CN107236672A - Breed breeding method of the rhizoma Gastrodiae with halimasch - Google Patents
Breed breeding method of the rhizoma Gastrodiae with halimasch Download PDFInfo
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Abstract
The present patent application belongs to cultivating bacterial spawn technical field, specifically discloses a kind of breeding method for breeding rhizoma Gastrodiae with halimasch, comprises the following steps：（1）Compost dispensing,（2）Compost processing,（3）Inoculation,（4）Bottling,（5）Culture.Compared with prior art, this method is largely effective when solving at present using bottled production Armillaria mellea, the problem of fed difficult, easy ponding, and amount of water hardly possible is controlled, aeration is poor；This method can adjust pH value and temperature, further promote the growth of halimasch during bottled culture Armillaria mellea simultaneously.
Description
Technical field
The invention belongs to cultivating bacterial spawn technical field, a kind of breeding method for breeding rhizoma Gastrodiae with halimasch is specifically disclosed.
Background technology
Rhizoma Gastrodiae（Scientific name：Gastrodia elata Bl.）, also known as rhizoma gastrodiae, solely shake sesame, from it is female, close and superintended and directed from grass, god's grass, ghost
Postal, Mokpo, RHIZONA GASTRODIAE, DINGFENGCAO, Bai Longpi etc., Gastrodia herbaceos perennial, its root-like stock are plump, no greenery, capsule
Obovate elliptic, often with stem tuber or seminal propagation.Its rhizome be used as medicine to treat have a dizzy spell, extremity numbness, child convulsion
It is rare Chinese medicine Deng disease, treatment headache and insomnia is shared with the precious ganoderma lucidum of fine jade.That To Be Protected from Heat is not cold for rhizoma Gastrodiae, in winter temperature as little as -30
DEG C, as long as rhizoma Gastrodiae grown layer temperature is not less than -5 DEG C, rhizoma Gastrodiae can still pass the winter safely, but summer temperature is not higher than 28 DEG C, and temperature surpasses
When crossing 30 DEG C, the normal production of rhizoma Gastrodiae can be influenceed.
Rhizoma Gastrodiae can not carry out photosynthesis, and its stem tuber is dived and occupied in soil throughout the year, because itself does not absorb soil directly
Nutrient and the organ of manufacture nutrition, rhizoma Gastrodiae rely primarily on halimasch supply nutrition, ability normal growth and development and breeding, therefore cultivate
Halimasch is the primary work for breeding rhizoma Gastrodiae.Halimasch is symbiotrophy relation with rhizoma Gastrodiae, and rhizoma Gastrodiae can only absorb by halimasch
Nutriment in soil, halimasch can also regard Gastrodia Elata Stem Tuber or epidermal tissue as nutrition.Halimasch is with mycelia or with shape
The state of shoestring is saprophytic or colonizes in rotten wood and root living, and the young tender part of its shoestring, which gradually extends, expands to Gastrodia Elata Stem Tuber
On, and stem tuber dermal contact, and invade stem tuber number layer epidermal cell and supply nutrition for rhizoma Gastrodiae.
Armillaria mellea has the form such as Liquid Culture strain and solid culture strain in production, and gastrodin cultivation is mainly used
Fixed strain；Solid Armillaria mellea is mainly in weed tree sawdust, branch, wheat bran, corn flour etc. using bottled or packed production
Inoculated and cultured on the solid culture medium of preparation, treat mycelia and shoestring cover with culture medium production rhizoma Gastrodiae Armillaria mellea.Make
During with bottled production Armillaria mellea, because the water content of compost needs to be maintained between 65% -70% so that compost
It is overly wet to be difficult bottling；And Compost moisture content it is higher when be loaded into bottle, easily make the elutriation of compost go out to form ponding, shadow
Ring the growth of halimasch；Added water if first compost is fitted into bottle, be difficult to grasp amount of water also easy ponding；Incubation
In, if water is not enough also not easy to add water.In addition, when using bottled production Armillaria mellea, throughput is less in bottle also can
Cause halimasch growth slower, halimasch can not utilize carbon dioxide, need to absorb more oxygen and arrange in incubation
Go out carbon dioxide, the normal growth of halimasch can be suppressed if the excessive oxygen of carbon dioxide content lacks.Meanwhile, halimasch can not
Photosynthesis is carried out, if Armillaria mellea culture is carried out under periods of direct sunlight, the ultraviolet in daylight can carry out sterilization work to it
With, but its moisture will drastically evaporate, the culture of influence halimasch bacterium branch.
