CN109729929A - A method of bacterium material production Armillaria mellea is discarded using Rhizoma Gastrodiae - Google Patents
A method of bacterium material production Armillaria mellea is discarded using Rhizoma Gastrodiae Download PDFInfo
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- CN109729929A CN109729929A CN201910225808.2A CN201910225808A CN109729929A CN 109729929 A CN109729929 A CN 109729929A CN 201910225808 A CN201910225808 A CN 201910225808A CN 109729929 A CN109729929 A CN 109729929A
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- bacterium material
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Abstract
The invention belongs to Rhizoma Gastrodiaes to discard bacterium material production reutilization technology field, in particular a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae, include the following steps: that S1, Rhizoma Gastrodiae discard the processing of bacterium material fritter, the processing of S2, nutrient solution, the preparation of S3, composite nutrient-fluid, the processing of S4, bacterium material fritter matrix, S5, halimasch inoculated and cultured, S6, the observation of halimasch cultural colony and evaluation index determination, the screening and determination of S7, preferably nutrient solution prescription；The production of Armillaria mellea is carried out with the processing method, tradition is changed using the method for fresh sawdust and branch production halimasch inoculation, pass through the supplement of nutrient solution, it can be realized Rhizoma Gastrodiae and discard again utilization of the bacterium material in Armillaria mellea production, the utilization rate of timber resources is improved, the cost of traditional Armillaria mellea production is reduced.
Description
Technical field
The invention belongs to Rhizoma Gastrodiaes to discard bacterium material production reutilization technology field, and in particular to a kind of to discard bacterium material using Rhizoma Gastrodiae
The method for producing Armillaria mellea.
Background technique
Halimasch is symbiotic effects important in Rhizoma Gastrodiae growth course, which decomposes the cellulose and lignin in timber
Make to form certain symbiosis as nutrition needed for own growth and development, while with Rhizoma Gastrodiae, is Rhizoma Gastrodiae in different growths
Stage provides nutrition, guarantees normal growth and the development of Rhizoma Gastrodiae.Therefore, the cultivation of Rhizoma Gastrodiae be unable to do without excellent Armillaria mellea,
In Yunnan tall gastrodia tuber main producing region, the production of Armillaria mellea has become a kind of industry, and the investment of strain is gastrodin cultivation process
In one of prime cost.The production of Armillaria mellea at present is mainly with cotton seed hulls, weed tree sawdust, fresh timber fritter for main material
Material, Yunnan Province cotton seed hulls transportation cost is higher, increases for a long time dependent on fresh timber and weed tree sawdust as main matrix gloomy
The protection pressure of woods ecological resources, traditional halimasch production method are unfavorable for reducing the cultivation cost of Rhizoma Gastrodiae, improve Rhizoma Gastrodiae
Plant profit.So finding new material and method substitutes traditional halimasch production, it is the needs of Modern Green agricultural development.
Summary of the invention
To solve the problems mentioned above in the background art.Bacterium material production honey is discarded using Rhizoma Gastrodiae the present invention provides a kind of
The characteristics of method of ring bacterium strain has and recycles waste wood, reduces cultivation cost.
To achieve the above object, the invention provides the following technical scheme: a kind of discard bacterium material production halimasch using Rhizoma Gastrodiae
The method of strain, includes the following steps:
S1, Rhizoma Gastrodiae discard the processing of bacterium material fritter；
The processing of S2, nutrient solution；
The preparation of S3, composite nutrient-fluid；
The processing of S4, bacterium material fritter matrix；
S5, halimasch inoculated and cultured；
S6, the observation of halimasch cultural colony and evaluation index determination；
The screening and determination of S7, preferably nutrient solution prescription.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S1: it is mainly Qinggang wood or cork oak that Rhizoma Gastrodiae, which discards bacterium material, after removing discarded bacterium material surface soil, is handled as long 1-2cm,
The fritter of wide 1cm or so is spare.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S2: according to potato 250g/L, corn 80g/L, soybean 80g/L amount carry out nutrient solution preparation, by corn flour, soy meal,
1L tap water is added according to formula in three kinds of materials of potato fritter, boils 20 minutes and filters, filtrate is standby as nutrient solution to be added
With PH is natural.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S3: three kinds of corn and soybean, potato nutrient solutions prepared being carried out mixture according to the ratio column of 1:1 (volume) respectively, are finally made
It is spare at three kinds of corn+soybean, corn+potato, potato+soybean mixed nutrient solutions, with fresh bacterium material+tap water, discarded bacterium material+
Tap water is compareed as method.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S4: bacterium material fritter is packed into high 9cm by the amount of every bottle of 100g, and the tissue culture bottle of internal diameter 5cm is separately added into the different mixing of 150ml
Nutrient solution, impregnate 48h after sterilize it is spare.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S5: the bacterium material fritter after sterilizing being put into super bacterium platform, under aseptic condition, the halimasch of 0.5x0.5cm is accessed in every bottle of bacterium material
It 5 pieces of strain fritter, is put into 25 DEG C of incubators after tissue culture bottle closure and cultivates, while observing culture situation.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S6: in Armillaria mellea incubation, with mycelium germination time, Mycelium growth rate, mycelium morphology etc. for bacterium material base
The primary evaluation index of matter, culture observation 30 days, observation and record index are foundation, and it is raw to filter out most suitable armillaria mycelium
Long addition formula determines and discards bacterium material as main material using Rhizoma Gastrodiae, and after addition nutrient solution processing, Armillaria mellea growth is best
Formula and method.
