CN101849530A - Substrate for hatching Whitmania pigra Whitman spawns - Google Patents
Substrate for hatching Whitmania pigra Whitman spawns Download PDFInfo
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- CN101849530A CN101849530A CN 201010206929 CN201010206929A CN101849530A CN 101849530 A CN101849530 A CN 101849530A CN 201010206929 CN201010206929 CN 201010206929 CN 201010206929 A CN201010206929 A CN 201010206929A CN 101849530 A CN101849530 A CN 101849530A
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- hatching
- whitmania pigra
- pigra whitman
- whitman spawns
- matrix
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Abstract
The invention provides a high-efficiency substrate for artificially hatching Whitmania pigra Whitman spawns and aims to provide a substrate for hatching Whitmania pigra Whitman with shorter incubation time, higher hatching rate and more concentrated seedling emergence compared with the conventional hatching technique. By using the substrate with the best moisture holding capacity, the hatching time can be shortened by 7 days compared with the conventional hatching time, the hatching rate is increased by 22.7 percent, and the seedling emergence is concentrated. The substrate not only is beneficial to factory management for hatching the Whitmania pigra Whitman, but also prolongs the feeding time of Whitmania pigra Whitman seedlings, the adaptive capacity of the Whitmania pigra Whitman to the environment and the survival rate are improved through ingestion, growth and energy storage of the Whitmania pigra Whitman seedlings as soon as possible, the per mu yield of the Whitmania pigra Whitman is increased indirectly, and the economic benefit of culturing the Whitmania pigra Whitman is increased. By using the substrate, the per mu yield of the Whitmania pigra Whitman can be increased by about 15kg indirectly; and based on 600 RMB/kg of the current market price, the economic benefit of the Whitmania pigra Whitman per mu is increased by 6,000 to 9,000 RMB.
Description
One, technical field
The present invention relates to a kind of matrix of Whitmania pigra Whitman spawns artificial incubation.
Two, background technology
Leech as herein described refers to leech Whitmania pigra Whitman, also be called eurysome golden thread leech, " one of the main base of the Chinese pharmacopoeia medicinal material leech of including is former to be 2010 editions, has broken blood, by becoming silted up, effect such as stimulate the menstrual flow is a primary raw material of dredging numerous treatment cardiovascular and cerebrovascular disease Chinese patent drugs such as blood is logical, brain blood health.Because the individual big fast growth of leech, along with the further investigation to aspects such as its physiological properties, it is propagated artificially and has obtained initial success, has slowed down the contradiction between the market demand and the leech medicinal material quantity delivered in recent years.
Leech artificial propagation techniques from present grasp, the leech brooding time is about one month, the seedling that hatches must be cultured the pond seedling and bring up about 1 month, just can go into commodity to leech seedling by the end of June and culture the pond and culture, and can gather successively to mid or late November.Therefore the length of brooding time is related to the time that the leech seedling enters the commodity pond, has determined the growth time of leech, thereby finally can influence the output of leech.
Three, summary of the invention
The objective of the invention is on the basis of existing hatching technique, provide a kind of and shorten 7 days than existing brooding time, incubation rate improves 22.7%, the leech hatching matrix of emerging concentrated.
The selected matrix of the present invention is that water-holding capacity is 100%～120% fine perlite.This fine perlite is the cellular white particle in a kind of very light inside.Have nontoxic, tasteless, do not fire, characteristics such as without putrefaction, be insoluble to strong acid-base, be slightly soluble in hydrofluoric acid, the pH value is neutral (6.5～7.5).The main chemical compositions of this fine perlite and percentage by weight thereof are SiO
270～75%, CaO 0.1～2.0%, Al
2O
312～16%, Na
2O 1.0～5.0%, Fe
2O
30.1 K～1.5%,
2O1.0～5.0%.
According to the present invention and in conjunction with existing hatching technique, also invented a kind of hatching Whitmania pigra Whitman spawns method of utilizing matrix of the present invention: shop one layer thickness is that the water-holding capacity of 2.5cm is 100%～120% fine perlite bottom incubator, at fine perlite upper berth one deck wet gauze, on gauze, the Whitmania pigra Whitman spawns of gathering is successively put well.Again at ovum cocoon upper berth one deck wet gauze, the water-holding capacity of putting a layer thickness and be 3cm on gauze is 100%～120% fine perlite, covers the incubator lid then, and putting into temperature is 25～28 ℃, and relative moisture is to hatch in 75%～80% the artificial incubation case.