The content of the invention
The purpose of the present invention is providing a kind of breeding method for breeding rhizoma Gastrodiae with halimasch, to solve to use bottled life at present
When producing Armillaria mellea, the problem of fed difficult, easy ponding, and amount of water hardly possible is controlled, aeration is poor.
In order to achieve the above object, base case of the invention is：Breed breeding method of the rhizoma Gastrodiae with halimasch, including with
Lower step：
（1）Compost dispensing, includes the composition of following mass fraction：Wood chip 80-85 parts of Qinggang, 10-12 parts of wheat bran, corn flour
5-8 parts, 0.2-0.3 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, 1-2 parts of agar, 8-10 parts of glutinous rice flour；
（2）Compost processing, adds water infusion, the mass fraction of water is 75-80 parts, is treated by the glutinous rice flour in dispensing and corn flour
After glutinous rice flour and corn flour curing, agar is added into infusion into soup；After agar is melted, 40-45 DEG C is cooled to soup, will
Other dispensings, which are added, to be stirred evenly in soup and is cooled to normal temperature；
（3）Inoculation, it is sponge sheet thick 1-2mm to cut thickness, and sponge sheet is cleaned and wrung out using alcoholic solution；In sponge sheet
Surface fill the compost after processing, then halimasch is seeded on the sponge sheet for filling compost；
（4）Bottling, sponge sheet after inoculation is put into seed bottle, and polylith sponge sheet can be loaded in a seed bottle until by bacterium
Plant bottled full；Then the bottle stopper being made using porous flexible plastic is by strain bottle closure；
（5）Culture, culture apparatus is positioned under daylight, and one layer of river sand is spread in the tank of culture apparatus, plants many in river sand
Strain pasture and water, and in tank load normal temperature running water, make originally water submerged pasture and water；Then the seed bottle after sealing is inverted solid
It is scheduled in the bottle clip of culture apparatus, the motor of Primary culture device rotates transverse axis, seed bottle is rotated with rotating bezel；
The culture apparatus includes tank, support, rotating bezel, motor and cross bar, and the support is fixed on the side of tank, support
It is provided with transverse axis；The rotating bezel is two, and rotating bezel is fixedly and coaxially connected with transverse axis, and bearing piece is provided between two rotating bezels,
The bottom of rotating bezel is located in tank；The cross bar is multiple, and the two ends of cross bar rotate connection respectively at two rotating bezels, multiple
Cross bar is respectively positioned on the same circumference at rotating bezel edge；Bottle clip of the cross bar provided with clamping seed bottle, bottle clip is rotated with cross bar to be connected
Connect, the output shaft of transverse axis and motor is coaxially connected.
The operation principle of this base case is：Step（1）In, glutinous rice flour, glutinous rice flour are added in compost dispensing
Containing protein, fat, carbohydrate, calcium, phosphorus, iron, vitamin B1, vitamin B2, nicotinic acid and starch etc., nutriment enriches, can
The certain nutriment of supplement is grown for halimasch.In addition, glutinous rice flour, which is met, has stronger stickiness after water, compost dispensing can be binded
Together.Step（2）In, first by glutinous rice flour and corn flour infusion so that its nutrition fully can be absorbed by Armillaria mellea, together
When or glutinous rice flour give full play to its stickiness.Step（3）By the way that compost is coated on relatively thin sponge sheet, sponge sheet can be abundant
Absorb the moisture in compost；Due to having added glutinous rice flour in compost, compost can be attached on sponge sheet；Compost sticks
On sponge sheet, the systemic water of sponge sheet can be added on compost at any time.Step（4）The middle sea that will fill compost
Continuous piece is fitted into seed bottle, on the one hand loads very convenient, will not cause inconvenient bottling due to compost excess moisture, separately
One side sponge sheet can be by moisture absorption, and the compost water analysis that effectively prevent in seed bottle goes out to produce ponding；Using porous
Bottle stopper is made by strain bottle closure in soft plastic, porous soft plastic it is excessively aqueous preferably, will not absorb causes bottleneck quilt compared with juicy
Sealing can make seed bottle aeration preferable.