It is described as a kind of method optimal technical scheme for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae of the invention
In S7: being cultivated 30 days under the conditions of 20 DEG C, the influence of speed and form that different substrates processing method grows armillaria mycelium.
Compared with prior art, the beneficial effects of the present invention are:
Carry out Armillaria mellea production 1. discarding bacterium material with Rhizoma Gastrodiae and substituting traditional cotton seed hulls, weed tree sawdust, segment wood, reduces
Dependence of traditional Armillaria mellea production technology to cotton seed hulls and fresh timber reduces Yunnan tall gastrodia tuber cultivation to a certain extent
The production cost in area, improves the utilization rate of forest tree resource.
2. preparing material using potato, soybean, corn as nutrient solution, the battalion that Rhizoma Gastrodiae is discarded in bacterium material is supplemented and enriched
It forms point, promotes strain and sprout, enhance growth tendency of the mycelium in matrix, ensure that the normal growth of halimasch.
3. the discarded bacterium material in Rhizoma Gastrodiae producing region is rationally utilized, circulation of the discarded bacterium material in Armillaria mellea production is realized
It utilizes, has pushed the development of green agriculture.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is step schematic diagram of the invention.
Fig. 2 is that the discarded bacterium material comparison culture of Rhizoma Gastrodiae is shone after Different Nutrition liquid of the invention is handled.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of to discard bacterium material production Armillaria mellea using Rhizoma Gastrodiae
Method includes the following steps:
S1, Rhizoma Gastrodiae discard the processing of bacterium material fritter；
The processing of S2, nutrient solution；
The preparation of S3, composite nutrient-fluid；
The processing of S4, bacterium material fritter matrix；
S5, halimasch inoculated and cultured；
S6, the observation of halimasch cultural colony and evaluation index determination；
The screening and determination of S7, preferably nutrient solution prescription are specific.
Specifically, in the S1: it is mainly Qinggang wood or cork oak that Rhizoma Gastrodiae, which discards bacterium material, removes discarded bacterium material surface mud
It after soil, handles as long 1-2cm, the fritter of wide 1cm or so is spare.
Specifically, in the S2: carrying out matching for nutrient solution according to the amount of potato 250g/L, corn 80g/L, soybean 80g/L
Three kinds of corn flour, soy meal, potato fritter materials are added 1L tap water according to formula, boil 20 minutes and filter, filtrate is made by system
Spare for nutrient solution to be added, PH is natural.
Specifically, in the S3: by three kinds of corn and soybean, potato nutrient solutions prepared respectively according to 1:1's (volume)
Mixture is carried out than column, it is spare to be eventually fabricated three kinds of corn+soybean, corn+potato, potato+soybean mixed nutrient solutions, with fresh bacterium
Material+tap water, discarded bacterium material+tap water are compareed as method.
Specifically, in the S4: bacterium material fritter is packed into high 9cm, the tissue culture bottle of internal diameter 5cm, difference by the amount of every bottle of 100g
The different nutrient solution of 150ml is added, sterilizes after immersion 48h spare.
Specifically, in the S5: the bacterium material fritter after sterilizing being put into super bacterium platform, under aseptic condition, is connect in every bottle of bacterium material
Enter 5 pieces of Armillaria mellea fritter of 0.5x0.5cm, is put into 25 DEG C of incubators and cultivates after tissue culture bottle closure, while observing culture
Situation.
Specifically, in the S6: in Armillaria mellea incubation, with mycelium germination time, Mycelium growth rate, bacterium
Filament form etc. is the primary evaluation index of bacterium material matrix, and culture observation 30 days, observation and record index are foundation, is filtered out most
It is suitble to the addition formula of armillaria mycelium growth, determines and bacterium material is discarded as main material using Rhizoma Gastrodiae, after adding nutrient solution processing,
Armillaria mellea grows best formula and method.
