CN109287881A - For improving the additive and its preparation method and application of cub immunity of organisms - Google Patents
For improving the additive and its preparation method and application of cub immunity of organisms Download PDFInfo
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Abstract
The present invention relates to a kind of for improving the additive and its preparation method and application of cub immunity of organisms.Wherein, for improving the additive of cub immunity of organisms, by weight percentage, comprising: herb fermenting object 10%~20%, oligoisomaltose 3%~10%, multi-vitamins 2%~12%, organic trace element 0.1%~4%, compound amino acid 1%~8% and surplus auxiliary material；The herb fermenting object is mainly prepared by the raw material of following parts by weight is fermented: 2~5 parts of Radix Glycyrrhizae, 8~12 parts of Cortex Eucommiae, 3~6 parts of fructus lycii, 5~9 parts of Radix Astragali, 5~8 parts of Poria cocos, 3~7 parts of purple perilla and 4~6 parts of motherwort.The immune function and intestinal health degree of cub can be remarkably reinforced for breeding infant livestock in weanling feed in the additive.
Description
Technical field
The present invention relates to poultry additive technology fields, more particularly to a kind of for improving adding for cub immunity of organisms
Add agent and its preparation method and application.
Background technique
With the development of Pig Industry industry, early weaning usually is carried out to promote breeding efficiency, piglet to piglet in production
Early weaning has become domestic and international intensive industrialized piggery and increases economic efficiency, reduces the most important means of production cost.However by
In piglet early stage alimentary canal not perfect enough, digestive juice and digestive ferment hyposecretion, micro ecology of gastrointestinal tract fauna it is unstable and
Intestinal immunity is low, and piglet also needs to be subjected to environment, psychology and nutrition and stress wait the influence of factors during weaning, because
This, in early weaning stage organism metabolism certain variation can occur for piglet, loss of appetite, indigestion, feeding such as usually occur
Expect that utilization rate is low, under immunity and premunition phenomena such as grand, diarrhea, be eventually exhibited as growth inhibition, i.e., so-called " weaned piglet
Syndrome ".
The main reason for generating this series of symptoms is variation and cause of disease of the piglet by nutrition, environment and psychology
Intestine of young pigs impaired development caused by microbiological attack, function of intestinal canal disorder, so as to cause production performance reduction, diarrhea and death
Rate rises, and seriously threatens the life and health of piglet, then brings massive losses to pig production.Enteron aisle is not only to digest and assimilate
The main place and body of nutriment resist exogenous and endogenous pathogenic bacteria the first line of defences, are that body is maximum
Immune organ.The performance of intestinal health level decision animal holistic health and production performance.Due to intestine of young pigs development, siberian crabapple
System, enzyme system, microecosystem etc. are unsound, in addition piglet intestinal canal barrier caused by weaning stress damages, cause grice diarrhoea,
Production performance reduces, or even dead, the serious development for restricting pig breeding industry.Therefore, prepare be suitble to early-weaned piglets feed or
Feed addictive is the most effectual way for overcoming early weaning syndrome.
Summary of the invention
Based on this, it is necessary to provide a kind of additive, the immunity of organisms of cub can be enhanced.
It is a kind of for improving the additive of cub immunity of organisms, by weight percentage, comprising: herb fermenting object 10%
~12%, oligoisomaltose 3%~10%, multi-vitamins 2%~12%, organic trace element 0.1%~4%, compound
Amino acid 1 %~8% and surplus auxiliary material；
The herb fermenting object is mainly prepared by the raw material of following parts by weight is fermented: 2~5 parts of Radix Glycyrrhizae, Cortex Eucommiae 8~
12 parts, 3~6 parts of fructus lycii, 5~9 parts of Radix Astragali, 5~8 parts of Poria cocos, 3~7 parts of purple perilla and 4~6 parts of motherwort.
