CN107348250A - A kind of hirudinaria manillensis feed and preparation method thereof - Google Patents
A kind of hirudinaria manillensis feed and preparation method thereof Download PDFInfo
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- CN107348250A CN107348250A CN201710779146.4A CN201710779146A CN107348250A CN 107348250 A CN107348250 A CN 107348250A CN 201710779146 A CN201710779146 A CN 201710779146A CN 107348250 A CN107348250 A CN 107348250A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The present invention relates to a kind of hirudinaria manillensis feed and preparation method thereof, category hirudiniculture technique for producing feed field.The present invention obtains thick hirudinaria manillensis finished feed after the raw materials such as globulin powder, SDPP, egg, vitamin C, B B-complex, honey, sodium chloride are stirred according to certain percentage by weight and water, pours into the animal intestine cleaned up thick hirudinaria manillensis finished feed when feeding and is fed.Preparation method of the present invention is simple, and cost is cheap, meets the nutritional requirement of hirudinaria manillensis growth stage, improves the survival rate of hirudinaria manillensis cultivation and shortens the culture-cycle of hirudinaria manillensis, is cultivated especially suitable for hirudinaria manillensis large-scale industrialization.
Description
Technical field
The present invention relates to a kind of hirudinaria manillensis feed and preparation method thereof, category hirudiniculture technique for producing feed field.
Background technology
The endocrine anticoagulative substance of hirudinaria manillensis body is hirudin, has preventing and treating arteriosclerosis, hypertension, high blood
The effect of diseases such as fat, thrombus and headstroke, nowadays using more and more extensive on clinical medicine.Meanwhile hirudin is in beauty
The field such as skin care item and health products also has broad application prospects.In recent years, in order to protect wild leech resource and guarantee
Supply of the hirudinaria manillensis as pharmaceutical raw material, the cause of domestic artificial feeding leech are just surging forward.Wild Japanese cures leech with the frog
The new blood of class and mammal is eats, artificial feeding hirudinaria manillensis, on the one hand because blood sources are different so that blood supplies
The promptness answered, safety and reliability cannot ensure, cause hirudinaria manillensis survival rate low and slow-growing.On the other hand,
Hirudinaria manillensis is fed using new blood, the risk for polluting breeding water body in water after remaining new blood corruption, serious shadow be present
Ring the large-scale industrialized development of artificial farming hirudinaria manillensis.Therefore, being highly desirable research and development one kind can allocate in time, and nutrition is rich
Richness, palatability is good, and sanitary safe, feeding is reliable, not polluted-water, improves the hirudinaria manillensis speed of growth and survival rate, is fabricated to
The feed of this low artificial feeding hirudinaria manillensis.
The content of the invention
It is an object of the invention to:There is provided one kind can allocate in time, and nutritious, comprehensive, palatability is good, sanitary safe,
Feeding is reliable, not polluted-water, and to improve the leech speed of growth and survival rate, and preparation method is simple and convenient, and then solves existing
There is feeding new blood to be difficult to ensure that the promptness of blood supply, safety and reliability, cause leech slow-growing and survive
Rate is low, and hirudinaria manillensis feed in water after remaining new blood corruption the problems such as pollution breeding water body and preparation method thereof.
The technical scheme is that:
A kind of hirudinaria manillensis feed, it is characterised in that it is made up of the raw material of following weight percentage:
The SDPP 1~4% of globulin powder 2~6%
The sodium chloride 0.4%~0.65% of egg 20~50%
The B B-complex 0.01%~0.1% of vitamin C 0.05%~0.1%
The water surplus of honey 1%~3%.
The preparation method of above-mentioned hirudinaria manillensis feed comprises the following steps:
1), weigh according to above-mentioned percentage by weight globulin powder respectively, be SDPP, egg, sodium chloride, vitamin C, multiple
Close vitamin and honey;
2), will the sodium chloride that weighed add excess water in stir after be configured to sodium chloride solution;
3), egg is shelled after add sodium chloride solution in, then vitamin C, B B-complex and honey are added to successively
In sodium chloride solution, 15-20 minutes obtained mixed liquor is mixed;
4), sequentially add globulin powder and SDPP in mixed liquor, and mix 0.5-2 hours again to mixing
Uniformly, thick hirudinaria manillensis finished feed is produced;Thick hirudinaria manillensis finished feed is poured into cleaning when feeding to do
Fed in net animal intestine.
A kind of hirudinaria manillensis feed, wherein the percentage by weight of each raw material is:
The SDPP 2~4% of globulin powder 4~6%
The sodium chloride 0.5%~0.6% of egg 30~50%
The B B-complex 0.05%~0.08% of vitamin C 0.06%~0.08%
The water surplus of honey 2%~3%.
