CN104904674A - Breeding method for young leeches - Google Patents
Breeding method for young leeches Download PDFInfo
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- CN104904674A CN104904674A CN201510343672.7A CN201510343672A CN104904674A CN 104904674 A CN104904674 A CN 104904674A CN 201510343672 A CN201510343672 A CN 201510343672A CN 104904674 A CN104904674 A CN 104904674A
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- leech
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a breeding method for young leeches. The breeding method comprises the steps of preparing a hatching box, hatching the young leeches, raising and training the young leeches and the like. The breeding method is simple and easy to perform, health growing of the leeches is guaranteed, the nutritional structure in the leech body can be improved, and the medicinal value is improved.
Description
Technical field
The present invention relates to a kind of aquaculture method, be specially the raising method of a kind of leech seedling.
Background technology
Leech, have another name called leech, it is a kind of valuable traditional Chinese medicine, for the famous and precious medicinal animal of China's tradition, " record in the medical science allusion quotation such as Compendium of Materia Medica > work: leech treatment apoplexy, hypertension have good curative effect, and " hirudin " that contain in leech glandula is the special specific medicament of anti-curing cancers; Hirudin containing the solid bacterium of a kind of anti-freezing in fresh leech saliva can resist the thrombosis of ADP induction, and energy activated cellulose system, promote that established thrombus is dissolved; Leech also secretes a kind of histamine compound matter, can expand capillary.Current leech is the Chinese patent drug that primary raw material is made, and dropped into a large amount of production, and supply falls short of demand.
Existing cultural technique, raiser often directly buys blood product and feeds leech, but leech food amount is few, poor growth, and the culture-cycle is long, not comprehensive nutrition, easily causes the generation of the situation of dying of illness.
Summary of the invention
The invention provides the raising method of a kind of leech seedling, the method is simple, while ensureing the healthy growth of leech, can also improve the trophic structure in leech body, increases medical value.
For achieving the above object, technical scheme of the present invention is as follows:
A raising method for leech seedling, comprises the following steps:
(A) incubator box is prepared:
Select the uncovered casing of plastics or wooden materials, place that one deck 2-3cm is thick and humidity is the rough sand of 30-40% at bottom half, be that the non-woven fabrics matrix of 1.5-2.5cm is laid on above-mentioned rough sand layer by thickness, soil humidity is 30-40%; The thickness that matrix is laid bottom surface and wall is the latticed water suction moisturizing layer of 0.5-1.5cm, the sizing grid of water suction moisturizing layer puts into one piece of Whitmania pigra Whitman spawns for suitable, moisturizing layer humidity 30-40%, incubator box top is covered with black fabric, in casing, the humidity of air remains on 75-85%, and wall box is hung with the Hygrothermograph for monitoring case environment at any time;
(B) hatching of seedling:
B1, selective body focus on the physically fit adult kind leech of 18-22g/ bar, put into glass nursery cylinder and lay eggs after sterilization, to control in nursery cylinder water temperature at 20 DEG C, and leech mating also pierces side in cylinder and to loosen the soil middle product cocoon;
After B2, leech lay eggs 9-11 days start to artificially collect, and get the grid that the ovum cocoon given birth in 3 days is placed in above-mentioned casing, temperature is set to 20-25 DEG C, treats young leech cocoon-break;
B3, hatch out through 5-10 days leech seedling;
(C) the raising and train of leech seedling
C1, wide-aperture for 300 × 250 × 30mm specification sponge to be immersed in liquid feed 1 hour, to allow sponge fully receive liquid feed;
C2, by the leech seedling just having hatched out according to constant density be put into bottom be placed with in the glass nursery cylinder of sponge, and above nursery cylinder, hide gauze so as not to leech seedling escape;
C3, every day in the morning 7 and allow leech seedling suck each 2 hours at 11 in evening on sponge respectively, and adopt water spray means to keep nursery cylinder humidity;
C4, each leech suck the stimulation of rear use 30% salt solution and are all brushed down from sponge upper punch by leech, then fish for out by leech seedling with nylon mesh screen immediately, discard salt solution, and cleaned by sponge for subsequent use;
B5, the leech pulled out move to respectively and are equipped with in the nursery cylinder of depth of water 15cm in addition, and the top of nursery cylinder is provided with anti-escape net, and cobble is put into as base in bottom, every morning 10 and 8 oxygenations in evening 2 times, each 0.5 hour;
C6, repeat the feeding process of C3-C5 every day, change the liquid feed in sponge every day.
