CN111587967A - Poecilobdella manillensis breeding feed - Google Patents
Poecilobdella manillensis breeding feed Download PDFInfo
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- CN111587967A CN111587967A CN202010551542.3A CN202010551542A CN111587967A CN 111587967 A CN111587967 A CN 111587967A CN 202010551542 A CN202010551542 A CN 202010551542A CN 111587967 A CN111587967 A CN 111587967A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/24—Animal feeding-stuffs from material of animal origin from blood
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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Abstract
The invention belongs to the technical field of aquaculture, and particularly relates to a feed for breeding Poecilobdella manillensis. The feed for feeding poecilobdella manillensis is prepared from the following raw materials in parts by weightThe material composition is as follows: 60-80 parts of animal blood freeze-dried powder, 5-10 parts of egg white, 0.1-0.8 part of calcium hydrophosphate, 0.1-0.8 part of ferrous ion compound, 0.2-0.8 part of sodium chloride, trans-4, 5—0.2-0.5 part of epoxy- (E) -2-decenal, 1-10 parts of amino acid and 1-2 parts of vitamin. The invention can improve the immunity and disease resistance of the poecilobdella manillensis, has better effects on the growth, development, reproduction and disease resistance of the poecilobdella manillensis, and has extremely high economic benefit when the poecilobdella manillensis is fed by the poecilobdella manillensis feed.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a feed for breeding Poecilobdella manillensis.
Technical Field
Poecilobdella manillensis lessson is commonly called leech with gilt edge, belongs to Annelida of Annelida, Hirudinea of Bdellida in Classification, and is not belonging to the order Articoidales (A rhynchobdellida Blanchard), medical leech (Hirudidine Whitman) and Poecilobdella manillensis, and is a special medicinal aquatic animal with high medicinal value. Comprising: break blood and dredge channels, dispel stasis and eliminate disease. Can be used for treating amenorrhea due to blood stasis, abdominal mass, apoplexy, hemiplegia, and traumatic injury. The leech has more than 2000 years of clinical application history, and is recorded in medical books and so on from Shen nong's herbal Jing, which means that the leech mainly dispels blood stasis, closes the moon and breaks blood, eliminates and accumulates, and the decoction of leech compatibility is recorded in Zhang Zhongjing Shang Han Lun and jin Kui Yao L ü e, and the Dahuang Pi Chong Wan is called a Shenfang which can lead to death. The doctor of the later generation likes this medicine: blood stasis without damaging new blood, pure water essence is generated, which has no damage to qi system, and blood stasis is still inherent in invisible genuine herbs.
Modern medical research shows that: the fresh Hirudo contains hirudin (hirudin) which is low molecular polypeptide consisting of 65-66 amino acids and is known as the most effective natural anticoagulant, and the Hirudo of the family Poecilobdellidae of the genus Poecilobdellidae contains hirudin and hirudin. The Chinese pharmacopoeia stipulates that: the antithrombin activity of leeches per gram is 16U, and the antithrombin activity of poecilobdella manillensis per gram can reach 5000U at most. It has better effect than heparin, and has anticoagulant, thrombolytic, and platelet aggregation inhibiting effects. Has strong dissolving activity, can activate dissolving system, dissolve thrombi and inhibit thrombosis formation, can resist coronary artery spasm and treat myocardial ischemia, and can eliminate arteriosclerosis plaque, reduce cholesterol and triglyceride, dilate capillary vessel, and improve microcirculation. In addition, it also has effects of protecting cranial nerve, resisting fibrosis, terminating pregnancy, inhibiting tumor cell and inhibiting tumor angiogenesis. The compound preparation has great clinical treatment advantages and wide application prospects in aspects of septicemia shock, atherosclerosis, cerebral infarction, cardiovascular diseases, hypertension, ophthalmology and other diseases with thrombus and microcirculation disturbance.