It is above-mentioned for improving the additive of cub immunity of organisms, by by herb fermenting object, oligoisomaltose, compound
Vitamin, organic trace element and compound amino acid make an addition in cub feed after carrying out scientific compatibility as feed addictive,
Each ingredient complements each other, and can cooperate with the body's immunity of enhancing cub, moreover it is possible to improve cub intestine microenvironment, ensure children
The intestinal health of poultry is developed, and effect stability is simultaneously substantially better than generated effect when only adding a kind of ingredient.Secondly, above-mentioned add
Add materials safety selected by agent nontoxic, make an addition in pig starter feed, combined immune function and the enteron aisle that cub can be remarkably reinforced are strong
Health.
Herb fermenting object, by Radix Glycyrrhizae, Cortex Eucommiae, fructus lycii, Radix Astragali, Poria cocos, purple perilla and motherwort is fermented is prepared, rich
Containing active polysaccharide, alkaloid, flavones, saponin(e and chlorogenic acid isoreactivity ingredient, has and promote T lymphocyte and bone-marrow-derived lymphocyte
Proliferation enhances the activity of macrophage and monocyte；Enhance hematopoiesis function；It can also accelerate the growth of beneficial bacteria of intestinal tract, press down
The growth of harmful intestinal tract bacteria processed, protection intestinal health development.
Oligoisomaltose is one kind of starch sugar, what main component was combined between glucose molecule with α -1,6 glycosidic bonds
Oligosaccharide more than isomaltose, panose, Isomaltotriose and tetrose.Oligoisomaltose will not by stomach and small intestinal absorption, and
It is to be directly entered large intestine, is preferentially utilized by Bifidobacterium, help its proliferation, and the other harmful bacterias of enteral cannot then utilize, so as to
Inhibit harmful bacteria growth, Tiny ecosystem in enteron aisle is promoted to adjust to benign cycle, so, oligoisomaltose can promote in enteron aisle
Bifidobacterium proliferation, inhibits harmful intestinal tract bacteria and corrupt substance to be formed, and increases vitamin content, improves immunity of organisms.
Multi-vitamins are the composite dosage form vitamin that multivitamin is synthesized according to doses ratio, generally comprise
Liposoluble vitamin (vitamin A, vitamin D, vitamin E, vitamin K) and water soluble vitamin (B family vitamin, vitamin
C).Necessary nutritional ingredient can be provided for animal, have and improve immunity of organisms and free radical resisting oxidation, promote growth, side
Aid digestion, improvement mental status, promotes the effect of resisting stress capability.
Organic trace element refers to inorganic salts and organic matter and its decomposition of the microelements such as manganese, iron, copper, zinc, selenium and iodine
The compound that product is formed.Microelement is nutrient necessary to animal body, participate in animal body in enzyme and hormone generation with
Activation, the directly almost all of physiology of participation body and biochemical function, have the growth and development whole process of cub extremely heavy
The influence wanted.And organic trace element biological value is high, utilization rate is high, and a small amount of addition can replace the inorganic salts of high dose,
The discharge rate of animal microelement can also be reduced, the pollution to environment is reduced.
Compound amino acid is made of a variety of amino acid.Amino acid is the organic compound containing basic amine group and acidic carboxypolymer
The base substance of protein needed for object, and composition Animal nutrition, presence of the amino acid in body provide not only synthesis egg
The important source material of white matter, and for promoting to grow, progress eubolism, sustaining life provides material base.Compounded amino
Acid is capable of providing a variety of amino acid required for piglet development, moreover it can be used to synthetic antibody.
In one of the embodiments, by weight percentage, the additive include: herb fermenting object 12%~
15%, oligoisomaltose 5%~10%, multi-vitamins 2%~12%, organic trace element 2%~3%, compounded amino
Acid 1%~8% and surplus auxiliary material.
It is appreciated that above-mentioned additive in can also include except herb fermenting object, oligoisomaltose, multi-vitamins,
Other traditional Chinese medicinal components and nutritional ingredient other than organic trace element and compound amino acid.