In described step (A), the light transmittance of the black fabric of hatching initial stage employing is 10-20%, and the light transmittance of the black fabric of hatching employing in mid-term is 20-30%, and the light transmittance of the black fabric that the hatching later stage adopts is 30-60%.
The grid of water suction moisturizing layer is square, rhombus or circle in described step (A).
In described step (C), liquid feed is made up of 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal, 5-7g spiral shell meat, 3-7g saccharomycete and distilled water 500ml.
The preparation method of described liquid feed is: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal, 5-7g spiral shell meat and 3-7g saccharomycete are fully mixed rear adding distil water to 500ml, and adjust pH is 7.0.
In described step C2, the density of leech seedling is 1500.
Described glass nursery cylinder specification is 40 × 30 × 20cm.
Nylon mesh screen in described step C4 is 80 order nylon mesh screens.
In described step C5, water body pH is between 6 ~ 7, and water temperature remains between 20 ~ 30 DEG C, and dissolved oxygen amount is greater than 8 mg/litre.
The leech seedling that described raising method is raised is eurysome golden thread leech.
Beneficial effect of the present invention is:
Technical scheme of the present invention can effectively avoid the natural enemy such as bird, fish under nature incubation condition to the predation of the leech young, the conditions such as strong daylight, heavy rain are to the restriction of hatching Whitmania pigra Whitman spawns, Whitmania pigra Whitman spawns is hatched under comparatively stable condition, can effectively improve hatching speed and hatchability, unified brooding time, effectively can reduce the difference of hatchling, be beneficial to centralized management and gather, improving utilization of resources rate.
The liquid feed of leech seedling of the present invention and use this liquid feed to raise the method for leech seedling, seedling to young seedling can be made to shorten to 20 days vegetative period, average individual body weight is about 0.28g/ bar, and survival rate remains on more than 96%, use the raising method of liquid feed of the present invention can reach the quality and quantity of best commodity seedling.
Embodiment
More being convenient to make content of the present invention understand, below in conjunction with embodiment, technical solutions according to the invention are described further, but the present invention being not limited only to this.
Embodiment 1
The raising method of leech seedling of the present invention, comprises the following steps:
(A) incubator box is prepared:
Select the uncovered casing of plastics or wooden materials, place that one deck 2cm is thick and humidity is the rough sand of 30% at bottom half, be that the non-woven fabrics matrix of 1.5cm is laid on above-mentioned rough sand layer by thickness, soil humidity is 30%; The thickness that matrix is laid bottom surface and wall is the latticed water suction moisturizing layer of 0.5cm, the sizing grid of water suction moisturizing layer puts into one piece of Whitmania pigra Whitman spawns for suitable, moisturizing layer humidity 30%, incubator box top is covered with black fabric, in casing, the humidity of air remains on 75%, and wall box is hung with the Hygrothermograph for monitoring case environment at any time;
(B) hatching of seedling:
B1, selective body focus on the physically fit adult kind leech of 18g/ bar, put into glass nursery cylinder and lay eggs after sterilization, to control in nursery cylinder water temperature at 20 DEG C, and leech mating also pierces side in cylinder and to loosen the soil middle product cocoon;
B2, leech lay eggs and start to artificially collect for latter 9 days, and get the grid that the ovum cocoon given birth in 3 days is placed in above-mentioned casing, temperature is set to 20 DEG C, treats young leech cocoon-break;
B3, hatch out through 5 days leech seedling;
(C) the raising and train of leech seedling
C1, wide-aperture for 300 × 250 × 30mm specification sponge to be immersed in liquid feed 1 hour, to allow sponge fully receive liquid feed;
C2, by the leech seedling just having hatched out according to constant density be put into bottom be placed with in the glass nursery cylinder of sponge, and above nursery cylinder, hide gauze so as not to leech seedling escape;
C3, every day in the morning 7 and allow leech seedling suck each 2 hours at 11 in evening on sponge respectively, and adopt water spray means to keep nursery cylinder humidity;
C4, each leech suck the stimulation of rear use 30% salt solution and are all brushed down from sponge upper punch by leech, then fish for out by leech seedling with nylon mesh screen immediately, discard salt solution, and cleaned by sponge for subsequent use;
B5, the leech pulled out move to respectively and are equipped with in the nursery cylinder of depth of water 15cm in addition, and the top of nursery cylinder is provided with anti-escape net, and cobble is put into as base in bottom, every morning 10 and 8 oxygenations in evening 2 times, each 0.5 hour;
C6, repeat the feeding process of C3-C5 every day, change the liquid feed in sponge every day.
