CN103931566B - A kind of method inducing oriental tobacco budworm diapause - Google Patents
A kind of method inducing oriental tobacco budworm diapause Download PDFInfo
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- CN103931566B CN103931566B CN201410170218.1A CN201410170218A CN103931566B CN 103931566 B CN103931566 B CN 103931566B CN 201410170218 A CN201410170218 A CN 201410170218A CN 103931566 B CN103931566 B CN 103931566B
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Abstract
A kind of method inducing oriental tobacco budworm diapause, belongs to pest control technical field.Comprising the following steps: 1) oriental tobacco budworm newly hatched larvae supports to 3 ages at a diameter of 10 15cm culture dish man-made feeds, then raise in 1.2cm × 10cm teat glass, temperature pre-control be made as 20 25 DEG C, Light To Dark Ratio be 8 11:13 16, light intensity is 25 35%, standby；2) then insect in step 1) being moved to 45 DEG C, relative humidity is the interior raising of growth cabinet 10 12 days of 78 86%, is placed in the refrigerator of 0 DEG C raising 15 18 days again, repeats to process 23 times, standby；3) finally by step 2) in process after insect move to 3 4d raised by 25 32 DEG C of casees, the most normally cultivate, oriental tobacco budworm diapause can be made.A kind of above-mentioned method inducing oriental tobacco budworm diapause, easy to operate, low cost, effective, any adverse effect will not be produced.
Description
Technical field
The invention belongs to pest control technical field, be specifically related to a kind of method inducing oriental tobacco budworm diapause.
Background technology
Oriental tobacco budworm has another name called Heliothis assulta or tobacco budworm, and classification belongs to Lepidoptera, Noctuidae.Oriental tobacco budworm distribution is wide, feeding habits are miscellaneous, reproductive capacity is strong, strong stress resistance, also have the characteristic of diapause, the most serious to endanger Nicotiana tabacum L..Many places Nicotiana tabacum L. producing region occurrence injury is serious in recent years, such as Guizhou, Sichuan, Central China and the Yellow River and Huai He River cigarette district.Being endangered by oriental tobacco budworm, general time Nicotiana tabacum L. lost units 5%-10%, outbreak year part can be more than 15%.The harm of oriental tobacco budworm is that the blade of its larval feeding host, flower, flower bud, fruit etc. cause, and therefore instar larvae and generation directly influence the extent of injury to crop.Because each department host is different, and different regions different geographic populations such as there are differences at the many factors, causes different regions the most different to the report of oriental tobacco budworm instar larvae.When ambient environmental conditions is suitable, insecticide can continuously breed by growth promoter, and when condition changes and survives unfavorable to it, insecticide can grow by growth arrest, and this state is exactly diapause.Insecticide enters diapause needs to accept specific signal, and this signal is typically derived from environment.Temperature is the key factor that induction insecticide enters diapause, and mostly laboratory is to utilize temperature to induce insecticide.
In diapause at the beginning, insecticide in order to avoid the low temperature unfavorable damage brought to body during this, internal can improve cold resistant metabolism adjust.Studies have found that, polyhydric alcohol (glycerol, mannitol, Sorbitol, threitol etc.) and saccharide (trehalose, sucrose, fructose, glucose etc.) accumulation are substantially, these changes can help insecticide to improve cold tolerance by reducing freezing point and supercooling point, when polyhydric alcohol concentration culminates, insecticide is just provided with cold tolerance.Recently some researchs find, the drought resisting factor of insecticide also includes some albumen and aminoacid, and many diapause insecticides can produce antifreeze protein (AFP), antifreeze peptide can reduce the supercooling point of body fluid.But through finding deeper into concrete research, these antifreeze proteins can not make insecticide improve frost resistance, but by being adsorbed onto newly formed ice crystal surface, stops the growth of ice-nucleus, to reduce and to stablize supercooling state.Though arrest of development during Insect Diapause, diapause and metabolism and breeding are connected, and after diapause, the biological characteristics such as the life-span of insecticide, reproductivity and Offspring's deyelopment and diapause generation and diapause duration have relation.The generation of diapause is influential on the time-to-live after Insect Diapause, and typically living through the life-span after the individual diapause of diapause can be shorter than the individuality not having diapause, and diapause duration also influences whether the life after Insect Diapause.Although some insecticide experience termination of diapause, but if subjected to the impact again of envirment factor, diapause can be again introduced into.Diapause not only produces impact to the mother of diapause in generation, and also can affect its filial generation, such as on the developmental rate reducing filial generation, the reproductivity that affects filial generation.The present invention carries out Diapause inducement by certain inductive technology to oriental tobacco budworm, destroys its physiological function, reduces the developmental rate of its filial generation, affects the reproductivity of filial generation, and the prevention and control to oriental tobacco budworm have great importance.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design and the technical scheme of a kind of method inducing oriental tobacco budworm diapause is provided, i.e. can be reached prevention and control insect purpose by simple process, and the method is easy to operate, low cost, effective, any adverse effect will not be produced.
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that comprise the following steps:
1) oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 10-15cm culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 20-25 DEG C, Light To Dark Ratio be 8-11:13-16, light intensity is 25-35%, standby；
2) then insect in step 1) is moved to 4-5 DEG C, relative humidity be 78-86% growth cabinet in raise 10-12 days, again be placed in the refrigerator of 0 DEG C raising 15-18
My god, repeat to process 2-3 time, standby；
3) finally by step 2) in process after insect move in 25-32 DEG C of case raise 3-4d, the most normally cultivate, oriental tobacco budworm diapause can be made.
A kind of described method inducing oriental tobacco budworm diapause, its feature is in step 1): in raising temperature pre-control at 21-24 DEG C, preferably 22-23
℃。
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that in step 1): Light To Dark Ratio is 9-10:12-15.
