CN107311732A - A kind of cultivating white fungus culture medium and preparation method thereof - Google Patents
A kind of cultivating white fungus culture medium and preparation method thereof Download PDFInfo
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- 241000233866 Fungi Species 0.000 title claims abstract description 77
- 239000001963 growth medium Substances 0.000 title claims abstract description 77
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 68
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- 229910001868 water Inorganic materials 0.000 claims abstract description 55
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 39
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- 229930006000 Sucrose Natural products 0.000 claims abstract description 34
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 34
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 34
- 239000005720 sucrose Substances 0.000 claims abstract description 34
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 33
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 33
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000011511 Diospyros Nutrition 0.000 description 1
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- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 1
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- CHKVPAROMQMJNQ-UHFFFAOYSA-M potassium bisulfate Chemical compound [K+].OS([O-])(=O)=O CHKVPAROMQMJNQ-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to cultivating white fungus field, more particularly to a kind of cultivating white fungus culture medium and preparation method thereof.The culture medium that the present invention is provided, is made up of water with the raw material including following parts by weight of component：30~80 parts of cotton seed hulls；30~80 parts of wood chip；1~30 part of wheat bran；1~2 part of sucrose；0.5~3 part of gypsum；0.15~0.45 part of potassium dihydrogen phosphate；0.4~0.8 part of magnesium sulfate.The present invention optimizes design by constituent and content to cultivating white fungus culture medium, while having higher yields and quality when ensureing that it cultivates white fungus, reduces the consumption of cotton seed hulls in culture medium, improves the security of culture medium.Test result indicates that, the biological efficiency when culture medium provided using the present invention cultivates white fungus is more than 13%；The Thick many candies content for cultivating obtained white fungus is more than 20g/100g, and total sugar content is more than 73g/100g, and crude protein content is more than 10g/100g.
Description
Technical field
The invention belongs to cultivating white fungus field, more particularly to a kind of cultivating white fungus culture medium and preparation method thereof.
Background technology
China is Cotton Production state maximum in the world, and the planting scale of transgene cotton was accelerating to expand in recent years.I
The cultivated area of state's transgene cotton is from the 7000hm of 19972, rapidly increase to about 433.33 ten thousand hm of 20082, account for
The 70% of national cotton cultivated area.
Before cottonseed oil expression, seed shell is sloughed with rejuvenator, cotton seed hulls typically accounts for 40% or so of cottonseed weight.Cotton seed hulls
Nutritional ingredient it is many and content is high, data shows, cotton seed hulls contains crude fibre 37%~48%, pentosan 22%~25%, wooden
Quality 28%~32%, NFE 29.6%, ash content mineral matter 6.1%, its cellulose, lignin and nitrogen, phosphorus, potassium
Content is all higher than other stalks, also with some protein and mineral matter in the Cotton Seed chip infiltrated, and pH value is bordering on
Neutrality, not only water conservation but also ventilation, is the natural medium main material for cultivating white fungus in addition.
In recent years, it is a large amount of to be used as Cultivation use culture white fungus, such as GB/T29369-2012 white fungus production skill by the use of cotton seed hulls
Formula one described in art specification, the formula is by 82~88wt% of cotton seed hulls, 2~1wt% of 16~11wt% of wheat bran and land plaster
Composition.But as soil of transgenic Bt cotton spends the rapid expansion of planting scale, transgenic cotton seed shell is used in edible fungus culturing
It has been it is difficult to the problem of avoiding.Due to the importing of foreign gene, corresponding albumen can be expressed in cotton plants, while external source base
The insertion of cause may also lead to produce abnormal metabolism material in cotton body.Foreign gene, foreign protein and abnormal metabolism material all may be used
It can be accumulated due to the absorption of mycelia in fructification, so as to cause, to non-targeted biological influence, to produce bio-toxicity.Consider
To the security of the whole ecosystem, and without under clear and definite, substantial amounts of safety report, develop a kind of silver of low cotton seed hulls consumption
Ear culture medium for cultivating is very necessary.
The content of the invention
In view of this, it is an object of the invention to provide a kind of cultivating white fungus culture medium and preparation method thereof, the present invention is carried
While the culture medium of confession has higher yields and quality when ensureing that it cultivates white fungus, the use of cotton seed hulls in culture medium is reduced
Amount, improves the security of culture medium.
