CN102031231A - Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide - Google Patents
Growth-promoting rhizobacteria and fungicide thereof, preparation method and application of fungicide Download PDFInfo
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Abstract
The invention discloses growth-promoting rhizobacteria and fungicide thereof, as well as a preparation method and application of the fungicide, namely Klebsiellapneumoniae and the fungicide taking the Klebsiellapneumoniae used as an active ingredient, the preparation method of the fungicide and application of the fungicide in phosphorus dissolving and nitrogen fixation. The invention has the advantages that the nitrogen fixing and phosphorus dissolving bacterium integrated with multiple growth-promoting capabilities, such as phosphorous dissolving, nitrogen fixing and auxin producing, into a whole is provided to prepare the nitrogen fixing and phosphorous dissolving fungicide, the fungicide has the characteristics of complete growth promotion performance, high fertilizer efficiency and low cost, and is suitable for the regions lacking nitrogen and phosphorous and having hungry soil; and the preparation process is simple and convenient, high fertilizer efficacy of nitrogen fixing and phosphorous dissolving is obtained, and the invention has excellent development prospect.
Description
Technical field
The present invention relates to a kind of plant growth-promoting microbial inoculum, specifically a kind of plant growth-promoting bacteria and microbial inoculum thereof, and its production and application, biological fertilizer field belonged to.
Background technology
Nitrogen and phosphorus are as the essential mineral element of plant, be the important composition composition of multiple 26S Proteasome Structure and Function materials such as plant viable cell internal protein, nucleic acid, phosphatide, enzyme, plant hormone, plant energy metabolism, organism synthetic with metabolism, substance operation, genetic expression and processes such as regulation and control and cell signalling in play an important role.The shortage of nitrogen and phosphorus element, will make growth and development of plant be subjected to press down, cause the decline of crop yield and quality.Use chemical fertilizer for a long time in a large number and not only improved agriculture production cost, and caused the selectivity accumulation of some element in the soil and the deterioration of soil physico-chemical property, environment is polluted.Therefore substituting or partly substitute chemical fertilizer with microbial fertilizer is the inexorable trend of keeping agricultural sustainable development.The soil bacteria that is present in plant rhizosphere can supply plant absorbing to promote the growth of crop by the insoluble phosphorus of depositing in a large number in rhizosphere fixed nitrogen, generation growth hormone, the dissolving soil, reaches the short purpose of promoting production of giving birth to.
Mostly be many bacterial classifications mix bacterium agent owing to have the difficult molten phosphorus nitrogen-fixing plants growth promoting bacteria agent that obtains, therefore puts into production at present of the bacterial classification of multiple efficient growth-promoting functions simultaneously, have antagonism between bacterial classification, problems such as nutritional needs and metabolism imbalance in the microbial inoculum.
Summary of the invention
The objective of the invention is to, at the problems referred to above, the invention provides a kind of with fixed nitrogen phosphorus-solubilizing bacteria that to collect the multiple short living ability of molten phosphorus, fixed nitrogen and product growth hormone be one, be prepared into the agent of fixed nitrogen phosphorus-solubilizing bacteria, having short natural disposition can be comprehensively, and fertilizer efficiency height, cheap characteristics are applicable to that nitrogen phosphorus lacks, there is bright development prospect in the zone that soil is barren.
Technical scheme of the present invention is: Klebsiella pneumonia (Klebsiella pneumoniae), its microbial preservation unit is: China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date on August 31st, 2010, preserving number is: CGMCC No.4128; The morphological feature of described Klebsiella pneumonia is: the straight-bar bacterium, diameter 0.5～0.8 μ m, long 3.2～5.0 μ m, single distribution, pod membrane is arranged, Gram-negative, do not move, be grown on the YMA medium and to produce a large amount of polysaccharide of circular translucent colony justacrine, the effect of fixed nitrogen, dissolving inorganic phosphorus is arranged, can glucose, sucrose, Zulkovsky starch and N.F,USP MANNITOL is sole carbon source.
Klebsiella pneumonia (
Klebsiella pneumoniae), microbial preservation number is: CGMCC No. 4128, and as the application in the plant growth-promoting microbial inoculum.