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that in step 1): light intensity is 28-30%.
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that step 2) in: growth cabinet temperature is 4.5 DEG C, and relative humidity is 80-85%, and feeding time is 11 days.
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that step 2) in: it is placed in the refrigerator of 0 DEG C raising 16-17 days again.
A kind of described method inducing oriental tobacco budworm diapause, it is characterised in that in step 3): raising temperature is 28-30 DEG C.
A kind of above-mentioned method inducing oriental tobacco budworm diapause, different variations in temperature is utilized to stimulate the physiological function destroying insect, induction oriental tobacco budworm diapause, shorten insect bulk life time and destroy its reproduction ability, to reduce the developmental rate of its filial generation, to affect the reproductivity of its filial generation, i.e. can be reached prevention and control insect purpose by simple process, and the method is easy to operate, low cost, effective, any adverse effect will not be produced.
Detailed description of the invention
In conjunction with specific embodiment, the present invention is further illustrated below.
Embodiment 1
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 10cm culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 20 DEG C, Light To Dark Ratio be 10:14, light intensity is 33%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 78%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 2 times, and the insect after finally processing moves to process at 25 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Embodiment 2
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 15m culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 25 DEG C, Light To Dark Ratio be 10:14, light intensity is 31%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 86%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 3 times, and the insect after finally processing moves to process at 32 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Embodiment 3
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 13m culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 21 DEG C, Light To Dark Ratio be 10:14, light intensity is 29%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 80%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 3 times, and the insect after finally processing moves to process at 27 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Embodiment 4
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 12m culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 22 DEG C, Light To Dark Ratio be 10:14, light intensity is 27%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 82%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 2 times, and the insect after finally processing moves to process at 29 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Embodiment 5
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 11m culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 23 DEG C, Light To Dark Ratio be 10:14, light intensity is 35%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 84%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 3 times, and the insect after finally processing moves to process at 30 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Embodiment 6
Oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 10cm culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 24 DEG C, Light To Dark Ratio be 10:14, light intensity is 25%.Then insect moving to 4 DEG C, relative humidity is the interior process of growth cabinet 10 days of 85%, is placed in the refrigerator of 0 DEG C process 15 days again, repeats to process 2 times, and the insect after finally processing moves to process at 31 DEG C 3d.The most normally cultivate, oriental tobacco budworm diapause can be made.
Beneficial effects of the present invention is further illustrated below by way of test
Table 1
Embodiment 1-6 processes lower oriental tobacco budworm diapause result
| Process | Worm pupa number | Non-diapause pupae original body mass (mg) | Diapause pupae original body mass (mg) |
| Embodiment 1 | 50 | 273.22±5.95 | 238.38±6.27 |
| Embodiment 2 | 50 | 276.04±4.38 | 231.41±4.88 |
| Embodiment 3 | 50 | 283.30±3.83 | 234.47±2.5 |
| Embodiment 4 | 50 | 284.45±2.16 | 239.28±4.13 |
| Embodiment 5 | 50 | 282.16±3.27 | 230.26±5.57 |
| Embodiment 6 | 50 | 279.42±4.10 | 235.35±3.15 |
Note: in table, data are meansigma methods ± standard error (Mean ± SE)
Result of the test shows: the oriental tobacco budworm growth promoter after embodiment 1-6 processes the most substantially is suppressed, and it can be produced diapause effect, and wherein embodiment 2 is better than embodiment 1, and embodiment 3 is better than embodiment 4, and embodiment 5 is better than embodiment 6.
Claims (5)
1. the method inducing oriental tobacco budworm diapause, it is characterised in that comprise the following steps:
1) oriental tobacco budworm newly hatched larvae is supported to 3 ages at a diameter of 10-15cm culture dish man-made feeds, then in 1.2cm × 10cm teat glass raise, temperature pre-control be made as 20-21 DEG C, Light To Dark Ratio be 8-11:13-16, light intensity is 25-35%, standby；
2) then insect in step 1) being moved to 4-5 DEG C, relative humidity is growth cabinet interior raising 10-12 days of 82-86%, is placed in the refrigerator of 0 DEG C raising 15-18 days again, repeats to process 2-3 time, standby；
3) finally by step 2) in process after insect move in 25-32 DEG C of case raise 3-4d, the most normally cultivate, oriental tobacco budworm diapause can be made.
A kind of method inducing oriental tobacco budworm diapause the most as claimed in claim 1, it is characterised in that in step 1): light intensity is 28-30%.
A kind of method inducing oriental tobacco budworm diapause the most as claimed in claim 1, it is characterised in that step 2) in: growth cabinet temperature is 4.5 DEG C, and relative humidity is 84-85%, and feeding time is 11 days.
A kind of method inducing oriental tobacco budworm diapause the most as claimed in claim 1, it is characterised in that step 2) in: it is placed in the refrigerator of 0 DEG C raising 16-17 days again.
A kind of method inducing oriental tobacco budworm diapause the most as claimed in claim 1, it is characterised in that in step 3): raising temperature is 28-30 DEG C.
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| CN104255669B (en) * | 2014-09-24 | 2017-01-18 | 中国农业科学院植物保护研究所 | Diapause induction method of harmonia dimidiata |
| CN104351140B (en) * | 2014-09-24 | 2017-10-17 | 中国农业科学院植物保护研究所 | Red shoulder ladybug Diapause termination method |
| CN108157399A (en) * | 2017-12-11 | 2018-06-15 | 常州市协旺纺织品有限公司 | It is a kind of to be used to prevent preparation of oriental tobacco budworm and preparation method thereof |
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