Step（5）In, the tank of culture apparatus spreads one layer of river sand, and many plants of pasture and water are planted in river sand, and built-in in tank
Enter the running water of normal temperature, make originally water submerged pasture and water；Pasture and water photosynthesis, it will constant absorption carbon dioxide simultaneously produces oxygen,
For the growth supplemental oxygen of halimasch, the carbon dioxide produced during halimasch growth is also absorbed.Seed bottle after sealing is inverted
It is fixed in the bottle clip of culture apparatus, the motor of Primary culture device rotates transverse axis, seed bottle is rotated with rotating bezel.Due to
Support is fixed on the side of tank, and support is provided with transverse axis；The output shaft of transverse axis and motor is coaxially connected；Motor, which is rotated, can make horizontal stroke
Axle is rotated, so that rotating bezel is rotated.Because rotating bezel is two, rotating bezel is fixedly and coaxially connected with transverse axis, two rotating bezels
Between be provided with bearing piece, the bottom of rotating bezel is located in tank；A part for rotating bezel can be continued into the water of tank.Cross bar
Two ends rotate connection respectively at two rotating bezels, and multiple cross bars are respectively positioned on the same circumference at rotating bezel edge；Cross bar is provided with
The bottle clip of seed bottle is clamped, bottle clip is rotated with cross bar and is connected；In rotating bezel rotation process, the cross bar positioned at rotating bezel edge will
Moved in a circle with rotating bezel, be inverted the seed bottle being fixed in the bottle clip of culture apparatus and move in a circle therewith.Due to cross bar
Rotate and be connected with rotating bezel, the rotation of bottle clip and cross bar is connected, make during seed bottle moves in a circle, due to seed bottle gravity
Effect, seed bottle bottleneck will all the time down.Seed bottle is during moving in a circle, it will in the water of immersion tank, water
It will enter at bottle stopper in seed bottle, can be kept the skin wet for the growth of halimasch；It is many in seed bottle after seed bottle leaves water
Remaining water will flow out along bottle stopper, prevent from producing ponding in seed bottle so that halimasch growth is preferable.Seed bottle is doing circumference fortune
In dynamic process, it will produce a little air-flow so that air enters in seed bottle by bottle stopper so that seed bottle ventilation is good.
It is provided with culture apparatus on multiple cross bars, each cross bar and is provided with bottle clip, therefore culture apparatus can the multiple bacterium of batch cultur
Bottle is planted, the culture efficiency that resource improves halimasch is effectively saved.
The beneficial effect of this base case is：In this cultural method, glutinous rice flour is added in compost, is on the one hand carried
The high stickiness of compost, on the other hand adds nutriment for compost.This cultural method fills sponge using compost
Piece is bottled, and one is to make bottling more convenient quick, and two be the effective moisture pinned in compost, three be can avoid because
Compost moisture content is higher, produces ponding after being fitted into bottle, four be to be kept the skin wet at any time for compost.Adopted in this cultural method
The bottle stopper being made of porous flexible plastic is by strain bottle closure so that bottle stopper will not ponding, aeration is good in seed bottle.This culture
Method is cultivated seed bottle using homemade culture apparatus so that seed bottle is in incubation, and aeration is good, water
Ponding will not be produced in abundance, and seed bottle；Tap water at normal temperature is used in addition, and plants pasture and water, and one is pasture and water photosynthesis, can
The carbon dioxide produced in halimasch incubation is absorbed, and produces oxygen and is supplied to halimasch；Two be that the pH value of running water exists
6.5-7, plantation pasture and water can reduce the pH value of running water, be adapted to pH value needed for halimasch growth；Three be that tap water at normal temperature can adjust
The temperature of halimasch growth, is suitable for the growth of halimasch.Compared with prior art, largely effective solve of this method makes at present
During with bottled production Armillaria mellea, the problem of fed difficult, easy ponding, and amount of water hardly possible is controlled, aeration is poor；Simultaneously
This method can adjust pH value and temperature, further promote the growth of halimasch during bottled culture Armillaria mellea.