The invention provides a kind of cultivating white fungus culture medium, it is made up of water with the raw material including following parts by weight of component：
30~80 parts of cotton seed hulls；
30~80 parts of wood chip；
1~30 part of wheat bran；
1~2 part of sucrose；
0.5~3 part of gypsum；
0.15~0.45 part of potassium dihydrogen phosphate；
0.4~0.8 part of magnesium sulfate.
It is preferred that, the culture medium is made up of water with the raw material including following parts by weight of component：
40~50 parts of cotton seed hulls；
40~50 parts of wood chip；
5~10 parts of wheat bran；
1~1.2 part of sucrose；
0.8~1.1 part of gypsum；
0.18~0.2 part of potassium dihydrogen phosphate；
0.5~0.6 part of magnesium sulfate.
It is preferred that, the water content of the culture medium is 62~66wt%.
Invention also provides a kind of preparation method for preparing cultivating white fungus culture medium of the present invention, including following step
Suddenly：
A), cotton seed hulls, wood chip and wheat bran are soaked in water, wet feed is obtained；
B), the wet feed is mixed with sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water, fermentation is banked up, obtains white fungus
Culture medium for cultivating.
It is preferred that, the step a) is：
Cotton seed hulls, wood chip and wheat bran soak in 0.5~2wt% limewash, obtain wet feed.
It is preferred that, in step b), the time for banking up fermentation is 4~6 days.
It is preferred that, it is described to bank up in fermentation process every turning in 2~3 days once in step b).
It is preferred that, the step b) is：
Turning is carried out after the wet feed is banked up into fermentation 2~3 days, and adds sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate
And water, continue to bank up fermentation, obtain cultivating white fungus culture medium.
It is preferred that, the stockpile for banking up fermentation is trapezoidal heap, a height of 1~1.2cm of stockpile, a width of 2~3m of stockpile.
It is preferred that, it is provided with vent cap on the stockpile；A diameter of 12~15cm of the vent cap；The ventilation
The density of setting in duct is 1~2/m3。
Compared with prior art, the invention provides a kind of cultivating white fungus culture medium and preparation method thereof.The present invention is provided
Cultivating white fungus culture medium, be made up of water with the raw material including following parts by weight of component：30~80 parts of cotton seed hulls；Wood chip 30~80
Part；1~30 part of wheat bran；1~2 part of sucrose；0.5~3 part of gypsum；0.15~0.45 part of potassium dihydrogen phosphate；Magnesium sulfate 0.4~0.8
Part.The present invention optimizes design by constituent and content to cultivating white fungus culture medium, when ensureing that it cultivates white fungus
With higher yields and while quality, the consumption of cotton seed hulls in culture medium is reduced, the security of culture medium is improved, has
Preferable economic benefit and environmental benefit.Test result indicates that, the culture medium provided using the present invention cultivates biology during white fungus
Learn efficiency (being calculated with dry weight) and be more than 13%；The Thick many candies content for cultivating obtained white fungus is more than 20g/100g, and total sugar content is big
In 73g/100g, crude protein (fresh weight) content is more than 10g/100g.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
The invention provides a kind of cultivating white fungus culture medium, it is made up of water with the raw material including following parts by weight of component：
30~80 parts of cotton seed hulls；
30~80 parts of wood chip；
1~30 part of wheat bran；
1~2 part of sucrose；
0.5~3 part of gypsum；
0.15~0.45 part of potassium dihydrogen phosphate；
0.4~0.8 part of magnesium sulfate.
The cultivating white fungus culture medium that the present invention is provided is by water and including cotton seed hulls, wood chip, wheat bran, sucrose, gypsum, di(2-ethylhexyl)phosphate
The raw material of hydrogen potassium and magnesium sulfate is made.Wherein, cotton seed hulls is the accessory substance after cottonseed oil expression, contains abundant cellulose, wooden
The element, also partially protein such as element and nitrogen, phosphorus, potassium.In the present invention, content of the cotton seed hulls in the raw material is 30~80
Parts by weight, preferably 40~50 parts by weight.In the present invention, cotton seed hulls after wood chip and wheat bran fermentation with collectively constituting cultivating white fungus
The major ingredient of culture medium, nutrient is provided as the matrix of cultivating white fungus, and to white fungus growth.