A kind of preparation method of plant growth-promoting microbial inoculum, in carrying out according to the following step:
(1) Klebsiella pneumonia (
Klebsiella pneumoniae) RSN19 is inoculated in the YMA solid medium, at 26 ℃～28 ℃ activation 18～24h;
(2) activation after
Klebsiella pneumoniaeRSN19 is inoculated in and carries out fermentation culture in the liquid nutrient medium, and temperature is 25 ℃～29 ℃, is cultured in every milliliter of fermented liquid
Klebsiella pneumoniaeRSN19 bacterium number is 4～6 10
10Individual;
(3) with in the above-mentioned steps (2)
Klebsiella pneumoniaeThe zymocyte liquid of RSN19, aseptic liquid nutrient medium and concentration are that 10% pH 7.0 aseptic phosphoric acid buffers are 4～8: 2～4 according to volume ratio: 1 mixed; With aseptic aluminium foil bag or the encapsulation of aseptic Polypropylene Bag, make single bacterial classification liquid bacterial agent;
(4) will state the liquid bacterial agent of preparation in the step (3) and aseptic peat dust is 1: 4～8 mixed according to volume ratio, makes solid microbial.
The liquid nutrient medium of described growth promoting bacteria agent consists of, according to the KH of weight fraction ratio by 0.5～1 part
2PO
4, 1～2 part yeast powder, 5～10 parts glucose, 0.2～0.4 part MgSO
4, 0.05～0.1 part NaCl, 900～1000 parts water is formed, and wherein the pH value of liquid nutrient medium is 6.4～7.4.
Zymocyte liquid, aseptic liquid nutrient medium and aseptic phosphoric acid buffer are 6: 3: 1 mixed according to volume ratio in the described step (3).
Liquid bacterial agent and aseptic peat dust volume ratio are 1: 6 in the described step (4).
Follow a kind of plant growth-promoting microbial inoculum that is prepared from according to above-mentioned preparation method, the application in the molten phosphorus of fixed nitrogen.
The invention has the advantages that: provide a kind of with fixed nitrogen phosphorus-solubilizing bacteria that to collect the multiple short living ability of molten phosphorus, fixed nitrogen and product growth hormone be one, be prepared into the agent of fixed nitrogen phosphorus-solubilizing bacteria, have short natural disposition can be comprehensively, fertilizer efficiency height, cheap characteristics, be applicable to that nitrogen phosphorus lacks, the zone that soil is barren, preparation technology is easy, the molten phosphate fertilizer of fixed nitrogen is imitated high, and bright development prospect is arranged.
Microbial inoculum of the present invention is to possess fixed nitrogen, molten inorganic phosphorus and produce single bacterial classification plant growth-promoting microbial inoculum of growth hormone ability, can make full use of the abundantest insoluble inorganic phosphate of content in the soil, when possessing multiple short living ability, solve antagonism and the inconsistent problem of nutritional needs between the bacterial classification that exists in many bacterial classifications microbial inoculum; Bacterial classification is single, and preparation technology is easy, and is lower to the requirement of production and sealed in unit; Growth-promoting functions to clover, oat, sainfoin seedling is remarkable, and many bacterial classifications microbial inoculum product of equal fertilizer efficiency is cheap, is easy to apply.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for description and interpretation the present invention, and be not used in qualification the present invention.
Embodiment 1
Klebsiella pneumonia (
Klebsiella pneumoniae) separation, evaluation and purifying: the RSN19 bacterial strain with no nitrogen solid medium and the molten inorganic phosphorus substratum of PKO from the sainfoin of surface sterilization (
Onbrychis viciaefoliacv.) separate in the root nodule, the bacterial strain behind the purifying is identified the General Definition that meets enterobacteriaceae through morphology.The bacterial strain pure growth was accredited as Klebsiella pneumonia (Klebsiella pneumoniae) (No. the 084th, 2010 little searchings) through Chinese Academy of Sciences microorganism identification preservation center in 2010; This bacterial strain has tolerance to 80mg/L kantlex and 200mg/L penbritin.RSN19 bacterial strain nitrogenase activity reaches 6695 ± 174 nmol C2H2 d-1, and the amount of phosphorus dissolved of 4d (calcium phosphate) is 15.29 ± 0.46mg/L, and the growth hormone output of 6d is 8.38 mg/L.At no nitrogen, only provide in the sand culture experiment under the calcium phosphate condition, the clover seedling of using behind the RSN19 bacterium liquid 60d increases by 119% than the blank plant biomass of not executing bacterium liquid, plant height increases by 53%, the number of blade increases by 95%, is used for seed soaking, can significantly accelerate the elongation of radicle.Under the condition that nitrogen phosphorus lacks, plant there is clearly growth-promoting functions.