Preferred scheme one：Based on scheme preferred, step（1）In, the composition of compost is according to following mass fraction
Prepare：It is 80 parts of Qinggang wood chip, 10 parts of wheat bran, 5 parts of corn flour, 0.2 part of potassium dihydrogen phosphate, 0.1 part of magnesium sulfate, 1 part of agar, glutinous
9 parts of ground rice.Understand that this dispensing can make halimasch growth conditions more by testing inspection.
Preferred scheme two：Based on scheme preferred, step（3）In, it is sponge sheet thick 1mm, sponge to cut thickness
After piece cleaning, make to fill compost outside sponge sheet in immersion compost.Sponge sheet space-consuming thick 1mm is smaller, and can
The water of absorption is enough；After sponge sheet cleaning, make to fill compost outside sponge sheet in immersion compost so that compost is assembled
Process faster, it is better.
Preferred scheme three：Based on scheme preferred, step（5）In, culture apparatus also includes bearing, the cross bar
Two ends are rotated with rotating bezel by bearing and are connected.Bearing can reduce cross bar and rotate the friction being connected with rotating bezel, can fill culture
Put running more preferably.
Preferred scheme four：Based on scheme preferred, step（5）In, culture apparatus also includes decelerator, the motor
Connected with cross bar by decelerator.Decelerator can effectively reduce the rotating speed of cross bar, more suitable for the culture of seed bottle.
Preferred scheme five：Based on scheme preferred, step（5）In, the transverse axis is eight, and eight transverse axis uniformly divide
Cloth is on the circumference of rotating bezel outer.Can eight seed bottles of batch cultur, culture efficiency more preferably, eight seed bottles can be made equal
Even to be distributed on the circumference of rotating bezel outer, culture effect is more preferably.
Brief description of the drawings
Fig. 1 is the structural representation of culture apparatus in the present invention；
Fig. 2 is the side view of culture apparatus in the present invention.
Embodiment
Below by embodiment, the present invention is further detailed explanation：
Reference in Figure of description includes：Tank 10, support 20, transverse axis 21, rotating bezel 30, bearing piece 31, bearing 32, electricity
Machine 40, decelerator 41, cross bar 50, bottle clip 51.
Embodiment 1
The present embodiment discloses a kind of breeding method for breeding rhizoma Gastrodiae with halimasch, comprises the following steps：
（1）Compost dispensing, includes the composition of following mass fraction：80 parts of Qinggang wood chip, 10 parts of wheat bran, 5 parts of corn flour, phosphorus
0.2 part of acid dihydride potassium, 0.1 part of magnesium sulfate, 1 part of agar, 9 parts of glutinous rice flour；
（2）Compost processing, adds water infusion, the mass fraction of water is 75-80 parts, is treated by the glutinous rice flour in dispensing and corn flour
After glutinous rice flour and corn flour curing, agar is added into infusion into soup；After agar is melted, 40-45 DEG C is cooled to soup, will
Other dispensings, which are added, to be stirred evenly in soup and is cooled to normal temperature；
（3）Inoculation, it is sponge sheet thick 1mm to cut thickness, and sponge sheet is cleaned and wrung out using alcoholic solution；Sponge sheet is soaked
Enter makes to fill compost outside sponge sheet in compost, then halimasch is seeded on the sponge sheet for filling compost；
（4）Bottling, sponge sheet after inoculation is put into seed bottle, and polylith sponge sheet can be loaded in a seed bottle until by bacterium
Plant bottled full；Then the bottle stopper being made using porous flexible plastic is by strain bottle closure；
（5）Culture, culture apparatus is positioned under daylight, is spread one layer of river sand in the tank 10 of culture apparatus, is planted in river sand
Many plants of pasture and water, and in the interior running water for loading normal temperature of tank 10, make originally water submerged pasture and water；Then the seed bottle after sealing is fallen
Put in the bottle clip 51 for being fixed on culture apparatus, the motor 40 of Primary culture device rotates transverse axis 21, makes seed bottle with rotating bezel
30 rotate；
As shown in Figure 1 and Figure 2, culture apparatus includes tank 10, support 20, rotating bezel 30, bearing 32, decelerator 41, the and of motor 40
Cross bar 50, support 20 is fixed on the side of tank 10, and support 20 is provided with transverse axis 21；Rotating bezel 30 be two, rotating bezel 30 with
Transverse axis 21 is fixedly and coaxially connected, and bearing piece 31 is provided between two rotating bezels 30, and the bottom of rotating bezel 30 is located in tank 10；Cross bar
50 be eight, and the two ends of cross bar 50 are rotated by bearing 32 respectively at two rotating bezels 30 to be connected, and eight cross bars 50 uniformly divide
On the same circumference for being distributed in the outer of rotating bezel 30；50 turns of bottle clip 51 of the cross bar 50 provided with clamping seed bottle, bottle clip 51 and cross bar
Dynamic connection；Transverse axis 21 is connected with motor 40 by decelerator 41.