In the present invention, wood chip is preferably white fungus preferential species of tree wood chip, such as Fagaceae wood chip, Hamamelidaceae wood chip, birch
Section's wood chip, Elaeocarpaceae wood chip, paint Ochnaceae wood chip, Juglandaceae wood chip, Araliaceae wood chip, corylaceae wood chip, pulse family wood chip, Styracaceae
One or more in wood chip, Euphorbiaceae wood chip, pulse family wood chip and Salicaceae wood chip, more preferably Chinese bandoline wood wood chip, Qinggang wood
One or more in bits, Chinese tallow tree wood chip, Chinese sumac wood chip, persimmon wood chip and tung oil tree wood chip.In the present invention, wood chip is described
Content in raw material is 30~80 parts by weight, preferably 40~50 parts by weight.In the present invention, wood chip is sent out with cotton seed hulls and wheat bran
The major ingredient of cultivating white fungus culture medium is collectively constituted after ferment, nutrient is provided as the matrix of cultivating white fungus, and to white fungus growth.
In the present invention, wheat bran is wheat wheat cortex separated during processing flour, and wheat bran contains abundant enzyme
System, protein, carbohydrate, microorganism and mineral matter etc..In the present invention, content of the wheat bran in the raw material be 1~
30 parts by weight, preferably 5~10 parts.In the present invention, wheat bran after cotton seed hulls and wood chip fermentation with collectively constituting cultivating white fungus training
The major ingredient of base is supported, nutrient is provided as the matrix of cultivating white fungus, and to white fungus growth.
In the present invention, content of the sucrose in the raw material is 1~2 parts by weight, preferably 1~1.2 parts by weight.At this
In invention, sucrose provides the carbon source that can directly utilize for culture medium.
In the present invention, content of the gypsum in the raw material is 0.5~3 parts by weight, preferably 0.8~1.1 parts by weight.
In the present invention, gypsum can stablize calcium, the sulphur nutrient composition content in the acid-base value of cultivating white fungus culture medium, increase culture medium.
In the present invention, the gypsum is preferably land plaster.
In the present invention, content of the potassium dihydrogen phosphate in the raw material is 0.15~0.45 parts by weight, preferably 0.18
~0.2 parts by weight.In the present invention, potassium dihydrogen phosphate can increase phosphorus, the Determination of Potassium in culture medium.
In the present invention, content of the magnesium sulfate in the raw material is 0.4~0.8 parts by weight, preferably 0.5~0.6 weight
Measure part.In the present invention, magnesium sulfate can increase the magnesium element content in culture medium.
In the present invention, the water content of the culture medium is preferably 60~70wt%, more preferably 62~66wt%.At this
Invent in the one embodiment provided, the water content of the culture medium is 62~65wt%；Another reality provided in the present invention
Apply in example, the water content of the culture medium is 63~66wt%.
In some embodiments of the invention, the culture medium is made up of water with the raw material including following parts by weight of component：
40~50 parts of cotton seed hulls；
40~50 parts of wood chip；
5~10 parts of wheat bran；
1~1.2 part of sucrose；
0.8~1.1 part of gypsum；
0.18~0.2 part of potassium dihydrogen phosphate；
0.5~0.6 part of magnesium sulfate.
The present invention optimizes design by constituent and content to cultivating white fungus culture medium, is ensureing its cultivation silver
While there is higher yields and quality during ear, reduce the consumption of cotton seed hulls in culture medium, not only slow down some areas and
Part season reduces the substrate-level of foreign gene drift to the cotton seed hulls situation that supply falls short of demand, to the product of cultivating white fungus
Matter and improved safety have Important Economic meaning.Test result indicates that, during the culture medium cultivation white fungus provided using the present invention
Biological efficiency (being calculated with dry weight) is more than 13%；The Thick many candies content for cultivating obtained white fungus is more than 20g/100g, and total reducing sugar contains
Amount is more than 73g/100g, and crude protein (fresh weight) content is more than 10g/100g.
Invention also provides a kind of preparation method for preparing cultivating white fungus culture medium of the present invention, including following step
Suddenly：
A), cotton seed hulls, wood chip and wheat bran are soaked in water, wet feed is obtained；
B), the wet feed is mixed with sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water, fermentation is banked up, obtains white fungus
Culture medium for cultivating.
In the preparation method that the present invention is provided, cotton seed hulls, wood chip and wheat bran are soaked in water first.The present invention's
In preferred embodiment, the cotton seed hulls, wood chip and wheat bran soak in 0.5~2wt% limewash.Wherein, the lime
The concentration of water is preferably 1~1.5wt%.Soak after finishing, obtain wet feed.In the preparation method that the present invention is provided, preferably exist
Before cotton seed hulls, wood chip and wheat bran are soaked, it is exposed to the sun, to kill the miscellaneous bacteria in material using ultraviolet.It is described sudden and violent
The time of solarization is preferably 2~3 days.