A kind of hirudinaria manillensis feed, wherein the percentage by weight of each raw material is:
The SDPP 3% of globulin powder 5%
The sodium chloride 0.6% of egg 40%
The B B-complex 0.07% of vitamin C 0.07%
The water surplus of honey 3%.
Using one kind in pig blood, ox blood, sheep blood, chicken blood new blood or any ratio when described globulin powder is made by oneself
Example it is several, isolate blood cell through supercentrifuge, by blood cell after spray drying obtained powdered finished product.The present invention's
Hyperglobulinemia powder raw material uses Pu Run bio tech ltd of Weifang City product.
Using one kind in pig blood, ox blood, sheep blood, chicken blood new blood or any ratio when described SDPP is made by oneself
Several be well mixed with anti-coagulants sodium citrate of example is cooled to 3-7 DEG C, and the mixture after cooling is separated through supercentrifuge
Go out blood plasma, powdered finished product is finally made in blood plasma after spray drying.The plasma protein powder raw material of the present invention uses Weifang
Pu Run bio tech ltd of city product.
Described B B-complex is commercially available prod, wherein containing in every kilogram of B B-complex:
The raw IU of D3 320000 of the IU of vitamin A 1100000 dimensions
The mg of nicotinic acid 7800mg vitamin Es 2500
The mg biotins 8mg of vitamin B1 1000
The mg of 2000 mg vitamin B6s of vitamin B2 1000
The mg of folic acid 400mg vitamin B12s 125
The mg enzymes 1000mg of vitamin C 18000
Potassium 11g salt 45g
The mg water surplus of methionine 400.
The function of each component is as follows in hirudinaria manillensis feed of the present invention:
Globulin powder:One is high digestibility, China national feed Industry Technology Center by a large number of experiments show that:Spraying is dry
The dry external Pepsin digestibility of globulin powder is also splendid in the digested rate of various animal bodies up to more than 99%.It is the second is thin
After birth percentage of damage is high, and fresh animal blood separates through supercentrifuge, and blood cell film is crushed in separation process by centrifugal force,
Hemoglobin and Dissolve things inside are discharged, drastically increases the water solubility, digestibility and absorptivity of globulin powder.It is the third is raw
Thing high safety, more than 250 DEG C flash-sterilizations, the inactivation of harmful bacteria and virus is ensure that, it is safe and reliable.It four
It is containing abundant high-quality Organic Iron, rich in the organic PORPHYRIN IRONs of 2500ppm, is easily absorbed by cub and hirudinaria manillensis.Through country
Authoritative testing agency's sampling measure display, globulin powder gross protein value are up to more than 88%, wherein lysine and valine
7.4% and 7.5% is up to respectively, and growth and breeding of the abundant essential amino acid to hirudinaria manillensis have very big facilitation.
SDPP:It is to report and study a kind of more novel protein resource in feedstuff industry in recent years.Will be fresh
Animal blood is collected in the cold-storage jar added with anti-coagulants sodium citrate, and anticoagulation is through the blood cell that is centrifuged at a high speed out, blood plasma portion
Divide and heated 25 minutes under the conditions of 32 DEG C, be spray-dried at 207 DEG C 1~2 minute, be then dehydrated 1~2 minute at 93 DEG C,
Obtain the finished product of fine powder.SDPP had both eliminated bloody stink made of this technique, protected blood plasma again
The activity of globulin, improve the digestive utilization ratio of protein.Research shows that SDPP is in early weaning pigling daily ration
High-quality protein source, can be higher than skimmed milk power as skimmed milk power and the substitute of dry whey, its palatability.Feeding is sucked blood water
Leech can improve its speed of growth.
Sodium chloride:Prepared by the concentration of physiological saline, supply dielectric and maintenance body fluid tension force, improve the bait of hirudinaria manillensis
Expect palatability.There is sale in biochemical reagents company to chlorination sodium raw materials at home.
Vitamin C:Promote the phagocytosis of leucocyte and the formation of reticuloendothelial system and antibody, strengthen hirudinaria manillensis pair
It is various stress phenomenon adaptability, improve the immunity and premunition of hirudinaria manillensis, maintain and seek necessary to hirudinaria manillensis breeding
Support material.
B B-complex:It is the composite dosage form vitamin that various vitamins synthesize according to doses ratio.Prevention is sucked blood
The vitamin-deficiency that leech is triggered by dietary unbalance.
Honey:Carbon source is provided, improves hirudinaria manillensis functions of intestines and stomach and improves its immunity, regulation hirudinaria manillensis cultivation water ring
Border, improve the palatability of hirudinaria manillensis feed.