The raw material of the herb fermenting object includes: 4 parts of Radix Glycyrrhizae, 10 parts of Cortex Eucommiae, fructus lycii 5 in one of the embodiments,
Part, 7 parts of Radix Astragali, 6 parts of Poria cocos, 5 parts of purple perilla and 5 parts of motherwort.
The organic trace element is the glycine complexes of iron or ferrous iron in one of the embodiments,.It is appreciated that
Iron can participate in synthesis hemoglobin and myoglobins, promote the transport of oxygen, get up to prevent piglet anaemia, improve the work of immunization rate
With, it is added by the glycine complexes form of ferrous glycine, ferrous bisglycinate chelate and/or iron (ferrous iron), it can be by glycine
It absorbs, to promote absorption and utilization of the animal to ferro element, to promote the synthesis of hemoglobin and myoglobins, prevents iron deficiency
Property anemia, promotes the growth and development of piglet, keeps its skin ruddy, hair color is shinny, and improves the immunity of piglet.
It in one of the embodiments, include vitamin A, vitamin D, vitamin E, vitamin in the multi-vitamins
K, at least two in B family vitamin and vitamin C.
It in one of the embodiments, include lysine, tryptophan, methionine, threonine, figured silk fabrics in the compound amino acid
Propylhomoserin and arginine.
The auxiliary material includes glidant and diluent in one of the embodiments, and the glidant accounts for the additive
The 0.5%~3% of total weight.
In one of the embodiments, the glidant in silica, magnesium stearate and talcum powder at least one
Kind., can be unimpeded even into blender in this way, the glidant of addition 0.5%~3%, is able to maintain the wandering of additive raw material,
To guarantee the quality of additive after mixing.
In one of the embodiments, the diluent in starch, defatted rice bran, bean cake powder and glucose at least
It is a kind of.In this way, selection animal is edible and the higher starch of innocuous substance, nutritive value, defatted rice bran, bean cake powder etc. are as dilute
Agent is released, is added in additive, the concentration of the components such as herb fermenting object, oligoisomaltose can be reduced, and by a variety of work
Property component particle separate, the mixing uniformity of additive and the quality of the subsequent premix with feed is effectively ensured.
In one of the embodiments, in order to improve the palatability of product, the auxiliary material further includes fragrance.
It is appreciated that above-mentioned additive is since the herb fermenting object that is prepared by fermentations such as Radix Glycyrrhizae, Radix Astragalis has fragrance,
And oligoisomaltose makes additive have sweet taste appropriate, therefore, product odour is fragrant, and palatability is good.But in order to make to add
The palatability for adding agent more preferably, can also add suitable fragrance.
Another object of the present invention is to provide above-mentioned for improving the preparation method of the additive of cub immunity of organisms, packet
Include following steps:
According to the formula of the additive, each component is weighed, each component is uniformly mixed, obtains the additive.
Above-mentioned preparation method is easy to operate, and preparation condition controllability is good, can mass large-scale production.
In one of the embodiments, before the step of weighing each component, by each component raw material in the additive
Crush and sieving is handled, the aperture of sieve pore is 40~80 mesh in the sieving processing.
Further include the steps that preparing the Chinese medical extract in one of the embodiments:
The raw material of the herb fermenting object is crushed and is uniformly mixed, mixed powder is obtained；
It is 1:(40~50 according to the mass ratio of the mixed powder and water), the mixed powder and water are mixed, sterilizing,
After cooling, be inoculated with the probiotics strain of activation, in temperature is 36 DEG C~38 DEG C, pH value is 5.8~6.5, pressure be 0.02kPa~
Ferment 40h~50h under conditions of 0.05kPa, obtains fermenting mixture；
The fermenting mixture is filtered, collects filtrate and filter residue respectively；
By the filtrate in 50 DEG C~65 DEG C progress spray drying treatments, then and by drying, the institute after pulverization process
It states filter residue to be uniformly mixed, obtains the herb fermenting object.