Embodiment 2
A kind of preparation method of plant growth-promoting microbial inoculum, in carrying out according to the following step:
(1) Klebsiella pneumonia (
Klebsiella pneumoniae) RSN19 is inoculated in YMA (yeast N.F,USP MANNITOL-agar) solid medium, at 26 ℃～28 ℃ activation 18～24h;
(2) activation after
Klebsiella pneumoniaeRSN19 is inoculated in and carries out fermentation culture in the liquid nutrient medium, and temperature is 25 ℃～29 ℃, is cultured in every milliliter of fermented liquid
Klebsiella pneumoniaeRSN19 bacterium number is 4～6 10
10Individual;
(3) with in the above-mentioned steps (2)
Klebsiella pneumoniaeThe zymocyte liquid of RSN19, aseptic liquid nutrient medium and concentration are that 10% pH 7.0 aseptic phosphoric acid buffers are 6: 3: 1 mixed according to volume ratio; With aseptic aluminium foil bag or the encapsulation of aseptic Polypropylene Bag, make single bacterial classification liquid bacterial agent;
(4) will state the liquid bacterial agent of preparation in the step (3) and aseptic peat dust is 1: 6 mixed according to volume ratio, makes solid microbial.
The liquid nutrient medium of described growth promoting bacteria agent consists of, according to the KH of weight fraction ratio by 0.5～1 part
2PO
4, 1～2 part yeast powder, 5～10 parts glucose, 0.2～0.4 part MgSO
4, 0.05～0.1 part NaCl, 900～1000 parts water is formed, and wherein the pH value of liquid nutrient medium is 6.4～7.4.
Embodiment 3
The sand training growth-promoting functions of microbial inoculum of the present invention under control nitrogen, phosphorus nutrition condition
One, experiment material
1.1 test materials
Liquid bacterial agent of the present invention: Klebsiella pneumonia (
Klebsiella pneumoniae), its microbial preservation number is: CGMCC No. 4128.
1.2 vegetable material
Alfalfa Long Dong (Medicogosativa. cv. LongDong) and queen's (Medicogo. sativa. cv. Queen) seed, Gansu sainfoin (Qnobrychisspp. cv. Gansu) and oat (Calibre) (Avena. sativa L. Calibre) seed is provided by key lab of the grass cultivation ecosystem the Ministry of Education, physical purity of seed 98%, seed is with 70% ethanolic soln sterilization 5min.For eliminating the influence to experimental result of kind of band root nodule bacterium and vinelandii, seed soaks vernalization through sterilized water, removes kind of a skin, after sowing after the aseptic deionized water flushing 5～8 times.
1.3 husky cultivation nutrient fluid
Hoagland's complete nutrition liquid: macroelement: Ca (NO3) 24H2O 1417mg/L; KNO3 607mg/L; MgSO4 493mg/L; (NH4) 3PO4 115mg/L; Trace element: H3BO3 2.86mg/L; MnCl24H2O 1.81mg/L; ZnSO47H2O 0.22mg/L; CuSO45H2O 0.08mg/L; H2MoO4H2O 0.02mg/L; FeSO4 50mg/L.Nutritive medium is diluted to aseptic deionized water that to regulate the pH value with NaOH (1mol/L) or HCl (1mol/L) after 1/4 concentration be 7.0 ± 0.2.
Hoagland's does not have the without phosphorus nutritive medium of nitrogen based on Hoagland's complete nutrition liquid, changes the KNO3 607mg/L in the prescription into KCl 582mg/L, and prepares after removing (NH4) 3PO4, and regulator solution pH value to 7.0 ± 0.1.