Egg:Containing substantial amounts of vitamin and mineral, and the protein with high biological value.Containing about egg in albumen
White matter 12%, mainly ovalbumin, also containing the thing such as a certain amount of riboflavin, niacin, biotin and calcium, phosphorus, iron in albumen
Matter.The main comprise material of yolk is vitellin, and fat content is 28.2% in addition, the fat ovum belonged in phospholipid more
Phosphatide.Yolk contains abundant vitamin A and vitamin D, and contains the mineral matters such as higher iron, phosphorus, sulphur and calcium.
In order to illustrate effect of the present invention to raising hirudinaria manillensis survival rate, present invention selection Hirudo japonica is subjects
Feeding experiment is carried out, result of the test is as follows:
(1)Experimental design
Choose 100 urosomes and focus on that 0.4-0.7 grams of size is homogeneous, vigor is good and the seamless leech in surface, and be randomly divided into experiment
Group and each 50 tail of control group, are numbered with numbering, after wiping its body surface moisture content and foul with the gauze of moistening, weigh successively and record number
According to being respectively placed in long 60cm, wide 40cm, high 26cm 80 mesh test group net cages and control group net cage, and networking will be tested
Case and control group net cage are respectively placed in the plastic box of equal size, add water 8000ml toward plastic box is interior;
Test group and two groups of leech body weight, physical developmental states of control group are basically identical, no significant difference, have comparativity.
(2)Test method
Test group leech feeds feed of the present invention, and feed of the present invention is poured into the pig cleaned up
In intestines, the feeding of 9 points of every morning, feed weekly once, the bowel lavage forage volume of the present invention of feeding is the test group that is fed
5 times of leech gross weight, feed 7 months;
Control group leech feeds fresh pig blood bait, and fresh pig blood is poured into the chitling cleaned up
In, the feeding of 9 points of every morning, feed weekly once, the fresh pig blood abundance of food of feeding is the control group leech that is fed
5 times of gross weight, feed 7 months;
(3)Result of the test
Initial average weight:Total mantissa of each leech body weight sum/leech
Last average weight eventually:Total mantissa of each leech body weight sum/leech
Average weight growth rate:(Last average weight-initial average weight eventually)/ initial average weight:
Survival rate:Last leech live body quantity/initial dispensing leech live body quantity eventually
The comparative test result of table one
In summary, hirudinaria manillensis feed of the present invention and preparation method thereof, using globulin powder, SDPP, egg, chlorine
Change the raw materials such as sodium, vitamin C, B B-complex, honey, be prepared by 4 steps of the invention.It is safe and reliable, fit
Mouth property is good, and cost is low, also the bait of alternative batrachia, mammal new blood as hirudinaria manillensis, drastically increases suction
The survival rate of watery blood leech, the industrialization large-scale farming of hirudinaria manillensis is advantageous to, is caused after avoiding new blood corruption
Hirudinaria manillensis cultivates the pollution of water ecological environment, and the promptness of new blood supply, safety and reliability are difficult to ensure that
The problem of, while avoid the slow-growing risk low with survival rate of leech triggered due to the defects of prior art.
The beneficial effect of the present invention compared with prior art is:
The hirudinaria manillensis feed and preparation method thereof, using globulin powder, SDPP, egg, sodium chloride, vitamin C,
The all raw materials of B B-complex, honey, it is prepared by 4 steps of the invention.Substitute what is commonly used in traditional hirudinaria manillensis cultivation
New blood, avoid the occurrence of cause hirudinaria manillensis indigestion after feeding new blood such as ox blood, pig blood or because of courses of infection and
Trigger the illnesss such as haematemesis, polypide deformation, swelling, safe and reliable, palatability is good, and cost is low, and also alternative batrachia, lactation is moved
Bait of the thing new blood as hirudinaria manillensis, the speed of growth and survival rate of hirudinaria manillensis are drastically increased, it is highly beneficial
In the extensive seed breeding of the industrialization of hirudinaria manillensis, while avoid feeding new blood corruption easily occurs and cause hirudinaria manillensis
Cultivation water ecological environmental pollution is deteriorated, and bait utilization is low, wastes serious, the problem of increasing aquaculture cost.By add egg,
Vitamin C and B B-complex, compensate for because globulin powder made of spray drying and the nutrition that SDPP loses into
Point, and feed of the present invention is easier to hirudinaria manillensis and assimilate;Honey is added, improves palatability, hirudinaria manillensis feed
It is more, effectively increase the speed of growth.Solves prior art feeding new blood, it is difficult to ensure promptness, the peace of blood supply
Full property and reliability, cause slow-growing low with survival rate, and exist in water and pollute breeding water body after remaining new blood corruption
The problem of risk.