In this way, by filtering fermenting mixture, filter residue and filtrate remix after being individually dried, and wherein filtrate is in 50
DEG C~65 DEG C of progress spray drying treatments, it was not only able to maintain the activity of active constituent, but also be able to maintain the activity of probiotics, piglet is edible
After above-mentioned additive, probiotics can in piglet stomach growth and breeding, make up the deficiency of piglet gastric acid to a certain extent, make son
PH maintains reduced levels in pig stomach, inhibits bacteriums growth and breedings in stomach such as Escherichia coli, salmonella, clostridium.
The probiotics is selected from lactobacillus acidophilus, De Shi lactic acid bar, lactobacillus plantarum, excrement in one of the embodiments,
At least one of streptococcus and enterococcus faecium.
Preferably, the probiotics is lactobacillus acidophilus.
Yet another object of that present invention is to provide application of the above-mentioned additive in preparation cub feed.
In one of the embodiments, by the additive application in the feed preparation of piglet.
The additive 0.1%~0.5% is added to piglet feeding by mass percentage in one of the embodiments,
In material.
Further, the additive 0.1%~0.5% is added to the Piglet at Lactation Period feed by mass percentage
In, to improve the immunity and intestinal health degree of piglet.
Above-mentioned additive application in the feed of weanling pig and is fed into piglet, the immunity of piglet can be significantly improved
With the microecosystem for improving piglet gastrointestinal tract, enhance intestine of young pigs health degree, improves piglet survival ratio.
Specific embodiment
To facilitate the understanding of the present invention, below will to invention is more fully described, and give it is of the invention compared with
Good embodiment.But the invention can be realized in many different forms, however it is not limited to embodiment described herein.Phase
Instead, purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
The following are specific embodiments.
The embodiment of the present invention is raw materials used derive from it is commercially available.
Wherein, multi-vitamins include vitamin A, vitamine D3, vitamin E, Vitamin K3, vitamin C, vitamin
B2, vitamin B6 and raw plain element B12；Compound amino acid includes lysine, methionine, tryptophan, threonine, arginine and figured silk fabrics ammonia
Acid.
The formula of the additive of the raising pigling immunity of Examples 1 to 5, see the table below 1.
The formula of 1 additive of table
Note: "-" expression is not added；Glidant uses silica in table, and diluent uses glucose；Certain glidant is also
Magnesium stearate can be selected or defatted rice bran, bean cake powder or starch can be selected in talcum powder, diluent.
2, preparation method:
A, the preparation step of Chinese medical extract is as follows:
1) 4 portions of Radix Glycyrrhizaes, 10 parts of Cortex Eucommiaes, 5 portions of fructus lyciis, 7 parts of Radix Astragalis, 6 parts of Poria cocos, 5 parts of purple perillas and 5 parts of benefits, are accurately weighed
Brittle Falsepimpernel Herb crushes after mixing, crosses 60 meshes, obtains mixed-powder, for use；
2), picking lactobacillus acidophilus 37 DEG C of culture 36h in solid medium are activated；
Solid culture based formulas: peptone 10g, powdered beef 5g, yeast powder 4g, glucose 20g, Tween 80 1mL, phosphoric acid
Hydrogen dipotassium 2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 0.2g, manganese sulfate 0.05g, agar 18g and pure water 1000mL.
3), the lactobacillus acidophilus of picking activation is in fluid nutrient medium, and 37 DEG C of static gas wave refrigerator 36h are expanded in incubator
Training；
Fluid nutrient medium: peptone 10g, powdered beef 5g, yeast powder 4g, glucose 20g, 1mL Tween 80, dipotassium hydrogen phosphate
2g, sodium acetate 5g, Triammonium citrate 2g, magnesium sulfate 0.2g, manganese sulfate 0.05g and pure water 1000mL.