Using above-mentioned cultural method culture Armillaria mellea, carrying out observed and recorded to incubation can obtain：This method culture
During bottled armillaria mellea strain, compost bottling process operation is simple, convenient, can uniformly fill compost and its contained humidity
Enter in seed bottle, and seeping phenomenon will not occur in bottle.During using culture apparatus culture bottled armillaria mellea strain, strain
Bottle bottleneck down, when seed bottle has just been clamped in bottle clip 51, has a small amount of water to be spilt from bottle mouth position always；Seed bottle is doing circumference fortune
During dynamic, when moving in screw clamp 10, the water in tank 10 can be rapidly filled with seed bottle, treat that seed bottle leaves the water surface, bacterium
Plant water unnecessary in bottle quickly can then spill from bottleneck.In addition during use culture apparatus culture bottled armillaria mellea strain, water
Grass carries out photosynthesis, absorbs the carbon dioxide of seed bottle generation, and discharges oxygen for halimasch production and application, due to bacterium
Plant bottle to move in a circle always, the ventilatory conditions in seed bottle are good, halimasch mycelia and shoestring growth are rapid uniform.
Knowable to being observed the Armillaria mellea turned out：Bottle in halimasch mycelia and shoestring be evenly distributed, it is intensive,
Armillaria mellea incubation time is shorter；Bottle in halimasch mycelia and shoestring growing way preferably, more luxuriant, the sweet ring at bottom of bottle
Bacterium distribution is slightly few, and dense growth has halimasch outside each sponge sheet in seed bottle bottle.
Embodiment 2
The difference of embodiment 2 and embodiment 1 is：
Step（1）In, the composition of compost is prepared according to following mass fraction：82 parts of Qinggang wood chip, 11 parts of wheat bran, corn flour
7 parts, 0.25 part of potassium dihydrogen phosphate, 0.15 part of magnesium sulfate, 1.5 parts of agar, 8 parts of glutinous rice flour.
Found by the method culture bottled armillaria mellea strain of the present embodiment, during seed bottle is cultivated, in bottle not
Can occur ponding, compost preferably absorption is on sponge sheet, and water is sufficient in seed bottle, and ventilation is good；Honey in seed bottle
Ring bacterium mycelia and shoestring growth are rapid, are evenly distributed and grow fine.In the incubation of seed bottle, compost small amount is from sponge
Come off on piece, during using culture apparatus culture seed bottle, seed bottle is after repeatedly water is entered, the compost on sponge sheet
Small amount drops to bottle mouth position.The bottled armillaria mellea strain turned out is observed, the halimasch mycelia and shoestring in bottle are long
Gesture is preferable, more luxuriant, and the halimasch distribution at bottom of bottle is slightly few, the halimasch distribution comparatively dense of bottle mouth position；In seed bottle bottle
Dense growth has halimasch outside each sponge sheet.
Embodiment 3
The difference of embodiment 3 and embodiment 1 is：
Step（1）In, the composition of compost is prepared according to following mass fraction：85 parts of Qinggang wood chip, 12 parts of wheat bran, corn flour
8 parts, 0.3 part of potassium dihydrogen phosphate, 0.2 part of magnesium sulfate, 2 parts of agar, 10 parts of glutinous rice flour.
Found by the method culture bottled armillaria mellea strain of the present embodiment, compost is being loaded to the process of seed bottle
In, seeping phenomenon will not occur in seed bottle, and water is sufficient, aeration is preferable；Compost on sponge sheet is preferably adsorbed
On the surface of sponge sheet.The bottled armillaria mellea strain turned out is observed, the halimasch mycelia and shoestring growing way in bottle compared with
Good, more luxuriant, the halimasch distribution at bottom of bottle slightly lacks in seed bottle bottle that dense growth has sweet ring outside each sponge sheet
Bacterium.
