JPH01291726A - Artificial culture of mushroom - Google Patents
Artificial culture of mushroomInfo
- Publication number
- JPH01291726A JPH01291726A JP63119376A JP11937688A JPH01291726A JP H01291726 A JPH01291726 A JP H01291726A JP 63119376 A JP63119376 A JP 63119376A JP 11937688 A JP11937688 A JP 11937688A JP H01291726 A JPH01291726 A JP H01291726A
- Authority
- JP
- Japan
- Prior art keywords
- mushrooms
- culture medium
- sawdust
- culture
- mushroom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 240000000599 Lentinula edodes Species 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 11
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims abstract description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 9
- 235000009566 rice Nutrition 0.000 claims abstract description 9
- 239000003112 inhibitor Substances 0.000 claims abstract description 6
- 240000001462 Pleurotus ostreatus Species 0.000 claims abstract description 5
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims abstract description 5
- 241000609240 Ambelania acida Species 0.000 claims abstract description 4
- 241000209140 Triticum Species 0.000 claims abstract description 4
- 235000021307 Triticum Nutrition 0.000 claims abstract description 4
- 239000010905 bagasse Substances 0.000 claims abstract description 4
- 239000010902 straw Substances 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 244000082204 Phyllostachys viridis Species 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 20
- 239000002609 medium Substances 0.000 abstract description 10
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 235000001715 Lentinula edodes Nutrition 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 235000007685 Pleurotus columbinus Nutrition 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 4
- 239000000920 calcium hydroxide Substances 0.000 description 4
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000011109 contamination Methods 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- -1 KJPOa 1 g Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000024001 sorocarp development Effects 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Mushroom Cultivation (AREA)
- Fertilizers (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、椎茸のオガクズ等の培養基による人工栽培方
法に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for artificially cultivating shiitake mushrooms using a culture medium such as sawdust.
従来、椎茸類を人工培地で栽培する場合は培地を高圧滅
菌後、椎茸の種菌を植付ける方法が主な方法であった。Conventionally, when cultivating shiitake mushrooms in an artificial medium, the main method has been to sterilize the medium under high pressure and then inoculate it with shiitake mushroom seeds.
しかし、この方法では大規模な設備が必要なうえ、椎茸
菌の培養中にカビ等の雑菌による汚染を受ける恐れが大
であった。これら欠点を改善する方法として従来オガク
ズ培地に高温醗酵菌を加え、５５〜７０゛Ｃで１〜５週
間醗酵させることにより培地の滅菌を行うことなく椎茸
を人工栽培する方法が提案されているが（特開昭５７−
７４０２２号）、シかし、この方法は醗酵期間が１〜５
遇間と長く、しかも、高温醗酵菌の醗酵のみでは十分な
効果が得られないためカビ抑制剤の併用が必須の条件と
なっている。However, this method requires large-scale equipment, and there is a high risk of contamination by mold and other bacteria during culturing of the shiitake fungus. As a method to improve these drawbacks, it has been proposed to artificially cultivate shiitake mushrooms without sterilizing the medium by adding high-temperature fermentation bacteria to a sawdust medium and fermenting it at 55 to 70°C for 1 to 5 weeks. (Unexamined Japanese Patent Publication No. 57-
74022), this method requires a fermentation period of 1 to 5
Since the fermentation time is long, and fermentation using high-temperature fermentation bacteria alone does not produce sufficient effects, the combined use of mold inhibitors is an essential condition.
そこで、本発明者らは、椎茸のオガクズによる人工栽培
において、オガクズ培地の滅菌を必要としないより効果
的な方法を開発すべく研究を行なった。その結果、土壌
中から分離したバチルス属に属する高温性細菌ドア０を
使用することによりその目的を達成することができ本発
明を完成するに至った。Therefore, the present inventors conducted research to develop a more effective method for artificially cultivating shiitake mushrooms using sawdust, which does not require sterilization of the sawdust medium. As a result, by using the thermophilic bacterium Door 0 belonging to the genus Bacillus isolated from soil, the objective was achieved and the present invention was completed.