4), the mixed-powder and water by the poly- 1) preparation of step are added in fermentor according to weight ratio 1:50, and fermentation is added
Culture medium, fermentor lead to steam sterilizing 30min, inoculation step 3) lactobacillus acidophilus spread cultivation bacterium solution, inoculum concentration 8vol%, in
37 DEG C, 48 ± 2h of 20r/min, 0.03kpa culture.
Fermentation medium: beancake powder 15g/L, whey powder 10g/L, beef extract 12g/L, peptone 16g/L, corn starch
20g/L, glucose 20g/L, magnesium sulfate 0.5g/L, manganese sulfate 0.25g/L, sodium acetate 7g/L, dipotassium hydrogen phosphate 2g/L, pH6.2.
5), fermentation finishes, and puts tank and collects fermentation liquid, collects filtrate and filter residue respectively in plate-frame filtering fermentation liquid, filtrate in
Nozzle diameter is 0.5mm~1mm, and the low temperature spray drying machine that temperature is 50 DEG C~65 DEG C is dry, and filter residue is 60 in convection oven
DEG C~80 DEG C of baking 4h~5h after crush, the two merge, mix to get Chinese medical extract.
B, the preparation step for improving the additive of pigling immunity is as follows:
Graininess and block-like raw material are crushed respectively, cross pharmacopeia 2 sieves (being equivalent to 24 mesh of sieve pore)；
The raw material components after respectively pulverizing and sieving are accurately weighed according to the formula of each embodiment and comparative example in table 1；
Each raw material component is uniformly mixed, pharmacopeia 3 sieves (being equivalent to 50 mesh of sieve pore), packing are crossed.
It is pulvis by the additive that the above method is prepared, fragrant odour, palatability are good, and are convenient for subsequent addition
Mixing.
Effect proof property test
1, test material
The additive of above-described embodiment 1.
2, experimental animal and experimental design
It is designed using single factor experiment, selects the sow of 30 gestation 1d to be tested according to parity, body condition phase approximately principle,
Separated raising.Agent of feed for pregnancy sow is fed since sow gestation 1d, and is continued until that pregnant 90d changes milking sow into again
Feed is to lactation 21d (weaned piglet) off-test.By the principle of 7 age in days nest uniform quality of sow litter, by 30 nests
Piglet is divided into 3 test groups, every group of 10 nest piglets.
Piglet is tested since when starting 7 ages in days addition creep feed, wherein testing I group as a control group；Test II group
According to the additive of the ratio addition embodiment 1 of 2000g/t feed in the creep feed of piglet；Test III group of creep feed in piglet
According to 3000g/t feed ratio addition embodiment 1 additive.
3, feeding management
After Farrowing, piglet can intersect in group according to weight size to be entrusted one's child to the care of sb. and (completes within 12h after Farrowing),
Guarantee every litter size amount (10 or 11) and weight is evenly distributed；The suckling pig childbirth same day cuts tail, removes canine tooth and mends iron, point
Puerperium 7d starts piglet castrating and religion slot, warming using 250 watts of infrared heat-insulating lamps.Experimental period 7-21 age in days, feeds 14d altogether.
Experimental period piglet 07:00 every morning, morning 10:00, noon 12:00,15:00 in afternoon, evening 18:00 and evening 21:00 etc. 6
A time point timing feeding, a small amount of multiple principle.Other feeding managements and immune programme follow the progress of pig farm management system.Often
Its record temperature, humidity, observe and record sow health status.
4, piglet basal diet composition and trophic level, see the table below 2.
Table 2
Note: premix provides for every kilogram of feed: vitamin A 12000IU, vitamine D3 2500IU, vitamin E
30IU, vitamin K 3mg, vitamin B1 1.52mg, vitamin B2 4mg, vitamin B6 3mg, vitamin B12 0.012mg,
Pantothenic acid 15mg, niacin 40mg, choline chloride 400mg, zinc 80mg, iron 84mg, manganese 20mg, copper 180mg, iodine 0.48mg, selenium
0.36mg and aureomycin 100mg.