Obtain after wet feed, the wet feed is mixed with sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water, bank up fermentation.
Wherein, the time for banking up dinner party is preferably 4~6 days, more preferably 5~6 days.In the present invention, it is preferred to bank up fermentation
During every turning in 2~3 days once.In the present invention, the stockpile for banking up fermentation preferably trapezoidal heap；Stockpile height is preferably
0.5~2cm, more preferably 1~1.2cm；Wide stockpile is preferably 1~3m, more elects 2~3m as；The material dry weight of each stockpile
It is preferably controlled in 300~600kg, more preferably 350~500kg.In the present invention, it is preferably provided with passage on the stockpile
Road；The diameter of the vent cap is preferably 10~20cm, more preferably 12~15cm；The density of setting of the vent cap is excellent
Elect 1~2/m as3；The vent cap is preferably vertically arranged, by stockpile surface straight through reactor bottom.In the present invention, the fermentation
Mode be preferably spontaneous fermentation.Bank up after fermentation ends, obtain cultivating white fungus culture medium.
In one embodiment that the present invention is provided, carry out banking up fermentation in such a way：
Turning is carried out after the wet feed is banked up into fermentation 2~3 days, and adds sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate
And water, continue to bank up fermentation, obtain cultivating white fungus culture medium.
In above-described embodiment that the present invention is provided, sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water are in the wet feed
Fermentation is banked up add during first time turning.Wherein, gypsum is preferably directly added into the stockpile that wet feed banks up fermentation；Sucrose,
Potassium dihydrogen phosphate and magnesium sulfate are then preferred to be first mixed to get solution with a certain amount of water, then the solution is added into wet feed banks up hair
In the stockpile of ferment.Add after sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water, proceed to bank up fermentation.Continuing to bank up
In fermentation process, preferably every turning in 2~3 days is once.In above-described embodiment that the present invention is provided, the total time for banking up fermentation is excellent
Elect as 4~6 days, bank up after fermentation ends, obtain cultivating white fungus culture medium.
In the present invention, the consumption of water is not particularly limited, its specific consumption regards the aqueous of final obtained culture medium
Depending on amount.In the present invention, the water content that cultivating white fungus culture medium is obtained after fermentation ends is preferably controlled in as 60~70wt%,
More preferably 62~66wt%.
The culture medium prepared using the method that provides of the present invention have when ensureing that it cultivates white fungus higher yields and
While quality, the consumption of cotton seed hulls in culture medium is reduced, some areas and part season is not only slow down to cotton seed hulls confession
The situation that should not be asked, and the substrate-level of foreign gene drift is reduced, there is weight to the quality and improved safety of cultivating white fungus
Want economic implications.Test result indicates that, the biological efficiency during culture medium cultivation white fungus for using the present invention to prepare is (in terms of dry weight
Calculate) it is more than 13%；The Thick many candies content for cultivating obtained white fungus is more than 20g/100g, and total sugar content is more than 73g/100g, thick egg
(fresh weight) content is more than 10g/100g in vain.
For the sake of becoming apparent from, it is described in detail below by following examples.
Embodiment 1
A kind of cultivating white fungus culture medium, is made up of the raw material components of water and following weight parts：40 parts of cotton seed hulls, wood shavings nanmu
0.6 part of 40 parts of bits, 10 parts of wheat bran, 1 part of sucrose, 1.1 parts of land plaster, 0.2 part of potassium dihydrogen phosphate and magnesium sulfate；
Preparation method：
1) material is shone：Cotton seed hulls, wood chip and wheat bran are exposed to the sun 2 days before spice, it is miscellaneous to kill part in raw material using ultraviolet
Bacterium；
2) prewet：Cotton seed hulls, wood chip and wheat bran are turned uniformly in the dry state, then entered with 1wt% lime aqueous
Row is prewetted, fully drenched, obtains wet feed；
3) fermentation is banked up：Wet feed is piled into high 1.2cm, wide 3m, the trapezoidal heap that length is not limited, the planting material dry weight per heap
500kg, is carried out after heap, and surrounding is patted, then some vertical stomatas, straight through reactor bottom, gas are inserted on heap with diameter 15cm wooden stick
Hole density is 1/m3, finally cover grass and allow its spontaneous fermentation；
4) turning：Bank up the 2nd day and the 4th day after fermentation and carry out turning, the material on side is ploughed under center during each turning.