In described step (A), the light transmittance of the black fabric of hatching initial stage employing is 10%, and the light transmittance of the black fabric of hatching employing in mid-term is 20%, and the light transmittance of the black fabric that the hatching later stage adopts is 30%.
The grid of water suction moisturizing layer is square, rhombus or circle in described step (A).
In described step (C), liquid feed is made up of 200ml cow's serum, 30g raw egg, 30g glucose, 10g dregs of beans, 1g calcium carbonate, 0.01%w/v B B-complex, 5g blood meal, 5g spiral shell meat, 3g saccharomycete and distilled water 500ml.
The preparation method of described liquid feed is: 200ml cow's serum, 30g raw egg, 30g glucose, 10g dregs of beans, 1g calcium carbonate, 0.01%w/v B B-complex, 5g blood meal, 5g spiral shell meat and 3g saccharomycete are fully mixed rear adding distil water to 500ml, and adjust pH is 7.0.
In described step C2, the density of leech seedling is 1500.
Described glass nursery cylinder specification is 40 × 30 × 20cm.
Nylon mesh screen in described step C4 is 80 order nylon mesh screens.
In described step C5, water body pH is 6, and water temperature remains between 20 DEG C, and dissolved oxygen amount is 8.5 mg/litre.
The leech seedling that described raising method is raised is eurysome golden thread leech.
Embodiment 2
The raising method of leech seedling of the present invention, comprises the following steps:
(A) incubator box is prepared:
Select the uncovered casing of plastics or wooden materials, place that one deck 2.5cm is thick and humidity is the rough sand of 35% at bottom half, be that the non-woven fabrics matrix of 2cm is laid on above-mentioned rough sand layer by thickness, soil humidity is 35%; The thickness that matrix is laid bottom surface and wall is the latticed water suction moisturizing layer of 1cm, the sizing grid of water suction moisturizing layer puts into one piece of Whitmania pigra Whitman spawns for suitable, moisturizing layer humidity 35%, incubator box top is covered with black fabric, in casing, the humidity of air remains on 80%, and wall box is hung with the Hygrothermograph for monitoring case environment at any time;
(B) hatching of seedling:
B1, selective body focus on the physically fit adult kind leech of 20g/ bar, put into glass nursery cylinder and lay eggs after sterilization, to control in nursery cylinder water temperature at 20 DEG C, and leech mating also pierces side in cylinder and to loosen the soil middle product cocoon;
B2, leech lay eggs and start to artificially collect for latter 10 days, and get the grid that the ovum cocoon given birth in 3 days is placed in above-mentioned casing, temperature is set to 22 DEG C, treats young leech cocoon-break;
B3, hatch out through 8 days leech seedling;
(C) the raising and train of leech seedling
C1, wide-aperture for 300 × 250 × 30mm specification sponge to be immersed in liquid feed 1 hour, to allow sponge fully receive liquid feed;
C2, by the leech seedling just having hatched out according to constant density be put into bottom be placed with in the glass nursery cylinder of sponge, and above nursery cylinder, hide gauze so as not to leech seedling escape;
C3, every day in the morning 7 and allow leech seedling suck each 2 hours at 11 in evening on sponge respectively, and adopt water spray means to keep nursery cylinder humidity;
C4, each leech suck the stimulation of rear use 30% salt solution and are all brushed down from sponge upper punch by leech, then fish for out by leech seedling with nylon mesh screen immediately, discard salt solution, and cleaned by sponge for subsequent use;
B5, the leech pulled out move to respectively and are equipped with in the nursery cylinder of depth of water 15cm in addition, and the top of nursery cylinder is provided with anti-escape net, and cobble is put into as base in bottom, every morning 10 and 8 oxygenations in evening 2 times, each 0.5 hour;
C6, repeat the feeding process of C3-C5 every day, change the liquid feed in sponge every day.