5, measurement item
10 piglet (every nests 1 similar in 14 age in days of piglet and 21 ages in days (the wean same day) every group selection health degree
Head), piglet is fixed up, whole body in line, is lifted on forelimb, by needle from two alveoles of chest sideling to opposite side shoulder joint
It saves top to be pierced into, vena cava anterior sinus blood sampling 20ml is directly looked for, wherein 10ml anticoagulant heparin, for measuring CD4+、CD8+Lymph is thin
Born of the same parents' content and its ratio；In addition 10ml stands 10-15min, is carried out centrifugation 15min (3500r/min) using centrifuge, in absorption
Clear liquid-serum is sub-packed in the EP pipe of 1.5ml, the freezen protective in -20 DEG C of refrigerators, for measuring Immunoglobulin IgG, IgM
Concentration.
It first weighs in 21 age in days of piglet and draws blood again, blood drawing finishes that venesection is lethal, butchers, pancreatic tissue is completely taken out,
And accurate weighing pancreas weight.Take duodenum 2cm, jejunum 2cm, ileum 2cm, after PBS buffer solution cleaning, be placed in 4% it is more
It is fixed in case histological observation and analysis in polyformaldehyde solution.
6, test data is handled using SPSS software, and indices see the table below 3~6.
Influence (μ g/mL) of the table 3 to piglet Immunoglobulin in Serum concentration
Note: letter is different between different groups indicates significant difference (P < 0.05), not significant (the P > of identical expression difference
0.05)。
Immunoglobulin is with antibody activity or chemical structure globulin similar with antibody, is to receive to resist by B cell
Proliferation, Differentiation is the important immune molecule of humoral immunity caused by thick liquid cell after primary stimuli.IgG has antibacterium, disease
The effect of the various actives such as poison, exotoxin, promotes the phagocytosis of mononuclear macrophage；IgM have sterilization, bacteriolyze, promote swallow and
Agglutination has powerful anti-infectious function.
As can be seen from Table 3, in experimental period, II group and test III group of piglet Immunoglobulin in Serum IgG and IgM are tested
Compared with control group (I group of test), be significantly increased (P < 0.05), and showing can be significant by the additive for feeding the embodiment of the present invention
The content for improving piglet Immunoglobulin in Serum, illustrates that the immunity of piglet is significantly improved.
Table 4 is to CD4 in piglet blood+And CD8+The influence (%) of lymphocyte content
Note: letter is different between different groups indicates significant difference (P < 0.05), not significant (the P > of identical expression difference
0.05)。
CD4+It is the molecule of a transmitting signal, promotes t cell activation.CD8+Major function removing is infected
Cell, CD8+Content is higher, shows that infected cell is more, illustrates that the immunocompetence of body itself reduces.CD4+With CD8+Than
The reduction of value is the important indication of immunocompetence decline.
As can be seen from Table 4, in experimental period, CD4 in III group of piglet blood of II group and test is tested+Content is compared with control group
(I group of test) is significantly raised, and CD8+Content is decreased obviously compared with control group, CD4 +/CD8 +Value have compared with control group (test I group)
It significantly improves, illustrates that feeding additive of the embodiment of the present invention can promote to activate the T cell of piglet, removing is infected cell
Ability enhancing, the resistance and immunity of piglet can be improved.
Influence of the table 5 to weanling pig pancreas quality
Note: letter is different between different groups indicates significant difference (P < 0.05), not significant (the P > of identical expression difference
0.05)。
As can be seen from Table 5, it feeds 21 days, test II group and tests the pancreas weight and pancreas relative mass of III group of piglet
There is significant difference compared with test I group (blank control group), pancreas weight and pancreas relative mass are all remarkably higher than test I group, examination
The living body weight of III group of piglet of II group and test is tested also above test I group, show to feed 1 additive of the embodiment of the present invention can be bright
The aobvious pancreatic tissue growth for promoting piglet, while the growth and development of piglet can be promoted；And the juice of pancreas is egg in small intestine
White catabolic enzyme, amylolytic enzyme and lipolysis main source, can illustrate to feed 1 additive of the embodiment of the present invention indirectly can be with
Enhance the digestion power to feed of piglet.