Land plaster, sucrose, potassium dihydrogen phosphate and magnesium sulfate are added during first time turning, wherein, gypsum is directly added into wet feed and banks up fermentation
Stockpile in；Sucrose, potassium dihydrogen phosphate and magnesium sulfate are then first mixed to get solution with a certain amount of water, then the solution is added to
Wet feed is banked up in the stockpile of fermentation.Water content depending on windrow keeps the skin wet, after banking up fermentation 5 days, terminates fermentation, obtains water content
For 62~65wt% cultivating white fungus culture medium.
Embodiment 2
A kind of cultivating white fungus culture medium, is made up of the raw material components of water and following weight parts：50 parts of cotton seed hulls, wood shavings nanmu
0.5 part of 50 parts of bits, 5 parts of wheat bran, 1.2 parts of sucrose, 0.8 part of land plaster, 0.18 part of potassium dihydrogen phosphate and magnesium sulfate；
Preparation method：
1) material is shone：Cotton seed hulls, wood chip and wheat bran are exposed to the sun 2 days before spice, it is miscellaneous to kill part in raw material using ultraviolet
Bacterium；
2) prewet：Cotton seed hulls, wood chip and wheat bran are turned uniformly in the dry state, then entered with 1wt% lime aqueous
Row is prewetted, fully drenched, obtains wet feed；
3) fermentation is banked up：Wet feed is piled into high 1cm, wide 2m, the trapezoidal heap that length is not limited, the planting material dry weight per heap
350kg, is carried out after heap, and surrounding is patted, then some vertical stomatas, straight through reactor bottom, gas are inserted on heap with diameter 12cm wooden stick
Hole density is 1/m3, finally cover grass and allow its spontaneous fermentation；
4) turning：Bank up the 2nd day and the 4th day after fermentation and carry out turning, the material on side is ploughed under center during each turning.
Land plaster, sucrose, potassium dihydrogen phosphate and magnesium sulfate are added during first time turning, wherein, gypsum is directly added into wet feed and banks up fermentation
Stockpile in；Sucrose, potassium dihydrogen phosphate and magnesium sulfate are then first mixed to get solution with a certain amount of water, then the solution is added to
Wet feed is banked up in the stockpile of fermentation.Water content depending on windrow keeps the skin wet, after banking up fermentation 5 days, terminates fermentation, obtains water content
For 63~66wt% cultivating white fungus culture medium.
Embodiment 3
A kind of cultivating white fungus culture medium, is made up of the raw material components of water and following weight parts：45 parts of cotton seed hulls, wood shavings nanmu
0.6 part of 45 parts of bits, 8 parts of wheat bran, 2 parts of sucrose, 1.1 parts of land plaster, 0.2 part of potassium dihydrogen phosphate and magnesium sulfate；
Preparation method：
1) material is shone：Cotton seed hulls, wood chip and wheat bran are exposed to the sun 2 days before spice, it is miscellaneous to kill part in raw material using ultraviolet
Bacterium；
2) prewet：Cotton seed hulls, wood chip and wheat bran are turned uniformly in the dry state, then entered with 2wt% lime aqueous
Row is prewetted, fully drenched, obtains wet feed；
3) fermentation is banked up：Wet feed is piled into high 1.2cm, wide 3m, the trapezoidal heap that length is not limited, the planting material dry weight per heap
500kg, is carried out after heap, and surrounding is patted, then some vertical stomatas, straight through reactor bottom, gas are inserted on heap with diameter 15cm wooden stick
Hole density is 2/m3, finally cover grass and allow its spontaneous fermentation；
4) turning：Bank up the 2nd day and the 4th day after fermentation and carry out turning, the material on side is ploughed under center during each turning.
Land plaster, sucrose, potassium dihydrogen phosphate and magnesium sulfate are added during first time turning, wherein, gypsum is directly added into wet feed and banks up fermentation
Stockpile in；Sucrose, potassium dihydrogen phosphate and magnesium sulfate are then first mixed to get solution with a certain amount of water, then the solution is added to
Wet feed is banked up in the stockpile of fermentation.Water content depending on windrow keeps the skin wet, after banking up fermentation 6 days, terminates fermentation, obtains water content
For 62~65wt% cultivating white fungus culture medium.