本発明は、バチルス属に属する微生物で醗酵させた培養
基にカビ抑制剤を添加することなくきのこの種苗を接種
して培養し、子実体を形成せしめるきのこの人工栽培方
法である。The present invention is a method for artificially cultivating mushrooms, in which mushroom seeds and seedlings are inoculated into a culture medium fermented with a microorganism belonging to the genus Bacillus without adding a mold inhibitor, and cultured to form fruiting bodies.
本発明の培養基としてはオガクズ、ムギワラ、バガス、
竹の粉、モミガラ等の木質、繊維質のものが用いられ、
醗酵に４５〜７５“Ｃ１１〜７日間行われる。本発明の
人工栽培方法に適用されるきのことしては食用きのこで
あればいずれの種類でもよいが、特にしいたけ、ひらた
けに適している。The culture medium of the present invention includes sawdust, wheat straw, bagasse,
Woody and fibrous materials such as bamboo powder and rice hulls are used.
Fermentation is carried out for 45-75"C11-7 days. Any type of edible mushroom can be used in the artificial cultivation method of the present invention, but Shiitake and Oyster mushrooms are particularly suitable.
本発明のオガクズ等の培養基の高温醗酵に適した微生物
は次の方法により土壌から分離した。Microorganisms suitable for high-temperature fermentation of culture media such as sawdust of the present invention were isolated from soil by the following method.
（１） 分離源：土壌
（２）培 地：Ｙｓ−寒天培地
Ｙｓ−寒天培地は酵母エキス４ｇ、でんぷん１５ｇ 、
ＫＪＰＯａ １ ｇ 、 ＭｇＳＯ４・７ＨｚＯＯ
，５ｇ １寒天２０ｇ及び水１０１００Ｏから成るもの
であって、ｐＨ７，５である。(1) Separation source: soil (2) Medium: Ys-agar medium Ys-agar medium contains 4g of yeast extract, 15g of starch,
KJPOa 1 g, MgSO4・7HzOO
, 5g 1 consists of 20g of agar and 10,100O of water, and has a pH of 7.5.
（３）培養方法：固形平面培養基に塗抹法により分離後
スラントで培養する。(3) Cultivation method: Separate by smearing on a solid flat culture medium and then culture on a slant.
この分離培地及び培養方法により、土壌から培養温度７
０°ＣでＨ−７０菌、４５゛ＣでＨ−４５菌を分離した
。With this separation medium and culture method, it is possible to grow from soil to culture temperature 7.
H-70 bacteria were isolated at 0°C, and H-45 bacteria were isolated at 45°C.
この分離菌はいずれも好気性菌であって、■−７０菌は
４５°Ｃで僅かに生育するがＨ−４５菌は７０°Ｃで生
育しない。上記Ｈ−７０菌の菌学的性質は次の通りであ
る。All of these isolated bacteria are aerobic bacteria, and the -70 bacterium grows slightly at 45°C, but the H-45 bacterium does not grow at 70°C. The mycological properties of the above H-70 bacteria are as follows.
（本頁以下余白）
形態的所見
生育の状態
■肉汁寒天平板培養（５５℃、培養３日）■肉汁寒天斜
面培養（５５’Ｃ１培養３８）■肉汁液体培養（５５℃
、培養３日）
■肉汁ゼラチン穿刺培養（５５℃、培養３日）■肉汁寒
天穿刺培養（５５℃、培養３日）■リドマスミルク （
５５℃、培養３日）生理学的性質
炭素源からの酸及びガスの生成
実験により得られた菌学的性質を、Ｂｅｒｇｅｙ’ ｓ
Ｍａｎｕａｌ ｏｆ Ｄｅｔｅｒｍｉｎａｔｉｖｅ Ｂ
ａｃｔｅｒｉｏｌｏｇｙ ８ｔｈ ｅｄ。(Margins below this page) Morphological findings Growth status ■ Meat juice agar plate culture (55℃, 3 days of culture) ■ Meat juice agar slant culture (55'C1 culture 38) ■ Meat juice liquid culture (55℃)
, culture for 3 days) ■ Meat juice gelatin puncture culture (55℃, culture for 3 days) ■ Meat juice agar puncture culture (55℃, culture for 3 days) ■ Lidomus milk (
55°C, 3 days of culture) Physiological properties The mycological properties obtained from the acid and gas production experiments from carbon sources were
Manual of Determinative B
acteriology 8th ed.