Influence of the table 6 to weanling pig enteron aisle structure
Note: the hidden ratio than being height of naps and Crypt depth of suede；Letter is different between different groups indicates significant difference (P
< 0.05), identical expression difference is not significant (P > 0.05).
Villus is the functional unit of small intestine, and villus, which is got higher, substantially increases the absorption area of nutriment.And crypts becomes
Shallowly, show that the maturing rate of cell rises, secreting function is strong.Suede it is hidden than can concentrated expression small intestine functional status, the petition of surrender under ratio
Bright digestion and absorption function decline, often with the generation of diarrhea disease；Ratio rises, digestion and absorption function enhancing, diarrhea rate decline.
As can be seen from Table 6, it after feeding 21 days, tests III group of intestine of young pigs of II group and test and (duodenum, jejunum and returns
Intestines) height of naps, Crypt depth, suede it is hidden than all having notable difference compared with test I group (not using any additive),
Feed III group of intestine of young pigs (duodenum, jejunum and ileum) of 1 additive of the embodiment of the present invention, 21 days test II groups and test
Height of naps be apparently higher than test I group, and Crypt depth be substantially less than test I group, suede it is hidden than be significantly higher than test I group, table
Small intestinal villous height and the hidden ratio of suede can be improved in bright feeding 1 additive of the embodiment of the present invention, can be obviously improved weanling pig
The alimentary canal shape of enteron aisle illustrates that the additive of the embodiment of the present invention 1 can significantly improve the intestinal health degree of piglet.
Referring to said effect prove property test method, not add any additive as blank control group, and respectively with
The additive of embodiment 2~5 and comparative example 1~2 is that test material is tested, and the additive amount of the additive of embodiment 2~5 is
3000g/t, as the result is shown: comparing blank control group, the piglet of 2~5 additive of embodiment is added in feeding, immune in serum
Globulin IgG and IgM are improved significantly, CD4 in blood+Content is significantly raised, CD8+Content is decreased obviously, CD4 +/CD8 +
Value increase, show that the immunity of weanling pig can be improved in the additive for feeding the embodiment of the present invention 2~5；The feeding present invention
2~5 additive of embodiment 21 days, the living body of piglet are higher than the control group for not feeding any additive again, and the pancreas weight of piglet
Control group is all remarkably higher than with pancreas relative mass and feeds the test group of comparative example 1 and 2 additive of comparative example, the intestines of piglet
The height of naps in road (duodenum, jejunum and ileum) is apparently higher than control group and feeds the test of 1~2 additive of comparative example
Group, Crypt depth are then significantly lower than control group and comparative example 1~2.
And the piglet of addition 1~2 additive of comparative example is fed, compared with control group, though the immunoglobulin content in serum
It so increases, but effect is unobvious, and CD4 in piglet blood+Content increases unobvious, CD8+Content is declined, CD4 +/
CD8 +Value increase it is not significant, illustrate comparative example 1~2 to improve pigling immunity it is ineffective；Feeding 21 days, piglet
Pancreas weight and pancreas relative mass are also unobvious compared with increasing for control group, while intestine of young pigs health degree improvement is also unknown
It is aobvious.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of for improving the additive of cub immunity of organisms, which is characterized in that by weight percentage, comprising: Chinese medicine
Fermentation material 10%~20%, oligoisomaltose 3%~10%, multi-vitamins 2%~12%, organic trace element 0.1%
~4%, compound amino acid 1%~8% and surplus auxiliary material；
The herb fermenting object is mainly prepared by the raw material of following parts by weight is fermented: 2~5 parts of Radix Glycyrrhizae, Cortex Eucommiae 8~12
Part, 3~6 parts of fructus lycii, 5~9 parts of Radix Astragali, 5~8 parts of Poria cocos, 3~7 parts of purple perilla and 4~6 parts of motherwort.
