CN112840946A - Golden fungus culture medium and method for culturing golden fungus by using same - Google Patents
Golden fungus culture medium and method for culturing golden fungus by using same Download PDFInfo
- Publication number
- CN112840946A CN112840946A CN202011642183.9A CN202011642183A CN112840946A CN 112840946 A CN112840946 A CN 112840946A CN 202011642183 A CN202011642183 A CN 202011642183A CN 112840946 A CN112840946 A CN 112840946A
- Authority
- CN
- China
- Prior art keywords
- golden
- culture medium
- lactate
- fungus
- golden fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 78
- 239000001963 growth medium Substances 0.000 title claims abstract description 42
- 238000012258 culturing Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 238000012136 culture method Methods 0.000 claims abstract description 5
- 241000117280 Naematelia aurantialba Species 0.000 claims description 30
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 19
- 229940001447 lactate Drugs 0.000 claims description 19
- 235000012343 cottonseed oil Nutrition 0.000 claims description 12
- 239000010440 gypsum Substances 0.000 claims description 11
- 229910052602 gypsum Inorganic materials 0.000 claims description 11
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 11
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 11
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 9
- 239000001527 calcium lactate Substances 0.000 claims description 9
- 235000011086 calcium lactate Nutrition 0.000 claims description 9
- 229960002401 calcium lactate Drugs 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 4
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 3
- PHZLMBHDXVLRIX-UHFFFAOYSA-M potassium lactate Chemical compound [K+].CC(O)C([O-])=O PHZLMBHDXVLRIX-UHFFFAOYSA-M 0.000 claims description 3
- 239000001521 potassium lactate Substances 0.000 claims description 3
- 235000011085 potassium lactate Nutrition 0.000 claims description 3
- 229960001304 potassium lactate Drugs 0.000 claims description 3
- 239000001540 sodium lactate Substances 0.000 claims description 3
- 235000011088 sodium lactate Nutrition 0.000 claims description 3
- 229940005581 sodium lactate Drugs 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 230000002458 infectious effect Effects 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 description 7
- 241001506047 Tremella Species 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001505683 Tremella aurantia Species 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000221424 Tremellaceae Species 0.000 description 1
- 241000221365 Tremellales Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a golden fungus culture medium and a method for culturing golden fungus by using the same. The golden fungus culture medium provided by the invention is used for culturing and producing golden fungus, and can effectively prevent the golden fungus from being infected with infectious microbes in the culture process, so that the fruiting rate of the golden fungus is improved, the culture cost of the golden fungus is reduced, and the golden fungus culture medium provided by the invention has the advantages of simple and easily obtained raw materials and simple operation of the culture method.
Description
Technical Field
The invention belongs to the technical field of golden fungus planting, and relates to a golden fungus culture medium and a method for culturing golden fungus by using the same.
Background
The Tremella aurantia is named Tremella aurantia and belongs to Tremellales, Tremellaceae and Tremella in the fungus classification; because of their golden color, they are also called golden fungus, golden tremella and golden tremella, and also called brain fungus and brain-shaped tremella because they are similar to human brain.
The tremella aurantialba is rich in protein, amino acids essential to human bodies, multiple vitamins, tremella aurantialba polysaccharide and trace elements such as manganese, iron, magnesium, calcium, potassium and the like, is a nutritional tonic, can be eaten or used as a medicine, is a rare edible fungus used as both food and medicine, has a nutritional value superior to that of colloidal fungi such as tremella, black fungus and the like, and is an ideal high-grade delicacy and health care product.
China successfully explores the artificial cultivation technology of the tremella aurantialba from the 80 th of the last century, starts to popularize nationwide, develops over twenty years, and is mature along with the increase of market demand by the large-scale cultivation technology of the tremella aurantialba by double-bacterium composite inoculation and bag cultivation. However, in the actual production process of tremella aurantialba, due to the growth characteristics of tremella aurantialba, the bag material is very easy to be infected with sundry fungi in the early fruiting stage, the reproduction of the sundry fungi not only affects the fruiting rate, but also generates a large amount of spores which are diffused into the air in the carrying process of fungus bags, so that the infection risk of other healthy fungus bags is further increased, and the problem restricts the large-scale factory cultivation of the tremella aurantialba.
Therefore, it is desirable to provide a culture medium that can reduce the risk of contaminating microbes during the cultivation of Tremella aurantialba.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the golden fungus culture medium and the method for culturing the golden fungus by using the golden fungus culture medium.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a golden fungus culture medium, wherein the raw materials of the golden fungus culture medium comprise lactate.
According to the invention, lactate is added into the golden fungus culture medium, so that the probability of infecting mixed bacteria in the cultivation process of golden fungus can be effectively reduced, and the fruiting rate is improved.