In described step (A), the light transmittance of the black fabric of hatching initial stage employing is 15%, and the light transmittance of the black fabric of hatching employing in mid-term is 25%, and the light transmittance of the black fabric that the hatching later stage adopts is 45%.
The grid of water suction moisturizing layer is square, rhombus or circle in described step (A).
In described step (C), liquid feed is made up of 250ml cow's serum, 45g raw egg, 40g glucose, 20g dregs of beans, 3g calcium carbonate, 0.03%w/v B B-complex, 10g blood meal, 6g spiral shell meat, 5g saccharomycete and distilled water 500ml.
The preparation method of described liquid feed is: 250ml cow's serum, 45g raw egg, 40g glucose, 20g dregs of beans, 3g calcium carbonate, 0.03%w/v B B-complex, 10g blood meal, 6g spiral shell meat and 5g saccharomycete are fully mixed rear adding distil water to 500ml, and adjust pH is 7.0.
In described step C2, the density of leech seedling is 1500.
Described glass nursery cylinder specification is 40 × 30 × 20cm.
Nylon mesh screen in described step C4 is 80 order nylon mesh screens.
In described step C5, water body pH is 6.5, and water temperature remains between 25 DEG C, and dissolved oxygen amount is 9 mg/litre.
The leech seedling that described raising method is raised is eurysome golden thread leech.
Embodiment 3
The raising method of leech seedling of the present invention, comprises the following steps:
(A) incubator box is prepared:
Select the uncovered casing of plastics or wooden materials, place that one deck 3cm is thick and humidity is the rough sand of 40% at bottom half, be that the non-woven fabrics matrix of 2.5cm is laid on above-mentioned rough sand layer by thickness, soil humidity is 40%; The thickness that matrix is laid bottom surface and wall is the latticed water suction moisturizing layer of 1.5cm, the sizing grid of water suction moisturizing layer puts into one piece of Whitmania pigra Whitman spawns for suitable, moisturizing layer humidity 40%, incubator box top is covered with black fabric, in casing, the humidity of air remains on 85%, and wall box is hung with the Hygrothermograph for monitoring case environment at any time;
(B) hatching of seedling:
B1, selective body focus on the physically fit adult kind leech of 22g/ bar, put into glass nursery cylinder and lay eggs after sterilization, to control in nursery cylinder water temperature at 20 DEG C, and leech mating also pierces side in cylinder and to loosen the soil middle product cocoon;
B2, leech lay eggs and start to artificially collect for latter 11 days, and get the grid that the ovum cocoon given birth in 3 days is placed in above-mentioned casing, temperature is set to 20-25 DEG C, treats young leech cocoon-break;
B3, hatch out through 10 days leech seedling;
(C) the raising and train of leech seedling
C1, wide-aperture for 300 × 250 × 30mm specification sponge to be immersed in liquid feed 1 hour, to allow sponge fully receive liquid feed;
C2, by the leech seedling just having hatched out according to constant density be put into bottom be placed with in the glass nursery cylinder of sponge, and above nursery cylinder, hide gauze so as not to leech seedling escape;
C3, every day in the morning 7 and allow leech seedling suck each 2 hours at 11 in evening on sponge respectively, and adopt water spray means to keep nursery cylinder humidity;
C4, each leech suck the stimulation of rear use 30% salt solution and are all brushed down from sponge upper punch by leech, then fish for out by leech seedling with nylon mesh screen immediately, discard salt solution, and cleaned by sponge for subsequent use;
B5, the leech pulled out move to respectively and are equipped with in the nursery cylinder of depth of water 15cm in addition, and the top of nursery cylinder is provided with anti-escape net, and cobble is put into as base in bottom, every morning 10 and 8 oxygenations in evening 2 times, each 0.5 hour;
C6, repeat the feeding process of C3-C5 every day, change the liquid feed in sponge every day.