2. according to claim 1 for improving the additive of cub immunity of organisms, which is characterized in that percentage by weight
Than meter, the additive includes: herb fermenting object 12%~15%, oligoisomaltose 5%~10%, multi-vitamins 2%
~12%, organic trace element 2%~3%, compound amino acid 1%~8% and surplus auxiliary material.
3. according to claim 1 for improving the additive of cub immunity of organisms, which is characterized in that the Chinese medicine hair
The raw material of ferment object includes: 4 parts of Radix Glycyrrhizae, 10 parts of Cortex Eucommiae, 5 parts of fructus lycii, 7 parts of Radix Astragali, 6 parts of Poria cocos, 5 parts of purple perilla and 5 parts of motherwort.
4. according to claim 1 for improving the additive of cub immunity of organisms, which is characterized in that described organic micro-
Secondary element is the glycine complexes of iron or ferrous iron.
5. according to any one of claims 1 to 4 for improving the additive of cub immunity of organisms, which is characterized in that
The auxiliary material includes glidant and diluent, and the glidant accounts for the 0.5%~3% of the additive total weight.
6. according to claim 5 for improving the additive of cub immunity of organisms, which is characterized in that the glidant
Selected from least one of silica, magnesium stearate and talcum powder；And/or
The diluent is selected from least one of starch, defatted rice bran, bean cake powder and glucose.
7. claim 1~6 is described in any item for improving the preparation method of the additive of cub immunity of organisms, feature
It is, comprising the following steps:
According to the formula of the additive, each component is weighed, each component is uniformly mixed, obtains the additive.
8. according to claim 7 for improving the preparation method of the additive of cub immunity of organisms, which is characterized in that
Further include the steps that preparing the herb fermenting object:
The raw material of the herb fermenting object is crushed and is uniformly mixed, mixed powder is obtained；
It is 1:(40~50 according to the mass ratio of the mixed powder and water), the mixed powder and water are mixed, sterilizing, cooling
Afterwards, be inoculated with the probiotics strain of activation, in temperature is 36 DEG C~38 DEG C, pH value is 5.8~6.5, pressure be 0.02kPa~
Ferment 40h~50h under conditions of 0.05kPa, obtains fermenting mixture；
The fermenting mixture is filtered, collects filtrate and filter residue respectively；
By the filtrate in 50 DEG C~65 DEG C progress spray drying treatments, then and by drying, the filter after pulverization process
Slag is uniformly mixed, and obtains the herb fermenting object.
9. according to claim 8 for improving the preparation method of the additive of cub immunity of organisms, which is characterized in that
The probiotics in lactobacillus acidophilus, De Shi lactic acid bar, lactobacillus plantarum, streptococcus fecalis and enterococcus faecium at least one
Kind.
10. application of the described in any item additives of claim 1~6 in preparation cub feed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811444044.8A CN109287881A (en) | 2018-11-29 | 2018-11-29 | For improving the additive and its preparation method and application of cub immunity of organisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811444044.8A CN109287881A (en) | 2018-11-29 | 2018-11-29 | For improving the additive and its preparation method and application of cub immunity of organisms |
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| CN109287881A true CN109287881A (en) | 2019-02-01 |
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| CN201811444044.8A Pending CN109287881A (en) | 2018-11-29 | 2018-11-29 | For improving the additive and its preparation method and application of cub immunity of organisms |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112401056A (en) * | 2020-10-29 | 2021-02-26 | 江苏弘康生物科技有限公司 | Animal feed additive and use method thereof |
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Application publication date: 20190201 |