Statistics and relatively finding is compared with the thin wet soil that uses in the existing hatching technique by experiment, the utilization water-holding capacity be 100%～120% fine perlite as hatching Whitmania pigra Whitman spawns matrix, its seedling-growing time is more concentrated, speed of emergence is fast, output of seedling is big.So not only help the factory management of leech hatching, more increased the time that the leech seedling conforms and raises, make the leech seedling, finally strengthen survival rate by growth and the storage power of ingesting.Improve the per mu yield of leech indirectly by the present invention, increased the economic benefit of hirudiniculture.Can improve the about 15kg of leech per mu yield indirectly according to incompletely statistics, calculate by 600 yuan/kg of present leech medicinal material market price, every mu increases about 6000～9000 yuan of economic benefit approximately.
Four, embodiment
1. material
1.1 experiment material: ovum cocoon (provided by traditional Chinese medicine research institute of Agricultural University Of Nanjing leech base, its parent is accredited as the ripe body of Whitmania pigra Whitman through professor Guo Qiaosheng), fine earth earth, fine perlite, hospital gauze.
1.2 experimental instrument: artificial climate incubator (Zhejiang Top instrument), balance (going up Nereid section balance), and cutting ring, 20*15*30cm crisper;
2 methods
2.1 determining of maximum water-holding capacity
Maximum water-holding capacity: 100cm
3Cutting ring will get and put into basin after required soil and perlite are added a cover respectively, soaked about 24 hours.The water surface will be lower than cutting ring 2～4mm suitable for reading, does not make cutting ring water inlet suitable for reading, gets identical soil (perlite) simultaneously, and air-dry back is by No. 18 sieves (1mm), packs in the cutting ring that ties with the individual layer bundle, makes real gently after installing.To fill wet soil then and open, this cutting ring will be placed on the cutting ring that fills air-dry soil (perlite) together with filter paper with wet perlitic cutting ring bottom.Simultaneously on two cutting rings, press weight, make it tight contact.After 8 hours, original state wet soil (perlite) 10～20g that gets the upper strata cutting ring puts into the aluminium box and weighs immediately, dries, weighs, and carries out repeatedly 2～3 times, gets its mean value, is soil and perlitic maximum water-holding capacity.
Maximum water-holding capacity %=100 * (wet soil weight-oven-dried soil is heavy)/oven-dried soil is heavy
2.2 grouping
This experiment adopts the master to be treated to culture matrix (2 levels of soil and perlite), and vice processing is the split plot district group experimental design of culture matrix humidity (5 levels), and totally 10 processed group are put 30 pieces of Whitmania pigra Whitman spawns for every group at random, and every processing repeats for 3 times.According to preliminary experiment, the design of culture matrix humidity, soil constitution is not 40%, 60%, 80%, 100%, 120% (abbreviating soil 40%, soil 60%, soil 80%, soil 100%, soil 120% as) of maximum water-holding capacity, and the perlite group is respectively 60%, 80%, 100%, 120%, 140% (abbreviating precious 60%, precious 80%, precious 100%, precious 120%, precious 140% as) of maximum water-holding capacity.
Simultaneously soil group and perlite group select again respectively maximum water-holding capacity be 100% one group as the blank group, be convenient to determine replenish the moisture that scatters and disappears every day, to guarantee the constant of experiment condition.
2.3 experimental design
In the matrix of crisper bottom shop one bed thickness 2.5cm,, on gauze, the Whitmania pigra Whitman spawns of gathering is successively put well at matrix upper berth one deck wet gauze.At ovum cocoon upper berth one deck wet gauze, the matrix of putting a bed thickness 3cm on gauze covers the crisper lid then again, and putting into temperature is 25～28 ℃, and relative moisture is to hatch in 75%～80% the artificial incubation case.
For preventing that in experimentation the evaporation of moisture in the box causes the variation of water-holding capacity, under identical experimental situation, measure the reduction of blank group moisture by gravitational method and determine a day amount of water, replenish every group of moisture evaporated, to keep the required soil humidity of experiment.