In order to reduce the contamination of infectious microbes without affecting the inoculation and subsequent growth of the golden fungus, the addition amount of the lactate is limited, and the addition amount of the lactate is 0.5-1%, such as 0.6%, 0.7%, 0.8%, 0.9% and the like, and is preferably 1% based on the total mass of the golden fungus culture medium as 100%.
If the amount of lactate added is low, the objective of reducing the contamination rate cannot be achieved, and if the amount of lactate added is high, the pH of the tremella aurantialba culture medium is excessively reduced, which is disadvantageous to the cultivation of tremella aurantialba.
In a preferred embodiment of the present invention, the lactate is selected from any one of calcium lactate, sodium lactate, and potassium lactate, or a combination of at least two thereof.
In the present invention, the other components except lactate in the tremella aurantialba culture medium can be the existing conventional components, and the other components in the tremella aurantialba culture medium preferably in the present invention include cottonseed hulls, wood flour, bran, monopotassium phosphate, gypsum and lactate.
As a preferred technical scheme of the invention, the golden fungus culture medium comprises 30-50 parts by weight of cottonseed hulls, 30-50 parts by weight of sawdust, 10-20 parts by weight of bran, 1-5 parts by weight of monopotassium phosphate, 1-5 parts by weight of gypsum and 0.5-1 part by weight of lactate.
As a preferable technical scheme of the invention, the golden fungus culture medium consists of 30-50% of cottonseed hulls, 30-50% of wood chips, 10-20% of bran, 1-5% of monopotassium phosphate, 1-5% of gypsum and 0.5-1% of lactate by taking the total mass of the golden fungus culture medium as 100%.
As a specific embodiment of the invention, the golden fungus culture medium consists of 41% of cottonseed hulls, 42% of wood chips, 14% of bran, 1% of monopotassium phosphate, 1% of gypsum and 1% of lactate, wherein the total mass of the golden fungus culture medium is 100%.
In a second aspect, the present invention provides a method for culturing tremella aurantialba, comprising culturing tremella aurantialba using the medium for tremella aurantialba according to the first aspect.
The culture method comprises the following steps: and (2) preparing a tremella aurantialba fungus bag by using the tremella aurantialba culture medium of the first aspect, and then carrying out inoculation culture to obtain the tremella aurantialba.
The inoculation, culture and the like of the invention are all conventional methods at present.
The culture method of the invention also comprises the step of sterilizing the fungus bags before inoculation, preferably sterilizing the fungus bags at the temperature of 120-125 ℃ for 60-70 min.
Compared with the prior art, the invention has the following beneficial effects:
the golden fungus culture medium provided by the invention can be used for culturing and producing golden fungus, can effectively prevent the golden fungus from being infected with infectious microbes in the culture process, improves the fruiting rate of the golden fungus, and reduces the culture cost of the golden fungus, and the golden fungus culture medium provided by the invention can adjust the basic acidity and alkalinity of culture on the premise of not remarkably increasing the cost of raw materials, so that the golden fungus culture medium is more suitable for the growth of the golden fungus, the raw materials are simple and easy to obtain, and the culture method is simple to operate.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the specific embodiments are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
The embodiment provides a golden fungus culture medium and a method for culturing golden fungus by using the same.
(1) Preparing a tremella aurantialba bag: mixing cottonseed hull 41.5%, sawdust 42%, bran 14%, potassium dihydrogen phosphate 1%, gypsum 1%, and 0.5% calcium lactate, stirring, bagging in 1000 bags, and sterilizing at 121 deg.C for 60 min;
(2) inoculating and culturing: inoculating the cooled fungus bag in a sterile environment, performing static culture in a suitable environment after inoculation, and opening two areas with the area of about 10cm on the fungus bag along the longitudinal axis of the fungus bag by using a sterile tool when the mushroom bag begins to grow2The distance between the two openings is about 7-8 cm, then the cultivation is continued until mature sporocarp grows out, and the fruiting rate and the conversion rate are calculated after picking.
Examples 2 to 4
The embodiment provides a golden fungus culture medium and a method for culturing golden fungus by using the same.
The difference from example 1 is that in the golden ear medium provided in this example, 0.5% calcium lactate was replaced with 1% calcium lactate (example 2), 1% sodium lactate (example 3), and 1% potassium lactate (example 4), with a corresponding reduction in the amount of cottonseed hulls added of 41%.
Example 5
The embodiment provides a golden fungus culture medium and a method for culturing golden fungus by using the same.
The difference from example 1 is that the amount of calcium lactate added to the golden ear medium provided in this example was 2%.
Example 6
The embodiment provides a golden fungus culture medium and a method for culturing golden fungus by using the same.