及びＢａｒｇｅｙ’ｓ Ｍａｎｕａｌ ｏｆ ５ｙｓｔ
ｅ＋ａａｔｉｃ Ｂａｃｔｅｒｏｌ。and Bargey's Manual of 5yst
e+aatic Bacterol.
−ｇｙの記載と照合したところ、Ｈ−７０株は、ダラム
陽性、桿菌、内生胞子形成、好気性、カタラーゼ陽性で
あることから、Ｂａｃｉｌｌｕｓ属に属するものとはみ
なされた。さらに、Ｈ−７０株は、６５°Ｃで生育し、
アジ化物感受性、酸性の培地で生育しないことなどから
、本株は、Ｂａｃｉｌｌｕｓ ５ｔｅａｒｏｔｈｅｒ−
肚■旦旦に概当した。When compared with the description of -gy, the H-70 strain was considered to belong to the genus Bacillus because it was Durham positive, bacillus, endospore forming, aerobic, and catalase positive. Furthermore, the H-70 strain grows at 65°C,
This strain is Bacillus 5tearother-
I guessed it roughly.
また、Ｂａｃｉｌｌｕｓ ｓｔｅａｒｏｔｈｅｒｍｏ
ｈｉｌｕｓのＴｙｐｅ−ｃｕｌｔｕｒｅであるＡＴＣＣ
１２９８０との比較を行なったところ、形態では、培養
を長（するとＨ−７０株では、細胞の長さが２０〜３０
μｌ程度に長くなる（分裂がおこりにくくなっていると
考えられた）などの現象や生理学的性質で異なる点も見
られたが、Ｎ、Ｒ，Ｓｍ１ｔｈ、 Ｒ，Ｅ、Ｇｏｒｄｏ
ｎ ａｎｄ Ｆ、Ｅ、ＣＩａｒｋ、 ＡｅｒｏｂｉｃＳ
ｐｏｒｅｆｏｒｍｉｎｇ Ｂａｃｔｅｒｉａ、 Ａｇｒ
、Ｍｏｎｏｇｒａｐｈ １６．Ｕ、Ｓ。Also, Bacillus stearothermo
ATCC, which is the type-culture of hilus
A comparison with 12980 revealed that in terms of morphology, the cell length was 20 to 30 minutes (with the H-70 strain, the cell length was 20 to 30 minutes).
There were some differences in phenomena and physiological properties, such as the length becoming about μl long (which was thought to make it difficult for division to occur), but N, R, Sm1th, R, E, Gordo
n and F, E, CIark, AerobicS
poreforming Bacteria, Agr.
, Monograph 16. U,S.
Ｄｅｐｔ、ｏｆ Ａｇｒｉｃｕｌｔｕｒｅ（１９５２）
の記載等から、Ｂａｃｉ−旦旦■並皿止肛鯰吐旦旦（７
） モ”：） ｃｈａｒａｃｔｅｒｓｖａｒｉａｔｉｏ
ｎの範晴にあると考えられた。以上より、Ｈ−７０株は
、Ｂａｃｉｌｌｕｓ ５ｔｅａｒｏｔｈｅｒａ＋ｏ ｈ
ｉｌｕｓと同定した。Dept. of Agriculture (1952)
From the descriptions of
) Mo”:) charactersvariatio
It was thought that it was in Noriharu of n. From the above, the H-70 strain is Bacillus 5tearothera+oh
It was identified as P. illus.