Two, experimental technique
2.1. test design
Tested in April, 2010 and carry out in culturing room of grass cultivation institute of Gansu Agriculture University,, cultivation matrix uses cleaning river sand, after HCl soaks 48h, wash 6 times, and with distilled water flushing 3 times, also dry sterilization 6h is standby in 150 ℃ of oven dry at last.The dry river sand of the 280g that packs in the plastic culture cup of diameter 7cm, volume 400ml with the Shamian Island leveling, is selected 10 full seeds of the same size with tweezers and is evenly put in the fine sand surface, covers husky 20g.Each is handled seedling pouring with this product liquid microbial inoculum of 100 times of sterilized water dilutions (viable count〉106cfu/ml), in the sand matrix of cultivation usefulness, add the calcium phosphate of 20g/kg, do not have the without phosphorus nutritive medium pouring of nitrogen with 1/4Hoagland's.Handle not connecing bacterium liquid, only do not have the processing of the without phosphorus nutritive medium pouring of nitrogen as blank (CK1) with 1/4Hoagland's; Processing with the pouring of 1/4Hoagland's complete nutrition liquid contrasts (CK2) as sufficient nutrition.Every processing repeats for 4 times.Measure each potted plant moisture content with weighing method in the treating processes, and keep the skin wet to 70% of husky matrix water retaining capacity with sterilized water.Handle and to grow behind the 50d and the mensuration of morphological index.
The testing index of manure trial and method
The seedling rate of living: each is handled the potted plant statistics seedling number of living that washes out behind the seedling, calculate the seedling rate of living.
Plant height and main root are long: each is handled potted plant middle picked at random 4 strain seedling and measures plant height and main root length.To clean plant and be tiled on glass plate, measure the natural height of seedling with ruler, after with tweezers that plant root is stretching again, measure main root length.
Single leaf area and single-strain blade number: get individual plant record lobe numbers, single leaf area adopts retouches the shape weighting method.
Plant individual plant volume and individual plant root volume: selected seedling is placed on the filter paper, water is blotted, adopt the graduated cylinder drainage to measure plant volume and root volume.
The plant biomass accumulation: round plant in baking oven, 80 ℃ dry to constant weight, and weigh up the plant total dry weight with electronic balance.
2.2 data analysis
Testing data is carried out statistical study with SPSS16.0 software with the LSD method.
Three. test-results
3.1 microbial inoculum of the present invention is to the influence of survival rate of seedling under the low available phosphorus condition
After handling 50d, use microbial inoculum of the present invention and can significantly improve for the survival rate of seedling that studies thing.Long Dong and queen clover survival rate of seedling improve 32% and 28% respectively than CK1, improve 8% and 12% respectively than CK2, make sainfoin and avenaceous survival rate of seedling improve 27%～44% respectively than CK1
Table 1 microbial inoculum of the present invention is to the influence (%) of clover, oat and sainfoin survival rate of seedling
CK1 is that 1/4Hoagland's does not have the without phosphorus nutritive medium of nitrogen+calcium phosphate and handles, and CK2 is that 1/4Hoagland's complete nutrition liquid is handled, with it as the generally contrast under the nutritional condition usually.Down together.
3.2 this product microbial inoculum is to crop seedling plant height and the long influence of main root
After handling 50d, this product microbial inoculum makes Gansu Province average plant height of east clover increase 68.5% and 26.67% respectively than CK1 and CK2, make the queen clover exceed 70.92% and 35.9%, make the plant height of oat seedlings exceed 49.08%, 40.54%, significant difference (P＜0.05) than CK1, CK2 than CK1 and CK2.And can make Gansu sainfoin plant height improve 21.14% and 17.35% respectively than CK1 and CK2.
Table 2 microbial inoculum of the present invention is to the influence (unit: cm) of clover, oat and sainfoin seedling plant height
Gansu Province east clover root length of inoculating this product increases 50.2% and 18.98% respectively than CK1 and CK2, makes queen clover root length exceed 56.23% and 30.5% than CK1 and CK2, makes grow tall out CK1 and CK2 of the root of oat seedlings exceed 76.51% and 53.09%.Make Gansu sainfoin root length improve 25.69% and 91.62% respectively, significant difference (P＜0.05) than CK1 and CK2.