At experimental session, weigh every day and the additional moisture that scatters and disappears, whether have ovum cocoon begin hatching, choose the leech of hatching every day and write down every group of leech number that hatches if observing simultaneously.
2.4 statistical method
Test data is earlier done preliminary treatment with Microsoft Excel, adopt then SPSS 13.0 software kits (OnewayA NOVA LSD) analyzes, data all with
Expression.
3 results and analysis
3.1 different humidity is to the trend that influences of hatching Whitmania pigra Whitman spawns
Different humidity has apparent in view variation tendency to the influence of Whitmania pigra Whitman spawns as can be seen from Table 1.Soil from maximum water-holding capacity 40%～120% between, Whitmania pigra Whitman spawns has one to arrive low process from low to high again, and is wherein the highest at 80% place.Perlite from maximum water-holding capacity 60%～140% between, Whitmania pigra Whitman spawns has one to arrive low process from low to high again, and is wherein the highest between 100%～120%.
Table 1 different humidity matrix is to the influence of leech hatching
3.2 different substrates is to the influence of hatching Whitmania pigra Whitman spawns
Under the same humidity condition, be that matrix is more than the hatching Whitmania pigra Whitman spawns number that is matrix with soil as can be seen from Table 1 with the perlite.ANOVA showed significant does not have the significant difference between the perlite group of the soil group of 60% perlite group and 80% and 100% and 120%, between all the other each groups significant difference is arranged all.100% perlite group has improved 22.7% than the incubation rate of 80% soil group.
3.3 different substrates is to the influence of hatching Whitmania pigra Whitman spawns speed
The soil group was from the 5th day as can be seen from Table 2, young leech hatching quantity under each damp condition begins to increase, the hatching peak appears at the 10th～11 day substantially, and the hatching individual amount has obviously begun no longer to occur big variation in the time of the 17th day, can think substantially to have hatched until the 20th day and finish.The whole incubation period continues about 20 days.
The perlite group was from the 5th day, young leech hatching quantity under each damp condition sharply increases, and during to 9 days, the hatching peak appears at the 6th～9 day substantially, to the 12nd day time hatching individual amount do not had obvious increase, can think substantially to have hatched until the 13rd day and finish.The whole incubation period continues about 13 days.
The perlite group hatching peak phase is hatched the duration obviously than soil group weak point early than the soil group as can be seen from Table 2, and it is comparatively concentrated to emerge.So not only help the factory management of leech hatching, more increased the time that the leech seedling conforms, make the leech seedling, finally strengthen survival rate, improve output early by the growth storage power of ingesting.
Table 2 different substrates is to the influence of leech hatching speed
Claims (6)
1. the matrix of a hatching Whitmania pigra Whitman spawns, it is characterized in that: the matrix that is used for hatching Whitmania pigra Whitman spawns is the fine perlite of water-holding capacity between 100%～120%.
2. " a kind of matrix of hatching Whitmania pigra Whitman spawns " according to claim 1, it is characterized in that: this fine perlite is the cellular white particle in a kind of very light inside, have nontoxic, tasteless, do not fire, characteristics such as without putrefaction, be insoluble to strong acid-base, be slightly soluble in hydrofluoric acid, the pH value is 6.5～7.5.
3. " a kind of matrix of hatching Whitmania pigra Whitman spawns " according to claim 1, it is characterized in that: the main chemical compositions of this fine perlite and percentage by weight thereof are SiO
270～75%, CaO 0.1～2.0%, Al
2O
312～16%, Na
2O1.0～5.0%, Fe
2O
30.1 K～1.5%,
2O 1.0～5.0%.
4. " a kind of matrix of hatching Whitmania pigra Whitman spawns " according to claim 1, its hatching Whitmania pigra Whitman spawns method is: at shop, incubator bottom one deck hatching matrix fine perlite, at fine perlite upper berth one deck wet gauze, on gauze, the Whitmania pigra Whitman spawns of gathering is successively put well, again at ovum cocoon upper berth one deck wet gauze, on gauze, put one deck fine perlite, cover the incubator lid then, put into temperature and be 25～28 ℃, relative moisture and be 75%～80% artificial incubation case and hatch.