Along with the increase of market demand for Poecilobdella manillensis resources, wild Poecilobdella manillensis resources are increasingly exhausted, and the artificial breeding of Poecilobdella manillensis becomes a solution for continuously ensuring the supply of raw materials. In artificial breeding, the used feed is fresh or freeze-dried blood of animals such as pigs, cattle and sheep, and has the defects of easy pollution, easy decay, unstable quality, difficult transportation and storage and the like. Especially, the whole detection of pathogenic bacteria and viruses of zoonosis in the quarantine process can not be realized. Fresh blood is not sterilized, so that the potential safety hazard exists. Therefore, the development of the sterile, safe, nutritional and healthy poecilobdella manillensis feed becomes a key for developing industrialized breeding of the poecilobdella manillensis.
Chinese patent application No. 201610474256.5 discloses an environment-friendly growth-promoting feed for leech feeding and a preparation method thereof, wherein the feed comprises the following components in parts by weight: bean pulp: 40-50 parts of sorghum flour: 20-30 parts of shell powder: 20-30 parts of dried blood: 20-30 parts of bran: 5-10 parts of calcium hydrophosphate: 1-5 parts, calcium lactate: 1-5 parts of compound vitamin: 1-5 parts of edible oil: 1-5 parts of a growth promoter: 1-5 parts, taurine: 0.1-0.5 part of water and a proper amount of water. The feed is rich in nutrient components and comprehensive in nutrient elements, can effectively supplement nutrient components required by the growth of the leeches, effectively promote the growth, shorten the growth period of the leeches, reduce the culture cost and improve the economic benefit.
Chinese patent application No. 201810838147.6 discloses an ecological breeding feed suitable for leeches, which comprises the following components in percentage by weight: 30-50% of rice washing water, 15-30% of rice flour, 8-10% of starch, 10-15% of animal blood, 7-10% of earthworm powder, 3-8% of fish meal, 1-3% of dietary fiber, 1-3% of bone meal, 3-5% of egg yolk, 1-2% of traditional Chinese medicine juice and 1-2% of inorganic salt; the sum of the components of the formula is 100 percent. The preparation method of the ecological breeding feed for leeches prepared by adopting the formula comprises the following steps: raw material preparation → feed preparation → slurry suspension → packaging, and warehousing and refrigeration. The granular feed formula adopted in the invention comprises the following components: the feed is prepared into pasty feed, so that the feed is more beneficial to being melted in water when being fed, the absorption of the leech is improved, and the disease of the leech in the breeding process is effectively prevented by the traditional Chinese medicine juice. Further improves the taste of the feed, effectively improves the output of the fed leeches and promotes the growth of leech culture.
Chinese patent application No. 201610474179.3 discloses a disease-preventing immunity-improving coated feed for leech feeding and a preparation method thereof, which comprises core fertilizer particles and a coating layer, wherein the core fertilizer comprises the following components in parts by weight: bean pulp: 40-50 parts of sorghum flour: 20-30 parts of shell powder: 20-30 parts of dried blood: 20-30 parts of bran: 5-10 parts of calcium hydrophosphate: 1-5 parts, calcium lactate: 1-5 parts of compound vitamin: 1-5 parts of edible oil: 1-5 parts of a growth promoter: 1-5 parts, taurine: 0.1-0.5 part; the coating layer comprises the following components in parts by weight: and (3) chitosan: 10-20 parts of rheum officinale: 5-10 parts of phellodendron bark: 3-6 parts of radix scutellariae: 2-4 parts of sorbitol: 0.5-1 part of binder: 1-5 parts of edible gelatin: 5-10 parts of deionized water: 1-5 parts of acetic acid: 0.5-1 part. The feed is rich in nutrition, can promote and accelerate the development of the leech and shorten the growth period while improving the disease prevention and resistance of the leech, has a coated structure, is uniform in nutrient release rhythm and long in duration, saves the feeding cost and improves the economic benefit.
Disclosure of Invention
The invention aims to provide a safe and nutritional feed for breeding Poecilobdella manillensis, which avoids potential safety hazards of common disease transmission of people and livestock.
The feed for feeding poecilobdella manillensis provided by the invention comprises the following raw materials in parts by weight: 60-80 parts of animal blood freeze-dried powder, 5-10 parts of egg white, 0.1-0.8 part of calcium hydrophosphate, 0.1-0.8 part of ferrous ion compound, 0.2-0.8 part of sodium chloride, trans-4, 5—0.2-0.5 part of epoxy- (E) -2-decenal, 1-10 parts of amino acid and 1-2 parts of vitamin.