Specifically, in the S7: being cultivated 30 days under the conditions of 20 DEG C, different substrates processing method grows armillaria mycelium
Speed and form influence, please refer to the influence that 1 different disposal method of table grows halimasch.
The influence table that the different bacterium material processing methods of table 1 grow armillaria mycelium
Referring to Fig. 2, being cultivated 30 days under the conditions of 20 DEG C, the speed that different substrates processing method grows armillaria mycelium
With the influence of form, it is respectively as follows: potato+soybean, soybean+corn, potato+corn, fresh bacterium material+originally in figure from left to right
Water, discarded bacterium material+tap water.
The beneficial effects of the present invention are:
Carry out Armillaria mellea production 1. discarding bacterium material with Rhizoma Gastrodiae and substituting traditional cotton seed hulls, weed tree sawdust, segment wood, reduces
Dependence of traditional Armillaria mellea production technology to cotton seed hulls and fresh timber reduces Yunnan tall gastrodia tuber cultivation to a certain extent
The production cost in area, improves the utilization rate of forest tree resource.
2. preparing material using potato, soybean, corn as nutrient solution, the battalion that Rhizoma Gastrodiae is discarded in bacterium material is supplemented and enriched
It forms point, promotes strain and sprout, enhance growth tendency of the mycelium in matrix, ensure that the normal growth of halimasch.
3. the discarded bacterium material in Rhizoma Gastrodiae producing region is rationally utilized, circulation of the discarded bacterium material in Armillaria mellea production is realized
It utilizes, has pushed the development of green agriculture.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (8)
1. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae, which comprises the steps of:
S1, Rhizoma Gastrodiae discard the processing of bacterium material fritter；
The processing of S2, nutrient solution；
The preparation of S3, composite nutrient-fluid；
The processing of S4, bacterium material fritter matrix；
S5, halimasch inoculated and cultured；
S6, the observation of halimasch cultural colony and evaluation index determination；
The screening and determination of S7, preferably nutrient solution prescription.
2. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S1: it is mainly that Qinggang wood or cork oak are handled after removing discarded bacterium material surface soil as long 1- that Rhizoma Gastrodiae, which discards bacterium material,
The fritter of 2cm, wide 1cm or so are spare.
3. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S2: the preparation of nutrient solution is carried out according to the amount of potato 250g/L, corn 80g/L, soybean 80g/L, by corn flour, soybean
1L tap water is added according to formula in three kinds of powder, potato fritter materials, boils 20 minutes and filters, filtrate is as nutrient solution to be added
Spare, PH is natural.
4. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S3: three kinds of corn and soybean, potato nutrient solutions prepared being carried out mixture according to the ratio column of 1:1 (volume) respectively, most
After to be made three kinds of corn+soybean, corn+potato, potato+soybean mixed nutrient solutions spare, with fresh bacterium material+tap water, discarded
Bacterium material+tap water is compareed as method.
5. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S4: bacterium material fritter is packed into high 9cm by the amount of every bottle of 100g, and it is different to be separately added into 150ml for the tissue culture bottle of internal diameter 5cm
Mixed nutrient solution, impregnate 48h after sterilize it is spare.
6. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S5: the bacterium material fritter after sterilizing being put into super bacterium platform, under aseptic condition, the honey of 0.5x0.5cm is accessed in every bottle of bacterium material
It 5 pieces of fritter of ring bacterium strain, is put into 25 DEG C of incubators after tissue culture bottle closure and cultivates, while observing mycelium germination, growth, form etc.
Cultivate situation.
7. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S6: in Armillaria mellea incubation, with mycelium germination time, Mycelium growth rate, mycelium morphology etc. for bacterium
The primary evaluation index of material matrix, culture observation 30 days, using observation and record index as foundation, filters out most suitable halimasch bacterium
The addition formula of mycelial growth determines and discards bacterium material as main material using Rhizoma Gastrodiae, and after addition nutrient solution processing, Armillaria mellea is raw
Long best formula and method.
8. a kind of method for discarding bacterium material production Armillaria mellea using Rhizoma Gastrodiae according to claim 1, which is characterized in that
In the S7: it is cultivated 30 days under the conditions of 20 DEG C, speed that different substrates processing method grows armillaria mycelium and form
It influences.
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Cited By (3)
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| CN111019835A (en) * | 2019-11-01 | 2020-04-17 | 汉中市汉台区汉麓生物科技有限公司 | Method for separating Armillaria mellea from ambary endophytic fungi |
| CN111109009A (en) * | 2019-12-11 | 2020-05-08 | 西南林业大学 | Armillaria mellea SWFU-09 and application thereof |
| CN115336503A (en) * | 2022-08-30 | 2022-11-15 | 西南林业大学 | Preparation method of substitute bacterial material for gastrodia elata cultivation |
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