Embodiment 4
The difference of embodiment 4 and embodiment 1 is：
Step（3）In, it is sponge sheet thick 2mm to cut thickness, and after sponge sheet cleaning, compost is filled to the appearance of sponge sheet
Face.
Found by the method culture bottled armillaria mellea strain of the present embodiment, the moisture in seeded process, compost compared with
Difficulty is all introduced into sponge sheet, and the part water in compost is remained after inoculation, causes the moisture in seed bottle most opening
It is not enough enough when beginning.The bottled armillaria mellea strain turned out is observed, halimasch mycelia and shoestring growing way in bottle
Preferably, more luxuriant, the distribution of halimasch at bottom of bottle slightly lacks in seed bottle bottle that dense growth has honey outside each sponge sheet
Ring bacterium.
Comparative example 1
The difference of comparative example 1 and embodiment 1 is：Step（1）In, it is added without glutinous rice flour.
Found by the method culture bottled armillaria mellea strain of this comparative example, during seed bottle is cultivated, compost
Preferably it can not adsorb on sponge sheet, compost is relatively large to come off from sponge sheet；Using culture apparatus culture seed bottle
During, seed bottle is after repeatedly water is entered, and compost on sponge sheet is relatively large to drop to bottle mouth position.It is bottled to what is turned out
Armillaria mellea is observed, and the halimasch mycelia and shoestring growing way in seed bottle are poor, skewness；Sweet ring at bottom of bottle
Bacterium distribution is slightly few, and the halimasch of bottle mouth position is densely distributed；Turned out compared to embodiment 1 turn out come Armillaria mellea compared with
Difference.
Comparative example 2
The difference of comparative example 2 and embodiment 1 is：Step（3）In, without using sponge sheet, directly amount compost is scooped into seed bottle
In.
Found by the method culture bottled armillaria mellea strain of this comparative example, during seed bottle is cultivated, in bottling
Afterwards, there is ponding in blake bottle；During using culture apparatus culture seed bottle, compost is easily accumulated in bottle mouth position, and
Moisture in compost is easy to run off, the water deficient for causing halimasch to grow.The bottled armillaria mellea strain turned out is seen
Examine halimasch mycelia in discovery, seed bottle and shoestring growing way is excessively poor, and skewness.
Comparative example 3
The difference of comparative example 3 and embodiment 1 is：Step（5）In, the tank 10 of culture apparatus does not spread river sand, does not also plant water
Grass, only adds appropriate tap water at normal temperature.
Found by the method culture bottled armillaria mellea strain of this comparative example, in the mistake using culture apparatus culture seed bottle
Cheng Zhong, seed bottle aeration preferably, but is due to that halimasch production produces more carbon dioxide, so that near seed bottle
Carbon dioxide content is too high, and oxygen content is less, and the upgrowth situation of halimasch is not good in seed bottle.To the bottled sweet ring turned out
Bacterium strain is carried out it has been observed that halimasch mycelia and shoestring in bottle are evenly distributed, but not intensive enough.
Comparative example 4
The difference of comparative example 4 and embodiment 1 is：Step（5）In, seed bottle is cultivated without using culture apparatus, used
Conventional mode, seed bottle is placed and cultivated in the cool.
Found by the method culture bottled armillaria mellea strain of this comparative example, during seed bottle is cultivated, during beginning
Halimasch grows fine, and with the water loss in seed bottle, the growing way of halimasch is worse and worse.To the bottled honey turned out
Ring bacterium strain is carried out it has been observed that halimasch mycelia and shoestring in bottle are evenly distributed, but growing way is poor, more sparse.
By the bottled armillaria mellea strain to above-described embodiment 1-4, and comparative example 1-4 method culture, by experiment
Detection understand, using embodiment 1-4 method culture bottled armillaria mellea strain growing way preferably, and it is more intensive, be evenly distributed,
Planting each sponge sheet outside dense growth in bottle bottle has halimasch.