本発明の培養基はオガクズ、ムギワラ、バガス、竹の粉
、モミガラ等が主成分であるが、必要に応じ米糠、鼓、
醤油粕、ビタミン、ミネラル等の微量成分を添加するこ
とができる。The main ingredients of the culture medium of the present invention are sawdust, wheat straw, bagasse, bamboo powder, rice hulls, etc., but if necessary, rice bran, drum, etc.
Trace ingredients such as soy sauce lees, vitamins, and minerals can be added.
次に本発明の人工栽培法の概要を説明する。オガクズと
米糠の混合物に高温性細菌、バチルス・ステアロサーモ
フィラスＨ−７０菌を接種し、これに水酸化カルシウム
及び水を加える。このオガクズラフ０°ｃ１′ｉ〜５日
間醗酵させる。このオガクズに椎茸種菌を植付け２０〜
２５℃で３〜４ケ月間培養した後、約１０°Ｃに冷却し
て発茸させる。これを２０″Ｃに保持して子実体を生長
させ、子実体を生産し、収穫する。この方法により、培
養基のオガクズは滅菌の必要がなく、しかもカビ抑制剤
の添加も不必要である。そして、このオガクズに椎茸の
種菌を接種して他の雑菌によるコンタミを起こすことな
く、良質の椎茸が収穫できた。Next, an outline of the artificial cultivation method of the present invention will be explained. A mixture of sawdust and rice bran is inoculated with thermophilic bacteria, Bacillus stearothermophilus H-70, and calcium hydroxide and water are added thereto. This sawdust rough is fermented for 5 days from 0°c1'i. Plant Shiitake mushroom seed fungi on this sawdust for 20~
After culturing at 25°C for 3 to 4 months, the mushrooms are cooled to about 10°C and allowed to sprout. This is maintained at 20"C to grow the fruiting bodies, and the fruiting bodies are produced and harvested. By this method, the sawdust in the culture medium does not need to be sterilized, and furthermore, there is no need to add a mold inhibitor. By inoculating this sawdust with shiitake mushroom inoculum, high-quality shiitake mushrooms could be harvested without contamination with other bacteria.
（試験例）
オガクズ培養基を高温性細菌で高温醗酵を行うための好
ましい条件を調べた。(Test Example) Preferred conditions for high temperature fermentation of sawdust culture medium with thermophilic bacteria were investigated.
オガクズに米糠と水と水酸化カルシウムを加え、水分６
０〜６３％、ｐＨ７，５に調整してオガクズ培養基を調
製した。このオガクズ培養基を３００−容のポリビーカ
ー１１本に分けた。この各培養基を第１表の条件で醗酵
させた。この培養基にそれぞれ椎茸の種菌を接種し、菌
糸の繁殖の程度及び子実体の形成について調べた。結果
を第１表に示す。Add rice bran, water and calcium hydroxide to sawdust,
A sawdust culture medium was prepared by adjusting the content to 0 to 63% and pH 7.5. The sawdust culture medium was divided into 11 300-volume poly beakers. Each culture medium was fermented under the conditions shown in Table 1. Shiitake mushroom inoculum was inoculated into each culture medium, and the degree of mycelial propagation and fruiting body formation were examined. The results are shown in Table 1.
（本頁以下余白）
第１表
この表の結果からオガクズ培養基の醗酵に適した菌はバ
チルス・ステアロサーモフィラスＨ−７０菌であり、そ
の醗酵日数は１〜５日が適当であることが判った。(Margins below this page) Table 1 From the results of this table, the bacteria suitable for fermentation of sawdust culture medium is Bacillus stearothermophilus H-70, and the appropriate fermentation period is 1 to 5 days. It turns out.