Embodiment 4
A kind of cultivating white fungus culture medium, is made up of the raw material components of water and following weight parts：30 parts of cotton seed hulls, wood shavings nanmu
0.8 part of 80 parts of bits, 30 parts of wheat bran, 2 parts of sucrose, 3 parts of land plaster, 0.45 part of potassium dihydrogen phosphate and magnesium sulfate；
Preparation method：
1) material is shone：Cotton seed hulls, wood chip and wheat bran are exposed to the sun 2 days before spice, it is miscellaneous to kill part in raw material using ultraviolet
Bacterium；
2) prewet：Cotton seed hulls, wood chip and wheat bran are turned uniformly in the dry state, then with 0.5wt% lime aqueous
Prewetted, it is fully drenched, obtain wet feed；
3) fermentation is banked up：Wet feed is piled into high 1.1cm, wide 2.5m, the trapezoidal heap that length is not limited, the planting material dry weight per heap
500kg, is carried out after heap, and surrounding is patted, then some vertical stomatas, straight through reactor bottom, gas are inserted on heap with diameter 15cm wooden stick
Hole density is 2/m3, finally cover grass and allow its spontaneous fermentation；
4) turning：Bank up the 2nd day and the 4th day after fermentation and carry out turning, the material on side is ploughed under center during each turning.
Land plaster, sucrose, potassium dihydrogen phosphate and magnesium sulfate are added during first time turning, wherein, gypsum is directly added into wet feed and banks up fermentation
Stockpile in；Sucrose, potassium dihydrogen phosphate and magnesium sulfate are then first mixed to get solution with a certain amount of water, then the solution is added to
Wet feed is banked up in the stockpile of fermentation.Water content depending on windrow keeps the skin wet, after banking up fermentation 6 days, terminates fermentation, obtains water content
For 62~65wt% cultivating white fungus culture medium.
Embodiment 5
A kind of cultivating white fungus culture medium, is made up of the raw material components of water and following weight parts：80 parts of cotton seed hulls, wood shavings nanmu
0.4 part of 30 parts of bits, 3 parts of wheat bran, 1.5 parts of sucrose, 0.5 part of land plaster, 0.15 part of potassium dihydrogen phosphate and magnesium sulfate；
Preparation method：
1) material is shone：Cotton seed hulls, wood chip and wheat bran are exposed to the sun 2 days before spice, it is miscellaneous to kill part in raw material using ultraviolet
Bacterium；
2) prewet：Cotton seed hulls, wood chip and wheat bran are turned uniformly in the dry state, then with 1.5wt% lime aqueous
Prewetted, it is fully drenched, obtain wet feed；
3) fermentation is banked up：Wet feed is piled into high 1.2cm, wide 3m, the trapezoidal heap that length is not limited, the planting material dry weight per heap
500kg, is carried out after heap, and surrounding is patted, then some vertical stomatas, straight through reactor bottom, gas are inserted on heap with diameter 15cm wooden stick
Hole density is 2/m3, finally cover grass and allow its spontaneous fermentation；
4) turning：Bank up the 2nd day and the 4th day after fermentation and carry out turning, the material on side is ploughed under center during each turning.
Land plaster, sucrose, potassium dihydrogen phosphate and magnesium sulfate are added during first time turning, wherein, gypsum is directly added into wet feed and banks up fermentation
Stockpile in；Sucrose, potassium dihydrogen phosphate and magnesium sulfate are then first mixed to get solution with a certain amount of water, then the solution is added to
Wet feed is banked up in the stockpile of fermentation.Water content depending on windrow keeps the skin wet, after banking up fermentation 6 days, terminates fermentation, obtains water content
For 62~65wt% cultivating white fungus culture medium.
Embodiment 6
Culture medium effect assessment
By the embodiment of the present application 1~3 prepare culture medium and GB/T29369-2012 formula one (cotton seed hulls 88wt%,
Wheat bran 11wt%, land plaster 1wt%, 55~60wt% of water content) and two (wood chip 60wt%, soybean straw 23wt%, wheat brans of formula
15wt%, land plaster 2wt%, 55~60wt% of water content) carry out cultivating white fungus contrast test, the concrete operation step of cultivation and
Condition is as follows：
1) pocket processed：
1-1) pack
With semi-automatic spiral pressing material type charger filler, compost is filled into from sack 8cm or so.Each pocket fills out wet feed
1.1kg~1.2kg, each 500 bags of formula system.
