CN105707017B - A kind of eurysome golden thread leech oviposition substrate and preparation method thereof - Google Patents
A kind of eurysome golden thread leech oviposition substrate and preparation method thereof Download PDFInfo
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- CN105707017B CN105707017B CN201610148852.4A CN201610148852A CN105707017B CN 105707017 B CN105707017 B CN 105707017B CN 201610148852 A CN201610148852 A CN 201610148852A CN 105707017 B CN105707017 B CN 105707017B
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- eurysome golden
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a kind of eurysome golden thread leech oviposition substrate, including volumes below than raw material:25 30 parts of soil base-material, 15 30 parts of fish pond bed mud, 5 10 parts of straw powder, 10 20 parts of cripple, eel grass and duckweed starch 0.5 1 parts, 5 10 parts of sand.Its production method comprises the following steps：（1）The working process of raw material,（2) mixed fermentation:Soil base-material, fish pond bed mud base-material, cripple, straw powder and withered grass and duckweed slurry are uniformly mixed into mixed resin, mixed resin is then piled into round heap, is completely covered with the mud of mixing straw and carries out fermentation maturity processing,（3) measure, adjust physical and chemical index.Matrix production method produced by the invention is simple, workable, of low cost, easy to use, can be widely applied to eurysome golden thread leech oviposition breeding.
Description
Technical field
The present invention relates to a kind of eurysome golden thread leech oviposition substrates and preparation method thereof, belong to artificial culture technology field.
Background technology
Eurysome golden thread leech（Whitmania pigra Whitman）Long 6cm~the 15cm of body, the wide 1.1cm~2.4cm of body are in
Spindle, the back side are dirty-green, the longitudinal grin for having 5 black Huangs alternate, and 107 section of body point, 5 pairs of eyespots are front and rear respectively to have a sucker, are subordinate to
Annelida (Annelida) Hirudinea (Hirudinea), it is a kind of common medicinal aquatic livestock.Its internal institute's hirudin energy
Inhibit the formation of thrombus and the division of tumour cell, be the main component for treating cardiovascular and cerebrovascular diseases medicament.In recent years, with it
The exploitation of medical value, the market demand increasingly increase, and increasingly the wild resource of failure cannot meet the needs, so numerous breeding prospect
Well, and key that eurysome golden thread leech breeding is grown is that possess good egg-laying environment.
As oviposition substrate it is one of major way of current eurysome golden thread leech breeding using nature soil property.Oviposition substrate
Composition is general mostly to be rich in based on the fertile mud of humus, animal wastes, weeds etc..But this matrix is there are gas porosity and thoroughly
The shortcomings of gas is poor, easily hardened；The deficiencies of animal wastes are although full of nutrition, but few there are quantity, and hardly possible is collected, it is impossible to meet work
The needs of factory's metaplasia production.Therefore find a kind of cheap, good permeability, gas porosity it is high burrow convenient for eurysome golden thread leech, do cocoon,
The matrix of oviposition is to solve the key link of eurysome golden thread leech low reproduction rate.
The content of the invention
The purpose of the present invention is being directed to the deficiency of existing eurysome golden thread leech oviposition substrate, provide a kind of production method it is simple,
Workable, of low cost, spawning rate can improve 10%, the oviposition substrate of 3 years can be used continuously.
The technical scheme is that be accomplished by the following way:
A kind of eurysome golden thread leech oviposition substrate, including volumes below than raw material:25-30 parts of soil base-material, fish pond bed mud
15-30 parts, 5-10 parts or 15 parts of straw powder, 10-20 parts of cripple, eel grass and duckweed starch 0.5-1 parts or 5 parts, 5-10 parts of sand.
The soil base-material is the agricultural land soils such as rice field or the wheatland of clean pollution-free non agricultural chemical residuum, and particle is uniformly without plate
Knot.
The fish pond bed mud is the pond sludge year in year out in the 1-15cm of pool bottom.
The straw powder be the material of rice or the cereal crops such as wheat or corn stalk after crushed, grain size≤2cm.
The cripple be material of the methane-generating pit bottom sediment after drying crushing and processing, granularity 1-3mm.
The eel grass and duckweed slurry are fresh eel grass and duckweed in mass ratio 1:By smashing the thick of acquisition to pieces after the mixing of 1 ratio
Slurries.
The sand is clean free of contamination river sand, grain size 0.35-0.5mm.
The matrix be water content 30-40%, total porosity 55-75%, pH value 6.0-7.5 slant acidities, the content of organic matter >=25%
Single-size.
The preferential formula volume ratio of the matrix is:30 parts of soil base-material, 30 parts of fish pond bed mud, 15 parts of straw powder, cripple 10
Part, eel grass and duckweed starch 5 parts, 10 parts of sand.
The preferred physical and chemical index of the matrix is:Water content 35%, total porosity 60%, the content of organic matter 40%, PH6.5.