本発明はオガクズ等の培養基をバチルス属に属する微生
物、例えばバチルス・ステアロサーモフィラスＨ−７０
菌で高温醗酵することにより培養基を従来法のように予
め滅菌する必要がなく、しかもカビ抑制剤の添加の必要
もない。そして、この培養基を使用してカビ等によるコ
ンタミを起こすことなく、良質のきのこ、例えば椎茸を
収穫できる。The present invention uses a culture medium such as sawdust for microorganisms belonging to the genus Bacillus, such as Bacillus stearothermophilus H-70.
By fermenting at high temperatures with bacteria, there is no need to previously sterilize the culture medium as in conventional methods, and there is no need to add mold inhibitors. By using this culture medium, high-quality mushrooms, such as shiitake mushrooms, can be harvested without causing contamination by mold or the like.
更にこの方法の利点は従来のオガクズ培養基の高温醗酵
の例に比して醗酵時間が１〜５日間と短期間ですむこと
である。また、この方法は上記特徴からしてきのこの栽
培操作が面単であるばかりでなく経費が安くてすむこと
から経済的方法である。A further advantage of this method is that the fermentation time is short, 1 to 5 days, compared to conventional high temperature fermentation using sawdust culture media. Moreover, this method is an economical method because the mushroom cultivation operation is not only simple but also inexpensive due to the above-mentioned characteristics.
次に実施例により本発明の詳細な説明する。但し、本発
明はこの実施例により限定されるものでない。Next, the present invention will be explained in detail with reference to Examples. However, the present invention is not limited to this example.
実施例１
オガクズ３００ｇにバチルス・ステアロサーモフィラス
Ｈ−７０（微工研菌寄第１００１０号）を加え、米糠３
０ｇ、水酸化カルシウム１８ｇ及び水２４０ｄを加え７
０°Ｃで３日間醗酵させた。この醗酵オガクズに市販の
椎茸の種菌を接種し、２０〜２５°Ｃで３〜４ヶ月保持
した。次いでこれを約１０°Ｃに１０日冷却して発茸さ
せ、２０“Ｃに７日保持して子実体を成育させて椎茸１
１０ｇを収穫した。Example 1 Bacillus stearothermophilus H-70 (Feikoken Bacteria No. 10010) was added to 300 g of sawdust, and 300 g of rice bran was added.
0g, add 18g of calcium hydroxide and 240d of water 7
Fermentation was performed at 0°C for 3 days. This fermented sawdust was inoculated with commercially available shiitake mushroom inoculum and kept at 20-25°C for 3-4 months. Next, the mushrooms were cooled to about 10°C for 10 days to germinate, and kept at 20°C for 7 days to grow fruiting bodies and produce 1 shiitake mushroom.
10g was harvested.
実施例２
オガクズ３００ｇにバチルス・ステアロサーモフィラス
Ｈ−７０（微工研菌寄第１００１０号）を加え、米Ｈ３
０ｇ、水酸化カルシウム１８ｇ及び水２４０戚を加えて
７０°Ｃで３日間醗酵させた。Example 2 Bacillus stearothermophilus H-70 (Feikoken Bacteria No. 10010) was added to 300 g of sawdust, and rice H3
0 g, 18 g of calcium hydroxide, and 240 g of water were added and fermented at 70°C for 3 days.
この醗酵オガクズに市販のひらたけの種菌を接種し、２
０〜２５゛Ｃで１ケ月保持した。This fermented sawdust was inoculated with a commercially available oyster mushroom starter, and 2
It was kept at 0-25°C for 1 month.
次いでこれを約１０°Ｃに１０日冷却して発茸させ、１
５°Ｃに８日保持して子実体を成育させて、ひらたけ１
３０ｇを収穫した。Next, the mushrooms were cooled to about 10°C for 10 days to sprout, and 1
Hold at 5°C for 8 days to grow fruiting bodies and grow Hiratake 1.
30g was harvested.