In described step (A), the light transmittance of the black fabric of hatching initial stage employing is 20%, and the light transmittance of the black fabric of hatching employing in mid-term is 30%, and the light transmittance of the black fabric that the hatching later stage adopts is 60%.
The grid of water suction moisturizing layer is square, rhombus or circle in described step (A).
In described step (C), liquid feed is made up of 300ml cow's serum, 60g raw egg, 50g glucose, 25g dregs of beans, 5g calcium carbonate, 0.05%w/v B B-complex, 15g blood meal, 7g spiral shell meat, 7g saccharomycete and distilled water 500ml.
The preparation method of described liquid feed is: 300ml cow's serum, 60g raw egg, 50g glucose, 25g dregs of beans, 5g calcium carbonate, 0.05%w/v B B-complex, 15g blood meal, 7g spiral shell meat and 7g saccharomycete are fully mixed rear adding distil water to 500ml, and adjust pH is 7.0.
In described step C2, the density of leech seedling is 1500.
Described glass nursery cylinder specification is 40 × 30 × 20cm.
Nylon mesh screen in described step C4 is 80 order nylon mesh screens.
In described step C5, water body pH is 7, and water temperature remains between 30 DEG C, and dissolved oxygen amount is 10 mg/litre.
The leech seedling that described raising method is raised is eurysome golden thread leech.
Claims (10)
1. a raising method for leech seedling, is characterized in that comprising the following steps:
(A) incubator box is prepared:
Select the uncovered casing of plastics or wooden materials, place that one deck 2-3cm is thick and humidity is the rough sand of 30-40% at bottom half, be that the non-woven fabrics matrix of 1.5-2.5cm is laid on above-mentioned rough sand layer by thickness, soil humidity is 30-40%; The thickness that matrix is laid bottom surface and wall is the latticed water suction moisturizing layer of 0.5-1.5cm, the sizing grid of water suction moisturizing layer puts into one piece of Whitmania pigra Whitman spawns for suitable, moisturizing layer humidity 30-40%, incubator box top is covered with black fabric, in casing, the humidity of air remains on 75-85%, and wall box is hung with the Hygrothermograph for monitoring case environment at any time;
(B) hatching of seedling:
B1, selective body focus on the physically fit adult kind leech of 18-22g/ bar, put into glass nursery cylinder and lay eggs after sterilization, to control in nursery cylinder water temperature at 20 DEG C, and leech mating also pierces side in cylinder and to loosen the soil middle product cocoon;
After B2, leech lay eggs 9-11 days start to artificially collect, and get the grid that the ovum cocoon given birth in 3 days is placed in above-mentioned casing, temperature is set to 20-25 DEG C, treats young leech cocoon-break;
B3, hatch out through 5-10 days leech seedling;
(C) the raising and train of leech seedling
C1, wide-aperture for 300 × 250 × 30mm specification sponge to be immersed in liquid feed 1 hour, to allow sponge fully receive liquid feed;
C2, by the leech seedling just having hatched out according to constant density be put into bottom be placed with in the glass nursery cylinder of sponge, and above nursery cylinder, hide gauze so as not to leech seedling escape;
C3, every day in the morning 7 and allow leech seedling suck each 2 hours at 11 in evening on sponge respectively, and adopt water spray means to keep nursery cylinder humidity;
C4, each leech suck the stimulation of rear use 30% salt solution and are all brushed down from sponge upper punch by leech, then fish for out by leech seedling with nylon mesh screen immediately, discard salt solution, and cleaned by sponge for subsequent use;
B5, the leech pulled out move to respectively and are equipped with in the nursery cylinder of depth of water 15cm in addition, and the top of nursery cylinder is provided with anti-escape net, and cobble is put into as base in bottom, every morning 10 and 8 oxygenations in evening 2 times, each 0.5 hour;
C6, repeat the feeding process of C3-C5 every day, change the liquid feed in sponge every day.