A kind of production method of eurysome golden thread leech oviposition substrate, it is characterised in that:
（1）The working process of raw material
1. the cleaning of soil base-material is crushed uniformly to no weeds, rubble and caking, it is exposed to the sun and is less than 20% to moisture;
2. fish pond bed mud removes bulk impurity, stand is even to be exposed to the sun to moisture less than 20%, crushes uniform;
3. handling cripple water content to less than 20%, pulverize and sieve to 10 mesh;
4. stalk crushing is screened to grain size≤2cm;
5. fresh eel grass and duckweed press 1:Smash the thick slurries of acquisition after the mixing of 1 ratio to pieces with mixer；
6. clean free of contamination river sand is screened to grain size 0.35-0.5mm.
（2) mixed fermentation:By step 1.-the soil base-material, fish pond bed mud base-material, cripple, straw powder, withered that 6. obtains of step
Grass and duckweed slurry, river sand are uniformly mixed into mixed resin, and mixed resin then is piled round heap, are covered completely with the mud of mixing straw
Lid carries out fermentation maturity processing.
（3) measure, adjust physical and chemical index:Meet water content, total porosity, pH indexs：Water content 30-40%, total pore space
Spend 55-75%, pH value 6.0-7.5 slant acidities, the single-size of the content of organic matter >=25%.
The mixed resin fermentation maturity processing time is 20-40 days；
The mud of the mixing straw refers to for straw to be cut into section not of uniform size, uniform mud mixing.
Beneficial effects of the present invention:
1st, fish pond bed mud, straw powder, eel grass and duckweed slurry are all organic wastes, and quantity is more, and purposes is few, dynamic with its replacement
The raw materials such as object excrement, for making eurysome golden thread leech oviposition substrate, it is possible to reduce environmental pollution is turned waste into wealth, and is turned harm into good.
2nd, the present invention makes the coarse size river sand of any and fine size soil, fish pond bed mud, the cripple mix and match of some
The existing retentiveness of matrix has gas permeability again, and gas porosity is high, avoids easily hardened, poor air permeability the limitation of various regions different soils.
Meanwhile the acid-base value of matrix can be adjusted using the higher straw powder of alkalescence.Withered grass and duckweed slurry can resist containing abundant nutrition
Bacterium desinsection creates clean egg-laying environment for eurysome golden thread leech.
3rd, matrix production method produced by the invention is simple, workable, of low cost, easy to use, can be extensive
It lays eggs and breeds applied to eurysome golden thread leech.
Specific embodiment
Further comparative descriptions are made to the use of the present invention with reference to example.
1. material
1) eurysome golden thread leech
Weight is 25 ± 1g, has been fertilized, the healthy and strong eurysome golden thread leech kind leech without wound
2160.
2) spawning pond
Specification：Cement pit in 12 length × width × height=4m × 1.5m × 0.6m rooms installs anti-escape network, 18 plastics respectively
Basket bait table（Length × width × height=1.5m × 0.6m × 0.1m, every basket puts fresh and alive spiral shell 3kg.
3) oviposition substrate
1. matrix 1
Raw material and Formulation Component Weight ratio are：100 parts of soil base-material, it is 6.5 to adjust pH value.
2. matrix 2
Raw material and Formulation Component Weight ratio：40 parts of pool base fertilizer mud, 10 parts of pig manure, 10 parts of cow dung, 10 parts of chicken manure, green grass 30
Part, mixed fermentation, it is 6.5 to adjust pH value.
3. matrix 3
Raw material and Formulation Component Weight ratio：30 parts of soil base-material, 30 parts of fish pond bed mud, 15 parts of straw powder, 10 parts of cripple are bitter
Grass and duckweed starch 5 parts, and 10 parts of sand, it is 6.5 to adjust pH value.
4) instrument
Electronic balance（Shanghai Precision Scientific Apparatus Co., Ltd produces）, moisture teller（Zhong Tian companies produce）, air-conditioning
（Haier）.
2. method
1）District's groups
This experiment uses oviposition substrate as main process task, water content 40%, and 3 repetitions of each matrix each repeat to launch at random
240 eurysome golden thread leech kind leech, while each matrix sets a blank control for measuring moisture at any time, convenient for supplementing water at any time
Point.
2）Experimental design
Each cement pit bottom surrounding leans on the wall thorough 40cm wide of matrix homogeneous reactor, the ridge of 20cm high, and 40cm wide is covered on the ridge
Straw mat pays attention to observing the situation that burrows after launching kind of leech.Experiment carries out in identical environment：Keep constant 25 DEG C of temperature, matrix contains
Water 40%, spiral shell Empty grain rate≤50%.
3）Statistical analysis
It digs after starting experiment 15 days and takes ovum statistics laying, the death rate as shown in table 1.
Influence of 1 different substrates of table to eurysome golden thread leech laying and the death rate
As can be seen from Table 1, under same humidity, temperature conditionss, leech laying in matrix 3 is most, the death rate is minimum,
Laying improves 11.1% and 16.9% than matrix 1, matrix 2 respectively, death rate difference low 8.2% and 13.57%.