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but does not therefore limit the present invention to described
Scope of embodiments among;
The embodiment raw material components table of table two
It is first according to above-mentioned percentage by weight raw material and weighs globulin powder, SDPP, egg, sodium chloride, dimension life respectively
Plain C, B B-complex, honey;Then the sodium chloride that will be weighed, excess water is added to be configured to sodium chloride solution after stirring;
Further egg is shelled and squeezed into prepared sodium chloride solution, then successively by vitamin C, B B-complex and honey
It is added in sodium chloride solution, and mixes 15-20 minutes to well mixed obtained mixed liquor;Further in mixed liquor
In sequentially add globulin powder and SDPP, and mix 0.5-2 hours again to well mixed, that is, mixing be made
Liquid;Globulin powder and SDPP are finally sequentially added in mixed liquor, and mixes 0.5-2 hours again to mixing
Uniformly, obtain, thick hirudinaria manillensis finished feed;Thick hirudinaria manillensis finished feed is poured into when feeding clean up it is dynamic
Thing enteral, animal intestine both ends are tightened into sealing with fine rule and puts into the artificial farming casing of hirudinaria manillensis and is fed, are sucked blood
The feeding volume of leech finished feed is 5 times of hirudinaria manillensis total weight in box for breeding body, is fed weekly once.
Claims (7)
1. a kind of hirudinaria manillensis feed, it is characterised in that it is made up of the raw material of following weight percentage:
The SDPP 1~4% of globulin powder 2~6%
The sodium chloride 0.4%~0.65% of egg 20~50%
The B B-complex 0.01%~0.1% of vitamin C 0.05%~0.1%
The water surplus of honey 1%~3%.
2. a kind of hirudinaria manillensis feed according to claim 1, it is characterised in that the percentage by weight of wherein each raw material is:
The SDPP 2~4% of globulin powder 4~6%
The sodium chloride 0.5%~0.6% of egg 30~50%
The B B-complex 0.05%~0.08% of vitamin C 0.06%~0.08%
The water surplus of honey 2%~3%.
3. a kind of hirudinaria manillensis feed according to claim 1, it is characterised in that the percentage by weight of wherein each raw material is:
The SDPP 3% of globulin powder 5%
The sodium chloride 0.6% of egg 40%
The B B-complex 0.07% of vitamin C 0.07%
The water surplus of honey 3%.
4. a kind of hirudinaria manillensis feed according to claim 1, it is characterised in that described globulin powder uses pig
Blood, ox blood, sheep blood, one kind in chicken blood new blood or arbitrary proportion it is several, blood cell is isolated through supercentrifuge, by blood
Ball warp crosses obtained powdered finished product after spray drying.
5. a kind of hirudinaria manillensis feed according to claim 1, it is characterised in that described SDPP uses pig
Blood, ox blood, sheep blood, one kind in chicken blood new blood or the several of arbitrary proportion are well mixed cooling with anti-coagulants sodium citrate
To 3-7 DEG C, the mixture after cooling is isolated into blood plasma through supercentrifuge, powder is finally made in blood plasma after spray drying
Last shape finished product.
6. a kind of hirudinaria manillensis feed according to claim 1, it is characterised in that described B B-complex is commercially available production
Product, wherein containing in every kilogram of B B-complex:
The raw IU of D3 320000 of the IU of vitamin A 1100000 dimensions
The mg of nicotinic acid 7800mg vitamin Es 2500
The mg biotins 8mg of vitamin B1 1000
The mg of 2000 mg vitamin B6s of vitamin B2 1000
The mg of folic acid 400mg vitamin B12s 125
The mg enzymes 1000mg of vitamin C 18000
Potassium 11g salt 45g
The mg water surplus of methionine 400.
7. a kind of a kind of hirudinaria manillensis feed producing method for described in claim 1, it is characterised in that it comprises the following steps:
1), weigh according to above-mentioned percentage by weight globulin powder respectively, be SDPP, egg, sodium chloride, vitamin C, multiple
Close vitamin and honey;
2), will the sodium chloride that weighed add excess water in stir after be configured to sodium chloride solution;
3), egg is shelled after add sodium chloride solution in, then vitamin C, B B-complex and honey are added to successively
In sodium chloride solution, 15-20 minutes obtained mixed liquor is mixed;
4), sequentially add globulin powder and SDPP in mixed liquor, and mix 0.5-2 hours again to mixing
Uniformly, thick hirudinaria manillensis finished feed is produced;Thick hirudinaria manillensis finished feed is poured into cleaning when feeding to do
Fed in net animal intestine.
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Cited By (2)
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CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
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CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
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Inventor after: Zhang Jian Inventor after: Yu Xiang Inventor after: Gong Yuan Inventor before: Gong Yuan |
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Application publication date: 20171117 |