5. hatching Whitmania pigra Whitman spawns method according to claim 4 is characterized in that: the thickness that is used for the incubator bottom fine perlite of hatching Whitmania pigra Whitman spawns is 2.5cm, and the thickness of the superiors' fine perlite is 3cm.
6. hatching Whitmania pigra Whitman spawns method according to claim 4 is characterized in that: the Whitmania pigra Whitman spawns that is used to hatch is the ovum cocoon of eurysome golden thread leech Whitmania pigra Whitman.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102362587A (en) * | 2011-03-30 | 2012-02-29 | 重庆时珍阁普生药业有限公司 | Artificial soilless leech hatching method |
| CN103283686A (en) * | 2013-06-17 | 2013-09-11 | 抚州市水产科学研究所 | Leech soilless breeding method |
| CN103493786A (en) * | 2013-09-10 | 2014-01-08 | 江苏农林职业技术学院 | Method for improving leech larva culturing |
| CN103493787A (en) * | 2013-10-10 | 2014-01-08 | 云南海瑞迪生物药业有限公司 | Method for incubating leech cocoons |
| CN103636564A (en) * | 2013-11-19 | 2014-03-19 | 靖江市明星水蛭养殖专业合作社 | Artificial soilless leech hatching method |
| CN103918619A (en) * | 2014-05-05 | 2014-07-16 | 徐加临 | Leech egg hatching medium |
| CN104509495A (en) * | 2015-01-07 | 2015-04-15 | 衡阳市首龙渔牧发展有限公司 | Snail incubation technology |
| CN105707017A (en) * | 2016-03-16 | 2016-06-29 | 江苏省农业科学院宿迁农科所 | Whitemania pigra whiteman ovipositional substrate and preparation method thereof |
| CN106035240A (en) * | 2016-06-01 | 2016-10-26 | 吴江市明星产业种养殖中心 | Leech incubating method |
| CN114946775A (en) * | 2022-06-21 | 2022-08-30 | 楚雄师范学院 | Production bed formula soil for poecilobdella manillensis egg cocoon incubation, preparation method and use method thereof |
Citations (2)
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| CN101627747A (en) * | 2009-05-08 | 2010-01-20 | 陆如明 | Method for hatching leeche and raising seedling of leeche |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102362587A (en) * | 2011-03-30 | 2012-02-29 | 重庆时珍阁普生药业有限公司 | Artificial soilless leech hatching method |
| CN103283686B (en) * | 2013-06-17 | 2015-02-25 | 抚州市水产科学研究所 | Leech soilless breeding method |
| CN103283686A (en) * | 2013-06-17 | 2013-09-11 | 抚州市水产科学研究所 | Leech soilless breeding method |
| CN103493786A (en) * | 2013-09-10 | 2014-01-08 | 江苏农林职业技术学院 | Method for improving leech larva culturing |
| CN103493786B (en) * | 2013-09-10 | 2015-05-27 | 江苏农林职业技术学院 | Method for improving leech larva culturing |
| CN103493787A (en) * | 2013-10-10 | 2014-01-08 | 云南海瑞迪生物药业有限公司 | Method for incubating leech cocoons |
| CN103636564A (en) * | 2013-11-19 | 2014-03-19 | 靖江市明星水蛭养殖专业合作社 | Artificial soilless leech hatching method |
| CN103918619A (en) * | 2014-05-05 | 2014-07-16 | 徐加临 | Leech egg hatching medium |
| CN104509495A (en) * | 2015-01-07 | 2015-04-15 | 衡阳市首龙渔牧发展有限公司 | Snail incubation technology |
| CN105707017A (en) * | 2016-03-16 | 2016-06-29 | 江苏省农业科学院宿迁农科所 | Whitemania pigra whiteman ovipositional substrate and preparation method thereof |
| CN105707017B (en) * | 2016-03-16 | 2018-06-01 | 江苏省农业科学院宿迁农科所 | A kind of eurysome golden thread leech oviposition substrate and preparation method thereof |
| CN106035240A (en) * | 2016-06-01 | 2016-10-26 | 吴江市明星产业种养殖中心 | Leech incubating method |
| CN114946775A (en) * | 2022-06-21 | 2022-08-30 | 楚雄师范学院 | Production bed formula soil for poecilobdella manillensis egg cocoon incubation, preparation method and use method thereof |
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| CN101849530B (en) | 2011-10-19 |
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