(1) Preparing a tremella aurantialba bag: fully stirring and mixing 30% of cottonseed hulls, 50% of sawdust, 10% of bran, 4% of potassium dihydrogen phosphate, 5% of gypsum and 1% of calcium lactate, bagging in 1000 bags, and sterilizing for 60 minutes at 121 ℃;
(2) inoculating and culturing: inoculating the cooled fungus bag in a sterile environment, performing static culture in a suitable environment after inoculation, and opening two areas with the area of about 10cm on the fungus bag along the longitudinal axis of the fungus bag by using a sterile tool when the mushroom bag begins to grow2The distance between the two openings is about 7-8 cm, then the cultivation is continued until mature sporocarp grows out, and the fruiting rate and the conversion rate are calculated after picking.
Example 7
The embodiment provides a golden fungus culture medium and a method for culturing golden fungus by using the same.
(1) Preparing a tremella aurantialba bag: fully stirring and mixing 50% of cottonseed hulls, 30% of sawdust, 16% of bran, 1% of potassium dihydrogen phosphate, 2% of gypsum and 1% of calcium lactate, bagging in 1000 bags, and sterilizing for 60 minutes at 121 ℃;
(2) inoculating and culturing: inoculating the cooled fungus bag in a sterile environment, performing static culture in a suitable environment after inoculation, and opening two areas with the area of about 10cm on the fungus bag along the longitudinal axis of the fungus bag by using a sterile tool when the mushroom bag begins to grow2The distance between the two openings is about 7-8 cm, then the cultivation is continued until mature sporocarp grows out, and the fruiting rate and the conversion rate are calculated after picking.
Comparative example 1
The comparative example provides a tremella aurantialba culture medium and a method for culturing tremella aurantialba by using the same.
The difference from example 1 is that the culture medium for golden ears provided in this comparative example was not supplemented with calcium lactate, and four replicates were performed using the culture medium for golden ears of comparative example 1.
Performance testing
The fruiting rate, the conversion rate and the contamination rate of the tremella aurantialba of examples 1-7 and comparative example 1 are calculated as follows:
(1) and (3) fruiting rate:
in the formula, AcThe number of the collected qualified golden fungus sporocarp is AkThe maximum number of the golden fungus sporocarp which should be harvested theoretically is produced;
(2) conversion rate:
in the formula, MGM is the total weight of the harvested qualified Tremella Aurantialba fruiting bodiesLTo produce the total weight of raw materials actually filled into the fungus bags;
the results are shown in Table 1:
TABLE 1
As can be seen from the data in Table 1, the golden fungus culture medium can effectively prevent golden fungus from infecting infectious microbes in the culture process, reduce the infection rate, improve the fruiting rate and improve the conversion rate, thereby reducing the culture cost of the golden fungus; wherein, the contamination rate is below 1.6%, the fruiting rate is above 79%, and the conversion rate is above 60%.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. The golden fungus culture medium is characterized in that the raw materials of the golden fungus culture medium comprise lactate.
2. The golden ear medium according to claim 1, characterized in that the lactate is added in an amount of 0.5-1%, preferably 1%, based on 100% of the total mass of the golden ear medium.
3. A golden ear culture according to claim 1 or 2, characterized in that the lactate is selected from any one or a combination of at least two of calcium lactate, sodium lactate or potassium lactate.
4. The golden ear medium according to any one of claims 1-3, characterized in that the other components in the golden ear medium comprise cottonseed hulls, wood flour, bran, monopotassium phosphate, gypsum and lactate.
5. The golden fungus culture medium according to claim 4, wherein the raw materials of the golden fungus culture medium comprise 30-50 parts by weight of cottonseed hulls, 30-50 parts by weight of sawdust, 10-20 parts by weight of bran, 1-5 parts by weight of monopotassium phosphate, 1-5 parts by weight of gypsum and 0.5-1 part by weight of lactate.
6. The golden ear culture medium according to claim 4 or 5, which consists of 30-50% of cottonseed hulls, 30-50% of wood flour, 10-20% of bran, 1-5% of monopotassium phosphate, 1-5% of gypsum and 0.5-1% of lactate by taking the total mass of the golden ear culture medium as 100%.
7. The golden ear medium according to claim 6, which consists of 41% cottonseed hulls, 42% wood flour, 14% bran, 1% monopotassium phosphate, 1% gypsum and 1% lactate by weight of 100% total mass of the golden ear medium.
8. A method for culturing Tremella aurantialba, comprising culturing Tremella aurantialba with the culture medium according to any one of claims 1 to 7.
9. The culture method according to claim 8, comprising: a bag of Tremella aurantialba fungi is prepared by using the Tremella aurantialba culture medium of any one of claims 1 to 7, and then inoculated culture is carried out to obtain Tremella aurantialba.