Claims (6)
1. breed breeding method of the rhizoma Gastrodiae with halimasch, it is characterised in that comprise the following steps：
（1）Compost dispensing, includes the composition of following mass fraction：Wood chip 80-85 parts of Qinggang, 10-12 parts of wheat bran, corn flour
5-8 parts, 0.2-0.3 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of magnesium sulfate, 1-2 parts of agar, 8-10 parts of glutinous rice flour；
（2）Compost processing, adds water infusion, the mass fraction of water is 75-80 parts, is treated by the glutinous rice flour in dispensing and corn flour
After glutinous rice flour and corn flour curing, agar is added into infusion into soup；After agar is melted, 40-45 DEG C is cooled to soup, will
Other dispensings, which are added, to be stirred evenly in soup and is cooled to normal temperature；
（3）Inoculation, it is sponge sheet thick 1-2mm to cut thickness, and sponge sheet is cleaned and wrung out using alcoholic solution；In sponge sheet
Surface fill the compost after processing, then halimasch is seeded on the sponge sheet for filling compost；
（4）Bottling, sponge sheet after inoculation is put into seed bottle, and polylith sponge sheet can be loaded in a seed bottle until by bacterium
Plant bottled full；Then the bottle stopper being made using porous flexible plastic is by strain bottle closure；
（5）Culture, culture apparatus is positioned under daylight, and one layer of river sand is spread in the tank of culture apparatus, plants many in river sand
Strain pasture and water, and in tank load normal temperature running water, make originally water submerged pasture and water；Then the seed bottle after sealing is inverted solid
It is scheduled in the bottle clip of culture apparatus, the motor of Primary culture device rotates transverse axis, seed bottle is rotated with rotating bezel；
The culture apparatus includes tank, support, rotating bezel, motor and cross bar, and the support is fixed on the side of tank, support
It is provided with transverse axis；The rotating bezel is two, and rotating bezel is fixedly and coaxially connected with transverse axis, and bearing piece is provided between two rotating bezels,
The bottom of rotating bezel is located in tank；The cross bar is multiple, and the two ends of cross bar rotate connection respectively at two rotating bezels, multiple
Cross bar is respectively positioned on the same circumference at rotating bezel edge；Bottle clip of the cross bar provided with clamping seed bottle, bottle clip is rotated with cross bar to be connected
Connect, the output shaft of transverse axis and motor is coaxially connected.
2. breed breeding method of the rhizoma Gastrodiae with halimasch as claimed in claim 1, it is characterised in that step（1）In, compost
Composition prepared according to following mass fraction：80 parts of Qinggang wood chip, 10 parts of wheat bran, 5 parts of corn flour, 0.2 part of potassium dihydrogen phosphate,
0.1 part of magnesium sulfate, 1 part of agar, 9 parts of glutinous rice flour.
3. breed breeding method of the rhizoma Gastrodiae with halimasch as claimed in claim 1, it is characterised in that step（3）In, cut thickness
Spend for sponge sheet thick 1mm, after sponge sheet cleaning, make to fill compost outside sponge sheet in immersion compost.
4. breed breeding method of the rhizoma Gastrodiae with halimasch as claimed in claim 1, it is characterised in that step（5）In, culture dress
Putting also includes bearing, and the two ends of the cross bar are rotated by bearing with rotating bezel to be connected.
5. breed breeding method of the rhizoma Gastrodiae with halimasch as claimed in claim 1, it is characterised in that step（5）In, culture dress
Putting also includes decelerator, and the motor and cross bar are connected by decelerator.
6. breed breeding method of the rhizoma Gastrodiae with halimasch as claimed in claim 1, it is characterised in that step（5）In, the horizontal stroke
Axle is eight, and eight transverse axis are evenly distributed on the circumference of rotating bezel outer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710389464.XA CN107236672B (en) | 2017-05-27 | 2017-05-27 | Cultivation method of Armillaria mellea for breeding rhizoma gastrodiae |
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| CN109729929A (en) * | 2019-03-25 | 2019-05-10 | 西南林业大学 | A method of bacterium material production Armillaria mellea is discarded using Rhizoma Gastrodiae |
| CN111019835A (en) * | 2019-11-01 | 2020-04-17 | 汉中市汉台区汉麓生物科技有限公司 | Method for separating Armillaria mellea from ambary endophytic fungi |
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