Table 3 microbial inoculum of the present invention is to the influence (unit: cm) of clover, oat and sainfoin main root length
3.3 this product microbial inoculum is to the influence of crop seedling list leaf area, single-strain blade number and biomass accumulation
After using this product microbial inoculum, the leaf area ratio CK1 of two kinds of clovers improves 123.33%～353.76%, improves 36.73～66.63% than CK2; The processing of Gansu its CK1 of sainfoin leaf area ratio and CK2 increases by 107.36% and 42.76% respectively; Its CK1 of leaf area ratio of oat (Calibre) seedling and the processing of CK2 increase by 87.40% and 71.99% respectively, and difference all has statistical significance (P＜0.05).
Table 4 microbial inoculum of the present invention is to the influence (unit: cm of the single leaf area of clover, oat and sainfoin
2)
This product microbial inoculum also has obvious facilitation to the number of blade of plant, and the number of blade of two kinds of clovers improves 53.75%～26.63% than the processing of its CK1; The Gansu sainfoin number of blade increases by 118.52% and 56.69% respectively than the processing of its CK1 and CK2; The number of blade of oat (Calibre) seedling increases by 39.95% and 33.80% respectively than the processing of its CK1 and CK2, and difference all has statistical significance (P＜0.05).
Table 5 microbial inoculum of the present invention is to the influence (unit: blade/strain) of clover, oat and sainfoin single-strain blade number
Compare with CK1, use two kinds of clover total dry weights of this product microbial inoculum on average to increase more than 90.18%.Gansu sainfoin dry weight increases by 86.33%, increases by 26.19% than CK2 than CK1.Oat plant dry weight increases by 217.54% than the processing of its CK1, and difference has statistical significance.
Table 6 microbial inoculum of the present invention is to the influence (unit: mg/strain) of clover, oat and sainfoin biomass of individual tree dry weight
3.4 this product microbial inoculum is to the influence of seedling individual plant volume
Table 7 microbial inoculum of the present invention is to the influence (unit: cm of clover, oat and sainfoin plant volume
3/ strain)
After using this product microbial inoculum, the seedling individual plant volume ratio CK1 of Gansu Province east clover and queen clover improves 79.23%～131.87%, improve 36.71% and 59.44% than CK2, compare with CK1 and CK2 after Gansu sainfoin and oat (Calibre) the inoculation microbial inoculum of the present invention, the plant volume increases more than 76.48%, and difference has statistical significance (P＜0.05).
Four. conclusion
Microbial inoculum of the present invention has remarkable promoter action to the growth of plant under the nutritional condition of low-phosphorous low nitrogen, mainly show remove plant phosphorus, the nitrogen nutritional deficiency is coerced, and promotes the growth of plant leaf and root system, promotes the accumulation of plant biomass.
Above testing data shows that microbial inoculum of the present invention has following advantage
Microbial inoculum of the present invention is to possess fixed nitrogen, molten inorganic phosphorus and produce single bacterial classification plant growth-promoting microbial inoculum of growth hormone ability, can make full use of the abundantest insoluble inorganic phosphate of content in the soil, when possessing multiple short living ability, solve antagonism and the inconsistent problem of nutritional needs between the bacterial classification that exists in many bacterial classifications microbial inoculum.
Bacterial classification is single, and preparation technology is easy, and is lower to the requirement of production and sealed in unit.
Growth-promoting functions to clover, oat, sainfoin seedling is remarkable, and many bacterial classifications microbial inoculum product of equal fertilizer efficiency is cheap, is easy to apply.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
- Klebsiella pneumonia ( Klebsiella pneumoniae), its microbial preservation number is: CGMCC No.4128; The morphological feature of described Klebsiella pneumonia is: the straight-bar bacterium, diameter 0.5～0.8 μ m, long 3.2～5.0 μ m, single distribution, pod membrane is arranged, Gram-negative, do not move, be grown on the YMA medium and to produce a large amount of polysaccharide of circular translucent colony justacrine, the effect of fixed nitrogen, dissolving inorganic phosphorus is arranged, can glucose, sucrose, Zulkovsky starch and N.F,USP MANNITOL is sole carbon source.