2. according to the raising method of the leech seedling described in claim 1, it is characterized in that: in described step (A), the light transmittance of the black fabric of hatching initial stage employing is 10-20%, the light transmittance of the black fabric of hatching employing in mid-term is 20-30%, and the light transmittance of the black fabric that the hatching later stage adopts is 30-60%.
3. according to the raising method of the leech seedling described in claim 1, it is characterized in that: the grid of water suction moisturizing layer is square, rhombus or circle in described step (A).
4. according to the raising method of the leech seedling described in claim 1, it is characterized in that: in described step (C), liquid feed is made up of 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal, 5-7g spiral shell meat, 3-7g saccharomycete and distilled water 500ml.
5. the raising method of leech seedling according to claim 4, it is characterized in that, the preparation method of described liquid feed is: 200-300ml cow's serum, 30-60g raw egg, 30-50g glucose, 10-25g dregs of beans, 1-5g calcium carbonate, 0.01-0.05%w/v B B-complex, 5-15g blood meal, 5-7g spiral shell meat and 3-7g saccharomycete are fully mixed rear adding distil water to 500ml, and adjust pH is 7.0.
6. the raising method of leech seedling according to claim 1, is characterized in that: in described step C2, the density of leech seedling is 1500.
7. the raising method of leech seedling according to claim 1, is characterized in that: described glass nursery cylinder specification is 40 × 30 × 20cm.
8. the raising method of leech seedling according to claim 1, is characterized in that: the nylon mesh screen in described step C4 is 80 order nylon mesh screens.
9. the raising method of leech seedling according to claim 1, is characterized in that: in described step C5, water body pH is between 6 ~ 7, water temperature remains between 20-30 DEG C, and dissolved oxygen amount is greater than 8 mg/litre.
10. the raising method of leech seedling according to claim 1, is characterized in that: the leech seedling that described raising method is raised is eurysome golden thread leech.
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| CN105900930A (en) * | 2016-04-23 | 2016-08-31 | 贵州畲苗百草民族医药科技开发有限公司 | Early-stage feeding method of young leeches |
| CN106719177A (en) * | 2016-12-14 | 2017-05-31 | 五河县茂源水蛭生态养殖专业合作社 | A kind of cultural method for improving leech seedling percent |
| CN107372237A (en) * | 2017-09-01 | 2017-11-24 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis solid propagation method |
| CN112493186A (en) * | 2020-11-13 | 2021-03-16 | 重庆市药物种植研究所 | Culture method for rapidly increasing anticoagulant enzyme activity of hirudo nipponica |
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| CN105900930A (en) * | 2016-04-23 | 2016-08-31 | 贵州畲苗百草民族医药科技开发有限公司 | Early-stage feeding method of young leeches |
| CN105900930B (en) * | 2016-04-23 | 2019-04-02 | 贵州畲苗百草民族医药科技开发有限公司 | A kind of method for breeding of leech seedling early stage |
| CN106719177A (en) * | 2016-12-14 | 2017-05-31 | 五河县茂源水蛭生态养殖专业合作社 | A kind of cultural method for improving leech seedling percent |
| CN107372237A (en) * | 2017-09-01 | 2017-11-24 | 荆州市民康生物科技有限公司 | A kind of hirudinaria manillensis solid propagation method |
| CN112493186A (en) * | 2020-11-13 | 2021-03-16 | 重庆市药物种植研究所 | Culture method for rapidly increasing anticoagulant enzyme activity of hirudo nipponica |
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