The animal blood freeze-dried powder is characterized in that: the blood freeze-dried powder is prepared from fresh animal blood such as pig blood, cattle blood, sheep blood, chicken blood, duck blood, goose blood and the like by a freeze-drying process, and ensures that the nutrition of the blood is not damaged. Meanwhile, by sterilization treatment, the propagation of pathogenic bacteria and viruses is avoided, and the safety of the feed is ensured.
The ferrous ion compoundIs Fe2+In particular FeS04 and FeCl2、FeCO3、Fe(C3H5O3)2And the like.
The amino acid is one or more of glutamic acid, arginine and lysine.
The vitamin is VitB1、VitB2、VitB6One or more of Vitc and VitE.
The formula of the invention contains Fe2+Trans-4, 5-epoxy- (E) -2-decenal can improve food calling and feed utilization rate.
The amino acid, the vitamin, the calcium hydrophosphate and the egg white in the formula can improve the immunity and the reproductive capacity of the poecilobdella manillensis.
The test of the Poecilobdella manillensis breeding feed on the breeding survival rate and the weight gain rate.
Materials: 60 middle seedlings of poecilobdella manillensis are obtained: yunan Poecilobdella manillensis agricultural science and technology culture company base.
Poecilobdella manillensis feed: example 1, example 2, source: self-matching.
Fresh pig blood, source: yunnan Shennong meat industry food Co.
Thrombin (140625-: china institute for testing food and drug.
Bovine fibrinogen (bovine blood) (140607 and 201841), source: china institute for testing food and drug.
The method comprises the following steps: the method comprises the steps of averagely dividing poecilobdella manillensis into 2 groups, weighing 30 poecilobdella manillensis per group, feeding the feeds and fresh pig blood (a control group) in the embodiments 1 and 2 respectively, feeding the feeds once every 7 days, counting survival rate after 30 days, weighing living bodies, freeze-drying the living bodies, crushing dried products, screening the crushed products by a No. 3 sieve, carrying out antithrombin activity detection according to a method of content determination items in quality standards of poecilobdella manillensis freeze-dried powder (YunyYPBZ-0199 plus 2013), and evaluating the feeding effect of the feed formula disclosed by the patent application by comparing the survival rate, the weight growth condition and the antithrombin activity of the poecilobdella manillensis fed by. The results are as follows:
TABLE 1 Poecilobdella manillensis breeding survival rate test results
TABLE 2 Poecilobdella manillensis breeding weight gain and antithrombin activity test results
The above experimental results demonstrate that the weight gain rate and survival rate of the two groups of examples 1 and 2 are significantly higher than those of the control group in the feeding period of 30 days. Wherein the survival rate is about 61 percent and 55 percent, the body weight is about 56 percent and 51 percent, and the antithrombin activity is 81.7 percent and 68.1 percent. The feed for feeding the poecilobdella manillensis can improve the immunity and disease resistance of the poecilobdella manillensis while preserving red blood cells completely, has better effects on the growth, the development, the propagation and the disease resistance of the poecilobdella manillensis, and has extremely high economic benefit when the feed for feeding the poecilobdella manillensis is applied.
Detailed Description
The examples are provided for the purpose of further illustrating the invention and are not to be construed as limiting the invention thereto.
Example 1:
the feed for feeding poecilobdella manillensis comprises the following raw materials in parts by weight: 80 parts of lyophilized pig blood powder, 10 parts of egg white, 0.6 part of calcium hydrophosphate and FeS040.2 part, 0.2 part of sodium chloride and trans-4, 5—Epoxy- (E) -2-decenal 0.3 parts, glutamic acid 3 parts, arginine 3 parts, lysine 4 parts, VitB10.5 part of VitB60.5 part and Vitc 1 part.
The preparation method comprises the following steps: weighing the materials according to the proportion, weighing, and mixing the materials according to the weight ratio of 1: adding drinking water at a ratio of 64, mixing, and loading into biological membrane.
Example 2:
the feed for feeding poecilobdella manillensis comprises the following raw materials in parts by weight: 70 parts of chicken blood freeze-dried powder, 7 parts of egg white, 0.5 part of calcium hydrophosphate and Fe (C)3H5O3)20.2 part of FeCl20.1 part, 0.1 part of sodium chloride and trans-4, 5—Epoxy- (E) -2-decenal 0.3 parts, arginine 2 parts, lysine 3 parts, VitB10.2 part of VitB20.2 part and VitE1 part.