Claims (9)
1. a kind of preparation method of eurysome golden thread leech oviposition substrate, it is characterised in that：The matrix include volumes below than original
Material：25-30 parts of soil base-material, bed mud 15-30 parts of fish pond, 15 parts of straw powder, 10-20 parts of cripple, eel grass and duckweed starch 5 parts, sand
It is 5-10 parts sub-；The production method of the eurysome golden thread leech oviposition substrate, comprises the following steps：
（1）The working process of raw material
1. the cleaning of soil base-material is crushed uniformly to no weeds, rubble and caking, it is exposed to the sun and is less than 20% to moisture；
2. fish pond bed mud removes bulk impurity, stand is even to be exposed to the sun to moisture less than 20%, crushes uniform；
3. handling cripple water content to less than 20%, pulverize and sieve to 10 mesh；
4. stalk crushing is screened to grain size≤2cm；
5. fresh eel grass and duckweed press 1:Smash the thick slurries of acquisition after the mixing of 1 ratio to pieces with mixer；
6. clean free of contamination sand is screened to grain size 0.35-0.5mm；
（2) mixed fermentation:By step 1.-step the soil base-material, fish pond bed mud, cripple, straw powder, withered grass and the duckweed that 6. obtain
Slurry, sand are uniformly mixed into mixed resin, and mixed resin then is piled round heap, are completely covered and are sent out with the mud of mixing straw
The decomposed processing of ferment；
（3) measure, adjust physical and chemical index:Meet water content, total porosity, pH indexs：Water content 30-40%, total porosity
55-75%, pH value 6.0-7.5 slant acidities, the single-size of the content of organic matter >=25%.
2. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The soil base
Expect the rice field for clean pollution-free non agricultural chemical residuum or Wheat Growing Soils, particle is uniformly without hardened.
3. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The fish pond bottom
Mud is the pond sludge year in year out in the 1-15cm of pool bottom.
4. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The straw powder
For the material of rice or wheat or corn crop stalk after crushed, grain size≤2cm.
5. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The cripple is
Material of the methane-generating pit bottom sediment after drying crushing and processing, granularity 1-3mm.
6. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The eel grass and
Duckweed slurry presses 1 for fresh eel grass and duckweed:By smashing the thick slurries of acquisition to pieces after the mixing of 1 ratio.
7. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The sand is
Clean free of contamination river sand, grain size 0.35-0.5mm.
8. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The matrix formulations
Volume ratio is：30 parts of soil base-material, 30 parts of fish pond bed mud, 15 parts of straw powder, 10 parts of cripple, eel grass and duckweed starch 5 parts, sand
10 parts.
9. a kind of preparation method of eurysome golden thread leech oviposition substrate as described in claim 1, it is characterised in that：The mixed base
It is 20-40 days to expect fermentation maturity processing time.
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| CN105707017B true CN105707017B (en) | 2018-06-01 |
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| CN106688987B (en) * | 2016-12-16 | 2019-12-10 | 广西壮族自治区药用植物园 | Method for adjusting pH value of soil of leech spawning platform and eliminating soil putrid odor |
| CN114946775A (en) * | 2022-06-21 | 2022-08-30 | 楚雄师范学院 | Production bed formula soil for poecilobdella manillensis egg cocoon incubation, preparation method and use method thereof |
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| CN101766154A (en) * | 2010-01-20 | 2010-07-07 | 龚元 | Method for high-density green ecological culture of blood-sucking leeches |
| CN101849530A (en) * | 2010-06-23 | 2010-10-06 | 南京农业大学 | Substrate for hatching Whitmania pigra Whitman spawns |
| CN102630649A (en) * | 2012-05-16 | 2012-08-15 | 句容市润泰生物科技有限公司 | Leech fry raising method |
| CN103283686A (en) * | 2013-06-17 | 2013-09-11 | 抚州市水产科学研究所 | Leech soilless breeding method |
| CN103493786A (en) * | 2013-09-10 | 2014-01-08 | 江苏农林职业技术学院 | Method for improving leech larva culturing |
| CN104904674A (en) * | 2015-06-19 | 2015-09-16 | 广西复鑫益生物科技有限公司平南分公司 | Breeding method for young leeches |
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2016
- 2016-03-16 CN CN201610148852.4A patent/CN105707017B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766154A (en) * | 2010-01-20 | 2010-07-07 | 龚元 | Method for high-density green ecological culture of blood-sucking leeches |
| CN101849530A (en) * | 2010-06-23 | 2010-10-06 | 南京农业大学 | Substrate for hatching Whitmania pigra Whitman spawns |
| CN102630649A (en) * | 2012-05-16 | 2012-08-15 | 句容市润泰生物科技有限公司 | Leech fry raising method |
| CN103283686A (en) * | 2013-06-17 | 2013-09-11 | 抚州市水产科学研究所 | Leech soilless breeding method |
| CN103493786A (en) * | 2013-09-10 | 2014-01-08 | 江苏农林职业技术学院 | Method for improving leech larva culturing |
| CN104904674A (en) * | 2015-06-19 | 2015-09-16 | 广西复鑫益生物科技有限公司平南分公司 | Breeding method for young leeches |
Non-Patent Citations (1)
| Title |
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