10. The method according to claim 9, further comprising sterilizing the bag before inoculation, preferably at 125 ℃ for 60-70min at 120-.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011642183.9A CN112840946A (en) | 2020-12-31 | 2020-12-31 | Golden fungus culture medium and method for culturing golden fungus by using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011642183.9A CN112840946A (en) | 2020-12-31 | 2020-12-31 | Golden fungus culture medium and method for culturing golden fungus by using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112840946A true CN112840946A (en) | 2021-05-28 |
Family
ID=76000783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011642183.9A Pending CN112840946A (en) | 2020-12-31 | 2020-12-31 | Golden fungus culture medium and method for culturing golden fungus by using same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112840946A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001327220A (en) * | 1991-09-24 | 2001-11-27 | Takara Shuzo Co Ltd | Method for artificiallly cultivating lyophyllum decastes |
| CN101603000A (en) * | 2009-07-16 | 2009-12-16 | 天津科技大学 | A kind of preparation method who is rich in γ-An Jidingsuan vinegar |
| CN106367353A (en) * | 2016-08-25 | 2017-02-01 | 四川省大真科技有限责任公司 | Culture medium and tremella fuciformis strain prepared by using same |
| CN107311732A (en) * | 2016-04-26 | 2017-11-03 | 无限极（中国）有限公司 | A kind of cultivating white fungus culture medium and preparation method thereof |
-
2020
- 2020-12-31 CN CN202011642183.9A patent/CN112840946A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001327220A (en) * | 1991-09-24 | 2001-11-27 | Takara Shuzo Co Ltd | Method for artificiallly cultivating lyophyllum decastes |
| CN101603000A (en) * | 2009-07-16 | 2009-12-16 | 天津科技大学 | A kind of preparation method who is rich in γ-An Jidingsuan vinegar |
| CN107311732A (en) * | 2016-04-26 | 2017-11-03 | 无限极（中国）有限公司 | A kind of cultivating white fungus culture medium and preparation method thereof |
| CN106367353A (en) * | 2016-08-25 | 2017-02-01 | 四川省大真科技有限责任公司 | Culture medium and tremella fuciformis strain prepared by using same |
Non-Patent Citations (2)
| Title |
|---|
| 刘平、汪欣: "烘焙食品防腐保鲜常见问题及解答", vol. 1, 上海交通大学出版社, pages: 97 - 69 * |
| 王玉珏: "凝结芽胞杆菌利用非粮生物质合成乳酸的研究", no. 08 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102498937B (en) | Method for culturing oyster mushroom | |
| CN111713335B (en) | Efficient sparassis crispa cultivation method | |
| CN102964170A (en) | Formula and preparation method of agaricus bisporus liquid spawn | |
| CN113455286B (en) | Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible fungi | |
| CN108522147A (en) | The culture medium and cultural method of economical short cycle cultivation Pleurotus eryngii | |
| CN106431658B (en) | Oyster mushroom culture medium and oyster mushroom cultivation method | |
| CN112840946A (en) | Golden fungus culture medium and method for culturing golden fungus by using same | |
| CN108901611A (en) | A kind of Antrodia camphorata culture medium and its preparation method and application | |
| CN114402913A (en) | Pleurotus eryngii culture medium and preparation method of original mother seeds | |
| CN111264303B (en) | Lyophyllum fumosoroseum cultivar and preparation method thereof | |
| CN114431072A (en) | Method for breeding shiitake mushrooms through ARTP (active ARTP) plasma mutagenesis | |
| CN111615995A (en) | Culture medium for liquid fermentation of pleurotus eryngii | |
| CN109076881B (en) | Culture method and application of selenium-rich hericium erinaceus mycelium | |
| CN111642326A (en) | Mother culture method of pleurotus eryngii liquid strain | |
| CN111869506A (en) | Production method of black-skin termitomyces albuminosus bag | |
| CN117178809A (en) | Tremella aurantialba culture medium and application thereof | |
| CN109247189A (en) | A kind of white ginseng bacterial strain and its white ginseng low cost cultural method | |
| CN110129205A (en) | A kind of Pleurotus nebrodensis liquid spawn culture medium and Pleurotus nebrodensis liquid spawn preparation method | |
| CN116569786B (en) | Preparation method of edible fungus solid mycelium capable of being directly eaten | |
| CN114342736B (en) | Standardized production process of lentinus edodes stick | |
| CN117178808A (en) | Needle mushroom culture medium and application thereof | |
| JPH01291726A (en) | Artificial culture of mushroom | |
| CN116590157A (en) | Formula and preparation method of liquid strain for producing stropharia rugoso-annulata | |
| CN106748118A (en) | A kind of preparation method of good blue tissue-cultured derived plant lily transplanting fertilizer | |
| CN116420550A (en) | Culture method of trametes robiniophila |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210528 |