet alkali hydrochlorate compound amino acid L-hydroxyproline | - (52.67±6.74) c(10.33±3.18) ab- (18.33±4.41) ab(24.00±3.51) b(53.33±11.67) c(1.00±0.00) a- | - 4-5 3-4 - 1-2 2-3 0-1 5-6 - | Reactionless strong reactionless weak by force reactionless |
Above-mentioned result of the test shows: there is notable difference in Cynoglossus semilaevis to first set reaction time, duration and the extent of reaction of various different baits.In 9 kinds of used in this experiment baits, the shortest to the first set reaction time of compound amino acid, be (1.00 ± 0.00) s, the duration is the longest, is 5-6min, and the extent of reaction is strong.At single bait, the most responsive to betain, its first set reaction time be (10.33 ± 3.18) s, and the first set reaction time of comparison 18-MAA is slightly long, but the two comparing difference not remarkable (P＞0.05), the extent of reaction is also very strong; The first set reaction time to beet alkali hydrochlorate is the longest, can reach (53.33 ± 11.67) s, and the duration is the shortest, only is 0-1min, and still, the extent of reaction is still strong; The first set reaction time to L-glutamic acid is long, be (52.67 ± 6.74) s, and the duration is also longer, is 4-5min; To the then not reaction of L-glycine, L-lysine and L-hydroxyproline.Test shows that compound amino acid and betain are best to the food calling effect of Cynoglossus semilaevis.
(2) determining of intensity of illumination:
Respectively with cynoglossus semilaevis cultivation under the above illumination condition of 500LUX and under the following illumination condition of 30LUX after 30 days, carry out retinal tissue and learn research.Test material is got eye, and is fixing after pricking several apertures on the cornea.Adopt fixedly 24h of Holland-BouinShi fixer, ethanol dehydration, after dimethylbenzene is transparent, material according to horizontal or tangential section, is required to be embedded in the paraffin, do continuously-directional section (thick 5 μ m), H.E dyeing, the NIKON microscopically is observed, and the result shows that its melanin of Cynoglossus semilaevis under the above illumination condition of 500LUX is distributed in around the outer section of cone layer densely; And the following half-light of 30LUX shines under the condition, and Cynoglossus semilaevis melanin is evenly distributed, and studies show that Cynoglossus semilaevis is adapted to the environment of low light according to intensity.
Be verified by behaviouristics again: adopt that initial average body is long to be the Cynoglossus semilaevis of 20cm, culture under different illumination intensity, through the growth fraction of indexs such as 60 days its grazing rates and growth rate, result of the test sees the following form 2:
The growth of ingesting under the table 2 Cynoglossus semilaevis different illumination conditions
| Intensity of illumination (LUX) | Grazing rate (%) | Average growth rate (%) |
| 0.5-30 50-100 100-500 | 100 60 50 | 8.9 6.7 4.3 |
Show that by histology and ethological result of the test the Cynoglossus semilaevis growth best intensity of illumination of ingesting is 0.5-30LUX.
(3) throw something and feed the determining of time of feed:
Dwell at the end Cynoglossus semilaevis of life of battalion is determined to see Table its rhythm and pace of moving things of ingesting 3, and formulate the best thus and throw something and feed the time by the food ration of measuring its different time sections:
Table 3 Cynoglossus semilaevis is in the food ration of different time
| Time | 3:00 | 6:00 | 9:00 | 12:00 | 15:00 | 18:00 | 21:00 | 24:00 |
| The ratio of the percentage of liveweight of ingesting (%) | 0.8 | 0.6 | 0.1 | 0 | 0.3 | 1.5 | 1.0 | 1.2 |
From ingest time, the best ingest time ordering of Cynoglossus semilaevis is for being 18:00,24:00,21:00,3:00,6:00,15:00.
In conjunction with the operability of breeding production, the feeding time of throwing something and feeding of Cynoglossus semilaevis is decided to be: 6:00,18:00,22:00.
(4) throw something and feed the determining of vibration frequency of bait:
The relatively reaction of ingesting to different vibration frequencies simulation feeds of Cynoglossus semilaevis, the feed vibration frequency of eating with the happiness of determining Cynoglossus semilaevis.Vibration frequency drives generation by the self-control electric device.Experimental result shows: vibration frequency is 0 o'clock, and grazing rate is 14%.When vibration frequency was 0.1-5Hz, grazing rate was 70-93%; And when vibration frequency during greater than 5Hz, Cynoglossus semilaevis does not have initiatively predation, even flight behavior occurs, tests Cynoglossus semilaevis happiness food low-frequency vibration feed as can be seen, and suitable vibration frequency is 0.1-5Hz.
2, the short large-scale cultivation test of ingesting of Cynoglossus semilaevis
(1) cultural method: 1. in feed, add bait: in feed, add betain and compound amino acid; 2. the intensity 0.5-30LUX of illumination; 3. throw something and feed time of feed 6:00; 18:0022:00; The vibration frequency that 4. feed is provided is 0.1-5Hz.
(2) carry out the breed Cynoglossus semilaevis test of scale in Shandong Mingbo Aquatic Product Co., Ltd., Laizhou, test mantissa 3000 tails, the long 17cm breed of average body; Adopt above method to culture, the average grazing rate of throwing something and feeding is 96%, and through 9 months breed, average body reached 45cm, the average body 650g that weighs, and growth rate is higher than common breed level 20%.
Claims (1)
1, a kind of method that promotes food intaking of Banhuashe Soleidae fish, its method comprise the determining of time and the determining of the vibration frequency of the feed of throwing something and feeding of the feed of determining, throw something and feed of the determining of bait, intensity of illumination, it is characterized in that:
1) bait that, adds in feed is: betain and compound amino acid;
2), the intensity of illumination is: 0.5-30LUX;
3), throw something and feed time of feed: 6:00,18:00,22:00;
4), provide the vibration frequency of feed to be: 0.1-5Hz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100131421A CN101002547A (en) | 2007-01-09 | 2007-01-09 | Method for promoting food intaking of Banhuashe Soleidae fish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100131421A CN101002547A (en) | 2007-01-09 | 2007-01-09 | Method for promoting food intaking of Banhuashe Soleidae fish |
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| CN101002547A true CN101002547A (en) | 2007-07-25 |
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| CNA2007100131421A Pending CN101002547A (en) | 2007-01-09 | 2007-01-09 | Method for promoting food intaking of Banhuashe Soleidae fish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550885A (en) * | 2012-03-13 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Feed for parent fish of Cynoglossus semilaevis |
-
2007
- 2007-01-09 CN CNA2007100131421A patent/CN101002547A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550885A (en) * | 2012-03-13 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Feed for parent fish of Cynoglossus semilaevis |
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