The preparation method comprises the following steps: the same as in example 1.
Claims (4)
1. The feed for feeding poecilobdella manillensis is characterized by comprising the following raw materials in parts by weight: 60-80 parts of animal blood freeze-dried powder, 5-10 parts of egg white, 0.1-0.8 part of calcium hydrophosphate, 0.1-0.8 part of ferrous ion compound, 0.2-0.8 part of sodium chloride, trans-4, 5—0.2-0.5 part of epoxy- (E) -2-decenal, 1-10 parts of amino acid and 1-2 parts of vitamin.
2. The feed for feeding Poecilobdella manillensis as claimed in claim 1, wherein the iron (II) compound is FeS04、FeCl2、FeCO3、Fe(C3H5O3)2One or more of them.
3. The feed for feeding Poecilobdella manillensis as claimed in claim 1, wherein the amino acid is one or more of glutamic acid, arginine and lysine.
4. The feed for feeding Poecilobdella manillensis as claimed in claim 1, wherein the vitamin is VitB1、VitB2、VitB6One or more of Vitc and VitE.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010551542.3A CN111587967A (en) | 2020-06-17 | 2020-06-17 | Poecilobdella manillensis breeding feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
| CN115024440A (en) * | 2022-06-21 | 2022-09-09 | 楚雄师范学院 | Poecilobdella manillensis high-density culture feed and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1839689A (en) * | 2003-01-15 | 2006-10-04 | 周维海 | Aartificial breedubg method for P.manil-lensis |
| CN104351493A (en) * | 2014-09-30 | 2015-02-18 | 颍上县润海畜禽养殖有限公司 | Additive used for being adding into blood feed for leech breeding |
| CN105995237A (en) * | 2016-06-24 | 2016-10-12 | 苏州市泰盛农业科技有限公司 | Feed for culturing leeches and preparation method of feed |
| CN106063525A (en) * | 2016-06-24 | 2016-11-02 | 苏州市泰盛农业科技有限公司 | A kind of Hirudo raising diseases prevention improves immunity peplos feedstuff and preparation method thereof |
| CN107319211A (en) * | 2017-09-01 | 2017-11-07 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis young perfect compound feed and preparation method thereof |
| CN107348250A (en) * | 2017-09-01 | 2017-11-17 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis feed and preparation method thereof |
| CN109527270A (en) * | 2018-12-10 | 2019-03-29 | 苏州大学 | Leech specific nutrient induces the extracting method of liquid and natural hirudin |
-
2020
- 2020-06-17 CN CN202010551542.3A patent/CN111587967A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1839689A (en) * | 2003-01-15 | 2006-10-04 | 周维海 | Aartificial breedubg method for P.manil-lensis |
| CN104351493A (en) * | 2014-09-30 | 2015-02-18 | 颍上县润海畜禽养殖有限公司 | Additive used for being adding into blood feed for leech breeding |
| CN105995237A (en) * | 2016-06-24 | 2016-10-12 | 苏州市泰盛农业科技有限公司 | Feed for culturing leeches and preparation method of feed |
| CN106063525A (en) * | 2016-06-24 | 2016-11-02 | 苏州市泰盛农业科技有限公司 | A kind of Hirudo raising diseases prevention improves immunity peplos feedstuff and preparation method thereof |
| CN107319211A (en) * | 2017-09-01 | 2017-11-07 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis young perfect compound feed and preparation method thereof |
| CN107348250A (en) * | 2017-09-01 | 2017-11-17 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis feed and preparation method thereof |
| CN109527270A (en) * | 2018-12-10 | 2019-03-29 | 苏州大学 | Leech specific nutrient induces the extracting method of liquid and natural hirudin |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475624A (en) * | 2021-06-10 | 2021-10-08 | 云南农业大学 | Poecilobdella manillensis compound feed |
| CN115024440A (en) * | 2022-06-21 | 2022-09-09 | 楚雄师范学院 | Poecilobdella manillensis high-density culture feed and preparation method thereof |
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