- Klebsiella pneumonia ( Klebsiella pneumoniae), microbial preservation number is: CGMCC No. 4128, and as the application in the plant growth-promoting microbial inoculum.
- 3. the preparation method of a plant growth-promoting microbial inoculum is characterized in that carrying out according to the following step:(1) Klebsiella pneumonia ( Klebsiella pneumoniae) in the RSN19 inoculation medium, at 26 ℃～28 ℃ activation 18～24h;(2) activation after Klebsiella pneumoniaeRSN19 is inoculated in and carries out fermentation culture in the liquid nutrient medium, and temperature is 25 ℃～29 ℃, is cultured in every milliliter of fermented liquid Klebsiella pneumoniaeRSN19 bacterium number is 4～6 10 10Individual;(3) with in the above-mentioned steps (2) Klebsiella pneumoniaeThe zymocyte liquid of RSN19, aseptic liquid nutrient medium and aseptic phosphoric acid buffer are 4～8: 2～4 according to volume ratio: 1 mixed, and single bacterial classification liquid bacterial agent is made in encapsulation;(4) will state the liquid bacterial agent of preparation in the step (3) and aseptic peat dust is 1: 4～8 mixed according to volume ratio, makes solid microbial.
- 4. a kind of plant growth-promoting microbial inoculum according to claim 3 is characterized in that the liquid nutrient medium of described growth promoting bacteria agent consists of, according to the KH of weight fraction ratio by 0.5～1 part 2PO 4, 1～2 part yeast powder, 5～10 parts glucose, 0.2～0.4 part MgSO 4, 0.05～0.1 part NaCl, 900～1000 parts water is formed, and wherein the pH value of liquid nutrient medium is 6.4～7.4.
- 5. a kind of plant growth-promoting microbial inoculum according to claim 3 is characterized in that the middle zymocyte liquid of described step (3), aseptic liquid nutrient medium and aseptic phosphoric acid buffer are 6: 3: 1 mixed according to volume ratio.
- 6. a kind of plant growth-promoting microbial inoculum according to claim 3 is characterized in that middle liquid bacterial agent of described step (4) and aseptic peat dust volume ratio are 1: 6.
- 7. a kind of plant growth-promoting microbial inoculum that is prepared from according to claim 3, the application in the molten phosphorus of fixed nitrogen.
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| CN102363758A (en) * | 2011-10-19 | 2012-02-29 | 黑龙江大学 | Azotobacteria separated from termite intestines |
| CN102965296A (en) * | 2012-02-10 | 2013-03-13 | 广西壮族自治区农业科学院微生物研究所 | Nitrogen-fixing Klebsiella sp. with high anti-glyphosate activity and its application |
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| US10555532B2 (en) | 2013-03-15 | 2020-02-11 | Spogen Biotech Inc. | Plant growth-promoting bacteria and methods of use |
| US10836800B2 (en) | 2014-09-17 | 2020-11-17 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| US9845342B2 (en) | 2014-09-17 | 2017-12-19 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| US11905315B2 (en) | 2014-09-17 | 2024-02-20 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| US10407472B2 (en) | 2014-09-17 | 2019-09-10 | Spogen Biotech Inc. | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria |
| CN107021828A (en) * | 2017-05-08 | 2017-08-08 | 福建农林大学 | A kind of Jujun grasses azotobacteria fertilizer and preparation method thereof |
| CN109777751A (en) * | 2019-01-21 | 2019-05-21 | 四川清和福生生物工程有限公司 | A kind of plant growth-promoting bacteria slow-releasing granules and its preparation method and application |
| CN112501084A (en) * | 2020-12-22 | 2021-03-16 | 山东农业大学 | Rhizosphere probiotic Klebsiella ZH07 and application thereof |
| CN112501084B (en) * | 2020-12-22 | 2022-04-08 | 山东农业大学 | Rhizosphere probiotic Klebsiella ZH07 and application thereof |
| CN115786166A (en) * | 2022-08-15 | 2023-03-14 | 湖南农业大学 | Klebsiella pneumoniae and application thereof |
| CN115786166B (en) * | 2022-08-15 | 2023-12-01 | 湖南农业大学 | Klebsiella pneumoniae and application thereof |
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