1-2) prick sack
Prick sack when, pocket is holded up, compress charge level, by inside and outside sack two sides adhere to compost erasing it is clean after, bag
Mouth is threaded together, and presses close to charge level, reflexed after being enclosed with cotton thread colligation 3, then pricks two circles again, tenses the end of a thread.
1-3) beat cave
The pocket for tying mouth is slightly flattened with plank, then cave is beaten in pocket one side with diameter 1.2cm point diggers or wooden stick,
Every bag is made a call to 3~4 equidistant caves, pocket diameter 1.2cm, deep 2cm, and the impurity on bag surface that uses cloth to wipe with.
1-4) rubberized fabric
Pocket is sealed with 3.3cm × 3.3cm Special adhesive tape for edible mushroom, pocket surrounding obturages densification.
2) pocket sterilizes：
2-1) make a fire heating
Temperature will reach 100 DEG C in pot storehouse in 3.5~4h, then keep 100 DEG C to reach 22h.
2-2) unload pot
Reach after sterilization time, cease fire, temperature is dropped to 80 DEG C or so the coolings that can take the dish out of the pot.Cooling place needs clear in advance
Wash clean, and with formalin-potassium permanganate fumigation 24h, open window and distribute after formalin, the pocket after sterilizing is removed
Enter cooling chamber, ' well ' font stacking.If it was found that pocket adhesive plaster is tilted or broken bag, should be sealed immediately with adhesive plaster, with antiforeign bacteria
Intrusion.Carrying tool needs one layer of gunnysack of pad, to prevent puncturing polybag.
3) it is inoculated with：
3-1) dress seed
15h is dressed seed before inoculation.Select 8~10d of cell age three-level kind.After inoculating hood sterilization, pull out and abandon tampon, first with connecing
The white fungus former base on kind of hilt strain top layer is dug and abandoned, and top layer 2cm or so is smashed to pieces, then the more loose incense ashes subiculum 4 of lower floor~
6cm, which has been dug, to be mixed with uniformly.Plastic cloth after being sterilized with block once seals bottleneck, and rubber band is tightened.Because white fungus bacterium
It is that, by two kinds of bacterium mass seed productions, white fungus mycelia is only grow on culture medium top layer 2cm or so, and mycelia is fine and close, solid, incense ashes mycelia to plant
Then whole bottle culture medium has.
3-2) transfer room is sterilized again
Aerosol disinfection box is sterilized：Consumption is calculated by every cubic metre of space 4g~5g, disinfection box Chinese medicine is lighted and produces gas
Mist is sterilized, and transfer room answers closed more than 30min.
3-3) inoculation temperature and time
Unload the pocket temperature being emitted on after pot in transfer room to wait to drop to 28~30 DEG C, that is, be inoculated with.
4) bacterium germination：
Bacterium germination is carried out according to the planting type of table 1
The cultivation step and specific cultivation condition of the white fungus of table 1
After cultivation terminates, collection and composition detection, method, the condition of composition detection of white fungus are carried out, or reference standard is such as
Under：
1) total reducing sugar
Total reducing sugar：Performed by GB/T 15672 regulation.
2) Thick many candies
2-1) dry：5 samples are in heated-air drying 12h at 90 DEG C, with equilibrium water conten；
2-2) crush：Universalpulverizer is crushed, and crosses 40 mesh sieves (sieve aperture is 380 microns)；
2-3) extract：Liquid ratio 60:1 (v/w), 90 DEG C of Extracting temperature, extraction time 4h, solvent is the water-soluble of pH neutrality
Liquid；
2-4) determine：Performed as defined in NY/T1676-2008.
3) crude protein
Crude protein is detected：Performed by as defined in GB/T 15673.
As shown in table 2, table 2 is the nutritional ingredient data and biology effect of different culture media cultivation white fungus to composition detection result
Rate.
The nutritional ingredient data and biological efficiency of the different culture media of table 2 cultivation white fungus
It can be seen from Table 2 that, when the culture medium provided using the present invention cultivates white fungus, per kilogram Tremella cultivation medium harvest
White fungus amount is nearly 1.5 times of the cultivation base yield of formula one, is that the cultivation base yield of formula two produces nearly 2 times, and white fungus Thick many candies ratio
Formula one is higher by nearly 5%, and nearly 8%, rich in nutrition content, with good planting benefit are higher by than formula two.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of cultivating white fungus culture medium, is made up of water with the raw material including following parts by weight of component：
2. culture medium according to claim 1, it is characterised in that the culture medium is by water and including following parts by weight of component
Raw material be made：
3. culture medium according to claim 1, it is characterised in that the water content of the culture medium is 62~66wt%.