Claims (6)
カビ抑制剤を添加することなくきのこの種菌を接種して
培養し、子実体を形成せしめることを特徴とするきのこ
の人工栽培方法。(1) A method for artificially cultivating mushrooms, which comprises inoculating and culturing mushroom inoculum into a culture medium fermented with microorganisms belonging to the genus Bacillus without adding a mold inhibitor to form fruiting bodies.
モミガラであることを特徴とする請求項１記載のきのこ
の人工栽培方法。(2) The culture medium is sawdust, wheat straw, bagasse, bamboo powder,
2. The method for artificially cultivating mushrooms according to claim 1, wherein the mushrooms are rice husks.
とする請求項１記載のきのこの人工栽培方法。(3) The method for artificially cultivating mushrooms according to claim 1, wherein the fermentation is carried out at 45 to 75°C for 1 to 7 days.
サーモフィラスであることを特徴とする請求項１記載の
きのこの人工栽培方法。(4) The method for artificially cultivating mushrooms according to claim 1, wherein the microorganism belonging to the genus Bacillus is Bacillus stearothermophilus.
ステアロサーモフィラスＨ−７０であることを特徴とす
る請求項１記載のきのこの人工栽培方法。(5) Bacillus stearothermophilus is Bacillus stearothermophilus
2. The method for artificially cultivating mushrooms according to claim 1, wherein the mushroom is Stearothermophilus H-70.
する請求項１記載のきのこの人工栽培方法。(6) The method for artificially cultivating mushrooms according to claim 1, wherein the mushrooms are Shiitake mushrooms or Hiratake mushrooms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63119376A JP2637470B2 (en) | 1988-05-18 | 1988-05-18 | Mushroom artificial cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63119376A JP2637470B2 (en) | 1988-05-18 | 1988-05-18 | Mushroom artificial cultivation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01291726A true JPH01291726A (en) | 1989-11-24 |
| JP2637470B2 JP2637470B2 (en) | 1997-08-06 |
Family
ID=14759979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63119376A Expired - Lifetime JP2637470B2 (en) | 1988-05-18 | 1988-05-18 | Mushroom artificial cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2637470B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5364788A (en) * | 1991-07-01 | 1994-11-15 | Ahc Inc. | Pure culture of Bacillus subtilis FERM BP-3418 |
| JP2016041027A (en) * | 2014-08-15 | 2016-03-31 | マッシュトレーディング株式会社 | Mushroom cultivation method, fermentation mushroom bed generating mixer, and mushroom cultivation system |
| JPWO2021230248A1 (en) * | 2020-05-12 | 2021-11-18 | ||
| CN115024160A (en) * | 2022-06-24 | 2022-09-09 | 河南农业大学 | Method for reducing cadmium content of mushroom hyphae under cadmium stress |
-
1988
- 1988-05-18 JP JP63119376A patent/JP2637470B2/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5364788A (en) * | 1991-07-01 | 1994-11-15 | Ahc Inc. | Pure culture of Bacillus subtilis FERM BP-3418 |
| JP2016041027A (en) * | 2014-08-15 | 2016-03-31 | マッシュトレーディング株式会社 | Mushroom cultivation method, fermentation mushroom bed generating mixer, and mushroom cultivation system |
| JPWO2021230248A1 (en) * | 2020-05-12 | 2021-11-18 | ||
| WO2021230248A1 (en) * | 2020-05-12 | 2021-11-18 | 株式会社楽々 | Culture medium, fungus bed, bagged fungus bed, culture medium manufacturing method, fungus bed manufacturing method, bagged fungus bed manufacturing method |
| CN115426872A (en) * | 2020-05-12 | 2022-12-02 | 株式会社乐乐 | Culture medium, fungal bed, bagged fungal bed, culture medium production method, fungal bed production method, and bagged fungal bed production method |
| CN115024160A (en) * | 2022-06-24 | 2022-09-09 | 河南农业大学 | Method for reducing cadmium content of mushroom hyphae under cadmium stress |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2637470B2 (en) | 1997-08-06 |
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