4. according to any described culture medium of claims 1 to 3, its preparation method comprises the following steps：
A), cotton seed hulls, wood chip and wheat bran are soaked in water, wet feed is obtained；
B), the wet feed is mixed with sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water, fermentation is banked up, obtains cultivating white fungus
Culture medium.
5. preparation method according to claim 4, it is characterised in that the step a) is：
Cotton seed hulls, wood chip and wheat bran soak in 0.5~2wt% limewash, obtain wet feed.
6. preparation method according to claim 4, it is characterised in that in step b), the time for banking up fermentation is 4~
6 days.
7. preparation method according to claim 6, it is characterised in that in step b), it is described bank up every 2 in fermentation process~
Turning in 3 days is once.
8. preparation method according to claim 4, it is characterised in that the step b) is：
Carry out turning after the wet feed is banked up into fermentation 2~3 days, and add sucrose, gypsum, potassium dihydrogen phosphate, magnesium sulfate and water,
Continue to bank up fermentation, obtain cultivating white fungus culture medium.
9. preparation method according to claim 4, it is characterised in that the stockpile for banking up fermentation is trapezoidal heap, stockpile
A height of 1~1.2cm, a width of 2~3m of stockpile.
10. preparation method according to claim 9, it is characterised in that be provided with vent cap on the stockpile；It is described logical
A diameter of 12~15cm in stomata road；The density of setting of the vent cap is 1~2/m3。
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006180A (en) * | 2018-08-23 | 2018-12-18 | 安徽农耕年华农业发展有限公司 | A kind of mushroom culture medium and preparation method thereof |
| CN109892170A (en) * | 2019-04-26 | 2019-06-18 | 悦田坊生态农业(婺源县)有限公司 | Novel herbal tremella culture medium of one kind and preparation method thereof |
| CN110896785A (en) * | 2019-12-05 | 2020-03-24 | 山东安华生物医药股份有限公司 | Method for cultivating tremella fuciformis strains |
| CN112840946A (en) * | 2020-12-31 | 2021-05-28 | 安徽丰原发酵技术工程研究有限公司 | Golden fungus culture medium and method for culturing golden fungus by using same |
| CN113711850A (en) * | 2021-09-18 | 2021-11-30 | 四川工大西南食品研究有限责任公司 | Tremella fuciformis cultivation method, tremella fuciformis, culture medium and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580804A (en) * | 2008-05-12 | 2009-11-18 | 黄刚 | Preparation method of tremella culture material |
| CN102696401A (en) * | 2012-07-03 | 2012-10-03 | 韶关学院 | Factorial method for cultivating white fungi |
| CN104609984A (en) * | 2015-02-08 | 2015-05-13 | 邬金梅 | Preparation method of tremella cultivation material |
-
2016
- 2016-04-26 CN CN201610266600.1A patent/CN107311732A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580804A (en) * | 2008-05-12 | 2009-11-18 | 黄刚 | Preparation method of tremella culture material |
| CN102696401A (en) * | 2012-07-03 | 2012-10-03 | 韶关学院 | Factorial method for cultivating white fungi |
| CN104609984A (en) * | 2015-02-08 | 2015-05-13 | 邬金梅 | Preparation method of tremella cultivation material |
Non-Patent Citations (1)
| Title |
|---|
| 潘洪玉等: "《珍稀食用菌栽培技术》", 31 March 2010, 吉林科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006180A (en) * | 2018-08-23 | 2018-12-18 | 安徽农耕年华农业发展有限公司 | A kind of mushroom culture medium and preparation method thereof |
| CN109892170A (en) * | 2019-04-26 | 2019-06-18 | 悦田坊生态农业(婺源县)有限公司 | Novel herbal tremella culture medium of one kind and preparation method thereof |
| CN110896785A (en) * | 2019-12-05 | 2020-03-24 | 山东安华生物医药股份有限公司 | Method for cultivating tremella fuciformis strains |
| CN112840946A (en) * | 2020-12-31 | 2021-05-28 | 安徽丰原发酵技术工程研究有限公司 | Golden fungus culture medium and method for culturing golden fungus by using same |
| CN113711850A (en) * | 2021-09-18 | 2021-11-30 | 四川工大西南食品研究有限责任公司 | Tremella fuciformis cultivation method, tremella fuciformis, culture medium and application |
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